Medicine and Health Sciences Commons^™

Detection Of Forces And Body Load In Standing And Walking In The American Cockroach, J. Adam Noah

Theses, Dissertations and Capstones

Sense organs in the legs that detect body weight are an important component in the regulation of posture and locomotion. This thesis seeks to gain an understanding of how body weight is detected by sense organs of the legs, and determine how this information influences muscle activities in standing and walking. The first study tested the ability of tibial campaniform sensilla (receptors that monitor forces in the cockroach leg) to encode variations in body load using magnets attached to the thorax. Recordings of sensory activities in freely standing animals showed that proximal tibial sensilla (oriented perpendicular to the leg long …

The Neural Connectivity Of The Torus Semicircularis In The Cave Dwelling Form Of Astyanax Fasciatus, Timothy B. Angel

Theses, Dissertations and Capstones

Afferent projections of the torus semicircularis were traced in the blind cave form of Astyanax fasciatus using HRP tract-tracing techniques, and the results were compared to those found in previous studies of closely related sighted teleosts.

Following unilateral injections of tracer to the nucleus centralis of the torus semicircularis, retrogradely labeled cells were found in two octaval nuclei of the medulla. The descending octavolateralis nucleus was labeled bilaterally and the anterior octaval nucleus was labeled primarily on the ipsilateral side. Both the descending and anterior octavolateralis nuclei are known to receive auditory input from CN VIII in sighted fish. In …

The Characterization Of Two Differentially Expressed Plasma Proteins In Obese Versus Lean Rats In Two Rodent Models Of Obesity, Roger D. Boggs

Theses, Dissertations and Capstones

Zucker fa and La/N faf rats are widely studied models of genetic obesity and its complications. These two rodent models of obesity were utilized to search for a circulating protein marker for obesity. Plasma samples from both of these models of obesity were collected and analyzed via SDS-PAGE analysis. Two proteins were found which demonstrate differential expression between lean and obese rats. Both proteins demonstrated increased expression in the obese rats compared to the lean. One differentially expressed protein migrated on SDS-PAGE gels at 116 KD while the second migrated at 22 KD compared to molecular weight markers. The 22 …

A Mechanistic Study In The Nephrotoxicity Of P-Aminophenol, R. Christopher Harmon

Theses, Dissertations and Capstones

The acetaminophen metabolite, p-aminophenol (PAP), is a known nephrotoxicant. The current study is directed at understanding the mechanism of this nephrotoxicity. Renal cortical slices isolated from F344 rats were incubated with 0 – 0.5 mM PAP with or without pretreatments. Renal slices exposed to PAP showed toxicity as measured by increased lactate dehydrogenase leakage (LDH), decreased pyruvate-stimulated gluconeogenesis, and decreased total adenine nucleotides. There was, however, an increase in ATP as percent of total, implying maintained mitochondrial function. These data show that PAP is directly toxic to renal cortical slices. Pretreatment with EDTA or EGTA provided protection from PAP- induced …

Adaptive Multimodal Biometric Fusion Algorithm Using Particle Swarm, Kalyan Veeramachaneni, Lisa Ann Osadciw, Pramod K. Varshney

Electrical Engineering and Computer Science - All Scholarship

This paper introduces a new algorithm called “Adaptive Multimodal Biometric Fusion Algorithm”(AMBF), which is a combination of Bayesian decision fusion and particle swarm optimization. A Bayesian framework is implemented to fuse decisions received from multiple biometric sensors. The system’s accuracy improves for a subset of decision fusion rules. The optimal rule is a function of the error cost and a priori probability of an intruder. This Bayesian framework formalizes the design of a system that can adaptively increase or reduce the security level. Particle swarm optimization searches the decision and sensor operating points (i.e. thresholds) space to achieve the desired …

Equilibrative Nucleoside Transporter Family Members From Leishmania Donovani Are Electrogenic Proton Symporters, Alex Stein, Gayatri Vaseduvan, Nicola S. Carter, Buddy Ullman, Scott M. Landfear, Michael Kavanaugh

Biomedical and Pharmaceutical Sciences Faculty Publications

Leishmania donovani express two members of the equilibrative nucleoside transporter family; LdNT1 encoded by two closely related and linked genes, LdNT1.1 and LdNT1.2, that transport adenosine and pyrimidine nucleosides and LdNT2 that transports inosine and guanosine exclusively. LdNT1.1, LdNT1.2, and LdNT2 have been expressed in Xenopus laevis oocytes and found to be electrogenic in the presence of nucleoside ligands for which they mediate transport. Further analysis revealed that ligand uptake and transport currents through LdNT1-type transporters are proton-dependent. In addition to the flux of protons that is coupled to the transport reaction, LdNT1 transporters mediate a variable constitutive proton …

Health Professions Division Catalog 2003-2004, Nova Southeastern University

Health Professions Divisions Course Catalogs and Course Descriptions

No abstract provided.

The Ca2+ Dynamics Of Isolated Mouse Β-Cells And Islets: Implications For Mathematical Models, Min Zhang, Paula Goforth, Richard Bertram, Arthur Sherman, Leslie S. Satin

Pharmacology and Toxicology Publications

ABSTRACT

[Ca²⁺]_i and electrical activity were compared in isolated β-cells and islets using standard techniques. In islets, raising glucose caused a decrease in [Ca²⁺]_i followed by a plateau and then fast (2–3 min⁻¹), slow (0.2–0.8 min⁻¹), or a mixture of fast and slow [Ca²⁺]_i oscillations. In β-cells, glucose transiently decreased and then increased [Ca²⁺]_i, but no islet-like oscillations occurred. Simultaneous recordings of [Ca²⁺]_i and electrical activity suggested that differences in [Ca²⁺]_i signaling are due to …

Relationships Between Human Auditory Cortical Structure And Function, Deborah A Hall, Heledd C Hart, Ingrid Johnsrude

Brain and Mind Institute Researchers' Publications

The human auditory cortex comprises multiple areas, largely distributed across the supratemporal plane, but the precise number and configuration of auditory areas and their functional significance have not yet been clearly established. In this paper, we discuss recent research concerning architectonic and functional organisation within the human auditory cortex, as well as architectonic and neurophysiological studies in non-human species, which can provide a broad conceptual framework for interpreting functional specialisation in humans. We review the pattern in human auditory cortex of the functional responses to various acoustic cues, such as frequency, pitch, sound level, temporal variation, motion and spatial location, …

Analysis Of Gene × Environment Interactions In Sibships Using Mixed Models, Jill S. Barnholtz-Sloan, Laila M. Poisson, Steven W. Coon, Gary A. Chase, Benjamin A. Rybicki

Wayne State University Associated BioMed Central Scholarship

Abstract

Background

Gene × environment models are widely used to assess genetic and environmental risks and their association with a phenotype of interest for many complex diseases. Mixed generalized linear models were used to assess gene × environment interactions with respect to systolic blood pressure on sibships adjusting for repeated measures and hierarchical nesting structures. A data set containing 410 sibships from the Framingham Heart Study offspring cohort (part of the Genetic Analysis Workshop 13 data) was used for all analyses. Three mixed gene × environment models, all adjusting for repeated measurement and varying levels of nesting, were compared for …

The Role Of Ap-1 Transcription Complex In Retinoic Acid-Dependent B16 Melanoma Cell Growth Arrest And Differentiation, Ying Huang

Theses, Dissertations and Capstones

Our laboratory has been studying the effects of all-trans retinoic acid (atRA) on the growth and differentiation of the B16 mouse melanoma cells. RA inhibits both anchorage-dependent and –independent growth and stimulates melanin production in B16 cells, which are accompanied by an increase in AP-1 activity. To further investigate the RA-induced AP-1 activity, I established clones of B16 stably expressing an AP-1-luciferase reporter gene. Treatment of these clones with phorbol dibutyrate (PDB), a known activator of AP-1, rapidly increased AP-1 activity which reached its maximum level at 2-4 h and returned to baseline level by 24 h. In contrast, RA …

Zo1 As A Factor In The Remodeling Of Myocardial Gap Junctions, Ralph John Barker

MUSC Theses and Dissertations

The remodeling of connexin43-containing gap junctions (GJs), as defined by changes in size, distribution, or function, is a prominent feature of both the developing and diseased myocardium. During postnatal development of the mammalian ventricle, there is a progressive remodeling of GJs into intercalated disks. This change in GJ quantity and distribution is correlated with alterations in the velocity and anisotropy of impulse spread within differentiating myocardium. Orderly distribution of GJs is perturbed in many cardiac diseases, and reemergence of immature patterns of myocyte coupling may be a factor in the arrhythmogenic potential attending such pathologies. Although mechanisms responsible for this …

Structural Analyses Of Phycodnaviridae And Iridoviridae, Alan A. Simpson, Narayanasamy Nandhagopal, James L. Van Etten, Michael G. Rossmann

Nebraska Center for Virology: Faculty Publications

The Phycodnaviridae, Iridoviridae and related viruses, with diameters of 1500±2000 A Ê , are formed from large trigonal arrays of hexagonally close-packed capsomers forming the faces of icosahedra [Yan et al. (2000), Nature Struct. Biol. 7, 101-103; Nandhagopal et al. (2002), Proc. Natl Acad. Sci. USA, 99, 14758-14763]. Caspar and Klug predicted that such structures could be assembled from hexameric capsomers [Caspar & Klug (1962), Cold Spring Harbor. Symp. Quant. Biol. 27, 1-24], as was subsequently found in numerous icosahedral viruses. During the course of evolution, some viruses, including the virus families …

Development Of An Automated Thiopurine Methyltransferase (Tpmt) Genotyping Assay, Mary Corcoran

Theses

Thiopurine methyltransferase (TPMT) is a cytosolic enzyme that catalyses the S- methylation of aromatic and sulphydryl compounds known as the thiopurines. These therapeutic agents are used in the treatment of acute lymphoblastic leukemia, autoimmune disorders, inflammatory bowel disease and organ transplant recipients.

Erythrocyte TPMT activity is polymorphic and patients with intermediate or deficient TPMT are at significant risk for excessive toxicity after receiving ‘standard’ doses of thiopurine medications. The activity of TPMT is inherited as an autosomal co-dominant trait. Several mutations in the TPMT gene have been described that correlate with a low activity phenotype. Three mutations G238C, G460A and …

Isovolumetric Regulation In Mammal Cells: Role Of Taurine, B. Ordaz, R. Franco, National University Of Mexico Tuz

School of Veterinary and Biomedical Sciences: Faculty Publications

The ability to regulate cell volume is an ancient conserved trait present in essentially all species through evolution. The maintenance of a constant cell volume is a homeostatic imperative in animal cells. Changes in cell water content affecting the concentration of intracellular messenger molecules impair the complex signaling network, crucial for cell functioning and intercellular communication. Although the renal homeostatic mechanisms exert a precise control of extracellular fluid osmolarity, this is challenged in a variety of pathological situations. The intracellular volume constancy is continuously compromised by the generation of local and transient osmotic microgradients, associated with nutrients uptake, secretion, cytoskeleton …

The Role Of Oxidative Stress In Alzheimer's Disease, Robin Petroze

Kaleidoscope

Over four million individuals in the United States currently suffer from Alzheimer's disease (AD), a devastating disorder of progressive dementia. Within the next several decades, AD is expected to affect over 22 million people globally. AD can only be definitively diagnosed by postmortem examination. Thus, investigation into the specific pathogenesis of neuronal degeneration and death in AD on a biochemical level is essential for both earlier diagnosis and potential treatment and prevention options. Overproduction of amyloid [3-peptide (A[3) in the brain leads to both free radical oxidative stress and toxicity to neurons in AD. My undergraduate biochemical studies with regard …

The Relationship Between Thigh Muscle Size And 1rm Squat Strength Among Bodybuilders, Powerlifters, And Olympic Weightlifters, James Dinaso

Masters Theses

The purpose of this study was to determine if a significant difference exists in the relationship between measures of muscle size and strength among elite bodybuilders, powerlifters, and Olympic weightlifters. Fifteen male subjects between the ages of 16 and 48 years participated in the study. All three subject groups, Olympic weightlifters (OWL, n=5), powerlifters (PL, n=5), and bodybuilders (BB, n=5), were highly trained and currently involved in competition training. All test subjects were of similar body weight and weighed between 76-96 kilograms. Measures of body weight, body composition (bioelectrical impedence), shoulder width, thigh circumference (proximal, distal, and mid-thigh), and thigh …

Environmental Health In Public Health, Steven Konkel

Books/Book chapters

No abstract provided.

Functional Implications In Apoptosis By Interferon Inducible Gene Product 1-8d, The Binding Protein To Adenovirus Preterminal Protein, Insil Joung, Peter C. Angeletti, Jeffrey A. Engler

Nebraska Center for Virology: Faculty Publications

Adenovirus (Ad) precursor to the terminal protein (pTP) plays an essential roles in the viral DNA

replication. Ad pTP serves as a primer for the synthesis of a new DNA strand during the initiation

step of replication. In addition, Ad pTP forms organized spherical replication foci on the nuclear

matrix (NM) and anchors the viral genome to the NM. Here we identified the interferon inducible

gene product 1-8D (Inid) as a pTP binding protein by using a two-hybrid screen of a HeLa cDNA

library. Of the clones obtained in this assay, nine were identical to the Inid, a 13-kDa polypeptide …

Table Of Contents

Journal of the South Carolina Academy of Science

No abstract provided.

President's Message

Journal of the South Carolina Academy of Science

No abstract provided.

Histological Effect Of Ascocotyle Tenuicollis (Digenea: Heterophyidae) Metacercarial Infection On The Heart Of Fundulus Heteroclitus (Teleostei: Cyprinodontidae), Toni Hicks, Edna Steele

Journal of the South Carolina Academy of Science

No abstract provided.

Par Mediation Of Thrombin-Induced Effects On Motoneurons, Nadim Salman, Drew Watkins, Kate Hamel, Lakisha Gadsden, Sarah Funderburk, Victoria Turgeon

Journal of the South Carolina Academy of Science

No abstract provided.

Embryogenesis In The Japanese Medaka, Oryzias Latipes, Laura Harris Harris, Lisa Sims, Roxann Stachiw

Journal of the South Carolina Academy of Science

No abstract provided.

2002 & 2003 Annual Meeting Extended Abstracts

Journal of the South Carolina Academy of Science

No abstract provided.

South Carolina Statewide Science Safety Project., Linda Sinclair, Jack Gerlovich, Rahul A. Parsa

Journal of the South Carolina Academy of Science

No abstract provided.

Some Notes On Multiplicative Congruential Random Number Generators With Mersenne Prime Modulus 261-1., James Harris

Journal of the South Carolina Academy of Science

No abstract provided.

Maternal And Sibling Influences On Seed Germination Of Aegilops Triuncialis Erin, Erin Scott, Andrew R. Dyer

Journal of the South Carolina Academy of Science

No abstract provided.

Scas Officers And Council Members 2003 - 2004

Journal of the South Carolina Academy of Science

No abstract provided.

Critical Thinking And Biology Instruction., David J. Stroup, John L. Frola

Journal of the South Carolina Academy of Science

No abstract provided.