Medicine and Health Sciences Commons^™

Hypoxia Increases Atx Expression By Histone Crotonylation In A Hif-2Α-Dependent Manner, Mengxia Qu, Yang Long, Yuqin Wang, Nan Yin, Xiaotian Zhang

Faculty, Staff and Student Publications

Autotaxin (ATX), the key enzyme that generates lysophosphatidic acid (LPA) from lysophosphatidylcholine (LPC), is involved in tumorigenesis through the ATX-LPA axis and is regarded as a valuable target in tumor therapy. Hypoxia is a major feature of solid tumors and contributes to tumor development with striking alterations in the gene expression profile. Here, we show that hypoxia induces ATX expression in a hypoxia-inducible factor (HIF) 2α-dependent fashion in human colon cancer SW480 cells. HIF-2α is directly bound to specific hypoxia response elements (HREs) in the ATX promoter. Under hypoxic conditions, knockout or inhibition of ATX suppressed the migration of SW480 …

Risk Of Early Death In Adolescents And Young Adults With Cancer: A Population-Based Study, Amy M Berkman, Clark R Andersen, Michelle A T Hildebrandt, J A Livingston, Adam L Green, Susan K Peterson, Kimberly A Miller, David R Freyer, Michael E Roth

Faculty, Staff and Student Publications

BACKGROUND: Advancements in treatment and supportive care have led to improved survival for adolescents and young adults (AYAs) with cancer; however, a subset of those diagnosed remain at risk for early death (within 2 months of diagnosis). Factors that place AYAs at increased risk of early death have not been well studied.

METHODS: The Surveillance, Epidemiology, and End Results registry was used to assess risk of early death in AYAs with hematologic malignancies, central nervous system tumors, and solid tumors. Associations between age at diagnosis, sex, race, ethnicity, socioeconomic status, insurance status, rurality, and early death were assessed.

RESULTS: A …

Upper Age Limits For Us Male Human Papillomavirus Vaccination For Oropharyngeal Cancer Prevention: A Microsimulation-Based Modeling Study, Rebecca Landy, Gregory Haber, Barry I Graubard, Nicole G Campos, Stephen Sy, Emily A Burger, Hormuzd A Katki, Maura L Gillison, Anil K Chaturvedi

Faculty, Staff and Student Publications

BACKGROUND: Human papillomavirus (HVP)-positive oropharyngeal cancer is the most common HPV-associated cancer in the United States. The age at acquisition of oral HPV infections that cause oropharyngeal cancer (causal infections) is unknown; consequently, the benefit of vaccination of US men aged 27-45 years remains uncertain.

METHODS: We developed a microsimulation-based, individual-level, state-transition model of oral HPV16 and HPV16-positive oropharyngeal cancer among heterosexual US men aged 15-84 years, calibrated to population-level data. We estimated the benefit of vaccination of men aged 27-45 years for prevention of oropharyngeal cancer, accounting for direct- and indirect effects (ie, herd effects) of male and female …

Molecular Mechanisms Of Ferroptosis And Updates Of Ferroptosis Studies In Cancers And Leukemia, Hiroki Akiyama, Bing Z Carter, Michael Andreeff, Jo Ishizawa

Faculty, Staff and Student Publications

Ferroptosis is a mode of cell death regulated by iron-dependent lipid peroxidation. Growing evidence suggests ferroptosis induction as a novel anti-cancer modality that could potentially overcome therapy resistance in cancers. The molecular mechanisms involved in the regulation of ferroptosis are complex and highly dependent on context. Therefore, a comprehensive understanding of its execution and protection machinery in each tumor type is necessary for the implementation of this unique cell death mode to target individual cancers. Since most of the current evidence for ferroptosis regulation mechanisms is based on solid cancer studies, the knowledge of ferroptosis with regard to leukemia is …

Structural Foundations Of Potassium Selectivity In Channelrhodopsins, Changsoo Chang

Faculty, Staff and Student Publications

Mitochondria are critical to cellular and organismal health. To prevent damage, mitochondria have evolved protein quality control machines to survey and maintain the mitochondrial proteome. SKD3, also known as CLPB, is a ring-forming, ATP-fueled protein disaggregase essential for preserving mitochondrial integrity and structure. SKD3 deficiency causes 3-methylglutaconic aciduria type VII (MGCA7) and early death in infants, while mutations in the ATPase domain impair protein disaggregation with the observed loss-of-function correlating with disease severity. How mutations in the non-catalytic N-domain cause disease is unknown. Here, we show that the disease-associated N-domain mutation, Y272C, forms an intramolecular disulfide bond with Cys267 and …

Quantitative Proteomic Screening Uncovers Candidate Diagnostic And Monitoring Serum Biomarkers Of Ankylosing Spondylitis, Reyna Chavez, Kamala Vanarsa, Chandra Mohan, John Reveille

Faculty, Staff and Student Publications

BACKGROUND: We sought to discover serum biomarkers of ankylosing spondylitis (AS) for diagnosis and monitoring disease activity.

METHODS: We studied biologic-treatment-naïve AS and healthy control (HC) patients' sera. Eighty samples matched by age, gender, and race (1:1:1 ratio) for AS patients with active disease, inactive disease, and HC were analyzed with SOMAscan™, an aptamer-based discovery platform. T-tests tests were performed for high/low-disease activity AS patients versus HCs (diagnosis) and high versus low disease activity (Monitoring) in a 2:1 and 1:1 ratio, respectively, to identify differentially expressed proteins (DEPs). We used the Cytoscape Molecular Complex Detection (MCODE) plugin to find clusters …

Improving Diabetes Management For School Health Workers Using A Live, Virtual Training Course, Bonnie Mccann-Crosby, Lisa Setchfield, Yvonne Barham, Dawn Espinosa, Jennifer Bailey, Beth Pali, Amanda Garey, Sarah Lyons, Rona Sonabend

Journal of Nursing & Interprofessional Leadership in Quality & Safety

Background: Type 1 diabetes is one of the most common chronic diseases in childhood. Optimal school nurse and staff education is critical to creating a safe learning environment and influencing long term outcomes of students with type 1 diabetes. The purpose of this project was to develop a virtual continuing education diabetes management course for school health workers to improve their knowledge of diabetes management and evaluate the effectiveness of the learning platform.

Methods: A total of 199 participants completed the live, virtual continuing education course. Participants completed a pre and post-test to assess improvements in the key learning objectives …

Transient Premature Ovarian Insufficiency Post-Covid-19 Infection., Colleen N. Gorman, Tori E. Abdalla, Yasmina Sultan, Spencer A. Grabois, Ellen G. Wood

HPD Articles

Anti-ovarian antibodies (AOAs) have been linked to autoimmune premature ovarian insufficiency (POI). This report details a case in which a patient experienced transient POI after a COVID-19 infection and tested positive for AOA. After treatment with oral contraceptives and subsequent high-dose oral corticosteroids, the patient underwent fertility treatment with in vitro fertilization (IVF). A total of 23 oocytes were retrieved. Two euploid blastocysts and three untested blastocysts were successfully created. This report hypothesizes the connection between autoimmune POI, AOA, and COVID-19. Conflicting data have been reported linking COVID-19 and ovarian injury. However, it is suggested that COVID-19 transiently impacts the …

2023-04-10_Michiganoverview_Covidbedppe, Michigan Department Of Health & Human Services

Michigan COVID-19 Hospital Data

Contains the tables Statewide Hospital Capacity Report for COVID-19, COVID-19 Metrics, Statewide Hospital Available PPE Tracking for COVID-19, as well as the tables PPE Days On Hand and Patient Census.

Nashville Dolphins Volunteer Training Development, Caitlin Barnickel

OTD Capstone Projects

This doctoral capstone project was completed through a partnership with Nashville Dolphins. The Nashville Dolphins offer free swim lessons and swim team programs for children and adults with disabilities. They serve over 300 participants each season and the programs are entirely run by volunteers. The focus of this project was to identify current needs and develop more volunteer training resources. These resources incorporate; sensory processing strategies, behavior management techniques, visual schedule information, communication strategies, description of specific water properties, skill development tips, equipment recommendations, and general tips for teaching children with disabilities. Volunteers were provided with a resource guide and …

Building Resilience Through Pregnancy Utilizing Tools From Occupational Therapy And Yoga, Rachel Brown

OTD Capstone Projects

This doctoral capstone project was completed through a partnership with Nurture Nashville, a local yoga and movement studio focused on promoting wellness and connection for pregnant and postpartum people throughout their journey in motherhood. The primary focus of this project was developing resources to promote resilience during the perinatal period by utilizing tools from both occupational therapy and yoga practice. Outcomes included the synthesis of research into a resilience toolkit, the creation and implementation of a 5-week yoga and sharing circle curriculum, and the re-establishment of a community-centered studio event titled “Meet the Doulas.”

The Impact Of Social Emotional Learning In Pre-Kindergarten Classrooms, Kendall C. Jackson

OTD Capstone Projects

The Impact of Social emotional learning supports in early childhood education. First Steps Inc. is both an outpatient clinic and childcare facility for children with and without special needs and medical conditions. This capstone project attempts to bridge the gap between the therapy team and education team by creating therapeutic supports to help implement and teach social emotional learning in the classroom.

Nashville Dolphins Program Development, Laura Graves

OTD Capstone Projects

This doctoral capstone project was completed through a partnership with Nashville Dolphins. The Nashville Dolphins offer free swim lessons and swim team programs for children and adults with disabilities. The organization serves over 300 swimmers each season and the programs are entirely run by volunteers. The focus of this project was to develop programming resources to enhance the services offered by the Nashville Dolphins. Deliverables provided to the organization include an 8-week swim curriculum, color level guides, stroke checklists, and an equipment activity guide.

Spatial Characteristics Of Reactive Stepping Among People Living With Chronic Incomplete Spinal Cord Injury, Matthew G Heffernan, Jae Woung Lee, Katherine Chan, Janelle Unger, Susan Marzolini, Timothy N Welsh, Kei Masani, Kristin E Musselman

Physical Therapy Publications

Objective: Compare the spatial characteristics of reactive stepping between individuals with chronic motor incomplete spinal cord injuries (iSCI) and able-bodied (AB) individuals.

Design: Cross sectional.

Setting: Lyndhurst Centre.

Participants: Twelve individuals with iSCI (3 males, 53.6 ± 15.2 years old) and 11 age- and sex-matched AB individuals (3 males, 54.8 ± 14.0 years old).

Interventions: The Lean-and-Release test was used to elicit reactive stepping. A horizontal cable, attached at waist height, was released when 8-12% body weight was supported in a forward lean position. Participants underwent up to 10 Lean-and-Release trials in a session. Kinematic and …

Week 13: Promoting Occupational Balance During Trauma Healing Journey Post-Program Participation, Rachel Hicks

OTD Capstone Projects

This doctoral capstone project was completed in a partnership with REBOOT Recovery, a non-profit organization dedicated to promoting trauma healing through participation in faith-based, peer-led courses. The purpose of this project was to provide program participants with additional content to promote improved occupational performance as they continue into the next phase of the healing journey after completing the original 12-week courses. The project was given the title “Week 13” within the organization to illustrate its role in guiding members’ next steps in trauma healing. Content areas included rebuilding healthy routines, reclaiming restful sleep, conflict resolution in personal relationships, education on …

Nashville Dolphins Program Development, Laura Graves

OTD Capstone Projects

This doctoral capstone project was completed through a partnership with Nashville Dolphins. The Nashville Dolphins offer free swim lessons and swim team programs for children and adults with disabilities. The organization serves over 300 swimmers each season and the programs are entirely run by volunteers. The focus of this project was to develop programming resources to enhance the services offered by the Nashville Dolphins. Deliverables provided to the organization include an 8-week swim curriculum, color level guides, stroke checklists, and an equipment activity guide.

Ada News - 04/10/2023, American Dental Association, Publishing Division

ADA News

Established in 1970 as the official newspaper of the American Dental Association, the ADA News serves practicing dentists and others allied to the dental profession in the U.S. and internationally. It is the No. 1 source of news and information about the many benefits and services the ADA delivers to members daily as well as timely information on scientific, social, political and economic developments affecting dentistry and health care.

Last Winter’S Tripledemic: A Multi-Factorial Syndrome Of Respiratory Illness, Kara Hom

URGENT Matters

No abstract provided.

Electrodermal Activity In Response To Stress Reduction: Virtual Reality Experience In Hydrotherapy And Non-Hydrotherapy Environments, Nthabeleng Macdonald

Undergraduate University Honors Capstones

Hydrotherapy, a holistic alternative medicine, uses methods like water therapy, cold water immersion, and balneotherapy to treat physical and mental illnesses while inducing tranquility (Bahadorfar, 2014). In this study, the water therapy approach was used, but with a unique twist. Rather than traditional hydrotherapy involving direct contact with water, participants were fully immersed in a virtual reality water environment. This study investigated whether immersing individuals with psychological stress in an underwater environment via virtual reality (VR) reduces their stress levels as compared to a non-hydrotherapy virtual city environment. The effects of underwater scenery versus non-hydrotherapy city scenery individuals experiencing stress …

Covid-19 Vaccination: A Study Of College Students’ Perceptions Regarding Inoculation Post-Covid Infection, Analisa Harter, Wilma Matti

Honors College Theses

Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), known as Covid-19, was first identified in China and proclaimed a pandemic in March 2020 by the World Health Organization (WHO). Covid-19 is a virus transmitted via respiratory droplets and becomes airborne when a person who carries the virus coughs, sneezes, or breathes out droplets, and the virus enters a susceptible host. Since the pandemic outbreak, three vaccines have been approved by the Food and Drug Administration (FDA). Despite FDA approval, many Americans are hesitant to receive Covid-19 vaccinations. The perceived severity of SARS-CoV-2 and the perceived safety of the vaccine appear to …

Uropathogenic Escherichia Coli Infection-Induced Epithelial Trained Immunity Impacts Urinary Tract Disease Outcome, Seongmi K Russell, Jessica K Harrison, Benjamin S Olson, Hyung Joo Lee, Valerie P O'Brien, Xiaoyun Xing, Lu Yu, Elisha D O Roberson, Rajdeep Bomjan, Changxu Fan, Marina Sha, Marco Colonna, Ting Wang, Thomas J Hannan, Scott J Hultgren, Et Al.

2020-Current year OA Pubs

Previous urinary tract infections (UTIs) can predispose one to future infections; however, the underlying mechanisms affecting recurrence are poorly understood. We previously found that UTIs in mice cause differential bladder epithelial (urothelial) remodelling, depending on disease outcome, that impacts susceptibility to recurrent UTI. Here we compared urothelial stem cell (USC) lines isolated from mice with a history of either resolved or chronic uropathogenic Escherichia coli (UPEC) infection, elucidating evidence of molecular imprinting that involved epigenetic changes, including differences in chromatin accessibility, DNA methylation and histone modification. Epigenetic marks in USCs from chronically infected mice enhanced caspase-1-mediated cell death upon UPEC …

Il-15 Enhances Hiv-1 Infection By Promoting Survival And Proliferation Of Ccr5+Cd4+ T Cells, Yuhao Li, Hongbo Gao, Kolin M. Clark, Liang Shan

2020-Current year OA Pubs

HIV-1 usually utilizes CCR5 as its coreceptor and rarely switches to a CXCR4-tropic virus until the late stage of infection. CCR5+CD4+ T cells are the major virus-producing cells in viremic individuals as well as SIV-infected nonhuman primates. The differentiation of CCR5+CD4+ T cells is associated with the availability of IL-15, which increases during acute HIV-1 infection. Here, we report that CCR5 was expressed by CD4+ T cells exhibiting effector or effector memory phenotypes with high expression levels of the IL-2/IL-15 receptor common β and γ chains. IL-15, but not IL-7, improved the survival of CCR5+CD4+ T cells, drove their expansion, …

Increased Clonal Hematopoiesis Involving Dna Damage Response Genes In Patients Undergoing Lung Transplantation, Laneshia K Tague, Karolyn A. Oetjen, Anirudh Mahadev, Matthew J. Walter, Hephzibah Anthony, Daniel Kreisel, Daniel C. Link, Andrew E Gelman

2020-Current year OA Pubs

BACKGROUNDCellular stressors influence the development of clonal hematopoiesis (CH). We hypothesized that environmental, inflammatory, and genotoxic stresses drive the emergence of CH in lung transplant recipients. METHODSWe performed a cross-sectional cohort study of 85 lung transplant recipients to characterize CH prevalence. We evaluated somatic variants using duplex error-corrected sequencing and germline variants using whole exome sequencing. We evaluated CH frequency and burden using χ2 and Poisson regression, and we evaluated associations with clinical and demographic variables and clinical outcomes using χ2, logistic regression, and Cox regression. RESULTSCH in DNA damage response (DDR) genes TP53, PPM1D, and ATM was increased in …

A Ferritin Nanoparticle-Based Zika Virus Vaccine Candidate Induces Robust Humoral And Cellular Immune Responses And Protects Mice From Lethal Virus Challenge, Aryamav Pattnaik, Bikash R. Sahoo, Lucas R. Struble, Gloria E. O. Borgstahl, You Zhou, Rodrigo Franco, Raul G. Barletta, Fernando A. Osorio, Thomas M. Petro, Asit K. Pattnaik

School of Veterinary and Biomedical Sciences: Faculty Publications

The severe consequences of the Zika virus (ZIKV) infections resulting in congenital Zika syndrome in infants and the autoimmune Guillain–Barre syndrome in adults warrant the development of safe and efficacious vaccines and therapeutics. Currently, there are no approved treatment options for ZIKV infection. Herein, we describe the development of a bacterial ferritin-based nanoparticle vaccine candidate for ZIKV. The viral envelope (E) protein domain III (DIII) was fused in-frame at the amino-terminus of ferritin. The resulting nanoparticle displaying the DIII was examined for its ability to induce immune responses and protect vaccinated animals upon lethal virus challenge. Our results show that …

Prevalence And Beliefs Associated With Vaccine Hesitancy Among Muslim-Americans, Marina Ali

Health and Kinesiology Theses

Background. Vaccine hesitancy hinders the eradication of preventable illnesses. Furthermore, there are gaps in public health research on vaccine hesitancy among Muslims in heterogenous societies.

Objective. This study aimed to determine whether socioeconomic demographics, political beliefs and trust in public institutions were associated with vaccine hesitancy beliefs. Additionally, two models were applied to determine which factors had the strongest influence in rejecting vaccines.

Method. Participants were recruited through Facebook group posts. Seventy-three responses were received. Sixty-three responses met the inclusion criteria and were included in the final analysis. Participants rated their opinions on political beliefs, religious practices, trust in institutions …

Commitment To Collaboration: Development Of A School Nursing Collaboration Instrument, Jodi S. Bullard

Nursing Theses and Dissertations

Registered nurses (RNs) have an ethical responsibility to collaborate, thereby promoting the scope of nursing practice and working to positively influence health outcomes. This responsibility encompasses RNs employed as school nurses (SNs). Examples of SN collaboration with campus, school district, and community health partners exist. However, SNs’ commitment to collaboration in these relationships has not been examined.

Chapter 2, “COVID-19: Planning and Postpandemic Partnerships,” explores surges in school nursing partnerships that occur during healthcare emergencies. The significance of partnering with SNs in the school, school district, and community setting is discussed. The need to develop school nursing partnerships that extend …

Scar Formation In The Presence Of Mitomycin C And The Anti-Fibrotic Antibody In A Rabbit Model Of Glaucoma Microsurgery: A Pilot Study, Aakriti Garg Shukla, Tatyana Milman, Jolanta Fertala, Andrzej Steplewski, Andrzej Fertala

Wills Eye Hospital Papers

Purpose: This study aimed to evaluate the utility of a rationally engineered antibody that directly blocks collagen fibrillogenesis to reduce scar tissue formation associated with subconjunctival glaucoma surgery.

Material and methods: Fourteen eyes of 7 adult rabbits underwent glaucoma filtering surgery using XEN 45 Gel Stent. The rabbits’ eyes were divided randomly into three treatment groups: (i) treated with the antibody, (ii) treated with mitomycin C, and (iii) treated with the antibody and mitomycin C. Following surgeries, the intraocular pressure and bleb appearance were evaluated in vivo. The rabbits were sacrificed 8 weeks after the surgery, and their eyes were …

Life Skills Guide For Supported Recovery For Individuals With Mental Health Conditions & Their Support Systems, Natalie Metzger

OTD Capstone Projects

The National Alliance on Mental Illness of Davidson County provides educational courses, support groups, and advocacy for individuals with mental health conditions and their support systems. This semester long project included advocacy for occupational therapy’s role in the mental health community, involvement in legislative events, and creation of a comprehensive recovery life skills guide for individuals who have been discharged from psychiatric hospitals. Specifically, this guide assisted them in directed recovery within the home and increased their participation in foundational skills.

Program Development Through Therapeutic Camps, Bayleigh B. Powers

OTD Capstone Projects

This doctoral capstone project was completed through partnership with Wonderlight Therapy, a mobile pediatric therapy clinic offering speech and occupational therapy in Nashville, TN. The focus of this project was to develop and expand sustainable & inclusive community programming to serve the greater Nashville area. Throughout this project, 9 new & inclusive community programs (camps) were created, planned, and some implemented to children Wonderlight serves. Additionally, the creation of a sponsorship/scholarship program was implemented, offering local businesses the chance to support inclusive camps in their community.

Comprehensive Review Of Acute Pancreatitis Pain Syndrome, Jacob Beiriger, Adnan Khan, Brian Yan, Heather Ross, Makala Wang, Michael Carducci, Natali Salinas, Salil Chowdhury, Ryan Erwin, Sarah Chen, Alexis Gerber

Jefferson Hospital Staff Papers and Presentations

Pancreatitis is a condition that causes inflammation in the pancreas, an organ located behind the stomach. This condition often presents as neuropathic, inflammatory, and/or visceral pain. Acute pancreatitis is typically characterized by sudden and severe abdominal pain, often in the upper right part of the abdomen. The pain from pancreatitis can be caused by different mechanisms, such as abnormal activation of pancreatic zymogens or NF-κB mediated inflammation in the pancreas. The treatment of pancreatitis depends on its type, severity, and underlying cause. Hospitalization and medications are typically necessary, while in others, surgery may be required. Proper management of pancreatitis is …