Chemicals and Drugs Commons^™

Effects Of Sodium Intake, Furosemide, And Infusion Of Atrial Natriuretic Peptide On The Urinary And Metabolic Clearances Of Arginine Vasopressin In Normal Subjects, Arnold M. Moses, Carol Jones, Carolyn B. Yucha

Nursing Faculty Research

Arginine vasopressin (AVP) and atrial natriuretic peptide (ANP) have important influences on water and electrolyte metabolism, and studies on the interactions between these hormones may have important implications. We have investigated the effects of sodium intake, furosemide, and infusion of ANP on the urinary and metabolic (nonurinary) clearances of AVP in hydrated normal subjects. On a high sodium diet there was an increase in urine volume, sodium excretion, osmolal clearance, plasma ANP concentration, and urinary clearance and fractional excretion of AVP, with a decrease in PRA. The infusion of furosemide increased urine volume, sodium excretion, osmolal clearance, and PRA, but …

Microwell Based Competitive Enzyme Immunoassay For Quantifying Serum Triiodothyronine And Thyroxine Levels, Yiwen Yang

Chemistry & Biochemistry Theses & Dissertations

A microwell based competitive enzyme immunoassay for determination of total thyroxine and triiodothyronine in human serum was developed with use of horseradish peroxidase conjugate. The assay, which can be performed in only 1.5 hours at room temperature, involves simultaneous incubation of T₃- or T₄- HRPO conjugate and serum sample in anti-T₃ or anti-T₄ gamma globulin fraction coated polystyrene microwells.

Isolation of anti-T₃ and anti-T₄ gamma globulin fractions were accomplished from whole antiserum by using DEAE-cellulose chromatograph. A carbodiimide coupling method was described to prepare the T₄- and T₃-HRPO …

Autonomic Nervous System And Cholesterol Transport, Yuan-Line Hung

Loma Linda University Electronic Theses, Dissertations & Projects

The effect of autonomic nervous system on cholesterol transport is of clinical interest because the relationship between lipoprotein metabolism and atherogenesis is expressed in coronary artery disease.

Generally speaking, beta blockers increase serum VLDL level but decrease both HDL and HDL₂ cholesterol levels without affecting the LDL in humans. We are unaware of reports concerning the effects of metoprolol on lipoprotein metabolism nor comparison of the effects of metoprolol and propranolol in the unhandled rat model. In part I we employed the unhandled rats to compared the effects of these 2 drugs administered with the drinking water in order …

Hypoxanthine-Induced Differentiation Of Cultured Human Leukemia Cells, Gayle Jennette Singleton

Chemistry & Biochemistry Theses & Dissertations

Human cultured leukemia cells appear to have a decreased amount of inosine in their tRNA. When cells with inosine deficient tRNA are placed in a hypoxanthine fortified media, they incorporate hypoxanthine into their tRNA by the action of the enzyme tRNA-hypoxanthine ribosyl transferase. This generates the nucleoside inosine in the tRNA. The cultured human leukemia cell lines, CCRF-CEM, HL-60, and HGPRT(-) HL- 60, incorporate hypoxanthine into their tRNA, as determined by tRNA isolation, hydrolysis, and HPLC analysis. Hypoxanthine treatment dramatically inhibited cell growth in conjunction with partial induction of differentiation in the CCRF-CEM, HL-60, and HGPRT ( - ) HL-60 …

Dietary And Hormonal Evaluation Of Men At Risk For Prostate Cancer, Donald J. Pusateri

Loma Linda University Electronic Theses, Dissertations & Projects

Relationships between dietary nutrients and plasma and fecal estrone, estradiol-17β, testosterone, and plasma prolactin levels were studied in young Seventh-day Adventist men: 18 nonvegetarians (NV), 20 lacto-ovovegetarians (LOV), and 15 vegans (V). Blood samples and 3 d dietary records were obtained. Contemporaneously collected diet composites and stool samples were analyzed for fiber. The vegetarians consumed significantly more fiber than the omnivores, while NVs and LOVs consumed more saturated fat than the Vs. Although plasma steroid hormone status did not differ, the Vs had significantly higher fecal estrogen levels than the NVs or LOVs. Plasma prolactin levels were significantly higher in …

Decreased Triiodothyronine Binding To The Hepatic Nuclear Thyroid Hormone Receptor In The Diabetic Mouse, Thomas J. Dewind

Loma Linda University Electronic Theses, Dissertations & Projects

The diabetic C57BL/KsJ-db m mouse has abnormal thyroid hormone levels and indications of thyroid hormone resistance. To investigate the basis of these abnormalities, the hepatic nuclear thyroid hormone receptor was extracted with 0.4 M KCl, 1.1 mM MgCl₂, 20 mM Tris/HCl, pH 7.9 from hepatocyte nuclei of normal C57BL/KsJ, heterozygous C57BL/KsJ-db m (db/m), and diabetic C57BL/KsJ-db m (db/db) mice. Normal and heterozygous mice were grouped together as the controls. Triiodothyronine (T₃) binding studies at 4°C using nitrocellulose filtration to separate free T₃ from receptor bound T₃ demonstrated an …

Insulin And Glucagon Responses To Dietary Protein In Diabetic And Nondiabetic Men, Monica L. Blomstedt

Loma Linda University Electronic Theses, Dissertations & Projects

Five men with non-insulin-dependent diabetes mellitus and fourteen nondiabetic men, were given test meals with soy protein, casein or additional carbohydrate in place of protein. Blood levels of insulin, glucagon, glucose and lipids were measured at fasting, one-half hour and two hours postprandial. To all meals, the nondiabetic subjects responded with a quick rise of the insulin level which decreased at two hours. In contrast, the diabetics showed a significantly lower insulin response at one-half hour (p < 0.05), but a tendency to higher levels at two hours. The same pattern was observed in the insulin to glucagon ratio. No significant difference between the meals was found in regard to insulin or glucagon responses, suggests that a sustained, although delayed postprandial insulin response, may be a precursor to the higher cholesterol and increased risk for cardiovascular disease among diabetics.

Sprague-Dawley Rats Fed Milk Or Beef Protein : Differences In Response To 1,2-Dimethylhydrazine Carcinogenesis, Stanley W. L. Ng

Loma Linda University Electronic Theses, Dissertations & Projects

Colon cancer is responsible for a high percentage of cancer deaths in developing countries, and there is convincing epidemiological evidence that meat protein and fat in the diet increase the incidence of this form of cancer. An animal tumor model which has been used by many investigators interested in colon cancer is the 1,2-dimethylhydrazine (DMH)-rat or DMH-mouse tumor model. In previous work done in this laboratory the DMH-BALB/c mouse tumor model was used to compare the production of colon tumors in mice fed a diet using non-fat powdered milk as a source of protein with those in mice fed beef …

An Overview Of The Newer Antibiotics, Ramon Del Busto

Henry Ford Hospital Medical Journal

The introduction of a large number of new antibiotics has made selecting the one most appropriate for treatment a confusing task for the practicing physician. One problem is that the differences in pharmacokinetics, in vitro activity, and clinical indications between some of these agents may be relatively minor; this is especially true of the new cephalosporins. Another problem is that the generic names of the cephalosporins are so similar that it is impractical, even for the infectious diseases specialist, to be familiar with all of them. This review attempts to summarize the most important characteristics of these new antibiotics and …

Lipoprotein Lipase Inhibitor Removal During Ultrafiltration Of Renal Failure Patients, Alisa Minear

Loma Linda University Electronic Theses, Dissertations & Projects

Plasma samples were obtained from seven hypertriglyceridemic (>150 mg/dl) chronic renal failure patients before and after regular hemodialysis. The effect of the plasma on the lipolytic activity of bovine milk lipoprotein lipase was investigated. The lipoprotein lipase activity, as measured by release of C¹⁴-labeled free fatty acid from triglyceride, was decreased 8-67% when incubated with uremic plasma as compared to the control plasma (44 mg/dl triglyceride). The inhibition noted prior to hemodialysis did not appear to be alleviated by the dialysis but subsequent treatment with an isolated ultrafiltration technique did appear to remove an inhibitory substance as …

In Vitro And Clinical Studies Of Cefaclor, A New Cephalosporin, Daria Kiani, Tom Madhavan, Keith Burch, Don Pohlod, Evelyn Fisher, Edward L. Quinn

Henry Ford Hospital Medical Journal

In vitro susceptibility studies of 246 clinical isolates demonstrated that this antibiotic was effective against Group A beta hemolytic streptococci, alpha hemolytic streptococci, S. pneumoniae, both penicillinase producing and nonpenicillinase producing Staphylococci, Klebsiella pneumoniae, and Proteus mirabilis. Cephalothin susceptible E. coii were also susceptible to cefaclor. Pseudomonas aeruginosa, Serratia sp., Enterobacter Sp., and Streptococcus faecalis were uniformly resistant to cefaclor. The efficacy and safety of this antibiotic were studied in 27 patients with urinary tract, soft tissue, and respiratory infections. Patients with urinary tract infections became abacteriuric after 48 hours. Patients with soft tissue infections responded well within the first …

Ua35/11 Student Honors Bulletin, Vol. Iv, Wku Honors Program

WKU Administration Documents

The articles, all papers done for classes in the university honors program, are divided into three groups. The first group deals with science, especially the area of health care, one of the more practical applications of science in the modern age. The second group consists of three papers done for honor courses in which the major purpose was to expose the student to new areas of consciousness and have them respond orally and verbally to what they read, creative writing. The last group deals with the humanities, literature and religion, two of the more popular fields of the humanities today. …

Amikacin: Clinical And Laboratory Studies, Erwin J. Haas, T. Madhavan, Evelyn J. Fisher, Frank Cox, Keith Burch, Edward L. Quinn, Richard D. Nichols

Henry Ford Hospital Medical Journal

Amikacin was evaluated in vitro against S aureus and a variety of gram negative bacilli. In concentrations of 12.5 μg/ml or less, it suppressed the growth of all organisms except 15% of Proteus mirabilis. Peak serum levels in patients, following a 7.5 mg/kg IM dose, averaged 21 μg/ml at one hour. Most of the drug appeared in the urine during the first six hours after administration. Of six patients receiving an adequate course of treatment, all but one were cured. Treatment in that patient also failed with a subsequent course of gentamicin and carbenicillin and he was cured only by …

Promethazine-Chlorpromazine Combination In The Treatment Of Unmanageable Psychotic Patients, Armando R. Favazza

Henry Ford Hospital Medical Journal

Administering a combination of promethazine and chlorpromazine to patients with a "galloping psychosis" has an antipsychotic and tranquilizing effect which calms them down to a more manageable and less aggressive state. The drugs are chemically similar; promethazine's actions are strongly potentiated in the combination, so large doses must be given under careful supervision. Case histories demonstrate successful short term management of acutely psychotic, aggressive patients who were a danger to themselves and others.

A Preliminary Evaluation Of Azathioprine (Imuran®) In The Treatment Of Multiple Sclerosis, William G. Tucker, K. H. Kapphahn

Henry Ford Hospital Medical Journal

A theoretical approach to the unknown etiology of multiple sclerosis is presented in this preliminary study which reports the results of empiric treatment with azathioprine. Imuran® therapy produced stabilization of the disease, neurological improvement, and no progress of neurological dysfunction or loss of any regained function. Evidence points toward a viral-induced immunological defect. Abnormal gamma globulins found in the cerebral spinal fluid suggest an immunological etiology.

Accidental Ingestion Of Anticoagulant Resulting In A Severe Hemorrhagic Diathesis, R. W. Monto, E. R. Williams, T. H. Greidanus, E. J. Van Slyck

Henry Ford Hospital Medical Journal

This case history of a 45-year-old woman, who had been issued Dicumarol instead of Dronactin in a pharmacy-filled prescription, describes an emergency therapeutic program to control hemorrhage with vitamin K, adrenal corticosteroids, and the giving of fresh blood. In a patient with a bleeding disorder, unintentional ingestion of oral anticoagulants is to be suspected if the prothrombin time is markedly elevated, if the patient has been previotisly treated with an oral anticoagulant, or is engaged in a medical or paramedical occupation. Diagnosis is confirmed by finding a deficiency of factors II, VII, IV, and X. and by the presence of …

Lsd - An Outsider's Viewpoint, E. J. Alexander

Henry Ford Hospital Medical Journal

No abstract provided.

Pharmacology Of Ophthalmologically Important Drugs, James L. Tucker

Henry Ford Hospital Medical Journal

No abstract provided.

A Study Of The Effect Of Varying Serum Phenylalanine Levels On Changes In Intelligence Quotient, Darlene Imai Manullang

Loma Linda University Electronic Theses, Dissertations & Projects

Data from thirty medical records of phenylketonuric children under treatment were investigated to determine at which serum phenylalanine level (0-3.9 mg. %, 4-7.9 mg %, 8-11.9 mg. %, greater than 12 mg. %) there would be the greatest increase in intelligence. The children involved in the study were divided into the following groups according to the age dietary treatment was initiated: 0 through 5 months, 6 through 11 months, 12 through 23 months, and over 2 years of age.

Among 6 patients who were placed on the diet during the first 5 months of life, it appears that the optimal …

Narcotic Drugs In The Postanesthetic Recovery Room, John W. Ditzler, Paul R. Dumke, Beverly Collier

Henry Ford Hospital Medical Journal

No abstract provided.

Clinical Evaluation Of Methyclothiazide, A New Potent Long-Acting Oral Diuretic Drug, Stephen Podolsky, Morton Fuchs, Robert Sellor

Henry Ford Hospital Medical Journal

No abstract provided.

The Use Of Phenothiazines On A General Hospital Psychiatric Service, Robert S. Schopbach

Henry Ford Hospital Medical Journal

No abstract provided.

A Review Of Drug Addiction And A Study Of Its Relation To Carbohydrate Metabolism, Eppie Chung Hartsuiker

Loma Linda University Electronic Theses, Dissertations & Projects

The specter of addiction eventually involves everyone of the community. It is a three-fold problem of socio-economic, psychological, and physiological factors. The least understood is the physiological or biochemical aspect of addiction.

It has been postulated that an upset in carbohydrate metabolism may be a biochemical cause of addiction. Several avenues were employed to investigate this possible cause of addiction.

1. A diet survey was conducted and revealed a greater consumption of refined carbohydrates, confections and alcoholic beverages in addicts.

2. Five-hour oral glucose tolerance tests were performed on 7 addicts and 12 non-addicts. These tests revealed that there were decreased glucose …

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A Review Of Psychopharmacologic Drugs, Robert R. Schopbach

Henry Ford Hospital Medical Journal

No abstract provided.

Hydrochlorothiazide In The Treatment Of Congestive Heart Failure, John W. Keyes, Gerald M. Breneman, Hernan Alvarez Jr.

Henry Ford Hospital Medical Journal

No abstract provided.

The Future Of Enzyme Research, Linus Pauling

Henry Ford Hospital Medical Journal

No abstract provided.

Clinical Evaluation Of Chlorpromazine Hydrochloride In Acute Nausea And Vomiting, L. A. Swinehart

Henry Ford Hospital Medical Journal

No abstract provided.

Intravenous Procaine During Thoracic Surgery, Forrest E. Leffingwell

Medical Arts and Sciences: A Scientific Journal of the College of Medical Evangelists

Surgery on the heart and paracardial structures or traction on the hilus of the lung may initiate serious arrhythmias which can result in death if not promptly controlled. Procedures involving the pericardium are particularly apt to be followed by severe derangements of rhythm. Thus anesthesiology is faced with another challenge.

It is now evident that the intravenous administration of procaine has become a valuable addition to the armamentarium of the anesthesiologist in his endeavor to preserve the integrity of the thread of life while the surgeon carries out his heroic assignment without restrictions.

A Resume Of Intravenous Procaine Therapy, Fred B. Moor

Medical Arts and Sciences: A Scientific Journal of the College of Medical Evangelists

Summary:

1. The intravenous injection of procaine hydrochloride for a variety of clinical conditions constitutes a surprising new chapter in therapeutics.

2. With adequate precautions as to dosage and rate of administration, procaine can be safely given by the intravenous route in concentrations of 0.1 to 1 per cent.

It has been used successfully for serum sickness, the pruritis of jaundice, trauma of soft tissues, bones and joints, in the prevention and treatment of cardiac arrhythmias during intrathoracic surgery, for the relief of pain in osteoarthritis, for the control of pain during the dressing of burns, for postoperative pain, and …

The Central Action Of Procaine, Clarence W. Olsen

Medical Arts and Sciences: A Scientific Journal of the College of Medical Evangelists

Procaine was introduced as a local anesthetic with advantage over cocaine, especially as regards relative freedom from dangerous central effects. It [now] appears that when procaine hydrochloride is injected intravenously, the indications of a central effect are more emphatic when the solution is given rather rapidly.

Probably some of the central effects of procaine can be obtained with local infiltration. This is, of course, generally admitted when untoward results occur, but it should also be considered in connection with the favorable effects. Macpherson recently expressed the idea that in some cases we are getting the beneficial effects of procaine injection …