Medicine and Health Sciences Commons^™

Identification Of Novel Allosteric Regulators Of Human Erythrocyte Pyruvate Kinase, Shilpa S. Kharalkar

Theses and Dissertations

Erythrocyte pyruvate kinase (R-PK) is a key glycolytic enzyme catalyzing the transphosphorylation of phosphoenolpyruvate (PEP) and ADP to pyruvate and ATP respectively3,4. The substrate PEP and product pyruvate of this reaction are involved in a number of energetic and biosynthetic pathways; hence a tight regulation of R-PK activity is crucial not only for glycolysis, but also for the entire cellular metabolism. Deficiency of R-PK is one of the most common enzymatic defects of RBC, and may be caused by mutations of the PK-LR (pyruvate kinase liver red blood cell) gene31, 32. Clinically, R-PK deficiency manifests itself as a chronic life-long …

Influence Of Anti-Cd44 On Murine B Cell Activation, Tiana L. Wyant

Theses and Dissertations

Lymphocyte activation and trafficking are indispensable to the immune system. CD44, an adhesion molecule, plays important roles in T cell activation, lymphocyte homing/trafficking, and tumor metastasis. Although the functions of CD44 have been shown in T cells and other leukocytes, little is known about its role in B cells. The effects of CD44 cross-linking on murine B cell activation via CD40L/IL-4 was explored using the anti-CD44 mAbs RK3G9 and IM7. Immobilized RK3G9 and IM7 could strongly inhibit B cell proliferation and Ig production, with IgE inhibition being prominent. Soluble anti-CD44 had no effect. The inhibitory effect of RK3G9 was not …

Characterization And Functional Analysis Of A Newly Identified Human Mt5-Mmp Transcript Variant Isolated From Multipotent Nt2 Cells, Heather Hamilton Ross

Theses and Dissertations

Membrane-type 5 matrix metalloproteinase (MT5-MMP) is unique among MMP family members as it is predominately expressed in the CNS. Its expression is ubiquitous during brain development and restricted to regions of neurogenesis and neuroplasticity in the adult. MT5-MMP is a mediator of pericellular proteolysis and is thought to have a functional impact on neurite outgrowth. The studies presented in this work were designed to examine MT5-MMP expression in cultured NT2 cells, a model of newogenesis and neuronal differentiation, and in adult neurogenic brain regions. We further sought to overexpress MT5-MMP and test the hypothesis that it plays a role in …

An Evaluation Of Hospital Capital Investment After The Balanced Budget Act, Tae Hyun Kim

Theses and Dissertations

Capital investments in the latest medical equipment and the replacement of aging facilities are important hospital decisions because they may have a significant influence on operating efficiencies and quality of care. However, hospitals experienced a minimal growth rate in capital expenditures which contributed to the aging of the hospital industry's asset base during the late 1990's and early 2000's. One of the underlying reasons behind this lack of growth might be the financial stresses that hospitals were facing after the Balanced Budget Act (BBA) of 1997, which significantly reducedMedicare reimbursement and had an adverse impact on the financial viability of …

Developmental Expression Of Calcium-Binding Proteins In The Avcn And Mntb Of Normal Hearing And Congenitally Deaf Mice, John L. Roebel

Browse all Theses and Dissertations

This experiment analyzes synaptic differences in the central auditory pathway between normal hearing and congenitally deaf (dn/dn) mice, and provides valuable insight into central changes that correspond with human congenital deafness. Specifically, this experiment analyzes developmental expression of the Calcium (Ca2+)-binding proteins Calretinin (CR), Calbindin D-28k (CB) and Parvalbumin (PV) in large excitatory synapses in the anteroventral cochlear nucleus (AVCN) and the medial nucleus of the trapezoid body (MNTB) of normal and dn/dn mice. Immunofluorescence imaging with primary antibodies detecting CR, CB or PV was used to analyze the expression of each at 9 days, 13 days, 20 days, 30 …

Clinical Presentation Of Acute Coronary Syndrome: Does Age Make A Difference? Implications For Emergency Nursing, Iesiah M. Harris

Browse all Theses and Dissertations

Accurately recognizing symptoms of Acute Coronary Syndrome (ACS) presents a challenge to Emergency Department (ED) nurses and physicians. Due to the variety of clinical presentations in patients, ACS is frequently missed diagnosed. Studies demonstrate that many patients present with atypical symptoms and presentation varies based on gender. There is, however, a paucity of studies on the presentation of ACS in the elderly. With the older American population growing at exponential rates, it is imperative that studies are conducted to better comprehend ACS clinical presentation in the elderly. By 2020, it is anticipated, that 20% of the population will be 65 …

The Relationship Between Lactic Acid, Reactive Oxygen Species And The Hypoxia-Induced Acidification Seen In Chemosensitive Neurons Of The Nucleus Tractus Solitarius (Nts), Trevor Downing

Browse all Theses and Dissertations

The NTS is one of many sites of chemoreception meaning that it responds to changes in CO2 and pH. NTS neurons also acidify approximately 0.13 pH units in response to hypoxia. Experiments involved brainstem slices exposed to a control hypoxic bout followed by hypoxia in conjunction with different drugs. The drugs included fluorocitrate, 4-hydroxycinnamate, oxygen-glucose deprivation and iodoacetate. Iodoacetate produced the strongest blunting of the hypoxia-induced acidification and decreased the response by ~53%. Results from the studies using the superoxide probe DHE showed an increase in the levels of ROS during the hypoxic exposure prior to reoxygenation. Two ROS scavengers …

Development Of An Audio Visual Tool For Medical Training At Kennedy Space Center, Rimawi, Nidal El

Browse all Theses and Dissertations

As part of an effort to improve efficiency of space-flight medical support at Kennedy Space Center, a training video was created to replace a series of lectures given before a launch or landing of the Space Shuttle. The video was designed to familiarize volunteer physicians from around the country with the specific emergency response protocols for a Space Shuttle launch or landing emergency at Kennedy Space Center. The methods used were consistent with standard film making techniques as outlined in several film making texts. The Production was divided into three phases; A pre-production phase wherein the research, screenwriting and production …

Effect Of The Use Of Amaranth Leaf Flour In The Development Of A Diet For Guinea Pigs, Verónica Del Rocío Guerrero Carrera

Theses and Dissertations

This study was carried out in the San Clemente community. The objective was to analyze the effect of using amaranth leaf flour in the creation of a diet for guinea pigs. The field work was done over a period of 12 months and was done in two phases: Development of the diet: this was done by growing the amaranth, cutting it, and dehydrating it until flour was obtained. Then, it was mixed into the diet in accordance with the pre-determined formula along with the other respective materials. Handling of guinea pigs: This was done in the shed of the San …

Eliciting Facial Expressions In Children With And Without Down Syndrome, Orietta Coz

Master's Theses

This study investigated facial expressions of children between the ages of 10 and 15 years with Down Syndrome (experimental group) and compared them to typically developing children (control group). Elicitation of facial expressions was carried out through showings of video clips. Trained observers were used to code expressions of happiness, anger, and disgust from video recordings that were made of the children’s reactions while they watched the video clips.

I hypothesized that Down Syndrome children will not differ from typically developing children in the frequency of elicited happy expressions. However, I expected them to differ in the frequency of elicited …

Self Reported Pain Diary For Assessment Of Chronic Pain In The Communicative, Cognitively Intact Nursing Home Resident, Kathy K. Hager

DNP Projects

This paper describes a clinical project using a self-reported pain diary for the assessment of chronic pain in the communicative, cognitively-intact nursing home resident. There are estimates of chronic pain among nursing home residents that range from 4 – 83%. The goal of this clinical project was to evaluate a chronic pain diary in relation to its effectiveness and efficiency in accurately assessing pain among nursing home residents. Assessment has been identified as a major barrier in the control of chronic pain, and the self-report is accepted as the most reliable measure of a person’s pain. The self-reported pain diary’s …

A Comparison Of Nurses' Clinical Judgement And The Short Form-Mini Nutritional Assessment Tool In Assessing The Nutritional Status Of Older Patients In An Acute Clinical Setting, Roger Shreeve

Theses : Honours

Decreased nutritional status of older hospitalised patients has been identified as a significant problem. The prevalence of malnutrition in older people has been reported to be between 17% and 65% in acute hospital settings. This has led to the recognition of a need for a valid instrument to adequately assess nutritional status. Tools developed to date still require much time and expertise to implement, limiting their use within the practical setting. Nurses are in an ideal position to conduct nutritional assessments. They possess clinical knowledge which can be utilised to provide individualised and timely assessment. To. date, the literature does …

Role Identity Formation Of Occupational Therapy Students, Denise A. Rotert

Electronic Theses and Dissertations

This study is an examination of the professional socialization process of occupational therapy students from a role identity theoretical perspective. First-year students, second-year students, fieldwork students, and faculty at an occupational therapy educational program at a Midwestern institution volunteered to participate in the study. Data were collected through focus group interviews and surveys. The focus of the study was to determine factors associated with occupational therapy students’ identity salience, commitment, and role-person merger. Interviews and surveys were used to determine what factors, over and above didactic education, influence the socialization of students into the profession of occupational therapy and what …

Low Back Pain In Police Officers, Jennifer Gwyn Arts

Masters Theses

Low back pain (LBP) is something that police officers deal with on a daily basis. The purpose of this research was to explore whether police officers have a higher incidence of LBP than the general population and to explore what factors officers believe contribute to the LBP they experience. Surveys were made available to all police departments in Kent County and all sworn officers were asked to participate. The difference between LBP experienced by police officers (60.2%) and that experienced by the general population (60-90%) was not found to be significant. The top two answers given by officers as to …

Small For Gestational Age Infants: Survival And Impact Of Advanced Maternal Age., Sibylle Kristensen

All ETDs from UAB

No abstract provided.

Cervical Cancer Screening For Hiv-Infected Women In Zambia., Vikrant Sahasrabuddhe

All ETDs from UAB

No abstract provided.

Questionnaires Based On Social Cognitive Theory Constructs Do Not Predict Exercise Adherence In Older Adults After Discharge From Home Health Physical Therapy., Jason Hardage

All ETDs from UAB

No abstract provided.

Antenatal Syphilis Management In Sub -Saharan Africa: Public Health Management Challenges And The Impact Of New Hiv Programs In Zambia., Dara G. Potter

All ETDs from UAB

No abstract provided.

Pediatric Iron Toxicity: The Impact Of Unit Dose Packaging., Ann P. Slattery

All ETDs from UAB

No abstract provided.

Multiple-Locus Variable-Number Tandem-Repeat Analysis (Mlva) For Clonal Characterization Of Methicillin Resistant Staphylococcus Aureus Strains, Matthew Box

All ETDs from UAB

Methicillin resistant Staphylococcus aureus (MRSA) strains have been observed to be associated with community based individuals (CA-MRSA) and hospitalized patients (HA-MRSA). The CA-MRSA and HA-MRSA are no longer distinguishable based on the location of patient when the isolate was first obtained from the patient. These strains are now observed both in the hospital and community settings. While the origin of the isolate does not appear to be critical, it is important to genetically define these isolates to assist in epidemiological investigations and evaluation of infection control measures effectiveness. The conventional method for analyzing this genetic information has been pulsed-field gel …

Micro-Computed Tomographic Analysis Of Bone Healing Subsequent To Graft Placement, Preeti M. Chopra

All ETDs from UAB

A MicroCT analysis of bone healing subsequent to graft (tri calcium phosphate/TCP) placement in the maxillary sinus prior to dental endosteal implant placement was the objective of the current study. Ten trephined rod shaped human bone cores were obtained three months after the placement of particulate graft material and the samples were stored immediately in 10% neutral buffered formalin. Using a Microcomputed tomography, the samples were evaluated at resolutions of 6 and 20μm. The thresholds for bone and graft material were determined using visual image, intensity (grey level) and histogram analyses. The graft and bone threshold was 235 greylevel and …

Underlying Purinergic Signaling Important For Monociliumdependent Signaling In Ductal Epithelia: Implications For Polycystic Kidney Disease, Michael Hovater

All ETDs from UAB

This thesis concerns purinergic signaling in renal epithelial cells of normal and polycystic kidneys. The first section discusses first principles of “purinergic signaling” as they relate to the nephron and the urinary bladder. Remodeled and encapsulated cysts in autosomal dominant PKD (ADPKD) and remodeled “pseudocysts” in autosomal recessive polycystic kidney disease (ARPKD) of the renal collecting duct create an ideal microenvironment for purinergic signaling. Once “trapped” in these microenvironments in a “closed system,” purinergic signaling becomes chronic and plays a significant epigenetic and detrimental role in the progression of ADPKD in particular, once the remodeling of the renal tissue has …

Modulation Of Cell Signaling By Tomoregulins In Embryogenesis And Cancer, Paul William Harms

All ETDs from UAB

Growth factor signals often regulate similar cellular processes both during embryogenesis and in adult homeostasis. Stringent control of these signals ensures proper embryonic development and correct cell physiology in mature individuals. Recently, a family of two members of Tomoregulin (or transmembrane protein with epidermal growth factor-like and two follistatin domains [TMEFF]) was found to interact with transforming growth factor-β (TGF-β) and ErbB signaling pathways, both of which are implicated in development and in cancer biology in the adult. The function as well as the mechanisms of TMEFFs in modulating these two signals has not been elucidated in detail. In this …

A Survey Assessment Of Veterinarians To Determine The Level Of Preparedness For An Infectious Disease Outbreak, Tamara Crutchley Bushell

All ETDs from UAB

Safeguarding animal health is of paramount importance to the US economy, public health and food safety. One of the most important causes of contamination in food products and of food-borne disease in humans is microorganisms that reside in the intestines of animals, such as Salmonella species and Escherichia coli O157:H7. A fundamental knowledge of the epidemiology of diseases, including an understanding of the agents and hosts, is a critical component of early (front-line) detection and prevention of contamination by microorganisms. Front-line detection is provided by animal handlers and personnel working with animals on a day-to-day basis, such as veterinarians. Currently …

Differences In Older Adults' Motivation To Exercise Between Healthy And Cardiac Rehabilitation Populations, Andrew French

Masters Theses

Many positive things come along with aging. The decrease in motivation to exercise is not one of them. It has been shown that as people age their desire to exercise goes down. A major concern with this is that inactivity can lead to serious health problems. The most important of these are cardiac related due to the high possibility of death that accompanies them. Therefore, it is critical to find out what motivates older adults to engage in physical activity in order to decrease the likeliness of cardiac events, and also increase the possibility of secondary prevention by increasing the …

Assessing Personality Traits Of Athletic Trainers At Accredited Universities Associated With The Ohio Valley Conference, Dana Patrick Jergens

Masters Theses

The discipline of athletic training is one which deals with the prevention and care of athletic injuries. Athletic training is a competitive field that is oriented towards athletes and sports medicine caregivers. Certain types of personalities mold athletic trainers and the types of lifestyles necessary to do this line of work. To better determine what types of prospective athletic trainers enter this field, it is interesting to find out if they fit certain personality characteristics. The purpose of this study was to examine the personality traits of athletic trainers from accredited universities affiliated with the Ohio Valley Conference. A total …

The Relationship Between Coronary Calcium And Traditional Cardiac Risk Factors In Men And Women With No Known Cardiac Disease, Tasha Thursby

Masters Theses

The purpose of this study was to determine the relationship between the coronary calcium score and traditional cardiac risk factors in men and women with no known cardiac disease. A second purpose was to determine if there were gender differences in the risk factors that best predict high coronary calcium scores. It was hypothesized that there would be a positive relationship between the coronary calcium score and traditional cardiac risk factors in men and women with no known cardiac disease. It was also hypothesized that there would be gender differences in the risk factors that best predict high coronary calcium …

Comparing Discourse Performance According To Elicitation Procedure, Aimee Wheat

Masters Theses

Four adolescents with closed head injury (CHI) and four matched controls provided speaking and writing samples using the following four elicitation procedures: pictured activity description, story retelling, procedural narrative, and personal narrative. The purpose of the current study was to compare discourse performance according to various elicitation tasks for adolescent with CHI and matched controls using measures of productivity, efficiency, and coherence. Additionally, the relationship between cognitive skills and discourse performance was assessed.

Results indicated that there was a significant difference between the participants with CHI and controls for oral productivity during the story retelling task. During this task, the …

Voice Onset Time Production In Individuals Wth Alzheimer's Disease, Julie M. Baker

Electronic Theses and Dissertations

In the present study, voice onset time (VOT) measurements were compared between a group of individuals with moderate Alzheimer's disease (AD) and a group of healthy age- and gender-matched peers. Participants read a list of consonant-vowel-consonant (CVC) words, which included the six stop consonants. Recordings were gathered and digitized. The VOT measurements were made from oscillographic displays obtained from the Brown Laboratory Interactive Speech System (BLISS) implemented on an IBM-compatible computer. VOT measures for the participants' six stop consonant productions were subjected to statistical analysis. The results of the study indicated that differences in VOT values were not statistically significant …

Articulation And Phonological Systems Of Spanish-English Speaking Florida 4 And 5 Year Olds, Jennifer Dennis

Electronic Theses and Dissertations

There are currently five million children three to five years of age (i.e., 59.5% of all children from three to five), who are bilingual, living in the United States. By 2025, Census data show that the population of Florida will increase by some 26 percent adding another 5.5 million people to the state. There are a limited amount of studies yielding data on the development of Spanish phonology in 4 and 5 year old children residing in the United States, particularly in Florida. Consequently, there is limited normative information pertaining to articulation and phonological development in Spanish speakers. It was …