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The Immune Elimination Of Senescent Human Rbc, Edward Marshall Alderman

MUSC Theses and Dissertations

Human erythrocytes (RBC) from whole blood were separated according to their specific densities by centrifugation on a polyvinyl-pyrrolidine-coated colloidal silica matrix (Percoll) into four major subpopulations. By indirect immunofluorescence array, the most dense RBC subpopulation with specific density >1.110 g/ml (3-5% of total RBC), was positive for membrane-bound immunoglobulin; the remaining, less dense subpopulations were negative. IgG was present on 85-95%, IgM on 28-32%, and IgA on 15-20% of the RBC in the most dense subpopulation. When these immunoglobulins were radiolabeled in situ, eluted, and used in binding studies with autologous RBC fractions subjected to thermal and/or enzymatic treatment with …