Anatomy Commons^™

A Synaptic Nidogen: Developmental Regulation And Role Of Nidogen-2 At The Neuromuscular Junction, Michael A. Fox, Matthew Sp Ho, Neil Smyth, Joshua R. Sanes

Anatomy and Neurobiology Publications

Background

The skeletal neuromuscular junction is a useful model for elucidating mechanisms that regulate synaptogenesis. Developmentally important intercellular interactions at the neuromuscular junction are mediated by the synaptic portion of a basal lamina that completely ensheaths each muscle fiber. Basal laminas in general are composed of four main types of glycosylated proteins: laminins, collagens IV, heparan sulfate proteoglycans and nidogens (entactins). The portion of the muscle fiber basal lamina that passes between the motor nerve terminal and postsynaptic membrane has been shown to bear distinct isoforms of the first three of these. For laminins and collagens IV, the proteins are …

Arabidopsis Mrna Polyadenylation Machinery: Comprehensive Analysis Of Protein-Protein Interactions And Gene Expression Profiling, Arthur G. Hunt, Ruqiang Xu, Balasubrahmanyam Addepalli, Suryadevara Rao, Kevin P. Forbes, Lisa R. Meeks, Denghui Xing, Min Mo, Hongwei Zhao, Amrita Bandyopadhyay, Lavanya Dampanaboina, Amanda Marion, Carol Von Lanken, Qingshun Quinn Li

Anatomy and Neurobiology Publications

Background

The polyadenylation of mRNA is one of the critical processing steps during expression of almost all eukaryotic genes. It is tightly integrated with transcription, particularly its termination, as well as other RNA processing events, i.e. capping and splicing. The poly(A) tail protects the mRNA from unregulated degradation, and it is required for nuclear export and translation initiation. In recent years, it has been demonstrated that the polyadenylation process is also involved in the regulation of gene expression. The polyadenylation process requires two components, the cis-elements on the mRNA and a group of protein factors that recognize the cis …

Protocol: Streamline Cloning Of Genes Into Binary Vectors In Agrobacterium Via The Gateway® Topo Vector System, Ruqiang Xu, Qingshun Quinn Li

Anatomy and Neurobiology Publications

Background

In plant functional genomic studies, gene cloning into binary vectors for plant transformation is a routine procedure. Traditionally, gene cloning has relied on restriction enzyme digestion and ligation. In recent years, however, Gateway^® cloning technology (Invitrogen Co.) has developed a fast and reliable alternative cloning methodology which uses a phage recombination strategy. While many Gateway- compatible vectors are available, we frequently encounter problems in which antibiotic resistance genes for bacterial selection are the same between recombinant vectors. Under these conditions, it is difficult, if not sometimes impossible, to use antibiotic resistance in selecting the desired transformants. We have, …

Generation Of Recombinant Mouse Embryonic Stem Cell Lines And Theirapplication For In Vivo Bioluminiscence Imaging In The Heart, Ramana Kammili

Electronic Theses and Dissertations

Cardiovascular disease is the major cause of death in the United States, with 80 million people suffering from some form of heart disease each year. One major limitation is the inability of the heart to repair the damaged tissue. Stem cell therapy holds enormous promise to repair and regenerate the damaged myocardium, but there are many technical difficulties that must first be overcome. One such difficulty is the present lack of ability to track and assess transplanted stem cells over time in vivo. The central hypothesis of this thesis is that in vivo bioluminescence imaging is a safe and useful …

Making Sense Of Men's Workout Practices: The Body, Age And Identity, Andrew Dunne

Masters

Background: Within sociology it is generally accepted that the body has become an object or “project” that is worked on and transformed as a central part of self-identity (Baudrillard 1998; Corrigan 1997; Featherstone 1991; Giddens 1991; Turner 1995, 1992; Shilling 2003). An alternative to such arguments, Leder (1990) conceptualizes the body as an “absent presence”. He argues that, while the body plays a central role in shaping our experience of the world, we are frequently oblivious to our own bodies. For Leder, bodywork is sporadic. He contends that specific social and/or physiological experiences cause the body to “dys-appear”, or enter …

Department Of Anatomy And Cell Biology: 1983-2007, Garl K. Rieke

UND Departmental Histories

This departmental history was written on the occasion of the UND Quasquicentennial in 2008.

Resistance Training And Bone Mineral Density During Growth, M. Z. Smith, B. M. Goettsch, R. D. Van Ramshorst, J. A. O'Brien, S. V. Jaque, Ken D. Sumida

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

This study examined the efficacy of two different resistance training programs in enhancing bone modeling and bone mineral density (BMD) in maturating rats. One exercise mode involved lifting a lighter weight with more repetitions (LI), while the other regimen involved lifting a heavier weight with fewer repetitions (HI) where the total volume of work between exercise programs was equivalent by design. Twenty-three male rats were randomly divided into control (Con, n = 8), LI (n = 7), and HI (n = 8) groups. The LI and HI groups were conditioned to climb a vertical ladder with weights appended to their …

Parent Perceptions Of School-Based Support For Students With Traumatic Brain Injuries, Susan C. Davies, Shari L. Wade, Michelle Wu

Counselor Education and Human Services Faculty Publications

Primary objective: To determine whether parents believe schools provided necessary support to their children who sustained traumatic brain injuries.

Research design: Interview, to determine parent perceptions

Methods and procedure: Sixty-six primary caregivers of school-age children who experienced a TBI within the previous 2 years were interviewed regarding what types of special support were needed by and provided for their children during the 3 months immediately following school reentry. They then rated how difficult it was to obtain support or services from the school and how satisfied they were with the support or services.

Main outcomes and results …

One-Year Treatment With Mometasone Furoate In Chronic Obstructive Pulmonary Disease, Peter M.A. Calverley, Stephen I. Rennard, Harold S. Nelson, Jill P. Karpel, Eduardo H. Abbate, Paul Stryszak, Heribert Staudinger

Journal Articles: Pulmonary & Critical Care Med

Many patients with chronic obstructive pulmonary disease (COPD) are treated with twice daily (BID) inhaled corticosteroids (ICS). This study evaluated whether daily PM mometasone furoate administered via a dry powder inhaler (MF-DPI) was equally effective compared to twice daily dosing.In a 52-week, randomized, double-blind, placebo-controlled study, 911 subjects with moderate-to-severe COPD managed without ICS received MF-DPI 800 microg QD PM, MF-DPI 400 microg BID, or placebo. The change from baseline in postbronchodilator forced expiratory volume in 1 second (FEV1), total COPD symptom scores, and health status as well as the percentage of subjects with a COPD exacerbation were assessed. Adverse …

Interrupted Vs. Uninterrupted Training On Bmd During Growth, B. M. Goettsch, M. Z. Smith, J. A. O'Brien, G. V. Gomez, S. V. Jaque, Ken D. Sumida

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

This study compared a resistance training program where the exercise was uninterrupted (UT, i.e., continuous repetitions) against a resistance training program where the exercise was interrupted (IT, i.e., 3 exercise sessions during a training day) for enhancing bone modeling and bone mineral density (BMD) in maturating animals. The total volume of work performed between the two resistance training programs was equivalent by design. 24 young male rats were randomly divided into Control (Con, n = 8), UT (n = 8) and IT (n=8) resistance trained groups. The UT and IT groups were conditioned to climb a vertical ladder with weights …

Role Of Reactive Oxygen Species Peroxynitrite In Traumatic Spinal Cord Injury, Yiqin Xiong

University of Kentucky Doctoral Dissertations

Peroxynitrite (PN, ONOO-), formed by nitric oxide radical (^•NO) and superoxide radical (O2^•-), plays an important role in post-traumatic oxidative damage. In the early work, we determined the temporal characteristics of PN-derived oxidative damage in a rat spinal cord injury (SCI) model. Our results showed 3-nitrotyrosine (3-NT), a specific marker for PN, rapidly accumulated at early time points (1 hr, 3 hrs), after when it plateaued and the high level was sustained to 1 week post injury. The co-localization of 3-NT and lipid peroxidation derived-4-HNE observed in immunohistochemistry indicates PN is involved in lipid peroxidative as …

Continence Care: The Need For Creativity And Innovation, Karen A. Karlowicz

Ellmer School of Nursing Faculty Publications

(First paragraph) In April 2007, I attended a conference quite different from anything I had previously experienced. The conference, Innovating for Continence: The Engineering Challenge, was hosted by the Simon Foundation for Continence. As the title implies, this was not your usual conference about the diagnosis, treatment, and management of urinary incontinence. Rather, it was a conference dedicated to exploring technological advances in continence care. The central question throughout this two-and-a-half day event was, "What can be done to simulate continence technology to ensure the most effective management for persons who suffer with this problem?"

Effect Of Thyroid Hormones On At1r Mrna Levels In Hek 293 Cells: Implications For Early-Onset Hypertension In Children Born Of Preeclamptic Women, Amanda Eddins

Theses and Dissertations

This experiment tested the effect of thyroid hormones, T3 or T4, on angiotensin II type 1 receptor mRNA levels in human embryonic kidney cells (HEK). Maternal preeclampsia is a prediction of higher ambulatory blood pressure (ABP) in the exposed offspring. The mother's blood pressure affects the blood pressure of the fetus since the placenta mediates blood flow. Thyroid hormone can increase production of angiotensinogen, which is a part of the renin-angiotensin system (RAS). RAS regulates blood pressure. Hypothesis: HEK 293 cells treated with T3 and T4 will have more angiotensin II type I receptors than those not treated with T3 …

Effects Of Sepsis On Nerve Evoked Responses, Kevin Richard Novak

Browse all Theses and Dissertations

Sepsis and SIRS (systemic inflammatory response syndrome) have become two expensive and complicated problems seen in the intensive care unit (ICU). These two illnesses have been known to cause dysfunction with excitable tissues in the body. Encephalopathy, neuropathy, and myopathy are the three biggest. In this paper we discuss the development of an animal model of sepsis and the neurological complications sepsis brought about. Nerve conduction studies showed increased durations on compound muscle action potential, and decreased amplitude as well as increased duration and latency on sensory nerve action potentials. These results were not consistent with the two most common …

Oxidative Stress And Cell Death In Osmotically Swollen Glial Cells, Crystal Elaine Stuckey

Browse all Theses and Dissertations

Regulation of normal volume is an important aspect of cell homeostasis. Possible mechanisms which signal volume regulation are increasing the rate of reactive oxygen species (ROS) production and release of ATP for interaction with purinergic receptors. We examined whether an increase in ROS production during cell swelling also led to cell injury of C6 glioma cells. Cells were loaded with 5,6-carboxy-2,7-dihydrofluorscein diacetate (DCFDA) to fluoroscopically measure the rate of cellular ROS production and were perfused with phosphate-buffered saline solutions (PBS) containing 100 μM carbenoxolone to inhibit dye efflux. Cell death was determined cytometrically and by measuring the release of lactate …

Effects Of Subcutaneous Postnatal Choline Supplementation On Hippocampus-Mediated Learning And Memory In Rat Pups, Jeremy Alan Moore

Browse all Theses and Dissertations

The effects of postnatal injections of choline on the acquisition of two variants of eyeblink conditioning were examined in developing Long-Evans rat pups. Choline administration on postnatal days (PND) 15-27 was followed by evaluation of trace eyeblink conditioning (Experiment 1) and delay eyeblink conditioning (Experiment 2) on PND 28-29. The results of these experiments show that choline produced greater improvements in learning and memory during the trace condition than in the delay condition, presumably due to its effect on the hippocampus. Trace eyeblink conditioning relies heavily on an intact hippocampus but delay eyeblink conditioning does not, and it is understood …

Stress Reducing Effects Of Oxytocin In A Maternal Separation Paradigm, Keely Jane O'Connell

Browse all Theses and Dissertations

This study compared the effects of centrally and peripherally administered oxytocin (OT) on HPA axis activity and the presence of both stress-induced active and passive behaviors in female guinea pig pups (Cavia porcellus) after 180-min of isolation . In Experiment 1, one dose of oxytocin (10μg/.2ml) was injected subcutaneously into the periphery. Plasma cortisol levels were reduced after 180-min of isolation and two passive behaviors, eye-closure and crouch, were reduced as well with the administration of oxytocin. In Experiment 2, two different doses (10μg/.2ml and 20μg/.2ml) were injected into the periphery with no significant results. In Experiment 3 one dose …

Rule-Based Mamdani-Type Fuzzy Modeling Of Skin Permeability, Deepak R. Keshwani, David D. Jones, George E. Meyer, Rhonda M. Brand

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Two Mamdani type fuzzy models (three inputs–one output and two inputs–one output) were developed to predict the permeability of compounds through human skin. The models were derived from multiple data sources including laboratory data, published data bases, published statistical models, and expert opinion. The inputs to the model include information about the compound (molecular weight and octonal–H₂O partition coefficient) and the application temperature. One model included all three parameters as inputs and the other model only included information about the compound. The values for mole molecular weight ranged from 30 to 600 Da. The values for the log …

Three Dimensional Echocardiography In Congenital Heart Defects., Girish S. Shirali

Manuscripts, Articles, Book Chapters and Other Papers

Three dimensional echocardiography (3DE) is a new, rapidly evolving modality for cardiac imaging. Important technological advances have heralded an era where practical 3DE scanning is becoming a mainstream modality. We review the modes of 3DE that can be used. The literature has been reviewed for articles that examine the applicability of 3DE to congenital heart defects to visualize anatomy in a spectrum of defects ranging from atrioventricular septal defects to mitral valve abnormalities and Ebstein's anomaly. The use of 3DE color flow to obtain echocardiographic angiograms is illustrated. The state of the science in quantitating right and left ventricular volumetrics …

Dependence In Prestroke Mobility Predicts Adverse Outcomes Among Patients With Acute Ischemic Stroke, Mary I. Dallas, Shari Rone-Adams, John L. Echternach, Lawrence M. Bass, Dawn M. Bravata

Rehabilitation Sciences Faculty Publications

Background and Purpose - Stroke survivors are commonly dependent in activities of daily living; however, the relation between prestroke mobility impairment and poststroke outcomes is poorly understood. The primary objective of this study was to evaluate the association between prestroke mobility impairment and 4 poststroke outcomes. The secondary objective was to evaluate the association between prestroke mobility impairment and a plan for physical therapy.

Methods - This was a secondary analysis of the National Stroke Project data, a retrospective cohort of Medicare beneficiaries who were hospitalized with an acute ischemic stroke (1998 to 2001). Logistic-regression modeling was used to examine …

The Effects Of Atomoxetine On Cognitive Performace And Neuroplasticity After Traumatic Brain Injury, Wendy Reid

Theses and Dissertations

Catecholaminergic neurotransmission is regionally altered following injury, and drugs aimed at these systems offer promising avenues for post-TBI pharmacotherapies. Atomoxetine is a selective norepinephrine transporter (NET) inhibitor currently indicated for treatment of attention-deficit hyperactivity disorder (ADHD). The studies in this dissertation were designed to test the efficacy of atomoxetine for treating cognitive deficits following experimental TBI and the potential mechanism for any beneficial effect. The first part of the study focused on behavioral recovery following atomoxetine treatment. Several important questions of dose, therapeutic window, and duration of treatment were addressed in these studies. Sprague-Dawley rats were subjected to lateral fluid-percussion …