Medicine and Health Sciences Commons^™

Association Of Epstein Barr Virus (Ebv) With Nasopharyngeal Carcinoma (Npc), S H. Shah, I N. Soomro, S Haroon, T Moatter

Department of Pathology and Laboratory Medicine

Aim:
To observe the frequency of nasopharyngeal carcinoma (NPC) and its association with Epstein Barr Virus (EBV) infection.
Setting:
This study included consecutive cases of nasopharyngeal carcinoma, which were diagnosed in the Department of Pathology at the Aga Khan University Hospital, Karachi in the period of two years (1996-97).
Methods:
These tumors were initially evaluated on H&E stained sections. The tumors showing evidence of keratinization were excluded from the study. The Epstein Barr Virus was detected with the help of Polymerase chain reaction in formalin fixed, paraffin embedded tissue sections.
Results:
During the study period, seventeen cases of nasopharyngeal carcinoma …

Pharmaceutical Marketplace Dynamics, Robin J. Strongin

National Health Policy Forum

This issue brief provides a profile of the pharmaceutical industry and explores topics such as competition, generics, intellectual property, research and development, pricing, and distribution. It also discusses government programs such as the Department of Veterans' Affairs federal supply schedule and Medicaid rebates.

Host-Derived Signals For Inducing Isoprenoid Gene Expression And Uses Thereof, Joseph Chappell, Marcos Lusso

Pharmaceutical Sciences Faculty Patents

Disclosed is a method for preparing a composition that is capable of activating the expression of a gene involved in the synthesis of an isoprenoid, the method involving: (a) contacting a plant cell with an elicitor under conditions that allow an elicitor-induced release of a compound that activates the synthesis of an isoprenoid; and (b) recovering a composition including the compound, wherein the compound is diffusible and has a molecular weight less than or equal to 10,000 daltons. Also disclosed is a substantially pure elicitor-induced composition, the composition being capable of activating a gene involved in the synthesis of a …

Novel Function Of Phosphoinositide 3-Kinase In T Cell Ca2+ Signaling. A Phosphatidylinositol 3,4,5-Trisphosphate-Mediated Ca2+ Entry Mechanism., A L Hsu, T T Ching, G Sen, D S Wang, Subbarao Bondada, K S Authi, C S Chen

Microbiology, Immunology, and Molecular Genetics Faculty Publications

This study presents evidence that phosphoinositide (PI) 3-kinase is involved in T cell Ca(2+) signaling via a phosphatidylinositol 3,4, 5-trisphosphate PI(3,4,5)P(3)-sensitive Ca(2+) entry pathway. First, exogenous PI(3,4,5)P(3) at concentrations close to its physiological levels induces Ca(2+) influx in T cells, whereas PI(3,4)P(2), PI(4, 5)P(2), and PI(3)P have no effect on [Ca(2+)](i). This Ca(2+) entry mechanism is cell type-specific as B cells and a number of cell lines examined do not respond to PI(3,4,5)P(3) stimulation. Second, inhibition of PI 3-kinase by wortmannin and by overexpression of the dominant negative inhibitor Deltap85 suppresses anti-CD3-induced Ca(2+) response, which could be reversed by subsequent …

Efflux Of Osmolyte Amino Acids During Isovolumic Regulation In Hippocampal Slices, Rodrigo Franco, Octavio Quesada, Herminia Pasantes-Morales

School of Veterinary and Biomedical Sciences: Faculty Publications

The efflux of potassium (K⁺) and amino acids from hippocampal slices was measured after sudden exposure to 10% (270 mOsm), 25% (225 mOsm) or 50% (150 mOsm) hyposmotic solutions or after gradual decrease (22.5 mOsm/min) in external osmolarity. In slices suddenly exposed to 50% hyposmotic solutions, swelling was followed by partial (74%) cell volume recovery, suggesting regulatory volume decrease (RVD). With gradual hyposmotic changes, no increase in cell water content was observed even when the solution at the end of the experiment was 50% hyposmotic, showing the occurrence of isovolumic regulation (IVR). The gradual decrease in osmolarity elicited …

Effect Of Receptor-Selective Retinoids On Growth And Differentiation Pathways In Mouse Melanoma Cells, Sejal H. Desai, Goran Boskovic, Linda L. Eastham, Marcia Dawson, Richard M. Niles

Biochemistry and Microbiology

Treatment of B16 mouse melanoma cells with all-trans-retinoic acid (ATRA) results in inhibition of cell proliferation and induction of differentiation. Accompanying these events is an induction of retinoic acid receptor β (RARβ) expression, an increase in protein kinase Cα (PKCα) expression, and enhanced activator protein-1 (AP-1) transcriptional activity. These cells express nuclear RARα and RARγ and nuclear retinoid X receptors (RXR) α and β constitutively. We tested the ability of receptor-selective retinoids to induce the biochemical changes found in ATRA-treated melanoma cells and also tested their effectiveness in decreasing anchorage-dependent and -independent growth. The RXR-selective ligand (2E,4E)-6-(5,6,7,8-tetrahydro-3,5,5,8,8-pentamethyl-2-naphthalenyl)-3,7-dimethyl-2,4,6-octatrienoic acid (SR11246) was …

Ada News - 05/15/2000, American Dental Association, Publishing Division

ADA News

Established in 1970 as the official newspaper of the American Dental Association, the ADA News serves practicing dentists and others allied to the dental profession in the U.S. and internationally. It is the No. 1 source of news and information about the many benefits and services the ADA delivers to members daily as well as timely information on scientific, social, political and economic developments affecting dentistry and health care.

Want To Ride The Dot.Com Gravy Train, Rick Burton

Sport Management - All Scholarship

No abstract provided.

2000 Commencement For College Of Health Professions

Jefferson College of Nursing Commencements

No abstract provided.

Ua66/4/2 Ceremony Of Capping & Pinning, Wku Dental Hygiene

WKU Administration Documents

Program from capping & pinning ceremony.

Could Humans Be Transfused With Pig And Cow Blood?, Donalee Moulton

Data and Test Instruments

Today, hemophilia patients who have developed antibodies against human factor VIII use factor VIII derived from pigs' blood. "There is no evidence of disease being transferred," said Dr. [VivianMcAlister]. "However, great caution is required to make sure we know all the potential risks are controlled before transfusions of cells are attempted."

Low Fasting Plasma Glucose Level As A Predictor Of Cardiovascular Disease And All-Cause Mortality, Ming Wei, Larry W. Gibbons, Tedd L. Mitchell, James B. Kampert, Michael P. Stern, Steven N. Blair

Faculty Publications

Background - Although medical textbooks usually classify fasting plasma glucose <70 or 80 mg/dL (<3.89 or 4.44 mmol/L) as abnormal, the prognosis for patients with low fasting plasma glucose is unclear.

Methods and Results - We conducted prospective cohort studies among 40,069 men and women to investigate the association between fasting plasma glucose levels and cardiovascular disease and all-cause mortality. We documented a U-shaped relation between fasting plasma glucose and mortality. In addition to diabetes and impaired fasting glucose levels, low fasting plasma glucose levels were also associated with high morality. After multivariate adjustment for age, sex, study population, ethnicity, current smoking status, high blood pressure, total cholesterol, body mass index, triglycerides, history of cardiovascular disease and cancer, and a family …

Gene Inhibition Using Genetic Suppressor Elements In Escherichia Coli Jm109de3 Cells, Ronald Chuke

Masters Theses & Specialist Projects

Genetic suppressor elements (GSEs) are short biologically active gene fragments that encode inhibitory antisense RNAs or truncated proteins that function as negative dominants. GSEs can be constructed from a single gene or isolated from a multigene library consisting of short random fragments of the target gene or genes. The goal of this study was to determine if gene inhibition in bacterial cells could be achieved by isolating GSEs from a genomic library. Additionally, if it was possible to use GSEs to inhibit gene function in E. coli JM109DE3 cells, could this method be used to locate and determine the function …

Density And Age Distributions Of Trees In Upland And Lowland Oldfields 60 Years After Abandonment: The Role Of Dispersal, Virginia Mcdaniel

Masters Theses & Specialist Projects

I attempted to determine whether the patterns of seedling establishment described in the literature for forests and recently abandoned oldfields are maintained in fields sixty years after abandonment. To determine this distribution pattern, as well as to compare patterns of age and density distribution in upland and lowland oldfields, I conducted research at Mammoth Cave National Park. I studied three species, the seeds of which are dispersed by three different modes: Juniperus virginiana, a predominantly bird-dispersed species; Acer rubrum, a predominantly winddispersed species; and Fagus grandifolia, a predominantly mammal-dispersed species. The establishment patterns observed by others in recently abandoned oldfields …

Wellness Center Healthletter- May 2000, Nova Southeastern University

Wellness Center Healthletter

No abstract provided.

Information Interface - Volume 28, Issue 3 - May/June 2000, George Washington University, Himmelfarb Health Sciences Library

Information Interface (1976 - 2009)

News and information about Himmelfarb Health Sciences Library of interest to users.

Teaching The Ethics Of Biology, David Harris Phd, Carol K. Johansen

Faculty Publications

Discusses the basic principles of ethics and ethical decision making as applied to biology. Ethical issues associated with biology; Theoretical basis of ethical decision-making; Models of ethical decision-making; Social implications of scientific experimentation and discovery.

Regulation Of Acetylated Low Density Lipoprotein Uptake In Macrophages By Pertussis Toxin-Sensitive G Proteins, Stewart C. Whitman, Alan Daugherty, Steven R. Post

Gill Heart & Vascular Institute Faculty Publications

Class A scavenger receptors (SR-A) mediate the uptake of modified low density lipoprotein (LDL) by macrophages. Although not typically associated with the activation of intracellular signaling cascades, results with peritoneal macrophages indicate that the SR-A ligand acetylated LDL (AcLDL) promotes activation of cytosolic kinases and phospholipases. These signaling responses were blocked by the treatment of cells with pertussis toxin (PTX) indicating that SR-A activates G_i/o-linked signaling pathways. The functional significance of SR-A-mediated G_i/o activation is not clear. In this study, we investigated the potential role of G_i/o activation in regulating SR-A-mediated lipoprotein uptake. Treatment of mouse …

Ada News - 05/01/2000, American Dental Association, Publishing Division

ADA News

Established in 1970 as the official newspaper of the American Dental Association, the ADA News serves practicing dentists and others allied to the dental profession in the U.S. and internationally. It is the No. 1 source of news and information about the many benefits and services the ADA delivers to members daily as well as timely information on scientific, social, political and economic developments affecting dentistry and health care.

How Children And Adolescents Relate To Nature, Patricia Nevers

Center for the Study of Ethics in Society Papers

Paper presented at the Center for the Study of Ethics in Society at Western Michigan University, September 21,1999.

Electrically Mediated Plasmid Dna Delivery To Hepatocellular Carcinomas In Vivo, L. Heller, M. J. Jaroszeski, D. Coppola, C. Pottinger, R. Gilbert, Richard Heller

Bioelectrics Publications

Gene therapy by direct delivery of plasmid DNA has several advantages over viral gene transfer, but plasmid delivery is less efficient. In vivo electroporation has been used to enhance delivery of chemotherapeutic agents to tumors in both animal and human studies. Recently, this delivery technique has been extended to large molecules such as plasmid DNA. Here, the successful delivery of plasmids encoding reporter genes to rat hepatocellular carcinomas by in vivo electroporation is demonstrated.

Center-Based Prevalence Of Anxiety And Depression In Women Of The Northern Areas Of Pakistan, Sunita Dodani, Rukhsana Wamiq Zuberi

Department for Educational Development

Objective: To estimate the prevalence of anxiety and depression in women of the Northern areas. Methods: A cross-sectional center-based study was conducted at Singal Medical Center (SNIC), Gilgit, in the Northern Areas of Pakistan, using the Hospital Anxiety and Depression Scale (HADS).

Results: One hundred and twenty women, between the ages of 16 and 60, attended the SMC over a two month period and were included in the study. Using HADS, it was found that 50% of the women had anxiety and/or depression; 25% suffered only from anxiety, 8% from depression and 17% had features of both.

Conclusion: This study …

Decision Support Methods In Diabetic Patient Management By Insulin Administration Neural Network Vs. Induction Methods For Knowledge Classification, B. V. Ambrosiadou, S. Vadera, Venky Shankaraman, D. Goulis, G. Gogou

Research Collection School Of Computing and Information Systems

Diabetes mellitus is now recognised as a major worldwide public health problem. At present, about 100 million people are registered as diabetic patients. Many clinical, social and economic problems occur as a consequence of insulin-dependent diabetes. Treatment attempts to prevent or delay complications by applying ‘optimal’ glycaemic control. Therefore, there is a continuous need for effective monitoring of the patient. Given the popularity of decision tree learning algorithms as well as neural networks for knowledge classification which is further used for decision support, this paper examines their relative merits by applying one algorithm from each family on a medical problem; …

Unusual Polymorphisms In Human Immunodeficiency Virus Type 1 Associated With Nonprogressive Infection, Louis Alexander, Emma Weiskopf, Thomas C. Greenough, Nathan C. Gaddis, Marcy C. Auerbach, Michael H. Malim, Stephen J. O'Brien, Bruce D. Walker, John L. Sullivan, Ronald C. Desrosiers

Biology Faculty Articles

Factors accounting for long-term nonprogression may include infection with an attenuated strain of human immunodeficiency virus type 1 (HIV-1), genetic polymorphisms in the host, and virus-specific immune responses. In this study, we examined eight individuals with nonprogressing or slowly progressing HIV-1 infection, none of whom were homozygous for host-specific polymorphisms (CCR5-Δ32, CCR2-64I, and SDF-1-3^'A) which have been associated with slower disease progression. HIV-1 was recovered from seven of the eight, and recovered virus was used for sequencing the full-length HIV-1 genome; full-length HIV-1 genome sequences from the eighth were determined following amplification of viral …

Large-Scale Synthesis And Functional Elements For The Antimicrobial Activity Of Defensins, Periathamby A. Raj, K. J. Antonyraj, T. Karunakaran

School of Dentistry Faculty Research and Publications

Human neutrophil defensins, and their analogues incorporating anionic, hydrophobic or cationic residues at the N- and C-termini, were synthesized by solid-phase procedures. The synthetic defensins were examined for their microbicidal activity against Candida albicans, two Gram-negative bacteria (Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis) and two Gram-positive bacteria (Streptococcus gordonii and Streptococcus mutans). The human neutrophil peptide 1 (HNP1) and HNP2 were found to be potent candidacidal agents. HNP3, which differs by one amino acid at the N-terminus of its sequence, was totally inactive. The Gram-negative bacteria A. actinomycetemcomitans and P. gingivalis and the Gram-positive bacteria S. gordonii and S. mutans were …

Parenting A Second Time Around: An Ethnography Of African American Grandmothers Parenting Grandchildren Due To Parental Cocaine Abuse, Kristin Haglund

College of Nursing Faculty Research and Publications

This study describes a group of six African American grandmothers parenting their grandchildren secondary to cocaine abuse on the part of the parents. It explores the manner in which such parenting affected the grandmothers’ health. Data for this ethnography design were collected through participant observation, field notes, taped interviews, and supplementary data sources. The identification of cultural themes evolved from domain and taxonomic analyses. The themes—parenting a second time around, sacrifice, and God’s presence in daily life—expressed aspects of the grandmothers’ culture. The effects on health varied from none to exacerbation of chronic illnesses. The study results, and its picture …

Relationship Between Scholastic And Health Behaviors And Reading Level In Adolescent Females, Steven C. Matson, Kristin Haglund

College of Nursing Faculty Research and Publications

This was a study of 102 adolescent females, 12-20 years of age, presenting to a central city clinic for medical care. Participants completed an oral questionnaire that included demographics, and questions regarding scholastic history, sexual behavior, and substance use. Each subject completed the Accuracy Level Test (ALT), a reading test. The subject's reading test grade level was subtracted from her appropriate grade in school to give a reading delay level (RDL). The mean reading grade level for all subjects was 6.7 ±2.6 and the average reading delay was 4.5 ±2.5 grades. Poor school attenders had greater reading delays (5.8 ±3.4 …

Central Nervous System Lymphomas: A Histologic And Immunophenotypic Analysis, Suhail Muzaffar, M. Shahid Siddiqui, Irshad Nabi Soomro, Shahid Pervez, Naila Kayani Naila Kayani, Sheema H. Hasan Sheema H. Hasan Sheema H. Hasan, Lmran Siddiqui Lmran Siddiqui

Department of Pathology and Laboratory Medicine

Objective: To observe the spectrum of non-Hodkin’s lymphomas involving the central nervous system including morphological subtypes and immunophenotypic status.

Setting: Retrospective analysis of eleven years (1986 to 1996) data from surgical pathology files of Department of Pathology.

Results: orty-three cases of non-Hodgkin’s lymphomas were diagnosed during the period of eleven years (from 1986 to 1996), all of which were diffuse types. A total of 1177 Central Nervous CNS biopsies were examined, out of which 937 cases were diagnosed as CNS neoplasms, the remaining were non-neoplastic in nature. Among 937 CNS neoplasms, 43 cases (4.6%) were reported as non-Hodgkin’s lymphomas. As …

Ua66/14/1 Advisory Board Meeting, Wku Nursing

WKU Administration Documents

Meeting minutes of the WKU Nursing Advisory Board/

Ceftriaxone-Resistant Salmonella Infection Acquired By A Child From Cattle, Paul D. Fey, Thomas J. Safranek, Mark E. Rupp, Eileen F. Dunne, Efrain Ribot, Peter C. Iwen, Patricia A. Bradford, Frederick J. Angulo, Steven H. Hinrichs

Zoonotics and Wildlife Disease: Publications

Background
The emergence of resistance to antimicrobial agents within the salmonellae is a worldwide problem that has been associated with the use of antibiotics in livestock. Resistance to ceftriaxone and the fluoroquinolones, which are used to treat invasive salmonella infections, is rare in the United States. We analyzed the molecular characteristics of a ceftriaxone-resistant strain of Salmonella enterica serotype typhimurium isolated from a 12-year-old boy with fever, abdominal pain, and diarrhea.
Methods
We used pulsed-field gel electrophoresis and analysis of plasmids and β-lactamases to compare the ceftriaxone-resistant S. enterica serotype typhimurium from the child with four isolates of this strain …