Medicine and Health Sciences Commons^™

Novel Splenic Antigen-Presenting Cells Derive From A Lin-C-Kitlo Progenitor, Pravin Periasamy, Jonathan Tan, Helen O'Neill

Helen O'Neill

No abstract provided.

Cultural Immersion – What Impact Does It Have?, Janie Smith, S Springer, B Murphy, C Wolfe, J Togno, Katrina Bramstedt, Sally Sargeant

Sally Sargeant

No abstract provided.

Antimicrobial Peptide Coating Of Dental Implants: Biocompatibility Assessment Of Recombinant Human Beta Defensin-2 For Human Cells, Patrick Warnke, Eske Voss, Paul Russo, Sebastien Stephens, Michael Kleine, Hendrik Terheyden, Qin Liu

Qin Liu

Purpose: Artificial materials such as dental implants are at risk of bacterial contamination in the oral cavity. Human beta defensins (HBDs), small cationic antimicrobial peptides that exert a broad-spectrum antibacterial function at epithelial surfaces and within some mesenchymal tissues, could probably help to reduce such contamination. HBDs also have protective immunomodulatory effects and have been reported to promote bone remodeling. The aim of this study, therefore, was to investigate the influence of recombinant HBD-2 on the proliferation and survival of cells in culture.

Materials and Methods: Human mesenchymal stem cells (hMSCs), human osteoblasts, human keratinocytes (control), and the HeLa cancer …

Comparison Of In Vitro Biocompatibility Of Nanobone(®) And Biooss(®) For Human Osteoblasts, Qin Liu, Timothy Douglas, Christiane Zamponi, Stephan Becker, Eugene Sherry, Sureshan Sivananthan, Frauke Warnke, Jörg Wiltfang, Patrick Warnke

Qin Liu

Introduction:Scaffolds for bone tissue engineering seeded with the patient's own cells might be used as a preferable method to repair bone defects in the future. With the emerging new technologies of nanostructure design, new synthetic biomaterials are appearing on the market. Such scaffolds must be tested in vitro for their biocompatibility before clinical application. However, the choice between a natural or a synthetic biomaterial might be challenging for the doctor and the patient. In this study, we compared the biocompatibility of a synthetic bone substitute, NanoBone®, to the widely used natural bovine bone replacement material BioOss®.Material and methods: The in …

Nanospiderwebs: Artificial 3d Extracellular Matrix From Nanofibers By Novel Clinical Grade Electrospinning For Stem Cell Delivery, Mohammad Alamein, Qin Liu, Sebastien Stephens, Stuart Skabo, Frauke Warnke, Robert Bourke, Peter Heiner, Patrick Warnke

Qin Liu

Novel clinical grade electrospinning methods could provide three-dimensional (3D) nanostructured biomaterials comprising of synthetic or natural biopolymer nanofibers. Such advanced materials could potentially mimic the natural extracellular matrix (ECM) accurately and may provide superior niche-like spaces on the subcellular scale for optimal stem-cell attachment and individual cell homing in regenerative therapies. The goal of this study was to design several novel “nanofibrous extracellular matrices” (NF-ECMs) with a natural mesh-like 3D architecture through a unique needle-free multi-jet electrospinning method in highly controlled manner to comply with good manufacturing practices (GMP) for the production of advanced healthcare materials for regenerative medicine, and …

Ceramic Scaffolds Produced By Computer-Assisted 3d Printing And Sintering: Characterization And Biocompatibility Investigations, Patrick Warnke, Hermann Seitz, Stephan Becker, Sureshan Sivananthan, Eugene Sherry, Qin Liu, Jorge Wiltfang, Timothy Douglas

Qin Liu

Hydroxyapatite (HAP) and tricalcium phosphate (TCP) are two very common ceramic materials for bone replacement. However, in general HAP and TCP scaffolds are not tailored to the exact dimensions of the defect site and are mainly used as granules or beads. Some scaffolds are available as ordinary blocks, but cannot be customized for individual perfect fit. Using computer-assisted 3D printing, an emerging rapid prototyping technique, individual three-dimensional ceramic scaffolds can be built up from TCP or HAP powder layer by layer with subsequent sintering. These scaffolds have precise dimensions and highly defined and regular internal characteristics such as pore size. …

A Clinically-Feasible Protocol For Using Human Platelet Lysate And Mesenchymal Stem Cells In Regenerative Therapies, Patrick Warnke, Andreas Humpe, Dirk Strunk, Sebastien Stephens, Frauke Warnke, Jorge Wiltfang, Katharina Schallmoser, Mohammad Alamein, Robert Bourke, Peter Heiner, Qin Liu

Qin Liu

The transplantation of human stem cells seeded on biomaterials holds promise for many clinical applications in cranio-maxillo-facial tissue engineering and regenerative medicine. However, stem cell propagation necessary to produce sufficient cell numbers currently utilizes fetal calf serum (FCS) as a growth supplement which may subsequently transmit animal pathogens. Human platelet lysate (HPL) could potentially be utilized to produce clinical-grade stem cell-loaded biomaterials as an appropriate FCS substitute that is in line with clinically-applicable practice. The goal of this study was to investigate whether HPL can be successfully used to propagate human mesenchymal stem cells (HMSCs) seeded on clinically-approved collagen materials …

Mass Production Of Nanofibrous Extracellular Matrix With Controlled 3d Morphology For Large-Scale Soft-Tissue Regeneration, Mohammad Alamein, Sebastien Stephens, Qin Liu, Stuart Skabo, Patrick Warnke

Qin Liu

Aim: Biomaterials that mimic the nanofibrous architecture of the natural extracellular matrix (ECM) are in the focus for stem cell hosting or delivery in tissue engineering of multilayered soft tissues such as skin, mucosa, or retina. Synthetic nanofibers for such ECM are usually produced by single-syringe electrospinning with only one needle-jet at very low production rates of 0.005–0.008 g·min−1. The aim of this study was to utilize a novel industrial needle-free multijet electrospinning device with the potential for mass production of nanofibrous ECM (NF-ECM) exhibiting a controlled three-dimensional (3D) morphology for large-scale applications such as large area skin regeneration in …

The Primordium Of A Biological Joint Replacement: Coupling Of Two Stem Cell Pathways In Biphasic Ultrasound Compressed Gel Niches, Mariea Brady, Sureshan Sivananthan, Vivek Mudera, Qin Liu, Joerg Wiltfang, Patrick Warnke

Qin Liu

The impaired temporomandibular joint might be the first to benefit from applied tissue engineering techniques because it is small and tissue growth in larger amounts is challenging. Bone and cartilage require different competing environmental conditions to be cultivated in vitro. But coupling both the osteogenic and cartilaginous pathways of mesenchymal stem cell differentiation in homeostasis will be a key essential to grow osteochondral constructs or even the first biological joint replacement in the future.

The aim of this study was to test a single source biomaterial and a single source cell type to engineer a biphasic osteochondral construct in vitro …

Novel Ceramic Bone Replacement Material Ceraball® Seeded With Human Mesenchymal Stem Cells, Timothy Douglas, Qin Liu, Andreas Humpe, Jörg Wiltfang, Sureshan Sivananthan, Patrick Warnke

Qin Liu

Objectives: Hydroxyapatite (HA) and tricalcium phosphate (TCP) are two very common ceramic materials for bone replacement. A recently developed material for bone replacement is CeraBall®, which is a mixed HA-TCP scaffold available as porous spherical scaffolds of diameter 4 and 6 mm. Before their use as bone replacement materials in vivo, in vitro testing of these scaffolds is necessary. The goal of this study was to characterise 4 and 6 mm CeraBall® scaffolds in vitro with a view to their future use as bone replacement materials. Materials and methods: The proliferation of human mesenchymal stromal cells (hMSCs) seeded on CeraBall® …

Primordium Of An Artificial Bruch's Membrane Made Of Nanofibers For Engineering Of Retinal Pigment Epithelium Cell Monolayers, Patrick Warnke, Mohammad Alamein, Stuart Skabo, Sebastien Stephens, Robert Bourke, Peter Heiner, Qin Liu

Qin Liu

Transplanted Retinal Pigment Epithelium(RPE) cells hold promise for treatment of Age-related Macular Degeneration(AMD) and Stargardt Disease(SD), but it is conceivable that the degenerated host Bruch's membrane(BM) as a natural substrate for RPE might not optimally support transplanted cell survival with correct cellular organization. We fabricated novel ultrathin 3-dimensional(3D) nanofibrous membranes from Collagen type I and poly(lactic-co-glycolic acid) (PLGA) by an advanced clinical-grade needle-free-electrospinning process. The nanofibrillar 3D networks highly mimicked the fibrillar architecture of the native inner collagenous layer of human BM. Human RPE cells grown on our nanofibrous membranes bore striking resemblance to native human RPE. They exhibited a …

Proliferation Assessment Of Primary Human Mesenchymal Stem Cells On Collagen Membranes For Guided Bone Regeneration, Qin Liu, A. Humpe, D. Kletsas, F. Warnke, S. Becker, T. Douglas, S. Sivananthan, P. Warnke

Qin Liu

Purpose: Human mesenchymal stem cells (hMSCs) hold the potential for bone regeneration because of their self-renewing and multipotent character. The goal of this study was to evaluate the influence of collagen membranes on the proliferation of hMSCs derived from bone marrow. A special focus was set on short-term eluates derived from collagen membranes, as volatile toxic materials washed out from these membranes may influence cell behavior during the short time course of oral surgery.

Materials and Methods: The proliferation of hMSCs seeded directly on a collagen membrane (BioGide) was evaluated quantitatively using the cell proliferation reagent WST-1 (4-3-[4-iodophenyl]-2-[4-nitrophenyl]-2H-[5-tetrazolio]-1, …

Virtual Patient Care: An Interprofessional Education Approach For Physician Assistant, Physical Therapy And Occupational Therapy Students, Michael Shoemaker, Andy Booth, Susan Cleghorn

Michael J Shoemaker, PT, DPT, PhD, GCS

Purpose of Presentation: The purpose of this presentation will be to demonstrate a project in which students from multiple health professional programs were able to work inter-professionally to treat a virtual patient.

Background/Significance: Interprofessional educational experiences are becoming increasingly popular and the evidence for IPE in the training of future health professions is mounting. The importance of interprofessional education (lPE) has gained new ground as a means to improve quality patient care (Remington, 2006). It has been promoted as a method to promote teamwork and increase the ability of health care professionals to learn and work collaboratively (D'Eon, 2005). In …

Disease Dujour: A New Twist On Science Notebooks, Jody Vogelzang

Jody L Vogelzang PhD, RDN, FAND, CHES

Teaching just the facts about genetics, bacteria, viruses, and fungi leave out the practical application of their impact on humans and miss an important teaching opportunity. While a research paper could fill this knowledge gap, it would result in detailed knowledge of just one disease. By using a science notebook as the vehicle for shorter targeted research, students are able to investigate eight different diseases during a nine week grading period. This session will describe the disease notebook assignment, demonstrate how to integrate their new- found knowledge into classroom discussion, show examples of student work, share results of student feedback …

Discharge Checklist To Enhance Rn Role And Prevent Unnecessary Readmissions, Rachel Keene

Rachel Keene, RN, MSN

Discharge is included in every patient's plan of care. It is considered a basic procedure but, without a clear definition of each provider's responsibilities during discharge, duplication of tasks and errors of omission occur (Byrnes et al., 2009; Gawande, 2009). On a medical unit in a Silicon Valley hospital, RNs (Registered Nurse) are often inundated with auxiliary tasks in the hours before discharge. This is partly due to their proximity to the patient, but is mainly from the lack of a standard RN discharge procedure. Such norms jeopardize the RN's ability to provide care and can increase hospital readmissions (Jack …

‘The Dirty War Index: Linking International Humanitarian Law, Public Health And Policy.’, M Hicks

Madelyn Hsiao-Rei Hicks

No abstract provided.

The Evolution Of Retail Clinics In The United States, 2006-2012, Amer Kaissi

Amer A Kaissi

No abstract provided.

The Changing Landscape Of Anesthesia Education: Is Flipped Classroom The Answer?, Denise Hersey, Viji Kurup

Denise Hersey

Risk And Protective Factors Associated With Health-Related Quality Of Life Among Older Gay And Bisexual Men Living With Hiv Disease, Charles A. Emlet, Karen I. Fredriksen-Goldsen

Charles Emlet

No abstract provided.

Endurance And Fatigue Characteristics Of The Neck Muscles In Patients With Cervical Radiculopathy, Marie Halvorsen, Allan Abbott, Anneli Peolsson, Asa Dedering

Allan D. Abbott

Purpose: The aim of the study was to compare myoelectric manifestation in neck muscle endurance and fatigue characteristics during sub-maximal isometric endurance test in patients with cervical radiculopathy and asymptomatic subjects. An additional aim was to explore associations between primary neck muscle endurance, myoelectric fatigability, and self-rated levels of fatigue, pain and subjective health measurements in patients with cervical radiculopathy.

Methods: Muscle fatigue in the ventral and dorsal neck muscles was assessed in patients with cervical radiculopathy and in an asymptomatic group during an isometric neck muscle endurance test in prone and supine. 46 patients and 34 asymptomatic subjects participated. …

Homeotic Gene Teashirt (Tsh) Has A Neuroprotective Function In Amyloid-Beta 42 Mediated Neurodegeneration., Michael M. Moran, Madhuri Kango-Singh, Amit Singh

Amit Singh

Background: Alzheimer's disease (AD) is a debilitating age related progressive neurodegenerative disorder characterized by the loss of cognition, and eventual death of the affected individual. One of the major causes of AD is the accumulation of Amyloid-beta 42 (Aβ42) polypeptides formed by the improper cleavage of amyloid precursor protein (APP) in the brain. These plaques disrupt normal cellular processes through oxidative stress and aberrant signaling resulting in the loss of synaptic activity and death of the neurons. However, the detailed genetic mechanism(s) responsible for this neurodegeneration still remain elusive. Methodology/ Principle Findings: We have generated a transgenic Drosophila eye model …

Introduction To Videos On Gout, Michele Meltzer

Michele Meltzer

No abstract provided.

Cultural Industries And Cultural Policy: A Critique Of Recent Discourses In Regional Economic Development, Chris Gibson, Natascha Klocker

Natascha Klocker

The cultural industries (sometimes referred to as 'creative industries') are an increasingly common component of urban and regional economic development discourse, connected to an acknowledgement of the contribution of creativity to economic performance and, more generally, their power to transform images and identities for places. Such discourses have become more pervasive with a set of key books - most notably Charles Landry's The Creative City (2001), and Florida's The Rise of the Creative Class (2002) - that have become popular among both economic development planners and cultural policy makers. This paper seeks to intervene in the discourses established by these …

The Rural And Remote Health Workforce, G Malone, Janie Smith

Janie Smith

No abstract provided.

Power Distance, Cultural Communication And Medication/Medical Errors: The Avoidance Of An Ethical Dilema, Luanne Linnard-Palmer

Luanne Linnard-Palmer

Academic Publishing As 'Creative' Industry And Recent Discourses Of 'Creative Economies': Some Critical Reflections, Chris Gibson, Natascha Klocker

Natascha Klocker

his paper continues recent discussions on the (geo)politics of the production of academic knowledges, in relation to the recent rise of narratives of 'the creative economy'. Creativity and the 'creative industries' are increasingly common components of urban economic development discourse, especially following the release of a set of key books - most notably Charles Landry's The Creative City (2000), and Richard Florida's The Rise of the Creative Class (2002) - that have become popular among economic development planners and cultural policy makers. This paper focuses on the traffic of these books, and their authors, beyond the Anglo-American core. It also …

The Global Cowboy: Rural Masculinities And Sexualities, Chris Gibson

Chris Gibson

There is arguably no more iconic motif of rural masculinity than the cowboy. The cowboy is a persona, a stereotype, an ideology, and a style of manhood strongly associated with rurality. With origins in Mexico and the American West, cowboy imagery and identities were globalized in the late nineteenth and early twentieth century and were adopted, mutated, and subverted in contexts as different as Hawai'i, urban Japan, and remote Aboriginal Australia. This chapter traces the historical emergence and diffusion of cowboy masculinity, arguing that key to its endurance has been its malleability-its multivalent combinations of hero worship, ambiguity, rural place-based …

Neither Here Nor There Or Always Here And There? Antipodean Reflections On Economic Geography, Felicity Wray, Rae Dufty-Jones, Chris Gibson, Wendy Larner, Andrew Beer, Richard Heron, Phillip O'Neill

Chris Gibson

This paper emerged from discussions held over a two-day symposium hosted by the University of Western Sydney and the Institute of Australian Geographers in December 2011. Drawing on contemporary themes in economic geography around postcolonial theory and a concern with the histories of the sub-discipline, the symposium sought to triangulate these discourses using Raewyn Connell's (2006, 2007a, 2007b) concept of 'Southern Theory' as a means of beginning a process of critical reflection about the types of economic geographies that are produced from and in the 'Antipodes'. After introducing these debates and presenting a critical reflection on how Connell's Southern Theory …

Mild-Mannered Bistro By Day, Eclectic Freak-Land At Night: Memories Of An Australian Music Venue, Ben Gallan, Chris Gibson

Chris Gibson

This article is about a pub that is also a live music venue: the Oxford Tavern in Wollongong. It tells the story of the alternative live music scene that existed there for twenty years before the venue closed in 2010. More than this, it makes an argument for vernacular cultural histories of subcultural places within Australian cities, taking seriously the forgotten venues where marginal social groups find meaning and community. Resonating are more universal themes in Australian cultural life: accommodating difference, a space for expression of otherness, and the importance of music and of a venue in shaping a time …

Urban Cultural Policy, City Size, And Proximity, Chris Gibson, Gordon Waitt

Chris Gibson

In this chapter we bring a distinctly geographical perspective to questions of urban cultural policy. We are interested in how perceptions (and concrete experiences) of city size and proximity shape the politics of urban cultural policymaking. The particular kind of urban cultural policymaking we discuss relates to the pervasive idea that cities ought to refashion their economic development policies and planning regimes to become "creative cities" (Landry 2000). Central to this is an assumption that all places now compete with each other for creative industries and people - the supposed "creative class," who are imagined as a vital demographic group …