Medicine and Health Sciences Commons^™

Alterations In Calcium Homeostasis And The Insulin-Like Growth Factor Signaling Pathway Induced By Carcinogenic Polycyclic And Halogenated Aromatic Hydrocarbons In Human Mammary Epithelial Cells, Stacey L. Tannheimer

Pharmaceutical Sciences ETDs

Breast cancer is a major health concern for women, with only a small percent of the risk factors currently identified. It has been estimated that environmental factors may contribute to up to 80% of breast cancer cases. Many environmental carcinogens, such as polycyclic and halogenated aromatic hydrocarbons (PAHs and HAHs), are proven mammary carcinogens in animal models. Therefore, these studies were conducted to elucidate potential roles of PAHs and HAHs in alterations in known signaling pathways in human mammary epithelial cells (HMEC). Carcinogenic PAHs have previously been shown to produce sustained alterations in the calcium (Ca2') homeostasis of lymphocytes Therefore, …

Interaction Of Gaba And Excitatory Amino Acids In The Basolateral Amygdala: Role In Cardiovascular Regulation, Robert P. Soltis, Jennifer C. Cook, Adam E. Gregg, Brian J. Sanders

Scholarship and Professional Work – COPHS

Activation of the amygdala in rats produces cardiovascular changes that include increases in heart rate and arterial pressure as well as behavioral changes characteristic of emotional arousal. The objective of the present study was to examine the interaction of GABA and excitatory amino acid (EAA) receptors in the basolateral amygdala (BLA) in regulating cardiovascular function. Microinjection of the GABA_A receptor antagonist bicuculline methiodide (BMI) or the E A A receptor agonists NMDA or AMPA into the same region of the BLA of conscious rats produced dose-related increases in heart rate and arterial pressure. Injection of the nonselective EAA receptor …

Nicotinic Receptor Antagonists In The Treatment Of Neuropharmacological Disorders, Peter A. Crooks, Linda P. Dwoskin, Alain Ravard

Pharmaceutical Sciences Faculty Patents

Nicotine analogs that have nicotinic receptor antagonist properties. These compounds have been shown to competitively inhibit dopamine release induced by nicotine. The nicotine analog compounds are useful in the treatment of nicotine abuse, smoking cessation therapy, as an antidote for nicotine intoxication, treatment of cognitive disorders such as Alzheimer's disease and for the treatment of Parkinson's disease.

Chronic Hypothermia And Energy Expenditure In A Neurodevelopmentally Disabled Patient: A Case Study, Jane M. Gervasio, Roland N. Dickerson, Rex O. Brown, J. Barret Matthews

Scholarship and Professional Work – COPHS

Hypothermia is defined as a core body temperature of <35°C and results in a decrease in measured resting energy expenditure. A 51-year-old mentally disabled patient experienced chronic hypothermia from neurologic sequelae. Because of her continued weight gain and increased body fat in the presence of presumed hypocaloric nutrition, indirect calorimetry measurements were performed twice in a 3-month period. The resting energy expenditure measurements prompted a reduction of her daily caloric intake to prevent further overfeeding. Hypothermia reduces oxygen consumption and, as a consequence, decreases resting energy expenditure. In patients for whom chronic hypothermia is a problem, nutritional intake must be adjusted to prevent overfeeding, excessive weight gain, and the long-term complications of an excess of total calories.

Multicompartment Hard Capsule With Control Release Properties, George Digenis, Dagmar Noskova

Pharmaceutical Sciences Faculty Patents

The present invention relates to a hard capsule made from a material such as gelatin, starch or a hydrophilic polymer. The capsule of the present invention is formulated into a delivery system which incorporates pharmacologically active components in three or more distinct compartments. When three compartments are utilized in the capsule, the outer compartment incorporates a drug and excipients into a layer which coats the outer part of the capsule. This layer represents the rapid release portion of the delivery system. The intermediate compartment comprises a powder formulation comprised of a drug and excipients which represents the intermediary speed release …

Enteral Cisapride, Erythromycin, And Metoclopramide In Critically Ill Patients Intolerant To Enteral Nutrition: A Randomized, Placebocontrolled, Cross-Over Study, Robert Maclaren, Jane M. Gervasio, David A. Kuhl, Teresa N. Livingston, Kyle Swift, Roland N. Dickerson, Rex O. Brown, Stacey Headley, Kenneth A. Kudsk, John J. Lima

Scholarship and Professional Work – COPHS

Abstract from American College of Clinical Pharmacy 1997 Annual Meeting, Phoenix, AZ, November 9-12, 1997.

Measured Energy Expenditure Of Nonambulatory Patients With Severe Neurodevelopmental Disabilities, Roland N. Dickerson, Rex O. Brown, Jane M. Gervasio, Emily B. Hak, John E. Williams, Lawrence J. Hak

Scholarship and Professional Work – COPHS

Abstract from American College of Clinical Pharmacy 1997 Annual Meeting, Phoenix, AZ, November 9-12, 1997.

Aminoalkylpyridine Compounds Which Are Useful As Anticonvulsant Drugs, Excitatory Amino Acid Inhibitors And Nmda Sigma Receptor Antagonists, Pankaja K. Kadaba

Pharmaceutical Sciences Faculty Patents

Pharmaceutical compositions comprise as the active ingredient potent orally active, nonneurotoxic anticonvulsant compounds that are excitatory amino acid and NMDA/sigma receptor antagonists . . .

To read the remainder of this abstract, please download this patent.

Summer 1997, Southwestern Oklahoma State University

The Sig

The Sig Newsletter from the Southwestern Oklahoma State University College of Pharmacy - Summer 1997

Pharmacology Of Antiparkinsonian Agents, Robert P. Soltis

Scholarship and Professional Work – COPHS

The following is a summary of a two hour class on the basic pharmacology of antiparkinsonian agents. It is presented to fourth-year pharmacy students in pharmacotherapeutics III, a course structured using team-taught modules. Faculty from the Department of Pharmacy Sciences provide instruction on the basic pharmacology of therapeutic agents and faculty from the Department of Pharmacy Practice follow up with a discussion of the therapeutic applications of these agents. This course is lecture-based with opportunities for in-class discussion. One week prior to the lecture sequence on the basic pharmacology of antiparkinsonian drugs, students are provided a handout that includes the …

Alterations In Human B Cell Calcium Homeostasis By Polycyclic Aromatic Hydrocarbons: Possible Associations With Cytochrome P450 Metabolism And Increased Tyrosine Phosphorylation, Barbara J. Mounho

Pharmaceutical Sciences ETDs

Polycyclic aromatic hydrocarbons (PAHs), are known immunotoxicants in animals, and are suspect toxins to the human immune system. The mechanism(s), however, by which PAHs exert immunosuppression have not been fully elucidated. Previous studies conducted in our laboratory have shown that PAHs, such as 7,12-dimethylbenz(a)- anthracene (DMB A) and benzo(a)pyrene (BaP) may exert their immunotoxic effects by altering intracellular calcium (Ca2+) homeostasis in lymphocytes. Intracellular Ca2+ is an important second messenger in the immune response, and the mobilization of Ca2+ is critical in the transduction of intracellular signals from the plasma membrane to the nucleus. The overall objective of this project …

Enhancing Compliance With Allergic Rhinitis Therapy, Beth Wofford, Deborah S. Zeitlin

Scholarship and Professional Work – COPHS

Allergic rhinitis is one of the most common chronic conditions in the US, surpassing even heart disease. Over $1 billion is spent annually for over-the-counter (OTC) and prescription medications. In addition, allergic rhinitis causes an estimated annual loss of $639 million related to lost work days and diminished work performance.

If not effectively treated, allergic rhinitis may lead to potential complications such as otitis media, sinusitis, facial and dental abnormalities, hearing loss, poor speech development, and recurrent upper respiratory tract infections. Ineffective treatment often the result of intentional or unintentional noncompliance and inadequate patient education. Therefore, it is imperative that …

Health Professions Division Catalog Academic Year 1997-1998, Nova Southeastern University

Health Professions Divisions Course Catalogs and Course Descriptions

No abstract provided.

Multiple Ionic Conductances Of The Human Dopamine Transporter: The Actions Of Dopamine And Psychostimulants, Mark S. Sonders, Si-Jia Zhu, Nancy R. Zahniser, Michael Kavanaugh, Susan G. Amara

Biomedical and Pharmaceutical Sciences Faculty Publications

Electrophysiological and pharmacological studies of a cloned human dopamine transporter (hDAT) were undertaken to investigate the mechanisms of transporter function and the actions of drugs at this target. Using two-electrode voltage-clamp techniques with hDAT-expressing Xenopus laevis oocytes, we show that hDAT can be considered electrogenic by two criteria. (1) Uptake of hDAT substrates gives rise to a pharmacologically appropriate “transport-associated” current. (2) The velocity of DA uptake measured in oocytes clamped at various membrane potentials was voltage-dependent, increasing with hyperpolarization. Concurrent measurement of transport-associated current and substrate flux in individual oocytes revealed that charge movement during substrate translocation was greater …

Excitatory Amino Acid Transporter 5, A Retinal Glutamate Transporter Coupled To A Chloride Conductance, Jeffrey L. Arriza, Scott Eliasof, Michael Kavanaugh, Susan G. Amara

Biomedical and Pharmaceutical Sciences Faculty Publications

Although a glutamate-gated chloride conductance with the properties of a sodium-dependent glutamate transporter has been described in vertebrate retinal photoreceptors and bipolar cells, the molecular species underlying this conductance has not yet been identified. We now report the cloning and functional characterization of a human excitatory amino acid transporter, EAAT5, expressed primarily in retina. Although EAAT5 shares the structural homologies of the EAAT gene family, one novel feature of the EAAT5 sequence is a carboxy-terminal motif identified previously in N-methyl-d-aspartate receptors and potassium channels and shown to confer interactions with a family of synaptic proteins that promote ion channel …

Aspartate 19 And Glutamate 121 Are Critical For Transport Function Of The Myo-Inositol/H+ Symporter From Leishmania Donovani, Andreas Seyfang, Michael Kavanaugh, Scott M. Landfear

Biomedical and Pharmaceutical Sciences Faculty Publications

The protozoan flagellate Leishmania donovani has an active myo-inositol/proton symporter (MIT), which is driven by a proton gradient across the parasite membrane. We have used site-directed mutagenesis in combination with functional expression of transporter mutants in Xenopusoocytes and overexpression in Leishmania transfectants to investigate the significance of acidic transmembrane residues for proton relay and inositol transport. MIT has only three charged amino acids within predicted transmembrane domains. Two of these residues, Asp¹⁹ (TM1) and Glu¹²¹ (TM4), appeared to be critical for transport function of MIT, with a reduction of inositol transport to about 2% of wild-type …

Tyrosine 140 Of The Γ-Aminobutyric Acid Transporter Gat-1 Plays A Critical Role In Neurotransmitter Recognition, Yona Bismuth, Michael Kavanaugh, Baruch I. Kanner

Biomedical and Pharmaceutical Sciences Faculty Publications

The γ-aminobutyric acid (GABA) transporter GAT-1 is located in nerve terminals and catalyzes the electrogenic reuptake of the neurotransmitter with two sodium ions and one chloride. We now identify a single tyrosine residue that is critical for GABA recognition and transport. It is completely conserved throughout the superfamily, and even substitution to the other aromatic amino acids, phenylalanine (Y140F) and tryptophan (Y140W), results in completely inactive transporters. Electrophysiological characterization reveals that both mutant transporters exhibit the sodium-dependent transient currents associated with sodium binding as well as the chloride-dependent lithium leak currents characteristic of GAT-1. On the other hand, in both …

Mutation Of An Amino Acid Residue Influencing Potassium Coupling In The Glutamate Transporter Glt-1 Induces Obligate Exchange, Michael Kavanaugh, Annie Bendahan, Noa Zerangue, Yumin Zhang, Baruch I. Kanner

Biomedical and Pharmaceutical Sciences Faculty Publications

Glutamate transporters maintain low synaptic concentrations of neurotransmitter by coupling uptake to flux of other ions. After cotransport of glutamic acid with Na⁺, the cycle is completed by countertransport of K⁺. We have identified an amino acid residue (glutamate 404) influencing ion coupling in a domain of the transporter implicated previously in kainate binding. Mutation of this residue to aspartate (E404D) prevents both forward and reverse transport induced by K⁺. Sodium-dependent transmitter exchange and a transporter-mediated chloride conductance are unaffected by the mutation, indicating that this residue selectively influences potassium flux coupling. The results …

Book Review Of Baruch A. Brody, Ethical Issues In Drug Testing, Approval, And Pricing, Annalee Abelson

RISK: Health, Safety & Environment (1990-2002)

Review of Baruch A. Brody, Ethical Issues in Drug Testing, Approval, and Pricing (Oxford University Press 1995). Conclusions, index, introduction, preface. LC 94-4479; ISBN 0-19-508831-X. [268 pp. Cloth $35.00. 200 Madison Avenue, New York NY 10016.]

1997 Apothecary, Southwestern Oklahoma State University

Apothecary

The 1997 Apothecary Staff congratulates the graduating seniors! We know the education received at Southwestern Oklahoma State University School of Pharmacy will provide many opportunities to become a valuable asset to the pharmacy profession. Best wishes for a successful career.

Apparatus And Method For Noninvasive Chemical Analysis, Robert A. Lodder

Pharmaceutical Sciences Faculty Patents

A method and apparatus are provided for magnetohydrodynamic acoustic-resonance, near-IR spectroscopy. The method includes a step of applying to a subject under study a magnetic field having a strength between 2,00-10,000 gauss, near-IR radiation having a wavelength between 800-3,000 nm and an acoustic wave having a frequency between 10 khz -1 Mhz. The method also includes the steps of inducing vibration of ions in the magnetic field and detecting an electric wave generated magnetohydrodynamically by the acoustic wave induced vibration of the ions. Next is the collecting of the electrical, acoustical and near-IR spectra and the analyzing of the collected …

Use Of L-Canavanine As A Chemotherapeutic Agent For The Treatment Of Pancreatic Cancer, Peter Crooks, Gerald A. Rosenthal

Pharmaceutical Sciences Faculty Patents

A pharmaceutical composition of canavanine, and a method treatment of cancer, particularly pancreatic cancer with L-canavanine is disclosed.

Low Molecular Weight Thiocarbamates As Inhibitors Of Elastase, Uses And Method Of Synthesis, George Digenis, Nikolaos P. Rodis

Pharmaceutical Sciences Faculty Patents

To see this abstract, please download this patent.

Anion Induced Blue To Purple Transition In Bacteriorhodopsin, Mrunalini Pattarkine, Anil K. Singh

Faculty Works

Purple membrane (PM, λ" role="presentation">λmax" role="presentation">max 570 nm) of H. halobium on treatment with sulphuric acid changes its colour to blue (λ" role="presentation">λmax" role="presentation">max 608 nm). The purple chromophore can be regenerated from the blue chromophore by exogeneous addition of anions such as CI−" role="presentation">− and HPO42−" role="presentation">2−4. Chloride ion is found to be more effective than the dibasic phosphate ion in regenerating the purple chromophore. Nevertheless, one thing common to the anion regeneration is that both CI−" role="presentation">− and HPO42−" role="presentation">2−4 show marked pH effect. At pH 1.0 the efficiency of …

Spring 1996, Southwestern Oklahoma State University

The Sig

The Sig Newsletter from the Southwestern Oklahoma State University College of Pharmacy - Spring 1996

Coated-Products With Potent Anti-Hiv And Antimicrobial Properties, George Digenis, Alexander G. Digenis

Pharmaceutical Sciences Faculty Patents

A composition which has potent anti-HIV activity. The composition is a high energy coprecipitate of nonoxynol-9 oligomers, polyvinyl-pyrrolidone (PVP) and iodine or PVP-I and shows a pronounced synergistic, anti-HIV effect between the compounds of the composition. The invention also includes paper and plastic products coated with the composition of the invention.

Pharmacokinetics Of Vancomycin In Critically Ill Infants Undergoing Extracorporeal Membrane Oxygenation, R. Davis Maker, Joseph T. Dipiro, Jatinder Bhatia

Publications from the Office of the Dean

Extracorporeal membrane oxygenation (ECMO) is a widely used therapy for neonates with respiratory failure. Because of sepsis, many of these infants require antibiotics like vancomycin during ECMO treatment. ECMO transiently alters renal function and increases the circulating blood volume by 75%. Initial vancomycin pharmacokinetics were determined in 12 infants undergoing ECMO to determine an adequate drug administration regimen. Vancomycin dosage was based on current recommendations for weight and gestational age. Pharmacokinetic parameters were determined by fitting the data to a two compartment model. This study yielded a mean steady-state volume of distribution of 1.1 +/- 0.5 (range, 0.6 to 2.1) …

Asct-1 Is A Neutral Amino Acid Exchanger With Chloride Channel Activity, Noa Zerangue, Michael Kavanaugh

Biomedical and Pharmaceutical Sciences Faculty Publications

The ubiquitous transport activity known as system ASC is characterized by a preference for small neutral amino acids including alanine, serine, and cysteine. ASCT-1 and ASCT-2, recently cloned transporters exhibiting system ASC-like selectivity, are members of a major amino acid transporter family that includes a number of glutamate transporters. Here we show that ASCT1 functions as an electroneutral exchanger that mediates negligible net amino acid flux. The electrical currents previously shown to be associated with ASCT1-mediated transport result from activation of a thermodynamically uncoupled chloride conductance with permeation properties similar to those described for the glutamate transporter subfamily. Like glutamate …

Kinetics And Stoichiometry Of A Proton/Myo-Inositol Cotransporter, Elizabeth M. Klamo, Mark E. Drew, Scott M. Landfear, Michael Kavanaugh

Biomedical and Pharmaceutical Sciences Faculty Publications

Voltage clamp recording was used to measure steady-state and presteady-state currents mediated by a myo-inositol transporter cloned from Leishmania donovani and expressed in Xenopus oocytes. Application of myo-inositol resulted in inward currents, which did not require external sodium and which were increased by increasing the extracellular proton concentration and by membrane hyperpolarization. Alkalinization of the extracellular space occurred concomitantly with myo-inositol influx. Correlation of membrane currents with radiolabeled myo-inositol flux revealed that one positive charge is translocated with each molecule of myo-inositol, consistent with cotransport of one proton. The transport concentration dependence on both species …

1996 Apothecary, Southwestern Oklahoma State University

Apothecary

The 1996 Apothecary staff would like to congratulate the graduating seniors on their successful completion of the professional coursework here at Southwestern Oklahoma State University School of Pharmacy. We trust that their gained knowledge and education will allow them to obtain professional success and, through all their future endeavors, further advance the pharmacy profession.