Medicine and Health Sciences Commons^™

Emerging Justifications For Placebic Treatment, Tejas Pulisetty

Quill & Scope

No abstract provided.

Plan B Emergency Contraception: Sampled University Students' Knowledge, Attitudes, And Behaviors, Natalie Hazel

All Graduate Theses, Dissertations, and Other Capstone Projects

The purpose of this research was to assess and evaluate sampled university students' knowledge, attitudes, perceptions, and behaviors related to the Plan B emergency contraceptive. To collect data, a 29 item survey was developed and administered to students in sampled classrooms at Minnesota State University, Mankato campus regarding their knowledge, attitudes, perceptions, and behaviors related to the Plan B emergency contraceptive; as well as demographic information. The findings of the study indicated there were some participants who have much knowledge about the Plan B emergency contraceptive. However, findings also showed that there were a number of participants who lacked knowledge …

Muc1 Regulates Cyclin D1 Gene Expression Through P120 Catenin And Β-Catenin, X. Liu, Thomas C. Caffrey, M. M. Steele, Ashley M. Mohr, Pankaj K. Singh, Prakash Radhakrishnan, David L. Kelly, Y. Wen, Michael A. Hollingsworth

Journal Articles: Eppley Institute

MUC1 interacts with β-catenin and p120 catenin to modulate WNT signaling. We investigated the effect of overexpressing MUC1 on the regulation of cyclin D1, a downstream target for the WNT/β-catenin signaling pathway, in two human pancreatic cancer cell lines, Panc-1 and S2-013. We observed a significant enhancement in the activation of cyclin D1 promoter-reporter activity in poorly differentiated Panc1.MUC1F cells that overexpress recombinant MUC1 relative to Panc-1.NEO cells, which express very low levels of endogenous MUC1. In stark contrast, cyclin D1 promoter activity was not affected in moderately differentiated S2-013.MUC1F cells that overexpressed recombinant MUC1 relative to S2-013.NEO cells that …

Roofer Dies After Gust Of Wind Knocks Him And A Co-Worker Off Roof, Kentucky Injury Prevention And Research Center

Fatality Case Reports--Falls

Around 11:00 a.m. on a windy fall day, a 73-year-old roofer and a roofing coworker arrived at the commercial construction site to roof a newly constructed building. The two roofers climbed an extension ladder to access the roof to determine where they were going to place their anchors to tie off. While reviewing the backside of the commercial structure, a gust of wind around 33 m.p.h. made the two roofers lose their balance and fall from the roof. The 73-year-old roofer landed first and the second roofer landed on top of him. Both men fell 15 feet and 6 inches …

Pediatric Obesity: Taking Advantage Of Well-Child Visits For Early Identification, James E. Gelement

DNP Projects

Background: Pediatric obesity has reached epidemic levels in the United States and the burden of early identification and prevention lies with Primary Care Providers. With this burden comes the responsibility of increased documentation in order for patients to obtain the appropriate counseling, treatment, and referrals.

Objective: The objective of this study is to identify missed opportunities to identify pediatric patients that meet the criteria for overweight or obesity during well-child visits within a primary care practice.

Method: A retrospective chart review of 100 randomly selected patients born between 9/1/2001 and 9/1/2010 that have received a well-child visit between 1/1/2010 and …

Assessment Of The Accuracy Of The Diagnosis For Heart Failure In A Large Metropolitan Health Care System, Sandra Anne Carey

Nursing Dissertations - Archive

Purpose: There has been no significant improvement in heart failure (HF) readmissions regionally or nationally in the United States over the past decade. Additionally, there is no consensus nationally on defining the complex disease state of HF. This study retrospectively describes the accuracy of HF diagnoses in an adult HF patient population in a large metropolitan hospital setting. It also compares readmission rates between patients who were accurately diagnosed for HF versus those who were inaccurately diagnosed.Methods: A retrospective record review (N = 712) was performed at a large metropolitan health care center in North Texas from January 2012 to …

Fathers' Experience With Autism Spectrum Disorder, Linda Sue Frye

Nursing Dissertations - Archive

Autism spectrum disorder (ASD) is a lifelong neurodevelopmental disorder that causes very mild to severe impairments in communication skills, social interactions, and unusual behaviors that interfere with the children's ability to function independently and interact appropriately with other people (American Psychiatric Association [APA], 2013). Children with ASD often appear normal (without physical deformities). The impairments are sources of economic burden, decreased emotional well-being, and decreased family cohesion. Some parents of children with ASD adjust, adapt, and become resilient when facing the multiple challenges of ASD, but others experience anxiety, depression, and poor mental health that can lead to decreased family …

An Exploratory Descriptive Study On Task Shifting, Lori A. Spies

Nursing Dissertations - Archive

Task shifting is an approach to meeting population health needs in developing countries that is encouraged by the WHO and increasingly embraced by Ministries of Health. Task shifting has been implemented formally and informally for years in areas of human resource shortages. While often used, there is a lack of published information available about how best to prepare nurses to take on the expanded role. The nursing intellectual capital (NIC) theory was the theoretical framework for this study. NIC is derived from a business model and is used to represent the manner in which investments in the nurse and healthcare …

Adults With Congenital Heart Disease And Family Planning Decisions, Kathryn A. Osteen

Nursing Dissertations - Archive

Adults with CHD are living longer into adulthood than ever before, which increases the possibility of childbearing. Researchers have focused on the physiology and risks associated with contraception and pregnancy, but miss the personal stories surrounding these decisions. The adult with CHD has concerns about contraception, pregnancy, childbirth, and passing CHD on to offspring. The purpose of this qualitative narrative study is to describe the personal stories of adults with CHD and their family planning decisions.Narrative inquiry, guided by the Roy adaptation model, was chosen for this study because it is appropriate to describe the reproductive decisions of adults with …

Identifying The Characterestics Of Patients With Community Versus Hospital Acquired Pressure Ulcers, Ahmad A. Hamdan

Nursing Dissertations - Archive

Pressure ulcers (PU) are a common problem with important implications for the nursing community and for patients, including pain and suffering, delayed recovery, morbidity, mortality, and healthcare costs, but little is known regarding what characterizes the difference between community acquired pressure ulcers (CAPU) and hospital acquired pressure ulcers (HAPU). Therefore, the purpose of this descriptive correlational retrospective study was to describe patient characteristics associated with CAPU and HAPU. Measured variables included gender, age, ethnicity, PU site, albumin, weight, BMI insurance, length of stay, type of bed, type of admission, and weekend admission, as well as Braden Scale measures of moisture, …

A Comparison Of Instructor Audio-Video With Text-Based Feedback Versus Text-Based Feedback Alone On Students' Perceptions Of Community Of Inquiry Among Rn-To-Bsn Online Students, Marie Kelly Lindley

Nursing Dissertations - Archive

In order to meet the healthcare needs of the US population, Registered Nurses (RNs), with Bachelor of Science in Nursing degrees, are needed. This descriptive posttest study, with independent samples, examined the use of audio-video with text-based feedback versus text-based only feedback on student's perceptions of the community of inquiry RN to BSN online students (n=125), enrolled in one course at one university. The COI survey performed reliably with Cronbach's alpha .94 on the pretest and .98 on the posttest. No statistically significant difference was found between the groups on the pretest for teaching presence (p=.31), social presence (p=.40) or …

Self-Efficacy Of Caregiving And Caregiver Stress In Adult Informal Caregivers Of Individuals At The End-Of-Life, Michelle Mary Hampton

Nursing Dissertations - Archive

Researchers have established an association between the stressors of providing informal care and physiological and psychological health risks (Applebaum & Breitbart, 2013; Au et al., 2010; Bainbridge, Krueger, Lohfeld, & Brazil, 2009; Gilliam & Steffen, 2006; Mausbach et al., 2012; Merluzzi, Philip, Vachon, & Heitzmann, 2011; Perez, Ussher, Butow, & Wain, 2011). Despite the recognized negative consequences of informal care, researchers have identified the existence of mediators which have the potential to buffer, manage, transform, or prevent the negative influence of stress (Bolden & Newsome-Wicks, 2008; Kreitler, Peleg, & Ehrenfeld, 2007; Merluzzi et al., 2011; Roscoe, Corsentino, Watkins, McCall, & …

Factors Affecting Quality Of Life In Persons With Multiple Sclerosis, Brian E. Holland

Nursing Dissertations - Archive

Multiple sclerosis is an autoimmune disease that can negatively affect the physical, emotional, and social aspects of one's life. This purpose of this descriptive-correlational study was to explore the relationships among individual characteristics, perceived healthcare provider engagement, treatment adherence, functional ability, and quality of life in persons with multiple sclerosis (MS). Additionally, differences in perceived healthcare provider engagement, treatment adherence, functional ability, and quality of life were examined across the four MS disease stages of relapsing-remitting, secondary-progressive, primary-progressive, and progressive-relapsing. A convenience sample of individuals (N = 158) with MS completed an online survey related to perceived healthcare provider engagement, …

Improving Provider Adherence With Completion Of A Written Asthma Action Plan According To Evidence-Based Practice Guidelines, Stephanie L. Collins

Doctor of Nursing Practice (DNP) Scholarly Projects - Archive

Asthma is a serious chronic inflammatory health condition of the airways that causes many Americans to experience distress {Agency for Health Care Research and Quality [AHRQ], 2009). Asthma prevalence over the age of 16 has become particularly concerning. It is a costly disease that has increased drastically in recent decades (AHRQ, 2009). Many factors cause our nation to collectively attempt to improve the quality of care for these individuals. The primary goal is to treat the underlying inflammation and manage daily symptoms. Regardless of national recommendations, health care providers experience challenges with compliance. In one study, all primary care providers …

Parent Health Literacy And Communication With Diabetes Educators In A Pediatric Diabetes Clinic: A Mixed Methods Approach, Carol J. Howe

Nursing Dissertations - Archive

The association between low health literacy and poor patient-provider communication has been well established in adult patients. Little pediatric research has been reported on the relationship between parent health literacy and parent-provider communication. This mixed methods study examined and explored how parent health literacy affected the communication process with diabetes educators in a pediatric diabetes clinic. This study used the Conceptual Framework of Provider Patient Communication as a theoretical framework. Health literacy was measured with the Rapid Estimate of Adult Health Literacy in Medicine. Quality of communication was assessed with quantitative and qualitative methods using 5 subscales of the Interpersonal …

The Role Of Positive Alcohol Expectancies In Underage Binge Drinking Among College Students, Nicole M Mcbride, Blake Barrett, Kathleen A. Moore, Lawrence Schonfeld

Mental Health Law & Policy Faculty Publications

Objective: This study explored associations between positive alcohol expectancies, and demographics, as well as academic status and binge drinking among underage college students. Participants: A sample of 1,553 underage college students at 3 public universities and 1 college in the Southeast who completed the Core Alcohol and Drug Survey in the Spring 2013 semester. Methods: A series of bivariate analyses and logistic regression models were used to examine associations between demographic and academic status variables as well as positive alcohol expectancies with self-reported binge drinking. Positive alcohol expectancies were examined in multivariable models via 2 factors derived from principal component …

Genetic Manipulation Of The Toxoplasma Gondii Genome By Fosmid Recombineering, Sumiti Vinayak, Carrie F. Brooks, Anatoli Naumov, Elena Suvorova, Michael W. White, Boris Striepen

Molecular Medicine Faculty Publications

Apicomplexa are obligate intracellular parasites that cause important diseases in humans and animals. Manipulating the pathogen genome is the most direct way to understand the functions of specific genes in parasite development and pathogenesis. In Toxoplasma gondii, nonhomologous recombination is typically highly favored over homologous recombination, a process required for precise gene targeting. Several approaches, including the use of targeting vectors that feature large flanks to drive site-specific recombination, have been developed to overcome this problem. We have generated a new large-insert repository of T. gondii genomic DNA that is arrayed and sequenced and covers 95% of all of the …

Pe-Db: A Database Of Structural Ensembles Of Intrinsically Disordered And Unfolded Proteins, Mihaly Varadi, Simone Kosol, Pierre Lebrun, Erica Valentini, Martin Blackledge, A. Keith Dunker, Isabella C. Felli, Julie D. Forman-Kay, Richard Kriwacki, Roberta Pierattelli, Joel Sussman, Dmitri I. Svergun, Vladimir N. Uversky, Michele Vendruscolo, David Wishart, Peter E. Wright, Peter Tompa

Molecular Medicine Faculty Publications

The goal of pE-DB (http://pedb.vib.be) is to serve as an openly accessible database for the deposition of structural ensembles of intrinsically disordered proteins (IDPs) and of denatured proteins based on nuclear magnetic resonance spectroscopy, small-angle X-ray scattering and other data measured in solution. Owing to the inherent flexibility of IDPs, solution techniques are particularly appropriate for characterizing their biophysical properties, and structural ensembles in agreement with these data provide a convenient tool for describing the underlying conformational sampling. Database entries consist of (i) primary experimental data with descriptions of the acquisition methods and algorithms used for the ensemble …

Identifying Novel Cell Cycle Proteins In Apicomplexa Parasites Through Co-Expression Decision Analysis, Carrie L. Butler, Olivier Lucas, Stefan Wuchty, Bin Xue, Vladimir N. Uversky, Michael White

Molecular Medicine Faculty Publications

No abstract provided.

Intrinsic Disorder In The Bk Channel And Its Interactome, Zhenling Peng, Yoshihisa Sakai, Lukasz Kurgan, Bernd Sokolowski, Vladimir N. Uversky

Molecular Medicine Faculty Publications

The large-conductance Ca2+-activated K+ (BK) channel is broadly expressed in various mammalian cells and tissues such as neurons, skeletal and smooth muscles, exocrine cells, and sensory cells of the inner ear. Previous studies suggest that BK channels are promiscuous binders involved in a multitude of protein-protein interactions. To gain a better understanding of the potential mechanisms underlying BK interactions, we analyzed the abundance, distribution, and potential mechanisms of intrinsic disorder in 27 BK channel variants from mouse cochlea, 104 previously reported BK-associated proteins (BKAPS) from cytoplasmic and membrane/cytoskeletal regions, plus BK β- and γ-subunits. Disorder was evaluated using the MFDp …

Female Rats Are More Susceptible To Central Nervous System Oxygen Toxicity Than Male Rats, Heather E. Held, Raffaele Pilla, Geoffrey E. Ciarlone, Carol S. Landon, Jay B. Dean

Molecular Pharmacology & Physiology Faculty Publications

Tonic–clonic seizures typify central nervous system oxygen toxicity (CNS-OT) in humans and animals exposed to high levels of oxygen, as are encountered during scuba diving. We previously demonstrated that high doses of pseudoephedrine (PSE) decrease the latency to seizure (LS) for CNS-OT in young male rats. This study investigated whether female rats respond similarly to PSE and hyperbaric oxygen (HBO). We implanted 60 virgin stock (VS) and 54 former breeder (FB) female rats with radio-telemetry devices that measured brain electrical activity. One week later, rats were gavaged with saline or PSE in saline (40, 80, 120, 160, or 320 mg/kg) …

Controversial Issues In Kyphoplasty And Vertebroplasty In Osteoporotic Vertebral Fractures, Ioannis D. Papanastassiou, Andreas Filis, Maria A. Gerochristou, Frank D. Vrionis

Orthopaedics and Sports Medicine Faculty Publications

Kyphoplasty (KP) and vertebroplasty (VP) have been successfully employed for many years for the treatment of osteoporotic vertebral fractures. The purpose of this review is to resolve the controversial issues raised by the two randomized trials that claimed no difference between VP and SHAM procedure. In particular we compare nonsurgical management (NSM) and KP and VP, in terms of clinical parameters (pain, disability, quality of life, and new fractures), cost-effectiveness, radiological variables (kyphosis correction and vertebral height restoration), and VP versus KP for cement extravasation and complications profile. Cement types and optimal filling are analyzed and technological innovations are presented. …

Synthesis Of 4-Aryl-6-Indolylpyridine-3-Carbonitriles And Evaluation Of Their Antiproliferative Activity, Naglaa Salem El-Sayed, Amir Nasrolahi Shirazi, Magda Goda El-Meligy, Ahmed Kamel El-Ziaty, Zenat Adeeb Nagib, Keykavous Parang

Pharmacy Faculty Articles and Research

A novel class of 6-indolypyridine-3-carbonitrile derivatives were synthesized and evaluated for antiproliferative activities to establish structure–activity relationship. The synthesis was carried out through one-pot multicomponent reaction of 3-acetylindole, aromatic aldehydes, ethyl cyanoacetate, and ammonium acetate in the presence of piperidine as a catalyst, using a microwave irradiation method or a traditional thermal method. This was followed by chlorination for compounds 13a–e and subsequent nucleophilic substitution of the chlorine group by ethylenediamine at C2 position of the pyridine ring. The antiproliferative activity of these new nicotinonitriles was evaluated against human ovarian adenocarcinoma (SK-OV-3), breast adenocarcinoma (MCF-7), and cervix adenocarcinoma (HeLa) cells. …

Global Analysis Of Chlorella Variabilis Nc64a Mrna Profiles During The Early Phase Of Paramecium Bursaria Chlorella Virus-1 Infection, Janet M. Rowe, Adrien Jeanniard, James R. Gurnon, Yuannan Xia, David D. Dunigan, James L. Van Etten, Guillaume Blanc

Nebraska Center for Virology: Faculty Publications

The PBCV-1/Chlorella variabilis NC64A system is a model for studies on interactions between viruses and algae. Here we present the first global analyses of algal host transcripts during the early stages of infection, prior to virus replication. During the course of the experiment stretching over 1 hour, about a third of the host genes displayed significant changes in normalized mRNA abundance that either increased or decreased compared to uninfected levels. The population of genes with significant transcriptional changes gradually increased until stabilizing at 40 minutes post infection. Functional categories including cytoplasmic ribosomal proteins, jasmonic acid biosynthesis and anaphase promoting …

Dynamic Attachment Of Chlorovirus Pbcv-1 To Chlorella Variabilis, Irina Agarkova, Brigitte Hertel, Xinzheng Zhang, Les Lane, Alexander Tchourbanov, David D. Dunigan, Gerhard Thiel, Michael G. Rossmann, James L. Van Etten

Nebraska Center for Virology: Faculty Publications

Chloroviruses infect their hosts by specifically binding to and degrading the cell wall of their algal hosts at the site of attachment, using an intrinsic digesting enzyme(s). Chlorovirus PBCV-1 stored as a lysate survived longer than virus alone, suggesting virus attachment to cellular debris may be reversible. Ghost cells (algal cells extracted with methanol) were used as a model to study reversibility of PBCV-1 attachment because ghost cells are as susceptible to attachment and wall digestion as are live cells. Reversibility of attachment to ghost cells was examined by releasing attached virions with a cell wall degrading enzyme extract. The …

Genetic Variation And Possible Mechanisms Driving The Evolution Of Worldwide Fig Mosaic Virus Isolates, Jeewan Jyot Walia, Anouk Willemsen, Eminur Elci, Kadriye Caglayan, Bryce W. Falk, Luis Rubio

Nebraska Center for Virology: Faculty Publications

Fig mosaic virus (FMV) is a multipartite negative-sense RNA virus infecting fig trees worldwide. FMV is transmitted by vegetative propagation and grafting of plant materials, and by the eriophyid mite Aceria ficus. In this work, the genetic variation and evolutionary mechanisms shaping FMV populations were characterized. Nucleotide sequences from four genomic regions (each within the genomic RNAs 1, 2, 3, and 4) from FMV isolates from different countries were determined and analyzed. FMV genetic variation was low, as is seen for many other plant viruses. Phylogenetic analysis showed some geographically distant FMV isolates which clustered together, suggesting long-distance migration. …

Risk Factors For Early Childhood Infection Of Human Herpesvirus-8 In Zambian Children: The Role Of Early Childhood Feeding Practices, Kay L. Crabtree, Janet M. Wojcicki, Veenu Minhas, David R. Smith, Chipepo Kankasa, Charles D. Mitchell, Charles Wood

Nebraska Center for Virology: Faculty Publications

Background—Human herpesvirus-8 (HHV-8) infection in early childhood is common throughout sub-Saharan Africa with prevalence increasing throughout childhood. Specific routes of transmission have not been clearly delineated, though HHV-8 is present in high concentrations in saliva.

Methods—To understand the horizontal transmission of HHV-8 within households to children we enrolled for cross-sectional analysis, 251 households including 254 children, age two and under, in Lusaka, Zambia. For all children, plasma was screened for HHV-8 and HIV-1 and health and behavioral questionnaires were completed. Multi-level logistic regression analysis was conducted to assess independent factors for HHV-8 infection in children.

Results—Risk factors for HHV-8 infection …

Turnip Crinkle Virus Coat Protein Inhibits The Basal Immune Response To Virus Invasion In Arabidopsis By Binding To The Nac Transcription Factor Tip, Teresa Donze, Feng Qu, Paul Twigg, T. Jack Morris

Nebraska Center for Virology: Faculty Publications

Turnip crinkle virus (TCV) has been shown to interact with a NAC transcription factor, TIP, of Arabidopsis thaliana, via its coat protein (CP). This interaction correlates with the resistance response manifested in TCV-resistant Arabidopsis ecotype Di-17. We report that failure of a mutated CP to interact with TIP triggered the corresponding TCV mutant (R6A) to cause more severe symptoms in the TCV-susceptible ecotype Col-0. We hypothesized that TCV regulates antiviral basal immunity through TIP-CP interaction. Consistent with this hypothesis, we found that the rate of accumulation of R6A was measurably slower than wild-type TCV over the course of an …

African American Families' Expectations And Intentions For Mental Health Services, Cynthia J. Najdowski, Richard Thompson, Barbara L. Dancy, Tisha R. A. Wiley, Sylvia P. Perry, Jason Wallis, Yara Mekawi, Kathleen Knafl

Psychology Faculty Scholarship

A cross-sectional qualitative descriptive design was used to examine the links among expectations about, experiences with, and intentions toward mental health services. Individual face-to-face interviews were conducted with a purposive sample of 32 African American youth/mothers dyads. Content analysis revealed that positive expectations were linked to positive experiences and intentions, that negative expectations were not consistently linked to negative experiences or intentions, nor were ambivalent expectations linked to ambivalent experiences or intentions. Youth were concerned about privacy breeches and mothers about the harmfulness of psychotropic medication. Addressing these concerns may promote African Americans’ engagement in mental health services.

Research Week 2014, Linda Gardiner

Office of Research Institutional Research and Scholarship

No abstract provided.