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Full-Text Articles in Medicine and Health Sciences

The Short-Term Results Of Intensive Insulin Therapy In Preadolescent Children With Type-1 Diabetes, Ayşenur Ökten, Gülay Kaya, Mukaddes Doğan Kalyoncu, Gamze Çan Jan 2001

The Short-Term Results Of Intensive Insulin Therapy In Preadolescent Children With Type-1 Diabetes, Ayşenur Ökten, Gülay Kaya, Mukaddes Doğan Kalyoncu, Gamze Çan

Turkish Journal of Medical Sciences

Purpose: The aim of this study was to observe the effects of and compliance with an intensive insulin regimen in preadolescent children with type-1 diabetes. Patients and methods: Eleven insulin-dependent diabetic patients, five girls and six boys aged 8 - 11 years (mean 9 years 3 months) with a mean±SD diabetes duration of 2±1.07 years, participated in this study. The results of the intensive insulin regimen were evaluated after one-year follow-up in 11 patients, and in 8 patients at 18 months. In the first year of this study we aimed to bring about higher blood glucose than is generally advised, …


Molecular Genetics Of Breast Cancer, Emel Ergül, Ali̇ Sazci Jan 2001

Molecular Genetics Of Breast Cancer, Emel Ergül, Ali̇ Sazci

Turkish Journal of Medical Sciences

Recent advances in molecular biology enable researchers to understand the basis of breast cancer much better and show that hereditery breast cancer may result from mutations on several specific gene loci including BRCA1, BRCA2, p53, ATM and PTEN. These genes are tumor supressor genes and although their functions are different, they are all involved in the maintenance of genomic stability after DNA damage. Mutations that impair the function of these genes may adversely affect the manner in which DNA damage is processed. It is likely that the risk of breast cancer development is increased through this mechanism. Moreover, there are …


Teucrium Polium Inhibits Nerve Conduction And Carrageenan Induced Inflammation In The Rat Skin, Jumah Shakhanbeh, O. Adigüzel Jan 2001

Teucrium Polium Inhibits Nerve Conduction And Carrageenan Induced Inflammation In The Rat Skin, Jumah Shakhanbeh, O. Adigüzel

Turkish Journal of Medical Sciences

The effects of acute and chronic perineural treatment of the rat saphenous nerve with Teucrium polium (L.) extract on the conduction property of primary afferent nerve fibers and carrageenan-induced skin inflammation were studied. Direct application of 2.0% T. polium extract to the nerve trunk caused immediate complete inhibition of compound action potentials (CAPs) of all types of primary afferent nerve fibers. Partial recovery of CAPs was seen in up to 37.5% of Aab-fibers, 30.4% of Ad-fibers and 32.4% for C-fibers over one hour after removal of the extract. Fifteen to twenty days after a single perineural application of 2% T. …


The Antioxidant Activity Of The Leaves Of Cydonia Vulgaris, Ali̇ Yildirim, Müni̇r Oktay, Vahi̇t Bi̇laloğlu Jan 2001

The Antioxidant Activity Of The Leaves Of Cydonia Vulgaris, Ali̇ Yildirim, Müni̇r Oktay, Vahi̇t Bi̇laloğlu

Turkish Journal of Medical Sciences

The antioxidant activities of water, ethanol, and ether extracts of the leaves of Cydonia vulgaris Pers. were determined by the thiocyanate method. The antioxidant activity of water extract was increased with the increasing amount of extract (200 µg-1000µg) added to the linoleic acid emulsion. Ether extract was the most effective antioxidant among the extracts. Like antioxidant activity, the reducing power of water extract was concentration dependent. However, ethanol extract was the highest in reducing power, and ether extract was the lowest. The results obtained in the present study indicate that the leaves of Cydonia vulgaris are a potential source of …


A Relation Between The Apolipoprotein E Genotypes And Microalbuminuria In Type 2 Diabetes Mellitus, Ersi̇n Akarsu, İbrahi̇m Pi̇ri̇m, İlyas Çapoğlu, Hasan Kaya, Güngör Akçay, Necdet Ünüvar Jan 2001

A Relation Between The Apolipoprotein E Genotypes And Microalbuminuria In Type 2 Diabetes Mellitus, Ersi̇n Akarsu, İbrahi̇m Pi̇ri̇m, İlyas Çapoğlu, Hasan Kaya, Güngör Akçay, Necdet Ünüvar

Turkish Journal of Medical Sciences

Various relations between apolipoprotein E (apo E) and diabetic nephropathy have been reported. In this study, the question of whether there is any relation between apo E genotypes and microalbuminuria in patients with type-2 diabetes mellitus was examined. Twenty-one male and 25 female patients, with ages the range of 37-62 years (mean 51.7±7.5) with type-2 diabetes mellitus were studied. The apo E genotypes were determined by PCR amplification of the 227 bp region followed by CfoI digestion to release specific band patterns. While 24 patients had microalbuminuria (microalbuminuria group), 22 patients had normoalbuminuria (normoalbuminuria group). The patients with microalbuminuria had …


Cytomorphologic Analysis Of Papanicolaou Stained Smears Collected From Floor Of The Mouth Mucosa In Patients With Or Without Oral Malignancy, Nur Mollaoğlu, Jonathan George Cowpe, Rita Walker Jan 2001

Cytomorphologic Analysis Of Papanicolaou Stained Smears Collected From Floor Of The Mouth Mucosa In Patients With Or Without Oral Malignancy, Nur Mollaoğlu, Jonathan George Cowpe, Rita Walker

Turkish Journal of Medical Sciences

Cytology has been found to be a reliable and accurate technique when used to diagnose early malignant transformation in areas of the body where visual examination is difficult or impossible. The factors initiating neoplastic growth are still not clearly understood. Thus, automated instruments, capable of objective and quantitative cell analysis, have been used in descriptive morphology, for the assessment of tumour cell heterogeneity. Smears were collected from a group of 33 patients presenting with squamous cell carcinoma. Nine females and 24 males presenting with squamous cell carcinoma of the floor of the mouth underwent quantitative cytological assessment. These slides were …


Apolipoprotein-E Genotyping In Patients With Coronary Heart Disease, İbrahi̇m Pi̇ri̇m, Muhammet Fevzi̇ Polat, Ersi̇n Akarsu, Yaşar Nuri̇ Şahi̇n, Engi̇n Bozkurt Jan 2001

Apolipoprotein-E Genotyping In Patients With Coronary Heart Disease, İbrahi̇m Pi̇ri̇m, Muhammet Fevzi̇ Polat, Ersi̇n Akarsu, Yaşar Nuri̇ Şahi̇n, Engi̇n Bozkurt

Turkish Journal of Medical Sciences

The aim of the study was to establish apolipoprotein E genotype in the patients with coronary heart disease (CHD) who are inhabitants of the north-east of Turkey. The study involved 106 patients (70 male, 36 female; ages ranging from 37 to 69 years) who are local people and were angiocardiographically diagnosed for CHD. The risk factors such as smoking, alcohol and obesity were determined and serum triglyceride, total cholesterol, LDL and HDL were measured in the subjects. Each patient sample was apolipoprotein E genotyped, which was achieved by PCR amplification of the 227 bp region followed by Cfo I digestion …


Igg Subclass Levels In Elf In Copd, Demet Karnak, Sumru Beder, Oya Kayacan, Levent Karaca Jan 2001

Igg Subclass Levels In Elf In Copd, Demet Karnak, Sumru Beder, Oya Kayacan, Levent Karaca

Turkish Journal of Medical Sciences

Objective: Deficiency of immunoglobulin (Ig) may be responsible for recurrent infections in chronic obstructive pulmonary disease (COPD). Our aim was to investigate the level of IgG subclass (IgGsc) in the epithelial lining fluid (ELF) and to try to find a possible relation between these levels and serum or bronchoalveolar lavage (BAL) IgGsc levels in COPD patients with recurrent acute exacerbation. Methods: Twenty-four clinically stable non-smoking COPD patients who had developed recurrent exacerbations three or four times a year and 17 individuals without COPD were enrolled as the experimental and control groups (Group I and II) respectively. The BAL procedure was …


Determination Of The Absorption Of Zn By Preterm Infants, Using 70zn Enriched Tracer, Ulvi̇ Ulusoy, John E. Whitley Jan 2001

Determination Of The Absorption Of Zn By Preterm Infants, Using 70zn Enriched Tracer, Ulvi̇ Ulusoy, John E. Whitley

Turkish Journal of Medical Sciences

Absorption of zinc by preterm infants from a synthetic milk labelled extrinsically with 70Zn tracer was determined by faecal monitoring. Six male preterm babies with a mean gestational age of 31±2.9 weeks and a mean birth weight of 1.23±0.24 kg were studied for three balance periods of 72 h with intervals ranging from 6 to 30 days. The babies were fed with the milk containing zinc with added 70Zn tracer. The net (NA) and true (TA) absorption, and endogenous loss (EL) of zinc were determined by the classical (chemical) balance and isotopic approaches. The mean TA, NA and EL were …


A Comparative Clinical And Histopathological Examination Of Hemangioma And Chronic Inflammatory Enlargement Of Gingiva, Umur Sakallioğlu, Gökhan Açikgöz, Tuğrul Kirtiloğlu, Musa Aldikaçti Jan 2001

A Comparative Clinical And Histopathological Examination Of Hemangioma And Chronic Inflammatory Enlargement Of Gingiva, Umur Sakallioğlu, Gökhan Açikgöz, Tuğrul Kirtiloğlu, Musa Aldikaçti

Turkish Journal of Medical Sciences

Chronic inflammatory gingival enlargement (CIGE) occasionally exhibits some similarities with hemangioma (Hm) by means of the clinical and histopathological features of gingiva. When compared with hemangioma, CIGE is more frequently encountered by the dental profession. However, Hm is rarely seen on gingiva and it has a non-inflammatory nature and mostly congenital origin. The present paper reports the clinic and histopathologic features of CIGE and Hm of gingiva in patients referred to our clinic suffering primarily from gingival overgrowth. Twenty-one patients were diagnosed as having CIGE (in a 4-month period) and 10 patients were diagnosed as having Hm (in a 12-month …


A Father Born To Consanguineous Parents Had Double Translocation 21;22 [44,Xy, T(21q;22q), T(21q;22q)] Due To Familial Translocation In Four Generations, Cumhur Gündüz, Ferda Özkinay, Özgür Çoğulu, Gül Sapmaz, Tufan Çankaya, Ci̇hangi̇r Özkinay Jan 2001

A Father Born To Consanguineous Parents Had Double Translocation 21;22 [44,Xy, T(21q;22q), T(21q;22q)] Due To Familial Translocation In Four Generations, Cumhur Gündüz, Ferda Özkinay, Özgür Çoğulu, Gül Sapmaz, Tufan Çankaya, Ci̇hangi̇r Özkinay

Turkish Journal of Medical Sciences

No abstract provided.


Fine Structural Demonstration Of Anionic Sites On Streptococcal And Staphylococcal Envelopes By Cationic Dyes, Yosun Mater, Sabi̇re Karaçali Jan 2001

Fine Structural Demonstration Of Anionic Sites On Streptococcal And Staphylococcal Envelopes By Cationic Dyes, Yosun Mater, Sabi̇re Karaçali

Turkish Journal of Medical Sciences

Anionic sites on bacterial envelopes of Streptococcus \beta-haemolyticus and Staphylococcus aureus ATCC 6538 P were investigated on materials fixed with Karnovsky fixative containing the cationic dyes ruthenium red (RR) or alcian blue (AB). Electron dense granules and short fibrils were found to be irregularly distributed around the cells on the glycocalyces of both genera. An electron dense stained reaction with RR or AB on the cell membrane and glycocalyx indicates the presence of rich anionic sites on these structures. Electron microscopic observations demonstrate that anionic sites in the glycocalyx are responsible for the adherence phenomenon during the formation of microcolonies. …


Investigation Of Some Immune System Parameters And Gfap Immunoreactivity In Convulsive And Non-Convulsive Seizures In Rats, Ni̇lüfer Esen, Yeşi̇m Gürbüz, Deni̇z Şahi̇n, Nurbay Ateş Jan 2001

Investigation Of Some Immune System Parameters And Gfap Immunoreactivity In Convulsive And Non-Convulsive Seizures In Rats, Ni̇lüfer Esen, Yeşi̇m Gürbüz, Deni̇z Şahi̇n, Nurbay Ateş

Turkish Journal of Medical Sciences

The aim of this study was to investigate the systemic humoral and cell-mediated immune system parameters, and glial fibrillary acidic protein (GFAP) immunoreactivity in non-convulsive absence epilepsy and pentylenetetrazole (PTZ)- induced generalized tonic-clonic seizures. Animals were divided into three groups: i. the control group, ii. the genetic absence epilepsy WAG/Rij group, and iii. the chronic generalized tonic-clonic convulsion group injected with PTZ. After the experimental procedure, blood samples were collected intracardially, and CD3+ (T cells), CD4+ (T helper), CD8+ (T cytotoxic), CD19+ (B cells) and CD25+ (IL-2 reseptor, active T cell) cell ratios were determined by indirect immunoflourescence in FACScan, …


Optimization Of Fluorometric Measurement Of Free And Total Carnitine In Serum, Muhi̇tti̇n A. Serdar, İsmai̇l Kurt, İnci̇ Bayraktar, Levent Kenar, Fati̇h Özçeli̇k, İbrahi̇m Onur, Türker Kutluay Jan 2001

Optimization Of Fluorometric Measurement Of Free And Total Carnitine In Serum, Muhi̇tti̇n A. Serdar, İsmai̇l Kurt, İnci̇ Bayraktar, Levent Kenar, Fati̇h Özçeli̇k, İbrahi̇m Onur, Türker Kutluay

Turkish Journal of Medical Sciences

Carnitine is a major molecule which plays a great role in the transfer of long chain fatty acids into the mitochondrion. Measurement of its total and free forms is essential in the clinical evaluation of carnitine deficiency that can arise from disorders either primarily or secondarily. In this study, different deproteinization methods (precipitation with perchloric acid, heat denaturation of freeze thawing and filtration) were evaluated for the optimization of fluorometric measurement of total and free carnitine in serum. The most appropriate method for the carnitine measurement was established by additional studies of deacylation at different temperatures, pH optimization of the …


Isolated Caudate Nucleus Hemorrhage During The Acute Period Of Stroke, Selçuk Çomoğlu, Şenay Özbakir Jan 2001

Isolated Caudate Nucleus Hemorrhage During The Acute Period Of Stroke, Selçuk Çomoğlu, Şenay Özbakir

Turkish Journal of Medical Sciences

Caudate hemorrhage is a rare stroke syndrome that accounts for only 3-7% of all primary intracerebral hematomas. In this study, we evaluated eight patients with isolated caudate nucleus hemorrhage for their clinical presentation and neurological findings. Material and methods: Eight patients, who were admitted to our department with isolated caudate nucleus hemorrhage, were evaluated for their clinical presentation and present risk factor findings on neurological examination and prognosis. Results: The most common symptoms on admission were headache and vomiting (five patients), whereas the most common findings on neurological examination were unconsciousness and neck stiffness (four patients). Two patients who were …


Susceptibility Of Mycobacterium Tuberculosis Strains To First-Line And Second-Line Antituberculosis Drugs In Ege University Hospital, Candan Çi̇çek Saydam, Cengi̇z Çavuşoğlu, Di̇lek Burhanoğlu, Ni̇sel Özkalay, F. Zühre Badak, Altinay Bi̇lgi̇ç Jan 2001

Susceptibility Of Mycobacterium Tuberculosis Strains To First-Line And Second-Line Antituberculosis Drugs In Ege University Hospital, Candan Çi̇çek Saydam, Cengi̇z Çavuşoğlu, Di̇lek Burhanoğlu, Ni̇sel Özkalay, F. Zühre Badak, Altinay Bi̇lgi̇ç

Turkish Journal of Medical Sciences

Recently tuberculosis has shown a speedy worldwide spread. The incidence of drug-resistant Mycobacterium tuberculosis is increasing in almost all industrialized and developing countries. The epidemiology of multiple drug resistance varies in different regions and countries. The aim of this study was to determine the activities of first-line (isoniazid, rifampin, ethambutol and streptomycin) and second-line (kanamycin, para-aminosalicylic acid, ethionamide and capreomycin) antituberculosis drugs on 100 various clinical isolates of M. tuberculosis. Mycobacterium tuberculosis ATCC 27294, ATCC 35838, ATCC 35825 and ATCC 35837 were used for internal quality control. First-line drug resistant strains were isolated from 10 clinical specimens. Six of them …


Thalamic Hemorrhage (Presentation And Prognosis Of Hemorrhages), Şule Bi̇len, Selçuk Çomoğlu, Yaşar Şahi̇n, Şenay Özbakir Jan 2001

Thalamic Hemorrhage (Presentation And Prognosis Of Hemorrhages), Şule Bi̇len, Selçuk Çomoğlu, Yaşar Şahi̇n, Şenay Özbakir

Turkish Journal of Medical Sciences

The thalamus is one of the most vulnerable areas for intracerebral hemorrhages. In this study, we investigated the parameters of thalamic hemorrhage such as risk factors, initial complaints, neurologic findings and the prognostic effects of the presence or absence of ventricular penetration, as well as the volume and size of hematoma. A total of 57 patients with thalamic hemorrhage, whose diagnosis was confirmed by cranial computerized tomography, were evaluated. The most significant risk factor was hypertension, while the most common initial clinic symptom was incontinence. Those patients who had small hemorrhagic size of low volume and the presence of ventricular …


Serum Triglyceride And Lipoprotein-Cholesterol Levels In Patients With And Without Luteal Phase Defect, Hi̇kmet Hassa, Ömer T. Yalçin, Atti̇la Yildirim, H. Mete Tanir, Özgül Paşaoğlu, Mi̇ne İnan Jan 2001

Serum Triglyceride And Lipoprotein-Cholesterol Levels In Patients With And Without Luteal Phase Defect, Hi̇kmet Hassa, Ömer T. Yalçin, Atti̇la Yildirim, H. Mete Tanir, Özgül Paşaoğlu, Mi̇ne İnan

Turkish Journal of Medical Sciences

Objective: To evaluate the serum triglyceride and lipoprotein-cholesterol levels in subjects with and without luteal phase defect. Method: Early follicular and midluteal phase serum triglycerides, lipoprotein-cholesterol and progesterone levels were assessed in 19 subjects with and in 73 subjects without luteal phase defect. The criterion for diagnosis of luteal phase defect was histological development of endometrium delayed for more than 2 days according to the chronological day. The data were analyzed by paired and unpaired t tests, Chi-square test and correlation analysis. Results: The early follicular serum triglyceride and lipoprotein-cholesterol levels of the two groups were similar (p>0.05). The …


The Large Aortopulmonary Window Without Pulmonary Vascular Disease, Yahya Ünlü, Naci̇ Cevi̇z, Bi̇lgehan Erkut, Yusuf Veli̇oğlu, Hi̇kmet Koçak Jan 2001

The Large Aortopulmonary Window Without Pulmonary Vascular Disease, Yahya Ünlü, Naci̇ Cevi̇z, Bi̇lgehan Erkut, Yusuf Veli̇oğlu, Hi̇kmet Koçak

Turkish Journal of Medical Sciences

LÜ^1 Naci CEVİZ^2 Bilgehan ERKUT^1 Yusuf VELİOĞLU^1 Hikmet KOÇAK^1 Departments of ^1Cardiovascular Surgery, ^2Pediatric Cardiology, Faculty of Medicine, Atatürk University, Erzurum - TURKEY


Ocular Toxocariasis With Bilateral Virtually Symmetrical Optic Nerve Head Granulomas, Gülay Güllülü, İlknur Akyol Salman, Ahmet Özbek, Nesli̇han Astam, Akin Levent Jan 2001

Ocular Toxocariasis With Bilateral Virtually Symmetrical Optic Nerve Head Granulomas, Gülay Güllülü, İlknur Akyol Salman, Ahmet Özbek, Nesli̇han Astam, Akin Levent

Turkish Journal of Medical Sciences

LLÜLÜ^1 İlknur AKYOL^1 Ahmet ÖZBEK^2 Neslihan ASTAM^1 Akın LEVENT^3 Departments of ^1Ophthalmology, ^2Microbiology and Clinical Microbiology, ^3Radiology, Faculty of Medicine, Atatürk University, Erzurum - TURKEY


A Case Of Bisatellited-Isodicentric Supernumerary Chromosome 15, Y. Selma Süngü, Bi̇rsen Karaman, E. Ferda Perçi̇n, Sevi̇m Balci, İlhan Sezgi̇n Jan 2001

A Case Of Bisatellited-Isodicentric Supernumerary Chromosome 15, Y. Selma Süngü, Bi̇rsen Karaman, E. Ferda Perçi̇n, Sevi̇m Balci, İlhan Sezgi̇n

Turkish Journal of Medical Sciences

SÜNGÜ^1 Birsen KARAMAN^2 E. Ferda PERÇİN^1 Sevim BALCI^3 İlhan SEZGİN^1 ^1Department of Medical Biology and Genetics, Faculty of Medicine, Cumhuriyet University 58140-Sivas, ^2Department of Medical Genetics, Faculty of Medicine, Istanbul University, Çapa -Istanbul, ^3Department of Pediatrics, Faculty of Medicine, Hacettepe University Ankara - TURKEY


Antifungal Activities Of Different Extracts And Echimidine-N-Oxide From Symphytum Sylvaticum Boiss. Subsp. Sepulcrale (Boiss. & Bal.) Greuter & Burdet Var. Sepulcrale, Murat Kartal, Semra Kurucu, Muhammad I. Choudary Jan 2001

Antifungal Activities Of Different Extracts And Echimidine-N-Oxide From Symphytum Sylvaticum Boiss. Subsp. Sepulcrale (Boiss. & Bal.) Greuter & Burdet Var. Sepulcrale, Murat Kartal, Semra Kurucu, Muhammad I. Choudary

Turkish Journal of Medical Sciences

Antifungal activity was determined by the tube dilution method on different extracts of Symphytum sylvaticum Boiss. subsp. sepulcrale (Boiss. & Bal.) Greuter & Burdet var. sepulcrale. The antifungal activity of the isolated compound from the root alkaloid fraction (Echimidine-N-Oxide) was also tested against ten fungal cultures. The activity was found to be mainly due to Echimidine-N-Oxide (ENO). The quantitative determination of ENO was also carried out with capillary gas chromatography in the roots and the aerial parts of the plant.


Comparison Of Remifentanil-Propofol And Sevoflurane For Preventing Cardiovascular Response And Quality Of Recovery In Paediatric Otolaryngologic Surgery, Zeli̇ha Özer Koçak, Ali̇ Aydin Altunkan, Şebnem Atici, İsmai̇l Ci̇nel, Uğur Oral Jan 2001

Comparison Of Remifentanil-Propofol And Sevoflurane For Preventing Cardiovascular Response And Quality Of Recovery In Paediatric Otolaryngologic Surgery, Zeli̇ha Özer Koçak, Ali̇ Aydin Altunkan, Şebnem Atici, İsmai̇l Ci̇nel, Uğur Oral

Turkish Journal of Medical Sciences

As the practice of outpatient paediatric surgery advances, the search continues for anaesthesia that provides rapid smooth induction, stable patient haemodynamics, rapid emergence and minimal unpleasant side effects. Therefore, this study was designed to compare the haemodynamic changes, emergence and recovery characteristics of remifentanil-propofol (TIVA) and sevoflurane anaesthesia for adenoidectomy with bilateral myringotomy and insertion of tubes and/or tonsillectomy (ENT surgery) in children. Children aged 6.3±1.6 years, undergoing elective ENT surgery, were randomly assigned to receive TIVA (n=15) and sevoflurane (n=15). In both groups, anaesthesia was induced with propofol and remifentanil and maintained either with infusion of propofol-remifentanil or sevoflurane …


Production Of Monoclonal Antibody Specific For Human Hemoglobin, Selma Öztürk, Beyazit Çirakoğlu Jan 2001

Production Of Monoclonal Antibody Specific For Human Hemoglobin, Selma Öztürk, Beyazit Çirakoğlu

Turkish Journal of Medical Sciences

Hemoglobin is not normally present in sputum, urine or feces except in minute amounts. Demonstration of the presence of hemoglobin in these materials is an important diagnostic clue that usually implies bleeding within the respiratory, urinary or gastroentestinal tract, respectively. In this work, a hybridoma line secreting monoclonal antibody (Mab) highly specific for human hemoglobin A (hHbA) was generated by fusion of immunized BALB/c mouse splenic lymphocytes with non-secreting mouse myeloma cells followed by selection in hypoxanthine-aminopterin-thymidine (HAT) medium and the screening of culture fluids for hHb A affinity in ELISA. The Mab produced by MAM 2/3B6 is highly specific …


Flow Cytometric Analysis Of The Effects Of Methotrexate And Vepesid On The Hep-2 Cell Cycle, Ni̇zami̇ Duran, Adi̇l M. Allahverdi̇yev, Sali̇h Çeti̇ner Jan 2001

Flow Cytometric Analysis Of The Effects Of Methotrexate And Vepesid On The Hep-2 Cell Cycle, Ni̇zami̇ Duran, Adi̇l M. Allahverdi̇yev, Sali̇h Çeti̇ner

Turkish Journal of Medical Sciences

Objective: To determine the mechanism of action of Methotrexate and Vepesid on the HEp-2 cells isolated from human laryngeal cancer cells morphologically and flow cytometrically (G1, G2, S and PI). Materials and Methods: The HEp-2 continuous cell line was used. Cultivation of the cells was realized in EMEM medium with 10% fetal bovine serum at an atmosphere of 37ºC with 5% CO_2. Six different concentrations of Vepesid and Methotrexate were prepared by diluting with deionized water (5 µg/ml, 50 mg/ml, 500 µg/ml). The morphological and cell cycle parameters of HEp-2 cells were determined by inverted microscope and flow cytometer respectively. …


The Effects Of Benzo(A) Pyrene Doxorubicin And Paclitaxel On P170 Glycoprotein, Di̇dem Turgut Coşan, Ayşe Başaran Jan 2001

The Effects Of Benzo(A) Pyrene Doxorubicin And Paclitaxel On P170 Glycoprotein, Di̇dem Turgut Coşan, Ayşe Başaran

Turkish Journal of Medical Sciences

B(a)P is a mutagenic, carcinogenic and teratogenic substance. Paclitaxel and doxorubicin are antineoplastic drugs widely used in cancer treatment. The purpose of this study is to observe the effects of doxorubicin and paclitaxel on p170 glycoprotein in rat liver and kidney tissue after administration of B(a)P. As is well known, p170 glycoprotein is an indicator of drug resistance. We hypothesized that a combination of these antineoplastic drugs would cause lower p170 levels and thus would have a stronger effect than single drug administration. For confirmation, a combination of drugs was used to prevent the development of drug resistance. In this …


Protective Effects Of Vitamin E Selenium And Allopurinol Against Stress-Induced Ulcer Formation In Rats, Nai̇me Canoruç, Ramazan Çi̇çek, Aytaç Atamer, Mehmet Dursun, Cengi̇z Turgut, Ensari̇ Güneli̇, Fi̇kri̇ Canoruç Jan 2001

Protective Effects Of Vitamin E Selenium And Allopurinol Against Stress-Induced Ulcer Formation In Rats, Nai̇me Canoruç, Ramazan Çi̇çek, Aytaç Atamer, Mehmet Dursun, Cengi̇z Turgut, Ensari̇ Güneli̇, Fi̇kri̇ Canoruç

Turkish Journal of Medical Sciences

This study was carried out in the Health Research Center (DÜSAM) laboratory of Dicle University. Vitamin E, Selenium and Allopurinol were given to counteract gastric damage caused by stress in rats. In the study 64 male Sprague dawley rats were used. They were divided into 8 groups, and protective agents were applied at different doses for 8 weeks. Then, performing cold-restraint stress stomach tissues were taken out and investigated macroscopically to determine mucosal damage. Lipid peroxidation levels in the stomach specimens were determined by identifying Malondialdehyde (MDA) levels by the thiobarbituric acid method. These agents have shown protective effects against …


In Vitro Mic Determination Of Haemophilus Influenzae Strains Using E-Test, Ni̇lay Çöplü, Orhan Cem Aktepe, Selda Uluutku, Engi̇n Güvener Jan 2001

In Vitro Mic Determination Of Haemophilus Influenzae Strains Using E-Test, Ni̇lay Çöplü, Orhan Cem Aktepe, Selda Uluutku, Engi̇n Güvener

Turkish Journal of Medical Sciences

H. influenzae is a fastidious bacteria, so the selection of a reliable method for in-vitro susceptibility tests is particularly important. The E-test is a practical method for detecting in-vitro antimicrobial susceptibility of antibiotics by MIC values. This study was performed using the E-test for direct patient management and in order to obtain epidemiological data on H. influenzae. The detection of antimicrobial agent resistance among fifty-seven H. influenzae isolates from lower respiratory tract infections in childhood was studied by the E-test. The E-test was performed on HTM agar according to the instruction manual recommendations. The susceptibility ratios and the MIC90 values …


Effects Of Experimental Diabetes And Insulin Treatment On Rabbit Renal Morphology: A Quantitative And Qualitative Study, Hüseyi̇n Eray Bulut, Bi̇lge Onarlioğlu, Celal Kaloğlu, Öztürk Özdemi̇r, Semi̇h Ayan Jan 2001

Effects Of Experimental Diabetes And Insulin Treatment On Rabbit Renal Morphology: A Quantitative And Qualitative Study, Hüseyi̇n Eray Bulut, Bi̇lge Onarlioğlu, Celal Kaloğlu, Öztürk Özdemi̇r, Semi̇h Ayan

Turkish Journal of Medical Sciences

Diabetes causes metabolic defects and related vascular alterations such as atherosclerosis. These vascular alterations may also reflect the renal structural degeneration under the influence of diabetes. Therefore, the aim of the present study was to determine qualitatively and quantitatively the effect of experimental diabetes on rabbit renal cortical structure, and also to evaluate the reversal rate of the renal structural alterations by daily insulin treatment. Eighteen rabbits, bred and fed in standard laboratory conditions, were divided into 3 groups each containing 6 animals. While control group animals received no treatment, the rest were injected with a single dose of intravenous …


Diabetic Osteopathy: Who Is At Risk?, Serap Demi̇r, Ayşi̇n Öge, Selma Karaahmetoğlu, Osman Müftüoğlu, Seni̇ha Naldöken Jan 2001

Diabetic Osteopathy: Who Is At Risk?, Serap Demi̇r, Ayşi̇n Öge, Selma Karaahmetoğlu, Osman Müftüoğlu, Seni̇ha Naldöken

Turkish Journal of Medical Sciences

This study was designed to determine the prevalence of osteopathy in diabetic patients, who are at risk of developing osteopathy, and clarify the relationship between osteopathy and other complications of diabetes mellitus. Thirty-four type I and 66 type II diabetic patients admitted to the endocrinology clinic between 1996 and 1997 and 30 nondiabetic subjects as a control group were studied. Bone scintigraphy (Tc 99m methylene diphosphonate) was used for the diagnosis of osteopathy. The body mass index (BMI) of each patient was calculated. Polyneuropathy was assessed by electromyography and the patients, for each of whom BMI was calculated, were asked …