Medicine and Health Sciences Commons^™

Powering Single-Cell Genomics To Unravel Circulating Tumour Cell Subpopulations In Non-Small Cell Lung Cancer Patients, Emmanuel Acheampong, Michael Morici, Afaf Abed, Samantha Bowyer, Du-Bois Asante, Weitao Lin, Michael Millward, Elin S. Gray, Aaron Beasley

Research outputs 2022 to 2026

Background

Circulating tumour cells (CTCs) are attractive “liquid biopsy” candidates that could provide insights into the different phenotypes of tumours present within a patient. The epithelial-to-mesenchymal transition (EMT) of CTCs is considered a critical step in tumour metastasis; however, it may confound traditional epithelial feature-based CTC isolation and detection. We applied single-cell copy number alteration (CNA) analysis for the identification of genomic alterations to confirm the neoplastic nature of circulating cells with only mesenchymal phenotypes.

Methods

We isolated CTCs from blood samples collected from 46 NSCLC patients using the Parsortix system. Enriched cells were subjected to immunofluorescent staining for CTC …

Impact Of Converging Sociocultural And Substance-Related Trends On Us Autism Rates: Combined Geospatiotemporal And Causal Inferential Analysis, Albert Stuart Reece, Gary Kenneth Hulse

Research outputs 2022 to 2026

Whilst cannabis is known to be toxic to brain development, it is unknown if it is driving rising US autism rates (ASMR). A longitudinal epidemiological study was conducted using national autism census data from the US Department of Education Individuals with Disabilities Act (IDEA) 1991–2011 and nationally representative drug exposure (cigarettes, alcohol, analgesic, and cocaine abuse, and cannabis use monthly, daily, and in pregnancy) datasets from National Survey of Drug Use and Health and US Census (income and ethnicity) and CDC Wonder population and birth data. Analysis was conducted in R. 266,950 were autistic of a population of 40,119,464 8-year-olds …

Integrative Review: Factors Impacting Effective Delegation Practices By Registered Nurses To Assistants In Nursing, Carol Crevacore, Elisabeth R. Jacob, Linda L. Coventry, Christine Duffield

Research outputs 2022 to 2026

Aim

To identify the evidence on factors that impact delegation practices by Registered Nurses to Assistants in Nursing in acute care hospitals.

Design

An integrative review.

Data Sources

Database searches were conducted between July 2011 and July 2021.

Review Methods

We used the 12-step approach by Kable and colleagues to document the search strategy. The (Whittemore & Knafl. 2005. Journal of Advanced Nursing, 52(5), 546–553) integrative review framework method was adopted and the methodological quality of the studies was assessed using Joanna Briggs critical appraisal instruments.

Results

Nine studies were included. Delegation between the Registered Nurse and the …

Nursing Students Doing Gender: Implications For Higher Education And The Nursing Profession, Lesley J. Andrew, Ken Robinson, Julie Dare, Leesa N. Costello

Research outputs 2022 to 2026

The average age of women nursing students in Australia is rising. With this comes the likelihood that more now begin university with family responsibilities, and with their lives structured by the roles of mother and partner. Women with more traditionally gendered ideas of these roles, such as nurturing others and self-sacrifice, are known to be attracted to nursing as a profession; once at university, however, these students can be vulnerable to gender role stress from the competing demands of study. A qualitative research design, guided by Gadamer's hermeneutic philosophy, explored the gendered behaviours and experiences of 22 women nursing students, …

Covid-19 Lessons To Protect Populations Against Future Pandemics By Implementing Pppm Principles In Healthcare, Cuihong Tian, Lois Balmer, Xuerui Tan

Research outputs 2022 to 2026

The coronavirus disease 2019 (COVID-19) pandemic has continued for more than 3 years, placing a huge burden on society worldwide. Although the World Health Organization (WHO) has declared an end to COVID-19 as a Public Health Emergency of International Concern (PHEIC), it is still considered a global threat. Previously, there has been a long debate as to whether the COVID-19 emergency will eventually end or transform into a more common infectious disease from a PHEIC, and how should countries respond to similar pandemics in the future more time-efficiently and cost-effectively. We reviewed the past, middle and current situation of COVID-19 …

Economic Evaluation Of The Very Early Rehabilitation In Speech (Verse) Intervention, J. Kim, G. Sookram, Erin Godecke, Emily Louise Brogan, Elizabeth Armstrong, F. Ellery, T. Rai, M. L. Rose, Natalie Ciccone, S. Middleton, A. Holland, G. J. Hankey, J. Bernhardt, D. A. Cadilhac

Research outputs 2022 to 2026

Introduction: There is limited evidence on the costs and outcomes of patients with aphasia after stroke. The aim of this study was to estimate costs in patients with aphasia after stroke according to the aphasia therapies provided.

Methods: A three-arm, prospective, randomized, parallel group, open-label, blinded endpoint assessment trial conducted in Australia and New Zealand. Usual ward-based care (Usual Care) was compared to additional usual ward-based therapy (Usual Care Plus) and a prescribed and structured aphasia therapy program in addition to Usual Care (the VERSE intervention). Information about healthcare utilization and productivity were collected to estimate costs in Australian dollars …

Effects Of Submaximal And Supramaximal Accentuated Eccentric Loading On Mass And Function, Sergio Maroto-Izquierdo, Fernando Martín-Rivera, Kazunori Nosaka, Marco Beato, Javier González-Gallego, José A. De Paz

Research outputs 2022 to 2026

Introduction: Eccentric-overload (EO) resistance training emerges as an alternative to more optimally prescribe intensity relative to the force generation capabilities of the eccentric muscle contraction. Given the difficulties to individually prescribe absolute eccentric loads relative to each person’s eccentric ability, setting the load relative to the concentric one-repetition maximum (1-RM) is the most used EO training approach. Therefore, we investigated the effects of submaximal and supramaximal (i.e., eccentric loads above 100% of 1-RM) accentuated eccentric training on changes in lean mass, anabolic hormonal responses and muscle function. Methods: Physically active university students (n = 27) were randomly assigned to two …

Lepidium Meyenii Walp (Maca)-Derived Extracellular Vesicles Ameliorate Depression By Promoting 5-Ht Synthesis Via The Modulation Of Gut–Brain Axis, Rui Hong, Lan Luo, Liang Wang, Zhao-Li Hu, Qi-Rong Yin, Ming Li, Bin Gu, Bin Wang, Tao Zhuang, Xin-Yue Zhang, Yuan Zhou, Wan Wang, Lin-Yan Huang, Bing Gu, Su-Hua Qi

Research outputs 2022 to 2026

Depression is a common and debilitating condition for which effective treatments are needed. Lepidium meyenii Walp (Maca) is a plant with potential medicinal effects in treating depression. Recently, there has been growing interest in plant-derived extracellular vesicles (EVs) due to their low toxicity and ability to transport to human cells. Targeting the gut–brain axis, a novel strategy for depression management, may be achieved through the use of Maca-derived EVs (Maca-EVs). In this study, we successfully isolated Maca-EVs using gradient ultracentrifugation and characterized their shape, size, and markers (CD63 and TSG101). The in vivo imaging showed that the Dil-labeled Maca-EVs crossed …

Location Of Pathogenic Variants In Psen1 Impacts Progression Of Cognitive, Clinical, And Neurodegenerative Measures In Autosomal-Dominant Alzheimer's Disease, Stephanie A. Schultz, Zahra Shirzadi, Aaron P. Schultz, Lei Liu, Colleen D. Fitzpatrick, Eric Mcdade, Nicolas R. Barthelemy, Alan Renton, Bianca Esposito, Nelly Joseph-Mathurin, Carlos Cruchaga, Charles D. Chen, Alison Goate, Ricardo F. Allegri, Tammie L. S. Benzinger, Sarah Berman, Helena C. Chui, Anne M. Fagan, Martin R. Farlow, Nick C. Fox, Brian A. Gordon, Gregory S. Day, Neill R. Graff-Radford, Jason J. Hassenstab, Bernard J. Hanseeuw, Anna Hofmann, Clifford R. Jack Jr, Mathias Jucker, Celeste M. Karch, Robert A. Koeppe, Jae-Hong Lee, Allan I. Levey, Johannes Levin, Ralph Martins, Hiroshi Mori, John C. Morris, James Noble, Richard J. Perrin, Pedro Rosa-Neto, Stephen P. Salloway, Raquel Sanchez-Valle, Peter R. Schofield, Chengjie Xiong, Keith A. Johnson, Randall J. Bateman, Reisa A. Sperling, Jasmeer P. Chhatwal, Dominantly Inherited Alzheimer Network Investigators

Research outputs 2022 to 2026

Although pathogenic variants in PSEN1 leading to autosomal-dominant Alzheimer disease (ADAD) are highly penetrant, substantial interindividual variability in the rates of cognitive decline and biomarker change are observed in ADAD. We hypothesized that this interindividual variability may be associated with the location of the pathogenic variant within PSEN1. PSEN1 pathogenic variant carriers participating in the Dominantly Inherited Alzheimer Network (DIAN) observational study were grouped based on whether the underlying variant affects a transmembrane (TM) or cytoplasmic (CY) protein domain within PSEN1. CY and TM carriers and variant non-carriers (NC) who completed clinical evaluation, multimodal neuroimaging, and lumbar puncture for collection …

Men And Women Differ In Their Interest And Willingness To Participate In Exercise And Sports Science Research, James L. Nuzzo, Robert O. Deaner

Research outputs 2022 to 2026

Unequal proportions of male and female participants in exercise research might be attributed, in part, to differences in interest and willingness to participate. We tested if men and women are equally interested and willing to undergo exercise research procedures and if they consider different factors when deciding to participate. Two samples completed an online survey. Sample 1 (129 men, 227 women) responded to advertisements on social media and survey-sharing websites. Sample 2 (155 men, 504 women) was comprised of undergraduate psychology students. In both samples, men were significantly more interested to learn their muscle mass amount, running speed, jump height, …

Patient Navigation Across The Cancer Care Continuum: An Overview Of Systematic Reviews And Emerging Literature, Raymond J. Chan, Vivienne E. Milch, Fiona Crawford-Williams, Oluwaseyifunmi A. Agbejule, Ria Joseph, Jolyn Johal, Narayanee Dick, Matthew P. Wallen, Julie Ratcliffe, Anupriya Agarwal, Larissa Nekhlyudov, Matthew Tieu, Manaf Al-Momani, Scott Turnbull, Rahul Sathiaraj, Dorothy Keefe, Nicolas H. Hart

Research outputs 2022 to 2026

Patient navigation is a strategy for overcoming barriers to reduce disparities and to improve access and outcomes. The aim of this umbrella review was to identify, critically appraise, synthesize, and present the best available evidence to inform policy and planning regarding patient navigation across the cancer continuum. Systematic reviews examining navigation in cancer care were identified in the Cochrane Central Register of Controlled Trials (CENTRAL), PubMed, Embase, Cumulative Index of Nursing and Allied Health (CINAHL), Epistemonikos, and Prospective Register of Systematic Reviews (PROSPERO) databases and in the gray literature from January 1, 2012, to April 19, 2022. Data were screened, …

Time-Out For Well-Being: A Mixed Methods Evaluation Of Attitudes And Likelihood To Engage In Different Types Of Online Emotional Well-Being Programmes In The Perinatal Period, Jacqueline A. Davis, Amy L. Finlay-Jones, Natasha Bear, Susan L. Prescott, Desiree T. Silva, Jeneva L. Ohan

Research outputs 2022 to 2026

Background: Positive maternal mental health during the perinatal period contributes to general well-being and positive emotional bonds with the child, encouraging an optimal developmental trajectory. Online interventions to enhance maternal well-being and develop coping skills, such as meditation-based interventions, can be a low-cost way to improve mother and child outcomes. However, this depends on end-user engagement. To date, there is limited evidence about women’s willingness to engage and preferences for online programmes. Objectives: This study explored pregnant women’s attitudes towards and likelihood to undertake minimal online well-being training programmes (mindfulness, self-compassion, or general relaxation), engagement barriers and enablers, and programme …

Two-Year Prognostic Utility Of Plasma P217+Tau Across The Alzheimer’S Continuum, A. Feizpour, V. Doré, J. D. Doecke, Z. S. Saad, G. Triana-Baltzer, R. Slemmon, P. Maruff, N. Krishnadas, P. Bourgeat, K. Huang, C. Fowler, S. R. Rainey-Smith, A. I. Bush, L. Ward, J. Robertson, R. N. Martins, C. L. Masters, V. L. Villemagne, J. Fripp, H. C. Kolb, C. C. Rowe

Research outputs 2022 to 2026

Background: Plasma p217+tau has shown high concordance with cerebrospinal fluid (CSF) and positron emission tomography (PET) measures of amyloid- (A ) and tau in Alzheimer’s Disease (AD). However, its association with longitudinal cognition and comparative performance to PET A and tau in predicting cognitive decline are unknown. Objectives: To evaluate whether p217+tau can predict the rate of cognitive decline observed over two-year average follow-up and compare this to prediction based on A (18F-NAV4694) and tau (18F-MK6240) PET. We also explored the sample size required to detect a 30% slowing in cognitive decline in a 2-year trial and selection test cost …

Unlocking The Restraint—Development Of A Behaviour Change Intervention To Increase The Provision Of Modified Constraint-Induced Movement Therapy In Stroke Rehabilitation, Ashan Weerakkody, Robyn Emmanuel, Jocelyn White, Erin Godecke, Barby Singer

Research outputs 2022 to 2026

Background: Strong evidence supports the provision of modified constraint-induced movement therapy (mCIMT) to improve upper limb function after stroke. A service audit identified that very few patients received mCIMT in a large subacute, early-supported discharge rehabilitation service. A behaviour change intervention was developed to increase the provision of mCIMT following an unsuccessful ‘education only’ attempt. This paper aims to systematically document the steps undertaken and to provide practical guidance to clinicians and rehabilitation services to implement this complex, yet effective, rehabilitation intervention. Methods: This clinician behaviour change intervention was developed over five stages and led by a working group of …

What Makes A Space Safe? Consumers' Perspectives On A Mental Health Safe Space, Lesley Andrew, Shantha Karthigesu, David Coall, Moira Sim, Julie Dare, Kathy Boxall

Research outputs 2022 to 2026

The provision of community-based space for people experiencing a mental health crisis is regarded as a favourable alternative to the emergency department. However, the only non-emergency department safe spaces in Western Australia are located within hospitals or hospital grounds. This qualitative study asked mental health consumers in Western Australia with experience of presentation at the emergency department during a mental health crisis to describe what a safe space would look and feel like. Data were collected through focus groups and thematically analysed. The findings present the voices of mental health consumers through the framework of health geography and the therapeutic …

Fetal Growth Trajectories And Measures Of Insulin Resistance In Young Adults, Ashish Yadav, Lawrence J. Beilin, Rae-Chi Huang, John P. Newnham, Scott W. White, Trevor A. Mori

Research outputs 2022 to 2026

Context: Events during gestation greatly influence the risk of cardiometabolic diseases including diabetes in offspring during later life. Objective: This study aimed to investigate relationships between serial ultrasound-derived fetal growth trajectories and markers of insulin resistance in young adults in the Raine Study, an Australian pregnancy cohort. Methods: Linear mixed modeling examined the relationship between fetal growth trajectory groups, constructed using serial ultrasound-based abdominal circumference (AC), femur length (FL), and head circumference (HC) from 1333 mother-fetal pairs, and offspring Homeostatic Model Assessment of Insulin Resistance (HOMA-IR), as a marker of diabetes risk, at 20 (n = 414), 22 (n = …

Unrecognized Hypertension Among A General Adult Ghanaian Population: An Urban Community-Based Cross-Sectional Study Of Prevalence And Putative Risk Factors Of Lifestyle And Obesity Indices, Enoch O. Anto, Wina I. O. Boadu, Emmanuel E. Korsah, Ezkiel Ansah, Eric Adua, Joseph Frimpong, Patience Nyarkoa, Valentine C. K. T. Tamakloe, Emmanuel Acheampong, Evans A. Asamoah, Stephen Opoku, Ebenezer Afrifa-Yamoah, Max E. Annani-Akollor, Christian Obirikorang

Research outputs 2022 to 2026

Hypertension (HTN) is the leading cause of cardiovascular diseases. Nevertheless, most individuals in developing countries are unaware of their blood pressure status. We determined the prevalence of unrecognized hypertension and its association with lifestyle factors and new obesity indices among the adult population. This community-based study was conducted among 1288 apparently healthy adults aged 18–80 years in the Ablekuma North Municipality, Ghana. Sociodemographic, lifestyle characteristics, blood pressure and anthropometric indices were obtained. The prevalence of unrecognized HTN was 18.4% (237 / 1288). The age groups 45–54 years [aOR = 2.29, 95% CI (1.33–3.95), p = 0.003] and 55–79 years [aOR …

Utility Of Dna Methylation As A Biomarker In Ageing And Alzheimer’S Disease, Lidija Milicic, Tenielle Porter, Michael Vacher, Simon M. Laws

Research outputs 2022 to 2026

Epigenetic mechanisms such as DNA methylation have been implicated in a number of diseases including cancer, heart disease, autoimmune disorders, and neurodegenerative diseases. While it is recognized that DNA methylation is tissue-specific, a limitation for many studies is the ability to sample the tissue of interest, which is why there is a need for a proxy tissue such as blood, that is reflective of the methylation state of the target tissue. In the last decade, DNA methylation has been utilized in the design of epigenetic clocks, which aim to predict an individual’s biological age based on an algorithmically defined set …

Working Together To Increase Australian Children’S Liking Of Vegetables: A Position Statement By The Vegetable Intake Strategic Alliance (Visa), David N. Cox, Karen J. Campbell, Lynne Cobiac, Claire Gardner, Lucinda Hancock, Gilly Hendrie, Amber Kelaart, Michelle Lausen, Astrid Poelman, Ros Sambell, Kim M. Tikellis, Bonnie Wiggins

Research outputs 2022 to 2026

Children need to be repeatedly and consistently exposed to a variety of vegetables from an early age to achieve an increase in vegetable intake. A focus on enjoyment and learning to like eating vegetables at an early age is critical to forming favourable lifelong eating habits. Coordinated work is needed to ensure vegetables are available and promoted in a range of settings, using evidence-based initiatives, to create an environment that will support children’s acceptance of vegetables. This will help to facilitate increased intake, and ultimately realise the associated health benefits. The challenges and evidence base for a new approach are …

A Scoping Review: Identifying Targeted Intervention Strategies For Workers With Occupational Hearing Loss, Adelle Liebenberg, Melinda Gardner, Valerie M. Nie, Carole L. James, Sue Reed

Research outputs 2022 to 2026

An apparent disconnect exist in workplaces regarding identification of occupational hearing loss (OHL) and implementation of specific strategies to prevent progression of OHL, evident through continued high incidence of OHL. This scoping review aimed to identify evidence regarding targeted intervention used by industry, specifically to prevent the progression of OHL for workers. The scoping review was undertaken using the PRISMA-ScR methodology. Search terms were based on three broad categories, hearing loss, workplace, and intervention. Initially 1309 articles were identified for screening and 1,207 studies not meeting the criteria were excluded. Full text reviews of 102 articles were completed and a …

Bouncing Back From Covid-19: A Western Australian Community Perspective, Kiira K. Sarasjärvi, Paola Chivers, Ranila Bhoyroo, Jim Codde

Research outputs 2022 to 2026

Introduction: This study explored the behavioral profiles of residing Western Australians during a COVID-19 lockdown period and transitions in behavior post-lockdown. Methods: A total of 313 participants (76% female, age: M = 50.1, SD = 15.7 years) completed behavioral and mental health questionnaire items ~2 months after a 3-month COVID-19 lockdown in October 2020, using a retrospective recall to assess their experience during the lockdown period. Latent transition analysis (LTA) was used to identify behavioral profiles and transitions. Indicators were identified by assessing during–post-lockdown group differences (Kruskal–Wallis, chi-square tests) and profiles described using qualitative open-ended questions. Results: Significant indicators included …

Editorial: Sleep, Vigilance & Disruptive Behaviors, Osman S. Ipsiroglu, Gerhard Klösch, Rosalia C. Silvestri, Susan M. Mccabe, Georg Dorffner, Thomas C. Wetter, Luci Wiggs

Research outputs 2022 to 2026

The Frontiers in Psychiatry Research Theme of Sleep, vigilance, and disruptive behaviors has two aims: first, to promote the understanding of the connections between vigilance and disruptive daytime behavior in the context of sleep deprivation and, second, to explore how naturalistic observations and pattern recognition can play a role in furthering our understanding of these connections. . . .

Measuring Success: Program Fidelity Of Queensland's Child Health Home Visiting Services. A Document Analysis, Nicole Latham, Jeanine Young, Josephine Wilson, Michelle Gray

Research outputs 2022 to 2026

Background: The Family CA.R.E. (Community-based Assistance Resourcing and Education) program was introduced in Queensland two decades ago. It aimed to redress health inequalities for infants from families experiencing specific social stressors. The program has been locally adapted over time and has not been evaluated against the original program. This study assessed the extent to which selected hospital and health services in Queensland, Australia have modified the original Family C.A.R.E. program. Methods: Altheide's model was used to facilitate a critical document analysis of policies and guidelines for adapted Family C.A.R.E. home visiting programs in use by hospital and health services (target …

Midwife Led Randomised Controlled Trials In Australia And New Zealand: A Scoping Review, Caroline Homer, Kim Neylon, Kate Kennedy, Kathleen Baird, Andrea Gilkinson, Samantha Keogh, Sandy Middleton, Richard Gray, Lisa Whitehead, Judith Finn, Claire Rickard, Greg Sharplin, Stephen Neville, Marion Eckert

Research outputs 2022 to 2026

Background: Midwives are the largest workforce involved in caring for pregnant women and their babies, and are well placed to translate research into practice and ensure midwifery priorities are appropriately targeted in researched. Currently, the number and focus of randomised controlled trials led by midwives in Australia and New Zealand is unknown. The Australasian Nursing and Midwifery Clinical Trials Network was established in 2020 to build nursing and midwifery research capacity. To aid this, scoping reviews of the quality and quantity of nurse and midwife led trials were undertaken. Aim: To identify midwife led trials conducted between 2000 and 2021 …

More Than Energy Cost: Multiple Benefits Of The Long Achilles Tendon In Human Walking And Running, Anthony J. Blazevich, Jared R. Fletcher

Research outputs 2022 to 2026

Elastic strain energy that is stored and released from long, distal tendons such as the Achilles during locomotion allows for muscle power amplification as well as for reduction of the locomotor energy cost: as distal tendons perform mechanical work during recoil, plantar flexor muscle fibres can work over smaller length ranges, at slower shortening speeds, and at lower activation levels. Scant evidence exists that long distal tendons evolved in humans (or were retained from our more distant Hominoidea ancestors) primarily to allow high muscle–tendon power outputs, and indeed we remain relatively powerless compared to many other species. Instead, the majority …

Physical Education And Covid-19: What Have We Learned?, Valeria Varea, Ana Riccetti, Gustavo González-Calvo, Marcela Siracusa, Alfonso García-Monge

Research outputs 2022 to 2026

The aim of this paper is to explore what we have learned during the COVID-19 pandemic in the field of Physical Education in three different countries: Argentina, Spain and Sweden. Data were generated through semi-structured interviews, and the concept of field agency is used to make sense of the data. Differences were found among the three countries, regarding the content of the classes, the use of resources, the emotions of teachers, and the use of physical contact. This was also a result of the regulations and resources in place. The COVID-19 pandemic has demonstrated that the nature of the field …

Physiology Of Stretch-Mediated Hypertrophy And Strength Increases: A Narrative Review, Konstantin Warneke, Lars H. Lohmann, Camila D. Lima, Karsten Hollander, Andreas Konrad, Astrid Zech, Masatoshi Nakamura, Klaus Wirth, Michael Keiner, David G. Behm

Research outputs 2022 to 2026

Increasing muscle strength and cross-sectional area is of crucial importance to improve or maintain physical function in musculoskeletal rehabilitation and sports performance. Decreases in muscular performance are experienced in phases of reduced physical activity or immobilization. These decrements highlight the need for alternative, easily accessible training regimens for a sedentary population to improve rehabilitation and injury prevention routines. Commonly, muscle hypertrophy and strength increases are associated with resistance training, typically performed in a training facility. Mechanical tension, which is usually induced with resistance machines and devices, is known to be an important factor that stimulates the underlying signaling pathways to …

Sixteen-Year Longitudinal Evaluation Of Blood-Based Dna Methylation Biomarkers For Early Prediction Of Alzheimer's Disease, Fernanda Schäfer Hackenhaar, Maria Josefsson, Annelie Nordin Adolfsson, Mattias Landfors, Karolina Kauppi, Tenielle Porter, Lidija Milicic, Simon M. Laws, Magnus Hultdin, Rolf Adolfsson, Sofie Degerman, Sara Pudas, Australian Imaging Biomarkers And Lifestyle Study

Research outputs 2022 to 2026

BACKGROUND: DNA methylation (DNAm), an epigenetic mark reflecting both inherited and environmental influences, has shown promise for Alzheimer's disease (AD) prediction. OBJECTIVE: Testing long-term predictive ability ( > 15 years) of existing DNAm-based epigenetic age acceleration (EAA) measures and identifying novel early blood-based DNAm AD-prediction biomarkers. METHODS: EAA measures calculated from Illumina EPIC data from blood were tested with linear mixed-effects models (LMMs) in a longitudinal case-control sample (50 late-onset AD cases; 51 matched controls) with prospective data up to 16 years before clinical onset, and post-onset follow-up. Novel DNAm biomarkers were generated with epigenome-wide LMMs, and Sparse Partial Least Squares …

Contralateral Versus Ipsilateral Protective Effect Against Muscle Damage Of The Elbow Flexors And Knee Extensors Induced By Maximal Eccentric Exercise, Trevor C. Chen, Hsin Lian Chen, Wei Chin Tseng, Tai Ying Chou, Jui Hung Tu, Allen C. Parcell, Kazunori Nosaka

Research outputs 2022 to 2026

The present study compared the ipsilateral repeated bout effect (IL-RBE) and contralateral repeated bout effect (CL-RBE) of the elbow flexors (EF) and knee flexors (KF) for the same interval between bouts to shed light on their mechanisms. Fifty-two healthy sedentary young (20–28 years) men were randomly assigned to the IL-EF, IL-KF, CL-EF, and CL-KF groups (n = 13/group). Thirty maximal eccentric contractions of the EF were performed in IL-EF and CL-EF, and 60 maximal eccentric contractions of the KF were performed in IL-KF and CL-KF, with a 2-week interval between bouts. Changes in muscle damage markers such as maximal voluntary …

Detection Of Metastases Using Circulating Tumour Dna In Uveal Melanoma, Aaron B. Beasley, Daniël P. De Bruyn, Leslie Calapre, Zeyad Al-Ogaili, Timothy W. Isaacs, Jacqueline Bentel, Anna L. Reid, Roy S. Dwarkasing, Michelle R. Pereira, Muhammad A. Khattak, Tarek M. Meniawy, Michael Millward, Erwin Brosens, Annelies De Klein, Fred K. Chen, Emine Kiliҫ, Elin S. Gray

Research outputs 2022 to 2026

Background: Approximately 50% of uveal melanoma (UM) patients will develop metastatic disease depending on the genetic features of the primary tumour. Patients need 3–12 monthly scans, depending on their prognosis, which is costly and often non-specific. Circulating tumour DNA (ctDNA) quantification could serve as a test to detect and monitor patients for early signs of metastasis and therapeutic response. Methods: We assessed ctDNA as a biomarker in three distinct UM cohorts using droplet-digital PCR: (A) a retrospective analysis of primary UM patients to predict metastases; (B) a prospective analysis of UM patients after resolution of their primary tumour for early …