Medicine and Health Sciences Commons^™

Design And Development Of A Plasmid Vector For Protein Expression And Purification, Mahima Grover, Craig Sweet, David H. Thompson

The Summer Undergraduate Research Fellowship (SURF) Symposium

Production and isolation of proteins are difficult, costly and time-consuming processes. The aim of this project is for the development of plasmids, which allow for streamlined production and isolation of proteins. To allow for modular insertion of varying segments of DNA we are using ‘recursive directional ligation by plasmid reconstruction’. This technique uses type II restriction endonucleases, which cut downstream from their recognition site allowing multiple insertions without losing a restriction site. Using this process, we can ligate multiple DNA sequences together and express them to be able to construct a scar less fusion protein. In order to accomplish this, …

The Role Of Osteocyte Estrogen Receptor Beta (Erβ) In Regulating The Skeletal Response To Mechanical Loading, Julia P. Townsend, Russell P. Main

The Summer Undergraduate Research Fellowship (SURF) Symposium

Estrogen’s biological functions are mediated by estrogen binding to estrogen receptors (ER). Understanding what role both ERα and ERβ have in bone maintenance and formation can contribute to possible treatment of osteoporosis. This study examined osteocyte specific deletion of ERβ in mice. The cross of ERβ-floxed mice with DMP1-8kb-Cre mice provided both experimental knockout mice as well as littermate control mice. At 24 weeks of age the left tibiae of all mice were mechanically loaded five days per week for two weeks to induce bone formation. Analysis of cortical bone was conducted using microcomputed tomography (microCT) to measure load-induced changes …

Pathway By Which Vagus Nerve Stimulation Of B Fibers Affects Heart Rate, Kelsey Wasilczuk, Matthew Ward, Pedro Irazoqui

The Summer Undergraduate Research Fellowship (SURF) Symposium

Heart failure (HF) affects over 5 million adults in the United States. Many HF patients have a high resting heart rate, which is correlated with a high mortality rate. In recent years, vagus nerve stimulation (VNS) has become an increasingly researched therapy to reduce the resting heart rate of HF patients. However, current dosage given during VNS is increased incrementally at the doctor’s office until side effects present themselves in a patient. In addition, the means by which the therapy works is not completely understood. To better understand the therapy’s mechanisms, the right cervical vagus nerve of several Long Evans …

Nanobubbles Provide Theranostic Relief To Cancer Hypoxia, Christopher M. Long, Pushpak N. Bhandari, Joseph Irudayaraj

The Summer Undergraduate Research Fellowship (SURF) Symposium

Hypoxia is a common motif among tumors, contributing to metastasis, angiogenesis, cellular epigenetic abnormality, and resistance to cancer therapy. Hypoxia also plays a pivotal role in oncological studies, where it can be used as a principal target for new anti-cancer therapeutic methods. Oxygen nanobubbles were designed in an effort to target the hypoxic tumor regions, thus interrupting the hypoxia-inducible factor-1α (HIF-1α) regulatory pathway and inhibiting tumor progression. At less than 100nm, oxygen nanobubbles act as a vehicle for site-specific oxygen delivery, while also serving as an ultrasound contrast agent for advanced imaging purposes. Through in vitro and in vivo studies, …

Leveraging Smart Infusion Pump Data For Workflow, Patient Care And Usability Improvement In Human Factors, Yan Ni Ding, Denny Yu, Poching Delaurentis, Kang-Yu Hsu, Joon Hong Kim

The Summer Undergraduate Research Fellowship (SURF) Symposium

Infusion pumps are medical devices that deliver fluids like medication, and nutrients in a precise, timely, and controlled manner that is critical to patient care. It is widely used in clinical settings especially in hospitals, nursing homes and sometimes at home. Smart infusion pumps technology are supposed to be reduce nurses’ workload, but due to the recurring number of alarms which disrupt the workflow of the infusion process, most nurses prefer to use the traditional infusion pumps or work-around the safety features of the smart pumps. Thus, the aim of this research is to leverage Smart Infusion Pump data to …

Analyzing Sports Training Data With Machine Learning Techniques, Rehana Mahfuz, Zeinab Mourad, Aly El Gamal

The Summer Undergraduate Research Fellowship (SURF) Symposium

In the sports industry, there has not been enough effort in analyzing the personalized monitoring data of athletes collected during training sessions. This research is an attempt to find meaningful patterns in the Purdue Women’s Soccer training data that could help the coach design more efficient training sessions. We are specifically interested in studying this problem as an unsupervised learning problem. Our initial attempt is to cluster the players as well as drills into groups using k-means, c-means and spectral clustering algorithms, combined with feature transformation and reduction steps. These basic algorithms serve as a benchmark to measure performance improvements …

Characterization Of Left-Ventricular Thrombus Formation Using High Frequency Ultrasound, Kelsey A. Bullens, Arvin H. Soepriatna, Pavlos P. Vlachos, Craig J. Goergen

The Summer Undergraduate Research Fellowship (SURF) Symposium

Heart failure is a leading cause of death in the United States, and cardiac thrombus, a common morbidity associated with heart failure, significantly increases a patient’s risk of embolic events. The objective of this project is to characterize left-ventricular (LV) thrombus development using high frequency ultrasound imaging in a murine model. C57BL/6J wild-type mice (n=6) were injected intraperitoneally with iron dextran five times a week for six weeks to increase oxidative stress in the heart. Granulocyte-colony stimulating factor (G-CSF) was subcutaneously injected daily during the second week to initiate stem cell migration and stimulate endothelial cell activation, thus increasing the …

Ball Pressure Correlations With Peak Impact Force And The Potential For Cumulative Mtbi When Heading A Soccer Ball, Nicolas Leiva, Daniel Y. Shyu, Josh Auger, Eric Nauman

The Summer Undergraduate Research Fellowship (SURF) Symposium

Soccer is a unique sport in which athletes use their heads as tools for gameplay, which may ultimately cause cumulative traumatic brain injuries. Due to rising popularity of soccer in the United States alongside the increased occurrence of CTE and mTBI in other contact sports, there is a growing concern over how to keep the repetitive forces caused by heading, as low as possible. Different variables that can affect the peak force felt when heading a soccer ball can be simulated and compared with in-game data, however, this has never been properly tested before. In the present study two size …

Cartilage Engineering: Optimization Of Media For Chondrogenic Differentiation In Vitro, Evan Surma, Sherry L. Harbin, Hongji Zhang, Stacy Halum

The Summer Undergraduate Research Fellowship (SURF) Symposium

Lower back pain from intervertebral disc injury affects around 84% of the population at some point in their life, which at its worst may cause total immobilization. This pain can only be temporarily relieved by spinal fusion or intervertebral disc replacement; however, both of these cause loss of natural motion in patients by removing damaged fibrocartilage discs. While these techniques help mitigate pain briefly, no permanent solution exists currently to both relieve pain and preserve natural motion. My work may be a solution by eventually providing patient-specific implants that resemble native tissue in the regeneration process that could be absorbed …

Mechanical Reliability Of Implantable Polyimide-Based Magnetic Microactuators For Biofouling Removal, Christian G. Figueroa-Espada, Qi Yang, Hyowon Lee

The Summer Undergraduate Research Fellowship (SURF) Symposium

Hydrocephalus is a neurological disorder that typically requires a long-term implantation of a shunt system to manage its symptoms. These shunt systems are notorious for their extremely high failure rate. More than 40% of all implanted shunt systems fail within the first year of implantation. On average, 85% of all hydrocephalus patients with shunt systems undergo at least two shunt-revision surgeries within 10 years of implantation. A large portion of this high failure rate can be attributed to biofouling-related obstructions and infections. Previously, we developed flexible polyimide-based magnetic microactuators to remove obstructions formed on hydrocephalus shunts. To test the long-term …

Development Of Standard Criteria To Evaluate The Effectiveness Of Helmets At Decreasing The Risk Of Concussions, Daniel Y. Shyu, Goutham N. Sankaran, Kevin G. Mciver, Nicolas Leiva, Eric A. Nauman

The Summer Undergraduate Research Fellowship (SURF) Symposium

In many sports, such as American football, accumulations of mild traumatic brain injuries have been suggested as a possible link to neurodegeneration and future mental disorders. With head impacts occurring at all levels of competition and in different sports, it is critical to develop an accurate method for quantifying the effects of head impacts and determining the efficacy of helmets. This study examines the derivation of different dimensionless numbers and ascertains the critical factors needed to predict the effects of head impacts, specifically the resulting accelerations from an impact. Given a known force of impact, parameters such as peak translation …

Assembly Of Nucleic Acid-Based Nanoparticles By Gas-Liquid Segmented Flow Microfluidics, Matthew L. Capek, Ross Verheul, David H. Thompson

The Summer Undergraduate Research Fellowship (SURF) Symposium

The development of novel and efficient mixing methods is important for optimizing the efficiency of many biological and chemical processes. Tuning the physical and performance properties of nucleic acid-based nanoparticles is one such example known to be strongly affected by mixing efficiency. The characteristics of DNA nanoparticles (such as size, polydispersity, ζ-potential, and gel shift) are important to ensure their therapeutic potency, and new methods to optimize these characteristics are of significant importance to achieve the highest efficacy. In the present study, a simple segmented flow microfluidics system has been developed to augment mixing of pDNA/bPEI nanoparticles. This DNA and …

Research On Problem Gambling Websites, Chang Boon Patrick Lee

International Conference on Gambling & Risk Taking

This is a continuation of a series of studies on problem gambling websites. It has been noted in prior studies that one avenue problem gamblers might look for help was to go online to perform a web search. Online help is practical as help can be located immediately and this can help to ease the gamblers’ concerns that they are left alone to handle their own problems. While there are some anecdotal evidences regarding the success of problem gambling websites, there have been few empirical studies that relate directly to the perceptions and usefulness of the websites for the problem …

An Empirical Evaluation Of The Clubsnsw (Australia) Multi-Venue Self-Exclusion Program, Dylan A. Pickering, Alexander Blaszczynski, Sally Gainsbury, Rowan Cameron

International Conference on Gambling & Risk Taking

Few empirical studies have evaluated the effectiveness of self-exclusion programs. Research is required to identify factors contributing to decisions to enter and/or breach self-exclusion orders, or to self-excluded gamblers to seek additional treatment. Clinical characteristics of self-excluders remain relatively unknown in addition to factors that differentiate gamblers who do or do not breach. Limitations of current programs are that gamblers can self-exclude from only one or a limited number of venues. The Multi-venue Self-exclusion program, developed and implemented by ClubsNSW (Australia), is a centralised web-based system designed to help problem gamblers self exclude from up to 35 venues at a …

Mandatory Pre-Commitment Loss Limits: Six Years’ Experience Shows It Works, Bjorn H. Hoffmann

International Conference on Gambling & Risk Taking

The Norwegian state owned company Norsk Tipping is a pioneer in the gambling indusrty with its secure player identification and mandatory registered play across all channels. Knowing who your player is and having a strong link between the player and his consumption of games across all channels (land based, EGM, web and mobile) is an important premise for having a credible responsible gaming platform.

Norsk Tipping introduced in 2009 EGM’s with both pre commitment tools and maximum loss limits. In 2014 we introduced our Internet casino with probably the best responsible gambling platform in the world, including mandatory loss limits. …

Best Practices For Self-Exclusion Reinstatement And Renewal, Alex Price

International Conference on Gambling & Risk Taking

While many studies have examined self-exclusion few have focused on the processes through which gamblers return at the end of their agreements. In 2014, the RGC Centre for the Advancement of Best Practices examined voluntary self-exclusion reinstatement and renewal in an effort to develop evidence-informed best-practices for both land-based and online gambling operations. The presentation outlines the findings of the study and the recommended best practices for reintegration and ban renewal.

The study examined a range of practices around the world. In the end the study recommended an active reinstatement process in which all participants are required to apply to …

Monitoring Gambling Impacts In Massachusetts: Honing Strategies And Preventing Harm, Rachel A. Volberg, Amanda Houpt

International Conference on Gambling & Risk Taking

In November 2011, an Act Establishing Expanded Gaming in the Commonwealth of Massachusetts was signed into law (Chapter 194 of the Acts of 2011). This legislation permits casinos and slot parlors in Massachusetts under the regulatory auspices of the Massachusetts Gaming Commission (MGC). Section 71 of the Expanded Gaming Act requires the MGC to establish an annual research agenda to assist in understanding the effects of casino gambling in Massachusetts and in minimizing the negative impacts. Research activities launched thus far include a comprehensive study of the social and economic impacts of casino gambling in Massachusetts (SEIGMA), a cohort study …

A Public Health-Based Approach To German Gaming Regulation, Knut Walter, Gerhard Buehringer

International Conference on Gambling & Risk Taking

The current situation of the German gambling market can be characterized by an unrelated framework of different legal systems, different regulatory authorities and different control principles, and has led to (1) an increasing number of court decisions and information requests by European Commission questioning parts of that regulatory system, (2) public tolerance of illicit gambling, and consequently (3) a lack of consumer protection.

Taking RENO Model 1.0 and 2.0 seriously, a new and comprehensive regulatory framework based on common consumer protection standards for all types of gambling – land-based and online, state-owned and private operated – has been developed and …

A Behavioural Account Of Mobile Gambling, Richard James, Richard Tunney, Claire O'Malley

International Conference on Gambling & Risk Taking

Mobile gambling is an emerging market in which there is evidence that some gamblers are introduced to gambling through their mobile device, and that mobile gambling does not ‘cannibalise’ participation with other forms of gambling. There is a concern that mobile gamblers face distinct risks from other forms of gambling, particularly for harmful behaviours. This paper presentation outlines a behavioural account of mobile gambling that combines both the learned characteristics of problem gambling with how individuals interact with their mobile devices. This hypothesizes that the extended gaps between plays or sessions potentially attracts extended play, even in the face of …

Ready, Set, And Go Back: The Role Of The Judiciary In Brazil’S Bingo Ban, Luiza Jobim Llm, Toni Williams

International Conference on Gambling & Risk Taking

Brazil, the host of the 2014 World Cup and the 2016 Olympics, is known for strong competitive traditions in sports and games. It is also one of only three G-20 countries (together with Saudi Arabia and Indonesia) that currently bans non-state provision of gambling products. Bingo was a notable exception to this prohibition, after enabling legislation was enacted in 1993, with the intention that proceeds would help fund national sports development. The game quickly became very popular but there were persistent questions about, and contestations over, the capacity of the regulatory framework to control the dual risks of exploitation of …

Problem Gambling Prevalence And Risk Factors Among Older Adults, Nigel E. Turner Ph.D., John Mccready, Robert Mann Ph.D., Mark Van Der Maas, Hayley Hamilton, Tracy Schrans

International Conference on Gambling & Risk Taking

Reports on the results of a study that interview older adults at casinos and racetrack slot machine venues (racinos) in Ontario. The study used an intercept survey employing cluster sampling methods to obtain a random sample of 2,103 Ontario older adults (aged 55 and older) gambling at seven sites across the province. Older adults exiting the gaming floor were randomly selected to be invited to participate in an interview / survey, which required about 15-20 minutes of their time. Participants provided information about their own gambling behavior and gambling-related problems, attitudes toward gambling, gambling and gambling problems in their community, …

Profiling At-Risk Gamblers, Tapio Jaakkola, Kari Vuorinen, Mirka Smolej

International Conference on Gambling & Risk Taking

The majority of gambling research has been concentrating on problem gambling and on the association between gambling behavior, game design and gambling harm. Much less attention has been directed at recreational gambling or on players that do not suffer from any gambling related problems. However, when it comes to preventing gambling harm we should also target those at risk as well those who are gambling moderately.

As a part of a project on prevention of adult gambling harm we did a study of at-risk gamblers in order to recognize and to define the socio-economic background of our target group. Prior …

Responsible Consumption Of Gambling: Results From A Survey Of Experts, Nerilee Hing, Alex M.T. Russell

International Conference on Gambling & Risk Taking

Consumers are now advised to “gamble responsibly” and to adopt a range of responsible gambling (RG) consumption strategies, such as setting time and expenditure limits, taking frequent breaks, and balancing gambling with other activities. However, it is unclear whether RG consumption strategies are aimed at non-problem and low-risk gamblers as a harm prevention strategy, higher-risk gamblers as a harm minimization strategy, or problem gamblers as a means to address their gambling problem. Indeed, conceptual developments in RG consumption are in their infancy and agreed definitions, principles, goals and behaviors are lacking.

This paper presents findings from an expert survey conducted …

Measuring Market Saturation In The U.S. Casino Industry: An Analytical And Empirical Analysis, Clyde W. Barrow, Alan P. Meister, David R. Borges

International Conference on Gambling & Risk Taking

The national and regional economies in the U.S. remain on a slow growth trajectory, while the casino gaming industry has seen a rapid and ongoing expansion. Consequently, states, Native American tribes, and gaming operators have increasingly shifted their attention from gaming expansion to the problems of regional competition, cannibalization, market maturation, and market saturation. The question of “market saturation” has become a salient point of public policy debate and a topic that is now frequently raised in the industry and media. This paper analyzes the concept of saturation in the context of casino gaming markets and compares several metrics for …

Gambling Warning Messages: The Impact Of Winning And Losing On Message Reception Across A Gambling Episode, Rory A. Pfund, Meredith K. Ginley, Samuel C. Peter, James P. Whelan, Andrew W. Meyers

International Conference on Gambling & Risk Taking

Warning messages prevent and modify risk-taking behaviors. When controlling the outcome of each wager, studies suggest such messages can increase a player’s knowledge about gambling-specific risks, modify their gambling-related cognitive distortions, and even change play. The present study takes the next step by asking if the reception of the warning message is influenced by the player’s experience of winning or losing.

In a laboratory study, participants were randomly assigned to a winning or losing gambling experience where they either viewed periodic warning messages or not. Using a mixed model analysis, the influence of the warning messages was related to players’ …

Gambling-Related Problems Of Self-Excluders In Swiss Casinos, Suzanne Lischer

International Conference on Gambling & Risk Taking

No abstract provided.

Interventions For Problem Gamblers In The Correctional System, Nigel E. Turner Ph.D., Flora Matheson

International Conference on Gambling & Risk Taking

The prevalence rates of problem gambling in the adult correctional population are 5 to 10 times higher than those found in the general population. The purpose of this study is to examine the type of interventions used to deal with this population. We conducted a combination of literature review and key informant study to examine the state of programming for problem gambling for clients who experience incarceration. The published literature on this topic is remarkably sparse. In fact only two peer reviewed published studies were identified that formally evaluated a treatment program for problem gambling for clients in these settings. …

Gambling Problems In The General Danish Population: Survey Evidence, Morten Lau, Don Ross

International Conference on Gambling & Risk Taking

We develop surveys of gambling problems in the general Danish population. We compare several popular survey instruments of gambling behavior and gambling propensity to assess if they differ in their classification of individuals. We also examine correlations with standard survey instruments for alcohol use, anxiety, depression and impulsiveness. A feature of our design is that nobody was excluded on the basis of their response to a “trigger,” “gateway” or “diagnostic item” question about previous gambling history, allowing us to check for sample selection bias. Our sample consists of 8,405 adult Danes, which is 12.8% of the sample frame of 65,592 …

Exploring The Utility Of An Extended Theory Of Planned Behaviour Framework For School-Based Gambling Prevention Programs, Renee A. St-Pierre Ph.D., Caroline E. Temcheff Ph.D., Jeffrey L. Derevensky Ph.D., Rina Gupta Ph.D.

International Conference on Gambling & Risk Taking

The theory of planned behaviour (TPB) and the concept of negative anticipated emotions (NAE) have attracted research attention in the formulation of effective preventive interventions. This approach has identified several key constructs of the TPB (i.e., intentions, attitudes, subjective norms, perceptions of behavioural control) and NAE as valid predictors of gambling behaviours and problems among young people (Martin et al., 2010, 2011; St-Pierre et al., 2015). However, no empirical investigation has utilized all of these constructs in the design or evaluation of an adolescent problem gambling preventive intervention. The current research aimed to assess the efficacy of targeting NAE and …

Gamblerization Of Post-Communist Society In Central Europe, Zdeněk Szczyrba, David Fiedor, Irena Smolová

International Conference on Gambling & Risk Taking

Countries of Central European region (Czech Republic, Slovakia, Poland and Hungary) joined a new stage of their development after 1989. In these countries, there was a building process of capitalism which went in parallel with far-reaching structural economic and social changes; such as the privatization of economies, de-industrialization but also gamblerization of society. Clarifying this term, gamblerization, it could be described as the process of a continuous penetration of gaming facilities in the area accompanied by an increased availability and accessibility of gaming machines in the society. It is important to say that this process was particularly allowed owing to …