Medicine and Health Sciences Commons^™

Itaconate Transporter Slc13a3 Impairs Tumor Immunity Via Endowing Ferroptosis Resistance, Heng Lin, Kole Tison, Yuheng Du, Paul Kirchhoff, Chan Kim, Weichao Wang, Hannah Yang, Michael Pitter, Jiali Yu, Peng Liao, Jiajia Zhou, Linda Vatan, Sara Grove, Shuang Wei, Thomas Vigil, Yatrik M Shah, Richard Mortensen, Ilona Kryczek, Lana Garmire, Jwala P Sivaccumar, Ashwin Kumar Ramesh, Ningyan Zhang, Zhiqiang An, Shaomeng Wang, Weiping Zou

The Brown Foundation: Institute of Molecular Medicine

Immune checkpoint blockade (ICB) triggers tumor ferroptosis. However, most patients are unresponsive to ICB. Tumors might evade ferroptosis in the tumor microenvironment (TME). Here, we discover SLC13A3 is an itaconate transporter in tumor cells and endows tumor ferroptosis resistance, diminishing tumor immunity and ICB efficacy. Mechanistically, tumor cells uptake itaconate via SLC13A3 from tumor-associated macrophages (TAMs), thereby activating the NRF2-SLC7A11 pathway and escaping from immune-mediated ferroptosis. Structural modeling and molecular docking analysis identify a functional inhibitor for SLC13A3 (SLC13A3i). Deletion of ACOD1 (an essential enzyme for itaconate synthesis) in macrophages, genetic ablation of SLC13A3 in tumors, or treatment with SLC13A3i …

Bowen’S Disease Mimicking Lichen Simplex Chronicus In A 56-Year-Old Filipino Man: A Case Report, Cynthia Angela Karim, Elisabeth Ryan, Reynaldo Ugalde

Journal of General - Procedural Dermatology and Venereology Indonesia

Background: Bowen's disease (BD) is a chronic skin condition presenting clinically as erythematous plaques with scales on sun-exposed areas. BD is generally regarded as a squamous cell carcinoma (SCC) in situ. In contrast, lichen simplex chronicus (LSC), also known as neurodermatitis, is a chronic skin disorder characterized by extreme pruritus. In LSC, lichenified plaques form primarily on accessible body parts due to repeated scratching or rubbing.

Case Illustration: A 56-year-old male presented with a solitary chronic plaque with a central ulcer and erosions on his left upper thigh. Dermoscopy findings were glomerular vessels and a scaly surface, which are …

Association Of Unmet Social Needs With Disease-Related Outcomes In Pediatric Patients With Sickle Cell Disease, Molly Sonenklar, Sarah Marks, Cerelia Donald, Cecelia Valrie, Wally Smith, India Sisler

School of Medicine Faculty Publications

Background: Social determinants of health (SDoH) are socioeconomic factors that influence health and well-being, though when unmet can greatly contribute to health disparities. Individuals with sickle cell disease (SCD) are at increased risk of mortality, disability, and healthcare utilization. However, there are limited data linking specific social needs with disease outcomes in this population. Therefore, we sought to identify the unmet needs in one institution and their association with healthcare utilization. Methods: Children with SCD and their guardians answered demographic and SDoH questionnaires during routine Sickle Cell Clinic appointments. We defined an unmet social need as any positive answer to …

Appropriateness Of Ambulatory Antibiotic Prescribing In South Carolina, 2012–2017, Pamela Bailey, Majdi N. Al-Hasan, Julie Royer, Max Habicht, Julie Ann Justo, P. Brandon Bookstaver Pharm.D., Sharon Weissman, Hana R. Winders

Faculty Publications

This population-based cohort study examines the appropriateness of antibiotic prescribing in South Carolina via aggregated pharmacy claims data matched with diagnosis codes from medical claims. Inappropriate antibiotic prescribing decreased from 30.2% in 2012 to 22.6% in 2017 (P < 0.001) and was more common in adults >40 years old.

Adipocyte-Derived Small Extracellular Vesicles Exacerbate Diabetic Ischemic Heart Injury By Promoting Oxidative Stress And Mitochondrial-Mediated Cardiomyocyte Apoptosis, Lu Gan, Jianli Zhao, Peng Yao, Theodore A. Christopher, Bernard Lopez, Wayne B. Lau, Walter Koch, Erhe Gao, Xin-Liang Ma, Yajing Wang

Department of Emergency Medicine Faculty Papers

BACKGROUND: Diabetes increases ischemic heart injury via incompletely understood mechanisms. We recently reported that diabetic adipocytes-derived small extracellular vesicles (sEV) exacerbate myocardial reperfusion (MI/R) injury by promoting cardiomyocyte apoptosis. Combining in vitro mechanistic investigation and in vivo proof-concept demonstration, we determined the underlying molecular mechanism responsible for diabetic sEV-induced cardiomyocyte apoptosis after MI/R.

METHODS AND RESULTS: Adult mice were fed a high-fat diet (HFD) for 12 weeks. sEV were isolated from plasma or epididymal adipose tissue. HFD significantly increased the number and size of plasma- and adipocyte-derived sEV. Intramyocardial injection of an equal number of diabetic plasma sEV in nondiabetic …

¿Qué Dijo El Médico?: Creating A Procedural Preparation Booklet For Spanish-Speaking Patients And Families In The Pediatric Hospital Setting, Caitlyn Bockbrader

Honors Projects

The purpose of my Honors Project is to demonstrate the need for and creation of a resource that supports Spanish-speaking children’s ability to cope through commonly stressful medical procedures. In developing this project, I conducted a literature review on the efficacy of preparation booklets in lessening pre-procedural anxiety, cultural considerations for the Hispanic community, and current barriers to equitable language access in healthcare settings. My final product was designed to answer the following question: How can the psychosocial outcomes of Spanish-speaking children in U.S. hospitals be improved through the use of Child Life procedural preparation materials? In answering this question, …

Physical Fitness And Nutrition In Collegiate Musical Theater Programs, Alexis Ochi, Amy Morgan Ph.D, Mary-Jon Ludy Ph.D.

Honors Projects

BACKGROUND: Athleticism and physical fitness are often not the first characteristics one identifies when visualizing someone in musical theater. Watching just one performance can make it clear that dancing, acting, running, all while singing and keeping near perfect pitch requires a theater actor to be extremely fit. As well as fitness, nutrition considerations and injury prevention should also be considered due to the high physiological demand placed on these performers. Minimal research has been conducted regarding the fitness and nutritional habits of musical theater performers. PURPOSE: This study investigated the prevalence of discussions about fitness and nutrition in collegiate musical …

Wearable Surface Electromyography System To Predict Freeze Of Gait In Parkinson's Disease Patients., Anna Moore, Jinxing Li, Christopher H Contag, Luke J Currano, Connor O Pyles, David A Hinkle, Vivek Shinde Patil

Neuroscience Articles

Freezing of gait (FOG) is a disabling yet poorly understood paroxysmal gait disorder affecting the vast majority of patients with Parkinson's disease (PD) as they reach advanced stages of the disorder. Falling is one of the most disabling consequences of a FOG episode; it often results in injury and a future fear of falling, leading to diminished social engagement, a reduction in general fitness, loss of independence, and degradation of overall quality of life. Currently, there is no robust or reliable treatment against FOG in PD. In the absence of reliable and effective treatment for Parkinson's disease, alleviating the consequences …

Factors Associated With Receipt Of Tobacco Treatment Integrated With Nurse Navigation In A Centralized Lung Cancer Screening Program At An Urban Academic Medical Center, Christine S. Shusted, Sarah Mukhtar, James Lee, Brooke Ruane, Emily Muse, Lawrence Emeigh-Mcbride, Eboni Gatson-Anderson, Gregory C. Kane, Kuang-Yi Wen, Hee-Soon Juon, Julie A. Barta

Division of Pulmonary, Allergy, and Critical Care Medicine Faculty Papers

PURPOSE: Optimal approaches for tobacco treatment counseling among individuals who currently smoke and are undergoing shared decision-making (SDM) and lung cancer screening (LCS) are unknown. The objective of this cross-sectional study was to determine the rate of reported interest in tobacco treatment counseling and pharmacotherapy among individuals who currently smoke and are receiving integrated nurse navigation for LCS and tobacco treatment in a centralized LCS Program.

METHODS: We identified individuals undergoing SDM through our centralized LCS Program between March 2021 and March 2022. The LCS Program tobacco treatment protocol includes counseling both in-person and via telephone during SDM, LCS results …

Comparison Of Quality Of Life, Anxiety, And Depression Levels Based On Acne Vulgaris Severity, Annisaul Husna, Satya Wydya Yenny, Desmawati Desmawati, Gardenia Akhyar, Masrul Masrul, Yulistini Yulistini

Journal of General - Procedural Dermatology and Venereology Indonesia

Background: Acne vulgaris is a chronic inflammation of the pilosebaceous unit. Although acne vulgaris is not fatal, it can cause psychological effects. This study aimed to compare the quality of life, anxiety, and depression levels based on the severity of acne vulgaris among medical students at Andalas University.

Methods: This was an observational-analytic study with a cross-sectional design. It was conducted from May to June 2023. The population included the third-grade students of the Medical Study Program of the Faculty of Medicine, Andalas University, who had acne vulgaris. The respondents were 70 people, recruited with consecutive sampling techniques. This research …

Dermoscopy And 30% Potassium Hydroxide Direct Examination As Diagnostic Tools For Distal Lateral Subungual Onychomycosis, Evangelina Lumban Gaol, Eliza Miranda, Sondang Pandjaitan Sirait

Journal of General - Procedural Dermatology and Venereology Indonesia

Background: Culture and histopathological examination are gold standards for diagnosing onychomycosis. However, these examinations are subject to several limitations. Dermoscopy has the potential to become a promising diagnostic tool for onychomycosis. Direct examination of potassium hydroxide (KOH) also has advantages similar to dermoscopy. Knowing the accuracy of both examinations can help clinical decision-making. Our objectives are to assess the diagnostic value of dermoscopy, KOH examination, and their combination for distal lateral subungual onychomycosis compared to culture or histopathology.

Methods: This was a cross-sectional diagnostic study of nails with suspected distal lateral subungual onychomycosis. Sixty nails were included in this study. …

The Spectrum Of Cutaneous Manifestations In Covid-19 Patients At Dr. Cipto Mangunkusumo Hospital, Sandra Widaty, Sri Linuwih Susetyo Wardhani Menaldi, Eliza Miranda, Melani Marissa, Hanny Nilasari, Thong Felicia Melinda, Emilina Faradila Cornain

Journal of General - Procedural Dermatology and Venereology Indonesia

Background: Cutaneous manifestations are identified in 0.2%–20% of COVID-19 cases, both in adult and pediatric patients. They can occur before, simultaneously with, or after the systemic symptoms of COVID-19. This study aims to analyze the association between cutaneous manifestations, severity, and outcomes of patients with COVID-19.

Methods: This observational study was conducted from April 2020 to April 2021 in Dr. Cipto Mangunkusumo Hospital, a referral center for COVID-19 in Jakarta, Indonesia. Medical records were used to collect demographic, clinical, and laboratory data.

Results: A total of 0.61% (31/5070) of COVID-19 cases showed cutaneous manifestations, consisting of maculopapular (80.6%), vesicular …

Effectiveness Of Mental Health Interventions For Adults With Cancer In The Us: An Integrative Review, Danielle Lee, Chelsea Ferguson

Master's Projects

The purpose of this review was to synthesize existing literature on the effects of mental health interventions for adult cancer patients following their initial diagnosis and to consolidate findings related to interventions addressing mental health conditions in this population. With the growing number of adults diagnosed with cancer, further research and the development of tailored mental health interventions are crucial to improving health outcomes.

A systematic search was conducted using PubMed, CINAHL, Medline Complete, and Health Source Nursing databases between January 2024 and February 2024. To ensure rigor, Toronto and Remington’s (2020) integrative review process was applied. The Johns Hopkins …

An Exploration Of Telemedicine Application Service Quality And Its Relevance With Patient Satisfaction, Victoria Olguin

Honors Program Theses and Research Projects

When the world experienced a pandemic due to the SARS-CoV-virus (COVID-19) in 2019, a lockdown quickly took place and travel restrictions were applied, placing a barrier to in- person healthcare access. This made the healthcare settings practice alternate ways in delivering healthcare that did not necessarily have to be in-person. The evolvement of a remote setting took place, and the development and usage of telemedicine quickly accelerated. This form of healthcare has transformed the way care is delivered to patients, with many key elements shaping their experience and outcomes. Now that the COVID-19 pandemic has ended, the practice of telemedicine …

The Relationship Between Religious Coping Styles And Eating Disorder Risk In Christians In A University Setting, Meghan E. Aubuchon

Honors Theses

Existing research on eating disorders and religion is beginning to gain momentum in the present day, but research is lacking on the topic of religious coping and eating disorder risk. This study aimed to investigate the relationship between two known styles of religious coping—positive and negative—and eating disorder risk in University of Mississippi students, faculty, and staff who identify as Christian. A survey consisting of basic demographic questions, the EAT-26, and the Brief RCOPE was distributed via emails sent by the office of IREP to students, faculty, and staff at the University of Mississippi. A total of 330 participants were …

Oncologic Outcomes In Patients With Localized, Primary Head And Neck Synovial Sarcoma, Riddhi R Patel, Vancheswaran Gopalakrishnan, Behrang Amini, Alexander J Lazar, Patrick P Lin, Robert S Benjamin, Andrew J Bishop, Ryan P Goepfert, Dejka M Araujo

Faculty, Staff and Student Publications

Background: this study aims to evaluate the survival outcomes of patients suffering from head and neck synovial sarcoma (HNSS), especially in relation to patients with a localized disease at diagnosis.

Methods: this retrospective chart review includes 57 patients diagnosed with primary HNSS between 1981 and 2020 who presented with a localized disease at diagnosis. Overall survival (OS) from diagnosis, local recurrence-free survival (LRFS), and metastasis-free survival (MFS) from the end of the primary tumor treatment are estimated. The Kaplan-Meier method, the log-rank test, and the Cox proportional hazards regression are used.

Results: the 5-year OS, LRFS, and MFS are estimated …

Rare Breast Cancers Review, Bowen Song, Harnoor Singh

Faculty, Staff and Student Publications

Background/objectives: Breast cancer is one of the most common malignancies in women, with rare subtypes presenting unique clinical challenges. This review provides a comprehensive analysis of rare breast cancers, including both epithelial and non-epithelial subtypes, and explores their epidemiology, pathology, prognosis, and treatment approaches.

Methods: A systematic review was conducted focusing on recent advancements in the treatment of rare breast cancer subtypes. Articles were selected based on criteria emphasizing studies from the past five years, with older foundational studies included where necessary. The analysis incorporated molecular profiling, clinical trials, and advancements in targeted and immunotherapies, where possible.

Results: Rare epithelial …

Automated Segmentation Of Mri White Matter Hyperintensities In 8421 Patients With Acute Ischemic Stroke, Hosung Kim, Wi-Sun Ryu, Dawid Schellingerhout, Jonghyeok Park, Jinyong Chung, Sang-Wuk Jeong, Dong-Seok Gwak, Beom Joon Kim, Joon-Tae Kim, Keun-Sik Hong, Kyung Bok Lee, Tai Hwan Park, Jong-Moo Park, Kyusik Kang, Yong-Jin Cho, Byung-Chul Lee, Kyung-Ho Yu, Mi Sun Oh, Soo Joo Lee, Jae-Kwan Cha, Dae-Hyun Kim, Jun Lee, Man Seok Park, Hee-Joon Bae, Dong-Eog Kim

Faculty, Staff and Student Publications

Background and purpose: To date, only a few small studies have attempted deep learning-based automatic segmentation of white matter hyperintensity (WMH) lesions in patients with cerebral infarction; this issue is complicated because stroke-related lesions can obscure WMH borders. We developed and validated deep learning algorithms to segment WMH lesions accurately in patients with cerebral infarction using multisite data sets involving 8421 patients with acute ischemic stroke.

Materials and methods: We included 8421 patients with stroke from 9 centers in Korea. 2D UNet and squeeze-and-excitation (SE)-UNet models were trained using 2408 FLAIR MRIs from 3 hospitals and validated using 6013 FLAIR …

Additional Expression Of T-Cell Engager In Clinically Tested Oncolytic Adeno-Immunotherapy Redirects Tumor-Infiltrated, Irrelevant T Cells Against Cancer Cells To Enhance Antitumor Immunity, Daisuke Morita, Amanda Rosewell Shaw, Greyson Biegert, Caroline Porter, Mae Woods, Spyridoula Vasileiou, Bora Lim, Masataka Suzuki

Faculty, Staff and Student Publications

Background: Oncolytic adenoviruses (OAds) are the most clinically tested viral vectors for solid tumors. However, most clinically tested "Armed" OAds show limited antitumor effects in patients with various solid tumors even with increased dosages and multiple injections. We developed a binary oncolytic/helper-dependent adenovirus system (CAdVEC), in which tumors are coinfected with an OAd and a non-replicating helper-dependent Ad (HDAd). We recently demonstrated that a single low-dose CAdVEC expressing interleukin-12, programmed death-ligand 1 blocker, and HSV thymidine kinase safety switch (CAdTrio) induces significant antitumor effects in patients, including complete response. Similar to previous OAd studies, all patients primarily amplified Ad-specific T …

Clinical And Radiologic Findings In Children With Anomalous Pontine Cranial Nerves, Karen K Moeller, Brandon H Tran, Thierry A G M Huisman, Nilesh K Desai, Marcia K Kukreja, Rajan P Patel, Uma S Ramaswamy, Carol Liu, Stephen F Kralik

Faculty, Staff and Students Publications

We retrospectively reviewed the clinical and radiologic findings in 17 children with an aberrant cisternal cranial nerve 7 (CN7), and found that these patients had additional anomalies involving other pontine cranial nerves (CNs). The hallmark imaging feature identified in all patients was an aberrant cisternal segment of an enlarged-appearing CN7. The abnormal nerve coursed anteriorly toward the Gasserian ganglion, where it fanned out toward the internal auditory canal, Meckel cave, or both. This finding was accompanied by a small cisternal CN5, which often had a lateral bowed appearance. CN5 and CN7 were abnormally close to each other. Meckel's cave appeared …

Anodal Transcranial Direct Current Stimulation (Tdcs) Modulates Quadriceps Motor Cortex Inhibition And Facilitation During Rehabilitation Following Anterior Cruciate Ligament (Acl) Reconstruction: A Triple-Blind, Randomised Controlled Proof Of Concept Trial, Myles Calder Murphy, Colin Sylvester, Casey Whife, Peter D'Alessandro, Ebonie Kendra Rio, Ann Maree Vallence

Research outputs 2022 to 2026

Objectives Following anterior cruciate ligament reconstruction (ACLR), maladaptive changes occur in the motor cortex representation of the quadriceps, evidenced by increases in intracortical inhibition and facilitation. The primary objective of this proof-of-concept study was to determine if anodal transcranial direct current stimulation (tDCS) can alter quadriceps intracortical inhibition and facilitation in an early-ACLR population after 6 weeks of application during exercise. Methods We performed a randomised, triple-blind controlled trial for proof of concept comparing anodal-tDCS to sham-tDCS following ACLR. Anodal-tDCS or sham-tDCS was delivered to the primary motor cortex for 20 min, three times per week, for 6 weeks from …

Delivering Improved Survivorship Care For People Affected By Advanced Or Metastatic Cancer, Julia Lai-Kwon, Sarah Heynemann, Nicolas Hart, Raymond J. Chan, Thomas Smith, Andrea L. Smith, Larissa Nekhlyudov, Michael Jefford

Research outputs 2022 to 2026

No abstract provided.

Interventions For Aboriginal And Torres Strait Islander People With Type 2 Diabetes That Modify Its Management And Cardiometabolic Risk Factors: A Systematic Review, Ciying Tan, Zoe Williams, Mohammad Ashraful Islam, Ray Kelly, Tuguy Esgin, Elif I. Ekinci

Research outputs 2022 to 2026

Objectives: To review studies of interventions for reducing the impact of type 2 diabetes in Aboriginal and Torres Strait Islander people. The primary aim was to review and summarise the characteristics and findings of the interventions. The secondary aims were to assess their effects on diabetes and cardiometabolic risk factors, and the proportions of people with type 2 diabetes who achieved therapeutic targets with each intervention. Study design: We searched eight electronic databases for publications of studies including Aboriginal or Torres Strait Islander people aged 15 years or older with diagnoses of type 2 diabetes, describing one or more diabetes …

Transdisciplinary Collaboration Of Healthcare Professionals And The Emotional Connectedness With Working With Patients Coping With Death And Dying, Princess Nguyen, Lindsay Gietzen

Pacific Journal of Health

Abstract

Purpose: This study explores how an integrated approach to care for patients and families by transdisciplinary healthcare teams provides opportunities for a patient-centered care approach to support quality of life throughout one's chronic illness and at the end-of-life. Discussions and conversations about impending death or dying are often challenging and a complex task for healthcare professionals in whatever settings. While there is an understanding of aspects of collaborative care and transitions and processes to support patients and families about how to cope and communicate about death and dying, it is essential to recognize how best to support end-of-life care …

Disparities In The Pregnancy Experience: A Multi-Case Analysis, Tiffany C. Thomas, Lindsay Gietzen

Pacific Journal of Health

Racial disparities in maternal healthcare continue to be a significant issue, with Black women experiencing worse outcomes compared to White women. Despite improvements in healthcare, Black women are still 2.5 times more likely to die from pregnancy-related causes, and they face higher rates of preterm births, low birth weight, and insufficient prenatal care. This paper examines the experiences of three African American women from high socioeconomic backgrounds, focusing on where these negative experiences are most common throughout pregnancy, childbirth, and postnatal care. The study uses the Pregnancy and Maternity Care Patients' Experiences Questionnaire (PreMaPEQ) to explore different stages of care, …

The Influence Of Socioeconomic Factors On The Leadership Styles Of Healthcare Leaders, Regina Viney, Lindsay Gietzen

Pacific Journal of Health

Socioeconomic factors have been shown to influence opportunities, decisions, motivation, and outcomes in all areas of life. Yet very little is known about how socioeconomic factors influence the leadership styles of healthcare leaders. Five nurse leaders from different backgrounds were interviewed to identify if those Leaders believed that their Socioeconomic factors played a role in the leadership style, they were comfortable emulating. Semi-structured interviews were conducted with healthcare leaders in San Joaquin County. A total of five participants (three female, two male) were interviewed for this study. Participants were asked questions about their leadership style and socioeconomic factors. These questions …

Do New Mothers In Their First-Year Postpartum Feel Prepared To Return To Daily Occupations?, Kim Rose, Jane L. Donat, Cedrica Durham, Bianca Haddock, Danyl Litumbe, Julia Otoski, Katsia Renaud

Occupational Therapy Annual Research Colloquium

Purpose: Our research aimed to explore if new mothers felt prepared to return to their daily lives and the role occupational therapy could have in helping prepare women to transition into their new role as mothers. Previous literature has found that limits to occupational engagement and occupational balance have adversely affected the well-being of new mothers.

Methods: This sequential, exploratory mixed methods study employed the RedCap survey platform to collect demographic information, information regarding women’s birth experience and to obtain consent. Twenty-two women who filled out the survey met the inclusion criteria, and ten were selected to proceed with the …

Understanding Provider Competency In Gender Affirming Care, Vanessa Castle, Lindsay Gietzen

Pacific Journal of Health

Lesbian, gay, bisexual, transgender, queer/questioning, intersex, and asexual plus (LGBTQ+) individuals, especially transgender patients, face many healthcare disparities. It is extremely important for them to have access to high-quality healthcare, particularly Gender Affirming Care (GAC), to support their well-being and mental health. However, many healthcare providers, including clinical social workers, NPs, PAs, and MDs lack proper training to offer GAC. The current training opportunities available are often basic and lack comprehensive, detailed information. To reduce healthcare barriers for the transgender and gender-diverse community, we must have comprehensive training on GAC available for providers so they can treat their patients with …

Benefits Of Meditation Practices In Alleviating Posttraumatic Stress Disorder (Ptsd) Symptoms For Individuals Already In Therapy, Jeanette G. Mann, Lindsay Gietzen

Pacific Journal of Health

Abstract

This study examines the impact of adding and incorporating a meditation practice into a treatment plan for Posttraumatic Stress Disorder (PTSD) for three adult patients at a Federally Qualified Health Center (FQHC). The cases involved included patients with persistent PTSD symptoms who had experienced limited improvement with standard therapies. Data from Electronic Health Records and clinician notes showed that adding an eight-week meditation practice enhanced emotional regulation and reduced anxiety. In all three cases, the meditation practice increased the benefits of the traditional therapy. This helped the patients have greater control over their PTSD symptoms and improve their quality …

Purity And Characterize The Killer Proteins Produced By Saccharomyces Cerevisiae Strains, Hiba Hadi Aldabbagh, Najwa Ibrahim Al-Barhawee, Faten Noori Mula Abed

BioMed Visions Journal

Identified killer toxin proteins from Saccharomyces cerevisiae and characterized by HPLC , Therefore, electrophoresis was performed to extract and characterize the protein on a polyacrylamide gel by method SDS – PAGE,The protein bands appeared on the gel, and determined was k28 which secreted by S.cerevisiae depend on molecular weight, which was estimated at 5 kilodalton. It was used in subsequent studies, and the confirmed this toxin by using HPLC which compared with the standard sample. Using bioinformatics In the analysis of the results, were identified the atomic structure and the crystalline shape of the three-dimensional protein by Pymol analysis program …