Anatomy Commons^™

A Semiquantitative Analysis Of The Chorda Tympani Taste Pathway In The Rat Brain With The 2-Deoxyglucose Method, Charles L. Hardison

Loma Linda University Electronic Theses, Dissertations & Projects

This thesis is based on a semiquantitative analysis of four nuclear relay centers [nucleus tractus solitarius (NTS), parabrachial nucleus (PBN), ventroposteromedial nucleus (VPM), gustatory neocortex (GNC)] in the chorda tympani nerve pathway. Metabolic changes were examined by the 2-deoxyglucose method in which one chorda tympani nerve was chemically stimulated and its contralateral side lesioned. Coronal sections of four taste centers were prepared for autoradiography and Nissl staining. Densities of the autoradiograms were determined by means of a video based image processor. Relative differences in the optical density ratios of the stimulated versus lesioned nuclear grey matter areas to white matter …

Age-Related Changes Of Neurites In Meissner Corpuscles Of Diabetic, Vicky R. Ras

Loma Linda University Electronic Theses, Dissertations & Projects

This thesis is based on a qualitative and quantitative study of the neural component of Meissner corpuscles of diabetic and non-diabetic mice. Age-related changes were also documented in these mechanoreceptors. Corpuscles from forty-six diabetic mice and forty-six non-diabetic mice ranging from 2.5 to 17 months old were studied. Forepaw index digital pads were formalin-fixed and frozen-sectioned parallel to each digit and perpendicular to the skin. Serial sections were then silver-impregnated to allow light microscopic examination of the neurites in the corpuscles.

Neurites became more coarse, tortuous, branched, varicose, and thus more complex until middle age. Corpuscle size appeared greatest at …

Distribution And Proportion Of Immunoreactive Neuron Specific Enoclase Containing Cells In The Rat Pineal Gland, Alfonso Miranda C.

Loma Linda University Electronic Theses, Dissertations & Projects

The distribution and proportion of cytoplasm containing immunoreactive neuron specific enolase (NSE) in the rat pineal have been investigated. Step serial sections of the pineal glands of 7 male Sprague-Dawley rats were stained for NSE using an indirect immunoperoxidase method. The volumes of the pineals and of the regions containing reactive cells were derived from the distance between sections and the sum of the corresponding sectional areas of camera lucida drawings. The proportion of the NSE areas actually consisting of NSE reactive cytoplasm was measured using an image array processor.

Cytoplasm reactive for NSE was consistently localized to the posterior …

Age-Changes Of The Neuronal Component Of Meissner Corpuscles In The Mouse Digital Pad, Roger C. Mathewson

Loma Linda University Electronic Theses, Dissertations & Projects

Silver impregnated sections of mouse digital pads were studied using light microscopy to detect age-related changes of the neuronal component of Meissner corpuscles. Direct microscopic observation, photomicrographs and camera lucida tracing were utilized. From qualitative observation, the corpuscular neurites were found to undergo morphological age-related changes of diameter, tortuosity, varicosity, branching and terminal expansion size. Quantitative examination was made of the number of corpuscles, corpuscular neurites, branching neurites, cross-innervations, terminal neurite expansions, neurite intraepidermal continuations and terminal axonal processes. The number of corpuscles and neurite intraepidermal continuations decreased with age while having significant linear correlation; whereas, branching increased with age …

The Neural Organization Of The Lamina Ganglionaris In The Crab Hemigrapsus Nudus A Light And Electron Microscope Study, Hsu-Cheng Huang

Loma Linda University Electronic Theses, Dissertations & Projects

The gross structure and the neural arrangements in the optic lamina of crab Hemigrapsus nudus have been studied by light microscopy Golgi techniques and by electron microscopy. The lamina from distal to proximal is composed of a distinct cell layer, a fibular synaptic region, the plexiform layer, a spares cell layer, and the first optic chiasma. Golgi preparation of the lamina reveal four types of cells contributing to the lamina synaptic layer. Retinula cell axons and terminations, monopolar neurons with perikarya in the distal cell layer, tangential cells, neurons whose primary process enters from the chiasma with secondary processes branching …

Cineradiography Of The Temporomandibular Joint : A Research Entity And Diagnostic Modality, L. Albert Lewis

Loma Linda University Electronic Theses, Dissertations & Projects

Readily accessible equipment as utilized for cineangiography has been demonstrated to be useful in producing dynamic studies of the temporomandibular joint.

The reproducible studies have been obtained without adaptation of equipment. Methods used in this project were based on principles which have been advocated for the past thirty years for static radiographs of the temporomandibular joints.

Studies were made of a variety of subjects varying in size and joint integrity. Diagnostic views were obtained on all subjects showing normal osseous joint structures and typical joint function via the use of motion picture records. Changes in osseous joint structures were readily …

The Coronary Distribution Zone : Mathematical Modelling Of The Left Ventricular Epicardial Surface For Area Calculation, Three-Dimensional Rotaton And Display, S. Matts E. Sjölander

Loma Linda University Electronic Theses, Dissertations & Projects

A computer model of the left ventricular (LV) epicardial surface was developed from Jang’s truncated ellipsoidal model, which has a minor to major axis ratio of 6:10. The model was applied in calculating the area of a segment of the myocardium, jeopardized by an impending occlusion of its associated artery. The endangered segment of the LV muscle was also visualized in different projections by rotations in three space and graphical displays simulating the three dimensions.

The area formula for the surface of the ideal model , a truncated prolate spheroid, was derived and used in producing maps for manual calculations …

A Light And Electron Microscopic Study Of Visual Neurons In The Medulla Externa Of The Crab Hemigrapsus Nudus, Yu Cheng Liao

Loma Linda University Electronic Theses, Dissertations & Projects

The decapod crustacean with a relatively well-defined visual system have contributed significantly to our understanding of visual neuron morphology and physiology. The general morphology of the optic lobes of several decapods were initially described by Parker (1897) and Hanstrom (1924). In the past few years, several light and electron microscope studies have been made on the lamina ganglionaris: the first optic ganglion, of the lobster (Hamori and Horridge, 1966a); the crayfish (Hafner, 1974; Nassel, 1976a), and the crab (Stowe et al., 1977; Rafuse et al., in preparation). As yet, no adequate description has been made of the visual neurons in …

Isolation And Chemico-Physiologic Characterization Of A Parotid Hormone Activity From Porcine Glands, Jean-Marc Tieche

Loma Linda University Electronic Theses, Dissertations & Projects

For the past decade, evidence has accumulated which suggests that the resistance of the teeth to dental decay is in part under systemic control. It was shown that the resistance is accomplished through the optimal operation of a dentinal fluid transport mechanism which is believed to be hormonally controlled by the hypothalamic-parotid gland endocrine axis. Although circumstantial evidence for such an endocrine function was strong, the isolation of a parotid hormone which stimulates the dentinal fluid transport mechanism remained to be accomplished.

Using modern biochemical techniques, a methodology was designed to isolate and purify the hormone from porcine parotid glands. …

Influence Of Denervation And Hemithyroidectomy On The Age Related Increase In The Number Of Calcitonin-Containing Cells In Mouse Thyroid, Nualnoi Wechbanjong

Loma Linda University Electronic Theses, Dissertations & Projects

It has been concluded from this study that nerve fibers in the superior laryngeal nerves have an influence on the development of C-cells in the mouse thyroid. This conclusion is based on the results of two types of investigation. The first is an analysis of the effect of age on the number of C-cells and the second is an investigation of the influence of denervation and hemithyroid-parathyroidectomy on the age related changes in C-cells numbers.

I. The distribution and c011centraticn of C-cells in mouse thyroid at various ages.

Serial 6 µm sections of thyroid glands from Swjss Webster mice at …

Cranio-Facial Skeletal Morphology Of Digit Suckers, John Eric Peterson Jr.

Loma Linda University Electronic Theses, Dissertations & Projects

The purpose of this paper was to investigate the cranio-facial skeletal morphology found in digit suckers.

The criteria used for selecting the 50 patients in this study were a history of digit sucking and an anterior dental open-bite. The sample consisted of Class I and Class II, Division 1 malocclusions, ages six through adult, for both sexes. If an individual was post— pubertal, they were classified as an adult. A Ricketts lateral cephalometric analysis of each patient was compared to standards of the same age. These differences were analyzed statistically with a Student t test and a one-way analysis of …

Immuno-Histochemical Method For Demonstration Of Lactic Dehydrogenase "M" Subunits In Rat Skeletal Muscle, Kidney And Peripheral Nerve, Luis M. Bustos-Arango

Loma Linda University Electronic Theses, Dissertations & Projects

Immunolocalization of lactic dehydrogenase (LDH) rrM'‘' sub units has been achieved using paraformaldehyde vapor phase fixation of freeze-dried sections. An antiserum to monkey LDH-M^ was found to cross react with rat LDH-M subunits. The fibers of the gastrocnemius and soleus muscles which are stained using the immuno-peroxidase procedure do not stain in the presence of 4 M urea in the conventional method. In kidney, the collecting tubules are the structures which stain most intensely with the immuno-peroxidase procedure and in peripheral nerve both the Schwann cells and axons stain. The nuclei of muscle fibers. kidney tubular cells and Schwann cells …

The Deoxythymidine Kinase-Deoxythymidylate Kinase Enzymes In Salmonella Potsdam And Escherichia Coli K12 Infected With Bacteriophage P3, James D. Kettering

Loma Linda University Electronic Theses, Dissertations & Projects

Metabolic alterations in bacterial hosts caused by virus infections have been studied for the last thirty years. The virulent T-even bacteriophages have been studied the most and have been shown to be responsible for the induction of a number of new, virus-specific enzymes that are necessary for successful phage growth. In addition, the activity of other host enzymes present prior to infection has been found to be increased by T-even infection. Temperate bacteriophages, being more difficult to work with than the virulent viruses, have not been studied as extensively. This is especially true for metabolic studies, since most temperate phage …

The Developmental Fine Structure Of Pacinian Corpuscles, Pedro B. Nava

Loma Linda University Electronic Theses, Dissertations & Projects

A preliminary light microscopic study of the mouse hind limb revealed a group of about 20 Pacinian corpuscles extending from the mid-region of the fibula to the tibio-fibular fusion strategically located for the possible sensing of bone-transmitted vibration.

The fine structure of the corpuscles is described in four stages: primordial or prenatal (19 and 20 days); early differentiation (1-8 days postnatal); differentiated corpuscle (9, 10, 12, 14 and 21 days postnatal); and adult (28 days old).

The corpuscles commence development during the 19th prenatal day. The corpuscle then consists of a nerve ending surrounded by sheath-cells which appear to be …

A Computerized Roentgenographic Cephalometric Study Of Hydrocephalus, Charles R. Henry Jr.

Loma Linda University Electronic Theses, Dissertations & Projects

A preliminary roentgenographic cephalometric investigation of twelve hydrocephalics has been conducted to consider the effects of increased intracranial pressure and enlarged neural mass on the craniofacial complex.

The Ricketts computerized analysis, utilizing both posterior-anterior and lateral cephalograms, and the Steiner and Tweed analyses, utilizing the lateral cephalogram, were used to compare the hydrocephalic to the norm.

The anterior cranial base was found to be significantly increased in length possibly due to the effects of hydrocephalus. The effects of hydrocephalus on the facial measurements were questionable in view of the fact that the significant findings of: 1) excessive inclination and protrusion …

Kinesiology Of Knee Flexion Deformity Using Electromyography, Paul J. Roby

Loma Linda University Electronic Theses, Dissertations & Projects

A kinesiological electromyographic study was completed to determine the relative changes in muscle activity as a person walks and stands with different degrees of knee flexion deformity. The ultimate aim was to establish criteria for appropriate preventive and corrective measures relating to knee flexion deformity.

Six different muscles were implanted with wire electrodes. The subjects performed a series of walking and standing experiments with the knee flexed at selected angles by a locking leg brace to simulate knee flexion deformity. The electromyographic action potentials were monitored by analog tapes, oscilloscopes and closed circuit television. The analog tapes were displayed on …

A Study Of The Temporomandibular Joint Recording Center Concept, Edwin F. Shryock

Loma Linda University Electronic Theses, Dissertations & Projects

As the science of dentistry advances, more emphasis is being given to the function of the mandible. The recording center concept provides for the service of mandibular analysis, terminal hinge location, and mandibular recordings. This study is concerned with operating a mandibular recording center where patients can be referred for these specific services.

The practicability of a recording center has been determined by this study.

In order to assure accuracy and to reproduce consistent results, it seems necessary that the operators of recording centers be trained specifically for this purpose.

The complete process relies on the accurate location of the …

Physiological Parameters During Perfusion Fixation Of The Rat Central Nervous System For Electron Microscopy, Everet W. Witzel

Loma Linda University Electronic Theses, Dissertations & Projects

In an attempt to elucidate the problem of extracellular space within the cerebral cortex of the rat, several physiological parameters were analyzed during perfusion fixation with glutaraldehyde and acrolein for electron microscopy. These parameters were electroencephalography, electromyography, electrocardiography, cerebrospinal fluid pressure, impedance of cerebral cortex, perfusion pressure, and flow rate of the perfusate.

The first portion of this investigation dealt with the problem of asphyxiation of tissues during fixation. A substituted blood containing fluorocarbon which was oxygenated served as the means to study this problem. The last portion of this investigation dealt with buffers and molecular size in relationship to …

Selected Factors Related To Ability To Regain Bladder Function Following Indwelling Catheterization, Jongkolnee Padmasiri

Loma Linda University Electronic Theses, Dissertations & Projects

This exploratory retrospective study was designed to evaluate selected factors, including the ability to regain bladder function related to post indwelling urethral catheterization in an acute general hospital population.

The records of 107 patients who met the study criteria became the study population. These patients had three-way urethral catheters, continuous antibacterial irrigation and closed drainage system. Twenty patients died (19 percent) before the catheter was removed. Of the 87 patients who were alive, 37 percent (32 patients) had in-adequate bladder function. Of these 32 patients, no attempt was made to remove the catheter from 15 prior to their hospital discharge. …

Some Physiological Effects Of The Bed Bath On The Cardiovascular System Of Acute Myocardial Infarction Patients, Arleen Canfield

Loma Linda University Electronic Theses, Dissertations & Projects

At Loma Linda University, a protocol has been in effect that specifies the kinds and amounts of activity that acute myocardial infarction patients may have. The protocol has described six activity levels with each successive level permitting more activity. The patient must have reached the fourth level of progression before he is permitted to receive a complete bed bath. The problem studied in this research: As the result of receiving a bed bath, what physiological effects will occur in the cardiovascular system of patients with acute myocardial infarction?

The theoretical framework for this study consisted of the following: recommendations for …

An Ultrastructural Analysis Of Rat Posterior Pituitary With Special Reference To Hormone Release, William Mead Hooker

Loma Linda University Electronic Theses, Dissertations & Projects

[Abstract Not Included]

Mandibular Nerve Regeneration Through Solid And Canalized Bone And Plaster Grafts, Lawrence D. Day

Loma Linda University Electronic Theses, Dissertations & Projects

Utilizing an extra-oral approach and sterile technique, left mandibular ostectomy was performed on five miniature pigs. The segment removed was 3 cm long and included the inferior border, mandibular canal and, in some cases, apices of a molar tooth.

Bone and plaster grafts (one solid and one canalized of each) were used to fill these defects. The fifth was left with no graft.

The objective was to learn the effect of each of these procedures on regeneration of the mandibular nerve across the defect. It was hypothesized that canals through the grafts would facilitate regeneration.

X-rays were taken periodically. The …

A Cephalometric, Anatomic And Histologic Investigation Of The Effects Resulting From A Heavy Interrupted Orthopedic Force To The Maxilla Of The Macaca Mulatta Monkey, Roger Wayne Triftshauser

Loma Linda University Electronic Theses, Dissertations & Projects

The purpose of this study was to determine changes that occur in the craniofacial complex, when a heavy interrupted orthopedic force is employed to retract the maxilla in the post puberal rhesus monkey. Cephalometric, histologic and anatomic examinations were utilized to substantiate the findings.

Four female rhesus monkeys were used for experimentation. Two were experimental animals and the other two controls. Cervical retraction with headgear was applied over a period of 313 days and 159 days to the two experimental animals. Forces not exceeding 2.5 lbs. were found most effective.

The findings were as follows:-

1. A total posterior displacement of …

Some Aspects Of Cranial Bone Growth In The Puberal Macaca Mulatta Monkey: As Evidenced By Metallic Implants And Cephalometric Roentgenography And The Application Of A Geological Lapidary Technique For The Semi-Serial Thin Sectioning Of Undecalcified Bone, Lee Eugene Olsen

Loma Linda University Electronic Theses, Dissertations & Projects

The purpose of this study was to investigate cranial and facial bone growth in the puberal Macaca mulatta monkey over a six month period utilizing the methods of metallic implantation combined with serial cephalometric roentgenography and a bichromatic vital staining method.

The employment of the vital stains, alizarin Red S and anthracene blue, over the six month period of the study, failed to stain the actively growing bone as evidenced by gross and microscopic analysis of the undecalcified bone preparations.

Interimplant measurements were made across certain bone sutures and the following results were obtained: The zygomaticotemporal sutures showed marked growth …

Masseter Muscle Position Relative To Skeletal Open Bite And Skeletal Closed Bite, Alonzo David Proctor

Loma Linda University Electronic Theses, Dissertations & Projects

Modification of cephalometric roentgenographic procedures provided a means to identify the anterior borders of the right and left masseter muscles and relate them to surrounding dentoskeletal elements. Attachment of short, stiff, wires to the cheeks in a position superficial to the anterior borders of the muscles, subsequent to palpation and prior to exposure of the roentgenographic film, was the procedure used. The bi-lateral images projected on the film were averaged and traced along with dentoskeletal elements including those associated with skeletal type. This line representing the anterior border of the musculature was used to assess muscle position in the vertical …

A Cephalometric Study Of The Face And Dentition Of Several Contemporary Western United States And Alaskan Eskimo Groups, Robert C. Parkard

Loma Linda University Electronic Theses, Dissertations & Projects

A total of 223 cephalometric radiographs selected from six contemporary western United States and Alaskans native groups were analyzed by use of the Bjork cephalometric analysis. Those native groups selected were the Eskimos, Apaches, Navajos, Papagos, Pimas and Mayos. Approximately thirty to forty natives from each group were used in the study with ages ranging from thirteen to thirty-five years. The majority were in the fifteen to twenty year age range.

All data was submitted to various statistical analyses to establish a mean, standard deviation, and other relevant values for each of the native groups and to compare these values …

Influence Of Fluoride On Strength And Elasticity Of Femurs Of Rats Maintained On Diets Both Adequate And Low In Calcium, Dexter F. Beary

Loma Linda University Electronic Theses, Dissertations & Projects

The effect of fluoride on the physical properties of the rat femur was investigated by using an Instron materials tester. Experimental animals consisting of 80 female weanling rats were employed. These were divided into two major groups of 40 rats each. Group I received adequate calcium in the diet and Group II received a low calcium (0.1%) diet. Group I and II were divided into five subgroups, the first subgroup being used as the control. Each of the other four subgroups was given a different dosage level of fluoride in their drinking water as follows: the second subgroup received Hereford, …

An Odontometric Study Of Five Contemporary Western United States Indian And Alaskan Eskimo Groups, John A. Matis

Loma Linda University Electronic Theses, Dissertations & Projects

The morphologic characteristics of the oral cavity in five contemporary Western Indian and Alaskan Eskimo groups have been investigated by the use of study models of the dental archs. The sample consisted of 216 individuals, including 44 Navajos, 43 Apaches, 40 Papagos, 42 Pimas and 47 Alaskan Eskimos.

The first purpose of the study was to determine the extent of investigator error. This was accomplished by remeasuring many morphologic traits of the dentition in 125 Indians which had previously been measured by different investigators using the same study models. It was found that investigator error can be significant, even in …

Antibiotics And The Wasting Disease In Neonatally Thymectomized Rats, Douglas M. Grignon

Loma Linda University Electronic Theses, Dissertations & Projects

The administration of the antibiotic, Chloramphenicol, to pregnant rats just prior to, and for four days after delivery, diminishes the incidence of the so-called "Wasting Disease" in neonatally thymectomized rats.

A total of 50 rats were divided into the following groups:

1. Thymectomy only---nonmedicated 17 rats
2. Thymectomy only---medicated 12 rats
3. Thymectomy-adrenalectomy---nonmedicated 5 rats
4. Thymectomy-adrenalectomy---medicated 8 rats
5. Controls---nonmedicated 4 rats
6. Controls---medicated 4 rats

Chloramphenicol was administered in the drinking water to the mothers of groups 2, 4 and 6 for about 2 days prior to delivery and 4 days after. On the day of birth or within three days after birth, the …

An Analysis Of Orthodontic Treatment Results, William S. Asano

Loma Linda University Electronic Theses, Dissertations & Projects

A study of orthodontic treatment results was made on forty patients treated by the graduate students Loma Linda University. The purpose of the study was to determine the treatment changes in patients treated in the graduate clinic. The study was based on the findings of the cephalometric and full mouth radiographs. The distribution of the malocclusions were similar to those found in other studies. The group of forty patients was divided for paired comparisons of type of malocclusion, length of treatment, and extraction of teeth. Statistical correlations were computed on every change investigated in this study.

The Steiner Analysis and …