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2018

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Articles 14701 - 14730 of 15422

Full-Text Articles in Medicine and Health Sciences

The Village Based Malaria Elimination Model For Interrupting Malaria Transmission In Banmauk Township, Myanmar, Pyae Linn Aung Jan 2018

The Village Based Malaria Elimination Model For Interrupting Malaria Transmission In Banmauk Township, Myanmar, Pyae Linn Aung

Chulalongkorn University Theses and Dissertations (Chula ETD)

Different projects engage to eliminate malaria in Myanmar; however, malaria continues to remain a significant public health problem. Therefore, a comprehensive package intervention called "the village-based malaria elimination model" was carried out in one of the malaria-affected townships, Banmauk, in upper Myanmar, during June to November 2018. It is a systemic intervention to address interrupting of malaria transmission in accordance with elimination definition (i.e <1/1000 API) by means of comprehensive malaria control activities which consists of preliminary entomology assessment and KAP survey, routine mass blood survey, assessing diagnostic accuracy of current tools, loudspeakers base health messages announcements, real-time cases reporting and notification through mobile messaging by village malaria workers. Four villages among the most malariaburdened areas were randomly selected: two villages were assigned as the intervention group and two as the control group. Each activity included in the model showed separate effective outcomes. Overall, there were significant improvements in community practice related to malaria care-seeking. In-time reporting systems through mobile messaging were feasible and, more importantly, there was an overall effect in reducing malaria morbidity. In the linear regression model, the factors demonstrated to be significant predictors of decline in malaria by intervention villages were the number of health message announcement activities (p < 0.001), real-time mobile case reporting by malaria workers (p < 0.001), ownership of nets (p < 0.001), and routine mass blood survey (p < 0.05). However, the training showed no statistically significant association (p > 0.05). The steep decline in malaria cases among the intervention villages suggested that this intervention might be effective to reduce the malaria burden among these rural locales. The National Malaria Control Program may consider implementing this …

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Acute Symptoms Arising From Application Of Herbicides Among Rice Farmers In Song Phi Nong Subdistrict, Song Phi Nong District, Suphan Buri Province, Thailand: A Cross-Sectional Survey, Qin Cai Jan 2018

Acute Symptoms Arising From Application Of Herbicides Among Rice Farmers In Song Phi Nong Subdistrict, Song Phi Nong District, Suphan Buri Province, Thailand: A Cross-Sectional Survey, Qin Cai

Chulalongkorn University Theses and Dissertations (Chula ETD)

Agriculture is a primary contributor to the Thailand economy. In order to high production, pesticide use is necessary and herbicides are the first import volume of pesticides. Meanwhile overuse of herbicide can cause adverse health effects such as acute symptoms even death, especially glyphosate, paraquat, and 2-4 D. The aim of this study is to explore acute symptoms arising from application of herbicides and to find factors associated to symptoms of herbicide exposure on rice farmers in Song Phi Nong subdistrict, Song Phi Nong district, SuphanBuri province, Thailand. A cross-sectional study was investigated 133 participants through face to face interview. …

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Socioeconomic Inequality In Relation To Oral Health Among Thai Population : A Secondary Data Analysis, Sirinthip Amornsuradech Jan 2018

Socioeconomic Inequality In Relation To Oral Health Among Thai Population : A Secondary Data Analysis, Sirinthip Amornsuradech

Chulalongkorn University Theses and Dissertations (Chula ETD)

Since major oral health problems include dental caries, periodontal disease and tooth loss. Studies from many countries reports inequality in oral health which has become challenging worldwide. People with lower socioeconomic position prone to have more severe condition in oral health more than those with higher position. However, there are few studies on socioeconomic inequality in oral health in developing countries, including Thailand. This study aimed to determine the relationship of socioeconomic difference in oral health outcome and oral health behavior among Thai adult population. This study is a cross-sectional analytical study using secondary data from the 7th Thailand National …

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Prevalence And Associated Factors Of Neonatal Microcephaly In Thailand 2014-2018, Tassana Thammaros Jan 2018

Prevalence And Associated Factors Of Neonatal Microcephaly In Thailand 2014-2018, Tassana Thammaros

Chulalongkorn University Theses and Dissertations (Chula ETD)

The concern of neonatal microcephaly became high after Zika outbreaks occurred worldwide. Thailand was one of the affected countries. The estimation of neonatal microcephaly prevalence was crucial for public health preparedness and response. The epidemiological characteristics of microcephaly specifically to Thailand were vital to developing clinical management and guideline. The objectives of this study were to estimate the prevalence, describe the epidemiological characteristic and identify associated factors of neonatal microcephaly in Thailand during 2014-2018. This study was a cross-sectional study using data from the health data center, ministry of public health Thailand, 69% of live birth in Thailand was included …

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The Influence Of Indoor Air Pollution Sources On Respiratory Health Of Occupants In Offices : A Cross-Sectional Study At Suan Sunandha Rajabhat University, Bangkok, Thailand, Thammarak Srimarut Jan 2018

The Influence Of Indoor Air Pollution Sources On Respiratory Health Of Occupants In Offices : A Cross-Sectional Study At Suan Sunandha Rajabhat University, Bangkok, Thailand, Thammarak Srimarut

Chulalongkorn University Theses and Dissertations (Chula ETD)

Background: Indoor air pollution contains several substances and can emanate from a range of sources. In particular importance might be substances known as volatile organic compounds and respirable particulates. The exposure to indoor air pollution can induce a wide range of acute and chronic respiratory health effects. The study's aims were to identify the effect of indoor air pollution sources and concentrations in offices on respiratory health of occupants and identify potential factors that may be related to respiratory health problems. Methods: Fourteen offices were measured the concentration of PM2.5 and TVOC at 1.20 meters high for 8 hours and …

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Delay In Health Seeking Behavior Among Tb Patients In Chiang Rai Province, Thailand, Yotsanon Sikkhajan Jan 2018

Delay In Health Seeking Behavior Among Tb Patients In Chiang Rai Province, Thailand, Yotsanon Sikkhajan

Chulalongkorn University Theses and Dissertations (Chula ETD)

The aim of this study was to determine the pattern of health seeking behavior and factors associated with delay in health seeking behavior among tuberculosis patients in border hospitals, Chiang Rai province, Thailand. A cross-sectional study was conducted in four biggest border hospitals in Chiang Rai province, Thailand during May to July 2018 among 103 identified tuberculosis cases. Data was collected by a structured questionnaire on patients' general characteristics, HIV status, distance from health service, health seeking treatment with onset symptoms, and patient delay. Collected data was analyzed in SPSS 22. Both descriptive and inferential statistics was employed in data …

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Trends And Factors Associated With Breastfeeding In Thailand : A Secondary Analysis Of The Multiple Indicator Cluster Survey 2005-2016, Yuanpei Chen Jan 2018

Trends And Factors Associated With Breastfeeding In Thailand : A Secondary Analysis Of The Multiple Indicator Cluster Survey 2005-2016, Yuanpei Chen

Chulalongkorn University Theses and Dissertations (Chula ETD)

This cross-sectional study was based on secondary data of Thailand Multiple Indicator Cluster Survey (MICS round 3, 4 and 5) between 2005 and 2016 and aimed to identify trends and factors associated with breastfeeding in Thailand. Subjects were selected from the three MICS surveys and then pooled together to form final weighted samples of 9757 children under 2 years for studying early initiation of breastfeeding and 2568 children under 6 months for exclusive breastfeeding. Univariate and multivariate logistic regression analyses were applied to identify trends and factors. Results indicated that the overall breastfeeding rates were low in spite of an …

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Effectiveness Of Thai Hermit Exercise (Ruesi Datton) On Mild Cognitive Impairment's Patients, Chomlak Kongart Jan 2018

Effectiveness Of Thai Hermit Exercise (Ruesi Datton) On Mild Cognitive Impairment's Patients, Chomlak Kongart

Chulalongkorn University Theses and Dissertations (Chula ETD)

Mild Cognitive Impairment (MCI) is a recurrent brain disorder in memory. It is caused by the degeneration of the brain cells that is different from an individual's age and develop to dementia. One way to help restore memory is to exercise. Thai hermit exercise is a wisdom in Thai exercise, which improve memory decline for the condition. The study design is randomized control trial. This study aimed to investigate the effectiveness of Thai hermit exercise among patients with MCI. 84 participants were recruited and randomized into intervention (n=42) control(n=42). The intervention group practiced Thai hermit exercise for 10 weeks. Psychological …

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Health Policies And Programs Facilitating Access To High-Cost Anticancer Drugs, Chanthawat Patikorn Jan 2018

Health Policies And Programs Facilitating Access To High-Cost Anticancer Drugs, Chanthawat Patikorn

Chulalongkorn University Theses and Dissertations (Chula ETD)

Access to anticancer drugs is limited mainly due to their high cost. To support policymakers in Thailand to develop policies and programs to facilitate better access to high-cost anticancer drugs, global and local evidence are needed. A systematic review of literature was conducted using PubMed, Embase and Web of Science between October 8-11, 2018 to identify peer-reviewed articles published in English from 2000 to 2018. Studies were included if they described health policies or programs facilitating access to high-cost anticancer drugs. Using thematic synthesis, policies and programs were summarized by themes and by income classification of countries. In addition, a …

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Pharmacognostic Specification And Brazilin Content In Caesalpinia Sappan Heartwoods, Macroscopic, Microscopic And Molecular Identification Of Selected Caesalpinia Species In Thailand, Suwipa Intakhiao Jan 2018

Pharmacognostic Specification And Brazilin Content In Caesalpinia Sappan Heartwoods, Macroscopic, Microscopic And Molecular Identification Of Selected Caesalpinia Species In Thailand, Suwipa Intakhiao

Chulalongkorn University Theses and Dissertations (Chula ETD)

The genus Caesalpinia L. belongs to Caesalpiniaceae family. This genus was spread throughout Asia and South East Asia. In Thailand, 18 species of Caesalpinia L. genus were reported which were spread in every parts. Due to the similar morphology especially the leaf part and closely vernacular name of eight Caesalpinia species, the identification of these species is complicated. Caesalpinia sappan L. is the well-known species which has many important drug properties. It was used as food, beverage, and cosmetic industries especially the heartwood part that contained brazilin, one of the flavonoid group. This study aimed to establish the pharmacognostic specification, …

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Needs Analysis And Development Of Professional Development Program For Thai Community Pharmacists, Surangkana Puengrung Jan 2018

Needs Analysis And Development Of Professional Development Program For Thai Community Pharmacists, Surangkana Puengrung

Chulalongkorn University Theses and Dissertations (Chula ETD)

Background: Primary care services, as recommended by the National Health Security Office (NHSO), are crucial for Thai community pharmacists. Training needs for the services and a professional development or training program however remained unexplored. Objectives: To analyze community pharmacists' training needs and develop professional development programs to meet their needs. Methods: A mixed-methods study with two phases was conducted during 2018-2019. Phase 1 was involved in the needs assessment using semi-structured interview with 10 key informants who were community pharmacists and stakeholders based on three levels (i.e. tasks, person, and organization) and needs confirmation with a self-administered questionnaire surveyed in …

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The Effect Of Recovery Combination On Muscle Oxygenation, Blood Lactate Concentration, And Subsequent Performance During 200-M Repeated Swimming, Ade Bagus Pratama Jan 2018

The Effect Of Recovery Combination On Muscle Oxygenation, Blood Lactate Concentration, And Subsequent Performance During 200-M Repeated Swimming, Ade Bagus Pratama

Chulalongkorn University Theses and Dissertations (Chula ETD)

This study aimed to compare the effectiveness of three recovery protocols on muscle oxygenation, blood lactate, and subsequent performance during a 200-m repeated swimming. Twelve swimmers (6 females and 6 males) from Chulalongkorn University swimming club completed three sessions of two consecutive 200-m front crawl trials separated by one of three recovery protocols: a 15-min of active recovery (AR), a 15-min of passive recovery (PR), and a recovery combination of 5-min of active and 10-min of passive recovery (CR) by using a counterbalance design. Skeletal muscle oxygenation, blood lactate concentration (BL), arterial oxygen saturation (SaO2), and heart rate (HR) were …

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Innovation Of Gonadotropin Releasing Hormone (Gnrh)-Modified Nanocarrier Delivered To Induce Testicular And Mammary Cell Apoptosis, Chatwalee Boonthum Jan 2018

Innovation Of Gonadotropin Releasing Hormone (Gnrh)-Modified Nanocarrier Delivered To Induce Testicular And Mammary Cell Apoptosis, Chatwalee Boonthum

Chulalongkorn University Theses and Dissertations (Chula ETD)

The main overall goal of this study is to investigate the application of modified chitosan as a potential vector for apoptotic gene delivery to gonadotropin-releasing hormone receptor (GnRHR)-expressing cells (i.e. testicular cells and mammary cancer cells). This study reported Gonadotropin Releasing Hormone-modified Chitosan (GnRH-CS) nanoparticle as a promising vector for targeted gene delivery, and a GnRH peptide was used for active targeting of a transgene in GnRHR expressing cells. The physiological characterizations of the prepared nanoparticle were investigated by proton nuclear magnetic resonance spectroscopy (1H NMR), Attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR) and Nanosizer. The prepared GnRH-CS was …

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Validation Of Lamp-Lfd Ehrlichia Canis Dna Sensor Kit, Pipat Jirapiti Jan 2018

Validation Of Lamp-Lfd Ehrlichia Canis Dna Sensor Kit, Pipat Jirapiti

Chulalongkorn University Theses and Dissertations (Chula ETD)

Diagnosis of Canine Monocytic Ehrlichiosis is in light of anamesis, visualized cytoplasmic morulae, clinical signs and laboratory test result of platelet counts. The PCR technique is always a definitive confirmatory test of Ehrlichia canis infection. However, the drawback of PCRs test is crucially needed of laboratory equipments. This drawback is somehow enabled newly DNA based technology to be developed in order to eliminate laborious thermocycler and gel electrophoresis. The LAMP-LFD is on focus by using a single temperature to amplify DNA and no gel electrophoresis involved to detect DNA product. LAMP-LFD is included in DNAsensor test kit that recently developed …

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The Effect Of Monosodium Glutamate On Brain, Liver, Kidney, Testis, Ovary, Fertilization And Hatching Rate In Zebrafish (Danio Rerio), Chortip Sajjaviriya Jan 2018

The Effect Of Monosodium Glutamate On Brain, Liver, Kidney, Testis, Ovary, Fertilization And Hatching Rate In Zebrafish (Danio Rerio), Chortip Sajjaviriya

Chulalongkorn University Theses and Dissertations (Chula ETD)

Monosodium glutamate (MSG) is composed of sodium salt and amino acids that is commonly used to enhance food taste. Although it is safe at consumption level, the adverse effect of MSG had been reported. The present research was intended to evaluate the effect of MSG on the brain, liver, kidney, testes, and ovaries of zebrafish (Danio rerio) which were exposed from embryo stage to adult stage, along with the analyzing of the fertilization rate and hatching rate of zebrafish embryos. From the observation of histopathological changing of different organs of zebrafish after exposed to 10, 100, and 1000 ppm MSG …

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Synbiotic Effects Of Jerusalem Artichoke (Helianthus Tuberosus) And Lactobacillus Rhamnosus-Supplemented Diet On Growth Performance, Immunological Parameters And Protection Against Aeromonas Veronii Challenge In Juvenile Red Tilapias (Oreochromis Spp.), Mariya Sewaka Jan 2018

Synbiotic Effects Of Jerusalem Artichoke (Helianthus Tuberosus) And Lactobacillus Rhamnosus-Supplemented Diet On Growth Performance, Immunological Parameters And Protection Against Aeromonas Veronii Challenge In Juvenile Red Tilapias (Oreochromis Spp.), Mariya Sewaka

Chulalongkorn University Theses and Dissertations (Chula ETD)

Synbiotics, a synergistic combination of probiotics and prebiotics, are now highlighted as one of the most practical nutritional supplements in red tilapia farms. The aim of the present study was to assess the effect of the dietary inclusion of Jerusalem artichoke, Lactobacillus rhamnosus, or their combination, on serum biochemical, growth performance, intestinal morphology, immune parameters, intestinal bacterial count and protection against Aeromonas veronii in juvenile red tilapia (Oreochromis spp.). One hundred and eighty male red tilapias (average body weight of 14.05±0.42 g) were distributed into six 1000-liter tanks containing water under continuous aeration and an average temperature of 25–28°C. Tilapias …

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The Development Of Elisa Kit To Detect Antibody Response Against Porcine Epidemic Diarrhea Virus From Colostrum And Milk, Anchalee Srijangwad Jan 2018

The Development Of Elisa Kit To Detect Antibody Response Against Porcine Epidemic Diarrhea Virus From Colostrum And Milk, Anchalee Srijangwad

Chulalongkorn University Theses and Dissertations (Chula ETD)

Porcine epidemic diarrhea is caused by porcine epidemic diarrhea virus (PEDV), which causes swine watery diarrhea and higher mortality rates in piglets less than one week of age. Outbreaks of PED affect to economic loss, especially in a swine industrial country. Many strategies were used for preventing the outbreak and infection of PEDV, especially maternal antibody stimulation in pigs and then transfer to piglet through colostrum or milk. Thus, the investigation of antibody level for the situation on the farm is required. The method which convenient and rapid than viral neutralization (VN) test is an enzyme-linked immunosorbent assay (ELISA). The …

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Efficacy Of Tetracycline And Enrofloxacin Against Wolbachia Spp. In Microfilaria Of Brugia Pahangi, Pimsiri Piromkij Jan 2018

Efficacy Of Tetracycline And Enrofloxacin Against Wolbachia Spp. In Microfilaria Of Brugia Pahangi, Pimsiri Piromkij

Chulalongkorn University Theses and Dissertations (Chula ETD)

Brugia pahangi is a major causative agent of animal lymphatic filariasis, one of the neclected tropical disease. Recent studies have also indicated that B. pahangi can cause clinical infection in human, with clinical appearances which are consistent with human lymphatic filariasis. Nowadays, the recognition of Wolbachia as new alternative drug choice for treatment and control of lymphatic filariasis is the most important in anti-filarial chemotherapy. Since, Wolbachia play important roles, including the development, viability, fertility and fecundity of filarial parasites. Depletion of Wolbachia results in sterilization, stunting and death of filarial worms. In this study, we investigated the effects of …

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Efficacy And Safety Evaluations Of Topical Proretinal Nanoparticles, Benchaphorn Limcharoen Jan 2018

Efficacy And Safety Evaluations Of Topical Proretinal Nanoparticles, Benchaphorn Limcharoen

Chulalongkorn University Theses and Dissertations (Chula ETD)

Retinal (retinaldehyde) is one of natural vitamin A metabolites. It is widely used in clinical dermatology such as acne, acne scar, photoaging, wrinkles, psoriasis, skin neoplasia and seborrheic dermatitis. Retinal is a pivotal key to regulate keratinocyte proliferation and differentiation in the epidermis resulting in epidermal thickening and inhibit collagen destruction in the dermis. However, topical application of retinal is still irritative to the skin and chemically and photochemically unstable. Topically applied retinal can induce a retinoid dermatitis. This inflammation is induced by an overload of non-physiological amounts of exogenous retinoic acid in the skin. The adverse effects and physicochemical …

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Genome Characterization Of Quinolone Resistance Associated Genes Of Flavobacterium Columnare Isolated From Columnaris Diseased Asian Sea Bass (Lates Calcarif)Er), Putita Chokmangmeepisarn Jan 2018

Genome Characterization Of Quinolone Resistance Associated Genes Of Flavobacterium Columnare Isolated From Columnaris Diseased Asian Sea Bass (Lates Calcarif)Er), Putita Chokmangmeepisarn

Chulalongkorn University Theses and Dissertations (Chula ETD)

Flavobacterium columnare is Gram negative bacteria which caused columnaris disease in various kinds of freshwater fishes. This study is the first report of F. columnare infection in freshwater culturing Asian sea bass in Thailand. Phenotypic characterization, antimicrobial susceptibility test and quinolone resistant-associated genes of F. columnare were performed. A total 15 of F. columnare were isolated from diseased fishes and phylogenetic analysis revealed that these isolates were belonged to genetic group 2 and 4. From disk diffusion test results, 5 and 6 isolates were resistant to oxolinic (OA) and nalidixic acid (NA) respectively. The highest MIC values to OA and …

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Surveillance Of Influenza A Viruses In A Live Bird Market, Yangon, Myanmar During 2017-2018, Khin Thurain Jan 2018

Surveillance Of Influenza A Viruses In A Live Bird Market, Yangon, Myanmar During 2017-2018, Khin Thurain

Chulalongkorn University Theses and Dissertations (Chula ETD)

Live bird markets play a significant role in highly pathogenic avian influenza (HPAI) virus spread and transmission. In Myanmar, the first HPAI-H5N1 outbreak was reported in 2006. Since 2014, routine influenza A virus surveillance in LBMs have been conducted in Myanmar by the Livestock Breeding and Veterinary Department in cooperation with FAO. However, there is limited information on virus characterization and evolution of influenza viruses in Myanmar. The objectives of this thesis were to detect influenza virus subtypes circulating in different poultry species from Mingalar-Taung-Nyunt live bird market (MTN-LBM) and to determine genetic characteristics and genetic diversity of influenza A …

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Appropriate Campylobacter Detection Method And Genetic Variation Of Campylobacter Jejuni In Broilers, Natnicha Phetsri Jan 2018

Appropriate Campylobacter Detection Method And Genetic Variation Of Campylobacter Jejuni In Broilers, Natnicha Phetsri

Chulalongkorn University Theses and Dissertations (Chula ETD)

Campylobacter has been known as an important cause of gastroenteritis in humans. The primary source is raw or undercooked poultry meat. Previous study showed that Campylobacter isolated from slaughterhouses were associated with Campylobacter isolated from broiler farms. Thus, detection of Campylobacter flock status and sorting flocks prior to slaughtering processes may help prevent Campylobacter contamination between Campylobacter-positive and negative flocks during slaughtering processes. Unfortunately, appropriate sample type and isolation method have not been clearly specified for Campylobacter detection at the farm level. Because Campylobacter are fastidious bacteria, improper detection method can attribute to false negative results. Therefore, the objectives of …

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Development Of Reverse Transcription Loop-Mediated Isothermal Amplification Combine With Lateral Flow Device As A Detection Assay For Swine Influenza Virus, Albarriyati Ummi Asih Jan 2018

Development Of Reverse Transcription Loop-Mediated Isothermal Amplification Combine With Lateral Flow Device As A Detection Assay For Swine Influenza Virus, Albarriyati Ummi Asih

Chulalongkorn University Theses and Dissertations (Chula ETD)

Swine influenza causes high morbidity and low mortality in pigs. The disease spreads rapidly in pig farms causing the delay of pig weight to reach market. Moreover, pigs play important role as "mixing vessel" of influenza viruses. Pigs can support infection and replication of influenza viruses from avian, human and swine origins. These lead to major concerns of the important of swine influenza which are not only the economic impacts but also the human health. There is the need of accurate and rapid diagnosis for swine influenza virus (SIV) to minimize further spreading of the viruses in pig population and …

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Pedv Monitoring Using Oral Fluid, Feces And Surface Swab Samples After The Ped Outbreak, Busayamas Jantrasakul Jan 2018

Pedv Monitoring Using Oral Fluid, Feces And Surface Swab Samples After The Ped Outbreak, Busayamas Jantrasakul

Chulalongkorn University Theses and Dissertations (Chula ETD)

A total of 220 pooled samples from 3 collection times were conducted. Eighty pooled samples from the first collection at 1 month after PED outbreak (1MAO) included 10 pooled oral fluid samples, 30 pooled fecal samples and 40 pooled surface swab samples. Eighty-five pooled samples from the second collection at 2MAO included 10 pooled oral fluid samples, 30 pooled fecal samples and 45 pooled surface swab samples. Fifty-five pooled samples from the third collection at 8MAO included 5 pooled oral fluid samples, 15 pooled fecal samples and 35 pooled surface swab samples. All samples collected from the PED-affected swine farm …

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Phenotypic And Genotypic Characteristics Of Extended-Spectrum Β-Lactamase (Esbl) Production And Colistin-Resistance In Salmonella Enterica And Escherichia Coli Isolated From​ Pigs And Their Meat Products In The Border Provinces Between Thailand And Cambodia, Lao Pdr And Myanmar, Kyaw Phyoe Sunn Jan 2018

Phenotypic And Genotypic Characteristics Of Extended-Spectrum Β-Lactamase (Esbl) Production And Colistin-Resistance In Salmonella Enterica And Escherichia Coli Isolated From​ Pigs And Their Meat Products In The Border Provinces Between Thailand And Cambodia, Lao Pdr And Myanmar, Kyaw Phyoe Sunn

Chulalongkorn University Theses and Dissertations (Chula ETD)

A total of 809 samples of pig rectal swab from slaughterhouses (n=441) and pork from retail market (n=368) were collected in the border provinces among Thailand, Cambodia, Lao PDR and Myanmar between October 2016 and March 2017. The objective of this study was to determine resistance to extended-spectrum ß-lactams (ESBLs) and colistin in Salmonella enterica and Escherichia coli. A total of Salmonella (n=463) and E. coli (n=767) were collected and determined for the ESBL-production and for the Minimum Inhibitory Concentrations (MICs) of colistin and; the presence of ESBL gene and mcr gene. The results showed that the prevalence of Salmonella …

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Genetic Characteristics Of Avian Influenza Viruses Isolated From Ducks In Java Island, Indonesia Year 2015-2017, Lestari - Jan 2018

Genetic Characteristics Of Avian Influenza Viruses Isolated From Ducks In Java Island, Indonesia Year 2015-2017, Lestari -

Chulalongkorn University Theses and Dissertations (Chula ETD)

Avian influenza becomes public health concern over years due to the serious impacts in both animal and human health. This study aimed to identify and characterize avian influenza viruses isolated from ducks in Java Indonesia during 2015-2017. Total 50 samples of previously identified as avian influenza viruses were acquired from the virus/culture collections of Disease Investigation Center (DIC) Wates Yogyakarta, Indonesia. The samples were recovered from either the avian influenza surveillance or avian influenza outbreaks in ducks in 3 provinces, East Java (n=26), Central Java (n=16), and Yogyakarta (n=8). Then the samples were processed for influenza A virus screening by …

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Genome Characterization Of Hahella Chejuensis Strain Hn01, A Pathogen Of Red Egg Disease In Tilapia (Oreochromis Sp.), Putu Cri Devischa Gallantiswara Jan 2018

Genome Characterization Of Hahella Chejuensis Strain Hn01, A Pathogen Of Red Egg Disease In Tilapia (Oreochromis Sp.), Putu Cri Devischa Gallantiswara

Chulalongkorn University Theses and Dissertations (Chula ETD)

Red eggs disease is an emerging disease occurred in Tilapia hatcheries which caused by a Gram-negative bacterium called Hahella chejuensis HN01. The disease infects to all the stage of fish eggs and reduces the hatchability. However, there are limited study about the bacterial pathogenicity. In this study, we analyzed the phylogenetic relationship and characterized the virulence traits of Hahella chejuensis by using genomic approach. The bacterial genome was sequenced using Illumina Miseq platform. The phylogenetic tree based on several housekeeping genes confirmed that the bacterium was belong to Genus Hahella and species chejuensis. the Average Nucleotide Identity calculation with the …

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Detection Of Antimicrobial Resistance Determinants In Vibrio Parahaemolyticus Isolated From Cultivated Oysters And Estuarine Waters, Winn Khant Jan 2018

Detection Of Antimicrobial Resistance Determinants In Vibrio Parahaemolyticus Isolated From Cultivated Oysters And Estuarine Waters, Winn Khant

Chulalongkorn University Theses and Dissertations (Chula ETD)

In this study, we aimed to detect antimicrobial resistance determinants and virulence factor genes in V. parahaemolyticus isolates from cultivated oysters and estuarine waters in southern Thailand. A total of 594 V. parahaemolyticus isolates were collected from pooled oysters (n=361) and estuarine waters (n=233). The samples were collected monthly between March 2016 and February 2017 from Thap Put district, Phang Nga province in southern Thailand. Confirmation of V. parahaemolyticus was carried out by PCR assay detecting species-specific (tlh) gene and the presence of virulence genes (tdh and trh). Antimicrobial susceptibility test was performed in eight antimicrobials, and the occurrence of …

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Biomechanical And Biological Properties Of Cryopreserved Canine Amniotic Membrane, Nathawan Withavatpongtorn Jan 2018

Biomechanical And Biological Properties Of Cryopreserved Canine Amniotic Membrane, Nathawan Withavatpongtorn

Chulalongkorn University Theses and Dissertations (Chula ETD)

Amniotic membrane (AM) is the innermost fetal membrane. Due to its many known properties, human AM is a favorable corneal graft. Cryopreservation at -80ºC maintains most of biological properties. Due to shortage of supply, AM of other species are studied. The objective of this study is to explore the biomechanical and biological properties of canine AM (CAM) after cryopreservation for 7 and 30 days. CAMs were collected from healthy bitches undergoing cesarean sections. After preservation, they were tested for tensile strength by Universal Testing Machine, transparency by spectrophotometer, sterility by microbial culture, cell viability by trypan blue staining, and histological …

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The Efficiency Of Pcso-524 On Feline Osteoarthritis Associated With Chronic Kidney Disease, Pemika Dulyapraphant Jan 2018

The Efficiency Of Pcso-524 On Feline Osteoarthritis Associated With Chronic Kidney Disease, Pemika Dulyapraphant

Chulalongkorn University Theses and Dissertations (Chula ETD)

Twenty-six osteoarthritic cats concurrent with chronic kidney disease (CKD) enrolled in this study. All cats were divided randomly into 2 groups; control group (n=12) received placebo and treatment group (n=14) received PCSO-524 for 60 days. Cats had verified stable CKD stage II and had treated and followed up for CKD more than 3 months were included in our study. Before starting the study (day 0), all cats were taken the coxofemoral joint radiographic imaging. Only cats with the radiographic osteoarthritic lesion(s) were recruited. All of them had blood profile check-up, urinalysis, blood pressure measurement, lameness score assessment, acute and chronic …

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