Medicine and Health Sciences Commons^™

Prolonged Cyclooxygenase-2 Induction In Neurons And Glia Following Traumatic Brain Injury In The Rat, Kenneth I. Strauss, Mary F. Barbe, Renee Marshall Demarest, Ramesh Raghupathi, Samir Mehta, Raj K. Narayan

Rowan-Virtua School of Osteopathic Medicine Departmental Research

Cyclooxygenase-2 (COX2) is a primary inflammatory mediator that converts arachidonic acid into precursors of vasoactive prostaglandins, producing reactive oxygen species in the process. Under normal conditions COX2 is not detectable, except at low abundance in the brain. This study demonstrates a distinctive pattern of COX2 increases in the brain over time following traumatic brain injury (TBI). Quantitative lysate ribonuclease protection assays indicate acute and sustained increases in COX2 mRNA in two rat models of TBI. In the lateral fluid percussion model, COX2 mRNA is significantly elevated (>twofold, p < 0.05, Dunnett) at 1 day postinjury in the injured cortex and bilaterally in the hippocampus, compared to sham-injured controls. In the lateral cortical impact model (LCI), COX2 mRNA peaks around 6 h postinjury in the ipsilateral cerebral cortex (fivefold induction, p < 0.05, Dunnett) and in the ipsilateral and contralateral hippocampus (two- and six-fold induction, respectively, p < 0.05, Dunnett). Increases are sustained out to 3 days postinjury in the injured cortex in both models. Further analyses use the LCI model to evaluate COX2 induction. Immunoblot analyses confirm increased levels of COX2 protein in the cortex and hippocampus. Profound increases in COX2 protein are observed in the cortex at 1-3 days, that return to sham levels by 7 days postinjury (p < 0.05, Dunnett). The cellular pattern of COX2 induction following TBI has been characterized using immunohistochemistry. COX2-immunoreactivity (-ir) rises acutely (cell numbers and intensity) and remains elevated for several days following TBI. Increases in COX2-ir colocalize with neurons (MAP2-ir) and glia (GFAP-ir). Increases in COX2-ir are observed in cerebral cortex and hippocampus, ipsilateral and contralateral to injury as early as 2 h postinjury. Neurons in the ipsilateral parietal, perirhinal and piriform cortex become intensely COX2-ir from 2 h to at least 3 days postinjury. In agreement with the mRNA and immunoblot results, COX2-ir appears greatest in the contralateral hippocampus. Hippocampal COX2-ir progresses from the pyramidal cell layer of the CA1 and CA2 region at 2 h, to the CA3 pyramidal cells and dentate polymorphic and granule cell layers by 24 h postinjury. These increases are distinct from those observed following inflammatory challenge, and correspond to brain areas previously identified with the neurological and cognitive deficits associated with TBI. While COX2 induction following TBI may result in selective beneficial responses, chronic COX2 production may contribute to free radical mediated cellular damage, vascular dysfunction, and alterations in cellular metabolism. These may cause secondary injuries to the brain that promote neuropathology and worsen behavioral outcome.

Sequential Group Trial To Determine Gastrointestinal Site Of Absorption And Systemic Exposure Of Azathioprine, Jane M. Gervasio, Rex O. Brown, John Lima, M. G. Tabbaa, Thomas Abell, Robert Werkman, Lynda J. Haberer, Lawrence J. Hak

Scholarship and Professional Work – COPHS

Azathioprine (AZA) is used in the treatment of patients with refractory inflammatory bowel disease; however, its use is limited because of systemic toxicity associated with long-term use. Ileocecal delivery of AZA might be advantageous if local intestinal therapeutic effects could be provided with decreased systemic side effects. Decreased cecal systemic absorption would allow higher dosages of AZA to be administered. A two-phase study was performed to compare the systemic exposure of AZA and 6-mercaptopurine (6-MP) following administration of AZA into the stomach, jejunum, and cecum and to compare the systemic exposure to AZA and 6-MP following administration of three different …

Morphological, Molecular, And Biogeographical Variation Within The Imperiled Virginia Spiraea, Constance Anders

Masters Theses & Specialist Projects

Spiraea virginiana Britton is a rare federally listed rhizomatous shrub endemic to the southern Blue Ridge and Appalachian Plateau physiographic provinces. Populations of S. virginiana are found restricted to scoured sections of high gradient streams within the Ohio River drainage. Present evidence indicates the species is reproducing asexually, most probably through the deposition of rhizomes from upstream populations forming new downstream ramets. Phenotypic variation was examined through a morphometric evaluation of 25 leaf measurements and analyzed using Principal Components Analysis (PCA) and discriminant function analysis. Identity and structure at the molecular level as examined with Randomly Amplified Polymorphic DNA (RAPDs) …

Com Outlook August 2000, College Of Osteopathic Medicine

COM Outlook

No abstract provided.

Eye Position Signal Modulates A Human Parietal Pointing Region During Memory-Guided Movements., J F Desouza, S P Dukelow, J S Gati, R S Menon, R A Andersen, T Vilis

Brain and Mind Institute Researchers' Publications

Using functional magnetic resonance imaging, we examined the signal in parietal regions that were selectively activated during delayed pointing to flashed visual targets and determined whether this signal was dependent on the fixation position of the eyes. Delayed pointing activated a bilateral parietal area in the intraparietal sulcus (rIPS), rostral/anterior to areas activated by saccades. During right-hand pointing to centrally located targets, the left rIPS region showed a significant increase in activation when the eye position was rightward compared with leftward. As expected, activation in motor cortex showed no modulation when only eye position changed. During pointing to retinotopically identical …

Children Of Alcoholics: An Update., D Z Lieberman

Psychiatry and Behavioral Sciences Faculty Publications

One of four children in the United States lives in a family in which the child is exposed to alcoholism. Children of alcoholics (COAs) are two to ten times more likely to develop alcoholism than non-COAs. This paper reviews studies that have attempted to identify risk factors that mediate the increased vulnerability and the protective factors that moderate the risk. Factors discussed include parental antisocial personality disorder, externalizing behavior, internalizing symptoms, differential response to the effects of alcohol, and positive and negative alcohol-related expectancies. The heterogeneous nature of COAs is emphasized, and some of the challenges related to treatment and …

Regional Variation In Recruitment Of Hemlock Seedlings And Saplings In The Upper Great Lakes, Usa, Thomas P. Rooney, Ronald J. Mccormick, Stephen L. Solheim, Donald M. Waller

Biological Sciences Faculty Publications

Mature eastern hemlock–northern hardwood forest cover decreased drastically in the upper Midwest following European settlement and has yet to rebound substantially. Previous studies show that stands retaining substantial hemlock canopy coverage have low hemlock seedling and sapling densities. Results from various geographically restricted studies suggest several possible mechanisms that could cause low seedling or sapling density. We examined the relative importance of these proposed mechanisms in the Southern Superior Uplands Section of the Laurentian Forest Province. We surveyed 294- m2 plots in 100 hemlock stands in northern Wisconsin and western upper Michigan to assess how these proposed mechanisms affect the …

Peer Support As A Protective Factor In The Impact Of War On Adolescents In Lebanon, Nadine S. Fakhran

All-Inclusive List of Electronic Theses and Dissertations

Children exposed to situations of war have been shown to develop behavioral as well as psychological difficulties. Researchers have observed, however, that not all children manifest these difficulties in reaction to war situations. Therefore, scientists have attempted to look at resilient children, investigating protective and vulnerability factors in the child's environment. One such protective factor is social support. Although there have been several studies on social support, those studies have tended to focus on family support. There seems to be a void in the literature on the effects of peer support on post-traumatic stress reactions in children. The present study …

Selection Of Burkholderia Pseudomallei Antigens For Antibody Detection By Indirect Hemagglutination Method, W. Chatwiriyacharoen

Chulalongkorn Medical Journal

No abstract provided.

Continuing Medical Education (Cme) In The Near Future, S. Chittmittrapap

Chulalongkorn Medical Journal

No abstract provided.

The Clinical Study Of Efficacy And Safety Of Tamsulosin Hydrochloride For The Treatment Of Symptomatic Benigh Prostatic Hypertrophy In Thai Men, A. Kongkanand, K. Ratana-Olarn, A. Tantiwong, S. Ruangdilokrat, Y. Yongslertvidhya, N. Kunachaichot, S. Leungwattanakij

Chulalongkorn Medical Journal

No abstract provided.

A Comparative Study Of Basic Manual Hematology Laboratoryskill Examination Scores Among 4 Programs Medicalstudents Of The Faculty Of Medicine Ofchulalongkorn University, A. Siritantikorn, V. Wiwanitkit

Chulalongkorn Medical Journal

No abstract provided.

Tobacco Consumption: Policy And Control, V. Lohsoonthorn

Chulalongkorn Medical Journal

No abstract provided.

Children's Understanding Of The Concept Of Physical Activity, Stewart G. Trost, Angela M. Morgan, Ruth P. Saunders, Gwen A. Felton, Dianne S. Ward, Russell R. Pate

Faculty Publications

This study evaluated 4th-grade students' understanding of the concept of physical activity and assessed the effects of two interventions to enhance the students' understanding of this concept. Students were randomly assigned to 1 of 3 conditions: the video group (n=40) watched a 5-min video describing physical activity; the verbal group (n=42) listened to a generic description of physical activity; the control group received no instruction (n=45). Students completed a 17-item checklist testing their understanding of the concept of physical activity. Compared to controls, students in the verbal and video group demonstrated significantly higher checklist …

Bipolar Disorder And Locus Of Control: Implications For Treatment Compliance, Linda J. Ryan-Cox

Theses

Theoretically, a paradigm shift in the 1960s made community-based care and out-patient treatment an acceptable alternative to institutionalization of the chronically mentally ill. Utilizing the Levenson Multidimensional Locus of Control Scale for Psychiatric Patients, the author conducted a causal-comparative study to determine if a link exists between having bipolar disorder, a chronic mental illness, and an external locus of control, a link which might explain the inability of this population to maintain their optimal level of mental health utilizing the out-patient treatment model. Though the study failed to demonstrate any significant differences in external measures of loci of control between …

Genitourinary Tuberculosis: A Profile Of 55 In-Patients, N P. Buchholz, S Salahuddin, R Haque

Section of Urology

Objective: To outline the pattern and trends in major cases of genito-urinary tuberculosis (GUTB) which require hospital treatment.
Method: We retrospectively reviewed 55 patients with proven GUTB who were treated as in-patients in a major referral hospital in Pakistan.
Results: The male/female ratio was 3:1. Prevailing symptoms were lower urinary tract symptoms, flank pain, gross hematuria and fever. A urine culture was positive for tuberculosis (TB) in 57%, bladder biopsies in 54%. For renal TB, intravenous urogram (IVU) and ultrasound were suggestive in about 50% of cases. Ultrasound was very helpful in the diagnosis of testicular TB. Patients underwent surgery …

Comparison Of Health Promotion Behaviors And Performance On The Navy Physical Readiness Test Among Active Duty Shipboard Personnel With And Without Anterior Knee Pain, Robin Theresa Mckenzie Phd, Msn, Rn

Dissertations

Health promotion is the number one health priority of the nation, yet, health promoting behaviors (HPB) are rarely part of the management plan for persons with anterior knee pain (AKP). In an active duty military population, AKP can interfere with performance on the required, semi-annual physical readiness test (PRT). The purpose of this descriptive, comparative study was to compare the relationships among HPB, AKP and performance on the PRT between two groups of active duty Navy shipboard personnel (those with AKP and those without AKP). Pender's (1996) Health Promotion Model (HPM) served as the theoretical framework. Subjects completed demographic and …

A Comparison Of The Effectiveness Of Eye Protection Methods Used In General Anesthesia, Larry W. Johnson

Theses and Dissertations

The purpose of this quasi experimental, clinical study was to determine whether taping of the eye or applying ophthalmic ointment along with taping of the eye yields a difference in occurrence and symptoms of corneal epithelial defects in surgical patients undergoing general anesthesia. Orem's Self-Care Deficit Theory provided the theoretical framework for this study.

Fifty subjects were randomly selected from a convenience sample of surgical patients in two 300 bed hospitals located in a Midwestern state. The subjects ranged from 18 to 60 years of age. The subjects had no history of previous eye surgery or glaucoma, and were not …

Comparison Of The Cost Of Ectopic Pregnancymanagement : Surgery Versus Methotrexate, R. Aeng, P. Wongprachoom

Chulalongkorn Medical Journal

No abstract provided.

Peripheral Arterial Disease In Diabetes Mellitusat Banpong Hospital, K. Jintanon, B. Dethdit

Chulalongkorn Medical Journal

No abstract provided.

Comparative Study Of Efficacy Of Cefotaxime Sodium Versusampicillin Plus Gentamicin For The Treatmentof Postcesarean Endomyometritis, P. Surichamorn

Chulalongkorn Medical Journal

No abstract provided.

Eosinophilic Granuloma Presentingas Periorbital Cellulitis, P. Hirunwiwatkul, W. Tulvatana, N. Kasetsuwan, I. Nuchprayoon

Chulalongkorn Medical Journal

No abstract provided.

Vibrio Cholerae H-Ns Silences Virulence Gene Expression At Multiple Steps In The Toxr Regulatory Cascade, Melinda B. Nye, James D. Pfau, Karen Skorupski, Ronald K. Taylor

Dartmouth Scholarship

H-NS is an abundant nucleoid-associated protein involved in the maintenance of chromosomal architecture in bacteria. H-NS also has a role in silencing the expression of a variety of environmentally regulated genes during growth under nonpermissive conditions. In this study we demonstrate a role for H-NS in the negative modulation of expression of several genes within the ToxR virulence regulon ofVibrio cholerae. Deletion of hns resulted in high, nearly constitutive levels of expression of the genes encoding cholera toxin, toxin-coregulated pilus, and the ToxT virulence gene regulatory protein. For the cholera toxin- and ToxT-encoding genes, elevated expression in an …

A New Role For A Snare Protein As A Regulator Of The Ypt7/Rab-Dependent Stage Of Docking, Christian Ungermann, Albert Price, William Wickner

Dartmouth Scholarship

The homotypic fusion of yeast vacuoles occurs in an ordered cascade of priming, docking, and fusion. The linkage between these steps has so far remained unclear. We now report that Vam7p (the vacuolar SNAP-23/25 homolog) signals from the cis-SNARE complex to Ypt7p (the vacuolar Rab/Ypt) to initiate the docking process. After Vam7p has been released from the cis-SNARE complex by Sec18p-mediated priming, it is still required for Ypt7p-dependent docking and it needs Ypt7p to remain on the vacuole.

La Notion De Danger, Jean M. Kanellopoulos, David M. Ojcius

All Dugoni School of Dentistry Faculty Articles

Pendant de nombreuses années, les recherches en immunologie ont été centrées sur la réponse immunitaire adaptative, et la plupart des immunologistes considéraient que sa fonction principale était de faire la distinction entre soi et non soi. Plus récemment, plusieurs équipes ont proposé que la fonction principale du système immunitaire est en réalité d’éliminer les agents pathogènes. Or, c’est le système immunitaire inné, longtemps négligé, qui joue un rôle essentiel dans l’induction d’une réponse contre les microorganismes. Ce système réagit précocement aux infections, voire instantanément, puis il induit et oriente la réponse du système adaptatif. Plusieursthéories ont été élaborées pour expliquer …

Free Radical Production In Hypoxic Pulmonary Artery Smooth Muscle Cells, David W. Killilea, Raymond Hester, Ron Balczon, Pavel Babal, Mark N. Gillespie

University Faculty and Staff Publications

This study used an inexpensive and versatile environmental exposure system to test the hypothesis that hypoxia promoted free radical production in primary cultures of rat main pulmonary artery smooth muscle cells (PASMCs). Production of reactive species was detected by fluorescence microscopy with the probe 2', 7'-dichlorodihydrofluorescein, which is converted to the fluorescent dichlorofluorescein (DCF) in the presence of various oxidants. Flushing the airspace above the PASMC cultures with normoxic gas (20% O(2), 75% N(2), and 5% CO(2)) resulted in stable PO(2) values of approximately 150 Torr, whereas perfusion of the airspace with hypoxic gas (0% O(2), 95% N(2), and 5% …

Characterization Of 50s Ribosomal Subunit Assembly Inhibition In Erythromycin Treated Escherichia Coli Cells., Jerry Edward Usary

Electronic Theses and Dissertations

Erythromycin has long been recognized for its ability to inhibit protein synthesis by interfering with mRNA translation on the bacterial ribosome. We have recently shown that erythromycin also inhibits the assembly of the 50S ribosomal subunit in growing bacterial cells. The nature of this assembly inhibition has been investigated using ³H-uridine pulse-chase labeling of control and erythromycin treated E. coli cells.

Subunit assembly was examined by sucrose gradient centrifugation of labeled cell lysates. Normal assembly kinetics of subunit assembly were observed in control cells at 37°C. Formation of the 30S subunit was completed by 7.5 minutes and assembly of …

Gender Identity Development In Self-Disclosed Gay Men In Hidalgo County, Texas, Sammy Lee Flanagan Jr.

Theses and Dissertations - UTB/UTPA

Although gay individuals are now more visible than ever, homosexuality remains among the most stigmatized of personal identities. It is because of the various variables associated with coming out and being out and the multitude of persons affected by an individuals' decision to disclose his orientation that are the focus of this research.

This research is designed to document the “coming out” experiences of self disclosed homosexual men, in Hidalgo County, Texas a predominately Mexican American, Catholic community, This research will look at the identity change that takes place when people choose to disclose their homosexual identity to other people. …

Differential Infectivity And Division Of Toxoplasma Gondii In Human Peripheral Blood Leukocytes, Jacqueline Y. Channon, Rosanne M. Seguin, Lloyd H. Kasper

Dartmouth Scholarship

When tachyzoites were incubated with human peripheral blood leukocytes in vitro, more monocytes and dendritic cells than neutrophils or lymphocytes were infected. Although tachyzoites were able to divide in each of these cell types, monocytes and dendritic cells were more permissive to rapid tachyzoite division than neutrophils or lymphocytes.

Prognostic Impact Of P53 Status In Ewing Sarcoma, E. De Alava, C. Antonescu, A. Panizo, Denis H. Y. Leung, P. Meyers, A. Huvos, F. J. Pardo-Mindan, J. Healey, M. Ladanyi

Research Collection School Of Economics

Disease stage at the time of diagnosis and response to therapy are the main prognostic factors for patients with Ewing sarcoma or peripheral neuroectodermal tumor (ES/PNET). The primary genetic alteration in ES/PNET, the fusion of the EWS gene with FLI1 or ERG, is diagnostically highly specific for these tumors, and molecular variation in the structure of the EWS-FLI1 fusion gene also is of prognostic significance. In contrast, secondary genetic alterations, such as P53 alterations, are relatively uncommon in ES/PNET, and their prognostic impact has not been extensively studied. METHODS: Prechemotherapy, paraffin embedded, nondecalcified, primary tumor material in a well-characterized series …