Medicine and Health Sciences Commons^™

Professional Standards As Gateways, Not Barriers, In Occupational Therapy, Ryan Thomure

The Open Journal of Occupational Therapy

No abstract provided.

Understanding Graduate Occupational Therapy Educators’ Use Of Trauma-Informed Approaches In The Classroom, Sean M. Brim

The Open Journal of Occupational Therapy

Traumatic events can alter the brain structure and affect cognitive capacities and academic performance. Educators at all grade levels are responsible for supporting students through trauma-informed approaches. Previous trauma-informed research focused on K–12 education levels. The experience of graduate-level occupational therapy educators who have applied trauma-informed approaches to improve student learning have not been explored. This general qualitative study explored the experiences of occupational therapy educators who used trauma-informed approaches. The conceptual framework was the trauma-informed care model. Ten graduate-level occupational therapy educators with at least 1 year of teaching experience were recruited and participated in semi structured interviews. Data …

Perceived Educational Value Of Different Types Of Simulation In Occupational Therapy Education: A Mixed-Methods Study, Marie-Eve Bolduc, Barbara Shankland, Bernadette Nedelec

The Open Journal of Occupational Therapy

Simulation is an important instructional method in occupational therapy (OT) programs. However, it remains unclear if the various types of simulation hold the same educational value. The objective of this study was to determine the perceived educational value of three different types of simulations (role-play between students, live scenarios with simulated patients [SPs] and tele-simulation with SPs) and the factors that contributed to a higher educational value. This cross-sectional study used a sequential explanatory mixed-methods design. An online questionnaire was used, and two focus groups were conducted to develop a richer understanding of the answers obtained in the questionnaire. Forty-two …

Investigating The Efficacy Of An Online Multigenerational Awareness Course For Occupational Therapy Students – A Pilot Study, Susan Blair, Barbara M. Doucet, Jerilyn Callen, Kirsten Davin, Shelby Hutchinson

The Open Journal of Occupational Therapy

In today’s multigenerational society, particularly in health care settings, thriving can be challenging because of generational differences. Occupational therapy educational programs (OTEPs) prepare students for work environments where three to four different generations may be present. This study examines the effectiveness of an online multigenerational awareness (MGA) course. The secondary objectives include increasing students’ knowledge of enhanced multigenerational collaboration and communication strategies in preparation for future clinical practice. A pilot group of eight participants from various university departments reviewed the course content and completed preenrollment and postenrollment surveys, providing feedback regarding the applicability and perceived usefulness of the multigenerational awareness …

Intentional Resiliency Mentoring: A Mixed Methods Study Exploring A Pilot Mentorship Program For Occupational Therapy Students, Courtney J. Taylor, Ana K. Brussa, Kate G. Barlow

The Open Journal of Occupational Therapy

Because of high levels of reported stress, occupational therapy (OT) students’ ability to employ interpersonal skills and professionally manage conflicts is impeded. This mixed methods pilot study investigated the impact of an Intentional Relationship Model (IRM) informed mentorship program on OT students’ perceived stress and self-efficacy for interpersonal communication. Students (N = 21) completed pre and posttests of the Self Efficacy for Therapeutic Use of Self Scale (SETUS) and the Perceived Stress Scale (PSS-10). Six monthly mentor journals (N = 6) were collected to further explore the student experience. Quantitative findings demonstrated students’ (N = 21) ability to manage stress …

Occupational Therapy Students’ Perspectives On Using Virtual Reality Simulation In Preparation For Objective Structured Clinical Examinations: A Qualitative Study, Pegah Firouzeh, Craig R. St. Jean, Christine Ha, Mary Roduta Roberts

The Open Journal of Occupational Therapy

The use of virtual reality (VR) simulations in clinical education has been receiving increased research attention. The purpose of this study was to investigate first-year occupational therapy (OT) students’ experiences using a VR-simulated clinical interview as part of their preparation for a client history-taking Objective Structured Clinical Examination (OSCE). This qualitative study used focus groups to understand students’ (n = 20) perceptions of the effect of VR simulation on learning and exam readiness. We also explored students’ perceptions of the simulation’s authenticity, strengths, and weaknesses. Qualitative data analysis was conducted to identify codes and develop categories in association with the …

Ruth Coleman Denard (1906-2002): One Of The First Registered Black Occupational Therapists, Mirline Milien

The Open Journal of Occupational Therapy

The experiences and contributions of underrepresented groups in the occupational therapy profession are insufficiently explored and largely absent from occupational therapy education. For instance, the narratives of early Black occupational therapists and students are virtually nonexistent in the literature. As the profession moves toward greater inclusivity, it is essential to uncover these hidden stories and legacies to better understand the profession’s evolution and help shape its future. This article highlights Ruth Coleman Denard, one of the first registered Black occupational therapists in the United States, and provides an overview of her career trajectory. Historical research methods, including a review of …

An Occupation-Based Review Of Assessments Used In Pediatric Occupational Therapy Practice, Alysha Skuthan, Wendy B. Stav, Gracyn Mcvey, Jennifer Martinez, Marissa Hunter, Erin Diroll, Maureen Flanagan

The Open Journal of Occupational Therapy

Assessment tools are a critical component of the occupational therapy evaluation process. Despite the profession’s push toward occupation-centered practice, the classification of assessment tools as occupation-based versus skill-based does not exist. An occupation-based review of pediatric occupational therapy assessment tools was completed and scored according to the Occupation-Based Practice Assessment to classify assessment tools on a continuum of discrete or skill-based to occupation-based. Of the top 11 most frequently used tools, four were classified as occupation-based, three were classified as intermediate, and four were classified as discrete. In total, only 36% of commonly selected assessment tools are occupation-based. Pediatric occupational …

Maternal Role Transition: A Quality Of Life Frame Of Reference For Mothering After Nicu, Wendy Gaughan, Kathryn A. Lemonda

The Open Journal of Occupational Therapy

Occupational therapists are uniquely skilled to address the needs of a new mother and the infant during transition to motherhood, as acquisition of this new role may cause a role strain with other previously held roles. New mothers to infants requiring a neonatal intensive care unit (NICU) hospitalization experience additional stressors and considerations during this role transition. The Maternal Role Transition (MRT) Frame of Reference is a novel, community-based approach to facilitate attainment and integration of mothering occupations and address role strain during the perinatal period. MRT is rooted in the theoretical foundations of Maternal Role Attainment Theory, Self Efficacy …

Rehabilitation Habits And Roles: The Dual Impact Of Iadls And Psychosocial Interventions Poststroke, William Sit, Ryan Hulla, Veronica Smith, Joanna Ruiz, Steve Abraham

The Open Journal of Occupational Therapy

Background: This randomized study assessed an 8-week program for stroke survivors at an outpatient center in the United States. The program focuses on a holistic approach to recovery through habits and roles.

Method: Two researchers randomly divided 19 participants (N = 19) into two groups, with each group focusing on two distinct programs: the Occupational Therapy Instrumental Activities of Daily Living (IADLs) program and the Psychosocial Modules (PS) program for stroke survivors. Participants attended weekly group sessions and individual phone sessions, which reinforced learned strategies, provided personalized guidance, and ensured adherence to program goals. The Canadian Occupational Performance …

Enhancing Self-Esteem In Adolescents During Acute Inpatient Psychiatric Hospitalization: A Mixed Methods Study, Praveen Injeti, Julie D. Kugel, Dragana Krpalek, Liane Hewitt, Lida Gharibvand

The Open Journal of Occupational Therapy

Background: Low self-esteem (LSE) can negatively impact adolescent mental health, leading to suicidal ideation (SI). Social stressors, such as peer rejections, are considered a source of stress that can contribute to LSE, SI, and subsequent admission to psychiatric facilities. This study aimed to assess the effectiveness of an occupational therapy intervention to improve self-awareness and self-esteem during short-term hospitalization.

Method: The research used a mixed methods approach to evaluate the impact of a psychoeducational intervention by an occupational therapist on 16 adolescents. The Rosenberg Self-esteem Scale (RSES) was used before the intervention (pretest), immediately after the intervention (posttest …

A Polyvagal And Sensory Integration And Processing Approach To Supporting Development In Young Children With Autism, Catherine Cavaliere, Nadia Rust, Michelle Rogowski, Alyssa Calderone, Rebecca Marash, Amy Zdrodowski, John Damiao

The Open Journal of Occupational Therapy

Background: The polyvagal theory presents a framework for understanding the autonomic nervous system’s role in influencing behavior, engagement, and connection. Children with autism experience regulation and sensory integration and processing differences that impact their sense of felt safety and engagement in their environment. The Safe and Sound Protocol (SSP) is an auditory program designed to stimulate the vagal system and support nervous system regulation.

Methods: A retrospective multiple subject pretest-posttest design was used to examine the impact of the SSP on regulation, engagement, and skill acquisition in children with autism ages 2 to 3 enrolled in an early intervention center …

More Than Covering Ears: A Clinical Inventory For Characterizing Decreased Sound Tolerance (I-Dst) In Autism, Tana B. Carson, Angela Medina, Katherine L. Long, Yuxi Qiu, Monica Noriega

The Open Journal of Occupational Therapy

Background: Decreased sound tolerance (DST) is among the most common and distressing forms of sensory over-responsivity experienced by children with autism spectrum disorders. DST includes hyperacusis and misophonia, among others, and can limit participation in daily activities. Recently, a new screening tool, the Pediatric Hyperacusis and Misophonia Questionnaire (PMHQ-4) has been developed to allow health professionals to better identify and differentiate these conditions using a single assessment. Additional clinical tools are needed to further characterize DST symptoms to facilitate treatment planning after diagnosis.

Methods: In this mixed-methods study n = 15 parents of autistic children 6 to 17 …

State Of The Journal: Advancing And Promoting Frames Of Reference For Occupational Therapy Practice, Diane Powers Dirette

The Open Journal of Occupational Therapy

No abstract provided.

Whole Genome Sequence Analysis Of Pulmonary Function And Copd In 44,287 Multi-Ancestry Participants, Wonji Kim, Xiaowei Hu, Kangjin Kim, Sung Chun, Peter Orchard, Dandi Qiao, Ingo Ruczinski, Aabida Saferali, Francois Aguet, Lucinda Antonacci-Fulton, Pallavi P Balte, Traci M Bartz, Wardatul Jannat Anamika, Xiaobo Zhou, Junyi Duan, Jennifer A Brody, Brian E Cade, Martha L Daviglus, Harshavadran Doddapaneni, Shannon Dugan-Perez, Susan K Dutcher, Christian D Frazar, Stacey B Gabriel, Sina A Gharib, Namrata Gupta, Brian D Hobbs, Silva Kasela, Laura R Loehr, Ginger A Metcalf, Donna M Muzny, Elizabeth C Oelsner, Laura J Rasmussen-Torvik, Colleen M Sitlani, Joshua Smith, Tamar Sofer, Hanfei Xu, Bing Yu, David Zhang, John Ziniti, R Graham Barr, April P Carson, Myriam Fornage, Lifang Hou, Ravi Kalhan, Robert Kaplan, Tuuli Lappalainen, Stephanie J London, Alanna C Morrison, George T O'Connor, Bruce M Psaty, Laura M Raffield, Susan Redline, Stephen S Rich, Jerome I Rotter, Edwin K Silverman, Ani Manichaikul, Michael H Cho

Faculty, Staff and Student Publications

Background: Whole genome sequence (WGS) data in multi-ancestry samples supports discovery of low-frequency or population-specific genetic variants associated with chronic obstructive pulmonary disease (COPD) and lung function.

Results: We performed single variant, structural variant, and gene-based analysis of pulmonary function (FEV1, FVC and FEV1/FVC) and COPD case-control status in 44,287 multi-ancestry participants from the NHLBI Trans-Omics for Precision Medicine (TOPMed) Program. We validated findings using the UK Biobank and assessed implicated genes using lung single-cell RNA-seq (scRNA-seq) data sets. Applying a genome-wide significance threshold (P < 5 × 10-9), we replicated known loci and identified novel associations near LY86, MAGI1, GRK7, and LINC02668. Colocalization with gene expression quantitative trait loci (eQTL) from the Lung Tissue Research Consortium highlighted known candidate genes including ADAM19, THSD4, C4B, and PSMA4, which were not identified through other eQTL sources. Multi-ancestry analysis improved fine-mapping resolution (e.g., HTR4 and RIN3). Gene-based analysis identified and replicated HMCN1. In human lung scRNA-seq data sets, lung epithelial cells and immune cell types showed enriched expression, while fibroblasts showed higher expression for HMCN1. CRISPR targeting HMCN1 in IMR90 demonstrated reduced expression of collagen genes.

Conclusions: Large-scale multi-ancestry WGS analysis improves variant discovery and fine-mapping resolution for lung function and …

Beyond The Exam Room: Pediatricians Advocating Through State Aap, Heidi Sallee, Kelly Kreisler, Thuylinh Pham

Grand Rounds

1. Evaluate the current political climate and effects on child health at a National and State level.
2. Identify key child health policy issues for the states of Missouri and Kansas.
3. Use different forms of advocacy to share personal expertise.
4. Partner with state AAP Chapters to improve child health outcomes locally.

Precision Education In Health Sciences Education: A Call-To-Action For Developing An Interprofessional Strategy., Arvie Vitente, Jennifer Bosworth, Robert Sweet, Rebecca Cozzi, Christopher Galloway, Piotr Szczurek, Melanie Obispo-Young, Eron Bozec, Erica Brkovic, Tina Bobo, Jennifer Lubinski

Rehabilitation Sciences Department Faculty Articles

Introduction. Growing learner diversity, expanding biomedical knowledge, and rapid clinical digitization have exposed the limits of on-size-fits-all health sciences training. Competency-Based Education (CBE) has sharpened the focus on outcomes, yet many programs still move learners through fixed sequences, infrequent assessments, and retrospective remediation. Precision Education (PE) offers a complementary, data-enabled approach that uses learning analytics, artificial intelligence (AI), and continuous feedback loops. Embedded within CBE, PE converts competencies from static milestones into dynamic trajectories that can be measured, visualized, and adjusted in real time across the curriculum. This abstract highlights the need for an interprofessional strategy to design, fund, …

Teriparatide Use In Osteopenic Patients Undergoing Single-Level Lumbar Fusion Associated With Decreased 2-Year Revision Rates, Mitchell K. Ng, Ariel N. Rodriguez, Abigail Razi, Ahmed K. Emara, Brian T. Ford, Ameer Tabbaa, Divya Gouni, Jacquelyn J. Xu, Paul G. Mastrokostas, Jad Bou Monsef, Afshin E. Razi

Rothman Institute Papers

Context:  Low bone density is common among lumbar fusion patients and increases the risks of pseudarthrosis, hardware failure, and revision. Teriparatide improves bone mass and fusion in osteoporosis, but its benefit in osteopenia or normal bone remains uncertain.

Aims:  To evaluate the association between teriparatide exposure and (1) postoperative complications, (2) 90-day readmission, and (3) 2-year reoperation, stratified by bone health status (osteoporotic, osteopenic, and normal) in patients undergoing single-level lumbar fusion.

Setting and Design:  A retrospective cohort study using the PearlDiver database from 2010 to 2021 identified patients undergoing single-level lumbar fusion.

Subjects and Methods:  Patients on teriparatide were …

Knowledge, Attitude, And Practice Of Pharmacovigilance And Adverse Drug Reactions Reporting Among Healthcare Providers In The Middle East: A Systematic Review, Amina H. Saleem, Harith Kh. Al-Qazaz, Omer Q. Allela, Ramadan M. Elkalmi

BioMed Visions Journal

The main purpose of pharmacovigilance (PV) is to ensure medication safety through detecting, assessing, and preventing adverse drug reactions (ADRs). Healthcare providers (HCPs) are the main contributors in PV systems all over the world. However, their knowledge, attitudes, and practices (KAP) of PV and ADRs reporting vary among different medical professions and different countries.

Aim: To assess the knowledge, attitudes, and practices among the HCPs in the Middle East, toward PV and ADRs reporting.

Methods: A systematic review was performed. EBSCO, PubMed, and Semantic Scholar, FreeFullPDF and Google Scholar were searched for articles published from January 2014 to December 2024. …

Developing Pharmacy Education: Review Of Virtual Reality Technology In Improving Clinical Training And Learning Skill Development, Samer Imad Mohammed, Mohammed Khudhair Hasan, Mohammad Yawuz Jamal

BioMed Visions Journal

Incorporating modern technology into education is becoming imperative. Numerous pharmacy institutions are incorporating virtual reality (VR) technology training into their curricula to enhance educational experience. This review examines the current state, historical evolution, and application of VR programs in pharmacy education and training. The review also provides details about the main challenges and limitations associated with the use of this technology. The VR technology, including virtual laboratories and simulations, significantly improves clinical training and educational outcomes. The utilization of VR in clinical teaching encounters numerous barriers, including ethical concerns and technological constraints, as well as other restrictions in its execution …

The Impact Of Vdr Gene And Biochemical Indicators In Osteoporosis, Sabah Subhi Ismael Barani, Mohammed Abdullah Ajeel, Safwan Jasim Sultan

BioMed Visions Journal

Osteoporosis is a disorder caused by bone remodeling imbalance in which bone resorption outpaces bone production, resulting in weaker bone structure. Factors such as heredity and insufficient calcium and vitamin D consumption.

This study included an examination of 80 female patients of different ages (40–58 years) in Mosul. The participants were divided into two groups: 20 individuals served as the control group, while the rest 60 were osteoporotic women based on clinical cases and taking X ray DEXA. The blood samples are divided into two parts serum for biochemical tests and blood for genetic diversity in genes associated to Vitamin …

Postoperative Duodenal Stricture In A Neonate With Duodenal Atresia – Innovative Approach And Lessons Learned, Raniah I. Alnaser, Dr Mussalam L Alobaidy, Dr Fawaz A. Alassaf, Dr Mohammed N. Abed

BioMed Visions Journal

Background and aim: Duodenal atresia (DoA) is a congenital condition that is characterized by complete or incomplete duodenal obstruction. This condition needs urgent surgical intervention that carries risks of postoperative complications especially in low-birth-weight babies and with associated comorbidities. The success rate of the surgical procedure was reported to be higher than 90%. This case study details a neonate’s clinical course reporting the challenge in the management of delayed stricture and malnutrition after the primary surgery.

Case presentation and results: A female neonate was born at 38 weeks of gestation with 2.6 kg weight. During the immediate postnatal period, she …

Knowledge And Diagnostic Skills Of Dental Residents Towards Endodontically Involved Teeth In Specialized Dental Center In Mosul City / Iraq, Huda Kh. Abdulkader, Dina Waadallah Azeez

BioMed Visions Journal

Objectives: to assess the knowledge regarding the diagnosis of endodontically affected teeth and the decision-making abilities regarding specific clinical situations among the dental residents in dental specialist center/ left bank/ Mosul / Iraq.

Materials & method: A self–administered questionnaire consisting of 20–questions was distributed to the dental residents at the Dental Specialist Center on the Left Bank in Mosul city, Iraq, in order to assess their diagnostic skills and decision-making abilities in relation to endodontically involved teeth. Additionally, the impacts of taking an endodontic course were investigated. SPSS version 21 was used for data analysis.

Results: Forty-one dental residents participated …

Helicobacter Pylori Infection’S Impact On Thyroid Dysfunction And Serum Interleukin-6 Levels, Mohamed M. Sehree, Ashraf R. Salem Al-Safar

BioMed Visions Journal

Helicobacter pylori is a Gram-negative bacterium associated with peptic ulcer disease and gastric cancer. The study aimed to evaluate its prognosis and its relationship to sex, age, thyroid function, IL-6, and blood groups. 120 blood samples were collected from patients with gastrointestinal disorders at Al-Salam Hospital in Mosul between May and October 2024. The samples underwent clinical examination, including anti-H. pylori antibodies (chromatography and IgA ELISA to confirm active infection), thyroid hormones (Minvidus), interleukin-6 (ELISA), and blood typing using serological techniques.

The study results revealed that the rapid test revealed 40 positive cases out of 120, while the ELISA (IgA) …

Sirtuins And Cartilage Preservation: From Cellular Function To Therapeutic Innovation, Muthanna K. Zaki, Khalil A. Hadid, Mohammed N. Abed, Fawaz A. Alassaf

BioMed Visions Journal

The bones are cushioned by a thin layer of hyaline cartilage that permits physical activity to be performed without experiencing any discomfort. However, these tissues are susceptible to several diseases involving osteoarthritis and rheumatoid arthritis resulting in cartilage degradation. Sirtuins (SRTs) and SRT-activating molecules have been shown in previous studies to affect cartilage and chondrocytes positively. This review aims to explore the potential protective effects of SRTs on cartilage and chondrocytes. It also highlighting various SRT-activating substances and their potential therapeutic applications in managing cartilage-related diseases. Google Scholar, Cochrane Library, and PubMed were used to search about related studies. The …

Anoikis Resistance And Metastasis Of Ovarian Cancer Can Be Overcome By Cdk8/19 Mediator Kinase Inhibition, Mehri Monavarian, Resha Rajkarnikar, Emily Faith Page, Asha Kumari, Liz Quintero Macias, Felipe Massicano, Nam. Y. Lee, Sarthak Sahoo, Nadine Hempel, Mohit Kumar Jolly, Lara Ianov, Elizabeth Worthey, Abhyudai Singh, Igor Roninson Ph.D., Eugenia Broude Ph.D., Mengqian Chen Ph.D., Karthikeyan Mythreye

Faculty Publications

Anoikis resistance, or evasion of cell death triggered by matrix detachment, is a hallmark of cancer cell survival and metastasis. We showed that repeated exposure to suspension stress followed by recovery under attached conditions leads to development of anoikis resistance. The acquisition of anoikis resistance was associated with enhanced invasion, chemoresistance, and immune evasion in vitro and distant metastasis in vivo. This acquired anoikis resistance was not genetic, persisting for a finite duration without detachment stress, but was sensitive to CDK8/19 mediator kinase inhibition that could also reverse anoikis resistance. Transcriptomic analysis revealed that CDK8/19 kinase inhibition induces bidirectional transcriptional …

“It Seems Like A Never-Ending Job”: Voices Of Female Caregivers Of Older Adults In The Rural Communities, Lalani Nasreen, Bhagyashree Katare, Evans Appiah Osei, Siqi Yang, Sampada Wagle, Julian L. Gallegos, Abidemi Mary Ajuwon

School of Nursing Faculty Publications

Background: Rural female caregivers of older adults face significant caregiving challenges that puts them at high risk for poor self-care and wellbeing. Limited studies have examined the self-care needs of caregivers from a gender equity and social perspective.

Objective: Our study aims to explore the self-care needs and preferences of rural female caregivers and underlying key processes contributing toward their health and well-being. Methods: A qualitative descriptive design was used for the study. A purposive sample of (n = 20) rural female caregivers was obtained. In-depth individual interviews were conducted for data collection. Each interview was about 45- 60 min …

Socioecological Stressors During Covid-19 Among Urban Refugee Youth In Kampala, Uganda: A Qualitative Descriptive Analysis, Sikky Shiqi Chen, Carmen H. Logie, Frannie Mackenzie, Moses Okumu, Robert Hakiza, Brenda Katisi, Daniel Kibuuka Musoke, Aidah Nakitende, Isha Berry, Bay Bahri, Peter Kyambadde

Journal of Social, Behavioral, and Health Sciences

Limited studies have examined pandemic-related stressors among urban refugee youth in low and middle-income humanitarian countries (LMICs), such as Uganda. We explored the lived experience of COVID-19-related stressors among urban refugee youth in Kampala, Uganda. We analyzed qualitative data (text messages, WhatsApp chats, photos) collected in an interactive mobile health intervention with 346 refugee youth aged 16–24 in Kampala. Findings revealed stressors across socioecological levels: individual (e.g., financial-related distress), social (e.g., limited social interactions), community (e.g., food insecurity), and policy (e.g., unprecedented lockdowns). Findings can inform social work organization pandemic preparedness strategies for economic empowerment and social cohesion with youth.

Development Of The Self-Administered Health Complexity Screening Instrument, Sheila Specker, Rachel Andrew, Emily Drexler, Emily Koithan, Steven Thurber, Steven Frankel

School of Medicine Faculty Publications

PURPOSE: The purpose of the study was to develop a self-administered screening tool to assist case managers, primary care physicians, and other clinicians in quickly and accurately identifying patients with complex health needs who require a definitive and detailed case management evaluation. In addition to medical and psychiatric items, this tool incorporates multiple social determinants of health (SDOH), known to add to clinical complexity. PRIMARY PRACTICE SETTING: This instrument is most appropriate for identifying patients with high complexity in the primary care sector. Due to the abundance of such patients with high biopsychosocial complexity and costs of treating these patients, …

Rab5c Increases Endothelial Release Of Vwf By Regulating Vesicle Trafficking, Paula Reventun, Pablo Toledano-Sanz, Maria Delgado-Marin, Maria Viskadourou, D Brian Foster, Paul S De Vries, Maria Sabater-Lleal, Nunzio Alcharani, Claudia Gonzalez-Cucharero, William O Osburn, Alanna C Morrison, Alisa S Wolberg, Nicholas L Smith, Marios Arvanitis, Charles J Lowenstein

Faculty, Staff and Student Publications

Background: Abnormal levels of VWF (von Willebrand Factor) are a risk factor for venous thromboembolism (VTE) and bleeding. Genome-wide association studies for VWF have identified novel candidate genes that may regulate VWF levels in humans, including RAB5C (RAS-associated protein RAB5C). We hypothesized that RAB5C regulates VWF release from endothelial cells.

Methods: We studied the effect of RAB5C on vesicle trafficking in human endothelial cells. We performed CRISPR (clustered regularly interspaced short palindromic repeats) interference targeting 2 genetic variants linked to altered VWF levels and evaluated RAB5C expression by reverse transcription-quantitative polymerase chain reaction. We silenced RAB5C or overexpressed RAB5C wild-type, …