Medicine and Health Sciences Commons^™

Subtotal Adrenalectomy In Multiple Endocrine Neoplasia Type 2, Bertil Hamberger, Margareta Telenius-Berg, Bjorn Cedermark, Staffan Grondal, Bengt-Goran Hansson, Sigbritt Werner

Henry Ford Hospital Medical Journal

We report two patients in whom pheochromocytoma was treated by subtotal adrenalectomy leaving a rim of vascularized cortical tissue in situ. Both patients are doing well without cortisol supplementation although they have subnormal cortisol responses to ACTH stimulation.

Application Of Minisatellite Dna Probes To Linkage In Men-2, Bruce A. J. Ponder, Alec J. Jeffreys, Nicola E. Hartley, Clare Carter, Douglas F. Easton, Hakan Telenius, Margareta Telenius-Berg

Henry Ford Hospital Medical Journal

We describe the potential benefits and the limitations of the use of highly polymorphic minisatellite DNA probes for genetic linkage analysis of multiple endocrine neoplasia type 2A (MEN-2 A). The advantage of these probes is that up to 34 loci can be examined in a single experiment, and since the loci are highly polymorphic, almost every individual in every family is informative. The disadvantage is that the DNA fragment lengths of the alleles at any given locus differ from one family to another, so that families cannot be combined, and large single sibships are needed to obtain significant linkage data. …

One Large Kindred Excludes A Locus For Multiple Endocrine Neoplasia Type 2a From About 25% Of The Human Autosomal Genetic Map, Andrew J. Pakstis, Judith R. Kidd, Carmela M. Castiglione, Beth A. Pletcher, Patricia D. Murphy, Lindsay A. Farrer, Myron Genel, Kenneth K. Kidd

Henry Ford Hospital Medical Journal

This report presents pairwise linkage results from our search for the locus of the gene (MEN2A) for the multiple endocrine neoplasia type 2A (MEN-2A) syndrome in one large kindred (the N kindred), clearly segregating for an autosomal dominant form. About 25% of the autosomal genome is excluded when these new results are combined with those we have published previously. The genetic markers employed are distributed across at least 19 of the 22 autosomes. Seven genetic markers whose chromosomal locations are not yet established have also been studied.

Immunohistochemistry In Medullary Thyroid Carcinoma: Prognosis And Distinction Between Hereditary And Sporadic Tumors, B. Franc, B. Caillou, A. M. Carrier, N. Dutrieux-Berger, J. Floquet, M. Houcke, E. Justrabo, F. Lange, A. Pages, C. Rigaud, A. Schwartz, M. Viennet, M. F. Lebodic

Henry Ford Hospital Medical Journal

In this retrospective study of 68 cases of medullary thyroid carcinoma (MTC), morphological features were studied, which could be helpful in prognosis and in the distinction between hereditary and sporadic forms of the disease. Necrosis within the tumors seemed to carry a poor prognosis. Bilateral MTC. unquestionable C-cell hyperplasia, glandular or follicular pattern, and thyroglobulin-positive cells seemed to be more prevalent in hereditary forms of the disease. A smaller proportion of calcitonin-immunoreactive cells were encountered in hereditary tumors than in sporadic tumors.

Different Mechanisms Of Calcitonin, Calcitonin Gene-Related Peptide, And Somatostatin Regulation By Glucocorticoids In A Cell Culture Of Human Medullary Thyroid Carcinoma, Gilbert J. Cote, Robert F. Gagel

Henry Ford Hospital Medical Journal

We have employed the TT cell line, a model for the human medullary thyroid carcinoma cell, lo study the regulation of peptide hormone production by glucocorticoids. Complementary DNA probes were used to measure the calcitonin (CT), CT gene-related peptide (CGRP), and somatostatin (SRIF) mRNA levels. Dose-response experiments in serum-free medium showed that dexamethasone (six-day treatment) lowered somatostatin (to 1% of basal) and CGRP mRNA (to 50% of basal) and stimulated CT mRNA (threefold to thirteenfold) with a half-maximal effective concentration of 10⁻⁸ M. Time course studies for cells continuously exposed to 10⁻⁶ M dexamethasone showed a rapid (within …

The Aids Project Newsletter (June 1987), David Ketchum, The Aids Project

AIDS Project Newsletter (1987-1991)

No abstract provided.

Front Matter

Henry Ford Hospital Medical Journal

No abstract provided.

The Second International Workshop On Multiple Endocrine Neoplasia Type 2 Syndromes, Bruce A. J. Ponder, Charles E. Jackson

Henry Ford Hospital Medical Journal

No abstract provided.

Screening In Medullary Thyroid Carcinoma, C. Calmettes

Henry Ford Hospital Medical Journal

The four-year experience of the French collaborative study on medullary thyroid carcinoma (MTC) has permitted improvement in family screening by a common discussion of data and a network of coordinators throughout France involved in the detection of new cases. Investigations have been directed at improving the discrimination between sporadic and hereditary forms of MTC and between individuals who have or have not inherited the disease within families.

Men-2 Syndrome: The Value Of Screening And Central Registration; A Study Of Six Kindreds In The Netherlands, H. F. A. Vasen, A. C. Nieuwenhuijzen Kruseman, A. M. J. Moers, C. J. M. Lips, E. K. M. Beukers, W. M. Wiersinga, R. A. Geerdink

Henry Ford Hospital Medical Journal

Since 1975, six families with the MEN-2A syndrome including 66 patients have been identified in The Netherlands. All these patients underwent thyroidectomy for C-cell hyperplasia and/or medullary thyroid carcinoma (MTC); eight were symptomatic (Group A), 51 were relatives of patients found to be affected (Group B), and seven had had a negative screening test that became positive (Group C). To assess the effect of screening, we compared these groups with respect to the occurrence of metastatic MTC at thyroidectomy and the results of the postoperative calcitonin (CT) tests. We found that 87% of Group A, 37% of Group B; and …

Characteristics Of A Family With The Men-2a Syndrome, Harm R. Haak, Arie C. Nieuwenhuijzen Kruseman

Henry Ford Hospital Medical Journal

Results of screening and follow-up in a family with the MEN-2A syndrome are described. Fourteen (83%) subjects at risk were affected with medullary thyroid carcinoma (MTC). Asymptomatic pheochromocytoma and parathyroid hyperplasia were found in two instances each. Intrathyroidal MTC without metastases was found in the four youngest subjects and was associated with normal basal but abnormal pentagastrin-stimulated calcitonin (CT) levels. In the ten subjects with abnormal basal CT levels, metastatic spread to at least regional lymph nodes was found. In eight of these ten subjects, total thyroidectomy with excision of affected lymph nodes and additional radioiodine did not return CT …

Multiple Endocrine Neoplasia Type 2a: A Northern Ireland And Australian Family, David R. Hadden, Fergus O'Reilly, Laurence Kennedy, Colin Russell

Henry Ford Hospital Medical Journal

A Northern Ireland/Australian family with multiple endocrine neoplasia type 2A is described. Three members of the first generation studied have died, two men (aged 35 and 51) as a result of metastatic medullary thyroid carcinoma and one woman (the index case, aged 50) due to hypertensive complications during thyroidectomy from an undiagnosed pheochromocytoma. All members of this family found by screening to have either medullary thyroid carcinoma or pheochromocytoma, or both, have been asymptomatic for their disease.

Screening For Early Asymptomatic Pheochromocytoma In Men-2, Margareta Telenius-Berg, Bertel Berg, Bertil Hamberger, Sten Tibblin

Henry Ford Hospital Medical Journal

Pheochromocytoma is a major cause of morbidity in multiple endocrine neoplasia type 2. We recommend a similar diagnostic screening approach for early asymptomatic pheochromocytoma as is generally accepted for asymptomatic medullary thyroid carcinoma. We present a new provocative test for early asymptomatic pheochromocytoma. Plasma epinephrine levels and epinephrine/dopamine ratios after physical exercise seem to have higher sensitivity than basal plasma catecholamine levels. Specificity is high especially for discrimination from neurovegetative lability, which is the clinically most important differential diagnosis.

Calcitonin Gene-Related Peptide And Calcitonin In Men-2 And Sporadic Pheochromocytomas: An Immunohistochemical Study, Lis Johannsen, Henrik Daa Schroder, Soren Schifter

Henry Ford Hospital Medical Journal

Ten pheochromocytomas (five from patients with multiple endocrine neoplasia type 2 and five sporadic) were stained immunohistochemically with antibodies to calcitonin (CT) and calcitonin gene-related peptide (CGRP) by means of the peroxidase-antiperoxidase method. CGRP positive cells were found in variable numbers in all of the investigated tumors. No demonstrable difference was noted between the hereditary tumors and the sporadic tumors. Staining with CT antibody also showed cells with a positive reaction in eight of ten tumors. The distribution of the two peptides was similar, and in some cells their coexistence was visualized. Pheochromocytomas thus represent an alternative site to medullary …

Calcitonin Gene Peptides: The Diagnostic Value Of Measurement In Medullary Thyroid Carcinoma, Samia I. Girgis, Catherine Lynch, Carmel J. Hillyard, John C. Stevenson, Patricia A. Hill, David W. R. Macdonald, Iain Macintyre

Henry Ford Hospital Medical Journal

The calcitonin gene encodes a family of peptides, at least three of which normally circulate in man: calcitonin (CT), a calcium-lowering hormone; katacalcin (KC), a peptide of unknown function; and calcitonin gene-related peptide (CGRP), a neuropeptide and potent vasodilator. In a study of 45 patients with medullary thyroid carcinoma (MTC), plasma CGRP was elevated in approximately 50% of cases. Furthermore, CGRP levels did not correlate with CT levels. However, plasma KC was elevated in all cases, with a good correlation with CT levels, as has been noted previously. Measurement of CT or KC appears to be superior to measurement of …

Clinical Value Of Calcitonin And Carcinoembryonic Antigen Doubling Times In Medullary Thyroid Carcinoma, Charles E. Jackson, Robert A. Norum, Gary B. Talpos, Carolyn S. Feldkamp, Armen H. Tashjian Jr.

Henry Ford Hospital Medical Journal

Doubling times of basal and stimulated calcitonin (CT) levels and of random carcinoembryonic antigen (CEA) levels were compared over time in 11 patients with residual medullary thyroid carcinoma (MTC)—five with the sporadic MTC and six with the hereditary MTC. Four patients with an indolent form of the disease showed little change in CT levels over three to eight years and little change in CEA doubling times (42 to 70+ months). Seven patients showing rapid progression of disease had CT doubling times of three to 11 months and CEA doubling times of two to 12 months (however, one patient showed no …

Importance Of Ultrasound Examination For The Follow-Up Of Medullary Thyroid Carcinoma: Comparison With Other Localization Methods, Karin Frank, Friedhelm Raue, Dorothea Lorenz, Christian Herfarth, Reinhard Ziegler

Henry Ford Hospital Medical Journal

Ultrasound examinations for the follow-up of 41 patients with histologically proven medullary thyroid carcinoma (MTC) were done regularly at three- to six-month intervals. In patients with elevated serum calcitonin (CT) levels, other localization methods such as computed tomography, selective venous catheterization with measurement of CT levels, and fine-needle aspiration were performed. Thirty-four tumor recurrences in the neck were suspected by ultrasound examination in 23 patients with postoperatively elevated CT levels. The smallest occult MTC nodule had a diameter of 8 mm. One nodule was hyperechogenic with hypoechoic lesions, one was isoechoic with a halo, and all others were hypoechoic. Seventeen …

Radionuclide Imaging In Medullary Thyroid Carcinoma: Evaluation Of Two New Radiopharmaceuticals, S. E. M. Clarke, C. R. Lazarus, M. N. Maisey

Henry Ford Hospital Medical Journal

We assessed the uptake of two new radiopharmaceuticals, 131I meta-iodo-benzylguanidine (MIBG) and 99mTc(V) dimercaptosuccinic acid (DMSA), in patients with histologically proven medullary thyroid carcinoma (MTC). MIBG detected tumor in 40% of patients with known primary or recurrent tumor. 99m Tc(V) DMSA successfully demonstrated primary and recurrent tumor in 86% of patients imaged, with true negative results in 100% of patients imaged after successful surgical resection and a false-negative rate of 14%. We therefore suggest that 99m Tc(V) DMSA is the imaging agent of choice in patients with both primary and recurrent disease. 131I MIBG, with its high false-negative rate, has …

A Two-Site Immunoradiometric Assay For Serum Calcitonin Using Monoclonal Anti-Peptide Antibodies, Philippe Motte, Malika Ait-Abdellah, Pascal Vauzelle, Paule Gardet, Claude Bohuon, Dominique Bellet

Henry Ford Hospital Medical Journal

We have produced a library of monoclonal antibodies of various affinities by immunizing mice with synthetic calcitonin (CT) 1-32. These monoclonal antibodies defined two antigenic determinants on the molecule of CT. The first was located in the 11-17 region of the hormone: the second was present on the 26-32 portion of CT. The C-terminal epitope was restricted to the mature form of the hormone and immunologically silent on synthetic peptides with sequences analogous lo the biosynthetic precursors for CT. Using two high-affinity monoclonal antibodies, designated as CT07 and CT08, we developed a two-site immunoradiometric assay (m-lRMA) for serum CT. This …

In Vitro Secretion Of Peptides Of The Calcitonin Family: Calcitonin, Katacalcin, And Calcitonin Gene-Related Peptide, Friedhelm Raue, Hubert Serve, Eckard Rix, Reinhard Ziegler

Henry Ford Hospital Medical Journal

A tissue culture explant and a primary cell culture of malignant C-cells have been estabUshed to study the secretion pattern of calcitonin (CT), katacalcin (KC), and CT gene-related peptide (CGRP) in vitro under different conditions. Within the first five days of culture the spontaneous secretion of the peptides in both systems dropped to and remained at a constant level. With increasing calcium (Ca) concentration in the medium, a dose dependent release of CT, KC and, in cell culture, of CGRP was observed. BAY-K-8644. an analog of the Ca channel blocker nifedipine, enhanced the CT secretion to the same extent as …

Localization Of A Radiolabeled Monoclonal Antibody To Calcitonin In Rat Medullary Thyroid Carcinoma Allografts, Naguib A. Samaan, Kuo-Pao Yang

Henry Ford Hospital Medical Journal

A radiolabeled anticalcitonin monoclonal antibody (MoAb) was testedfor tumor-localizing capability in WAG/Rij rats allografted with cultured medullary thyroid carcinoma cells (line 44-2). 131I-labeled anticalcitonin IgG was taken up by the tumors in a time-dependent and dose-dependent manner. Tumor tissue uptake of the injected MoAb was 0.7 to 1.3%/g of tumor tissue when tested with a dose range of 40 lo 250 μg/kg of body weight. Distribution ratios of the radiolabeled MoAb were 1.2:1 for tumor-to-blood and 2-20:1 for tumor-to-organs. In rats injected with control IgG, disiribution ratios of radioactivity were 0.4:1 for tumor-to-blood, and 0.6-2:1 for tumor-to-organs. These results suggested …

Studies Of Multiple Endocrine Neoplasia Type 2a Syndrome: Linkage Analyses And Comparison Of Constitutional And Tumor Genotypes, Hideo Tateishi, Shin-Ichiro Takai, Isamu Nishisho, Tetsuro Miki, Kazuyoshi Motomura, Makoto Okazaki, Akira Miyauchi, Tatsuro Ikeuchi, Kohtaro Yamamoto, Tatsuo Hattori, Yuichi Kumahara, Hideo Matsumoto, Tasuko Honjo, Takesada Mori

Henry Ford Hospital Medical Journal

Linkage analyses were carried out in nine Japanese kindreds with multiple endocrine neoplasia type 2A (MEN-2A) using polymorphic classical markers and DNA markers. We excluded close linkage of the MEN-2A gene (MEN2A) locus with Gm, JK, PGMl, and a DNA segment, D20S5, which is assigned to band 12 of the short arm of chromosome 20 (20p12.2). Assuming that MEN2A is recessive at the cellular level as in retinoblastoma (RB) and Wilms' tumor (WT). comparison of constitutional and tumor genotypes may be useful in the search for the MEN2A locus. When DNA samples from 12 patients with medullary thyroid carcinoma (MTC) …

Where Is The Locus For Multiple Endocrine Neoplasia Type 2a?, Nancy E. Simpson, Kenneth K. Kidd

Henry Ford Hospital Medical Journal

Attempts to map the locus of the gene (MEN2A) for the multiple endocrine neoplasia type 2A (MEN-2A) syndrome over the past II years are reviewed. Lod scores from family linkage studies of polymorphisms for 26 proteins and 64 DNA markers (restriction fragment length polymorphisms or RFLPs) were pooled from the published literature and the authors' unpublished data. The pooled data excluded 32% of the genome as possible sites for the MEN2A locus. This represents a considerable increase in the area of exclusion over that of the 6% reported at the first workshop. Linkage studies of RFLPs recognized by DNA sequences …

Back Matter

Henry Ford Hospital Medical Journal

No abstract provided.

The Mexican American Elderly And Barriers To The Utilization Of Health Care Services, Kathleen Anne Hoekstra

Masters Theses

The utilization of health care services by the Mexican American elderly is low compared to the severity of their health problems. This thesis examines the sociocultural factors which affect the interaction of the Mexican American elderly and mainstream health professionals. Included is a brief history detailing discriminatory practices, a description of traditional Mexican cultural practices and attitudes, and an exploration of the cultural barriers which limit access to health care.

Commencement Program 1987, Loma Linda University

Commencement Programs

CONTENTS

2 | 1987 Events of Commencement

3 | Academic Procession

4 | The Speakers

7 | The Honorees

Order of Programs

• School of Dentistry, 12
• School of Medicine, 18
• Graduate School, 24
• School of Nursing, 29
• College of Arts and Sciences, School of Business and Management, and School of Education, 33
• School of Allied Health Professions, 44
• School of Health, 49

Case-Control Study Of Vitamin A Intake And Colorectal Cancer In Seventh-Day Adventists, John William Morgan

Loma Linda University Electronic Theses, Dissertations & Projects

Colorectal cancer is the second most common cause of cancer deaths in Americans; producing approximately 60,000 deaths in Americans each year. Studies of high and low risk populations have suggested a dietary etiology for colorectal cancer, although investigations of specific dietary factors have produced inconsistent results.

Population based studies of California Seventh-day Adventists demonstrate dietary variety regarding factors suspected as important in the etiology of colorectal cancer. These studies document a substantially reduced mortality and incidence from colorectal cancer in Seventh-day Adventists when compared to other California Populations. Although specific reasons for this difference remain obscure, the 'Unique lifestyle and …

Resolving Superfund Disputes Using Mediation, R. Steven Konkel

Environmental Health Science Faculty and Staff Research

Superfund clean-up disputes are difficult to resolve because they involve multiple issues in addition to multiple parties. Environmental Protection Agency (EPA) and waste generator negotiators often disagree about how to interpret technical or scientific information. Examples of difficult issues include company shares of the clean-up cost, potential threat to groundwater, and what environmental standards apply to clean-up; "how clean is clean" is not at all well understood. Industry typically focuses on fairness of the allocation of cost and liability and the cost and responsibility for clean-up.

Osteoporosis And Ert--The Jury Is Still Out, Mary E. Guinan, Karen Steinberg, Lambertina Freni-Titulaer

School of Public Health Faculty Research

Bone loss in women accelerates immediately after menopause, often leading to osteoporosis and an increased risk of bone fractures. In 1985,247,000 hip fractures occurred in people over 45 years of age. Among those who live to be 90, one-third of women and one-sixth of men will experience hip fractures. Six months after fracture, 25% of victims will not be able to carry out their usual activities, and 50% will need assistance to do so. Hip fractures are not only a serious cause of morbidity but they also increase the risk of mortality in older people. Because the proportion of the …

Evaluating The Effect Of Family Stroke Education On The Anxiety Levels Of Families Of Stroke Patients, Keri Lynne Kuniyoshi

Loma Linda University Electronic Theses, Dissertations & Projects

[Abstract Not Included]