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- 3T3 Cells (1)
- <p>Cell adhesion -- Molecular aspects</p> (1)
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Articles 1 - 2 of 2
Full-Text Articles in Biomedical Informatics
Phosphorylation Of Elongation Factor 1 And Ribosomal Protein S6 By Multipotential S6 Kinase And Insulin Stimulation Of Translational Elongation, Y W Chang, J A Traugh
Phosphorylation Of Elongation Factor 1 And Ribosomal Protein S6 By Multipotential S6 Kinase And Insulin Stimulation Of Translational Elongation, Y W Chang, J A Traugh
Faculty, Staff and Student Publications
Stimulation of protein synthesis in response to insulin is concomitant with increased phosphorylation of initiation factors 4B and 4G and ribosomal protein S6 (Morley, S. J., and Traugh, J. A. (1993) Biochimie 75, 985-989) and is due at least in part to multipotential S6 kinase. When elongation factor 1 (EF-1) from rabbit reticulocytes was examined as substrate for multipotential S6 kinase, up to 1 mol/mol of phosphate was incorporated into the alpha, beta, and delta subunits. Phosphorylation of EF-1 resulted in a 2-2. 6-fold stimulation of EF-1 activity, as measured by poly(U)-directed polyphenylalanine synthesis. The rate of elongation was also …
Dexamethasone Decreases Cell-Cell Adhesion In The 235-1 Pituitary Tumor Cell Line, Phillip Richard Spangler
Dexamethasone Decreases Cell-Cell Adhesion In The 235-1 Pituitary Tumor Cell Line, Phillip Richard Spangler
Theses, Dissertations and Capstones
Physical contacts between cells are known to influence a broad range of biological activities. Investigations into the regulation of prolactin gene expression have shown that cell behavior within the pituitary may also be affected by adhesive contacts. To better understand the mechanisms that regulate cell-cell adhesive contacts in these endocrine cells, we developed a procedure that quantitatively assays homotypic aggregation in the rat pituitary tumor cell line, 235-1. We observed that treatment of 235-1 cells with dexamethasone (Dex), a synthetic glucocorticoid, not only reduces prolactin production but also decreases the reformation of cell-cell contacts. In two independent experiments, control cells …