Medicine and Health Sciences Commons^™

The Effect Of A 12-Week Breathwork And Visualization Intervention On Inhibitory Control And Cognitive Flexibility In Adolescent Soccer Players, Louis Hein, Jace Nicolet, Tiffany Gomez, Mei-Jing Ang, Sven Hoekstra, Kelvin C. Wu

International Journal of Exercise Science: Conference Proceedings

Cognitive inhibition and flexibility are essential for soccer performance, enabling players to adapt to dynamic environments. However, accumulated season fatigue often degrades these executive functions. The Neurovisceral Integration Model posits that prefrontal cognitive function is linked to cardiac vagal tone, suggesting that autonomic regulation may support inhibitory control. While breathwork is known to enhance autonomic regulation, its potential to preserve cognitive function in youth athletes is untested. PURPOSE: This study investigated the effects of a 12-week breathwork and visualization protocol on inhibitory control (Stroop) and cognitive flexibility (Task-Switching) in adolescent soccer players. METHODS: Athletes were recruited from Soccer Central San …

Effect Of Acute Sleep Fragmentation On Skeletal Muscle Blood Flow During Handgrip Exercise, Gabriel Narvaez, Joaquin Gonzales

International Journal of Exercise Science: Conference Proceedings

Sleep fragmentation (arousals from sleep) has been shown to impair vascular function due to elevated inflammation and oxidative stress. Previous research on this topic has focused on resting vascular function, with little information available about the effect of poor sleep on hyperemic responses to exercise. PURPOSE: To test the hypothesis that acute sleep fragmentation impairs skeletal muscle blood flow during exercise. METHODS: Fifteen adults (7F, 8M, age- 25 ± 5 y, BMI 23.5 ± 2.5 kg/m2) participated in a randomized crossover study that included one night of habitual sleep and one night of sleep fragmentation separated by at least 7 …

The Effect Of Mental Imagery On Free Throw Shooting Percentage, Kaydon Jackson, Kadyn Hall, Casi Helbig

International Journal of Exercise Science: Conference Proceedings

Mental imagery is a cognitive process involving the internal simulation of sensory experiences without external stimuli and is commonly used in sport performance preparation. When structured and deliberately practiced, mental imagery can enhance skill acquisition and performance outcomes PURPOSE: This study investigated whether PETTLEP-guided mental imagery (Physical, Environment, Task, Timing, Learning, Emotion, Perspective), performed without any physical practice, could enhance free-throw accuracy. METHODS: Twenty collegiate baseball players were randomly assigned to a PETTLEP imagery group (n=10) or a control group (n=10). Over two weeks, the experimental group participated in five guided mental imagery sessions, each lasting five minutes, while the …

What The Dose?: Assessing The Effect Of Different Doses Of Arugula Extract Capsules On Blood Pressure And Endothelial Function, Autumn E. Andrews, Leroy K. Bolden, Andrew R. Gallucci, Tomas J. Chapman-Lopez, Mary C. Pickler, Olivia C. Russell, Katherine M. Lee, Savannah V. Rauschendorfer

International Journal of Exercise Science: Conference Proceedings

The effects of consuming nitrate-rich crops and supplements such as beetroot juice and spinach on endothelial function and blood pressure (BP) via nitric oxide (NO) production have been well established in previous literature. However, limited literature exists on arugula supplementation (e.g., arugula extract) as a source of NO and its effect on vascular function.  PURPOSE: The present study assessed the effect of high and low doses of arugula extract (AE) on blood pressure, vascular measures, and nitrate/nitrite concentrations. METHODS: Eight apparently healthy participants completed a single-blind crossover design study. All participants were randomized for three conditions (3000mg, 6000mg, …

Under Pressure: Arugula Extract’S Effect On Cardiovascular Measures In Hypertensive Individuals, Leroy K. Bolden, Andrew R. Gallucci, Autumn E. Andrews, Mary C. Pickler, Blake E. Wright, Tomas J. Chapman-Lopez, Savannah V. Rauschendorfer

International Journal of Exercise Science: Conference Proceedings

Hypertension (HTN) is one of the major contributing factors associated with the progression and development of cardiovascular disease (CVD). Moreover, HTN is expected to significantly increase by the year 2050. Nitrate supplements such as beetroot juice (BRJ) have been utilized as a dietary therapeutic to mitigate the progression of HTN and CVD; however, arugula extract, which has a higher nitrate content, has yet to be assessed. PURPOSE: The purpose of this study was to assess the effects of arugula extract on cardiovascular health measures in hypertensive individuals.  METHODS: 12 participants (M = 11; F = 1) with stage …

Mental Effort Responses To Low Cognitive Load Stimuli During Euhydrated Versus Hypohydrated Fatigue State, Austin Dunn, Lily Hargrove, James Rowe, Luis Aguerrevere, Eric Jones

International Journal of Exercise Science: Conference Proceedings

Improper body water regulation contributes to physiological changes, affects mood states, and impacts regulation of behavioral responses. With improvements in eye tracking technology (fixation duration and pupil diameter), the ability to quantify focal points and mental effort has expanded greatly. PURPOSE: To examine the effects that fatigue states and hydration, (euhydrated (Eu) vs. hypohydrated (Hypo)), has on average fixation duration and pupil diameter using five separate low cognitive load hydration stimuli. METHODS: Six college aged males underwent two trials of active fatigue/dehydration in an environmental chamber at 35℃ until 2% body water loss was achieved. Fluid restriction and ad-libitum consumption …

Do Different Practice Approaches Affect Intrinsic Motivation Based On A Goal-Directed Behavior?, Elissa D. Torres-Colon, Anthony W. Fava, Emily J. Sauers

International Journal of Exercise Science: Conference Proceedings

Optimizing motivation leads to enhanced motor performance and improved psychological health in human performers. However, the influence of the type of practice approach on intrinsic motivation among softball pitchers when they learn new pitches is less clear. PURPOSE: To compare intrinsic motivation in adolescent softball pitchers following a practice session using two different practice approaches. METHODS: Fifty adolescent-aged softball pitchers seeking to obtain a breaking pitch (drop ball) were randomly assigned into two practice designs, each with the same goal-directed behavior of “creating as much downward vertical break on the ball as possible.” One group practiced using a …

Sex Differences In Pre-Cooling Effect On Cognitive Processing Changes During Exercise In The Heat, Kristin N. Grabow, Amber M. Shipherd, Meral N. Culver, Christopher M. Hearon

International Journal of Exercise Science: Conference Proceedings

Cognitive processing (CP) declines during exercise are greater in the heat, but precooling (PC) prior to exercise in the heat can attenuate this decline. While males (M) and females (F) do not differ with regard to the impact of PC on thermoregulatory variables during exercise in the heat, F do demonstrate better perceptional values than M when using PC prior to exercise in the heat. Thus, if perceptional values potentially impact CP during exercise in the heat, then the PC impact on that response could differ between M and F. PURPOSE: The purpose of this study was to compare sex …

The Effects Of Multiple Circuit Training Sessions In Hot Compared To Thermoneutral Environmental Conditions On Environmental Symptomology, Jenna King, Ryan Dunn, Alexandra Brojanac, Mario Hernandez, Yasuki Sekiguchi

International Journal of Exercise Science: Conference Proceedings

Heat acclimation (HA) protocols have been under constant development since the 20th century, with the optimal HA approach yet to be determined. Adaptations from traditional approaches likely favor aerobic performance due to the nature of low- to moderate-intensity, endurance-based exercise in the heat. However, individuals involved in team sports, military personnel, and occupational workers may benefit from adaptations induced by a HA protocol that is more sport-/activity-specific. PURPOSE: To investigate differences in environmental symptomatology during a circuit training session in the heat (35°C; 40% relative humidity [RH]) following short-term (5 day) circuit training in hot and thermoneutral environmental conditions. METHODS: …

Effects Of Age And Sex On Reaction Time In Masters Track And Field Athletes, Yanbing Zhou, Natalie Mclaurin, Jonas Boecker, Phil Chilibeck, Tanja Taivassalo, Sean Forbes, Dominik Pesta, Jörn Rittweger, Hirofumi Tanaka

International Journal of Exercise Science: Conference Proceedings

Reaction time (RT) is a key indicator of cognitive processing speed and plays a critical role in athletic performance.  Investigating RT in masters athletes can provide valuable insight as it may help disentangle age-related cognitive processing from physical decline.  However, it is unknown whether masters athletes exhibit age-related declines in RT and whether sex and task complexity modulate these changes.  PURPOSE: To evaluate RT in male and female masters track-and-field athletes varying in age.   METHODS: One hundred and eighty-two masters athletes were recruited from the 2025 World Masters Athletics Indoor Track and Field Championships in Florida.  Four RT …

Do Body Composition And Cardiovascular Fitness Differ By Gender In Firefighters?, Giuliet L. Kibler, Kelly Hines, Michael J. Conner, Lisa C. Colvin, Steven E. Martin, Drew E. Gonzalez

International Journal of Exercise Science: Conference Proceedings

Firefighters (FF) endure substantial cardiovascular stress during their occupational tasks; yet, males and females differ in cardiovascular fitness (CRF) due to biological factors. The differences in body composition (BC) and CRF among FFs remain unclear. PURPOSE: To compare gender-specific outcomes in body BC and cardiopulmonary exercise test (CPET) of FF. METHODS: BC assessments via BIA and CPET on a cycle ergometer for 509 FF were analyzed from annual clinical testing. Gender was self-reported as follows: female (n = 29), male (n = 476), or other (n = 4). BC metrics, including body fat, lean body mass, skeletal muscle …

How Do Firefighters Differ Between Years Of Fire Service Experience: Part 1 - Physical Fitness Profiles, Ian Bivins, Michael J. Conner, Jacob A. Mota, Payton Miller, Steven E. Martin, Lisa C. Colvin, Drew Gonzalez

International Journal of Exercise Science: Conference Proceedings

Firefighters (FF) face a higher risk of cardiovascular disease (CVD) due to aging, job-related stress, and irregular physical activity. Years of experience (YXP) in the fire service have been shown to have a unique impact on FF health and performance; however, profiling the differences between those with less or more YXP has not yet been assessed. PURPOSE: To examine years of experience-related differences in conventional physical fitness parameters among FFs. METHODS: Archival data from annual clinical testing of 142 FFs were analyzed. Groups were defined as those with 5 YXP. General linear models (GLM) were used for multivariate …

Evaluating Self-Reported Dietary Habits And Barriers Among Rotc Cadets Across Body Mass Index Classifications, Sarah E. Johnson, Sheyla Leon, Michael Conner, Lisa Colvin, Steven Martin, Stella Volpe, Drew Gonzalez

International Journal of Exercise Science: Conference Proceedings

Limited data exist on the overall and sports nutrition knowledge among Reserve Officer Training Corps (ROTC) cadets across different body mass index (BMI) categories. PURPOSE: We aimed to study ROTC cadets' dietary habits and barriers across BMI groups. METHODS: Cross-sectional data were obtained from 184 ROTC cadets regarding validated questionnaires, such as the Perceived Barriers to Healthy Eating (PBHE), Food Choice Questionnaire (FCQ), and Rapid Eating Assessment for Participants-Short Version (REAPS). The cadets were stratified into body mass index (BMI) groups: underweight (RESULTS: Chi-square analyses showed BMI-related differences in responses to REAPS' breakfast skipping question (χ²=14.289, p=0.027, V=0.199), …

Evaluation Of Bone Turnover Markers And Biochemical Analysis In Developmental Metabolic Bone Diseases In Pediatric Aksaray Malakli Breed Dogs, Nihan Avci, Fehmiye Gumus Ozdil, Basak Boztok Ozgermen

The Thai Journal of Veterinary Medicine

Pediatric dogs are predisposed to skeletal disorders during growth, with rickets, hypertrophic osteopathy, and nutritional secondary hyperparathyroidism being the most common. Rickets, usually caused by vitamin D deficiency, leads to bone softening and deformities, whereas hypertrophic osteopathy involves abnormal calcium salt deposition that impairs mobility. This study investigated developmental orthopedic diseases in Aksaray Malakli dogs, focusing on nutritional factors and biochemical markers. Twenty-two pediatric dogs were examined and divided into two groups. Group I (n=11) showed gait abnormalities and postural deformities, whereas Group II (n=11) included clinically healthy dogs. Clinical, radiographic, and biochemical assessments were performed, including cortical index, parathormone, …

Quality Improvement Project: Leveling Pharmacology: A Faculty Development Initiative, Allison Berg

Doctor of Nursing Practice Projects

The purpose of this Doctor of Nursing Practice (DNP) quality improvement project was to address limited faculty self-efficacy in developing higher-level, competency-based pharmacology questions for clinical quizzes within an Associate Degree in Nursing (ADN) program. A faculty needs assessment identified an overreliance on recall-based pharmacology assessments, contributing to gaps in students’ clinical reasoning and medication competency. Guided by Bandura’s Theory of Self-Efficacy and Lewin’s Change Theory, a structured faculty development session was implemented to improve faculty confidence and skills in writing application-based pharmacology questions. A pre- post intervention design was used with ADN faculty participants (N = 5) at a …

Strain-Level Heterogeneity In Gardnerella Urinary Tract Persistence And Pathogenesis In An In Vivo Model Is Consistent With Comparative Phylogenomic Analyses, Lokesh Kumar, Sonia N Whang, Deborah K Verges, Robert F Potter, Nicole M Gilbert

2020-Current year OA Pubs

BACKGROUND: Gardnerella is a bacterial genera commonly present in the female urogenital tract, especially in bacterial vaginosis. Gardnerella are frequently detected in urine collected directly from the bladder and have been implicated in urinary incontinence and recurrent urinary tract infections. Understanding the contribution of Gardnerella to pathogenesis is complicated by its genetic heterogeneity and a shortage of data from in vivo models. One clinical isolate of Gardnerella triggered urothelial exfoliation and promoted urinary tract infection by uropathogenic E. coli in a mouse model. This study was designed to compare diverse Gardnerella strains in vivo to identify genomic variation associated with …

Adalimumab Therapy For Crohn’S Disease And Axial Spondyloarthritis In Latent Tuberculosis: A Bibliometric-Systematic Literature Review, Nyimas Maida Shofa, Nurike Setiyari Mudjari, Rusdiyana Ekawati, Arianti Arianti, Annisa Zahra Mufida, Budi Widodo, Titong Sugihartono

Sultan Qaboos University Medical Journal

The management of Crohn’s disease (CD) using anti-tumour necrosis factor therapy in tuberculosis (TB)-endemic regions poses major clinical challenges due to the risk of latent TB infection reactivation. This bibliometric–systematic literature review synthesised evidence from 17 peer-reviewed studies (2020–2025) evaluating adalimumab administered with concurrent prophylactic antitubercular therapy. Thematic synthesis and bibliometric mapping using VOSviewer demonstrated that adalimumab achieved 60–85% clinical remission in moderate-to-severe CD and axial spondyloarthritis while enhancing mucosal healing. However, 1–3% of patients developed active TB despite appropriate screening and isoniazid prophylaxis, revealing false-negative diagnostics and partial chemoprophylaxis protection. These findings highlight the need for region-specific frameworks integrating …

Advances In Hpv Vaccination In People Living With Hiv: A Review, Megan Mooberry, J Brooks Jackson, Mary B Rysavy

Faculty, Staff and Student Publications

Human papillomavirus (HPV) is the most common sexually transmitted infection worldwide and is a leading cause of cervical, anal, penile, and oropharyngeal cancers. This review summarizes the epidemiology of HPV and the immunogenicity, clinical efficacy, and current HPV vaccination recommendations among people living with HIV (PLWH). PLWH experience a disproportionate burden of HPV-related infection and HPV-related malignancies. Although HPV vaccines have been shown to be highly effective, vaccination coverage among PLWH remains suboptimal, particularly in low- and middle-income countries. Barriers to vaccination include extended dosing schedules, limited awareness of the vaccine, and misinformation. Evidence indicates HPV vaccines are safe and …

Airway Secretory Cells Contain Both A Perinuclear Golgi Ribbon And Dispersed Golgi Satellites, Oanh N Hoang, Colin E Chan, Joshua M Brenner, Denisse Leza-Rincon, Ana M Jaramillo, Brendan Dolan, Adam W Aziz, Rodolfo A Cardenas, Gerardo J Cardenas, Eduardo D Galvez, Joshua B Hales, Emilia S Nunez-Pena, Boxuan Yang, Reid T Powell, Leoncio Vergara, Harry Karmouty-Quintana, Jesper M Magnusson, Gunnar C Hansson, Roberto Adachi, John D Dickinson, Christopher M Evans, Justin A Courson, Alan R Burns, Michael J Tuvim, Burton F Dickey

Faculty, Staff and Student Publications

Rationale: Finely tuned production and secretion of the polymeric mucins MUC5AC and MUCB are required for lung health, but knowledge of many details between their translation and their packaging into secretory granules is lacking.

Objective: To analyze the structure and function of the Golgi apparatus, a key site of mucin glycosylation, folding, polymerization and packaging, in airway epithelial secretory cells.

Methods: Lung tissue was obtained from mice stimulated or not with IL-13 to upregulate mucin production, and from normal human lungs. Golgi elements in mouse and human tissue were imaged by high-resolution immunofluorescence microscopy and electron microscopy. Tissue from mice …

Plasma Ca125 Levels As A Predictor Of Major Adverse Cardiac Events In Patients With Acute Coronary Syndrome: A Six-Month Follow-Up Study, Nazlı Dilek Çolak, Turgut Karabağ, Onuralp Çalışkan, Songül Tezcan

Journal of the Saudi Heart Association

Objectives: Carbohydrate antigen 125 (CA125) is associated with different cardiovascular conditions. This study aimed to determine CA125 levels in patients with acute coronary syndrome (ACS) and the potential relationship between major adverse cardiac events (MACE) in the short-term following.

Methods: This prospective cohort study was conducted in a coronary care unit between May and November 2022. Plasma CA125 levels were measured only once upon hospital admission. Patients were followed for six months. All-cause mortality, recurrent acute coronary syndrome, requirement for revascularization, decompensated heart failure, cardiogenic pulmonary edema, atrial fibrillation, and stroke were recorded as MACE.

Results: A total of 127 …

Project Score Program Manual, M. Barnard, T. Johnson, A. Ford-Wade, M. Godfrey, C. Compretta, E. Dehon, S. Nasruddin, B. Wade, W. Sparkmon, M. Presley, A. Jones-White, W. Qualen

Faculty and Student Publications

Project SCORE (student-centered outcomes research experience) is an NIH-funded year-long informal public health education program and mentored research experience utilizing a youth participatory action research (YPAR) approach and near-peer mentors (NPMs) to increase science engagement, interest in college participation, and contribute to a diverse and community-engaged health sciences pipeline.

Building Health Career Pathways Early: A Community- Engaged Mini Medical School Model For Urban 4th Graders, Riley Kilzer, Liliana Bronner, Shirley F. Delair

Posters and Presentations: Family Medicine

No abstract provided.

Integrating Zotero With Your Writing: From Research To Publication For Urology Residency Program, Evelyn Wang

HSLIC Faculty and Staff Instructional Resources

This Urology Residency workshop introduces practical skills for integrating Zotero into research and writing workflows, from managing references to generating citations and bibliographies. Topics include installing and configuring Zotero and the Zotero Connector, collecting and organizing references with collections, tags, and PDFs, inserting citations and bibliographies in Microsoft Word and Google Docs, managing PDFs and annotations, and using advanced features such as sync, collaboration, custom citation styles, and ZoteroBib.

Improving Lunch Break To Enhance Nurse Happiness And Job Satisfaction, Evelyn O. Dumkwu

Doctor of Nursing Practice Projects

Nurses frequently forgo scheduled lunch breaks due to high workload demands, inadequate staffing, and organizational cultures. Consistently skipping breaks has been associated with reduced happiness and lower job satisfaction, which may ultimately compromise the quality of patient care. This quality improvement (QI) project examined whether the structured observance of scheduled lunch breaks enhances nurses’ subjective happiness and job satisfaction. Guided by Lewin’s Change Theory and the Donabedian Theoretical Framework and implemented using the Plan–Do–Study–Act (PDSA) framework, the project employed a quasi-experimental pre–post design during a six-week implementation period. Ten full-time nurses participated in the project. Investigator-designed Subjective Happiness and Job …

Social Support And Mental Toughness As Predictors Of Perceived Stress Among College Kinesiology Students, Jianmin Guan, Wan Xiang Yao, Xiaofen D. Hamilton, Gaofeng Lu

International Journal of Physical Activity and Health

Drawing on Lazarus and Folkman’s transactional theory of stress and coping, this study examined the associations of age, gender, three types of social support (family, friends, and significant others), and mental toughness with two dimensions of perceived stress (helplessness and coping inefficacy) among 666 college students majoring in Kinesiology. Hierarchical multiple regression analysis revealed that male college students, as well as those with higher levels of mental toughness and family support, reported significantly lower levels of helplessness and coping inefficacy in managing stress. Additionally, greater support from friends was significantly associated with lower levels of coping inefficacy, while younger students …

Genetic Modifiers Of Apoe-Ε4-Associated Cognitive Decline, Alex G Contreras, Carlos Cruchaga, Et Al.

2020-Current year OA Pubs

The APOE-ε4 allele is the strongest genetic risk factor for late-onset Alzheimer's disease. However, APOE-ε4 is not deterministic, highlighting the need to identify additional genetic and environmental factors. APOE-ε4 has been linked to accelerated cognitive decline, so we sought to investigate genetic factors that modify APOE-ε4-associated cognitive decline. We conduct cross-ancestry APOE-ε4-stratified and interaction GWAS using harmonized cognitive data from 32,778 participants, including 29,354 non-Hispanic White and 3,424 non-Hispanic Black individuals. Our primary outcome is late-life cognition, measured using harmonized composite scores for memory, executive function, and language, modeled as continuous traits reflecting both normative cognitive aging and disease-related decline. …

Quality Improvement Project: Effectiveness Of Staff Education On Fall Prevention, Theresa Oahre Okoyomo

Doctor of Nursing Practice Projects

Falls increase injury and mortality rates and decrease patients' quality of life. Hospital falls also incur enormous financial burdens on hospitals. In 2008, the Centers for Medicare & Medicaid Services (CMS) stopped reimbursing hospitals for fall-related injuries. The problem addressed in this quality improvement project was staff confidence in fall prevention and hospital fall rates. The purpose is to improve staff confidence and reduce fall rates by 95 % within six weeks. The 2025 CDC/STEADI Algorithm for Fall Risk Screening, Assessment, and Intervention was the program of choice because it aligns with the hospital's fall-prevention policy. This project design is …

Evaluating The Feasibility And Acceptability Of A Facilitator-Guided Wellness Initiative In A Residency Program, Alexander A. Missner, Jacob Gilbreth, Stacey Kaltman, Mayada Akil

Journal of Wellness

Introduction: Burnout in residency is a significant concern. There is an urgent need for feasible and transferable interventions that enhance resident well-being. Toward this end, we implemented a wellness intervention in a psychiatry residency program in 2019. Goals of the intervention included: 1) creating a safe space for sharing experiences in a supportive environment, 2) fostering group cohesion, and 3) addressing developmental challenges in training. We evaluated the feasibility and acceptability of the intervention.

Methods: This study utilized a mixed methods design. Following the 2022-2023 academic year, residents completed a survey examining their perceptions of how well the …

Gender Differences In College Students’ Physical Activity Self-Efficacy, Social Comparison Beliefs, And External Frames Of Reference, Ken Murfay, Sarah Pyszczynski, Sarah Collins

International Journal of Physical Activity and Health

This study examined whom college-aged individuals believe observing would more likely increase their physical activity self-efficacy (PASE) for a physical skill, whom they are more likely to compare their abilities to within a physical education (PE) class, how often they compare themselves to others in PE, and what students’ PASE scores were. Based on the mean scores for each question, students were more likely to compare themselves to a student than a teacher, friend than a non-friend, and similar or higher skilled peer than a lower skilled peer. To increase students’ confidence for a physical skill, students had higher levels …

Human Microglia In Brain Assembloids Display Region-Specific Diversity And Respond To Hyperexcitable Neurons Carrying Scn2a Mutation, Jiaxiang Wu, Xiaoling Chen, Jingliang Zhang, Kyle Wettschurack, Morgan Robinson, Weihao Li, Yuanrui Zhao, Ye-Eun Yoo, Brody A Deming, Yue Shu, Akila D Abeyaratna, Zhefu Que, Dongshu Du, Matthew Tegtmeyer, Chongli Yuan, William C Skarnes, Zhong-Yin Zhang, Jean-Christophe Rochet, Long-Jun Wu, Yang Yang

The Brown Foundation: Institute of Molecular Medicine

Microglia critically shape neuronal circuit development and function, yet their region-specific properties and roles in distinct circuits of the human brain remain poorly understood. In this study, we generated region-specific brain organoids (cortical, striatal, and midbrain), each integrated with human microglia, to fill this critical gap. Single-cell RNA sequencing uncovered six distinct microglial subtypes exhibiting unique regional signatures, including a subtype highly enriched for the GABAB receptor gene within striatal organoids. To investigate the contributions of microglia to neural circuitry, we created microglia-incorporated midbrain-striatal assembloids, modeling a core circuit node for many neuropsychiatric disorders, including autism. Using chemogenetics to activate …