Medicine and Health Sciences Commons^™

Implementing Standardized Patients In Simulation In Undergraduate Nursing Education, Taylor A. Murray

SACAD: Scholarly Activities

Abstract

The integration of standardized patients (SPs) in simulation-based learning has emerged as a transformative approach in undergraduate nursing education. By providing nursing students with the opportunity to engage with trained actors simulating real-life patient scenarios, SPs offer a safe and controlled environment to develop essential clinical skills. This research explores the impact of SPs on promoting student confidence and enhancing their practical skills in nursing practice. Implementing SPs into nursing education not only supports students in refining their assessment and communication abilities but also prepares them for the complexities of patient care in real-world settings. Ultimately, this study aims …

Development Of A Synthetic Biomarker System For Early Detection Of Myocardial Infarction, Kennedy Alexis Bietz

Honors Thesis

Every year, thousands of Americans suffer from the event of a heart attack, i.e., myocardial infarction (MI). Currently, the early detection of MI remains a challenge. We intend to solve the problem by constructing a synthetic biomarker system that would be able to recognize early warning signs of MI, such as cellular inflammation and oxidative stress. We have proposed two different pathology specific genetic sensors that act as artificial promoters to transcribe a peptide that can be secreted into the blood stream and eventually work its way into the urine and act as a reporter that can be detected through …

A Life Course Approach To Understanding Preserved Cognition In Late Life, Cassidy E. Doyle

USF Tampa Graduate Theses and Dissertations

Some level of cognitive decline is generally expected with advanced age. However, a subgroup of individuals – commonly labeled as ‘SuperAgers’ – maintain youthful memory abilities into their 80s and beyond. Relatively little is known about factors across the life course related to this phenotype. This dissertation used population-based data from the Health and Retirement Study (HRS) to investigate lifestyle factors related to the most youthful cognitive trajectory in the United States and assess how their cognition fares over time. Three research questions were addressed: (1) Can samples of youthful cognitive agers be identified longitudinally in a population-based dataset? (2) …

Breast Cancer Diagnoses Are Increasing Among Racial And Ethnic Minority Women In The U.S., Esther Oshaji

Population Health Research Brief Series

Breast cancer is the second most common cancer and a leading cause of cancer deaths among women in the United States. This data slice uses data from the Surveillance, Epidemiology and End Results Program (SEER) Incidence Database to examine trends in breast cancer incidence rates among women by race and ethnicity in the U.S. from 2000 to 2021. The author finds that, while incidence rates remain highest among non-Hispanic White women, the largest increases in diagnoses have been among American Indian/Alaska Native Asian/Pacific Islander women.

The Nih Is A Sound Investment For The Us Taxpayer, Nicholas W. Gilpin

School of Medicine Faculty Publications

Research funded by the National Institutes of Health is essential for improving the health of Americans and developing new drugs and treatments for a wide range of diseases.

Standardizing Warming Measures For Surgical Patients, Brachelle Williams, Bsn, Rn

Doctor of Nursing Practice Final Project Abstract

Purpose

The quality improvement project aimed to address the risk of perioperative hypothermia in surgical patients by standardizing warming measures at a Houston, Texas boutique hospitals surgical department. The focus was to achieve normothermia (≥ 96.8°F) within 10 minutes of PACU arrival and increase patient satisfaction by at least 50% through the implementation of a forced air warming system.

Background

Maintaining normothermia is crucial for surgical patients, as hypothermia can lead to delayed recovery and increased postoperative discomfort. The Houston’s boutique hospital previously solely relied on warm blankets, resulting in inconsistent temperature management.

Methodology

The Plan-Do-Study-Act (PDSA) model guided the …

Rising Incidence Of Acute Hepatitis A Among Adults And Clinical Characteristics In A Tertiary Care Center Of Pakistan, Yumna Shahid, Amna Subhan Butt, Iqra Jamali, Faisal Wasim Ismail

Section of Gastroenterology

Background: For decades, hepatitis A virus (HAV) has been a leading cause of acute hepatitis among children and was less prevalent among adults. However, recently a paradigm shift has been observed in the epidemiology of HAV, as evident by cases of acute hepatitis due to HAV among adults.
Aim: To estimate frequency of HAV in acute viral hepatitis and compare characteristics in HAV and hepatitis E virus (HEV) infection.
Methods: This was a trend analysis conducted at Aga Khan University Hospital Karachi (Sindh, Pakistan) from February 2024 to May 2024. Individuals aged 18 years and older diagnosed with acute viral …

A Green Validated Hplc-Uv Method For Determining And Quantifying Pholcodine, Paracetamol, And Pseudoephedrine In Laboratory-Prepared Mixtures And Their Fdc Capsule, Ekram Hany Mohamed, Mennatallah Ahmed Hesham, Ehab Elkady Prof, Sally Mahmoud Tarek

Pharmacy

A validated technique has been developed for the sequential determination of pholcodine, paracetamol, and pseudoephedrine. This method allows for their separation either in different ratios within a ternary mixture or in their fixed-dose combination (FDC) using HPLC. The chromatographic conditions involved the utilization of an X-select C18 column (5µ, 4.6 × 150 mm) and a mobile phase consisting of 0.05 M KH2PO4: Methanol (75:25, v/v), with orthophosphoric acid added to adjust the pH to 2.6. The separation was conducted at a 1.2 ml/min flow rate, while a UV detector was set at a wavelength of 206 nm to monitor the …

Aerosolized Transmissible Diseases Guidance Document, J. David Miller, John P. (Jack) Springston Jr., Cheryl L. Marcham, J. David Krause, Ling-Ling Hung, Wane A. Baker

Publications

Prior to COVID-19, pandemic influenza viruses have emerged four times in the previous one hundred years. This includes “Spanish” influenza (H1N1) in 1918, “Asian” influenza (H2N2) in 1957, “Hong Kong” influenza (H3N2) in 1968, and H1N1 “swine” influenza in 2009 (Saunders-Hastings & Krewski, 2016; Taubenberger, 2005). In early May 2024, the first reported human case of avian flu (H5N1) that had also infected cows in the US appeared in the literature (Uyeki et al., 2024). In late August 2024, OSHA released a hazard alert providing information for dairy workers on avian influenza in dairy cattle, together with personal protective equipment …

The Incidence Between Carpal Tunnel Syndrome (Cts) And De Quervain’S Tenosynovitis (Dqt) Using Electrophysiological And Ultrasonography Study., Shaimaa M. Abd El Aziz, Lamis Safwat

Journal of Medicine in Scientific Research

One of the most common entrapment neuropathies affecting the upper limbs is carpal tunnel syndrome (CTS). de Quervain tenosynovitis (DQT) is a condition that affects the wrist's first dorsal compartment and is characterized by tendon entrapment and thickening of sheaths around the abductor pollicis longus and extensor pollicis brevis. The association of idiopathic CTS and idiopathic DQT has been studied in a few researches. In this study, we would like to summarize the most recent knowledge about the occurrence of CTS and DQT on the same hand using electrophysiological and ultrasonography studies.

Does Coronary Artery Bypass Grafting Surgery Affect The Right Ventricular Function Postoperatively?, Ahmed A Faragalla, Ahmed M Elshiemy, Sherif A Elhindawy, Ahmed A Abdelhamid

Journal of Medicine in Scientific Research

Background A well-established benefit of coronary artery surgery (CABG) is to improve the left ventricular (LV) performance meanwhile, its impact on the right ventricular (RV) chamber is obscure and an issue of continuous investigations. Aim This study explores the influence of CABG on the RV performance. Methods This study employed a prospective longitudinal, observational design to evaluate function in 30 patients undergoing isolated CABG. Echocardiography with a transthoracic approach was accomplished preoperatively, one week and one-month post-surgery. The myocardial performance index (Tei index), tricuspid annular plane systolic excursion (TAPSE), and peak systolic (Sm) and early diastolic (Em) myocardial velocities at …

Chemoid-Guided Therapy Improves Objective Response Rate In Recurrent Platinum-Resistant Ovarian Cancer Randomized Clinical Trial, Thomas J. Herzog, Thomas C. Krivak, Stephen Bush Ii, John P. Diaz, Scott Lentz, Navya Nair, Nadim Bou Zgheib, Camille Gunderson-Jackson, Abhijit Barve, Krista L. Denning, Seth T. Lirette, Candace M. Howard, Jagan Valluri, Pier Paolo Claudio

School of Medicine Faculty Publications

Patients with recurrent platinum-resistant ovarian cancer (PROC) have poor clinical outcomes, owing mainly to the presence of therapy-resistant cancer stem cells (CSCs). The NCT03949283 randomized clinical trial enrolled patients with recurrent PROC to receive ChemoID-guided chemotherapy or the best physician-choice regimen selected from the same list of thirteen mono or combination chemotherapies. The primary outcome was objective response rate (ORR) assessed on CT scans using the RECIST 1.1 criteria at 6 months follow-up. Subjects treated with the ChemoID assay had an ORR of 55% (CI(95) 39% - 73%), compared to 5% (CI(95) 0% - 11%) for those treated with physician’s …

Unlocking Precision Using K-Means++- Improved Genetic Algorithm-Radial Basis Function Neural Network: Data-Driven Evolution Of Smart Gloves For Gesture Recognition, Liang Xiao Ding, Kuan Way Chee, Hong Lü, Anand Paul, Jeonghong Kim, Jang Myung Lee

School of Public Health Faculty Publications

Human-computer interaction technologies have been used since the 1970s but have only gained growing popularity in recent years with new design paradigms. Ongoing research and development in gesture recognition systems with broad application prospects have focused on improving accuracy and real-time performance as well as the robustness of specific machine learning algorithms against environmental conditions. This paper addresses the accuracy enhancement of a novel Fifth Dimension Technologies data-glove-based gesture recognition system using a genetic-algorithm (GA)-trained k-means++-improved radial basis function (RBF) or GK-RBF neural network. First, we analyzed and modeled the sensor distribution in the data glove and proposed joint constraints …

Paving The Way To Practice For International Nurses, Jaime Kupillas Msn, Rn, Kaitlyn Goodson Msn, Rn

Department of Education

No abstract provided.

Multicilia Dynamically Transduce Sonic Hedgehog Signaling To Regulate Choroid Plexus Functions, Suifang Mao, Michael J Holtzman, Et Al.

2020-Current year OA Pubs

The choroid plexus is a major site for cerebrospinal fluid (CSF) production, characterized by a multiciliated epithelial monolayer that regulates CSF production. We demonstrate that defective choroid plexus ciliogenesis or intraflagellar transport yields neonatal hydrocephalus, at least in part due to increased water channel Aqp1 and ion transporter Atp1a2 expression. We demonstrate choroid plexus multicilia as sensory cilia, transducing both canonical and non-canonical Sonic Hedgehog (Shh) signaling. Interestingly, it is the non-canonical Shh signaling that represses Aqp1 and Atp1a2 expression by the Smoothened (Smo)/Gαi/cyclic AMP (cAMP) pathway. Choroid plexus multicilia exhibit unique ciliary ultrastructure, carrying features of both primary and …

A Network Correspondence Toolbox For Quantitative Evaluation Of Novel Neuroimaging Results, Ru Kong, Evan M Gordon, Et Al.

2020-Current year OA Pubs

The brain can be decomposed into large-scale functional networks, but the specific spatial topographies of these networks and the names used to describe them vary across studies. Such discordance has hampered interpretation and convergence of research findings across the field. We have developed the Network Correspondence Toolbox (NCT) to permit researchers to examine and report spatial correspondence between their novel neuroimaging results and multiple widely used functional brain atlases. We provide several exemplar demonstrations to illustrate how researchers can use the NCT to report their own findings. The NCT provides a convenient means for computing Dice coefficients with spin test …

Prevalence Of Psychiatric And Sleep Disorders And Their Impact On Quality Of Life In Children With Hypermobile Ehlers-Danlos Syndrome: An Observational Study., Amanda K. Hertel, Jordan T. Jones, Ashley Lytch, Emily Cramer, Ariana Schroeder, William R. Black

Manuscripts, Articles, Book Chapters and Other Papers

Hypermobile Ehlers-Danlos Syndrome (hEDS) is the most common connective tissue disorder. However, few studies exist on psychiatric and sleep disorders in pediatric patients with hEDS. This study aims to describe psychiatric and sleep disorders and evaluate their impact on health-related quality of life (HRQoL) in pediatric patients with hEDS. As part of a longitudinal study, a convenience sample of 123 pediatric patients with hEDS, were recruited at a hEDS multidisciplinary clinic in sequential order over a seven-month period. Patient-reported outcomes were completed (Patient Reported Outcomes Measurement Information System Pediatric Profile Version 2 [PROMIS], Generalized Anxiety Disorder-7 [GAD-7], Adolescent Sleep Wake …

Role Of Eye-Tracking Technology And Software Algorithms In Enhancing Adhd Detection And Diagnosis: A Systematic Literature Review, Lauren E. Perkins

Honors College Theses

This systematic literature review explores the role of eye-tracking technology and software algorithms in enhancing the detection and diagnosis of ADHD. ADHD, a neurodevelopmental disorder affecting both children and adults, is traditionally diagnosed through behavioral assessments, which may lack objectivity. Recent studies suggest that eye-tracking, specifically focusing on saccades, fixations, and blink rates, offers the potential for more accurate and objective measures of ADHD. The review examines clinical trials, observational studies, and machine learning research to assess the correlation between ADHD and eye movement patterns. Results indicate that individuals with ADHD exhibit distinct eye movement patterns, which can be quantified …

Perceived Relevance Of Planetary Health For Medical Students In Italy: Results From A Mixed-Methods Analysis, L. Nachira, P. Arcaro, F. Pattavina, E. Campo, C. Castagna, R. Frasso, C. Cadeddu, S. Bruno

College of Population Health Faculty Papers

Objectives: To address the growing hazards to human health caused by the anthropogenic environmental catastrophe, academic curricula at all levels and disciplines, particularly medical education, should incorporate Planetary Health Education. This study aims to examine medical students' expectations and feedback on a Planetary Health elective in an Italian University.

Study design: Cross-sectional study, employing a convergent parallel mixed-methods design. Methods: Attendees were invited to take part in a mixed-methods written survey, integrating both quantitative and qualitative methodologies. A statistical and a thematic analysis were conducted to assess responses to questions of a quantitative and qualitative nature, respectively.

Results: Interest in …

Obesity Awareness Among Pregnant Hispanic Women In Urban New Jersey, Cloris Bustamante

Walden Dissertations and Doctoral Studies

The rising prevalence of obesity among pregnant women presents significant health risks for mothers and children, beginning as early as intrauterine life throughout adulthood. The purpose of this quantitative cross-sectional study was to investigate whether demographic factors influenced obesity awareness as a personal issue in pregnant Hispanic women. The precaution adoption process model guided the study, a theoretical framework focusing on stages of health behavior change, including awareness, decision making, and action. A purposive sample of 134 pregnant Hispanic women aged 18 years or older receiving prenatal care in urban New Jersey was surveyed using the English (15) and Spanish …

Effects Of Intensive Blood Pressure Treatment On Orthostatic Hypertension: Individual Level Meta-Analysis, Stephen P Juraschek, Jiun-Ruey Hu, Jennifer L Cluett, Carol Mita, Lewis A Lipsitz, Lawrence J Appel, Nigel S Beckett, Barry R Davis, Rury R Holman, Edgar R Miller, Kenneth J Mukamal, Ruth Peters, Jan A Staessen, Addison A Taylor, Jackson T Wright, William C Cushman

Faculty, Staff and Students Publications

Objective: To determine the effects of intensive blood pressure treatment on orthostatic hypertension.

Design: Systematic review and individual participant data meta-analysis.

Data sources: MEDLINE, Embase, and Cochrane CENTRAL databases through 13 November 2023.

Inclusion criteria: Population: ≥500 adults, age ≥18 years with hypertension or elevated blood pressure; intervention: randomized trials of more intensive antihypertensive drug treatment (lower blood pressure goal or active agent) with duration ≥6 months; control: less intensive antihypertensive drug treatment (higher blood pressure goal or placebo); outcome: measured standing blood pressure.

Main outcomes: Orthostatic hypertension, defined as an increase in systolic blood pressure ≥20 mm Hg or …

Molecular Basis For Human Respiratory Syncytial Virus Transcriptional Regulator Ns1 Interactions With Med25, Parismita Kalita, Oam Khatavkar, Grace Uwase, Yulia Korshunova, Yuying Hu, Nicole D Wagner, Jian Xu, Jiehong Pan, Jay C Nix, Michael L Gross, Steven L Brody, Dominika Borek, Gaya K Amarasinghe, Jacqueline E Payton, Daisy W Leung

2020-Current year OA Pubs

The Mediator complex facilitates interactions between transcription factors and RNA polymerase II, a process that is required for host gene transcription, including in response to viral infections. Among the many subunits in the Mediator complex, the MED25 subunit has been shown to be a target for viral activators during infection. Here we provide the molecular basis for the interaction between human respiratory syncytial virus (hRSV) nonstructural 1 protein (NS1) and the activator interaction domain (ACID) of MED25. The X-ray crystal structure of the complex revealed that NS1 straddles and binds two faces of MED25 ACID. This interaction is distinct from …

American Society Of Hematology Living Guidelines On Use Of Anticoagulation For Thromboprophylaxis For Patients With Covid-19: Executive Summary, Deborah M Siegal, Kristen M Sanfilippo, Yazan Al Jabiri, Et Al.

2020-Current year OA Pubs

BACKGROUND: COVID-19-related critical and acute illness is associated with an increased risk of venous thromboembolism (VTE). These evidence-based recommendations of the American Society of Hematology (ASH) are intended to support patients, clinicians, and other health care professionals in decisions about using anticoagulation for thromboprophylaxis for patients with COVID-19-related critical illness; patients with COVID-19-related acute illness; and those being discharged from the hospital, who do not have suspected or confirmed VTE.

METHODS: ASH formed a multidisciplinary panel, including patient representatives. The Michael G. DeGroote Cochrane Canada and MacGRADE Centres at McMaster University supported guideline development, including performing systematic reviews (up to …

Trends In Resident Cataract Surgery Complication Rates Under A Single Experienced Supervising Attending, Simran Ohri, Jason A. Zehden, Dipen Kumar, Roma Pegany, Zhidong Zhang, Kenneth Cohen, Alice Y. Zhang

Teaching and Learning Faculty Publications

Purpose: To evaluate resident surgical complication rates under the supervision of an experienced attending surgeon over five years.

Setting: A single academic medical center at University of North Carolina at Chapel Hill, Chapel Hill, NC.

Design: Retrospective chart review.

Methods: Retrospective review of the first 30e50 cases performed by five classes of residents supervised by a single experienced attending surgeon with over twenty years of experience as a surgical educator. Data was collected from Accreditation Council for Graduate Medical Education (ACGME) surgical logs and operative notes. Preoperative risk factors, operative technique, and intraoperative complication rates were analyzed.

Results: A total …

Cell-Matrix Feedback Controls Stretch-Induced Cellular Memory And Fibroblast Activation, Yuan Hong, Xiangjun Peng, Haomin Yu, Delaram Shakiba, Yuxuan Huang, Chengqing Qu, Aliza Mujahid, Yin-Yuan Huang, Jacob A Sandler, Kenneth M Pryse, Justin M Sacks, Elliot L Elson, Guy M Genin, Et Al.

2020-Current year OA Pubs

Mechanical stretch can activate long-lived changes in fibroblasts, increasing their contractility and initiating phenotypic transformations. This activation, critical to wound healing and procedures such as skin grafting, increases with mechanical stimulus for cells cultured in two-dimensional but is highly variable in cells in three-dimensional (3D) tissue. Here, we show that static mechanical stretch of cells in 3D tissues can either increase or decrease fibroblast activation depending upon recursive cell-extracellular matrix (ECM) feedback and demonstrate control of this activation through integrated in vitro and mathematical models. ECM viscoelasticity, signaling dynamics, and cell mechanics combine to yield a predictable, but nonmonotonic, relationship …

Carotid Peak Flow Velocity Variation As A Surrogate Of Aortic Peak Flow Velocity Variation In A Pediatric Population, Federico Cristiani, Juan Pablo Bouchacourt, Juan Riva, Pablo Motta

Faculty, Staff and Students Publications

Background: Carotid peak velocity variation (ΔVpeakCar) is an alternative to aortic peak velocity variation (ΔVpeakAo) and has been used in the pediatric population. Children's physiology and anatomy are heterogeneous throughout their growth. For this reason, the predictive value of ΔVpeakCar as a surrogate of ΔVpeakAo can vary at different ages. We hypothesize that the ability of ΔVpeakCar as a surrogate of ΔVpeakAo changes throughout childhood.

Aim: Analyze the concordance and the tracking ability of ΔVpeakCar and the ΔVpeakAo at different stages of development.

Methods: Patients from 0 to 12 years were included. Three groups were defined: under 12 months (G1), …

Haploinsufficiency Of Itsn1 Is Associated With A Substantial Increased Risk Of Parkinson’S Disease, Thomas P Spargo, Chloe F Sands, Isabella R Juan, Jonathan Mitchell, Vida Ravanmehr, Jessica C Butts, Ruth B De-Paula, Youngdoo Kim, Fengyuan Hu, Quanli Wang, Dimitrios Vitsios, Manik Garg, Lawrence Middleton, Michal Tyrlik, Mirko Messa, Guillermo Del Angel, Daniel G Calame, Hiba Saade, Laurie Robak, Ben Hollis, Vishnu A Cuddapah, Huda Y Zoghbi, Joshua M Shulman, Slavé Petrovski, Ismael Al-Ramahi, Ioanna Tachmazidou, Ryan S Dhindsa

Faculty, Staff and Students Publications

Despite its significant heritability, the genetic basis of Parkinson's disease (PD) remains incompletely understood. Here, in analyzing whole-genome sequence data from 3,809 PD cases and 247,101 controls in the UK Biobank, we discover that protein-truncating variants in ITSN1 confer a substantially increased risk of PD (p = 6.1 × 10

The Feasibility And Safety Of Robot-Assisted Vaginal Natural Orifice Transluminal Endoscopic Surgery (Ra-Vnotes) For Gynecologic Disease: 298-Case Series, Qiannan Yang, Daniel Y Lovell, Yingchun Ma, Chunhua Zhang, Xiaoming Guan

Faculty, Staff and Students Publications

Objectives: To explore the feasibility and surgical outcomes of robot-assisted vaginal natural orifice transluminal endoscopic surgery (RA-vNOTES) for women suffering from gynecologic disease.

Methods: We performed an observational study reporting and analyzing the perioperative outcomes of 298 patients with gynecologic disease who underwent RA-vNOTES in a single institution from June 2019 to August 2024.

Results: A total of 298 patients with a median age of 41 years and median body mass index of 29 kg/m2 underwent RA-vNOTES. The primary indications for surgery were endometriosis (43.62%), chronic pelvic pain (11.07%), abnormal uterine bleeding (20.81%), and uterine leiomyomata (14.77%). A total of …

Spatial And Seasonal Dynamics Of Mosquito Species In Harris County, Texas, Highlight Risk Of Arbovirus Introduction And Transmission, Morgan Jibowu, Maximea Vigilant, Andre Luis Costa-Da-Silva, Alisa Nelson, Heather T Essigmann, Eric L Brown, Sarah M Gunter

Faculty, Staff and Student Publications

Mosquito-borne diseases pose a significant public health threat, particularly in densely populated urban areas. Climate change, urbanization, and global connectivity have fueled the expansion of mosquitoes and their associated pathogens, increasing the disease burden. Harris County, Texas, is among the most vulnerable regions in the United States for mosquito-borne disease outbreaks, underscoring the critical need for localized insights into mosquito population dynamics to inform vector surveillance and control strategies. This study provides a comprehensive analysis of the population dynamics of mosquito species in Harris County by exploring their species composition, spatial distribution, and seasonal patterns. Our findings reveal the extensive …

Rapid Laser Ablation-Based Fabrication Of High-Density Polymer Microwell Arrays For High-Throughput Cellular Studies, Desh Deepak Dixit, Kavya L Singampalli, Amit S Niyogi, Amanda Montoya, Alexandre Reuben, Peter B Lillehoj

Faculty, Staff and Student Publications

Polymer-based microwell platforms have garnered much interest due to their usefulness in culturing and analyzing small quantities of biological cells and spheroids. Existing methods for fabricating polymer microwell arrays involve complex fabrication processes and/or are limited in their ability to create dense arrays of very small (< 50 μm in diameter) microwells. Here, we present a simple and rapid technique for fabricating high-density arrays of microwells ranging from 20 to 160 μm in diameter on a variety of polymer substrates. In this approach, a polymer surface is ablated using a CO2 laser that is rastered over a stainless steel mesh, which serves as a shadow mask. A theoretical laser-polymer interaction model was developed for predicting the microwell volume based on the substrate properties and laser settings. Microwell volumes predicted by the model were within 5.4% of fabricated microwell volumes determined experimentally. Cellulose acetate microwell arrays fabricated using this technique were used to culture Lewis lung carcinoma cells expressing ovalbumin (LLC-OVA), which were maintained for up to 72 h with a negligible (< 5%) loss in viability. As a second proof of principle demonstration, LLC-OVA cells grown in microwell arrays were co-cultured with OT-I T cells and measurements of interferon gamma (IFN-γ), a marker for T cell activation, were performed which revealed a positive correlation between LLC-OVA cell-T cell interaction time and T cell activation. These two in vitro demonstrations showcase the capability of this technique in generating polymer microwell arrays for high-throughput cellular studies, including cell growth dynamics studies and cell interaction studies. Furthermore, we envision that these platforms can be used with different cell types and for other biological applications, such as spheroid formation and single cell analysis, …