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Full-Text Articles in Biomedical Informatics
High Body Mass Index Changes Peri-Tumor Adipose Tissue Which In Turn Promotes Triple Negative Breast Cancer, Cora Elizabeth Miracle
High Body Mass Index Changes Peri-Tumor Adipose Tissue Which In Turn Promotes Triple Negative Breast Cancer, Cora Elizabeth Miracle
Theses, Dissertations and Capstones
Cancer is one of the leading causes of death worldwide, responsible for over half a million deaths each year. There are multiple risk factors associated with the development of cancer. Some of these risks include genetics, smoking, and most recently, obesity (Lewandowska et al., 2019) (De Pergola & Silvestris, 2013). Research has shown that obesity is linked to the promotion of fourteen different cancers, including aggressive triple negative breast cancer (TNBC). Patients that are obese are more likely to develop cancer (Park et al., 2014). In addition, if the patient is obese at the time of a cancer diagnosis, they …
Gut Microbial Metabolite Indole: A Stimulator Of Enteroendocrine Cell Differentiation Via Activation Of Aryl Hydrocarbon Receptor, James Hart
Theses, Dissertations and Capstones
Enteroendocrine cells (EECs) regulate energy balance and glucose homeostasis by releasing hormones in response to food intake. Dysregulated EEC differentiation is observed in obesity, while gut microbiota metabolites influence this process. Here, we investigated the role of indole, a biologically active gut microbial metabolite, in EEC differentiation through aryl hydrocarbon receptor (AhR) activation. Human intestinal organoids derived from jejunal mucosal biopsies were exposed to indole. Indole treatment significantly increased mRNA levels of chromogranin A, an EEC marker. The effect was reversed by an AhR antagonist, indicating AhR involvement. Indole also upregulated AhR target gene mRNA levels. These findings highlight the …
Dexamethasone Decreases Cell-Cell Adhesion In The 235-1 Pituitary Tumor Cell Line, Phillip Richard Spangler
Dexamethasone Decreases Cell-Cell Adhesion In The 235-1 Pituitary Tumor Cell Line, Phillip Richard Spangler
Theses, Dissertations and Capstones
Physical contacts between cells are known to influence a broad range of biological activities. Investigations into the regulation of prolactin gene expression have shown that cell behavior within the pituitary may also be affected by adhesive contacts. To better understand the mechanisms that regulate cell-cell adhesive contacts in these endocrine cells, we developed a procedure that quantitatively assays homotypic aggregation in the rat pituitary tumor cell line, 235-1. We observed that treatment of 235-1 cells with dexamethasone (Dex), a synthetic glucocorticoid, not only reduces prolactin production but also decreases the reformation of cell-cell contacts. In two independent experiments, control cells …