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Articles 61 - 64 of 64
Full-Text Articles in Chemicals and Drugs
Fatty Liver And Metabolism Of Xenobiotics In The Rat, Joyce D. Stone
Fatty Liver And Metabolism Of Xenobiotics In The Rat, Joyce D. Stone
Theses and Dissertations (ETD)
No abstract provided.
Characteristics Of The Recovery Of The Coenzyme A-Synthesizing Protein Complex From Saccharomyces Cerevisiae, Stanley Joseph Tarnowski Jr.
Characteristics Of The Recovery Of The Coenzyme A-Synthesizing Protein Complex From Saccharomyces Cerevisiae, Stanley Joseph Tarnowski Jr.
Theses and Dissertations (ETD)
A multienzyme complex contained in Bakers' yeast (Saccharomyces cerevisiae) which synthesizes CoA has been named the coenzyme A-synthesizing protein complex (CoA-SPC). The CoA-SPC has been shown to be insoluble in the crude Bakers' yeast cell lysate formed by exposing the yeast cell to ether and dry ice. Only after solubilization has this multienzyme complex been shown to catalyze the formation of bound dephospho-CoA utilizing the substrates adenosine triphosphate, D-pantothenic acid and L-cysteine. A low molecular weight component or components of the soluble fraction of the yeast cell and chloride ion appears to be responsible for the solubilization of CoA-SPC. This …
The Synthesis Of Active Site Directed Inhibitors For The Lac Permease Of Escherichia Coli, Mahmoud Ahmed
The Synthesis Of Active Site Directed Inhibitors For The Lac Permease Of Escherichia Coli, Mahmoud Ahmed
Theses and Dissertations (ETD)
No abstract provided.
The Purification, Properties And Subunit Structure Of Glycerol Dehydrogenase, Michael James Barrett
The Purification, Properties And Subunit Structure Of Glycerol Dehydrogenase, Michael James Barrett
Theses and Dissertations (ETD)
The inducible, NAD-linked glycerol dehydrogenase (E.C. 1.1.1.6) of a guanine requiring mutant of A. aerogenes has been purified to homogeneity. The molecular weight of the pure enzyme was found to be 3.4 x 105 daltons. The sedimentation coefficient of the enzyme was 10.7 x 10-13 sec.-1. The diffusion constant was 3.07 x 10-7 cm2/sec.
The partial specific volume calculated from the amino acid composition was 0.72 ml/g. The following kinetic parameters were determined; Km glycerol, 2.4 x 10-3 m, Km NAD, 2.8 x 10-4 M, Km dihydroxyacetone, 5.1 …