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2019

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Articles 15391 - 15420 of 16114

Full-Text Articles in Medicine and Health Sciences

Prostate Cancer Screening In Thailand: Cost-Effectiveness Analysis And Budget Impact Evaluation, Chavalit Romyen Jan 2019

Prostate Cancer Screening In Thailand: Cost-Effectiveness Analysis And Budget Impact Evaluation, Chavalit Romyen

Chulalongkorn University Theses and Dissertations (Chula ETD)

Objectives: As public health insurance schemes in Thailand did not included prostate cancer screening in their benefits package, this study aimed to systematically gather the effectiveness and explore the cost effectiveness of difference prostate cancer screening programs for the Thai population and find the financial impact for adapting prostate cancer strategy. Method: We perform systematic and network meta-analysis, health economic evaluation using Markov’s model to compare four prostate cancer screening strategies with no screening options. And we perform budget impact analysis to assess the potential burden for adopting selected strategy. Result: The result from systematic review and network meta-analysis suggest …

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Risk Factors For Malaria Infection And Dihydroartemisinin+Piperaquine Resistance Distribution Between High And Low Endemic Areas In Indonesia, Irfanul Chakim Jan 2019

Risk Factors For Malaria Infection And Dihydroartemisinin+Piperaquine Resistance Distribution Between High And Low Endemic Areas In Indonesia, Irfanul Chakim

Chulalongkorn University Theses and Dissertations (Chula ETD)

Globally, malaria case is decreasing over decades. This similar trend happens in Indonesia but it is still not even close to elimination. To control and eradicate malaria require a comprehensive and coincident strategy for the parasite and its vector. However, malaria elimination strategy of many countries, including Indonesia, is still considering only treatment which has led to resistance of Plasmodium species over various antimalarial drugs. Recently, resistance to the only and current available drug, artemisinin, has appeared and spread over continents in the world. However, it has not been extensively discovered in Indonesia. Additionally, it was widely known that a …

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Drug Classification Scheme Review And The Economic Impact Analysis Of Abandoning Non-Prescription Drug Reimbursement In Thailand, Doungporn Leelavanich Jan 2019

Drug Classification Scheme Review And The Economic Impact Analysis Of Abandoning Non-Prescription Drug Reimbursement In Thailand, Doungporn Leelavanich

Chulalongkorn University Theses and Dissertations (Chula ETD)

In Thailand, controversies regarding the drug classification system persist; it was believed that the number of schemes should be changed, and most drugs classified into improper schemes. The system is not also fully used to allocate healthcare budgets, while several other countries cease non-prescription (OTC) drugs reimbursement to allocate this expenditure to catastrophic diseases instead. This study thus aims to (i) review drug classification system in Thailand by comparing to other countries, (ii) evaluate the economic impact of delisting OTC drugs from drug reimbursement list using non-sedating antihistamines (AH) in patients suffering from intermittent allergic rhinitis as a case study, …

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Exploring Alcohol Withdrawal Syndrome And Predicting Alcohol Consumption Change In Patients Admitted To A Genenral Hospitals In Vietnam, Loan Thi Mai Nguyen Jan 2019

Exploring Alcohol Withdrawal Syndrome And Predicting Alcohol Consumption Change In Patients Admitted To A Genenral Hospitals In Vietnam, Loan Thi Mai Nguyen

Chulalongkorn University Theses and Dissertations (Chula ETD)

Risky alcohol drinking is common in hospitalized patients. Early detecting risky alcohol drinkers in hospital is important to provide appropriate management of Alcohol withdrawal syndrome (AWS). This first aim of this study was to identify the extent to which patients admitted to a general hospital in Vietnam meet the criteria for risky alcohol drinker (RAD), alcohol use disorder (AUD), and alcohol withdrawal syndrome (AWS). The second aim was to identify predictors of intension to change and changing alcohol consumption using the Integrated Behavior Model as a conceptual framework. Design: A prospective survey was conducted in Kien An - a general …

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Study Of Pharmacist Workforce In Public Sector, Of Republic Of The Union Of Myanmar, Lwin Nyein Aye Jan 2019

Study Of Pharmacist Workforce In Public Sector, Of Republic Of The Union Of Myanmar, Lwin Nyein Aye

Chulalongkorn University Theses and Dissertations (Chula ETD)

Health workforce shortage was common across the world including Myanmar. However, there was not previous study about the pharmacist workforce to ascertain the shortage problem in Myanmar. This study was aimed to analyze the current situation of pharmacist workforce in Myanmar especially in public sector and to construct a simulation model to suggest the appropriate policy options to the policymakers in improving the pharmacist workforce situations in the public hospitals.For the first objective, the study design was a cross-sectional survey using a mixed method which consisted of the secondary data analysis and semi-structured face-to-face interviews. The government registry data related …

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Precision Medicine Reimbursement Policy Landscape, Nisita Jirawutkornkul Jan 2019

Precision Medicine Reimbursement Policy Landscape, Nisita Jirawutkornkul

Chulalongkorn University Theses and Dissertations (Chula ETD)

Background: Access to precision medicine is limited due to many factors such as precision medicine technology of each country, high-cost technology that requires high finance, limitations on knowledge and competency of personnel and access to precision medicine. Each country manages access to precision medicine differently. The existing evidences of precision medicine reimbursement policy were limited. Objective: To explore reimbursement decision of precision medicine focusing on diagnostic tests and factors associated with reimbursement decision among high-income and upper-middle-income countries. Methods: A targeted review of literatures was conducted through PubMed, MEDLINE, Embase, Cochrane Library, and hand-searching. The study included 13 selected precision …

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Development Of Prediction Model For Chronic Kidney Disease In Type 2 Diabetics In Thailand, Wilailuck Tuntayothin Jan 2019

Development Of Prediction Model For Chronic Kidney Disease In Type 2 Diabetics In Thailand, Wilailuck Tuntayothin

Chulalongkorn University Theses and Dissertations (Chula ETD)

Diabetes mellitus (DM) is a major contributing factor that leads to end stage renal disease (ESRD) with unsuitable clinical management. Chornic kidney disease (CKD) prediction model could prevent the progression of CKD to ESRD. However, current CKD prediction models in patients with type 2 DM were developed to predict ESRD. This study aimed to develop prediction model for CKD stage 3 in patients with type 2 DM in Thailand. This was a 10-year retrospective cohort study obtaining data of patients with type 2 DM from electronic database of Taksin hospital during 1 January 2008 to 31 December 2017. The outcome …

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Situation Analysis Of Drug Product Information Provision In Yangon And Review On Consumer Drug Information Guidelines Across Countries, Zar Ni Win Jan 2019

Situation Analysis Of Drug Product Information Provision In Yangon And Review On Consumer Drug Information Guidelines Across Countries, Zar Ni Win

Chulalongkorn University Theses and Dissertations (Chula ETD)

The appropriate drug use and successful treatment relies heavily on how patients administer medicines by themselves and thus healthcare knowledge and drug information become crucial for them. Firstly, content analysis was conducted to evaluate drug product information attached with the 30 drug samples of three drug classes. To evaluate written and verbal drug information, standardized simulated patient survey was conducted by using Mefenamic Acid provided in community pharmacies around Yangon. In the second step of this study, targeted literature review was performed to identify and compare different regulations of PILs and PIL development guidelines in terms of contents, designs and …

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Single Fixed-Time Artificial Insemination With Fresh Or Frozen Thawed Semen In Pig, Pachara Pearodwong Jan 2019

Single Fixed-Time Artificial Insemination With Fresh Or Frozen Thawed Semen In Pig, Pachara Pearodwong

Chulalongkorn University Theses and Dissertations (Chula ETD)

Single fixed-time AI is the breeding protocol inseminating the sows once with either fresh or frozen-thawed (FT) semen at fixed time after ovulation induction without estrus detection. To develop the success single fixed-time AI protocol in Thailand, factors affecting the efficacy of single fixed-time AI were evaluated. Additionally, we also evaluated the effect of using lecithin in cryopreservation extender on FT semen qualities in order to find the possibility to perform single fixed-time AI using sperm preserved in lecithin based extender. It was found that administration of 10 ug buserelin at 72 h after weaning decreased weaning-to-ovulation interval compared to …

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Development Of An Indirect Elisa To Detect Antibodies Against Actinobacillus Suis Using Boiled Whole Cell Antigen, Siriluck Juntautsa Jan 2019

Development Of An Indirect Elisa To Detect Antibodies Against Actinobacillus Suis Using Boiled Whole Cell Antigen, Siriluck Juntautsa

Chulalongkorn University Theses and Dissertations (Chula ETD)

Actinobacillus suis is considered an opportunistic pathogen in swine that can cause disease in animals of all ages, especially in high health status herds. The objective of this study was to developed an indirect enzyme-linked immunosorbent assay (ELISA) using boiled whole cell antigen for the detection of antibody against A. suis. The selected herd has clinical problems associated with A. suis infection. In addition, this herd was not received any Actinobacillus pleuropneumoniae (APP) vaccine nor diagnosed with APP infection before the study. The isolation of A. suis was recovered from lung of the infected pig in the selected herd. Moreover, …

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Short Term Effects Of Sildenafil In Treatment Of Pulmonary Hypertension In Degenerative Mitral Valve Disease Dogs, Karun Saetang Jan 2019

Short Term Effects Of Sildenafil In Treatment Of Pulmonary Hypertension In Degenerative Mitral Valve Disease Dogs, Karun Saetang

Chulalongkorn University Theses and Dissertations (Chula ETD)

Degenerative mitral valve disease (DMVD) is the most common acquired cardiac disease in small breed dogs. Pulmonary hypertension (PH) is a common complication in DMVD that can worsen the clinical signs, cardiac function, and median survival time of DMVD dogs. Conventional therapy of DMVD can reduce systolic pulmonary arterial pressure (sPAP) minimally. Thus, additional therapy is required. Sildenafil is used to treatment of PH in various causes, but the effect of sildenafil in the treatment of PH secondary to DMVD is not published yet. Therefore, this study aimed to evaluate the effects of sildenafil in combination with conventional therapy in …

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The Invention Of Plastic Plate For The Semiquantitative Detection Of Somatic Cells In Farm Bulk Tank Milk, Piyarat Jiarpinitnun Jan 2019

The Invention Of Plastic Plate For The Semiquantitative Detection Of Somatic Cells In Farm Bulk Tank Milk, Piyarat Jiarpinitnun

Chulalongkorn University Theses and Dissertations (Chula ETD)

Bulk tank milk somatic cell count (BTM SCC) is worldwide acceptable as an indicator of subclinical mastitis and milk quality at the farm level. Several countries determine the BTM SCC level for quality control and milk pricing. In Thailand, BTM SCC standard is set at 500,000 cells/ml and premium milk quality is set at less than 100,000 cells/ml. Milk collecting centers (MCCs) use CMT (California Mastitis Test) for screening raw milk quality. However, CMT has an error and high variation. This study aims to invent a new semiquantitative device for determining BTM SCC and to validate the device in comparison …

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Assessment Of Left Atrial Function In Feline Hypertrophic Cardiomyopathy By Using Two Dimensional Speckle Tracking Echocardiography, Arisara Kiatsilapanan Jan 2019

Assessment Of Left Atrial Function In Feline Hypertrophic Cardiomyopathy By Using Two Dimensional Speckle Tracking Echocardiography, Arisara Kiatsilapanan

Chulalongkorn University Theses and Dissertations (Chula ETD)

Hypertrophic cardiomyopathy (HCM) is one of the most common myocardial diseases in cats. The left atrial (LA) function plays an essential role in compensatory mechanism of left ventricle with diastolic dysfunction to maintain cardiac output in cats affected with HCM. Nowadays, there are several echocardiographic techniques used to evaluate the left atrial function; however, each technique has limitations. To overcome the limitations, two-dimensional speckle tracking echocardiography (2D-STE) is a novel technique for assessment the LA function. To date, no study focusing on assessment of the left atrial function by 2D-STE in feline HCM has been reported. The objective of this …

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Vector Competence Of Culex Tritaeniorhynchus For Duck Tembusu Virus, Jitra Sanisuriwong Jan 2019

Vector Competence Of Culex Tritaeniorhynchus For Duck Tembusu Virus, Jitra Sanisuriwong

Chulalongkorn University Theses and Dissertations (Chula ETD)

Duck Tembusu virus (DTMUV), an emerging infectious disease in ducks, belongs to the Ntaya virus group of the Flavivirus genus in the Flaviviridae family. The emergence of DTMUV has been observed in layer and breeder duck farms in China since 2010 and in layer and broiler duck farms in Thailand since 2013. Infected ducks show neurologic signs, including an incapability to stand, ataxia, and paralysis. A significant drop in egg production is usually observed among layer ducks. The transmission of DTUMV involves mosquito vectors, however, the exact role of mosquitoes in the ecology of DTMUV in Thailand remains unclear. The …

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Development Of Mucoadhesive Nanovaccine Against Columnaris Disease In Red Tilapia (Oreochromis Sp.), Sirikorn Kitiyodom Jan 2019

Development Of Mucoadhesive Nanovaccine Against Columnaris Disease In Red Tilapia (Oreochromis Sp.), Sirikorn Kitiyodom

Chulalongkorn University Theses and Dissertations (Chula ETD)

Tilapia (Oreochromis sp.) is very important and high production of freshwater fish in Thailand. Columnaris disease has been now recognized as one of the most serious infectious diseases in farmed tilapia. The disease is caused by Flavobacterium columnare. Among the prevention and control strategies, vaccination is one of the most effective approach. According to the pathogenesis of this bacteria, the characteristic lesion is almost at mucosal area of skin and gill. Therefore, hypothesized that the mucosal nanovaccine with mucoadhesive characteristic could be suitable vaccination method to control columnaris disease. In this study, we determined vaccine strain candidate by clinical field …

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Epidemiological Surveillance Of Feline Morbillivirus Infection In The Aspects Of Polymerase Chain Reaction, Indirect Elisa And Immunohistochemistry, Surangkanang Chaiyasak Jan 2019

Epidemiological Surveillance Of Feline Morbillivirus Infection In The Aspects Of Polymerase Chain Reaction, Indirect Elisa And Immunohistochemistry, Surangkanang Chaiyasak

Chulalongkorn University Theses and Dissertations (Chula ETD)

Feline morbillivirus (FeMV), a relatively new virus, has been discovered in domestic cats and associated with kidney diseases since 2012 in Hong Kong. The FeMV study gained widely attentions in many countries whereas the pathogenesis remains elusive. These studies aimed to provide the FeMV knowledge in Thai domestic cat by performing the genetic-based, serological-based and pathological-based studies of FeMV-infected in Thai cats. Additionally, the indirect enzyme linked immunosorbent assay (ELISA) and rabbit polyclonal antibody against FeMV matrix (FeMV-M) protein was produced for seroprevalence and viral localization by immunohistochemistry (IHC) techniques, respectively. Our results revealed the FeMV RNA prevalence at 11.9% …

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Evidence Of Vertical And Horizontal Transmission Of Francisella Noatunensis Subsp. Orientalis In Hybrid Red Tilapia (Orerochromis Sp.), Vuong Viet Nguyen Jan 2019

Evidence Of Vertical And Horizontal Transmission Of Francisella Noatunensis Subsp. Orientalis In Hybrid Red Tilapia (Orerochromis Sp.), Vuong Viet Nguyen

Chulalongkorn University Theses and Dissertations (Chula ETD)

Francisellosis caused by Franciscella orientalis (formerly named: Franciscella noatunensis subspecies orientalis) has been reported as one of the most important emergent diseases of warm water fish. However, little is known about its transmission, fastidious nature, survival, and persistence in multiple environments and global presence. This study aims to investigate vertical transmission, horizontal transmission by vectors and effect of other organisms on the outbreak of francisellosis in hybrid red tilapia. To prove the transmission of F. orientalis from subclinically infected tilapia mouthbrooders to their offspring through the current practice of fry production in tilapia hatcheries, experimentally infected hybrid red tilapia broodstock …

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Detection On The Expression Of The Transcription Factors: Oct-4, Nanog, And Sox-2 Genes In Canine Cutaneous Mast Cell Tumors, Sirilak Meesuwan Jan 2019

Detection On The Expression Of The Transcription Factors: Oct-4, Nanog, And Sox-2 Genes In Canine Cutaneous Mast Cell Tumors, Sirilak Meesuwan

Chulalongkorn University Theses and Dissertations (Chula ETD)

The objectives of this study were to determine the expression of Oct-4, Nanog, and Sox-2 in both mRNA and proteins levels. The 30 biopsied MCT cases, from the Small Animal Teaching Hospital, Faculty of Veterinary Science, Chulalongkorn University, were used in this study. The samples were divided into two parts; part I, the biopsied samples were processed for paraffin section and stained with H&E stain, for grading based on Patnaik’s system and Kiupel’s system and the other part was detected the expression of Oct-4, Nanog, and Sox-2 genes. The MCT samples were classified into three grades; grade I, II, and …

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Correlation Of Allergen Specific Immunoglobulin To House Dust Mites And Commensal Microbes In Atopic Dogs And Identification Of Major Allergen In Dermatophagoides Farinae, Nathrada Khantavee Jan 2019

Correlation Of Allergen Specific Immunoglobulin To House Dust Mites And Commensal Microbes In Atopic Dogs And Identification Of Major Allergen In Dermatophagoides Farinae, Nathrada Khantavee

Chulalongkorn University Theses and Dissertations (Chula ETD)

To confirm a causative allergen in atopic dogs could test through intradermal skin testing (IDT) or allergen-specific IgE serology testing (ASIS). ASIS is more practical, convenient and safer. However, the results of ASIS could be affected by source and type of allergen. Crude allergen extracts were still used in commercial testing and protein antigens of skin microbes were not included in the panel of testing. In this study, our group developed serology test for confirming house dust mite allergy called Dermatophagoides farinae and D. pteronyssinus, and measuring specific immunoglobulin to skin microbes on dogs like Malssezia pachydermatis yeast and Staphylococcus …

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Risk Assessment Of Vibrio Parahaemolyticus In White Shrimp (Litopenaeus Vannamei) From Retail Market, Praphatsorn Yokyingyong Jan 2019

Risk Assessment Of Vibrio Parahaemolyticus In White Shrimp (Litopenaeus Vannamei) From Retail Market, Praphatsorn Yokyingyong

Chulalongkorn University Theses and Dissertations (Chula ETD)

Vibrio parahaemolyticus is the leading cause of seafood borne gastroenteritis in many countries including Thailand. It is a gram-negative, halophilic bacterium which was normally found in estuarine environment and seafood. The infection usually occurred from consuming raw, inadequate cooked, or cross-contaminated seafood. Risk assessment is a scientific tool to evaluate health hazard from V. parahaemolyticus infection. In this study, a risk of V. parahaemolyticus infection from raw shrimp consumption (Litopenaeus vannamei) was conducted. This study estimated the prevalence and level of V. parahaemolyticus in 2 retail types across 6 provinces nationwide by 3-tubes MPN. Risk assessment from raw shrimp consumption …

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Monitoring Of Influenza A Viruses In Thailand And Pathogenicity In Dogs And Guinea Pigs, Ratanaporn Tangwangvivat Jan 2019

Monitoring Of Influenza A Viruses In Thailand And Pathogenicity In Dogs And Guinea Pigs, Ratanaporn Tangwangvivat

Chulalongkorn University Theses and Dissertations (Chula ETD)

Influenza A virus causes respiratory disease in many species such as birds, horses, pigs, dogs and cats as well as humans. This thesis consists of 5 phases. Phases 1 was monitoring of canine influenza virus infection in Thailand. Phase 2 was determining the intravenous pathogenicity index of Thai canine influenza virus. Phase 3, 4 and 5 were investigating the pathogenicity of canine influenza viruses in dogs, chickens and guinea pigs, respectivly. Our results showed that 0.97% of canine serum samples (9/932) and 1.20% of feline serum sample (1/79) were tested positive for influenza A antibodies by NP-ELISA. Six serum samples …

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Genetic Characteristics Of Avian Influenza A Viruses Isolated From Thai Free-Grazing Ducks And Their Pathogenicity Potential In Animal Models, Supassama Chaiyawong Jan 2019

Genetic Characteristics Of Avian Influenza A Viruses Isolated From Thai Free-Grazing Ducks And Their Pathogenicity Potential In Animal Models, Supassama Chaiyawong

Chulalongkorn University Theses and Dissertations (Chula ETD)

Influenza A viruses (IAVs) can infect several animal species and humans. In Thailand, free-grazing ducks are reservoir hosts of influenza A virus. The free-grazing ducks are potentially interface with wild birds and domestic birds and subsequently cause interspecies transmission of IAVs. This thesis composes of three study phases. Phase 1 is surveillance of influenza A in free-grazing ducks. Phase 2 is genetic characterization and diversity analysis of influenza A viruses from free-grazing ducks. Phase 3 is challenge of influenza A viruses isolated from free-grazing ducks in animal models. For phase 1, multi-years surveillance of IAVs in free-grazing ducks was conducted …

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Detection And Study Of Genetic Diversity Of Selected Vector-Borne Protozoa And Rickettsia In Buffaloes In Thailand By Biomolecular Method, Hoang Lan Anh Nguyen Jan 2019

Detection And Study Of Genetic Diversity Of Selected Vector-Borne Protozoa And Rickettsia In Buffaloes In Thailand By Biomolecular Method, Hoang Lan Anh Nguyen

Chulalongkorn University Theses and Dissertations (Chula ETD)

This study aimed to determine the occurrence, geographical distribution and genetic diversity of tick and mosquito-borne protozoa and rickettsia in buffaloes by the PCR – sequencing approach. A total of 456 blood samples were collected in provinces of Thailand including Mukdahan, Uthai Thani, Lampang, Amnat Charoen, Nong Bua Lamphu, Phatthalung, Surin and Chachoengsao. DNA was extracted from buffalo blood and screened for the presence of pathogens, followed by sequencing. The results indicated that the overall prevalence of rickettsia, protozoa, and malaria were 41%, 25.4%, and 12.9% respectively. Double and triple infection of buffaloes with pathogens were observed in 19.1% of …

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Genetic Characterization Of Canine Parvovirus And Circovirus In Dogs In Vietnam, Nguyen Manh Tuong Jan 2019

Genetic Characterization Of Canine Parvovirus And Circovirus In Dogs In Vietnam, Nguyen Manh Tuong

Chulalongkorn University Theses and Dissertations (Chula ETD)

Canine parvovirus (CPV-2) emerged and caused severe enteritis in dogs since the 1970s. The last variant, CPV-2c is considered as the high virulent strain and spread ubiquitous worldwide. This strain was early detected in Vietnam since 2002, but the whole genome is still fragmentary. In this study, we attempted to gain the whole genome of CPV-2c from the Vietnamese infected dogs as the first report of the full-length CPV-2c sequence originated from Asia. The Vietnamese CPV-2c exhibited the common mutations in both structural and nonstructural proteins and shared a typical evolutionary pattern with Asian strains. Besides, Vietnamese sequences presented some …

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Effect Of Fish Antifreeze Protein Type Iii Supplementation To Semen Extender On Cryopreserved Dog Spermatozoa, Rudal Jetta Bayusalaksa Jan 2019

Effect Of Fish Antifreeze Protein Type Iii Supplementation To Semen Extender On Cryopreserved Dog Spermatozoa, Rudal Jetta Bayusalaksa

Chulalongkorn University Theses and Dissertations (Chula ETD)

Ice binding protein such as antifreeze protein (AFP) type III have been used as a cryoprotectant and proven to improve sperm survival in some species but dog. Addition of Equex STM paste to semen freezing medium provides beneficial effects on cryopreserved dog sperm. Hence, the objectives of this study were to (a) evaluate the effects of AFP (Experiment I) and (b) its combination with Equex STM paste on cryopreserved dog spermatozoa (Experiment II). The semen samples were pooled to allow for a sufficient number of sperm and to reduce an individual variation. Only sperm-rich fractions with total motility more than …

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Establishment Of Encapsulated Human Dental Pulp Stem Cell-Derived Insulin Producing Cells (Hdpsc-Ipcs) For Translational Stem Cell Based Diabetes Therapy, Suryo Kuncorojakti Jan 2019

Establishment Of Encapsulated Human Dental Pulp Stem Cell-Derived Insulin Producing Cells (Hdpsc-Ipcs) For Translational Stem Cell Based Diabetes Therapy, Suryo Kuncorojakti

Chulalongkorn University Theses and Dissertations (Chula ETD)

Current approach for diabetes treatment remained several adverse events varied from gastrointestinal to life-threatening symptoms. Regenerative therapy regarding Edmonton protocol has been facing serious limitations involving protocol efficiency and safety. This led to the study for alternative insulin-producing cell (IPC) resource and transplantation platform. In this study, evaluation of encapsulated human dental pulp stem cell (hDPSC)-derived IPCs (hDPSC-IPCs) by alginate (ALG) and pluronic F127-coated alginate (ALGPA) was performed. The results showed that ALG and ALGPA preserved hDPSC viability and allowed glucose and insulin diffusion in and out. ALG- and ALGPA-encapsulated hDPSC-IPCs maintained viability for at least 336 hours and sustained …

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Detection And Differentiation Of Haemosporidian Parasites In Chickens (Gallus Gallus Domesticus) Using Molecular Diagnostic Approach, Thi Xuan Mai Nguyen Jan 2019

Detection And Differentiation Of Haemosporidian Parasites In Chickens (Gallus Gallus Domesticus) Using Molecular Diagnostic Approach, Thi Xuan Mai Nguyen

Chulalongkorn University Theses and Dissertations (Chula ETD)

Haemosporidioses in domestic chicken (Gallus gallus domesticus) are not only widely distribute but causing high mortality and non-specific symptoms. The multiplex PCR assay developed in this study can concurrently detect and differentiate four haemosporidian parasites Leucocytozoon caulleryi, Leucocytozoon sabrazesi, Plasmodium juxtanucleare, and Plasmodium gallinaceum. Comparing to microscopic examination performed in parallel on 157 samples, multiplex PCR assay exhibited significantly higher detection rate in diagnosis of haemosporidians (p = 0.000). The results showed that, L. sabrazesi, P. juxtanucleare, and P. gallinaceum detection rate of multiplex PCR were all higher than that of microscopic examination with p = 0.002, 0.000, and 0.004, …

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Resistome Analysis Of Aeromonas Veronii Strains Isolated From Diseased Outbreak In Tilapia Farms In Thailand, Rungnapa Sakulworakan Jan 2019

Resistome Analysis Of Aeromonas Veronii Strains Isolated From Diseased Outbreak In Tilapia Farms In Thailand, Rungnapa Sakulworakan

Chulalongkorn University Theses and Dissertations (Chula ETD)

Aeromonas veronii outbreaks in tilapia farming caused relatively high mortality. Moreover, it was resistant to many kinds of antimicrobial used in Thailand aquaculture. According to no CLSI standard, the determination of antimicrobial efficacy has been limited phenotypically; the genomics study is required. This research aims to analyze the resistome of A. veronii isolated from diseased Tilapia in Chainat, Nong Khai and Uttaradit province, Thailand. Twelve isolates of A. veronii were identified base on gyrB sequencing then determined the MIC value to eight antimicrobials (AMP, AML, GEN, ENR, OXO, OXY, SXT, and FFC). According to MIC patterns, five representatives were performed …

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Chondroprotective Efficacy Of Undenatured Collagen Type Ii On Canine Osteoarthritis Secondary To Medial Patellar Luxation, Susolos Kunsuwanachai Jan 2019

Chondroprotective Efficacy Of Undenatured Collagen Type Ii On Canine Osteoarthritis Secondary To Medial Patellar Luxation, Susolos Kunsuwanachai

Chulalongkorn University Theses and Dissertations (Chula ETD)

Osteoarthritis (OA) is found secondary from medial patellar luxation (MPL) which is one of the most common diseases in small-breed dogs. Managements of the disease are surgical correction or a medical treatment. In the patients who are unable to do the surgical correction, non-steroidal anti-inflammatory drugs (NSAIDs) remains the drug of choice for OA therapy. Nowadays nutraceuticals have become more popular since there are no side effects once given for a long period of time. The purpose of this study was to evaluate the clinical chondroprotective effects of undenatured collagen type-II (UCII) on OA secondary to canine medial patellar luxation. …

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Comparison Of The Physical Capacity Using 6-Minute Walk Test And Questionnaire Before And After Resection Of Elongated Soft Palate By Bipolar Sealing Device In French Bulldogs, Tamolwan Anyamaneecharoen Jan 2019

Comparison Of The Physical Capacity Using 6-Minute Walk Test And Questionnaire Before And After Resection Of Elongated Soft Palate By Bipolar Sealing Device In French Bulldogs, Tamolwan Anyamaneecharoen

Chulalongkorn University Theses and Dissertations (Chula ETD)

Brachycephalic airway obstruction syndrome (BAOS) is an anatomical abnormality of upper airway commonly found in brachycephalic breeds leading to clinical signs and physical capacity impairment. The purposes of this study were to compare clinical signs and physical capacity in French bulldogs between before and after surgical correction using questionnaire and 6-minute walk test (6-MWT). Twenty-seven French bulldogs were enrolled in this study and divided into normal group (n=7) and BAOS group (n=20). History taking, clinical signs and questionnaire information were recorded and 6-MWT was performed in both groups. BAOS dogs had significantly higher severity of clinical signs from questionnaire with …

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