Medicine and Health Sciences Commons^™

Police In Peril: Determining The Work-Induced Stressors Affecting Officers And The Subsequent Use Of Individualized Coping Strategies, Ami Möller

All-Inclusive List of Electronic Theses and Dissertations

Identifying what stressors affect an officer and how he or she copes is essential in maintaining psychologically healthy officers. Studies have found that police officers consider the organizationally based aspects of the job to greatly influence their level of stress. The present research used the Police Stress Survey and COPE Inventory to identify common stressors and coping strategies among forty-five officers in the state of Indiana. The study attempted to determine whether or not a chronic overall stress -produced by frequent organizational stressors existed, and if this stress led to an increased use of maladaptive coping strategies. Results indicated a …

Effects Of Knee Bracing On Vertical Jumping Performance In Healthy College Aged Males, Rebecca E. Rausch

All-Inclusive List of Electronic Theses and Dissertations

Knee bracing is used at all levels of athletics to prevent or support existing knee injuries; however, little research has been conducted on the effects of knee braces on athletic performance. Therefore, the purpose of this study was to examine how two different types of knee braces, semi-customizable braces and knee sleeves, affect vertical jumping performance. Subjects were nine college aged males who regularly participated in athletic activity. Subjects performed a task consisting of a drop jump from a height of 18 inches, landing in a squat position, followed immediately by a maximal vertical jump while wearing two different knee …

Effects Of An Angled Starting Block On Sprint Start Kinematics, Nathaniel A. Brown

All-Inclusive List of Electronic Theses and Dissertations

The purpose of the present study was to investigate the effects of an angled proto-type starting block on sprint start kinematics. The angled starting block rotates the foot medially at a 16 degree angle, which emulates the 16 degree angle of the ankle on the medial axis of the foot. This 16 degree should allow for the athlete to apply more force against the block, resulting in a higher velocity at take-off and a faster block clearance time. In the present study, six female collegiate sprinters were filmed and analyzed using the angled starting block and a standard starting block. …

The Long-Term Teratogenic Effect Of Prenatal Alcohol Exposure On The Somatosensory And Motor Cortex Of Rats, Tyson Chappell

Theses and Dissertations (ETD)

The primary goal of this dissertation was to examine the long-term effect of prenatal alcohol exposure (PAE) on the primary somatosensory (SI) and primary motor cortex (MI). For over 30 years, PAE has been shown to produce a triad of symptoms (growth deficits, craniofacial dysmorphologies and central nervous system disruptions) diagnosed as fetal alcohol syndrome (FAS) in children and adults. Since low to moderate levels of prenatal alcohol exposure are less likely to produce FAS symptoms in children, the term “PAE” in this dissertation typically refers to heavy/abusive/chronic levels of prenatal alcohol exposure (in humans this equates to 5+ drinks/per …

Age-Associated Hepatic Drug Transporter Expression And Its Implications For Pediatric Pharmacotherapyflexibility Affect Dna Topoisomerase I Function, Lisa Tang

Theses and Dissertations (ETD)

Members of the ATP-binding cassette (ABC) family of drug transporter proteins translocate various endogenous and exogenous substrates across intra- and extracellular membranes. Two specific ABC transporters, the multidrug resistance 1/P-glycoprotein (MDR1/P-gp) and the multidrug resistance protein 2 (MRP2), serve as major hepatic transporters that mediate the biliary excretion of various organic anions and cations along with glutathione-, glucuronate-, or sulfate-conjugates of several drug substrates. However, very little is known about the expression of these transporters in the early infant and childhood ages of human development. We, therefore, characterized the ontogeny of these transporters by measuring their gene and protein expression. …

Development And Evaluation Of Brain Tumor Targeted Liposome Delivery System For Paclitaxel, Murali Krishna Divi

Theses and Dissertations (ETD)

Primary brain tumors are a relatively common cause of cancer-related deaths. High-grade gliomas are the most common type of primary brain cancer, and the affected patients have a median survival of less than 1 year. Almost all malignant gliomas are incurable with the present standards of healthcare. Currently accepted therapeutic adjuvants to surgery, such as radiotherapy and chemotherapy, provide only a minor improvement in the disease course and life expectancy for patients diagnosed with malignant gliomas. Often, chemotherapy has failed to make any significant impact on the prognosis of disease because of significant local and systemic toxicity, problems with transport …

The Cystic Fibrosis Transmembrane Conductance Regulator: Regulation By Hsp-90, Kevin Marrs

Theses and Dissertations (ETD)

The hypothesis of this project is that a functionally relevant association exists between CFTR and Hsp90. In testing this hypothesis, (a) the association of CFTR and Hsp90 is confirmed via crosslinking and immunoprecipitation of CFTR from stably transfected HEK-293 cells, electrophoretic separation of cross-linked proteins, and protein identification via SDS-PAGE, western blotting, and mass spectrometry. Also, this hypothesis is tested by (b) functionally assessing the effect of Hsp90 on CFTR function via Ussing chamber analysis, two-electrode voltage clamp analysis, preliminary in vivoanimal studies, and protein phosphorylation. The HEK-293 Flag-CFTR cells used in this study allows for a high degree …

Humanized Chimeric Receptors In The Therapy Of Multiple Sclerosis, Ioana Moisini

Theses and Dissertations (ETD)

The role of autoreactive, antigen-specific T-cells in the development of autoimmunity has long been documented. T-cells expressing chimeric receptors are specifically redirected against such cells and have been proven to suppress autoimmune encephalomyelitis, the murine model of multiple sclerosis. We here demonstrate the ability of humanized chimeric receptors to suppress experimental autoimmune encephalomyelitis (EAE) in a humanized mouse model by redirecting T lymphocytes against autoreactive T-cells. The receptors were synthesized by linking the 84-102 epitope of human myelin basic protein (MBP) to the extracellular and transmembrane domains of the beta chain of human major histocompatibility complex (MHC) class II molecule …

Dna Binding Specificity Of Mu Transcription Factor C And Crystallization Of C : Dna Complex, Karthik Shanmugantham

Theses and Dissertations (ETD)

The lytic cycle of phage Mu is regulated by a transcriptional cascade consisting of early, middle and late transcription. The Mor protein is an activator of the middle promoter Pm and is encoded by the last gene of the early transcript. The C protein is an activator of the four late promoters Plys, PI, PP, and Pmom and is expressed from the middle transcript. Both Mor and C proteins bind an imperfect dyad-symmetry element just upstream and overlapping the –35 region of Pm and Plys respectively. The main aims of this study was, (1) To understand the binding specificity of …

The Role Of Multi-Drug Resistance Associated Protein 4 And P-Glycoprotein In Resistance Of Neuroblastoma To Topotecan And Irinotecan, Patricia Kellie Turner

Theses and Dissertations (ETD)

High-risk neuroblastoma presents a significant therapeutic challenge because the 5-year survival rate remains less than 30% despite the use of surgery, multi-agent chemotherapy, radiation, and autologous bone marrow transplant. Novel therapeutic modalities are under development. The camptothecin analogs topotecan and irinotecan have been identified as successful cytotoxic agents. For topotecan, pharmacokinetically guided dosing to achieve a systemic exposure associated with preclinical anti-tumor activity in neuroblastoma xenograft models is feasible and has elicited favorable responses in children with high-risk neuroblastoma. However, some children with high-risk disease did not respond to the putatively effective topotecan systemic exposure. These children represent a subset …

Neuroadaptive Changes In The Mesocortical Glutamatergic System During Nicotine Self-Administration And After Extinction In Rats, Fan Wang

Theses and Dissertations (ETD)

The mesocorticolimbic pathway is critical in almost all aspects of drug abuse, including nicotine. Though many of the neurochemical and molecular effects of nicotine have been well studied, nicotine’s long-term neuroadaptive effects, specifically within the mesocorticolimbic pathway, are largely undefined. Thus, in current study, we determined the neuroadaptive changes in the mesocortical glutamatergic system during chronic nicotine self-administration (SA), which emulates important aspects of nicotine intake by humans, and after extinction. In the initial study, after 18 days of nicotine SA, in the medial prefrontal cortex (mPFC), NMDA receptor subunit 2A (NR2A) and NR2B were increased by 67% and 83%, …

Synthesis Of Novel Sulfonamide-Based Calpain Inhibitors And Their Potential As Anti-Tumor Agents, Jin Xu

Theses and Dissertations (ETD)

Calpain is a class of intracellular cytoplasmic cysteine proteases.1 The enzyme participates in different intracellular signaling pathways that are mediated by Ca2+.2 The two major isoforms of calpain universally distributed in most mammalian tissues are calpain 1 (µ-calpain) and calpain 2 (m-calpain). The exact in vivo function of the enzyme is not clear, but calpain has been implicated in a variety of physiological and pathological conditions,3 such as cancer, stroke, cardiac ischaemia, muscular dystrophy, cataract and Alzheimer’s disease. Calpain inhibitors are therefore of interest as therapeutic agents and as biomedical tools.

Several potent calpain inhibitors isolated from natural sources as …

Role Of Medial Prefrontal Cortical Group Ii Metabotropic Glutamate Receptor In The Development Of Cocaine Sensitization, Xiaohu Xie

Theses and Dissertations (ETD)

The current studies examined the role of medial prefrontal cortical (mPFC) group II metabotropic glutamate receptors (mGluR_2/3) in the development of cocaine sensitization. Initial studies demonstrated that intra-mPFC injection of the mGluR_2/3 receptor agonist, APDC, dose-dependently reduced acute behavioral response to cocaine (0.015-15 nmol/side with significant effects starting at 1.5nmol/side). The effects of APDC were prevented by intra-mPFC co-injections of an mGluR_2/3 antagonist, LY341495 (1.5 nmol/side). Repeated intra-mPFC APDC (1.5 nmol/side) injections also prevented the initiation of behavioral and neurochemical sensitization, which is defined as enhanced nucleus accumbens (NAc) dopamine response to cocaine. Once sensitization was …

Cardiopulmonary Predicators Of Dysfunctional Ventilator Weaning Response After Coronary Artery Bypass Graft, Annapoorna Mary

Theses and Dissertations (ETD)

Although the majority of coronary artery bypass graft (CABG) surgery patients are extubated within 6 to 8 hours following surgery, 20% to 40% of patients remain intubated 12 hours after surgery due to dysfunctional ventilator weaning response (DVWR). DVWR associated with increased morbidity and mortality (30% to 43%) following CABG surgery. Finding significant antecedence to predict DVWR could help to identify and prevent the complications from DVWR after CABG surgery. Literature review revealed that there is an association between cardiopulmonary indicators (CPI) and DVWR after CABG surgery. Cardiopulmonary indicators are the selected hemodynamic parameters that have an association with DVWR. …

Relationship Between Premenstrual Symptom Severity And Catastrophic Thinking, Candon Deanne Norton

All-Inclusive List of Electronic Theses and Dissertations

Premenstrual Dysphoric Disorder (PMDD) is proposed as a cluster of severe emotional, physical and behavioral symptoms that affect a small percentage or women during the late luteal phase of their menstrual cycle and cease shortly after the onset or menses. Although some researchers hypothesize there is a difference in the amount or pain actually experienced during the premenstrual phase, others argue that there muy be differences in how women interpret the 'normal' fluctuations and bodily changes of the premenstrual phase. Perhaps women who experience severe premenstrual symptoms anticipate their symptoms and become hypervigilant in monitoring normal bodily sensations, which results …

Theoretical And Experimental Evaluation Of Spatial Resolution In A Variable Resolution X-Ray Computed Tomography Scanner, Roman Melnyk

Theses and Dissertations (ETD)

A variable resolution x-ray (VRX) computed tomography (CT) scanner can image objects of various sizes with greatly improved spatial resolution. The scanner employs an angulated discrete detector and achieves the resolution boost by matching the detector angulation to the scanner field of view (FOV) determined by the size of an object being imaged. A comprehensive evaluation of spatial resolution in an experimental version of the VRX CT scanner is presented in this dissertation. Two components of this resolution were evaluated – the pre-reconstruction spatial resolution, described by the detector presampling modulation transfer function (MTF), and the post-reconstruction spatial resolution, given …

Influence Of Stroke Clinical Pathway On Documentation, Shannon Sternberg

All Theses

The purpose of this study was to determine whether the implementation of a stroke clinical pathway could improve compliance with documentation of specific quality measures for patients admitted with the diagnosis of acute ischemic stroke, hemorrhagic stroke, or transient ischemic attack. The study used the ten standardized performance measures for stroke identified by the Joint Commission on Accreditation of Healthcare Organizations (JCAHO) and integrated documentation of those measures into a stroke clinical pathway consisting of physician order sets, an interdisciplinary plan of care, and a patient/family education flow sheet. The American Stroke Association's Get with the Guidelines - Stroke (GWTG-Stroke) …

Conferred Immunity Against The H1n1 Type A Influenza Virus In Madin-Darby Canine Kidney Cells Via Cecropin B Transfection Therapy., Tomasz P. Stryjewski

Honors Capstones

No abstract provided.

Effects Of Delayed Auditory Feedback On The Bereitschaftspotential, Jennifer L. Johnson

Theses and Dissertations

This study examined the brain electrical activity of normal speakers in a non-delayed auditory feedback (DAF) condition and when experiencing DAF to determine the effect DAF would have on the Bereitschaftspotential (BP). The BP reflects the preparatory state of a person prior to motor execution of an act and can be observed 1500 to 500 ms prior to voluntary movement. The participants in the study included 10 adults with normal speech. Each read a series of 30 sentences, both without DAF and with DAF, while the BP was measured. Results indicate that the BP is present across the scalp in …

Split-Half Reliability Of Mlu And Mlu2 In Two Methods Of Utterance Segmentation, Alyse Diana Kemeny

Theses and Dissertations

Concerns regarding Mean Length of Utterance (MLU) has led to adaptations of this method of analysis. A recent study by Johnston (2001) introduced an alternative to MLU called MLU2. The current study investigates the split-half reliability of MLU and MLU2 as well as another alternative, Median Length of Utterance (Med-LU). Split-half reliability was found for these methods when segmented into Phonological and Communication Units. Split-half reliability of MLU2 was generally higher than that of MLU, and both were higher than Med-LU. The study suggests that MLU2 may also be a valuable tool for clinicians in analyzing child language.

Psychometrically Equivalent Cantonese Bisyllabic Word Recognition Materials Spoken By Male And Female Talkers, Brooke Kristin Conklin

Theses and Dissertations

The purpose of this study was to create psychometrically equivalent word lists in the language of Cantonese for word recognition testing. Frequently used bisyllabic Cantonese words were recorded by a native female and male talker. The word lists were evaluated by administering the word recognition lists to 20 native speakers of Cantonese with normal hearing. Each list was presented at 10 different intensity levels ranging from -5 to 40 dB HL in 5 dB increments. Logistic regression was used to determine the words with the steepest logistic regression slopes. The 200 words with the steepest slopes were then formulated into …

Graduate Project To Explore Factors Affecting Completion Of Final Degree Requirements For A Master Degree In Nursing, Sharon Gentile

Theses and Graduate Projects

The purpose of this project was to explore issues that influence Master Degree completion as perceived by graduate nursing students, using focus groups. All eight graduate nursing student participants recently completed structured coursework, but had not submitted a final paper or project. Margaret Newman's Health as an Expanding Consciousness Nursing Theory was applied as the conceptual framework used to process the information obtained. Dialogue between moderator and participants occurred during accompaniment enabling valuable information to be elicited, and participants' personal clarification of concerns; therefore establishing praxis. Issues emerged related to adult learners seeking defined expectations and effective relationships with advisors. …

Inter- And Intra-Subject Variability: A Palatometric Study, Marybeth Corey Sanders

Theses and Dissertations

Electropalatometry (EPM) has proven to be a useful clinical and research tool for measuring tongue-to-palate contact. The goal of this study was to determine whether the development of a database of standardized palatometric articulation files is feasible by examining the variability which exists within and between speakers. Twenty standard American English dialect speakers were fitted with palatometer pseudopalates. Test stimuli were VCV nonsense words using a schwa in the initial position, the 15 palatal consonants, and three corner vowels, /a/, /i/, /u/. From these palatometric recordings a variability index was created to examine intra- and interspeaker variability. Different aspects of …

Using Art In Occupational Therapy With People Who Have Cancer: A Qualitative Case Study, Jennifer R. Dochod

Masters Theses

OBJECTIVE. To explore the role of art, with a small group of people who have cancer, as a means provide distraction from pain, to help express feelings, and to assist in making connections with others.

METHOD. Using a randomized sample from Gilda’s Club, a support center for those with cancer, in-house social workers recruited two participants to take part in a small group case study conducted by a master’s level occupational therapy student.

RESULTS. Two participants enrolled in the art class conducted by the researcher. Predetermined questions gathered through participant interviews and class discussions provided the data. Although the participant …

The Effectiveness Of Prophylactic Eye Drops On The Reversal Of Uv-Light Induced Cataracts, Contessa Bly Bowman

Theses and Dissertations

Cataracts are a cloudiness of the lens within the eye which obstructs vision. They are found in many species including rats, dogs, and humans. Vision is hindered where this "cloudiness", made of water build up and broken crystalline, proteins take form. Oculvet is an eye medication, used by veterinarians, containing carnosine as its active ingredient. Topical application of carnosine is reported to be an effective treatment for the prevention and removal of pre-existing cataracts. For this experiment, Sprague- Dawley rats were anesthetized and exposed to ultraviolet radiation, UVB and UVC. They were divided into four groups (n = 32), three …

Antimicrobial Activity Screening Of Recombinant And Synthetic Varasin A Defensin From The Hard Tick Dermacentor Variabilis Against Various Bacteria, Julia A. Sharp

Biological Sciences Theses & Dissertations

Hematophagous arthropods, such as ticks and mosquitoes, rely on their innate immune system for defense against pathogens ingested in a blood meal as well as those acquired through injury. In response to pathogen recognition, the production of antimicrobial peptides, such as defensin, is typically upregulated. Varisin, a defensin, is thought to be a key component in the immunocompetence of the hard tick D. variabilis against Borrelia burgdorferi, the causative agent of Lyme disease. To study the antimicrobial effects of varisin, recombinant varisin was expressed by both insect cells and E coli. Purification of the protein followed by enterokinase treatment yielded …

Simulation To Augment Standardized Patients In Obstetric Ultrasound Training, Scott Richard Guirlinger

Computational Modeling & Simulation Engineering Theses & Dissertations

Standardized patients are actors used in medical training to teach and eval11atc clinical skills of students prior to their using these skills on an actual patient. Augmented Reality is a relatively new technology that can be used to augment standardized patients with abnonnalitie8 and pathologies that they cannot portray themselves as normal healthy human beings. In our project, we sought to augment standardized patients with a 12-week- old fetus in order to train and possibly evaluate medical students and professionals in performing obstetric ultrasound procedures and interpreting the corresponding ultrasound image results.

The software library ARToolkit provided the framework for …

Microfabrication Of An Optical Chamber For Exposing Living Cells To Pulsed Electric Fields, Saleh Sheikh

Electrical & Computer Engineering Theses & Dissertations

I have fabricated a chamber which enables the application of Nanosecond Pulsed Electric Fields (nsPEFs) to living cells while monitoring their real time fluorescence with an inverted microscope. The No. I thickness (150µm nominal) coverslip glass substrate used for fabrication allows the use of high numerical aperture oil immersion objectives for imaging intracellular organelles. Electrodes are fabricated by first evaporating a chromium adhesion layer, followed by a nickel seed layer. Subsequently, an ultra-thick positive photoresist is applied and patterned to create a 30µm thick electroplating mold. The electrodes are then electrodeposited into the mold from a nickel sulfamate plating bath. …

Prevalence Of Stress, Urge, And Mixed Urinary Incontinence In Women, Chaandini Jayachandran

Master's Theses and Doctoral Dissertations

Urinary Incontinence is a common affliction that impairs quality of life and restricts social activity in adult women. Based on etiology and pathophysiology, urinary incontinence is classified into Stress, Urge, and Mixed urinary incontinence. Knowledge of the specific type of incontinence helps in determining course of treatment. A retrospective, descriptive, observational study was designed to determine the percentage of new patients seeking treatment for bladder complaints, who were identified by Medical, Epidemiological, and Social aspects of Aging (MESA) questionnaire to reflect Stress, Urge, or Mixed urinary incontinence. The secondary aim was to investigate any trends other variables studied that showed …

The Skeletal Effects To The Maxilla After Rapid Maxillary Expansion Using Cbct, Brett J. Garrett

Loma Linda University Electronic Theses, Dissertations & Projects

The purpose of this study was to use cone beam computed tomography (CBCT) to quantitatively evaluate and compare skeletal expansion and alveolar tipping of the maxilla at the maxillary canine (Cl), first premolar (PI), second premolar (P2) and first molar (Ml) after rapid maxillary expansion (RME). Transverse effects to the maxillary suture, nasal width and maxillary sinus were also assessed. Included in the study were thirty consecutive patients (17 males and 13 females; mean age = 13.8 + 1.7 years) requiring RME using Hyrax appliances as part of their comprehensive orthodontic treatment. The pre-RME and post-RME measurements of palatal and …