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Full-Text Articles in Medicine and Health Sciences

Therapeutic Potential Of Bulgarian Propolis Against Antibiotic-Resistant Salmonella Species, Manar M. Farouk, Amal El-Molla, Fayez A. Salib, Yousef A. Soliman, Mohamed Shaalan Mar 2022

Therapeutic Potential Of Bulgarian Propolis Against Antibiotic-Resistant Salmonella Species, Manar M. Farouk, Amal El-Molla, Fayez A. Salib, Yousef A. Soliman, Mohamed Shaalan

The Thai Journal of Veterinary Medicine

The emergence of multidrug-resistant (MDR) Salmonella spp. has increased the need for investigation of antimicrobial alternatives. Ethanolic extract of propolis (EEP) has been identified to have wide-ranging medical applications related to its antimicrobial activity. The current study aimed to identify the major chemical components of EEP with antibacterial effects, evaluate the antibacterial efficacy of EEP against MDR Salmonella spp. isolates, and further examine the therapeutic efficacy of EEP against MDR S. Enteritidis infected mouse model. Bulgarian propolis EEP was prepared by using ethanol based extraction method. The chemical composition of EEP was characterized using the gas chromatography-mass spectroscopy (GC-MS). The …


The Effect Of Recombinant Chimeric Swine Pkr-Apaf-1 Proteins On Viral-Load Reduction Of Prrs And Ped Alpha-Coronavirus Infected Cell Lines, Phong Vu Anh Tuan Vo, Thanaporn Lertsakchai, Sukeesom Jiramahasuwan, Achara Tawatsin, Athipoo Nuntaprasert Mar 2022

The Effect Of Recombinant Chimeric Swine Pkr-Apaf-1 Proteins On Viral-Load Reduction Of Prrs And Ped Alpha-Coronavirus Infected Cell Lines, Phong Vu Anh Tuan Vo, Thanaporn Lertsakchai, Sukeesom Jiramahasuwan, Achara Tawatsin, Athipoo Nuntaprasert

The Thai Journal of Veterinary Medicine

The PRRS virus and PED alpha-coronavirus are the two main pig pathogens in the world. The current strategies to control these diseases are inadequate. The recombinant chimeric swine PKR-Apaf-1 protein (rcPAP) consists of the Human Immunodeficiency Virus Trans-activator transcription (HIV-TAT) domain, the dsRNA-binding domain of porcine Protein kinase R (PKR) gene and the caspase recruitment domain (CARD) domain of porcine Apoptotic Protease-Activating Factor-1 (Apaf-1) gene. In general, the rcPAP can detect viral dsRNA in virally infected cells and induces apoptosis and kills the virus-infected cells. In this study, the reducing viral-load efficacy of rcPAP (produced from bacteria) against PRRS virrus …


Shock Status And Anti-Shock Infusion Therapy In Canine Acute Spontaneous Babesiosis, Oksana Dubova, Lyudmila Kovalova, Anatolyi Dubovyi, Pavel Kovalov, Inna Chala Mar 2022

Shock Status And Anti-Shock Infusion Therapy In Canine Acute Spontaneous Babesiosis, Oksana Dubova, Lyudmila Kovalova, Anatolyi Dubovyi, Pavel Kovalov, Inna Chala

The Thai Journal of Veterinary Medicine

Canine babesiosis is a serious disease that is accompanied by the development of shock. The aim of the work was to establish shock, assess its degree, as well as to conduct a clinical trial of anti-shock infusion therapy. 100 dogs of different breeds, weighing 20–40 kg, aged 2–4 years, were involved. The studies used oscillometry (blood pressure, pulse rate), assessment of vascular-platelet hemostasis (platelet count, spontaneous aggregation ability), coagulogram (prothromine and activated partial thromboplastin time, fibrinogen), enzyme immunoassay (fibrin degradation products and D-dimer), biochemical (soluble fibrin-monomer complexes) and hemodynamic (circulating blood volume, hematocrit) methods of investigation. In dogs with acute …


The Relationship Of Abortion, Dog Management And Neospora Caninum In Dairy Herds In Taiwan, Hsu-Hsun Lee, Chen-Chih Chen, Yi-Lun Tsai, Shyh-Shyan Liu, Po-An Yueh, Huan-Yu Hsu, Ai-Mei Chang Mar 2022

The Relationship Of Abortion, Dog Management And Neospora Caninum In Dairy Herds In Taiwan, Hsu-Hsun Lee, Chen-Chih Chen, Yi-Lun Tsai, Shyh-Shyan Liu, Po-An Yueh, Huan-Yu Hsu, Ai-Mei Chang

The Thai Journal of Veterinary Medicine

Neospora caninum (N. caninum) has caused an abortion storm in dairy herds for two decades in Taiwan. There is no effective medicine for N. caninum infection for livestock, therefore, prevention by farm management is very important. The risk factors for N. caninum infection in Taiwan is still unclear. Dogs are the common definitive hosts of N. caninum on farms. This study aims to estimate the sero-prevalence and detection of N. caninum DNA in 361 pregnant dairy cows and establish an effective strategy to prevent abortion caused by N. caninum in dairy farms in Taiwan by logistic regression. The sero-prevalence of …


Trace Elements, Oxidative Stress Biomarkers And Hemato-Biochemical Indices Of Goat Kids Affected With Nutritional Dependent Locomotor Disturbances, Issam Eljalii, Taha Fouda, Wael El-Deeb, Abdulaziz Almujalli, Sabry M. El-Bahr Mar 2022

Trace Elements, Oxidative Stress Biomarkers And Hemato-Biochemical Indices Of Goat Kids Affected With Nutritional Dependent Locomotor Disturbances, Issam Eljalii, Taha Fouda, Wael El-Deeb, Abdulaziz Almujalli, Sabry M. El-Bahr

The Thai Journal of Veterinary Medicine

This study aimed to investigate the biomedical significant of trace elements, oxidative stress and hemato-biochemical indices in the diagnosis of locomotor disturbances of affected goat kids. Blood samples were collected from 22 goat kids affected with locomotor disturbances. An equal number of healthy goat kids was used as a control. Clinical examination showed anemia and an incoordination in affected animals compared to the control. Malondialdehyde (MDA), creatine kinase (CK) and aspartate aminotransferase (AST) values were significantly higher in affected animals than in the control, while superoxide dismutase (SOD) and reduced glutathione (GSH) values were significantly lower. Concentrations of copper (Cu), …


Biomechanical Analysis Of Atlantoaxial Dorsal Fixation Using Finite Element Models, Beomju Bae, Dongwook Kim, Hyejong Oh, Gonhyung Kim Mar 2022

Biomechanical Analysis Of Atlantoaxial Dorsal Fixation Using Finite Element Models, Beomju Bae, Dongwook Kim, Hyejong Oh, Gonhyung Kim

The Thai Journal of Veterinary Medicine

Atlantoaxial instability can cause spinal cord compression with clinical signs ranging from cervical pain to tetraplegia and death. Although a variety of dorsal fixation techniques have been described, some of them have been related to the fracture of the dorsal arch of the atlas, leading to surgical failure. Under the hypothesis that the shape of the dorsal arch of the atlas and types of implants might affect these bone fractures, the objective of this study was to analyze bone stresses through simulations of the dorsal fixation using finite element models. Arbitrary tension forces were given to implants for simulations of …


A Dorsal Sling Technique In A Dermoid Sinus Dog With Nuchal Ligament Excision, Nithida Boonwittaya, Pakthorn Lewchalermwong, Kanokwan Keadwut Mar 2022

A Dorsal Sling Technique In A Dermoid Sinus Dog With Nuchal Ligament Excision, Nithida Boonwittaya, Pakthorn Lewchalermwong, Kanokwan Keadwut

The Thai Journal of Veterinary Medicine

A dorsal sling technique for correction of nuchal ligament excision in a dermoid sinus canine case is presented in this report. At surgery, there were multiple tubes of the dermoid sinus tracts of different types. The nuchal sinus was treated using a segmental resection at the area of involvement and a dorsal sling technique was performed. The dog recovered uneventfully and regained normal daily activities within a short time after surgery. The technique was accomplished as evaluated by non-pain normal motion of the cervical region and no complications were found during the follow-up period. This is the first report on …


The Validation Of Haemagglutinin Inhibition Test For The Detection Of Antibodies Against Canine Influenza Virus In A Guinea Pig Serum Model, Ratanaporn Tangwangvivat, Supassama Chaiyawong, Nutthawan Nonthabenjawan, Kamonpan Charoenkul, Taveesak Janethanakij, Duangduen Prakairungnamthip, Wittawat Wechtaisong, Supanat Boonyapisitsopa, Napawan Bunpapong, Alongkorn Amonsin Mar 2022

The Validation Of Haemagglutinin Inhibition Test For The Detection Of Antibodies Against Canine Influenza Virus In A Guinea Pig Serum Model, Ratanaporn Tangwangvivat, Supassama Chaiyawong, Nutthawan Nonthabenjawan, Kamonpan Charoenkul, Taveesak Janethanakij, Duangduen Prakairungnamthip, Wittawat Wechtaisong, Supanat Boonyapisitsopa, Napawan Bunpapong, Alongkorn Amonsin

The Thai Journal of Veterinary Medicine

No abstract provided.


Evaluation Of Changes In Some Enzymatic And Non-Enzymatic Antioxidants In Cats With Feline Panleukopenia, Afshin Behzadian, Ameneh Khoshvaghti Mar 2022

Evaluation Of Changes In Some Enzymatic And Non-Enzymatic Antioxidants In Cats With Feline Panleukopenia, Afshin Behzadian, Ameneh Khoshvaghti

The Thai Journal of Veterinary Medicine

Feline panleukopenia is a serious contagious viral disease of cats that has been identified as the causative agent of death in 25% of kittens sent for pathological examination. Antioxidant systems are one of the very important defense systems of body. The aim of the current study was to evaluate changes in serum levels of Vitamin D, glutathione peroxidase and superoxide dismutase in cats infected with panleukopenia virus. Ten healthy male cats and ten male cats with clinical and laboratory symptoms of panleukopenia with a mean age of 6±2 months were selected. The Glutathione peroxidase and superoxide dismutase were measured by …


Indications For Canine Fecal Microbiota Transplantation, Kerong Li, Xiaoxiao Zhou, Zhijun Zhong, Haifeng Liu, Min Li, Guangneng Peng, Ziyao Zhou Mar 2022

Indications For Canine Fecal Microbiota Transplantation, Kerong Li, Xiaoxiao Zhou, Zhijun Zhong, Haifeng Liu, Min Li, Guangneng Peng, Ziyao Zhou

The Thai Journal of Veterinary Medicine

Fecal microbiota transplantation (FMT) is a novel method to treat intestinal and extraintestinal diseases by transplanting the microbiota in the feces of healthy animals into the gastrointestinal tracts of patients and reconstructing intestinal flora in the patients' intestine. In this article, we reviewed the recent studies of canine FMT focusing on its indications. FMT was proved effective to treat canine Clostridioides difficile infection (CDI), inflammatory bowel disease (IBD), canine parvovirus (CPV) diarrhea and acute diarrhea; upregulate the intestinal microbiota in dogs with acute hemorrhagic diarrhea syndrome (AHDS); and may have benefit for preventing canine post-weaning diarrhea. However, the specific FMT …


Comparison Of An Intra-Articular Suture Technique And A Lateral Suture Technique In Toy Breed Dogs With Cranial Cruciate Ligament Rupture, Sirun Tuek-Um, Chaiyakorn Thitiyanaporn, Phitchaya Phongwirat, Naris Thengchaisri Mar 2022

Comparison Of An Intra-Articular Suture Technique And A Lateral Suture Technique In Toy Breed Dogs With Cranial Cruciate Ligament Rupture, Sirun Tuek-Um, Chaiyakorn Thitiyanaporn, Phitchaya Phongwirat, Naris Thengchaisri

The Thai Journal of Veterinary Medicine

Cranial cruciate ligament rupture (CCLR) can result in inflammation, pain, lameness and osteoarthritis. Because there is no isometric point for surgical stabilization for CCLR, the anatomical replacement of the ruptured cranial cruciate ligament with synthetic material may provide better outcomes. This retrospective study compared short-term post-operative outcomes between an intra-articular suture technique and a lateral suture technique for treating CCLR in toy breed dogs. Twenty dogs with unilateral CCLR were divided into two groups. The lateral suture technique was a lateral circumfabellar tibial suture with monofilament nylon and the intra-articular suture was performed by passing a suture through the femoral …


Complete Genome Sequence Of A Novel Recombinant Muscovy Duck Parvovirus Isolated In The Heilongjiang Province Of China, Jing Yang, Ming Li, Tianfei Yu Mar 2022

Complete Genome Sequence Of A Novel Recombinant Muscovy Duck Parvovirus Isolated In The Heilongjiang Province Of China, Jing Yang, Ming Li, Tianfei Yu

The Thai Journal of Veterinary Medicine

Muscovy duck parvovirus (MDPV) is fatal for young ducklings of less than 3 weeks of age and has caused severe economic loss in duck production in many countries and regions. We report here on a complete genome sequence of a recombinant MDPV strain YL08 isolated from an ill Muscovy duck in 2008 at Qiqihar, Heilongjiang province, China. This genome, MDPV YL08, was 5061 nucleotides long and retained the two ORFs, which are typical in the Parvovirinae family. The genome of YL08 strain has a putative recombination breakpoint on its VP1 gene. This study has helped our understanding of the genetic …


Phylogenetic Positioning Of Listeria Ivanovii Identified In Aborted Sheep In Kars Region (Turkey), Dogan Akca, Fatih Buyuk, Elif Celik, Aliye Gulmez Saglam, Salih Otlu, Serpil Dag, Ozgur Celebi, Mustafa Reha Coskun, Eray Buyuk, Emin Karakurt, Mitat Sahin Mar 2022

Phylogenetic Positioning Of Listeria Ivanovii Identified In Aborted Sheep In Kars Region (Turkey), Dogan Akca, Fatih Buyuk, Elif Celik, Aliye Gulmez Saglam, Salih Otlu, Serpil Dag, Ozgur Celebi, Mustafa Reha Coskun, Eray Buyuk, Emin Karakurt, Mitat Sahin

The Thai Journal of Veterinary Medicine

This study aimed to investigate Listeria species in various samples taken from aborted sheep in Kars province and to determine the position of emerged strains in the phylogenetic tree. Milk (n= 229) and vaginal swab (n= 263) samples from aborted sheep and abomasum contents (n= 46) of aborted sheep fetuses were investigated by the culture and Polymerase Chain Reaction (PCR) methods. For the phylogenetic analysis of isolates, the 16S rRNA gene region was sequenced. In the culture, the Listeria agent was not isolated from any of the vaginal swabs or milk samples. Among the 46 fetal abomasum contents, a Listeria …


Natural Mycoplasma Bovis Infection Associated With Epithelioid Granulomatous Caseonecrotic Bronchiolitis At A Progressed Disease Stage In Calves, Mathurot Suwanruengsri, Ryoko Uemura, Uda Zahli Izzati, Takuya Kanda, Naoyuki Fuke, Masahiro Yasuda, Takuya Hirai, Ryoji Yamaguchi Mar 2022

Natural Mycoplasma Bovis Infection Associated With Epithelioid Granulomatous Caseonecrotic Bronchiolitis At A Progressed Disease Stage In Calves, Mathurot Suwanruengsri, Ryoko Uemura, Uda Zahli Izzati, Takuya Kanda, Naoyuki Fuke, Masahiro Yasuda, Takuya Hirai, Ryoji Yamaguchi

The Thai Journal of Veterinary Medicine

Mycoplasma bovis (M. bovis) is a pathogen that especially effects the respiratory system of cattle. We focused on the histopathological changes in the lungs caused by M. bovis because the lesions in the bronchi or bronchioles and alveoli are quite different in natural infections. Thirty-five lung samples from 2-to 12-month-old Japanese Black calves with respiratory symptoms were collected and examined for bacteria presence and pathological changes using immunohistochemistry (IHC). Using the loop-mediated isothermal amplification method, 18 of the 35 samples were found to be positive for M. bovis, which was isolated and identified. In 7 of the samples, only M. …


Surveillance Of Zoonotic Brugia Pahangi In Monastery Cats, Samphran District, Nakhon Pathom, Thailand, Thanakorn Rawangchue, Nuttasiri Sripirom, Sivapong Sungpradit Mar 2022

Surveillance Of Zoonotic Brugia Pahangi In Monastery Cats, Samphran District, Nakhon Pathom, Thailand, Thanakorn Rawangchue, Nuttasiri Sripirom, Sivapong Sungpradit

The Thai Journal of Veterinary Medicine

The mosquito-borne filarial nematode parasites Brugia pahangi and B. malayi have been proposed as potential zoonotic pathogens, with dogs and cats being the main reservoir hosts. The monasteries where both stray and semi-domesticated animals live often serve as areas where humans are exposed to these reservoirs. In Thailand, the prevalence of B. pahangi, particularly in infected monastery cats, is underreported. In this study, we investigated B. pahangi prevalence in cats at 24 temples in the Samphran district, Nakhon Pathom province, central Thailand. In total, 196 blood samples were collected from the cats and examined using the hematocrit capillary tube technique. …


Clinical Characteristics, Antimicrobial Resistance And Treatment Outcomes Of Multidrug-Resistant Escherichia Coli Infection In Dogs And Cats At A Veterinary Teaching Hospital In Thailand, Jeerawat Soonthornsit, Sukanya Apiratwarrasakul, Nathita Phumthanakorn Mar 2022

Clinical Characteristics, Antimicrobial Resistance And Treatment Outcomes Of Multidrug-Resistant Escherichia Coli Infection In Dogs And Cats At A Veterinary Teaching Hospital In Thailand, Jeerawat Soonthornsit, Sukanya Apiratwarrasakul, Nathita Phumthanakorn

The Thai Journal of Veterinary Medicine

This retrospective study investigated the clinical characteristics of multidrug-resistant (MDR) Escherichia coli and its antimicrobial resistance phenotypes and analyzed the treatment outcomes of MDR E. coli infection in dogs and cats. The medical data of dogs and cats diagnosed in 2020 with E. coli infection at a veterinary teaching hospital were analyzed. Of 94 cases, the frequency of MDR E. coli (66%) infection was higher than that of non-MDR E. coli (34%). MDR E. coli was significantly more frequently detected in female dogs than non-MDR E. coli (P < 0.026). The most frequent MDR E. coli isolation sites were the urinary tract in dogs and skin wounds in cats. MDR E. coli isolates from dogs were highly resistant to ampicillin (96.1%), enrofloxacin (80.4%) and tetracycline (78.4%). Resistance to ampicillin (100%), enrofloxacin (90.9%), marbofloxacin (72.7%) and tetracycline (72.7%) occurred frequently in MDR E. coli isolates from cats. Low resistance to amikacin was detected in the MDR E. coli isolates from dogs and cats. The rates of clinical cure and non-clinical cure of the MDR E. coli and non-MDR E. coli cases were not significantly different. The duration of antimicrobial treatment for MDR E. coli was significantly longer in cats (12.6 ± 5.85 days) than in non-MDR E. coli cases (7 ± 0 days) (P < 0.048). Adjunctive therapy was prescribed more frequently in MDR E. coli (20%) than non-MDR E. coli cases (8.3%). The presence of MDR E. coli was high in this study. High resistance to commonly used antimicrobial drugs and treatment complications was observed in this study.


Naturally Occurring Peripheral T-Cell Lymphoma Not Otherwise Specified (Ptcl-Nos) With Systemic Dissemination In A Beagle, Ploen Nganthavee, Phuttipan Channgam, Tanit Kasantikul, Nlin Arya, Sirintra Sirivisoot, Tuddow Thaiwong, Zachary Millman, Rungrote Osathanon Mar 2022

Naturally Occurring Peripheral T-Cell Lymphoma Not Otherwise Specified (Ptcl-Nos) With Systemic Dissemination In A Beagle, Ploen Nganthavee, Phuttipan Channgam, Tanit Kasantikul, Nlin Arya, Sirintra Sirivisoot, Tuddow Thaiwong, Zachary Millman, Rungrote Osathanon

The Thai Journal of Veterinary Medicine

A 6-year-old intact female Beagle dog presented with acute vomiting. Abdominal ultrasound showed abdominal lymphadenopathy, ascites and a hyperechoic mass within the pancreas. Thoracic radiographs and echocardiography revealed cardiomegaly with pericardial effusion. The dog died following rapid deterioration and a subsequent necropsy revealed a multilobulated white-to-tan mass within the pancreas, attaching to the omentum, stomach and left adrenal gland. The heart was enlarged and infiltrated by similar coalescing off-white irregular masses. Histologically, the masses consisted of infiltrative sheets of large T lymphocytes replacing the normal architecture of the heart along with the lymph nodes, pancreas, stomach, omentum, left adrenal gland, …


Effects Of Atipamezole And Flumazenil On Stress-Related Hormonal And Metabolic Responses In Cats Anesthetized With Medetomidine, Midazolam, Ketamine And Isoflurane Undergoing Ovariohysterectomy And Castration, Hirokazu Kamohara, Toshiko Kamohara, Yoshiaki Hikasa Mar 2022

Effects Of Atipamezole And Flumazenil On Stress-Related Hormonal And Metabolic Responses In Cats Anesthetized With Medetomidine, Midazolam, Ketamine And Isoflurane Undergoing Ovariohysterectomy And Castration, Hirokazu Kamohara, Toshiko Kamohara, Yoshiaki Hikasa

The Thai Journal of Veterinary Medicine

This study examined the post-operative effects of atipamezole (Ati) and flumazenil (Flu) on stress-related neurohormonal and metabolic responses in isoflurane-anesthetized cats premedicated with medetomidine, midazolam and ketamine and undergoing ovariohysterectomy or castration. Client-owned mixed-breed cats (n = 108) were treated with 50-μg/kg medetomidine and 0.5-mg/kg midazolam, followed by 5-mg/kg ketamine (MMK) intramuscularly (IM) and maintained under isoflurane anesthesia. The cats were divided into nine groups (six cats/group): control (saline IM), 50-μg/kg Flu IM or intravenously (IV), 100-μg/kg Ati IM or IV, 200-μg/kg Ati IM, 100-μg/kg Ati + 50-μg/kg Flu IM or IV and 200-μg/kg Ati + 50-μg/kg Flu IM. Five …


Seasonal And Antimicrobial Resistance Distributions Of Salmonella Isolated From Pork, Beef And Chicken Meat In Vientiane Capital, Lao Pdr, Sitthasone Xaymountry, Kanit Chukanhom, Seri Klangair, Jatesada Jiwakanon, Sunpetch Angkititrakul Mar 2022

Seasonal And Antimicrobial Resistance Distributions Of Salmonella Isolated From Pork, Beef And Chicken Meat In Vientiane Capital, Lao Pdr, Sitthasone Xaymountry, Kanit Chukanhom, Seri Klangair, Jatesada Jiwakanon, Sunpetch Angkititrakul

The Thai Journal of Veterinary Medicine

The objectives in this study was to investigate the prevalence, seasonal variation and antimicrobial resistance of Salmonella isolated from pork, beef and chicken meat at retail markets in Vientiane capital, Lao PDR. During May 2018 to April 2019, 445 swab samples were randomly collected from pork (n = 258), beef (n = 81) and poultry meat (n = 106) in three seasons at three retail big markets in Vientiane capital, Lao PDR, Thongkhankham, Lao-Aussi, and Nongchan markets. All samples were isolated and identified for Salmonella spp. using the ISO 6579:2002/AMD 1:2007 procedure. The isolates were tested for antimicrobial susceptibility using …