Medicine and Health Sciences Commons^™

Electroporation-Mediated Gene Transfer Directly To The Swine Heart, Barbara Hargrave, Harre Downey, Cathryn Lundberg, Annelise Israel, Yeong-Jer Chen, Richard Heller

Bioelectrics Publications

In vivo gene transfer to the ischemic heart via electroporation holds promise as a potential therapeutic approach for the treatment of heart disease. In the current study, we investigated the use of in vivo electroporation for gene transfer using three different penetrating electrodes and one non-penetrating electrode. The hearts of adult male swine were exposed through a sternotomy. Eight electric pulses synchronized to the rising phase of the R wave of the electrocardiogram were administered at varying pulse widths and field strengths following an injection of either a plasmid encoding luciferase or one encoding green fluorescent protein. Four sites on …

The Effects Of A Semi-Rigid Ankle Brace On A Simulated Isolated Subtalar Joint Instability, Julie Choisne, Matthew C. Hoch, Sebastian Bawab, Ian Alexander, Stacie I. Ringleb

Mechanical & Aerospace Engineering Faculty Publications

Subtalar joint instability is hypothesized to occur after injuries to the calcaneofibular ligament (CFL) in isolation or in combination with the cervical and the talocalcaneal interosseous ligaments. A common treatment for hindfoot instability is the application of an ankle brace. However, the ability of an ankle brace to promote subtalar joint stability is not well established. We assessed the kinematics of the subtalar joint, ankle, and hindfoot in the presence of isolated subtalar instability, investigated the effect of bracing in a CFL deficient foot and with a total rupture of the intrinsic ligaments, and evaluated how maximum inversion range of …

Disparate Health Implications Stemming From The Propensity Of Elderly And Medically Fragile Populations To Shelter In Place During Severe Storm Events, Joshua G. Behr, Rafael Diaz

VMASC Publications

Chronic conditions, disability limitations (mobility, cognitive, and sensory), and the need for assistance with activities of daily living are characteristics of elderly and medically fragile populations. Theory suggests that households with these vulnerability attributes are more likely to suffer storm-induced adverse and prolonged health consequences and, therefore, ought to evidence an increased propensity to evacuate prior to a severe storm event. Yet despite being more sensitive to storm disruption, the elderly and medically fragile populations are only slightly more likely to evacuate in the face of impending storms. This suggests, for these groups, there may be other factors such as …

Evaluation Of A Novel Rapid Diagnostic Test For Schistosoma Haematobium Based On The Detection Of Human Immunoglobulins Bound To Filtered Schistosoma Haematobium Eggs, Johnathan M. Sheele, Jimmy H. Kihara, Sarah Baddorf, Jonathan Byrne, Bhaskara Ravi

Mathematics & Statistics Faculty Publications

Objectives To determine whether the detection of human IgG bound to Schistosoma haematobium eggs from filtered urine could be used as a rapid diagnostic test (RDT-Sh).

Methods We filtered 160 urine samples from children in the Kwale District of Kenya to isolate S.haematobium eggs and used anti-human IgG antibody conjugated to horseradish peroxidase to bind to the human IgG attached to the eggs. We then added 3,35,5-tetramethylbenzidine base (TMB), which turns blue in the presence of horseradish peroxidase to detect the S.haematobium eggs. The RDT-Sh was compared in a blinded manner to urine microscopy.

Results The RDT-Sh was positive in …

Unplanned Drinking And Alcohol-Related Problems: A Preliminary Test Of The Model Of Unplanned Drinking Behavior, Matthew R. Pearson, James M. Henson

Psychology Faculty Publications

Much research links impulsivity with alcohol use and problems. In 2 studies, unplanned (or impulsive) drinking is assessed directly to determine whether it has direct effects on alcohol use and alcohol-related problems. In Study 1, we examined whether unplanned drinking serves as a proximal mediator of the effects of impulsivity-like traits on alcohol-related outcomes. With a sample of 211 college student drinkers, we found that the Unplanned Drinking Scale was significantly related to alcohol use, and perhaps more important, had a direct effect on alcohol-related problems even after controlling for frequency and quantity of alcohol use. Furthermore, unplanned drinking partially …

Alcohol Use And Trauma Exposure Among Male And Female Veterans Before, During, And After Military Service, Michelle L. Kelley, Jennifer Runnals, Matthew R. Pearson, Marinell Miller, John A. Fairbank, Va Mid-Atlantic Mirecc Women Veterans Workgroup

Psychology Faculty Publications

Background: The present study examined lifespan and combat-related trauma exposure as predictors of alcohol use among male and female veterans. Posttraumatic stress and depressive symptoms were examined as mediators of the effects of trauma exposure on alcohol use.

Methods: Data were examined from 1825 (1450 male, 375 female) veterans and active duty service members who took part in a multi-site research study conducted through the Department of Veterans Affairs Mid-Atlantic Mental Illness Research, Education and Clinical Center (VISN 6 MIRECC).

Results: For both men and women, depressive symptoms significantly mediated the effects of non-combat trauma exposure experienced before, during and …

Perceptions Of Approved Clinical Instructors: Barriers In The Implementation Of Evidence-Based Practice, Dorice A. Hankemeier, Bonnie L. Van Lunen

Rehabilitation Sciences Faculty Publications

Context: As evidence-based practice (EBP) becomes prevalent in athletic training education, the barriers that Approved Clinical Instructors (ACIs) experience in implementing it with students need to be understood.

Objective: To investigate barriers ACIs face when implementing EBP concepts in clinical practice and in teaching EBP to professional athletic training students and to investigate the educational emphases to improve the barriers.

Design: Qualitative study.

Setting: Telephone interviews.

Patients or Other Participants: Sixteen ACIs (11 men, 5 women; experience as an athletic trainer = 10 ± 4.7 years, experience as an ACI = 6.81 ± 3.9 years) were interviewed.

Data Collection and …

A Brief Motivational Intervention For Heavy Alcohol Use In Dental Practice Settings: Rationale And Development, James A. Neff, Scott T. Walters, Abby L. Braitman, Michelle L. Kelley, Michele L. Darby, Margaret F. Lemaster

Psychology Faculty Publications

Although brief alcohol interventions have proven effective in a variety of health care settings, the present article describes the development of the first brief intervention for heavy drinkers in dental practice. Elements of motivational interviewing and personalized normative feedback were incorporated in a 3- to 5-minute intervention delivered by dental hygienists. The intervention is guided by a one-page feedback report providing personalized normative feedback regarding the patient's current oral health practices, their drinking in comparison to others, and oral cancer risk associated with current smoking and drinking. Future publications will present data regarding intervention effectiveness from an ongoing randomized trial.

Nanosecond Pulsed Electric Field (Nspef) Ablation As An Alternative Or Adjunct To Surgery For Treatment Of Cancer, Ru Chen, Xinhua Chen, Stephen J. Beebe

Bioelectrics Publications

Surgery as resection or transplantation remains a fundamental means for cancer treatment and often offers an opportunity for a cure. However, surgery is not always possible because of tumor proximity to blood vessels or ducts or when a patient is not healthy enough to undergo surgery. Application of nanosecond pulsed electric fields (nsPEFs) is a new approach to treat cancer using pulse power technology that was originally designed for military purposes. This novel approach deposits extremely short pulses of high power, low energy electric fields into malignant tissues using electrodes to encompass tumors. Pre-clinical studies show that treatments are effective …

The Relation Of Socioeconomic Status, Parental Education, Vocabulary And Language Skills Of Children Who Stutter, Corrin G. Richels, Kia N. Johnson, Tedra A. Walden, Edward G. Conture

Communication Disorders & Special Education Faculty Publications

Purpose: The purpose of this project was to investigate the possible relation between standardized measures of vocabulary/language, mother and father education, and a composite measure of socioeconomic status (SES) for children who do not stutter (CWNS) and children who stutter (CWS).

Methods: Participants were 138 CWNS and 159 CWS between the ages of 2;6 and 6;3 and their families. The Hollingshead Four Factor Index of Social Position (i.e., Family SES) was used to calculate SES based on a composite score consisting of weighted values for paternal and maternal education and occupation. Statistical regression analyses were conducted to investigate the relation …

Special Issue On Medical Simulation, Michel Audette, Hanif M. Ladak

Computational Modeling & Simulation Engineering Faculty Publications

We would like to welcome you to this Special Issue on Medical Simulation, the first of its kind not only for SIMULATION: Transactions of The Society for Modeling and Simulation International, but for any technical journal. Our respective backgrounds are an indication of the technical and clinical breadth of medical simulation, as we approach the subject as primarily medical image analysis and biomechanics experts respectively, each with a variety of clinical interests spanning virtual reality (VR)–based neuro-, orthopedic and ear-nose-and-throat surgery. Moreover, we believe that the breadth of the papers that comprise this issue reflects an even broader perspective. After …

Use Of Evidence-Based Practice Among Athletic Training Educators, Clinicians, And Students, Part 2: Attitudes, Beliefs, Accessibility, And Barriers, Cailee W. Mccarthy, Dorice A. Hankemeier, Jessica M. Walter, Eric J. Newton, Bonnie L. Van Lunen

Rehabilitation Sciences Faculty Publications

Context: Successful implementation of evidence-based practice (EBP) within athletic training is contingent upon understanding the attitudes and beliefs and perceived barriers toward EBP as well as the accessibility to EBP resources of athletic training educators, clinicians, and students.

Objective: To assess the attitudes, beliefs, and perceived barriers toward EBP and accessibility to EBP resources among athletic training educators, clinicians, and students.

Design: Cross-sectional study.

Setting: Online survey instrument.

Patients or Other Participants: A total of 1209 athletic trainers participated: professional athletic training education program directors (n = 132), clinical preceptors (n = 266), clinicians (n = 716), postprofessional athletic training …

Use Of Evidence-Based Practice Among Athletic Training Educators, Clinicians, And Students, Part 1: Perceived Importance, Knowledge, And Confidence, Dorice A. Hankemeier, Jessica M. Walter, Cailee W. Mccarty, Eric J. Newton, Stacy E. Walker, Shana L. Pribesh, Beth E. Jamali, Sarah A. Manspeaker, Bonnie L. Van Lunen

Rehabilitation Sciences Faculty Publications

Context:

Although evidence-based practice (EBP) has become more prevalent, athletic trainers' perceptions of importance and knowledge of these concepts and their confidence in EBP are largely unknown.

Objective:

To assess perceived importance and knowledge of and confidence in EBP concepts in athletic trainers in various roles and with different degree levels.

Design:

Cross-sectional study.

Setting:

Online survey instrument.

Patients or Other Participants:

The survey was sent to 6702 athletic training educators, clinicians, and postprofessional students. A total of 1209 completed the survey, for a response rate of 18.04%.

Main Outcome Measure(s):

Demographic information and perceived importance and knowledge of and …

Erosive Hand Osteoarthritis Is Associated With Subclinical Atherosclerosis And Endothelial Dysfunction, Athanasios Koutroumpas, Athanasios Giannoukas, Elias Zintzaras, Ekaterini Exarchou, Aris Baliakos, Konstantinos Makaritsis, Lazaros I. Sakkas

Biological Sciences Faculty Publications

Chronic inflammatory disorders have been associated with accelerated atherosclerosis and increased cardiovascular (CV) risk. Recent evidence suggests that erosive hand osteoarthritis (EOA) has considerable inflammation; therefore, we examined the presence of subclinical atherosclerosis and endothelial dysfunction in EOA. Twenty-four patients with EOA and 24 age- and sex-matched healthy individuals without clinical OA were included in the study. No subject had a history of CV disease. Intima-media thickness (IMT) and atheromatous plaques in the common carotid and common femoral arteries were measured by Doppler ultrasonography. The endothelium-dependent, flow-mediated dilatation (FMD) and endothelium-independent, sublingual glyceryl trinitrate (NTG)-induced dilatation (NMD) of the brachial …

Assistive Technology: Fixing Humans, Petros J. Katsioloudis, Millie Jones

STEMPS Faculty Publications

The need for assistive technology--technology that assists persons with disabilities--is great. At no time has there been a greater need to provide assistive technologies than to assist the thousands of military veterans returning home from the Middle East with a range of physical and mental disabilities. Mobility impairment is a significant concern for many returning veterans. Assistive technologies are playing a major role in assisting war veterans returning with physical limitations through the loss of a limb or, in many cases, several limbs. This article presents the history and background of assistive technology and how mobile- and computer-assistive technologies can …

The Assessment Of Protective Behavioral Strategies: Comparing The Absolute Frequency And Contingent Frequency Response Scales, Benjamin A. Kite, Matthew R. Pearson, James M. Henson

Psychology Faculty Publications

The purpose of the present studies was to examine the effects of response scale on the observed relationships between protective behavioral strategies (PBS) measures and alcohol-related outcomes. We reasoned that an "absolute frequency" scale (stem: "how many times..."; response scale: 0 times to 11 + times) conflates the frequency of using PBS with the frequency of consuming alcohol; thus, we hypothesized that the use of an absolute frequency response scale would result in positive relationships between types of PBS and alcohol-related outcomes. Alternatively, a "contingent frequency" scale (stem: "When drinking ... how often ..."; response scale: never to always …

Daily Use Of Protective Behavioral Strategies And Alcohol-Related Outcomes Among College Students, Matthew R. Pearson, Gabrielle M. D'Lima, Michelle L. Kelley

Psychology Faculty Publications

The purpose of the present study was to examine associations between use of protective behavioral strategies (PBS) and alcohol-related outcomes (alcohol consumption, negative alcohol consequences, and positive alcohol consequences) using a daily diary approach. This approach is less affected by retrospective memory biases than typical self-reports of alcohol-related variables and allows the examination of both between-subjects and within-person effects. Using hierarchical linear modeling of data from 40 subjects who completed daily dairies for up to 15 days, we found significant within-person variation in PBS use over time, and each type of PBS had unique relationships with alcohol-related outcomes. For example, …

Commentary On "The Motor Learning Strategy Instrument: Interrater Reliability Within Usual And Virtual Reality Physical Therapy Interventions", Karen M. Kott, Sharon Held

Rehabilitation Sciences Faculty Publications

No abstract provided.

Effectiveness Of Low-Level Laser Therapy Combined With An Exercise Program To Reduce Pain And Increase Function In Adults With Shoulder Pain: A Critically Appraised Topic, Aimee L. Thornton, Cailee W. Mccarty, Mollie-Jean Burgess

Rehabilitation Sciences Faculty Publications

Clinical Scenario: Shoulder pain is a common musculoskeletal condition that affects up to 25% of the general population. Shoulder pain can be caused by any number of underlying conditions including subacromial impingement syndrome, rotator-cuff tendinitis, and biceps tendinitis. Regardless of the specific pathology, pain is generally the number 1 symptom associated with shoulder injuries and can severely affect daily activities and quality of life of patients with these conditions. Two of the primary goals in the treatment of these conditions are reducing pain and increasing shoulder range of motion (ROM).(3) Conservative treatment has traditionally included a therapeutic exercise program targeted …

Changes In Lower Extremity Biomechanics Due To A Short-Term Fatigue Protocol, Nelson Cortes, Eric Greska, Roger Kollack, Jatin Ambegaonkar, James A. Onate

Human Movement Sciences & Special Education Faculty Publications

Context: Noncontact anterior cruciate ligament injury has been reported to occur during the later stages of a game when fatigue is most likely present. Few researchers have focused on progressive changes in lower extremity biomechanics that occur throughout fatiguing.

Objective: To evaluate the effects of a sequential fatigue protocol on lower extremity biomechanics during a sidestep-cutting task (SS).

Design: Controlled laboratory study.

Setting: Laboratory.

Patients or Other Participants: Eighteen uninjured female collegiate soccer players (age 19.2 ± 0.9 years, height = 1.66 ± 0.5 m, mass 61.6 ± 5.1 kg) volunteered.

Intervention(s): The independent variable was fatigue level, with 3 …

Proceed To Checkout? The Impact Of Time In Advanced Ticket Purchase Decisions, Brendan Dwyer, Joris Drayer, Stephen L. Shapiro

Human Movement Sciences & Special Education Faculty Publications

When purchasing tickets in advance, sports consumers are often faced with uncertainty. Most notably, in today's real-time environment, it can be challenging for consumers to determine how ticket prices and seat availability will change over time. Guided by the generic advanced-booking decision model, the current study investigated the role of time, ticket source (primary or secondary market), and team identification in advanced ticket purchasing by exploring a consumer's perceptions of ticket availability and finding a lower price. The results suggest the perceived likelihood of ticket availability and finding a lower priced ticket increased as the date of the game drew …

The Resiliency And Attitudes Skills Profile: An Assessment Of Factor Structure, Takeyra Williams, Edwin Gomez, Eddie Hill, Tammi Milliken, Jennifer Goff, Nicole Gregory

Human Movement Sciences & Special Education Faculty Publications

This study examines the Resiliency Attitudes and Skills Profile (RASP) in an afterschool recreation program with sixth grade students (N=137). The purpose of the study was to inspect the psychometric properties of the RASP and the internal consistency of the RASP global scale and subscales. This article expands on research reported on the RASP. Findings suggest that the RASP measures aspects of resiliency along four factors/dimensions. Suggestions for future research are provided.

Melatonin Supplementation Improves Glycemic Control While Lowering Oxidative Stress In Type 2 Diabetes, Carmine R. Grieco, Sheri R. Colberg, C. Thomas Somma, Andrew G. Thompson, Aaron I. Vinik

Human Movement Sciences & Special Education Faculty Publications

The purpose of this investigation was to evaluate the effect of melatonin on glycemic control and oxidative stress (OS) in adults with type 2 diabetes (T2D). Fourteen subjects with T2D (10 female, 4 male; 52.5 ± 5.0 years) were randomly assigned to melatonin (MEL) or placebo groups (PLA) for 42 days, in a crossover design. Subjects ingested 10 mg of MEL or an identical placebo (PLA) 30 minutes prior to sleep. Fasting blood draws occurred at baseline, 42 days, and 84 days. Plasma malondialdehyde, a marker of OS, significantly decreased on MEL (-6.25±2.10 nmol/ml) compared to PLA (0.72±3.30, p=0.028). The …

The Big Blue Test: Effects Of 14 Minutes Of Physical Activity On Blood Glucose Levels, Sheri R. Colberg, Manuel J. Hernandez

Human Movement Sciences & Special Education Faculty Publications

[First paragraph] For most with diabetes, physical activity has a blood glucose lowering effect (1). Hypoglycemia (i.e., blood glucose < 65 mg/dL) resulting from exercise is a concern, particularly for insulin users (2).

Blood Glucose Responses To Type, Intensity, Duration, And Timing Of Exercise, Sheri R. Colberg, Manuel J. Hernandez, Fatima Shahzad

Human Movement Sciences & Special Education Faculty Publications

[First Paragraph] The Big Blue Test (BBT) is an annual initiative by the Diabetes Hands Foundation to raise awareness of the importance of physical activity in managing diabetes. Individuals with diabetes voluntarily exercise and record self-monitored blood glucose levels. During the 2012 BBT, 5,157 diabetic participants (~90% insulin users) anonymously entered exercise type, intensity, duration, time elapsed since last meal, and blood glucose readings before and after one or more bouts of exercise separately through www.BigBlueTest.org or an Iphone app.

Development And Validation Of A Predictive Model Of Acute Glucose Response To Exercise In Individuals With Type 2 Diabetes, Bryan S. Gibson, Sheri R. Colberg, Paul Poirier, Denise Maria Martins Vancea, Jason Jones, Robin Marcus

Human Movement Sciences & Special Education Faculty Publications

Background: Our purpose was to develop and test a predictive model of the acute glucose response to exercise in individuals with type 2 diabetes.

Design and methods: Data from three previous exercise studies (56 subjects, 488 exercise sessions) were combined and used as a development dataset. A mixed-effects Least Absolute Shrinkage Selection Operator (LASSO) was used to select predictors among 12 potential predictors. Tests of the relative importance of each predictor were conducted using the Lindemann Merenda and Gold (LMG) algorithm. Model structure was tested using likelihood ratio tests. Model accuracy in the development dataset was assessed by leave-one-out cross-validation. …

Urban Adventure Racing: Using Grounded Theory To Assess Motives, Eddie Hill, Edwin Gómez, Brandi Brinkley, Marni Goldenberg

Human Movement Sciences & Special Education Faculty Publications

Although urban adventure races (ARs) have grown in popularity, little research exists on the reasons for the rising interest. Typically, adventure races are defined as a series of outdoor tasks completed within a given course or timeframe that are meant to challenge individuals, both mentally and physically. ARs borrow much from adventure programming. Aspects of AR programming may include goal-setting, problem-solving activities, and processing, and is often theoretically driven. This study used Grounded Theory as a basis for exploring why participants choose ARs, and if motives vary by gender. Of the 60 questionnaires collected, 40 were suitable for data analysis. …

Prescribing Physical Activity To Prevent And Manage Gestational Diabetes, Sheri R. Colberg, Kristin Castorino, Lois Jovanovič

Human Movement Sciences & Special Education Faculty Publications

Gestational diabetes mellitus (GDM) is the most prevalent metabolic disorder during pregnancy. Women diagnosed with GDM have a substantially greater risk of developing type 2 diabetes within 5-10 years after delivery, and the risk is increased by excess body weight. Uncontrolled hyperglycemia during pregnancy is potentially harmful to both mother and fetus, resulting in a greater need for Caesarian-section deliveries, delivery of larger infants with more excess body fat, a greater risk of infant death and stillbirth, and an elevated risk of infant hypoglycemia immediately after birth. Fortunately, engaging in physical activity prior to and during pregnancy may lower the …

Lead-Based Paint Awareness, Work Practices, And Compliance During Residential Construction And Renovation, James D. Blando, Nickita Antoine, Daniel Lefkowitz

Community & Environmental Health Faculty Publications

The U.S. Environmental Protection Agency recently implemented the Renovation, Repair and Painting (RRP) rule that applies to pre-1978 residences because of the potential presence of lead-based paint. Enforcement of this rule may be difficult and therefore it is crucial to understand the awareness and beliefs of contractors and the general public because these will likely be major determinants of exposures resulting from residential renovation work. The study described in this article utilized two mailed surveys: one directed to the general public and the other directed to contractors. The surveys were conducted in New Jersey and Virginia. Field observations were also …

Nocturnal Diaphoresis Secondary To Mild Obstructive Sleep Apnea In A Patient With A History Of Two Malignancies, Robert Daniel Vorona, Mariana Szklo-Coxe, Mark Fleming, J. Catesby Ware

Community & Environmental Health Faculty Publications

Numerous medical disorders, including obstructive sleep apnea, may cause nocturnal diaphoresis. Previous work has associated severe obstructive sleep apnea with nocturnal diaphoresis. This case report is of import as our patient with severe nocturnal diaphoresis manifested only mild sleep apnea, and, for years, his nocturnal diaphoresis was ascribed to other causes, i.e., first prostate cancer and then follicular B-cell lymphoma. Additionally, it was the nocturnal diaphoresis and not more common symptoms of obstructive sleep apnea, such as snoring, that led to the definitive diagnosis of his sleep apnea and then to treatment with a gratifying resolution of his onerous symptom.