Medicine and Health Sciences Commons^™

Mid-Atlantic Ethics Committee Newsletter, Spring 1994

Mid-Atlantic Ethics Committee Newsletter

No abstract provided.

Use Of In Vivo Complementation In Mycobacterium Tuberculosis To Identify A Genomic Fragment Associated With Virulence, Lisa Pascopella, Frank M. Collins, Jean M. Martin, Mong Hong Lee, Graham F. Hatfull, C. Kendall Stover, Barry R. Bloom, William R. Jacobs Jr.

Public and Community Health Sciences Faculty Publications

Novel molecular tools and genetic methods were developed to isolate genomic fragments of Mycobacterium tuberculosis that may be associated with virulence. We sought to restore virulence, a characteristic of M. tuberculosis that is correlated with growth rate in mouse spleen and lung tissue, to the avirulent strain H37Ra by complementation. A representative library of the virulent M. tuberculosis strain H37Rv was constructed and transformed into H37Ra. Enrichment for individual faster-growing recombinants was achieved by passage of pools of H37Ra transformants harboring the H37Rv library through mice. A molecular strategy was devised to isolate and clone the H37Rv genomic DNA fragment …

Factors Altering Aneurysm Wall Strain, George M. Austin

The Dr. George M. Austin Neurology Research Collection

Factors Altering Aneurysm Wall Strain. George M. Austin, M.D. Poster presentation. American Association of Neurological Surgeons, San Diego Convention Center. April 9-14, 1994. 1 pg.

Ua66/14/4 Kentucky Partnership Newsletter, Vol. Ii, No. 2, Kentucky Partnership For Farm Family Health & Safety

University Organizations

Newsletter created by and about the Kentucky Partnership for Farm Family Health & Safety.

Menopausal Hormone Replacement: Not Necessarily Routine, Mary Guinan

School of Public Health Faculty Research

No abstract provided.

An Ethicist's Commentary On Whether Veterinarians Should Report Cruelty, Bernard E. Rollin

Animal Welfare Collection

No abstract provided.

Preimplantation Development Of In Vitro-Matured And In Vitro-Fertilized Ovine Zygotes: Comparison Between Coculture On Oviduct Epithelial Cell Monolayers And Culture Under Low Oxygen Atmosphere., A J Watson, P H Watson, D Warnes, S K Walker, D T Armstrong, R F Seamark

Obstetrics & Gynaecology Publications

The roles of medium composition, serum source, embryo coculture, and culture under low O2 conditions on the development of in vitro-matured and in vitro-fertilized (IVMF) ovine zygotes were investigated in three separate experiments. In the first experiment, the proportion of cocultured IVMF zygotes developing to the blastocyst stage was significantly higher (38.0% vs. 3.5%; p < 0.05) than that of non-cocultured zygotes treated within three embryo culture media (TCM-199 + 10% fetal bovine serum [FBS]; bicarbonate-buffered, glucose-free synthetic oviduct fluid medium [mod-SOFM] + 10% FBS; and bicarbonate-buffered BSA-free Tyrode's salt solution [mod-TALP] + 10% FBS) under a 5% CO2 atmosphere in air. In a second experiment, a significantly higher (p < 0.05) proportion of cocultured zygotes placed in TCM-199 medium survived to the blastocyst stage (37.4% blastocysts vs. 23.4% in mod-SOFM). No significant effect of serum (FBS vs. human serum [HS]) was observed on embryonic development, but coculture was confirmed to exert a significant influence on development to the blastocyst stage. In the final experiment, survival of the embryo under a reduced oxygen (5% CO2:5% O2:90% N2) atmosphere was investigated. In contrast to results in the initial experiments, embryonic survival was significantly higher (p < 0.05) in the non-cocultured treatment groups (21.9% blastocysts vs. 0.4% for cocultured zygotes). Serum source also had a significant (p < 0.05) influence upon the development of non-cocultured zygotes: 32.3% of zygotes cultured with HS progressed to the blastocyst stage vs. 11.5% of zygotes cultured in FBS-supplemented medium. These results have characterized two distinct culture environments, each capable of supporting the development of high frequencies of unselected IVMF zygotes to the blastocyst stage in vitro.

A Growth Factor Phenotype Map For Ovine Preimplantation Development., A J Watson, P H Watson, M Arcellana-Panlilio, D Warnes, S K Walker, G A Schultz, D T Armstrong, R F Seamark

Obstetrics & Gynaecology Publications

The reverse transcription-polymerase chain reaction (RT-PCR) was used to determine the patterns of expression for several growth factor ligand and receptor genes during ovine preimplantation development. Transcripts for insulin-like growth factor (IGF)-I, IGF-II, and the receptors for insulin and IGF-I were detected throughout ovine preimplantation development from the 1-cell to the blastocyst stage. Transforming growth factor alpha (TGF alpha) transcripts were also detected throughout ovine preimplantation development. The mRNAs encoding basic fibroblast growth factor (bFGF) were detected in all stages of the ovine preimplantation embryo, although the relative abundance of this transcript consistently decreased from the 1-cell to the blastocyst …

Ua12/4 Commuter Link, Vol. 2, No. 2, Wku Health Services

WKU Administration Documents

Newsletter created for non-traditional students returning to WKU.

Cryptic Disseminated Tuberculosis: An Often Missed Diagnosis, A Jabbar

Section of Pulmonary & Critical Care

No abstract provided.

A Tension Between Two Cultures ... Dentistry As A Profession And Dentistry As Proprietary, David A. Nash

Oral Health Science Faculty Publications

No abstract provided.

Phylogenetic Associations Of Human And Simian T-Cell Leukemia/Lymphotropic Virus Type I Strains: Evidence For Interspecies Transmission, Igor J. Koralnik, Enzo Boeri, W. Carl Saxinger, Anita Lo Monico, Jake Fullen, Antoine Gessain, Hong-Guang Guo, Robert C. Gallo, Phillip Markham, Vaniambadi Kalyanaraman, Vanessa Hirsch, Jonathan Allan, Krishna Murthy, Patricia Alford, Jill Pecon-Slattery, Stephen J. O'Brien, Genoveffa Ranchini

Biology Faculty Articles

Homologous env sequences from 17 human T-leukemia/lymphotropic virus type I (HTLV-I) strains from throughout the world and from 25 simian T-leukemia/lymphotropic virus type I (STLV-I) strains from 12 simian species in Asia and Africa were analyzed in a phylogenetic context as an approach to resolving the natural history of these related retroviruses. STLV-I exhibited greater overall sequence variation between strains (1 to 18% compared with 0 to 9% for HTLV-I), supporting the simian origin of the modern viruses in all species. Three HTLV-I phylogenetic clusters or clades (cosmopolitan, Zaire, and Melanesia) were resolved with phenetic, parsimony, and likelihood analytical procedures. …

Jefferson Alumni Bulletin – Volume Xliii, Number 3, Spring 1994

The Bulletin (formerly the Jefferson Medical College Alumni Bulletin)

Jefferson Alumni Bulletin – Volume XLIII, Number 3, Spring 1994

Anesthesiology is never at rest, page 2

The medical college’s past ten years and its future, page 8

New health care climate, page 13

Student life, page 15

Books, page 14

Albuterol may increase the effectiveness of Parkinson’s disease therapy, page 16

NIH Continues support for scleroderma research, page 16

Faculty number among area’s most cited scientists, page 16

On campus, page 16

Class notes, page 22

Amoebic Appendicitis--A Rare Entity, Raheem Ahmed, Hizbullah Shaikh, Muhammad Siddiqui, M Ahmed

Department of Pathology and Laboratory Medicine

Appendectomy is the most common surgical procedure performed in routine general surgical practice. However, not all the appendices removed, are submitted for histopathological examination in this part of the world. We reviewed 1400 appendices received by our department from within our hospital and from outside the hospital. Of these 13 cases were reported as amoebic appendicitis. These patients did not have any different clinical presentation from the patients who were reported as acute appendicitis without amoebae. Microscopically these appendices had minimal neutrophil polymorph infiltration accompanied by tissue necrosis and amoebic trophozoites within the appendiceal wall. After histological diagnosis, different tests …

Tyrosine As A Countermeasure To Performance Decrement During Sleep Loss, Douglas A. Wiegmann, David F. Neri, Robert R. Stanny, Scott Shappell, Andrew M. Mccardie, David L. Mckay

Publications

The fatigue and cognitive performance deficits associated with sleep loss and stress have motivated the search for effective nonpharmacological countermeasures. The purpose of the present study was to examine the potential behavioral effects of tyrosine, an amino-acid presursor to dopamine and norepinephrine, during an episode of continuous nighttime work involving one night of sleep loss.

Elevated Expression Of Type Vii Collagen In The Skin Of Patients With Systemic Sclerosis. Regulation By Transforming Growth Factor-Beta., Lidia Rudnicka, John Varga, Angela M. Christiano, Renato V. Iozzo, Sergio A. Jimenez, Jouni Uitto

Department of Dermatology and Cutaneous Biology Faculty Papers

A hallmark of systemic sclerosis (SSc) is the development of tissue fibrosis. Excessive production of several connective tissue components normally present in the dermis, including type I, III, V, and VI collagens as well as fibronectin and proteoglycans, is a consistent finding in the skin of SSc patients. Type VII collagen is a major constituent of anchoring fibrils, present in the skin at the dermal-epidermal basement membrane zone. TGF-beta has been shown to upregulate the expression of the type VII collagen gene. In this study, we assessed the expression of type VII collagen and TGF-beta in the skin of patients …

The Identification And Reporting Of Physical Abuse By Physicians: A Review And Implications For Research, Jody E. Warner, David J. Hansen

Department of Psychology: Faculty Publications

Identification and reporting of possible cases of child physical abuse are critical precursors to intervention with maltreating families. Professionals from a variety of disciplines are mandated to report suspected cases of child maltreatment. Unfortunately, not all physically abused children are identified or reported. This paper reviews the literature that has examined factors that may influence the identification and reporting of physical abuse by physicians. The literature review is preceded by an overview of the multistep, multibehavior process of identification and reporting. The factors that may influence identification and reporting are discussed according to their association with the case, physician, or …

Extra-Legal Factors And Product Liability: The Influence Of Mock Jurors’ Demographic Characteristics And Intuitions About The Cause Of An Injury, Brian H. Bornstein, Michelle Rajki

Department of Psychology: Faculty Publications

Two experiments were performed to investigate the role of extra-legal factors in a simulated product liability trial. In cases where the factual evidence was identical, subjects’ liability judgments varied as a function of the case-specific factor of the alleged source of the plaintiff’s injury. In deciding cases differently depending on the alleged cause, subjects relied on intuitions about what injury sources are more or less likely to cause a certain kind of injury. Juror- specific factors also influenced subjects’ verdicts. There was no difference between students and non-students, but race and SES—factors that are often correlated with student status—did affect …

Information Interface - Volume 18, Issue 2 - March/April 1994, George Washington University, Himmelfarb Health Sciences Library

Information Interface (1976 - 2009)

News and information about Himmelfarb Health Sciences Library of interest to users.

Possible Roles Of Insulin And Insulin-Like Growth Factors In Rat Preimplantation Development: Investigation Of Gene Expression By Reverse Transcription-Polymerase Chain Reaction., X Zhang, G M Kidder, A J Watson, G A Schultz, D T Armstrong

Obstetrics & Gynaecology Publications

The sensitive mRNA phenotyping technique of reverse transcription-polymerase chain reaction was used to demonstrate that insulin receptor mRNA is present in rat embryos during the preimplantation period. In addition, mRNA encoding insulin-like growth factor (IGF) type I and type II receptors have also been detected in rat preimplantation embryos. IGF-I mRNA was not detected in preimplantation embryos but was found in oviducts and uteri of prepubertal and early pregnant rats. IGF-II mRNA was present in both embryos and in oviducts and uteri during the preimplantation period. These findings suggest that insulin and IGF-I could influence early embryo development in endocrine …

Conjoined Twins - A Review Of Literature With Two Case Studies, Naaeem Uz-Zafar Khan, Nadeem Ullah Khan, Khalid Matin, Adnan Zaidi

Department of Emergency Medicine

Two cases of conjoined twins have been reported. A review of literature is given.

Serum Alpha 1 Antitrypsin In Liver Diseases, Anjum Shahid, Anwar Ali Siddiqui, Sarwar J. Zuberi, M A. Waqar

Department of Biological & Biomedical Sciences

No abstract provided.

Clinical Occupational Bloodborne Exposure In A Dental School, Cesar D. Gonzalez, Ronald J. Pruhs, Elise Sampson

School of Dentistry Faculty Research and Publications

No abstract provided.

Dynamics Of Host Blood Effects In Glossina Morsitans Sspp. Infected With Trypanosoma Congolense And T. Brucei, Reardon O. Olubayo, Steve Mihok, Eli Munyoki, Leonard H. Otieno

All Peer-Reviewed Publications

The pattern of infection in Glossina morsitans morsitans and G. m. centralis membrane-fed on eland, buffalo or goat blood mixed with Trypanosoma congolense or T. brucei was studied from day 1 to day 10. Tsetse were initially permissive vectors, with most flies harbouring infections of 104-105 parasites on day 3. However, after a second blood meal on day 3, flies cleared many infections with G. m. morsitans clearing more infections than G. m. centralis. Infective feeds of goat blood consistently increased final infection rates by limiting the number of infections lost between days 3 and 6. In further experiments with …

The Amino-Terminal Functions Of The Simian Virus 40 Large T Antigen Are Required To Overcome Wild-Type P53-Mediated Growth Arrest Of Cells., Robin S. Quartin, Charles N. Cole, James M. Pipas, Arnold J. Levine

Dartmouth Scholarship

High levels of the p53 tumor suppressor protein can block progression through the cell cycle. A model system for the study of the mechanism of action of wild-type p53 is a cell line (T64-7B) derived from rat embryo fibroblasts transformed by activated ras and a temperature-sensitive murine p53 gene. At 37 to 39 degrees C, the murine p53 protein is in a mutant conformation and the cells actively divide, whereas at 32 degrees C, the protein has a wild-type conformation and the cells arrest in the G1 phase of the cell cycle. Wild-type simian virus 40 large T antigen and …

Malakoplakia Of The Uterus, H Shaikh, P E. Khan, N Faridi

Department of Pathology and Laboratory Medicine

No abstract provided.

Identification And Characterization Of A Human Herpesvirus 6 Gene Segment Capable Of Transactivating The Human Immunodeficiency Virus Type 1 Long Terminal Repeat In An Spl Binding Site-Dependent Manner, Jinhai Wang, Clinton J. Jones, Michael Norcross, Ernst Bohnlein, Abdur Razzaque

School of Veterinary and Biomedical Sciences: Faculty Publications

The human immunodeficiency virus type 1 (HIV-1) long terminal repeat (LTR) is transactivated by various extracellular signals and viral cofactors that include human herpesviruses. These transactivators are capable of transactivating the HIV-1 LTR through the transactivation response element, NF-KB, or other regulatory binding elements. Human herpesvirus 6 (HHV-6) is a potential cofactor of HIV-1. Here, we report that an HHV-6 gene segment, ZVH14, which can neoplastically transform NIH 3T3 and human keratinocytes, is capable of transactivating HIV-1 LTR chloramphenicol acetyltransferase constructs in an Spl binding site-dependent manner. Transactivation increased synergistically in the presence of multiple Spl sites and was dramatically …

Wild Medicine: Review Of Medicinal Wild Plants Of The Prairie By Kelly Kindscher, Kathleen H. Keeler

School of Biological Sciences: Faculty Publications

I am highly enthusiastic about this book as a reference work. It summarizes the literature on the medicinal uses for more than 103 prairie plants. For 43 species, Kindscher gives common, Indian, and scientific names; description; habitat; parts used; Indian uses; medical history; scientific research and cultivation; and information on about 100 of their relatives. Another 60 entries are condensed to 24 paragraphs. Full-page line drawings by William S. Whitney of the major species are generally excellent, and all 103 entries have distribution maps. The introduction discusses the region covered, with helpful maps. There's an index, a glossary and a …

Adolescent Health Care A Program Review And Descriptive Summary, Native American Management Services, Inc.

Native Health Database Full Text

This final report is presented in two parts. Part 1 is the description and results of the IHS HP/DP Adolescent Health Centers Grant Program review. Part 2 is the description of AI/AN adolescent health care in the IHS. Results The purpose of these grant demonstration projects was to address the specific health problems of AI/AN adolescents and emphasize health promotion and disease prevention with cultural relevance and sensitivity.

Roundtable Conference On Health Care Reform, The Health Security Act, And Indian Health Care: A Briefing Book, U.S. Indian Health Service

Native Health Database Full Text

This document contains information in the following sections: 1) legal history of Federal responsibility to provide care; 2) Indian health care delivery system; 3) health care reform and Indian health; and 4) analysis of Health Care Security Act proposal. This Briefing Book is provided to Roundtable participants to aid in their preparation for and reference during the conference. A final report will be issued by the Indian Health Service that will include the consensus statements developed by participants in this second Roundtable Conference on Health Care Reform.