Medicine and Health Sciences Commons^™

Duck Plague Virus An Investigation Of Cytopathic Effects, In-Vitro Host Range, And Inhibition By Phosphonoacetic Acid, Ronald Attanasio

Biological Sciences Theses & Dissertations

Duck plague is a hemorrhagic, necrotic disease found exclusively in the order Anseriformes, family Anatidae (ducks, geese, swans). The etiologic agent has been reported to be a herpes-like virus. The Holland strain of duck plague was found to induce cytopathic effects in cell culture (perinuclear vacuolation followed by lysis). A simple reproducible plaque assay for quantitating the virus was developed. Under conditions of the plaque assay, one-hit kinetics were observed. The host range of duck plague virus in cell culture was found to include the order Galliformes, family Phasianidae (pheasants, quails and partridges). Duck plague virus-induced cell culture cytopathic effects …

The Pathophysiology Of Acute Venous Occlusion, Lewis H. Curd Jr.

Biological Sciences Theses & Dissertations

Large artery, pedal small vein, femoral vein and central venous pressures and extremity volume changes were recorded in mongrel dogs in which the hindlimb was perfused at a constant flow. Arterial, pedal venous and femoral venous blood gases were drawn and analyzed for measurements of arterio-venous O₂ and CO₂ differences. Paw and thigh resistances were calculated. Measurements were repeated following ligation of the iliac and femoral veins, alpha receptor blockade (dibenzyline), and dilation with Vasodilan. Results indicate that ligation of extremity veins results in an active ·increase in total limb resistance below the ligation, an increase in the …

Effectiveness Of The Phase Contrast Microscope In Dental Patient Motivation, Denise Marie Bowen

Biological Sciences Theses & Dissertations

The purpose of this investigation was to examine the effectiveness of the phase contrast microscope when used as an adjunct to a patient education program in motivating individuals to improve oral hygiene habits. A convenience sample with randomized groups was utilized to select sixty-five subjects. Subjects attended five appointments: one appointment for oral prophylaxis, three appointments for oral hygiene instruction and a three month recall. A phase contrast microscope demonstration was the independent variable. Plaque scores were measured at each appointment using a Modification of the OHI-S. No statistically significant difference existed between the groups at any coinciding appointments. Statistically …

The Acquisition Of Dental Skills: Value Of Exercise In Teaching Instrumentation, Deanne Shuman Allen

Biological Sciences Theses & Dissertations

The purpose of this investigation was to determine if there were significant differences in correct dental skill performance through manipulation of the independent variable, hand exercises, and the assigned independent variable, students' hobbies.

A Three-Group, nonrandomized, posttest only design utilizing a sample of 48 first-year dental hygiene students was developed. Subjects were assigned to groups on the basis of hobby questionnaire scores. All subjects received the traditional pre-clinical course of instruction. The study entailed a four-week period in which subjects were instructed in explorer instrumentation techniques followed by the administration of a Performance Examination.

A Pearson r between hobby questionnaire …

Kinetic And Binding Studies On L-∝-Glycerophosphate Dehydrogenase, Paul Richard Rosevear

Chemistry & Biochemistry Theses & Dissertations

The properties of the coenzyme, NAD, binding site of chicken muscle L-∝-glycerophosphate dehydrogenase were studied. Adenosine monophosphate, adenosine diphosphate and adenosine diphosphoribose were sha.vn to inhibit the enzyme competitively with respect to NAD. The presence of adenosine, pyrophosphate and ribose regions in the coenzyme binding site of the enzyme was suggested by the inhibitor constants obtained for these compounds.

Disodium monoalkyl phosphates, n-butyl to n-dodecyl phosphate, inclusive, were also shown to inhibit the L-∝-glycerophosphate dehydrogenase catalized reaction competitively with respect to NAD. A positive chain length effect was observed in the binding of these compounds to the enzyme, suggesting the …

A Batch Method For The Isolation Of Immunogenically Pure Igg, Iga, And Igm From Human Sera, Samuel Christopher Powell

Chemistry & Biochemistry Theses & Dissertations

A method is described for preparing an enriched fraction of the three major antibodies, immunoglobulins IgG, IgA and IgM, from normal human serum by a batch method. Selective precipitation of these proteins was carried out in successive steps using polyethylene glycol (PEG) of average molecular weight 6,000 at concentrations of 7%, 12% and 14% (16) . Zinc sulfate (10 mM) was used to remove highly immunogenic alpha₂ macroglobulin from the IgM fraction. IgA, IgM and IgG were further purified by adsorption methods using DEAE-cellulose. Quantitation and qualitation of the enriched fractions showed nearly complete separation of each of the …

Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoretic Studies Demonstrating Cytoplasmic Protein Components Unique To The Mycelial Phase And To The Yeast Phase Of Candida Albicans, Theresa Catherine Burgess

Biological Sciences Theses & Dissertations

Blastospore and mycelial cytoplasmic extracts from four strains of Candida albicans, varying in virulence, were compared by sodium dodecyl sulfate (SDS) polyacrylamide disc gel electrophoresis Cytoplasmic extracts were made by disrupting the cultures in a Braun homogenizer followed by ultracentrifugation. The supernatant fractions were then resolved on polyacrylamide gels containing SDS. Each of the preparations yielded a distinct pattern. There were mycelial specific and blastospore specific protein bands. There were more components resolved from the blastospore phase cytoplasmic extracts than from the mycelial phase cytoplasmic extracts. There were bands common to both phases. These results demonstrate protein components unique …

Seasonal Distribution Of Higher Marine And Aquatic Fungi In The Back Bay National Wildlife Refuge, Scott Schatz

Biological Sciences Theses & Dissertations

Abstract unavailable.

The Effect Of Training On The Attitudes Of Freshmen Professional Nursing Students Toward Death And Dying, Yolanda Cardelli Hampel

Teaching & Learning Theses & Dissertations

No abstract.

The Effect Of Various Doses Of Caffeine Upon The Maximal Muscular Endurance Of College Females, Roberta Jane Perkins

Human Movement Studies & Special Education Theses & Dissertations

A limited number of investigations dealing with the effects of caffeine upon human physical performance have been conducted. Since caffeine is a central nervous system stimulant and it is a constituent of the popularly-consumed beverages - coffee, tea, and cola drinks, it would appear that numerous studies would have been conducted in order to determine the effect of caffeine upon human muscular performance. At any rate, there is a dearth of studies on this topic and the findings of the conducted experiments are equivocal and ambiguous.

The purpose of the present study was to determine the effects of various doses …

The Effect Of Pre-Exposure To Sucrose On The Acquisition And Extinction Of A Conditioned Aversion, Shirlee Fenwick

Psychology Theses & Dissertations

Twenty-eight female, water deprived rats were given 5 minutes access to sucrose for either 1,7,14, or 21 days. They were then given LiC1 injections every third day until an aversion to the sucrose developed. The results indicated that as amount of pre-exposure to sucrose increased, the number of illnesses required to develop an aversion increased. Also, the rate of extinction of the conditioned aversion was inversely related to the number of exposures required. to establish the aversion.

Comparison Of Interferon Produced In Vitro By Monkey Kidney Cells Demonstrating Interference Against Polio Ii, Coxsackie A-9, Influenza A2/Hong Kong/68, And Echo-9 Viruses, Archer Duncan Farmer Jr.

Biological Sciences Theses & Dissertations

Abstract unavailable

Phytoplankton Succession In The Lafayette River, Estuary, Norfolk, Virginia, Thomas W. Purcell Lll

Biological Sciences Theses & Dissertations

Abstract unavailable.

Advanced Placement Guidelines For Schools Of Practical Nursing For Placement Of Nursing Assistants, Leona Casner Wyatt

Educational Leadership & Workforce Development Theses & Dissertations

No abstract.

A Study Of Precipitin And Soluble Antigen Fluorescent Antibody Technique In The Detection Of Dirofilaria Immitis In Canines, John H. Ellsworth

Biological Sciences Theses & Dissertations

Abstract unavailable.

A Follow-Up Survey Of The Graduates Of The Cooperative Program Of The Norfolk City Schools And The Leigh Memorial Hospital School Of Licensed Practical Nursing, Joseph Patrick Clarke

Educational Leadership & Workforce Development Theses & Dissertations

No abstract provided.

Narrow Band Modification For Speech Testing Of The Cochlear Reserve By Threshold Shift, Michael F. Seidemann

Communication Disorders & Special Education Theses & Dissertations

No abstract.

The Relationship Of Three Selected Admission Variables To Student Achievement In A Diploma School Of Nursing, Elsie M. Futrell

Educational Leadership & Workforce Development Theses & Dissertations

No abstract.

Some Selected Characteristics Of A Dental Assistant Education Program Based On Certain Needs Of The Commonwealth Of Virginia, Gene W. Hirschfeld

Educational Leadership & Workforce Development Theses & Dissertations

No abstract.

A Survey Of Community Services Used By Students Enrolled In The Kirk-Cone Rehabilitation Center, 1965-1966, Audrey Hackett Crandall

Educational Leadership & Workforce Development Theses & Dissertations

No abstract.