Medicine and Health Sciences Commons^™

Nucleoplasmic And Cytoplasmic Degradation Of Telomerase: Implications Toward Telomerase-Based Cancer Therapy, Binh Nguyen

Theses and Dissertations

Telomerase is a ribonucleoprotein that is reactivated in cancer cells to allow for continuous cellular division and indefinite growth. With telomerase being expressed in more than 85% of all cancer, it is imperative that we understand how to selectively inactivate and degrade this unique DNA polymerase. In doing so, we can specifically target tumor cells to erode their telomeres so that they will undergo apoptosis or senescence. Through this research, we have learned that telomerase can be degraded in the nucleoplasm by Hsp90 chaperone inhibition and in the cytoplasm by the dominant negative mutant, D712A V713I. These findings should guide …

Three Models Of Anthrax Toxin Effects On The Map-Kinase Pathway And Macrophage Survival, Daniel J. Schneider

Theses and Dissertations

Lethal factor (LF), a component of anthrax toxin, is the primary virulence factor that allows Bacillus anthracis to evade the immune response by blocking the activation of mitogen-activated protein kinase (MAPK) enzymes. This research modifies three published MAPK models to reflect this signal inhibition and to estimate a first-order reaction rate by fitting the models to published viability data for two macrophage cell lines cultured with the LF-producing Bacillus anthracis-Vollum1B strain. One model appears to be ill-suited for this purpose because not all relevant MAPK components could be integrated into the inhibition equations. Despite different underlying parameters and values, the …

Micro-Etched Platforms For Thermal Inactivation Of Bacillus Anthracis And Bacillus Thuringiensis Spores, Leslie S. Hawkins

Theses and Dissertations

This research developed and applied microlithography techniques to etch microscope slide platforms in order to measure thermal inactivation of Bacillus thuringiensis and Bacillus anthracis spores, two closely related Bacillus species. B.t. is widely used as a surrogate for B.a. in response studies and in some field studies. This work addressed the previously identified problem of measuring both spore growth and spore thermal kill threshold using the traditional method of spreading a diluted spore solution on a plate. The micro-etched platforms forced spore separation thereby preventing neighbor growth from obscuring germination and initial vegetative growth measurements using a microscope. The technique …

Struggling With Disconnection: The Lived Experience Of Being Homeless And In Need Of Healthcare, Nonceba Mbangata

Theses and Graduate Projects

The intent of this field project was to study the disconnection that is experienced by homeless persons within the healthcare system. Homelessness is a growing concern that affects all age groups throughout the United States. It is not a secret that the increase in homelessness is stressing the health care system and limiting choices of becoming a healthy individual. The state of the health care system today and the nomadic habits of homeless persons make it impossible for those struggling with homelessness to stay connected to health care services. This project will illuminate the lived experiences of homeless persons as …

Accuracy Of Automated Analysis Of Language Samples From Persons With Deafness Or Hearing Impairment, Anne M. Hasting

Theses and Dissertations

Developmental Sentence Scoring (DSS) and the Language Assessment, Remediation, and Screening Procedure (LARSP) are among the more common analyses for syntax and morphology, and automated versions of these analyses have been shown to be effective. This study measured the accuracy of automated DSS and LARSP on the written English output of six prelingually deaf young adults, ranging in age from 18 to 32 years. The samples were analyzed using the DSS and LARSP programs on Computerized Profiling; manual analysis was then performed on the samples. Point-by-point accuracy for DSS and for each level of LARSP was reported. Characteristics of the …

The Effects Of Deep Brain Stimulation On The Speech Of Patients With Parkinson's Disease, Erin Suzanne Bjarnason

Theses and Dissertations

Deep brain stimulation (DBS) of the subthalamic nucleus (STN) has received more attention in recent years as a treatment option for regulating the symptoms of Parkinson's disease. Previous studies of DBS documented consistent improvements in motor function but more variability in speech outcomes. In the present study, six participants diagnosed with idiopathic Parkinson's disease who reported worsened speech with stimulation were recorded performing speech acoustic tasks with the stimulators on, and again with the stimulators off. Improvements were noted for most participants in measurements of formant slopes, long term average spectrum (LTAS) of a sustained vowel, and spirantization with stimulation …

The Effect Of Regional Dialect On The Validity And Reliability Of Word Recognition Scores, Jamie Ann Garlick

Theses and Dissertations

The purpose of this study was to examine the effect of talker and listener dialect on the validity and reliability of word recognition scores from two sets of Mandarin speech audiometry materials. Four lists of bisyllabic words in Mainland Mandarin and Taiwan Mandarin dialects were administered to 16 participants of each dialect with normal hearing across two test sessions. The performance on materials presented in the native dialect was compared to performance on non-native dialect assessment to determine validity and reliability of test materials. Statistical analysis indicated significant differences between word recognition scores across test sessions, talker and listener dialect, …

Event Related Potentials Of Syntactic Language Processing In Two Children With Specific Language Impairment: A Case Study, Melissa Ann Willes

Theses and Dissertations

This study examined the electrophysiological activity of two children with Specific Language Impairment (SLI) and two aged-matched peers while listening to syntactically correct sentences versus syntactically incorrect sentences. The study specifically analyzed the N400 and P600 components. The N400 component is a negative wave occurring approximately 400 ms post-stimulus and is elicited by semantically incorrect stimuli. The P600 component is a positivity that occurs approximately 600 ms post-stimulus and reflects processing of syntactically incorrect stimuli. The participants in the study included a 7-year-old child and a 9-year-old child with SLI and two age-matched peers with typically developing language. Each participant …

The Effect Of Non-Native Dialect On Speech Recognition Threshold For Native Mandarin Speakers, Nathan Edward Richardson

Theses and Dissertations

Speech recognition thresholds are used for several clinical purposes, so it is important that they be accurate reflections of hearing ability. Variations in the acoustic signal may artificially decrease threshold scores, and such variations can result from being tested in a second dialect. Thirty-two native Mandarin-speaking subjects (sixteen from mainland China and sixteen from Taiwan) participated in speech recognition threshold testing in both dialects to see whether using non-native dialect test materials resulted in a significantly lower score. In addition, tests were scored by two interpreters, one from each dialect, to see whether the scorer's dialect resulted in a significantly …

In Vitro Toxicity Of Aluminum Nanoparticles In Human Keratinocytes, Stephanie Mccormack-Brown

Theses and Dissertations

Nanotechnology promises to be the defining technology of the 21st century. At an annual investment of $1B, it provides significant contributions to manufacturing, medicine, energy conservation, and the environment. Nanoparticles are structures with at least one dimension in the 1 to 100 nanometer (nm) range. DoD and US Air Force interest in aluminum nanoparticles (AL NPs) stems from its ability to enhance combustion jet fuel, thus increasing fuel efficiency. The addition of AL NPs to JP-8 may pose a unique dermal hazard to aircraft maintenance workers. There is no published data on AL NP toxicity effects on human skin. This …

The Development Of Intentionality, Vocabulary, And Grammar In A Child With Autism, Heidi Huckabee

Honors Capstones

No abstract provided.

Precision Of Temporary Anchorage Device Placement With 2d Vs. 3d Diagnostic Tools And Surgical Guides, Howard Lee

Loma Linda University Electronic Theses, Dissertations & Projects

Current methods of temporary anchorage device (TAD) planning and placement are performed by radiographic analysis in a two-dimensional plane that lends itself to inaccuracy secondary to variations in the angle from which the radiograph is taken, as well as the magnification of the radiographic image, resulting in an increased likelihood of placement errors. The purpose of this study was to compare a three-dimensional (3D) TAD planning and placement technique to a traditional two-dimensional (2D) technique. In the 2D group, periapical radiographs and pre-fabricated wire guides were used for planning and placement of TADs on five plastic models. For the 3D …

Radiation-Induced Glutamate Transport Alterations In Neuron-Astrocyte Coupling, Martha Celia Sanchez

Loma Linda University Electronic Theses, Dissertations & Projects

Exposure of the central nervous system (CNS) to ionizing radiation is known to result in behavioral, cognitive, and motor deficits - effects similar to those seen in many neurodegenerative disorders. Neurons and astrocytes, two principal cell types in the brain, coexist as an interdependent metabolic unit via the neurotransmitter glutamate. Disruption of this metabolic coupling would have widespread effects within the CNS, therefore it is hypothesized that ionizing radiation impairs glutamate transport and metabolism, and increases oxidative stress, ultimately impairing neuron-astrocyte coupling. We propose to investigate the mechanism and determine the impetus for radiation-induced neurotoxicity by measuring the temporal sequence …

Influence Of Mandibular Fixed Retainers On Diagnostic Quality Of Cranial Mri, Norman E. Carter

Loma Linda University Electronic Theses, Dissertations & Projects

The purpose of this study was to evaluate the diagnostic quality of MR images taken on subjects with different types of orthodontic lingual retainers in place. The study provides guidelines and recommendations for use of lingual retainer materials in those patients who are or may potentially undergo routine magnetic resonance imaging. Ten subjects had impressions taken and models made for retainer tray fabrication. Removable trays with each lingual retainer material incorporated in them were fabricated from the models. Subjects underwent scans with each of the five test trays in place. T1 sagittal (T1), T2 axial (T2), gradient echo (GRE), and …

Memory For Emotional Images : Recognition And Temporal Contexts, George E. Devore

Loma Linda University Electronic Theses, Dissertations & Projects

Negative emotional stimuli are usually better remembered than neutral emotional stimuli. Previous examination of binding theory found no differences in recall for homogeneous lists of taboo and neutral words. The current study expands on binding theory using negative and neutral visual stimuli in homogeneous lists. A rapid serial visual presentation paradigm and recognition and temporal memory item-discrimination tasks were used. Binding theory predicts no differences between negative and neutral images for recognition and temporal memory. Results were consistent with the assumptions of binding theory and no significant differences were found. This result suggests that binding theory generalizes for visual stimuli …

Infant Feeding Decisions Among Pregnant Black Wic Participants And The Role Of Peer Support, Astrid D. Mickens

Loma Linda University Electronic Theses, Dissertations & Projects

The purpose of this study was to identify the important factors that impact infant feeding decisions among Black women, and to determine whether peer support influences the relationship among theoretical constructs of the social learning theory. A convenience sample of 110 Black pregnant women, ages 18-45, attending Women, Infant and Children (WIC) clinics throughout Riverside and San Bernardino Counties were recruited to participate in the study through the Nutrition Services Department. Eligible participants included pregnant Black WIC clients in any stage of pregnancy.

The survey questions contained information about perceived support, cognitive knowledge, abilities and beliefs regarding breastfeeding. The goal …

Ultra-Fine Particle Formation Using Principle Of Rapid Expansion Of Supercritical Solutions`, Miraj Minesh Sheth

Theses

There are indications in the chemical and pharmaceutical industries that the reduction in size of a crystalline particle can lead to better performance of the drug compound, particularly for water insoluble drugs, in the final dosage form. Many particle formation techniques have been investigated in recent years by researchers to obtain desired particulate sizes and size distributions. Supercritical fluid technologies have been successfully investigated for particle formation due to its unique gas/liquid properties in the supereritical state. In this report, results of particle formation using the principles of Rapid Expansion of Supercritical Solutions (RESS) have been documented.

In the RESS …

Long Driving Hours And Health Of Truck Drivers, Stephen J. Benstowe

Theses

In recent time, the health of truck drivers has become a concern for regulatory agencies and safety professionals all over the world. Fatal and non-fatal injury rates for truck drivers are among the highest of all occupations. Truck driving is an important and tedious job. Driving for long hours, drivers are confined to a small space, sit with static lower and upper extremities posture, mentally focus and absorb vibrations.

This thesis provides an in-depth review of the literature related to the problems of long distance truck drivers and commercial motor vehicle operators. The Literature suggests that continuous exposure of truck …

A Comparison Of Perinatal Care Providers' Use Of The National Institute Of Child Health And Human Development Standardized Terminology In Documentation Of Intrapartal Fetal Heart Rate Patterns, Faye M. Sigman

Dissertations

The purpose of this study was to determine if perinatal team members; nurses (RN) and primary care providers (PCP), were using the NICHD standardized terminology to document Fetal Heart Rate patterns during labor. Agreement in documentation of FHR and agreement in concept between the RN and PCP was also studied. A descriptive, comparative research design was used. Cohen?s Kappa statistics measured agreement in documentation of FHR patterns and Chi square measured agreement in concept, p< 0.05 for each. A retrospective medical records chart review was performed on 400 charts, meeting inclusion criteria, from three community hospitals. There were three data collection points and four criteria reviewed. This study found the use of NICHD terminology to document FHR alarmingly low (RN=51%; PCP=13%). It was used most often for decelerations (81%) RN, (22%) PCP, and least often for variability (19%) RN, (3%) PCP. Incomplete documentation was extremely high for the PCP (69%) and 81 charts (20%) had no FHR documentation. Agreement in documentation varied between the RN and PCP. They agreed most often on accelerations (81.4%) and least often on baseline rate (41.5%). When looking at all there points in time the RN and PCP agreed in documentation 59% but agreed in concept 78%. There were four areas where the RN and PCP agreed in their use of NICHD terminology: Accelerations on admission n=151, Kappa=0.091, p=0.007; variability during labor n=68, Kappa=0.27, p=0.015; variability prior to delivery n=33 Kappa=0.33, p=0.010, and decelerations during labor n=103, Kappa=0.16, p=0.018. Data from this study supports expanding this research, to identifying barriers to documentation. It also appears that education in use of NICHD terminology is needed.

Knowledge And Utilization Of The Perioperative Nursing Data Set By Perioperative Staff Nurses In Southeastern Michigan, Jill Ortmann

Master's Theses and Doctoral Dissertations

The purpose of this descriptive/correlational study was to describe what perioperative nurses practicing in southeastern Michigan know about the Perioperative Nursing Data Set (PNDS), how they are utilizing the PNDS, and their opinion of the PNDS, and also to examine the relationship between these variables and demographic data. Four research questions were addressed by the use of a questionnaire.

The results of this study were drawn from the return of 151 questionnaires out of a possible 319 questionnaires submitted to practicing perioperative registered nurses (RNs) in southeastern Michigan (47 percent return rate). These results showed that overall, perioperative RNs did …

Role Of Transient Receptor Potential Canonical-6 (Trpc6) Channel In Metastasis Of Glioblastoma Multiforme, Rajarajeshwari Venkataraman

Electronic Theses and Dissertations

Glioblastoma multiforme (GBM) is one of the extremely fatal brain tumors. The main reason that makes it so lethal is its capability to invade and spread to other parts of CNS producing secondary tumors. Among other factors hypoxia, reduced oxygen availability, is linked to higher metastatic potential of cancers. Hypoxia causes numerous changes in genome and proteome of the cell. These changes help a normal cell to adapt to nutritional deficiency, but the same changes can increase the malignancy and metastasis in tumor cells. Extensive research by a number of curious scientists reveal that various pathways involving numerous proteins cross-talk …

Evaluation Of The Efficacy Of Chloroplast-Derived Antigensagainst Malaria, Melissa Schreiber

Electronic Theses and Dissertations

Malaria is the most prevalent vector-borne parasitic disease worldwide and a major cause of death from infections. There is a great need to develop a low cost vaccine for malaria to control transmission of infection and impact of disease, due to the emergence of anti-malarial resistance. Two leading blood stage malarial vaccine candidates are the apical membrane antigen-1 (AMA-1) and the merozoite surface protein-1 (MSP-1). The aim of this project is to express malarial antigens in tobacco plants via plastid transformation and deliver them by subcutaneous or oral gavage of minimally processed transplastomic tissue to evaluate their efficacy to elicit …

Understanding The Nature And Impact Of Alcoholism : Implications For Ministry In Kenya, Margaret Kemunto Obaga

Master of Theology Theses

No abstract provided.

An Occupational Therapist's Guide For Rehabilitative Driving With Traumatic Brain Injured Clients, Josiah Hadsall

Occupational Therapy Capstones

Traumatic brain injuries are devastating occurrence accounting for nearly 10 million injuries occurring each year, with 2 million of those occurring in the United States. As these individuals progress through rehabilitation and begin to acquire independence once again, they look for opportunities to reintegrate within the communities which they live. Driving has been identified as a monumental stage of rehabilitation and is a key way to experience the community for individuals after a traumatic brain injury. This scholarly project was conducted to help occupational therapists addressing driving rehabilitation with traumatic brain injured clients and help ease some of the problems …

Mental Health And Business Professionals’ Employment-Related Perceptions Of Individuals With Psychological Disorders, Kevan Mock

Theses, Dissertations and Capstones

Mental health and business professionals’ employment-related perceptions of 6 psychological disorders (i.e. alcoholism, insomnia, major depression, social phobia, post- traumatic stress disorder, obesity) were examined. The 33 professionals (n = 18 mental health; n = 15 business) evaluated each disorder on 18 employment-related dimensions (e.g. employability, productivity, trainability). Specifically, they evaluated the perceived likelihood of each of the 18 employment-related dimensions being associated with each of the 6 psychological disorders (1 = not likely; 5 = highly likely). Perceptions of the 33 mental health and business professionals were compared with the perceptions of college students (n = 106) obtained in …

Effects Of Aging And Gender On Regulators Of Muscle Adaptation In F344/Bn Rat Model, Satyanarayana Paturi

Theses, Dissertations and Capstones

Sarcopenia is the loss of muscle mass and strength that occurs with aging. Here we examine the effects of aging and gender on the regulation of molecules believed to regulate muscle growth and adaptation in the F344/BN rat. In male animals, soleus and EDL muscle/body weight ratio declined continuously with aging while muscle atrophy in female animals plateaued at 26-months and remained constant thereafter. Aging increased the phosphorylation of protein kinase-B (Akt) and the mammalian target of rapamycin (mTOR) in the female but not male soleus muscle. This finding was associated with the attenuation of muscle atrophy observed in female …

Manganese Superoxide Dismutase Contributes To Cisplatin-Induced Cytotoxicity Rather Than All-Trans Retinoic Acid-Dependent Chemoresistance, Zina-Ann Cardozo

Theses, Dissertations and Capstones

Neuroblastoma is an extra-cranial solid tumor of the nervous system occurring predominantly in infants and children younger than five years of age. Neuroblastoma presents a challenge to therapy primarily due to a decreased responsiveness to anticancer agents like cisplatin (CDDP), leading to recurrence. Vitamin A and its derivatives, known as retinoids, are preventive against cancer and induce differentiation in some cell lines. Retinoids are now being tested clinically for the treatment of neuroblastoma. One major limitation to retinoid therapy is the development of chemoresistance. In the present study human SK-N-SH neuroblastoma cells pretreated with 10 μM all-trans retinoic acid (ATRA) …

Factors That Influence The Distribution Of Antibiotic Resistant Bacteria In The Mud River, Wv, Timothy Shaun Dotson

Theses, Dissertations and Capstones

Background: Prior studies of the Ohio and Mud Rivers have shown that fecal contamination alone does not explain the distribution of antibiotic resistant bacteria (ARB) in surface waters. The objectives of this study were to determine effects on the distribution of ARB in the Mud.

Methods: Water samples and physical parameters were collected twice per season in spring through fall at 12 sites on the Mud River. Aliquots were plated on R2A agar and R2A agar plus individual antibiotics for the enumeration of total cultivable bacteria, and cultivable bacteria resistant to ciprofloxacin, virginiamycin, or tetracycline respectively. The IDEXX Quanti-Tray/2000™ method …

Impaired Overload-Induced Hypertrophy In Obese Zucker Rat Slow-Twitch Skeletal Muscle, Anil Kumar Gutta

Theses, Dissertations and Capstones

How insulin resistant muscle responds to mechanical overload (MOV) is not well understood. Using immunohistochemical analysis and immunoblotting, we investigated whether Lean Zucker (LZ) and insulin resistant Obese Zucker rats (OZ) respond in a similar fashion to MOV. Five young adult (2 months old) male LZ and OZ rats were subjected to a surgical ablation of the gastrocnemius muscle and overloaded for 8 weeks. MOV-induced increases in soleus muscle mass and average fiber cross-sectional area were attenuated in OZ compared to LZ animals. This reduction in OZ muscle adaptation was associated with decreased activity / phosphorylation levels of STAT3, β-catenin …

Generation Of Recombinant Mouse Embryonic Stem Cell Lines And Theirapplication For In Vivo Bioluminiscence Imaging In The Heart, Ramana Kammili

Electronic Theses and Dissertations

Cardiovascular disease is the major cause of death in the United States, with 80 million people suffering from some form of heart disease each year. One major limitation is the inability of the heart to repair the damaged tissue. Stem cell therapy holds enormous promise to repair and regenerate the damaged myocardium, but there are many technical difficulties that must first be overcome. One such difficulty is the present lack of ability to track and assess transplanted stem cells over time in vivo. The central hypothesis of this thesis is that in vivo bioluminescence imaging is a safe and useful …