Medicine and Health Sciences Commons^™

Reducing The Effects Of Oxidative And Nitrosative Stress On Viability Of The Broiler Blood Cells When Exposed To High Ambient Temperatures By Using Tannic Acid, Jaroon Wandee, Piyarat Srinontong, Worapol Aengwanich

The Thai Journal of Veterinary Medicine

The study aimed to investigate the effect of tannic acid (TA) on total antioxidant power (FRAP), malondialdehyde (MDA), hydrogen peroxide (H2O2), nitric oxide (NO), and the viability of broiler blood cells (BBC) exposed to high ambient temperatures. The BBC from a broiler chicken was exposed to temperatures ranging from 41.5°C to 46°C and incubated with TA at concentrations of 0 (the positive control group, TPCG), 5, 10, 15, and 20 μg/mL. Results showed that MDA, H2O2, and NO levels of the control group (TCG) which BBC was maintained at 41.5°C were significantly lower than TPCG. The viability of TPCG was …

Biomechanical Comparison Of Transosseous Four-Strand Tendon Suture Patterns For Repair Of Ruptured Distal Common Calcaneal Tendon: An Ex Vivo Study In Dogs, Tae-Hwan Ahn, Min-Yeong Lee, Jaekyoung Lee, Hwi-Yool Kim

The Thai Journal of Veterinary Medicine

There is no golden standard method of suturing for distal common calcaneal tendon rupture in dogs. Thus this study aimed to evaluate and compare the mechanical characteristics of three distinct transosseous suture (TOS) repair techniques, which were specifically developed to treat ruptured distal common calcaneal tendons and to identify golden standard suturing method. The study was performed using 30 hind limbs obtained from 15 canine cadavers with weights ranging between 7.9 kg and 11.4 kg. The repair methods using modified Becker suture (group 1), modified Krackow suture (group 2), and modified double-Tsuge suture (group 3) were randomly assigned. Each group …

Efficacy Of In-House Nanosilver Polyurethane Hydrogel Dressing On Second-Degree Burn Wounds In A Porcine Model, Pasakorn Brikshavana, Tapanat Songkasupa, Pleansaung Vichit, Onrampa Supsombon, Arunrode Nilrattanaprasert, Theerayuth Kaewamatawong

The Thai Journal of Veterinary Medicine

The aim of this study was to investigate the effects of hydrogel containing nanosilver particles on a second-degree burn wound in a porcine model. The second degree burn wound was performed using 1 cm in diameter, on 100 °C heated cylindrical brass bar applied to the dorsum of pig skin. Burn wounds were randomly divided into three groups of wound dressings (WDs) including commercial polyurethane hydrogel (Cutinova Hydro®, Gr. A), in-house hydrogel (Gr. B) and in-house nanosilver hydrogel (Gr. C). The wound contraction rate and hyperemic zone were measured and investigated at 3, 7, 14 and 21 days after dressing. …

In-House Polydimethylsiloxane Microfluidic Device On Single Cell Trapping And Culturing Of Leukemia Cell Line: Comparison Between Conventional And Conventional With Centrifugal Methods, Alongkorn Pimpin, Sudchaya Bhanpattanakul, Tepparit Wongpakham, Chomkwan Tawatcharaporn, Thanawit Siha-Umphai, Mookdapa Sinsiriphan, Atikan Chalermchuang, Tewan Tongmanee, Attawut Thanomsridetchai, Thammawit Suwannaphan, Witsaroot Sripumkhaie, Pattaraluck Pattamang, Mayuree Chanasakulniyom, Praprudee Piyaviriyakul, Wutthinun Jeamsaksiri, Achariya Sailasuta, Theerawat Tharasanit, Theerayuth Kaewamatawong

The Thai Journal of Veterinary Medicine

To develop a suitable model to study biological and characterization of Cancer stem cells (CSCs), single cell trapping and culturing using microfluidic techniques have become a high-through put system that can manage and control microenvironment and practical intracellular analysis. The aim of this study was to develop and validate a microfluidic device for single cell trapping and culturing using the leukemia cell line as a cell model. Comparison between conventional and conventional with centrifugal methods was also analyzed. To compare trapping efficacy between these 2 methods, leukemia cell concentration of 1.5×106 cells/ml was loaded with a flow rate of 0.25 …

Molecular Characterization And Antibiogram Of Riemerella Anatipestifer In Ducklings In Upper Egypt, Mohamed Sabry Ahmed, Amal Abd El-Ghani Batahi, Ahmed Ibrahim Ahmed, Nabila Osman

The Thai Journal of Veterinary Medicine

Riemerella anatipestifer infection represents a great threat to the duck industry worldwide and it is considered the major cause of death in the backyard rearing system of ducks in Egypt. The disease is characterized by diarrhea, respiratory signs and loss of weight. The present study was conducted during the period between July 2019 and January 2021 to examine the prevalence rate, the antibiogram patterns of the different antibiotics and the pathogenicity of R. anatipestifer in Qena province. One-hundred samples were collected from diseased and freshly dead backyard ducklings. A tentative characterization of R. anatipestifer was done depending on the phenotypic …

The Benefits Of Dexmedetomidine For Pain Mitigation After Cautery Disbudding In Calves, Tsung-Ching Liu, Chen-Fei Chiang, Thomas Chia-Tang Ho, Jacky Peng-Wen Chan

The Thai Journal of Veterinary Medicine

The purpose of this study was to determine the effect of dexmedetomidine (DEX) alone on pain mitigation following cautery disbudding in calves. A total of 21 calves were randomly divided into three groups: sham-disbudded control with placebo (S, n = 8); hot-iron disbudded after placebo (D, n = 6); hot-iron disbudded after 0.01 mg/kg of DEX (DeD, n = 7). The pain-related responses measured included serum cortisol levels, struggling scores, pain-related behaviors and other physiological values. The results indicated that the struggling scores, the cortisol concentrations from +10 to +60 min after disbudding and the area under curve of cortisol …

Interlocking Nail Stabilization Technique For Long Bone Fractures In Calves, Mustafa Arican, Semih Altan, Kurtuluş Parlak, Fahrettin Alkan

The Thai Journal of Veterinary Medicine

The aim of this study was to report the clinical and radiographic outcome from using an interlocking nail (ILN) technique for the stabilization of long bone fractures in 15 calves. Forty-eight calves with fractures were presented to the large animal hospital. Fifteen calves of different breeds, age, sex and weight with front or hind limb fractures were considered suitable for ILN application. These included proximal or distal diaphyseal transverse and oblique fractures of the humerus, radius, femur and tibia. The third generation of six, eight, nine and 10mm diameter interlocking nails were used in this study. Interlocking pins were used …

The Effect Of Intranasal Medetomidine/Ketamine And Diazepam/Ketamine Combinations On Tear Production And Intraocular Pressure In Dogs, Osman Bulut, Rahime Yaygingul

The Thai Journal of Veterinary Medicine

To determine the effect of intranasal medetomidine/ketamine and diazepam/ketamine combinations on tear production and intraocular pressure in dogs. The animal material included 28 dogs of different breeds, ages, weights and genders that were brought to the surgery department of the Adnan Menderes University, Faculty of Veterinary Medicine for simple anesthesia indications but were otherwise healthy ocularly. In a crossover design, the dogs were randomly assigned to 4 groups of 7 dogs. Animals in the first group were given 50 μg/kg of medetomidine and 10 mg/kg of ketamine intramuscularly (MEK-IM). The second group of animals was given 50 μg/kg of medetomidine …

Productivity Of Young Broilers Fed Phyllanthus Niruri Linn And Melaleuca Cajuput Leaf Meal As A Phytogenic Feed Additive, Listya Purnamasari

The Thai Journal of Veterinary Medicine

Feed additives from medicinal plants can be used as alternative antibiotics to optimize productivity, growth, and health of livestock. Medicinal plants Phyllanthus niruri Linn (Meniran) and Melaleuca cajuput (Kajuput) as alternative growth promoters contain antioxidants and antimicrobials to improve the quality of animal products. The aim of the study was to evaluate the effect of medicinal plant leaf meals as phytogenic feed additives on broilers’ productivity and internal organ parameters. For 21 days fed, 80 1-day-old Loughmann broilers (MB202) unsex were distributed in 4 treatments with 4 pens per treatment and 5 birds per pen. The dietary treatments were: T1 …

A Study On Stress-Induced By Anaesthesia And Surgery Training Using Live Animals In A Veterinary School, Krittee Dejyong, Jutapoln Sunghan, Nantarat Chutijiratthitikan, Chonnakarn Jatchavala

The Thai Journal of Veterinary Medicine

The veterinary curriculum includes an anesthesia and surgery course that utilizes live animal laboratories and euthanasia, potentially causing stress in students. We used a validated psychology questionnaire to assess their stress levels, confirming that live animal laboratories induce significant stress. Forty-two fourth year veterinary students, including individuals who identified as male, female, and LGBTQ, and who held religious beliefs in Buddhism and Islam, volunteered to participate in the study. The study had received ethical approval from the Human Research Ethics Committee at Prince of Songkla University. After series of stress questionnaires before and after Veterinary surgery and anaesthesia class, female …

An Anatomical, Histological, And Immunohistochemical Investigation On The Intestines Of The Eurasian Lynx (Lynx Lynx), Şükran Yedi̇el Aras, Gülseren Kırbaş Doğan, Ebru Karadağ Sarı

The Thai Journal of Veterinary Medicine

In this study, it was aimed to perform an anatomical, histological, and immunohistochemical investigation of the intestines of the lynx. After performing macro-anatomical measurements of the tissues obtained from the small and large intestines of the lynx which was brought to the Kafkas University Veterinary Faculty Clinics and Wild Life Preservation and Rehabilitation Center for treatment but could not be saved despite all interventions, the routine histological procedure was followed. Triple staining was applied to examine the general histological structure of intestinal tissues, and PAS staining was applied for identifying goblet cells. Somatostatin distribution was examined immunohistochemically. The lengths and …

Characteristics Of Extended-Spectrum Beta-Lactamase (Esbl)- Producing Escherichia Coli Associated With Antimicrobial Use In Captive Wild Animals, Montira Yossapol, Jinnapat Imidom, Kannarun Sakhorn, Pimpatcha Thanomwat, Sarawut Sonrit, Siraphop Labmala, Tarid Purisotayo, Natapol Pumipuntu, Justice Opare Odoi, Adithep Konputtar

The Thai Journal of Veterinary Medicine

Antimicrobial agents are a cause of the increase in antimicrobial-resistant (AMR) bacteria. We investigated the prevalence and characteristics of extended-spectrum beta-lactamase (ESBL)-producing bacteria in captive wild animals before and after exposure to antimicrobial agents. Sixty-six fecal samples were collected from captive animals (spotted deer, Rusa deer, geese, chickens, rabbits, and guinea pigs) at the Mahasarakham University Mini Zoo in Thailand on three sampling occasions: October 2021, January 2022, and April 2022. Using MacConkey agar supplemented with cefotaxime, ESBL-producing Escherichia coli (ESBL-Ec) was isolated and subjected to antimicrobial susceptibility tests and genetic analysis. The results showed a high prevalence of ESBL-Ec …

Protective Immunity Of The Pore-Forming Domains Of Actinobacillus Pleuropneumoniae Apx Toxins In A Mouse Model, Kamonpun Chuekwon, Li-Ting Cheng, Chun-Yen Chu

The Thai Journal of Veterinary Medicine

The hemolytic/leukolytic toxins ApxI, II, and III play a central role in the pathogenicity of Actinobacillus pleuropneumoniae and may serve as effective vaccine antigens. However, since these toxins are large in size, we aimed to determine whether only the pore-forming domain of the toxins may be sufficient for protective immunity. Mice were vaccinated with the pore-forming domains for immune response analysis and challenge test. Significant antibody response was observed for the ApxI and II vaccine groups. For cellular immune response, CD4⁺ and CD8⁺ T cell expansion was observed for ApxI. Pro-inflammatory cytokine (IL-1b, IL-6) and T_H2-type …

Discovery Of Chicken Proventricular Necrosis Virus From Transmissible Viral Proventriculitis Outbreak In Broilers, Thailand, ์Nantaporn Wandee, Preeda Lertwatchrasarakul, Thaweesak Songserm

The Thai Journal of Veterinary Medicine

Transmissible viral proventriculitis (TVP) is an infectious disease-causing retarded growth in chickens. In Thailand, many stunted chickens have been found on commercial broiler farms since 2017. Significant post-mortem lesions are found only in the proventriculus. This study aims to investigate the causative agents of TVP outbreaks focusing on chicken proventricular necrosis virus (CPNV), the possible significant cause of TVP. CPNV was reproduced in specific pathogen-free (SPF) chickens. Affected chickens that have undergone proventriculitis are CPNV-positive by RT-PCR and nucleotide sequencing followed by phylogenetic analysis. The result showed that proventriculitis could be experimentally reproduced by inoculation of proventricular homogenate obtained from …

Effect Of Rosmarinic Acid On Dog Semen Quality After Freeze-Drying By Applying Freeze-Drying Machines For Vaccine Production, Janjira Phavaphutanon, Phannipa Phungsombun, Kornchai Kornkaewrat, Sudtisa Laopiem, Wilairat Chumsing, Pattra Moonjit

The Thai Journal of Veterinary Medicine

Freeze-drying (FD) is a sperm preservation method that eliminates the need for liquid nitrogen. However, the FD process can expose sperm to free radicals and temperature variations. This study aimed to determine the impact of rosmarinic acid (RA) as an antioxidant, initial sperm motility, and storage temperature on the DNA integrity of freeze-dried dog sperm. Forty-four ejaculates from 15 dogs of various breeds were divided into control (FD extender) and RA (FD extender with RA) groups, further categorized by motility (above or below 70%). After FD, samples were stored for three months at room temperature and 4 °C. Sperm quality …

Relationship Of Hyperlipidemia And Gallbladder Sludge Scores In Dogs With Hyperadrenocorticism, Jayeop Jo, Dohee Lee, Taesik Yun, Yoonhoi Koo, Yeon Chae, Dongwoo Chang, Byeong-Teck Kang, Mhan-Pyo Yang, Kyung-Duk Min, Hakhyun Kim

The Thai Journal of Veterinary Medicine

Hyperadrenocorticism (HAC) and hyperlipidemia have been identified as the causes of cholestatic disease in dogs. However, the possibility of direct association of HAC with a cholestatic disease or secondary hyperlipidemia-induced cholestatic disease in dogs with HAC has not been elucidated. This study aimed to evaluate the differences in serum triglyceride (TG), total cholesterol (TC) and basal cortisol (BC) concentrations based on the gallbladder (GB) sludge score in dogs with HAC. This study was a retrospective cohort study. In total, 36 dogs with pituitary-dependent hyperadrenocorticism (PDH) were divided into six groups (GB scores 0–5) based on the ultrasonographic evaluation of the …

Dmt: Mechanisms, Psychological And Physiological Effects, And Its Dual Role As A Drug Of Abuse And Potential Health Benefit, Kayla Yarbrough, Solymar Santiago Bonilla, Garrett Evins, Jean Colón, Brandon Kitchens

College of Pharmacy Student Research

N, N-Dimethyltryptamine (DMT) is a potent indolethylamine hallucinogen classified as a Schedule I substance, indicating a high potential for abuse and no accepted medical use. Despite this, DMT's unique pharmacological profile, characterized by its interaction with serotonin receptors, particularly 5-HT2A, and its ability to induce profound alterations in perception, emotion, and cognition, has spurred interest in its potential therapeutic applications. This paper explores DMT's mechanisms of action, its psychological and physiological effects, and the risks and side effects associated with its use. While DMT can cause vivid hallucinations, emotional responses ranging from euphoria to existential dread, and various physiological changes, …

Medical School Watercooler Newsletter - August 15, 2024, Lindsay Hughes

Watercooler Newsletter

This is the August 15, 2024 edition of the Frederick P. Whiddon College of Medicine’s newsletter - Watercooler.

Contents Include

• Escobar joins the MCI as medical oncologist and hematologist
• USA Health welcomes new urogynecologist

• Medical students recognized for excellence in summer research

• BMS graduate students join lab 'matches': Part 2

• Goodloe follows family path to emergency medicine

Measuring Compassion In Virtual Primary Care Encounters: Report On A Pilot Study, Bridget L. Ryan, Megann Dong

Family Medicine Publications

The Virtual Compassionate Primary Care Scale (VCPC) was developed as part of a larger study whose aim was to establish specific actions and activities that should be included within virtual primary care encounters to ensure compassionate care. We pilot tested patient and family physician 9-item versions of the VCPC to assess the degree of compassion within patient-family physician virtual encounters.

The pilot testing took place within individual interviews with patients and family physicians (not patient-family physician dyads) where we asked them to reflect on a recent specific encounter receiving [for patients] or providing [for family physicians] virtual care. Seventeen patients …

Outpatient Administration Of Car T-Cell Therapies Using A Strategy Of No Remote Monitoring And Early Crs Intervention, Fateeha Furqan, Vineel Bhatlapenumarthi, Binod Dhakal, Timothy S. Fenske, Faiqa Farrukh, Walter Longo, Othman Akhtar, Anita D'Souza, Marcelo Pasquini, Guru Subramanian Guru Murthy, Lyndsey Runaas, Sameem Abedin, Meera Mohan, Nirav N. Shah, Mehdi Hamadani

Abington Jefferson Health Papers

Recent studies demonstrating the feasibility of outpatient chimeric antigen receptor (CAR)-modified T-cell therapy administration are either restricted to CARs with 41BB costimulatory domains or use intensive at-home monitoring. We report outcomes of outpatient administration of all commercially available CD19- and B-cell maturation antigen (BCMA)-directed CAR T-cell therapy using a strategy of no remote at-home monitoring and an early cytokine release syndrome (CRS) intervention strategy. Patients with hematologic malignancies who received CAR T-cell therapy in the outpatient setting during 2022 to 2023 were included. Patients were seen daily in the cancer center day hospital for the first 7 to 10 days …

Epigenetic Mechanisms Differentially Regulate Blood Pressure And Renal Dysfunction In Male And Female Npr1 Haplotype Mice, Prerna Kumar, Kandasamy Neelamegam, Chandramohan Ramasamy, Ramachandran Samivel, Huijing Xia, Daniel R. Kapusta, Kailash N. Pandey

School of Graduate Studies Faculty Publications

We determined the epigenetic mechanisms regulating mean arterial pressure (MAP) and renal dysfunction in guanylyl cyclase/natriuretic peptide receptor-A (GC-A/NPRA) gene-targeted mice. The Npr1 (encoding NPRA) gene-targeted mice were treated with class 1 specific histone deacetylase inhibitor (HDACi) mocetinostat (MGCD) to determine the epigenetic changes in a sex-specific manner. Adult male and female Npr1 haplotype (1-copy; Npr1+/−), wild-type (2-copy; Npr1+/+), and gene-duplicated heterozygous (3-copy; Npr1++/+) mice were intraperitoneally injected with MGCD (2 mg/kg) for 14 days. BP, renal function, histopathology, and epigenetic changes were measured. One-copy male mice showed significantly increased MAP, renal dysfunction, and fibrosis than 2-copy and 3-copy mice. …

In Vitro Antibacterial, Antibiofilm Activities, And Phytochemical Properties Of Posidonia Oceanica (L) Delile: An Endemic Mediterranean Seagrass, Ertugrul Ozbil, Mehmet Ilktac, Sultan Ogmen, Ovgu Isbilen, Jesus M Duran Ramirez, Jana Gomez, Jennifer N Walker, Ender Volkan

Faculty, Staff and Student Publications

In the antibiotic resistance era, utilizing understudied sources for novel antimicrobials or antivirulence agents can provide new advances against antimicrobial resistant pathogens. In this study, we aimed to investigate antibacterial and antibiofilm activities of Posidonia oceanica (L.) Delile against Enterococcus faecalis ATCC 29212, Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922 and Klebsiella pneumoniae ATCC 700603 and several S. aureus clinical isolates obtained from medical devices, including patient urinary catheters and breast implant infections, with varying antibiotic recalcitrance profiles. The ethanolic and methanolic extracts from P. oceanica rhizome exhibited significant antibacterial activity against E. faecalis and S. aureus, as well …

Hematopoietic Growth Factors Regulate The Entry Of Monocytes Into The Adult Brain Via Chemokine Receptor Ccr5, Xuefang Sophie Ren, Junchi He, Songruo Li, Heng Hu, Michele Kyle, Shinichi Kohsaka, Li-Ru Zhao

Faculty, Staff and Student Publications

Monocytes are circulating macrophage precursors generated from bone marrow hematopoietic stem cells. In adults, monocytes continuously replenish cerebral border-associated macrophages under physiological conditions. Monocytes also rapidly infiltrate the brain in pathological settings. The mechanisms of recruiting monocyte-derived macrophages into the brain under pathological conditions have been extensively studied. However, it remains unclear how monocytes enter the brain to renew border-associated macrophages under physiological conditions. Using both in vitro and in vivo approaches, this study reveals that a combination of two hematopoietic growth factors, stem cell factor (SCF) and granulocyte colony-stimulating factor (G-CSF), complementarily and synergistically enhances the adhesion of monocytes …

The Impact Of Face Masks On Face-To-Face Neural Tracking Of Speech: Auditory And Visual Obstacles, M. Fantoni, A. Federici, I. Camponogara, G. Handjaras, A. Martinelli, E. Bednaya, E. Ricciardi, F. Pavani, D. Bottari

All Works

Face masks provide fundamental protection against the transmission of respiratory viruses but hamper communication. We estimated auditory and visual obstacles generated by face masks on communication by measuring the neural tracking of speech. To this end, we recorded the EEG while participants were exposed to naturalistic audio-visual speech, embedded in 5-talker noise, in three contexts: (i) no-mask (audio-visual information was fully available), (ii) virtual mask (occluded lips, but intact audio), and (iii) real mask (occluded lips and degraded audio). Neural tracking of lip movements and of the sound envelope of speech was measured through backward modeling, that is, by reconstructing …

Manualized Occupational Therapy Intervention For Children And Adolescents With Post-Traumatic Stress Disorder, Adam Mcmahon

Student Capstone Papers

Post-traumatic stress disorder (PTSD) in children can cause physiological and psychological reactions that can lead to impaired executive functioning and self-regulation skills that interfere with occupational engagement. Occupational Therapy (OT) utilizes an occupational perspective to treat children with PTSD. However, there is an absence of a manualized OT intervention in the literature that incorporates an occupation-based approach, utilizes graded occupation as the primary intervention, and tracks treatment progress and outcomes. The purpose of this capstone project was to create a manualized OT intervention protocol for OT practitioners to treat children with PTSD guided by the model of human occupation and …

The Association Between Long Non-Coding Rnas And Alzheimer’S Disease, Carson M. Black, Anneliesse A. Braden, Samia Nasim, Manish Tripathi, Jianfeng Xiao, Mohammad Moshahid Khan

School of Medicine Publications

Neurodegeneration occurs naturally as humans age, but the presence of additional pathogenic mechanisms yields harmful and consequential effects on the brain. Alzheimer’s disease (AD), the most common form of dementia, is a composite of such factors. Despite extensive research to identify the exact causes of AD, therapeutic approaches for treating the disease continue to be ineffective, indicating important gaps in our understanding of disease mechanisms. Long non-coding RNAs (lncRNAs) are an endogenous class of regulatory RNA transcripts longer than 200 nucleotides, involved in various regulatory networks, whose dysregulation is evident in several neural and extraneural diseases. LncRNAs are ubiquitously expressed …

Redoma: Bayesian Random-Effects Dose-Optimization Meta-Analysis Using Spike-And-Slab Priors, Cheng-Han Yang, Evan Kwiatkowski, J Jack Lee, Ruitao Lin

Faculty, Staff and Student Publications

The rise of cutting-edge precision cancer treatments has led to a growing significance of the optimal biological dose (OBD) in modern oncology trials. These trials now prioritize the consideration of both toxicity and efficacy simultaneously when determining the most desirable dosage for treatment. Traditional approaches in early-phase oncology trials have conventionally relied on the assumption of a monotone relationship between treatment efficacy and dosage. However, this assumption may not hold valid for novel oncology therapies. In reality, the dose-efficacy curve of such treatments may reach a plateau at a specific dose, posing challenges for conventional methods in accurately identifying the …

Factors Associated With Elevated Sars-Cov-2 Immune Response In Children And Adolescents, Sarah E Messiah, Rhiana Abbas, Emma Bergqvist, Harold W Kohl, Michael D Swartz, Yashar Talebi, Rachit Sabharwal, Haoting Han, Melissa A Valerio-Shewmaker, Stacia M Desantis, Ashraf Yaseen, Henal A Gandhi, Ximena Flandes Amavisca, Jessica A Ross, Lindsay N Padilla, Michael O Gonzalez, Leqing Wu, Mark A Silberman, David Lakey, Jennifer A Shuford, Stephen J Pont, Eric Boerwinkle

Faculty, Staff and Student Publications

BACKGROUND: Understanding the distinct immunologic responses to SARS-CoV-2 infection among pediatric populations is pivotal in navigating the COVID-19 pandemic and informing future public health strategies. This study aimed to identify factors associated with heightened antibody responses in children and adolescents to identify potential unique immune dynamics in this population.

METHODS: Data collected between July and December 2023 from the Texas Coronavirus Antibody REsponse Survey (Texas CARES), a statewide prospective population-based antibody survey among 1-to-19-year-old participants, were analyzed. Each participant had the following data available for analysis: (1) Roche Elecsys® Anti-SARS-CoV-2 Immunoassay for Nucleocapsid protein antibodies (Roche N-test), (2) qualitative and …

Impact Of Gene-By-Trauma Interaction In Mdd-Related Multimorbidity Clusters, Sarah Bonk, Nora Eszlari, Kevin Kirchner, Andras Gezsi, Linda Garvert, Mikko Kuokkanen, Isaac Cano, Hans J. Grabe, Peter Antal, Gabriella Juhasz, Sandra Van Der Auwera

School of Medicine Publications

Background

Major depressive disorder (MDD) is considerably heterogeneous in terms of comorbidities, which may hamper the disentanglement of its biological mechanism. In a previous study, we classified the lifetime trajectories of MDD-related multimorbidities into seven distinct clusters, each characterized by unique genetic and environmental risk-factor profiles. The current objective was to investigate genome-wide gene-by-environment (G × E) interactions with childhood trauma burden, within the context of these clusters.

Methods

We analyzed 77,519 participants and 6,266,189 single-nucleotide polymorphisms (SNPs) of the UK Biobank database. Childhood trauma burden was assessed using the Childhood Trauma Screener (CTS). For each cluster, Plink 2.0 was …

Affinity Purification Mass Spectrometry Characterisation Of The Interactome Of Receptor Tyrosine Kinase Proline-Rich Motifs In Cancer, Christopher M Jones, Arndt Rohwedder, Kin Man Suen, Safoura Zahed Mohajerani, Antonio N Calabrese, Sabine Knipp, Mark T Bedford, John E Ladbury

Faculty, Staff and Student Publications

Receptor tyrosine kinase (RTK) overexpression is linked to the development and progression of multiple cancers. RTKs are classically considered to initiate cytoplasmic signalling pathways via ligand-induced tyrosine phosphorylation, however recent evidence points to a second tier of signalling contingent on interactions mediated by the proline-rich motif (PRM) regions of non-activated RTKs. The presence of PRMs on the C-termini of >40 % of all RTKs and the abundance of PRM-binding proteins encoded by the human genome suggests that there is likely to be a large number of previously unexplored interactions which add to the RTK intracellular interactome. Here, we explore the …