Medicine and Health Sciences Commons^™

Cardiovascular, Autonomic Symptoms And Quality Of Life In Children With Hypermobile Ehlers-Danlos Syndrome., Amanda K. Hertel, William R. Black, Ashley Lytch, Emily Cramer, Lindsey Malloy-Walton, Jordan T. Jones

Manuscripts, Articles, Book Chapters and Other Papers

OBJECTIVES: Hypermobile Ehlers-Danlos syndrome is a connective tissue disorder characterized by joint hypermobility and other systemic manifestations. Cardiovascular, autonomic symptoms and dysautonomia are frequently reported in adults with hypermobile Ehlers-Danlos syndrome and have been shown to have a negative impact on quality of life. However, there is scant literature on autonomic symptoms in pediatric patients with hypermobile Ehlers-Danlos syndrome. This study aims to characterize cardiovascular symptoms and diagnoses in pediatric patients with hypermobile Ehlers-Danlos syndrome and evaluate the impact of autonomic symptoms on quality of life.

METHODS: As part of a longitudinal study, a consecutive sample of 70 patients with …

A Feasibility Study Of A Music Enrichment Program On Relative Reinforcing Value Of Food And Home Environmental Enrichment Among Families Of Low Socioeconomic Status., Kai Ling Kong, Amy R. Smith, Brenda Salley, Deanna Hanson-Abromeit, Hideko Engel, Catherine Serwatka

Manuscripts, Articles, Book Chapters and Other Papers

BACKGROUND: Emerging evidence suggests that low socioeconomic status (SES) home environments may play a role by promoting excess energy intake through a lack of access to non-food reinforcers. Because of the deleterious effects of SES-related disparities on child health and development, feasible and culturally acceptable interventions are urgently needed. Community-based music enrichment programs may be an ideal intervention strategy.

METHODS: In collaboration with a local non-profit organization and music studio, we conducted a pilot randomized controlled trial to assess the effects of a music enrichment program versus a play date control in a group of 9-24-month-old healthy infants (N = …

Long Covid Among Undocumented Latino Immigrant Populations In The Emergency Department., Karen P. Reyes, Zubaid Rafique, Brian Chinnock, Vijaya Arun Kumar, Michael Gottlieb, Kristin L. Rising, Robert M. Rodriguez

Department of Emergency Medicine Faculty Papers

Although many investigators have examined post–COVID-19 condition (long COVID) and its effects in the general population, to our knowledge, no studies have investigated its effect on one of the largest underserved US populations: undocumented Latino immigrants.¹ This group comprises 7% of the US population and has high rates of uninsurance, limited access to primary care, and language barriers when seeking health care.^2-4 Anti-immigrant political rhetoric and immigrants’ fear of discovery of their undocumented status when accessing health care make evaluation of this population more challenging.⁵ We leveraged emergency departments (EDs) as the primary health care …

Enhancing Human Aging Proteins Detection Based On Bio-Structural Deep Clustering Network, Sondos M. Hammad, Mohamed Talaat Saidahmed, Elsayed A. Sallam, Reda Elbasiony

Journal of Engineering Research

Aging is a major risk factor for numerous chronic diseases and declines in physical and cognitive functions, making it a critical focus of research. Identifying biomarkers that signify biological aging is crucial for developing interventions that can potentially delay age-related diseases. Among these biomarkers, human aging proteins are of significant interest. However, laboratory experiments to discover aging proteins are complex, costly, and time-consuming. Often focusing on single proteins at a time. To address this challenge, we used a clustering algorithm to detect potential human aging proteins. This approach aims to aid biologists in their experimental efforts. Deep clustering, particularly simultaneous …

Evaluation Of Virtual Distance Learning And Teaching In The Architectural Design Studio Experience Of Professors And Students Of The College Of Architecture And Planning - Qassim University - Kingdom Of Saudi Arabia., Abdulaziz Ibrahim Alhrabi

Journal of Engineering Research

The paper deals with the evaluation of distance learning of the architectural design studio during the Corona pandemic, and the extent to which distance learning and teaching were achieved between the professor and the student. The paper aims to share and evaluate the experience of distance learning and teaching of the architectural design studio, in the College of Architecture and Planning at Qassim University. The research problem is that the Corona virus crisis generates many ideas for educational programs and media, but there is a deficiency in knowledge of many programs that achieve the continuity of the educational process remotely, …

Nano Topology And Decision Making In Medical Applications, Samir Mukhtar, Mohamed Shokry, Manar Omran

Journal of Engineering Research

Nano Topology is one of the essential topics that receive special attention from some athletes in the field of General Topology, Operations Research, and Computer Science, because it has a vital role in the generalizing most of the various mathematical concepts. Recently, many efforts have been made to study many types of Nano Topology, as the previous studies lacked real applications in Engineering, Medicine, Pharmacy, and Social Sciences. In this paper, we present some different applications of these studies. The paper is divided into two parts: Firstly, we study the theory of The Nano Topology and investigate its relation with …

New Operation Defined Over Dual-Hesitant Fuzzy Set And Its Application In Diagnostics In Medicine, Manar Mohamed Omran, Reham Abdel-Aziz Abo-Khadra

Journal of Engineering Research

In recent decades, several types of sets, such as fuzzy sets, interval-valued fuzzy sets, intuitionistic fuzzy sets, interval-valued intuitionistic fuzzy sets, type 2 fuzzy sets, type n fuzzy sets, and hesitant fuzzy sets, have been introduced and investigated widely. In this paper, we propose dual hesitant fuzzy sets (DHFSs), which encompass fuzzy sets, intuitionistic fuzzy sets, hesitant fuzzy sets, and fuzzy multi-sets as special cases. Then we investigate the basic operations and properties of DHFSs. We also discuss the relationships among the sets mentioned above, and then propose an extension principle of DHFSs. Additionally, we give an example to illustrate …

Optimal Site Layout Planning For Construction Projects Over Waterways, Rana M. Azzam, Nashwa Mohamed Yossef Prof, Adel I. Eldosouky Prof

Journal of Engineering Research

Construction site layout planning is an important part of a project's success. An effective construction layout plan can enhance the performance of the construction process by improving productivity, ensuring safety, and reducing costs. Given the importance of bridges over a waterway and the lack of interest in previous research for planning a construction site over a waterway. This paper concentrates on the problems of site planning for such projects, including the problem of limited site space. The study indicated that constructing an offshore terminal is one of the primary requirements for projects with limited space being carried out on a …

Decision-Making In Diagnosing Heart Failure Problems Using Dual Hesitant Fuzzy Sets, Manar Mohamed Omran, Reham Abdel-Aziz Abo-Khadra

Journal of Engineering Research

In recent decades, several types of sets, such as fuzzy sets, interval-valued fuzzy sets, intuitionistic fuzzy sets, interval-valued intuitionistic fuzzy sets, type 2 fuzzy sets, type n fuzzy sets, and hesitant fuzzy sets, have been introduced and investigated widely. In this paper, we propose dual hesitant fuzzy sets (DHFSs), which encompass fuzzy sets, intuitionistic fuzzy sets, hesitant fuzzy sets, and fuzzy multi-sets as special cases. Then we investigate the basic operations and properties of DHFSs. We also discuss the relationships among the sets mentioned above, and then propose an extension principle of DHFSs. Additionally, we give an example to illustrate …

Developing Paths In Areas Of Distinguished Value Between Preservation And Extinction (A Case Study Of The Railway Axis In The Maadi Area), Kamal Mahmoud Elgabalawy

Journal of Engineering Research

Abstract: proportion of the place, and thus the loss of the distinctive general character of the area, which leads to its extinction. These areas are of distinguished value, with all the thought and culture they carry of society over time. Among the areas classified as having distinguished value is the Maadi suburb, which is located in the south of Cairo Governorate, where boundaries and foundations for preserving them have been set through the Central Administration for Studies, Research, and Policies of the National Coordination Organization. The Ministry of Culture, approved by the Supreme Council for Planning and Urban Development by …

Review Of Wood Drying Technologies, Mohamed Salah Elmetwaly, Lotfy Hassan Rabie Saker, Mohamed Sameh Salem

Journal of Engineering Research

Abstract- This article provides a comprehensive review of drying optimization in data centers by examining the limitations of drying methods and discussing advances proposed by scientists through whom we can provide a comparison between different drying systems with an emphasis on improving energy efficiency and thermal performance. Wood drying under atmospheric pressure has many advantages, including the ability to dry at lower temperatures (by reducing the likelihood of some drying defects), significantly reduced drying times, color preservation, increased energy efficiency, better control of volatile organic compound emissions, and the ability to dry very large cross sections. Previous studies have achieved …

"Towards A Methodology For Studying The Impact Of Integrating Motion Systems In The External Envelope Of A Building On Natural Lighting Inside The Building– (An Applied Study On A Government School In Greater Cairo)", Nashwa Youssif

Journal of Engineering Research

يدرك المصمم ما للعناصر البيئية ولا سيما الإضاءة الطبيعية من دور فاعل في الوصول إلى تصميم ناجح يحقق أفضل أداء إستخدامي، فالإضاءة الطبيعية تذهب إلى صميم عمل المعماري فيستعين بها للإيحاء بشكل الفراغات ولونها وملمس سطوحها.

وهناك العديد من الأساليب الحديثة التي تساعد المعماري علي إتخاذ قراراته التصميمية الخاصة بتصميم المبنى وكيفية الإستفادة من الإضاءة الطبيعية داخل المباني، وهذه الأساليب تهدف إلى زيادة إحترام البيئة وتفترض تحسينات في النظم البيئية من العزل الحراري وإستغلال مصادر الطاقة الطبيعية والإهتمام بنظم الإضاءة الطبيعية والمواد البنائية.

وتعد تقنية دمج الأنظمة الحركية بالغلاف الخارجي للمبنى مع الإستفادة من الإضاءة الطبيعية داخل هذا …

Challenges In Paediatric Xenotransplantation: Ethical Components Requiring Distinct Attention In Children And Obligations To Patients And Society, Anthony Merlocco, Daniel J Hurst

Rowan-Virtua School of Osteopathic Medicine Departmental Research

The transplantation of non-human organs into humans, or xenotransplantation (XTx), has recently garnered new attention and is being developed to help address the problem of organ scarcity in transplantation. Ethical issues surrounding XTx have been studied since initial interest arose decades ago and have experienced renewed discussion in the literature. However, the distinct and relevant differences when applied to children has largely been overlooked with few groups attending to the concerns that XTx in children raises. In this paper, we explore ethical challenges to be expected in paediatric XTx, in particular exploring organ sizing concerns, infectious risks, psychological burdens, and …

Racial Residential Segregation And Covid-19 Health Outcomes: Evidence From The State Of Georgia, Palak Patel, Simon Medcalfe, Catherine P. Slade

Journal of the Georgia Public Health Association

ABSTRACT

Background:

According to the Georgia Department of Public Health (GDPH), the state of Georgia reported 563,658 cumulative COVID-19 cases and 9,845 total deaths in 2020. Decades of research on racial disparities in health outcomes suggest we should not be surprised with the disproportionate number of cases, hospital visits, and deaths of non-white and Black Georgia residents. Racial disparities in health are often defined by a Social Determinants of Health (SDOH) model. One understudied SDOH is racial residential segregation. In this study we explore the relationship between racial residential segregation and COVID-19 health outcomes. Our paper addresses critical challenges for …

Understanding The Role Of Perceived Racial Discrimination On Adolescent Mental Health During The Covid-19 Pandemic, Jill V. Klosky, Delia Lang, Briana Woods-Jaeger, Julie Gazmararian

Journal of the Georgia Public Health Association

Abstract

Background: Adolescent mental health declined during the COVID-19 pandemic. Racial discrimination, which may negatively affect adolescent mental health, has increased. This study sought to understand whether pre-pandemic reports of racial discrimination predicted changes in adolescent depression, anxiety, and self-esteem from before to during the COVID-19 pandemic.

Methods: Two online surveys were administered at two public high schools in semi-rural, north-central Georgia; one in Spring 2020, just before the closing of public schools due to COVID-19; the other to the same students in Fall 2020.

Results: Most participants were White (62%) and the majority (88%), reported the same or better …

93rd Annual Georgia Public Health Association Meeting And Conference Report, Jimmie H. Smith Jr, Angela Peden, Patrina L. Bowles

Journal of the Georgia Public Health Association

The 93^rd Annual Meeting and Conference of the Georgia Public Health Association (GPHA) was held in Jekyll Island, Georgia, at the Jekyll Island Convention Center, on May 3-5, 2023. The goal of the 2023 Georgia Public Health Association Annual Meeting and Conference was to assemble emerging public health professionals, current practitioners, academicians, governing entities, and community stakeholders for educational opportunities and networking to support the vision and mission of GPHA.

The theme for the 2023 Georgia Public Health Association Annual Meeting and Conference theme was selected by the GPHA Executive Board during a meeting in the early fall of …

Adding The Third Dimension: 3d Convolutional Neural Network Diagnosis Of Temporal Lobe Epilepsy, Erik Kaestner, Reihaneh Hassanzadeh, Ezequiel Gleichgerrcht, Kyle Hasenstab, Rebecca W. Roth, Allen Chang, Theodor Rüber, Kathryn A. Davis, Patricia Dugan, Ruben Kuzniecky, Julius Fridriksson Ph.D., Alexandra Parashos, Anto I. Bagić, Daniel L. Drane, Simon S. Keller, Vince D. Calhoun, Anees Abrol, Leonardo Bonilha, Carrie R. Mcdonald

Faculty Publications

Convolutional neural networks (CNN) show great promise for translating decades of research on structural abnormalities in temporal lobe epilepsy into clinical practice. Three-dimensional CNNs typically outperform two-dimensional CNNs in medical imaging. Here we explore for the first time whether a three-dimensional CNN outperforms a two-dimensional CNN for identifying temporal lobe epilepsy-specific features on MRI. Using 1178 T1-weighted images (589 temporal lobe epilepsy, 589 healthy controls) from 12 surgical centres, we trained 3D and 2D CNNs for temporal lobe epilepsy versus healthy control classification, using feature visualization to identify important regions. The 3D CNN was compared to the 2D model and …

Lowering The Burden: Shorter Versions Of The Program Sustainability Assessment Tool (Psat) And Clinical Sustainability Assessment Tool (Csat), Sara Malone, Kim Prewitt, Virginia Mckay, Luke Zabotka, Caren Bacon, Douglas A Luke

2020-Current year OA Pubs

BACKGROUND: Although significant advances have been made in the conceptualization of sustainability, having pragmatic, psychometrically valid tools remains a need within the field. Our previous work has developed frameworks and tools to assess both program sustainability and clinical sustainability capacity. This work presents new, psychometrically tested short versions of the Program Sustainability Assessment Tool (PSAT) and the Clinical Sustainability Assessment Tool (CSAT).

METHODS: These methods were conducted in identical, parallel processes for the CSAT and PSAT. Previously collected data for these instruments was obtained across a variety of settings, contexts, and participants. We first conducted testing to determine cronbach's alpha …

Recent Progress In Modeling And Treating Diabetes Using Stem Cell-Derived Islets, Marlie M Maestas, Maggie H Bui, Jeffrey R Millman

2020-Current year OA Pubs

Stem cell-derived islets (SC-islets) offer the potential to be an unlimited source of cells for disease modeling and the treatment of diabetes. SC-islets can be genetically modified, treated with chemical compounds, or differentiated from patient derived stem cells to model diabetes. These models provide insights into disease pathogenesis and vulnerabilities that may be targeted to provide treatment. SC-islets themselves are also being investigated as a cell therapy for diabetes. However, the transplantation process is imperfect; side effects from immunosuppressant use have reduced SC-islet therapeutic potential. Alternative methods to this include encapsulation, use of immunomodulating molecules, and genetic modification of SC-islets. …

Rethinking Our Future: Describing And Enhancing The Impacts Of Dissemination And Implementation Science For Cancer Prevention And Control, Vianca C. Soulette, Karen M. Emmons, Douglas A. Luke, Peg Allen, Bobbi J. Carothers, Ross C. Brownson

2020-Current year OA Pubs

No abstract provided.

Molecular Characterization Of Sterol C4-Methyl Oxidase In Leishmania Major, Yu Ning, Somrita Basu, Fong-Fu Hsu, Mei Feng, Michael Zhuo Wang, Kai Zhang

2020-Current year OA Pubs

Sterol biosynthesis requires the oxidative removal of two methyl groups from the C-4 position by sterol C-4-demethylase and one methyl group from the C-14 position by sterol C-14-demethylase. In

Clinical And Functional Studies Of Mtor Variants In Smith-Kingsmore Syndrome Reveal Deficits Of Circadian Rhythm And Sleep-Wake Behavior, Andrew C Liu, Yang Shen, Carolyn R Serbinski, Hongzhi He, Destino Roman, Mehari Endale, Lindsey Aschbacher-Smith, Katherine A King, Jorge L Granadillo, Isabel López, Darcy A Krueger, Thomas J Dye, David F Smith, John B Hogenesch, Carlos E Prada

2020-Current year OA Pubs

Heterozygous de novo or inherited gain-of-function mutations in the MTOR gene cause Smith-Kingsmore syndrome (SKS). SKS is a rare autosomal dominant condition, and individuals with SKS display macrocephaly/megalencephaly, developmental delay, intellectual disability, and seizures. A few dozen individuals are reported in the literature. Here, we report a cohort of 28 individuals with SKS that represent nine MTOR pathogenic variants. We conducted a detailed natural history study and found pathophysiological deficits among individuals with SKS in addition to the common neurodevelopmental symptoms. These symptoms include sleep-wake disturbance, hyperphagia, and hyperactivity, indicative of homeostatic imbalance. To characterize these variants, we developed cell …

Aligning The World Health Organization's (Who) Package Of Interventions For Rehabilitation For Cancer With The Mission Of The International Psycho-Oncology Society's: Promoting Psychosocial Care For All People Affected By Cancer, Christina Signorelli, Nicolas H. Hart, Louise Mullen, Larissa Nekhlyudov, Luzia Travado, Wendy W.T. Lam, Melissa Henry, Csaba László Dégi, Darren Haywood, Michael Jefford

Research outputs 2022 to 2026

The number of people living with and beyond cancer continues to increase worldwide, bringing significant attention to their rehabilitation needs. Globally, psychosocial services are largely inadequate, with many cancer survivors experiencing unmet psychosocial needs. The World Health Organization (WHO) recognizes rehabilitation as an essential component of universal health coverage to prevent disease-related conditions, while also improving physical and mental functioning and overall well-being. The Package of Interventions for Rehabilitation (PIR) was developed by the WHO to address the global need for rehabilitation across 20 conditions with high prevalence and high levels of associated disability, including cancer. Many aspects of the …

An Epiq Mass Shelter Training For Public Health Nurses In Georgia To Improve Core Competencies In Disaster Preparedness: A Pilot Study, Ashlie L. Pullen, Sonya Shinhoster

Journal of the Georgia Public Health Association

Background: Research shows that nurses are often ill-prepared for disaster response. A survey conducted by the Georgia Department of Public Health Office of Nursing in April of 2021, demonstrated that 45% of the public health nurses surveyed had never deployed to a mass congregate shelter, and 43% reported they had no formal training related to emergency preparedness or mass sheltering. The purpose of this pilot study was to measure public health nurses’ improvement in familiarity with emergency preparedness and disaster response core competencies as defined by the Emergency Preparedness Information Questionnaire (EPIQ) after a focused educational program and tabletop workshop. …

Multivariable Mendelian Randomization With Incomplete Measurements On The Exposure Variables In The Hispanic Community Health Study/Study Of Latinos, Yilun Li, Kin Yau Wong, Annie Green Howard, Penny Gordon-Larsen, Heather M Highland, Mariaelisa Graff, Kari E North, Carolina G Downie, Christy L Avery, Bing Yu, Kristin L Young, Victoria L Buchanan, Robert Kaplan, Lifang Hou, Brian Thomas Joyce, Qibin Qi, Tamar Sofer, Jee-Young Moon, Dan-Yu Lin

Faculty, Staff and Student Publications

Multivariable Mendelian randomization allows simultaneous estimation of direct causal effects of multiple exposure variables on an outcome. When the exposure variables of interest are quantitative omic features, obtaining complete data can be economically and technically challenging: the measurement cost is high, and the measurement devices may have inherent detection limits. In this paper, we propose a valid and efficient method to handle unmeasured and undetectable values of the exposure variables in a one-sample multivariable Mendelian randomization analysis with individual-level data. We estimate the direct causal effects with maximum likelihood estimation and develop an expectation-maximization algorithm to compute the estimators. We …

Impact Of Age On Pharmacogenomics And Treatment Outcomes Of B-Cell Acute Lymphoblastic Leukemia, Satoshi Yoshimura, Zhenhua Li, Yoshihiro Gocho, Wenjian Yang, Kristine R Crews, Shawn H R Lee, Kathryn G Roberts, Charles G Mullighan, Mary V Relling, Jiyang Yu, Allen E J Yeoh, Mignon L Loh, Caner Saygin, Mark R Litzow, Sima Jeha, Seth E Karol, Hiroto Inaba, Ching-Hon Pui, Marina Konopleva, Nitin Jain, Wendy Stock, Elisabeth Paietta, Elias Jabbour, Steven M Kornblau, William E Evans, Jun J Yang

Faculty, Staff and Student Publications

Purpose: Acute lymphoblastic leukemia (ALL) can occur across all age groups, with a strikingly higher cure rate in children compared with adults. However, the pharmacological basis of age-related differences in ALL treatment response remains unclear.

Methods: Studying 767 children and 309 adults with newly diagnosed B-cell ALL enrolled on frontline trials at St Jude Children's Research Hospital, MD Anderson Cancer Center, the Alliance for Clinical Trials in Oncology, and the ECOG-ACRIN Cancer Research Group, we determined the ex vivo sensitivity of leukemia cells to 21 drugs. Twenty-three ALL molecular subtypes were identified using RNA sequencing. We systematically characterized the associations …

Larp1 Haploinsufficiency Is Associated With An Autosomal Dominant Neurodevelopmental Disorder, James Chettle, Raymond J Louie, Olivia Larner, Robert Best, Kevin Chen, Josephine Morris, Zinaida Dedeic, Anna Childers, R Curtis Rogers, Barbara R Dupont, Cindy Skinner, Sébastien Küry, Kevin Uguen, Marc Planes, Danielle Monteil, Megan Li, Aviva Eliyahu, Lior Greenbaum, Nofar Mor, Thomas Besnard, Bertrand Isidor, Benjamin Cogné, Alyssa Blesson, Anne Comi, Ingrid M Wentzensen, Blake Vuocolo, Seema R Lalani, Roberta Sierra, Lori Berry, Kent Carter, Stephan J Sanders, Sarah P Blagden

Faculty, Staff and Students Publications

Autism spectrum disorder (ASD) is a neurodevelopmental disorder (NDD) that affects approximately 4% of males and 1% of females in the United States. While causes of ASD are multi-factorial, single rare genetic variants contribute to around 20% of cases. Here, we report a case series of seven unrelated probands (6 males, 1 female) with ASD or another variable NDD phenotype attributed to de novo heterozygous loss of function or missense variants in the gene LARP1 (La ribonucleoprotein 1). LARP1 encodes an RNA-binding protein that post-transcriptionally regulates the stability and translation of thousands of mRNAs, including those regulating cellular metabolism and …

Rapid Evolution Of Mitochondrion-Related Genes In Haplodiploid Arthropods, Yiyuan Li, Gregg W C Thomas, Stephen Richards, Robert M Waterhouse, Xin Zhou, Michael E Pfrender

Faculty, Staff and Students Publications

Background: Mitochondrial genes and nuclear genes cooperate closely to maintain the functions of mitochondria, especially in the oxidative phosphorylation (OXPHOS) pathway. However, mitochondrial genes among arthropod lineages have dramatic evolutionary rate differences. Haplodiploid arthropods often show fast-evolving mitochondrial genes. One hypothesis predicts that the small effective population size of haplodiploid species could enhance the effect of genetic drift leading to higher substitution rates in mitochondrial and nuclear genes. Alternatively, positive selection or compensatory changes in nuclear OXPHOS genes could lead to the fast-evolving mitochondrial genes. However, due to the limited number of arthropod genomes, the rates of evolution for nuclear …

Churning The Tides Of Care: When Nurse Turnover Makes Waves In Patient Access To Primary Care, Kelley Arredondo, Ashley M Hughes, Houston F Lester, Trang N D Pham, Laura A Petersen, Lechauncy Woodard, Richard Sorelle, Cheng Rebecca Jiang, Frederick L Oswald, Daniel R Murphy, Hilary N Touchett, Joshua Hamer, Sylvia J Hysong

Faculty, Staff and Students Publications

BACKGROUND: Team-based primary care (PC) enhances the quality of and access to health care. The Veterans Health Administration (VHA) implements team-based care through Patient Aligned Care Teams (PACTs), consisting of four core members: a primary care provider, registered nurse (RN) care manager, licensed vocational nurse, and scheduling clerk. RNs play a central role: they coordinate patient care, manage operational needs, and serve as a patient point of contact. Currently, it is not known how varying levels of RN staffing on primary care teams impact patient outcomes.

OBJECTIVE: This study aims to empirically assess how the stability of RN staffing within …

Systematic Perturbations Of Setd2, Nsd1, Nsd2, Nsd3, And Ash1l Reveal Their Distinct Contributions To H3k36 Methylation, Gerry A Shipman, Reinnier Padilla, Cynthia Horth, Bo Hu, Eric Bareke, Francisca N Vitorino, Joanna M Gongora, Benjamin A Garcia, Chao Lu, Jacek Majewski

2020-Current year OA Pubs

BACKGROUND: Methylation of histone 3 lysine 36 (H3K36me) has emerged as an essential epigenetic component for the faithful regulation of gene expression. Despite its importance in development and disease, how the molecular agents collectively shape the H3K36me landscape is unclear.

RESULTS: We use mouse mesenchymal stem cells to perturb the H3K36me methyltransferases (K36MTs) and infer the activities of the five most prominent enzymes: SETD2, NSD1, NSD2, NSD3, and ASH1L. We find that H3K36me2 is the most abundant of the three methylation states and is predominantly deposited at intergenic regions by NSD1, and partly by NSD2. In contrast, H3K36me1/3 are most …