Medicine and Health Sciences Commons^™

Storage Conditions And Passages Alter Il-6 Secretion In C26 Adenocarcinoma Cell Lines, Diana M. Norden, Raymond Devine, Donna O. Mccarthy, Loren E. Wold

College of Nursing Faculty Research and Publications

The C26 adenocarcinoma tumor line is frequently used to establish peripheral tumors in mice for the study of cancer cachexia and cancer-related fatigue. Recently, we have noticed a progressive decline in the effects of tumor growth on our biological and behavioral measures in the tumor-bearing mice. Therefore, we compared effects of three aliquots of the C26 tumor cell line that differed in storage condition and number of passages on cytokine secretion, tumor growth, weight loss and fatigue behavior. Three aliquots of the C26 tumor line were selected as alpha (α), beta (β), and gamma (γ). Aliquot α was an original …

Expression Patterns Of Bdnf With Central Anorexigenic Signaling Pathways Involving Pacap In The Hypothalamic Ventromedial Nuclei, Brian Maunze, Matthew M. Hurley, Jon M. Resch, M. J. Reilley, E. M. Wassmann, Sujean Choi

Biomedical Sciences Faculty Research and Publications

Pituitary adenylate cyclase-activating polypeptide (PACAP) is a 38-amino acid polypeptide belonging to the secretin super family of peptides. PACAP binds to its type 1 receptor (PAC1R) with greater affinity than for the receptors for vasoactive intestinal polypeptides (VIP), VPAC1 and VPAC2. Although mRNA for PACAP and its receptor PAC1R are found throughout the central nervous system, they are abundantly expressed in the hypothalamic ventromedial nuclei (VMN). In male Sprague Dawley rats, infusions of PACAP into the VMN produce a robust decrease in food intake with concomitant increased energy expenditure, decreased body weight, and significantly elevated brain-derived neurotrophic factor (BDNF) mRNA …

Patterns Of Barbell Acceleration During The Snatch In Weightlifting Competition, Kristof Kipp, Chad Harris

Exercise Science Faculty Research and Publications

The purpose of this study was to determine the association between weightlifting performance and vertical barbell acceleration patterns. Barbell kinematic time-series data were tracked from 18 snatches from six weightlifters during a regional weightlifting competition. These data were used to calculate vertical barbell accelerations. Time-series data were normalised to 100% of lift phase, defined as the time interval between barbell lift-off and maximum height of the barbell during each snatch lift. The time-series data were then entered into a pattern recognition algorithm that extracted principal patterns and calculated principal pattern scores. Body mass-normalised lift weight, which was used to quantify …

Conditioned Pain Modulation Predicts Exercise-Induced Hypoalgesia In Healthy Adults, Kathy J. Lemley, Sandra K. Hunter, Marie K. Hoeger Bement

Exercise Science Faculty Research and Publications

Introduction: Conditioned pain modulation (CPM) is the concept that pain inhibits pain and has potential rehabilitation implications for exercise prescription. The purpose of this study was to determine whether changes in pressure pain perception after a thermal conditioning stimulus (i.e., CPM) was attenuated with aging and whether CPM predicted pain relief after exercise (exercise-induced hypoalgesia (EIH)) in healthy young and older adults.

Methods: Twenty young (21.9 ± 3.3 yr, 10 men) and 19 older (72.0 ± 4.5 yr, 10 men) adults participated in three sessions: one familiarization and two experimental (EIH and CPM) sessions. Pressure pain perception was assessed using …

Citrate Anticoagulation: Are Blood Donors Donating Bone?, Walter Bialkowski, Roberta Bruhn, Gustaf Edgren, Paula E. Papanek

Exercise Science Faculty Research and Publications

An estimated 2.4 million volunteer apheresis blood donation procedures were performed in the United States in 2010, and increases in the proportion of transfused blood products derived from apheresis blood collections have been consistently reported. Anticoagulation is required during apheresis and is achieved with citrate. Donor exposure to citrate causes an acute physiological response to maintain serum mineral homeostasis. Some data are available on the sequelae of this acute response in the days and weeks following exposure, raising questions about bone mineral density in regular apheresis donors. New research is emerging that addresses the potential long-term health outcomes of repeated …

Evidence For Respiratory Neuromodulator Interdependence After Cholinergic Disruption In The Ventral Respiratory Column, Clarissa Muere, Suzanne Neumueller, Justin Robert Miller, Samantha Olesiak, Matthew Hodges, Lawrence Pan, Hubert Forster

Physical Therapy Faculty Research and Publications

Reverse dialysis of the muscarinic receptor antagonist, atropine (ATR, 50 mM), into the pre-Bötzinger Complex region of the ventral respiratory column (VRC) of awake and sleeping goats increases breathing frequency and serotonin (5-HT), substance P (SP), glycine, and GABA concentrations in the effluent dialysate. Herein, we report data from goats in which we reverse dialyzed 5 mM ATR or specific antagonists of M2 or M3 muscarinic receptors into the VRC. The effects on frequency of all three antagonists were not significantly different from time control studies. 5 mM ATR and the M3 antagonist increased SP sevenfold less than 50 mM …

A New Obesity Model Reveals The Hypophagic Properties Of Pacap Involve The Regulation Of Homeostatic Feeding In The Ventromedial Hypothalamic Nucleus And Hedonic Feeding In The Nucleus Accumbens, Matthew M. Hurley, Brian Maunze, Jon M. Resch, Mogen M. Frenkel, M. J. Reilley, Megan E. Block, David A. Baker, Sujean Choi

Biomedical Sciences Faculty Research and Publications

Binge eating in humans is a complex disorder that often involves discrete, compulsive feeding sessions of highly palatable foods even in the absence of a deprivation state or hunger. Binging can be effectively modeled in rodents by providing subjects with limited access to a palatable food source (Western Diet; WD) as an adjunct to ad lib access to normal chow (Standard Chow; SC). Although this design recapitulates several fundamental characteristics observed in binge eating disorder, the binge eating observed in this paradigm is likely a product of both hedonic and homeostatic drives with the need to balance energy stores still …

Evaluation Of Pediatric Manual Wheelchair Mobility Using Advanced Biomechanical Methods, Brooke A. Slavens, Alyssa J. Schnorenberg, Christine M. Aurit, Adam Graf, Joseph J. Krzak, Kathryn Reiners, Lawrence C. Vogel, Gerald F. Harris

Biomedical Engineering Faculty Research and Publications

There is minimal research of upper extremity joint dynamics during pediatric wheelchair mobility despite the large number of children using manual wheelchairs. Special concern arises with the pediatric population, particularly in regard to the longer duration of wheelchair use, joint integrity, participation and community integration, and transitional care into adulthood. This study seeks to provide evaluation methods for characterizing the biomechanics of wheelchair use by children with spinal cord injury (SCI). Twelve subjects with SCI underwent motion analysis while they propelled their wheelchair at a self-selected speed and propulsion pattern. Upper extremity joint kinematics, forces, and moments were computed using …

Adamts-4 And Adamts-5 Expression In Human Temporomandibular Joint Discs With Internal Derangement, Correlates With Degeneration, Rosalia Leonardi, Salvatore Crimi, Luis Eduardo Almeida, Giuseppe Pannone, Giuseppe Musumeci, Sergio Castorina, Mugurel Constantin Rusu, Carla Loreto

School of Dentistry Faculty Research and Publications

Temporomandibular joint (TMJ) internal derangement (ID) is one of the most common form of temporomandibular disorders. There is evidence showing the increased expression of matrix metalloproteinases (MMPs) in the cells from degenerated TMJ disc. ADAMTS are a large family of metalloproteases which are responsible for proteoglycans degradation. The present study aimed to evaluate ADAMTS-4 and ADAMTS-5 immunohistochemical expression in human TMJ discs from patients affected by ID, and to find out if there is any correlation with the degree of histopathological changes. Eighteen temporomandibular displaced disc specimens and sixteen TMJ disc control were used for the present study. Specimens were …

Investigation Of Force Decay In Aesthetic, Fibre-Reinforced Composite Orthodontic Archwires, Jacob E. Spendlove, David W. Berzins, Jessica E. Pruszynski, Richard W. Ballard

School of Dentistry Faculty Research and Publications

Background/Objectives: Because polymer-based materials typically exhibit viscoelastic properties, the objective was to determine if commercially available, aesthetic, fibre-reinforced composite archwires maintain continuous forces without undergoing force decay when deflected continuously.

Materials/Methods: Quasi force decay was evaluated by comparing three-point bending profiles of nickel–titanium (NiTi) and fibre-reinforced composite archwires (BioMers) prior to and after 30 days of continuous deflection of either 1 or 2mm. Paired t-tests or non-parametric signed rank tests were used to statistically compare pre- and post-deflection bending forces. A control group consisting of wires not subject to the 30-day constant deflection was tested to check whether the initial …

Morphologic Outcome Of Bimaxillary Surgery–An Anthropometric Appraisal, Gregor F. Raschke, Ulrich M. Rieger, Andre Peisker, Gabriel Djedovic, Marta Gomez-Dammeier, Arndt Guentsch, Oliver Schaefer, Stefan Schultze-Mosgau

School of Dentistry Faculty Research and Publications

Objectives: To adequately perform orthognathic surgery procedures, it is from basic interest to understand the morphologic changes caused by orthognathic surgery. Anthropometric analyses of standardized frontal view and profile photographs could help to investigate and understand such changes. Study Design: We present a pre- to postoperative evaluation of orthognathic surgery results based on anthropometric indices described by Farkas and cephalometric measurements. 30 Class III patients undergoing maxillary advancement by Le Fort I Osteotomy and mandibular setback by bilateral sagittal split osteotomy were evaluated. Preoperative as well as three and nine months postoperative lateral cephalograms as well as standardized frontal view …

Developing Student, Family, And School Constructs From Nlts2 Data, Karrie A. Shogren, Mauricio Garnier-Villarreal

School of Dentistry Faculty Research and Publications

The purpose of this study was to use data from the National Longitudinal Transition Study–2 (NLTS2) to (a) conceptually identify and empirically establish student, family, and school constructs; (b) explore the degree to which the constructs can be measured equivalently across disability groups; and (c) examine latent differences (means, variances, and correlations) in the constructs across disability groups. Conceptual analysis of NLTS2 individual survey items yielded 21 student, family, and school constructs, and 16 were empirically supported. Partial strong metric invariance was established across disability groups, and in the latent space, a complex pattern of mean and variance differences across …

Erupted Complex Odontoma Mimicking A Mandibular Second Molar, Luis Eduardo Almeida, M. O. Andrade, M. A. De Oliveira Filho, Paula C. Trevilatto, Andrea Doetzer

School of Dentistry Faculty Research and Publications

Complex odontoma (CO) is considered one of the most common odontogenic lesions, composed by a miscellaneous of dental tissue such as enamel, dentin, pulp and sometimes cementum. They may interfere with the eruption of an associated tooth, being more prevalent in the posterior mandible. CO has been rarely reported as erupted, being considered an intraosseous lesion. This is a case report of a 17-year-old male with a benign fibro-osseous lesion consistent with CO that was located at the left second molar region, above the crown of the impacted mandibular second molar tooth. The lesion was surgically removed, and the tooth …

Effect Of Additives On Mineral Trioxide Aggregate Setting Reaction Product Formation, Angela M. Zapf, Sharath C.V. Chedella, David W. Berzins

School of Dentistry Faculty Research and Publications

Introduction

Mineral trioxide aggregate (MTA) sets via hydration of calcium silicates to yield calcium silicate hydrates and calcium hydroxide (Ca[OH]₂). However, a drawback of MTA is its long setting time. Therefore, many additives have been suggested to reduce the setting time. The effect those additives have on setting reaction product formation has been ignored. The objective was to examine the effect additives have on MTA's setting time and setting reaction using differential scanning calorimetry (DSC).

Methods

MTA powder was prepared with distilled water (control), phosphate buffered saline, 5% calcium chloride (CaCl₂), 3% sodium hypochlorite (NaOCl), or …

Behavioral Assessment Of Acute Inhibition Of System XC - In Rats, Victoria Lutgen, Jon M. Resch, Krista Qualmann, Nicholas J. Raddatz, Cristina Panhans, Ellen M. Olander, Linghai Kong, Sujean Choi, John R. Mantsch, David A. Baker

Biomedical Sciences Faculty Research and Publications

Rationale

Gaps in our understanding of glutamatergic signaling may be key obstacles in accurately modeling complex CNS diseases. System x_c ^- is an example of a poorly understood component of glutamate homeostasis that has the potential to contribute to CNS diseases.

Objectives

This study aims to determine whether system x_c ^- contributes to behaviors used to model features of CNS disease states.

Methods

In situ hybridization was used to map mRNA expression of xCT throughout the brain. Microdialysis in the prefrontal cortex was used to sample extracellular glutamate levels; HPLC was used to measure extracellular glutamate and tissue …

Augmented Cystine–Glutamate Exchange By Pituitary Adenylate Cyclase-Activating Polypeptide Signaling Via The Vpac1 Receptor, Jon M. Resch, Rebecca Albano, Xiaoqian Liu, Julie Hjelmhaug, Doug Lobner, David A. Baker, Sujean Choi

Biomedical Sciences Faculty Research and Publications

In the central nervous system, cystine import in exchange for glutamate through system x_c^- is critical for the production of the antioxidant glutathione by astrocytes, as well as the maintenance of extracellular glutamate. Therefore, regulation of system x_c^- activity affects multiple aspects of cellular physiology and may contribute to disease states. Pituitary adenylate cyclase-activating polypeptide (PACAP) is a neuronally derived peptide that has already been demonstrated to modulate multiple aspects of glutamate signaling suggesting PACAP may also target activity of cystine–glutamate exchange via system x_c^-. In this study, 24-h treatment of primary cortical …

Chronic Conditions In Elders In Assisted Living Facilities: Associations With Daily Functioning, Self-Assessed Health, And Depressive Symptoms, Abir K. Bekhet, Jaclene A. Zauszniewski

College of Nursing Faculty Research and Publications

Background

The increasing life expectancy of older adults has prompted an increase in chronic conditions that may interfere with their daily living and impact physical and mental health.

Objectives

This study examined associations between commonly reported chronic conditions, daily functioning, self-assessed health, and depressive symptoms of elders.

Methods/Design

A secondary analysis of existing data from 314 elderly residents of 29 facilities was conducted.

Results

The most frequently reported conditions were arthritis (64%), hypertension (47%), and heart problems (35%). Elders who reported having all three of these most frequently reported conditions differed significantly from those who reported none or one of …

Nurse Value-Added And Patient Outcomes In Acute Care, Olga Yakusheva, Richard C. Lindrooth, Marianne E. Weiss

College of Nursing Faculty Research and Publications

Objective

The aims of the study were to (1) estimate the relative nurse effectiveness, or individual nurse value-added (NVA), to patients’ clinical condition change during hospitalization; (2) examine nurse characteristics contributing to NVA; and (3) estimate the contribution of value-added nursing care to patient outcomes.

Data Sources/Study Setting

Electronic data on 1,203 staff nurses matched with 7,318 adult medical–surgical patients discharged between July 1, 2011 and December 31, 2011 from an urban Magnet-designated, 854-bed teaching hospital.

Study Design

Retrospective observational longitudinal analysis using a covariate-adjustment value-added model with nurse fixed effects.

Data Collection/Extraction Methods

Data were extracted from the study …

Gender Differences In Neighborhood Walking In Older Adults, N. A. Gallagher, P. J. Clarke, Kimberlee A. Gretebeck

College of Nursing Faculty Research and Publications

Objective: This study examined mobility, self-efficacy, outcome expectations, neighborhood (density, destinations, and design), and neighborhood walking in older men (n = 106, 60-99 years, M = 76.78, SD = 8.12) and women (n = 216, 60-99 years, M = 75.81, SD = 8.46).

Method/Results: In hierarchical regression, the variables explained 32% of the variance in neighborhood walking in men (p < .001) and 27% of the variance in women (p < .01). Self-efficacy (β = .49, p < .01), density (β= .22, p < .05), and design (β= .21, p = .05) were associated with walking in men. Significant design characteristics included sidewalks (β= .25, p < .05) and crime (β= .36, p < .01). In women, self-efficacy (β= .48, p < .001) and destinations (β= .15, p < .05) were associated with walking. Walking was associated with self-efficacy for walking despite individual barriers in women (β= .38, p < .001) and neighborhood barriers in men (β= .30, p < .05).

Conclusion: Walking interventions targeting older women should incorporate local destinations. In older …

Phosphate And Acidosis Act Synergistically To Depress Peak Power In Rat Muscle Fibers, Cassandra R. Nelson, Edward P. Debold, Robert H. Fitts

Biomedical Sciences Faculty Research and Publications

Skeletal muscle fatigue is characterized by the buildup of H⁺ and inorganic phosphate (P_i), metabolites that are thought to cause fatigue by inhibiting muscle force, velocity, and power. While the individual effects of elevated H⁺ or P_i have been well characterized, the effects of simultaneously elevating the ions, as occurs during fatigue in vivo, are still poorly understood. To address this, we exposed slow and fast rat skinned muscle fibers to fatiguing levels of H⁺ (pH 6.2) and P_i (30 mM) and determined the effects on contractile properties. At 30°C, elevated P_{i …}

Effect Of A Social Script Ipad Application For Children With Autism Going To Imaging, Norah L. Johnson, Octavia Bree, Erin E. Lalley, Kelly Rettler, Pam Grande, Md O. Gani, Sheikh Iqbal Ahamed

College of Nursing Faculty Research and Publications

This randomized controlled trial feasibility study tested the effectiveness of an iPad® application (app) social script intervention for children with autism spectrum disorder (ASD) going to imaging and their parent (n = 32 parent/child dyads). Parents of the children exposed to the app (n = 16) had lower state anxiety compared to the parents whose children were not exposed to the app (n = 16) (effect size 0.33). Children exposed to the app had fewer externalized challenging behaviors than the control group (effect size 0.56). The results demonstrate feasibility and efficacy of the intervention. Further study of …

An Emerging Population: The Chronically Critically Ill, Philip Jurasinski, Christine A. Schindler

College of Nursing Faculty Research and Publications

No abstract provided.

Access Site Complications After Peripheral Vascular Interventions: Incidence, Predictors, And Outcomes, Daniel Ortiz, Arshad Jahangir, Maharaj Singh, Suhail Allaqaband, Tanvir Bajwa, Mark W. Mewissen

College of Nursing Faculty Research and Publications

Background—

Access site hematomas and pseudoaneurysms are the most frequent complications of peripheral vascular intervention (PVI); however, their incidence and risk factors remain unclear.

Methods and Results—

We retrospectively analyzed data from the multicenter Vascular Quality Initiative on 22 226 patients who underwent 27 048 PVI from August 2007 to May 2013. Primary end points included incidence and predictors of access site complications (ASCs), length of postprocedural hospitalization, discharge status, and 30-day and 1-year mortality. ASC complicated 936 procedures (3.5%). Of these, 74.4% were minor complications, 9.7% were moderate requiring transfusion, 5.4% were moderate requiring thrombin injection, and 10.5% were …

Effects Of Sex And Gender On Adaptation To Space: Musculoskeletal Health, Lori Ploutz-Snyder, Susan Bloomfield, Scott M. Smith, Sandra K. Hunter, Kim Templeton, Debra Bemben

Exercise Science Faculty Research and Publications

There is considerable variability among individuals in musculoskeletal response to long-duration spaceflight. The specific origin of the individual variability is unknown but is almost certainly influenced by the details of other mission conditions such as individual differences in exercise countermeasures, particularly intensity of exercise, dietary intake, medication use, stress, sleep, psychological profiles, and actual mission task demands. In addition to variations in mission conditions, genetic differences may account for some aspect of individual variability. Generally, this individual variability exceeds the variability between sexes that adds to the complexity of understanding sex differences alone. Research specifically related to sex differences of …

The Economic Costs And Benefits Of Dental Education: An Empirical Analysis, Gary L. Stafford, Farrokh Nourzad, William K. Lobb, Jason R. Beall

School of Dentistry Faculty Research and Publications

The rising costs associated with obtaining a dental education have caused some to question the financial benefit of pursuing a dental degree. There is a concern that recent graduates may have difficulty finding professional opportunities that provide the income necessary to service their accumulated educational debt. The aim of this study was to evaluate the trends in educational costs to aid in making an accurate appraisal of the financial benefit of a dental education. Adjusted into constant dollar terms, data from a variety of sources were collected for economic variables such as tuition, fees, student indebtedness, and dentists’ earnings. These …

Christina Nelson - Applying John Hagan’S Power-Control Theory To Gender Differences With Drug Abuse, Christina Nelson

Ronald E. McNair Scholars Program 2014

Previous work has indicated that young men and women engage in drug use in different ways and for different reasons. This research tests Hagan’s Power-Control Theory to examine gender differences in adolescent drug use. This study analyzes randomly selected subsample of data from the National Survey on Drug Use and Health (NSDUH). Questions that led this study were: Do patterns of drug abuse differ by gender? To what extent can Hagan’s Power-Control Theory explain this? Control variables looked at gender, age and race. All adolescents were from the ages of 12-17. Dependent variables looked at drug use (ever, yearly, monthly). …

Brittany Bernal - Sensorimotor Adaptation Of Speech Through A Virtually Shortened Vocal Tract, Brittany A. Bernal

Ronald E. McNair Scholars Program 2014

The broad objective of this line of research is to understand how auditory feedback manipulations may be used to elicit involuntary changes in speech articulation. We examine speech sensorimotor adaptation to supplement the development of speech rehabilitation applications that benefit from this learning phenomenon. By manipulating the acoustics of one’s auditory feedback, it is possible to elicit involuntary changes in speech articulation. We seek to understand how virtually manipulating participants’ perception of vowel space affects their speech movements by assessing acoustic variables such as formant frequency changes. Participants speak through a digital audio processing device that virtually alters the perceived …

Antecedents Of Regular Exercise Among Women Who Do And Do Not Achieve Weight Loss Over Six Months, Heather Vartanian

Dissertations (1934 -)

In the United States, 35.5% of adult women are classified as obese, defined as a body mass index (BMI) of > 30 kg/m2. The health risks associated with obesity can be mitigated through losing even 5-10% of initial body weight. Evidence shows that reductions in caloric intake alone without increases in caloric expenditure leads to a decline in resting metabolic rate, thereby impeding attainment or maintenance of weight loss. The purpose of this cross-sectional, mixed-methods study was to explore the antecedents of regular exercise among women with obesity who did and did not achieve weight loss over six months. The antecedents …

Factors Affecting Disruption In Families Of Adults With Mental Illness, M. Jane Suresky, Jaclene Zauszniewski, Abir K. Bekhet

College of Nursing Faculty Research and Publications

PURPOSE: This study examined relationships between vulnerability/risk and protective factors, and family functioning in women family members of adults with serious mental illness.

DESIGN AND METHODS: Using a descriptive, correlational design, this secondary analysis examined characteristics of the family member with mental illness (e.g., diagnosis, level of care) and measures of caregiver stigma and strain, client dependence, family disruption, sense of coherence, and resourcefulness.

FINDINGS: Family disruption was greatest inwomenwho provided direct care and whose family member had major depression, followed by bipolar disorder, schizophrenia, and panic disorder. Sense of coherence and resourcefulness were associated with lower family disruption, but …

Relationships Among Uncertainty, Coping, And Psychological Distress In Older Adults With Mild Cognitive Impairment, Jennifer Sjostedt Avery

Dissertations (1934 -)

Mild cognitive impairment (MCI) has an average prevalence of 18.9% and most often affects people 60 years of age or older. It is a cognitive stage between normal functioning and dementia (Petersen, 2003; Petersen, 2011; Petersen et al., 2014). MCI can be broken into two subtypes classified by the presence of memory impairment (amnestic MCI) or the lack thereof (nonamnestic MCI). Medical diagnostic criteria are commonly used to guide research with older adults with MCI. A theoretical framework that addresses the antecedents and consequences of MCI, specifically one examining the relationships among MCI, uncertainty, coping and psychological distress, is essential …