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Full-Text Articles in Medicine and Health Sciences
The Multidrug-Resistant Pseudomonas Aeruginosa Clinical Isolates From Dogs And Cats Expressed Three Multidrug Efflux Systems Simultaneously, Kanchana Poonsuk, Rungtip Chuanchuen
The Multidrug-Resistant Pseudomonas Aeruginosa Clinical Isolates From Dogs And Cats Expressed Three Multidrug Efflux Systems Simultaneously, Kanchana Poonsuk, Rungtip Chuanchuen
The Thai Journal of Veterinary Medicine
The objective of this study was to examine the expression of 4 clinically-important Mex systems including MexAB-OprM, MexCD-OprJ, MexEF-OprN and MexXY in the Pseudomonas aeruginosa clinical isolates from dogs and cats. The isolates exhibited high level of resistance to multiple antibiotics clinically important. All of them simultaneously overexpressed up to three different Mex systems, including MexAB-OprM, MexEF-OprN and MexXY as determined by RT-PCR. None of the isolates overexpressed MexCD-OprJ. Expression of mexF was measured by using quantitative real-time RT-PCR. Transcription level of mexF varied (i.e. 4-219 fold) but was at least 4 fold higher than that of PAO1. DNA sequence …
Recombinant Human Follicle Stimulating Hormone And Growth Factors Improve The Meiotic And Developmental Competence Of Cat Oocytes, Theerawat Tharasanit, Chommanart Thongkittidilok, Thanida Sananmuang, Mongkol Techakumphu
Recombinant Human Follicle Stimulating Hormone And Growth Factors Improve The Meiotic And Developmental Competence Of Cat Oocytes, Theerawat Tharasanit, Chommanart Thongkittidilok, Thanida Sananmuang, Mongkol Techakumphu
The Thai Journal of Veterinary Medicine
In vitro embryo production has proven as potential tool for understanding factors involving developmental biology of embryos. This study aimed at examining the effects of recombinant human follicle stimulating hormone (rhFSH) on meiotic resumption and also on developmental competence of cat oocytes. Experiment 1 studied mRNA expression of luteinizing hormone (LH), follicle stimulating hormone (FSH), epidermal growth factor (EGF) and insulin like growth factor-1 (IGF-1) receptors (R) in cumulus cells. mRNA expression of LH receptor in cumulus cells after oocyte maturation was additionally examined. Experiment 2 examined the effect of different rhFSH concentrations (0.01, 0.05, 0.1 and 1 IU/ml) on …