Medicine and Health Sciences Commons^™

Novel Molecular Markers Of Malignancy In Histologically Normal And Benign Breast, Aejaz Nasir, Dung-Tsa Chen, Mike Gruidl, Evita B. Henderson-Jackson, Chinnambally Venkataramu, Susan M. Mccarthy, Heyoung L. Mcbrid, Eleanor Harris, Nazanin Khakpour, Timothy J. Yeatman

Pathology and Cell Biology Faculty Publications

To detect the molecular changes of malignancy in histologically normal breast (HNB) tissues, we recently developed a novel 117-gene-malignancy-signature. Here we report validation of our leading malignancy-risk-genes, topoisomerase-2-alpha (TOP2A), minichromosome-maintenance-protein-2 (MCM2) and “budding-uninhibited-by-benzimidazoles-1-homolog-beta” (BUB1B) at the protein level. Using our 117-gene malignancy-signature, we classified 18 fresh-frozen HNB tissues from 18 adult female breast cancer patients into HNB-tissues with low-grade (HNB-LGMA; ) and high-grade molecular abnormality (HNB-HGMA; ). Archival sections of additional HNB tissues from these patients, and invasive ductal carcinoma (IDC) tissues from six other patients were immunostained for these biomarkers. TOP2A/MCM2 expression was assessed as staining index (%) and …

Ovarian Epithelial-Stromal Interactions: Role Of Interleukins 1 And 6, Kamisha T. Woolery, Patricia A. Kruk

Pathology and Cell Biology Faculty Publications

Ovarian epithelial cancer is the most lethal gynecologic malignancy. The high mortality is attributed to the fact that most cases typically present in late stage when ovarian cancer (OC) has already spread beyond the ovary. Ovarian epithelial cancer cells are shed into intraperitoneal ascites and easily disseminate throughout the peritoneal cavity with preferential metastasis to the omentum, peritoneum, and local organs. Understanding how ovarian epithelial cells interact with and modulate their microenvironment can provide insight into the molecular mechanism(s) involved with malignant transformation and progression which may eventually identify novel diagnostic, prognostic, and therapeutic targets. The objective of this paper …

The Evolution Of Host Specialization In The Vertebrate Gut Symbiont Lactobacillus Reuteri, Steven A. Frese, Andrew K. Benson, Gerald W. Tannock, Diane M. Loach, Jaehyoung Kim, Min Zhang, Phaik Lyn Oh, Nicholas C. K. Heng, Prabhu B. Patil, Nathalie Juge, Donald A. Mackenzie, Bruce M. Pearson, Alla Lapidus, Eileen Dalin, Hope Tice, Eugene Goltsman, Miriam Land, Loren Hauser, Natalia Ivanova, Nikos C. Kyrpides, Jens Walter

Jens Walter Publications

Recent research has provided mechanistic insight into the important contributions of the gut microbiota to vertebrate biology, but questions remain about the evolutionary processes that have shaped this symbiosis. In the present study, we showed in experiments with gnotobiotic mice that the evolution of Lactobacillus reuteri with rodents resulted in the emergence of host specialization. To identify genomic events marking adaptations to the murine host, we compared the genome of the rodent isolate L. reuteri 100-23 with that of the human isolate L. reuteri F275, and we identified hundreds of genes that were specific to each strain. In …

Effects Of Xs® Energy Drink On Aerobic Exercise Capacity Of Athletes, Kevin M. Sheehan, Lynn K. Hartzler

Biological Sciences Faculty Publications

A small increase in performance is often the difference between winning and losing for athletes, and energy supplements are marketed to give them a competitive edge. One such supplement is XS® Energy Drink which contains B vitamins, caffeine, taurine, and an herbal adaptogen blend. XS® Energy Drink is primarily designed and marketed as an alertness stimulus; however, it is also marketed to athletes as a performance enhancing supplement. The hypothesis of this investigation was that the consumption of XS® Energy Drink before exercise would increase aerobic capacity (an increase in Vo2max) and time to fatigue, and would decrease time for …

The Mechanosensory Role Of Primary Cilia In Vascular Hypertension, Surya M. Nauli, Xingjian Jin, Beerend P. Hierck

Pharmacy Faculty Articles and Research

Local regulation of vascular tone plays an important role in cardiovascular control of blood pressure. Aside from chemical or hormonal regulations, this local homeostasis is highly regulated by fluid-shear stress. It was previously unclear how vascular endothelial cells were able to sense fluid-shear stress. The cellular functions of mechanosensory cilia within vascular system have emerged recently. In particular, hypertension is insidious and remains a continuous problem that evolves during the course of polycystic kidney disease (PKD). The basic and clinical perspectives on primary cilia are discussed with regard to the pathogenesis of hypertension in PKD.

Inhibition Of Multi-Drug Resistant Hiv-1 Reverse Transcriptase By Nucleoside Beta-Triphosphates, Chandravanu Dash, Yousef Ahmadibeni, Michael J. Hanley, Jui Pandhare, Mathias Gotte, Stuart F. J. Le Grice, Keykavous Parang

Pharmacy Faculty Articles and Research

Despite the success of potent reverse transcriptase (RT) inhibitors against human immunodeficiency virus type 1 (HIV-1) in combination regimens, the development of drug resistant RTs constitutes a major hurdle for the long-term efficacy of current antiretroviral therapy. Nucleoside β-triphosphate analogs of adenosine and nucleoside reverse transcriptase inhibitors (NRTIs) (3′-azido-2′,3′-dideoxythymidine (AZT), 3′-fluoro-2′,3′-dideoxythymidine (FLT), and 2′,3′-didehydro-2′,3′-dideoxythymidine (d4T)) were synthesized and their inhibitory activities were evaluated against wild-type and multidrug resistant HIV-1 RTs. Adenosine β-triphosphate (1) and AZT β-triphosphate (2) completely inhibited the DNA polymerase activity of wild type, the NRTI multi resistant, and nonnucleoside RT inhibitors (NNRTI) resistant HIV-1 RT at 10 …

Thiazolyl N-Benzyl-Substituted Acetamide Derivatives: Synthesis, Src Kinase Inhibitory And Anticancer Activities, Asal Fallah-Tafti, Alireza Foroumadi, Rakesh Tiwari, Amir Nasrolahi Shirazi, David G. Hangauer, Yahao Bu, Tahmineh Akbarzadeh, Keykavous Parang, Abbas Shafiee

Pharmacy Faculty Articles and Research

KX2-391 (KX-01/Kinex Pharmaceuticals), N-benzyl-2-(5-(4-(2-morpholinoethoxy)phenyl)pyridin-2-yl)acetamide, is a highly selective Src substrate binding site inhibitor. To understand better the role of pyridine ring and N-benzylsubstitution in KX2-391 and establish the structure-activity relationship, a number of N-benzyl substituted (2-morpholinoethoxy)phenyl)thiazol-4-yl)acetamide derivatives containing thiazole instead of pyridine were synthesized and evaluated for Src kinase inhibitory activities. The unsubstituted N-benzyl derivative (8a) showed the inhibition of c-Src kinase with GI50 values of 1.34 μM and 2.30 M in NIH3T3/c-Src527F and SYF/c-Src527F cells, respectively. All the synthesized compounds were evaluated for inhibition of cell proliferation of human colon carcinoma (HT-29), breast carcinoma (BT-20), and leukemia (CCRF-CEM) cells. …

Altered Self-Assembly And Apatite Binding Of Amelogenin Induced By N-Terminal Proline Mutation, Li Zhu, Vuk Uskoković, Thuan Le, Pamela Denbesten, Yulei Huang, Stefan Habelitz, Wu Li

Pharmacy Faculty Articles and Research

Objective—A single Pro-70 to Thr (p.P70T) mutation of amelogenin is known to result in hypomineralized amelogenesis imperfecta (AI). This study aims to test the hypothesis that the given mutation affects the self-assembly of amelogenin molecules and impairs their ability to conduct the growth of apatite crystals.

Design—Recombinant human full-length wild-type (rh174) and p.P70T mutated amelogenins were analyzed using dynamic light scattering (DLS), protein quantification assay and atomic force microscopy (AFM) before and after the binding of amelogenins to hydroxyapatite crystals. The crystal growth modulated by both amelogenins in a dynamic titration system was observed using AFM.

Results—As …

Polyethylenimine-Conjugated Gold Nanoparticles: Gene Transfer Potential And Low Toxicity In The Cornea, Ajay Sharma, Ashish Tandon, Jonathan C. K. Tovey, Rangan Gupta, J. David Robertson, Jennifer A. Fortune, Alexander M. Klibanov, John W. Cowden, Frank G. Rieger, Rajiv R. Mohan

Pharmacy Faculty Articles and Research

This study examined the gene transfer efficiency and toxicity of 2-kDa polyethylenimine conjugated to gold nanoparticles (PEI2-GNP) in the human cornea in vitro and rabbit cornea in vivo. PEI2-GNP with nitrogen-to-phosphorus (N/P) ratios of up to 180 exhibited significant transgene delivery in the human cornea without altering the viability or phenotype of these cells. Similarly, PEI2-GNP applied to corneal tissues collected after 12 h, 72 h, or 7 days exhibited appreciable gold uptake throughout the rabbit stroma with gradual clearance of GNP over time. Transmission electron microscopy detected GNP in the keratocytes and the extracellular matrix of the rabbit corneas. …

Targeted Identification Of Metastasis-Associated Cell-Surface Sialoglycoproteins In Prostate Cancer, Lifang Yang, Julius O. Nyalwidhe, Sigi Guo, Richard R. Drake, O. John Semmes

Bioelectrics Publications

Covalent attachment of carbohydrates to proteins is one of the most common post-translational modifications. At the cell surface, sugar moieties of glycoproteins contribute to molecular recognition events involved in cancer metastasis. We have combined glycan metabolic labeling with mass spectrometry analysis to identify and characterize metastasis-associated cell surface sialoglycoproteins. Our model system used syngeneic prostate cancer cell lines derived from PC3 (N2, nonmetastatic, and ML2, highly metastatic). The metabolic incorporation of AC₄ManNAz and subsequent specific labeling of cell surface sialylation was confirmed by flow cytometry and confocal microscopy. Affinity isolation of the modified sialic-acid containing cell surface proteins …

Roles Of The 15-Kda Selenoprotein (Sep15) In Redox Homeostasis And Cataract Development Revealed By The Analysis Of Sep 15 Knockout Mice, Marina V. Kasaikina, Dmitri E. Fomenko, Vyacheslav M. Labunskyy, Salil A. Lachke, Wenya Qiu, Juliet A. Moncaster, Jie Zhang, Mark W. Wojnarowicz, Sathish Kumar Natarajan, Mikalai I. Malinouski, Ulrich Schweizer, Petra A. Tsuji, Bradley A. Carlson, Richard L. Maas, Marjorie F. Lou, Lee E. Goldstein, Dolph L. Hatfield, Vadim N. Gladyshev

School of Veterinary and Biomedical Sciences: Faculty Publications

The 15-kDa selenoprotein (Sep15) is a thioredoxin-like, endoplasmic reticulum-resident protein involved in the quality control of glycoprotein folding through its interaction with UDP-glucose:glycoprotein glucosyltransferase. Expression of Sep15 is regulated by dietary selenium and the unfolded protein response, but its specific function is not known. In this study, we developed and characterized Sep15 KO mice by targeted removal of exon 2 of the Sep15 gene coding for the cysteinerich UDP-glucose:glycoprotein glucosyltransferase-binding domain. These KO mice synthesized a mutant mRNA, but the shortened protein product could be detected neither in tissues nor in Sep15 KO embryonic fibroblasts. Sep15 KO mice were viable …

A Computational Framework For Proteome-Wide Pursuit And Prediction Of Metalloproteins Using Icp-Ms And Ms/Ms Data, W. Andrew Lancaster, Jeremy L. Praissman, Farris L. Poole, Aleksandar Cvetkovic, Angeli Lal Menon, Joseph W. Scott, Francis E. Jenney, Michael P. Thorgersen, Ewa Kalisiak, Junefredo V. Apon, Sunia A. Trauger, Gary Siuzdak, John A. Tainer, Michael W. W. Adams

PCOM Scholarly Works

BACKGROUND: Metal-containing proteins comprise a diverse and sizable category within the proteomes of organisms, ranging from proteins that use metals to catalyze reactions to proteins in which metals play key structural roles. Unfortunately, reliably predicting that a protein will contain a specific metal from its amino acid sequence is not currently possible. We recently developed a generally-applicable experimental technique for finding metalloproteins on a genome-wide scale. Applying this metal-directed protein purification approach (ICP-MS and MS/MS based) to the prototypical microbe Pyrococcus furiosus conclusively demonstrated the extent and diversity of the uncharacterized portion of microbial metalloproteomes since a majority of the …

Analysis Of Gaba And Glutamate In The Mouse Dorsal Striatum, Stella Wisidagamage

Wayne State University Theses

ABSTRACT

ANALYSIS OF GABA AND GLUTAMATE IN MICE BRAIN

by

STELLA WISIDAGAMA

May 2011

Advisor: Dr. Tiffany Mathews

Major: Chemistry (Analytical)

Degree: Master of Science

GABA and glutamate are the predominant inhibitory and excitatory amino acid neurotransmitters in the central nervous system (CNS), respectively. However, their altered levels cause several neurological diseases including Parkinson's and Huntington's disease, schizophrenia, and epilepsy. It is important to measure their levels in the extra- and intracellular environments to better understand and develop improved therapeutics that will treat these neurological disorders. The principle aim of this study is to study the impacts of different endogenous …

Significant Inhibition Of Corneal Scarring In Vivo With Tissue-Selective, Targeted Aav5 Decorin Gene Therapy, Rajiv R. Mohan, Ashish Tandon, Ajay Sharma, John W. Cowden, Jonathan C. K. Tovey

Pharmacy Faculty Articles and Research

PURPOSE. This study tested a hypothesis that tissue-selective targeted decorin gene therapy delivered to the stroma with adeno-associated virus serotype 5 (AAV5) inhibits corneal fibrosis in vivo without significant side effects.

METHODS. An in vivo rabbit model of corneal fibrosis was used. Targeted decorin gene therapy was delivered to the rabbit cornea by a single topical application of AAV5 (100 L; 6.5 1012 g/mL) onto the bare stroma for 2 minutes. The levels of corneal fibrosis were determined with stereomicroscopy, slit lamp biomicroscopy, -smooth muscle actin ( SMA), fibronectin, and F-actin immunocytochemistry, and/or immunoblotting. CD11b, F4/80 immunocytochemistry, and TUNEL assay …

Spinal Cord Stimulator Induced Artifact During A Sleep Study, Robert J. Corba Do

Department of Anesthesiology

No abstract provided.

Pediatrician Perceptions Of The Patient-Centered Medical Home Model, Christopher Ray

Theses and Dissertations

The Patient-Centered Medical Home (PCMH) is an emerging model of health care designed to provide a simpler, more effective health care experience. The model places heavy emphasis on the concept of every patient having a "personal physician" who is the point of access for all health care needs and concerns. The personal physician integrates all relevant health care information to provide the patient with a holistic picture of his health. The supposed benefits of the PCMH model include an improved patient experience, increased effectiveness of care, increased efficiency of care, greater access to care, among others. Only now is evidence …

Evaluation Of The Relationship Between Clinical Parameters Of Mineral And Bone Disease, Fibroblast Growth Factor 23, And Related Medication Use Among Hemodialysis Patients, Magdalene Marie Assimon

Legacy Theses & Dissertations (2009 - 2024)

Background

Hypolipidemic And Antioxidant Activity Of Aqueous Extract Of Monascus Purpureus Fermented Indian Rice In High Cholesterol Diet Fed Rats, Aiyalu Rajasekaran, Muthusamy Kalaivani

Turkish Journal of Medical Sciences

To evaluate hypolipidemic and anti-oxidant activities of Monascus purpureus fermented Indian variety of rice. Materials and methods: Indian rice variety IR-532-E-576 was fermented with Monascus purpureus MTCC 1090 (Monascaceae) and extracted with water by boiling. Experimentally induced hyperlipidemia was produced by feeding rats with 2% cholesterol enriched diet for 15 days. Afterwards hypercholesterolemic rats were orally administered with Monascus fermented Indian rice extract at the dose of 1.2 and 2.4 mg/kg bw along with high cholesterol diet for 30 successive days in order to evaluate their expected hypolipidemic activity. Plasma lipid parameters such as total cholesterol, triglycerides, LDL (low density …

Plasma Adiponectin And Resistin Levels In Women With Polycystic Ovary Syndrome: Relation To Body Mass Index And Insulin Resistance, Emre Di̇kmen, İlhan Tarkun, Funda Öztürk, Berri̇n Arslan, Zeynep Cantürk

Turkish Journal of Medical Sciences

To compare the serum levels of adiponectin and resistin between patients with polycystic ovary syndrome (PCOS) and healthy control subjects matched for age and body mass index (BMI), and to assess possible correlations of adiponectin and resistin to the hormonal and metabolic parameters of the syndrome. Materials and methods: Fifty five patients with PCOS diagnosis composed of 25 obese, 13 overweight, 17 normal weight subjects, and 49 healthy women matched for age and BMI were included in this study. Hormone and lipid profile, adiponectin, and resistin were measured in all cases. Results: Serum adiponectin levels were similar in the lean …

Atypical Acute Urticaria In Children And Its Relationship With Urticarial Vasculitis, Zafer Arslan, Serap Özmen, Sara Sürmeli̇, Ni̇lüfer Arda

Turkish Journal of Medical Sciences

In childhood, urticarial lesions are sometimes associated with purpura. This form might be identified as atypical, and may also be related to urticarial vasculitis (UV). The aim of this study was to assess the clinicopathologic characteristics of UV in children with atypical urticaria. Materials and methods: Fifteen children with atypical urticaria were evaluated with medical history, physical examination, and laboratory and skin punch biopsy findings. Results: Infections were detected as possible precipitating factor in 12 patients. Complement levels were normal in all. On histological examination, 6 patients (40%) had neutrophil-predominant infiltrate and 9 (60%) patients had lymphocyte-predominant infiltrate. All the …

Comparison Of Growth Factor Levels In Patients With Normal And Hypospadiac Prepuce*, Tutku Soyer, Ebru Şebnem Ayva, Pinar Atasoy, Mustafa Kemal Aslan, Ahmet Murat Çakmak

Turkish Journal of Medical Sciences

To compare the growth factor levels in patients with normal and hypospadiac prepuce. Materials and methods: Patients who underwent hypospadias repair were included in the study. The prepuce was excised after achieving successful hypospadias repair with satisfactory functional and cosmetic appearance. The patients who underwent preputial reconstructions and complicated repairs were excluded. The control group consists of boys without hypospadias. Elective circumcision was performed to have normal preputial samples. The VEGF levels, VEGF receptor (VEGFR) and TGF-\beta receptor (TGF-\beta-R) expressions were evaluated in preputial tissues by immunohistochemical analysis. Results: Fifteen patients were included in each group. The mean age of …

Efficacy Of Hyaluronic Acid - Carboxymethyl Cellulose Membrane (Seprafilm®) And Polylactic Acid Barrier Film (Surgiwrap™) For The Prevention Of Adhesions After Thyroid Surgery: An Experimental Model, Gökhan Çi̇pe, Hakan Mustafa Köksal, Sadik Yildirim, Mustafa Fevzi̇ Celayi̇r, Adi̇l Baykan

Turkish Journal of Medical Sciences

Adhesions occurring after initial surgery increase complications in secondary thyroid operations. Therefore use of anti-adhesive barriers may reduce complication rates of secondary thyroid operations. In this study, we investigated the efficacy of hyaluronic acid-carboxymethyl cellulose membrane (HA-CMC, Seprafilm®) and polylactic acid barrier film (PLA, Surgiwrap™) in reducing adhesions after thyroid surgery. Materials and methods: A randomized-controlled experimental animal model was utilized. Forty-eight male Wistar Albino rats were randomly divided into 3 equal groups, forming study and control groups. Thyroid surgery was performed in all subjects. HA-CMC and PLA were applied between the thyroid zone and strap muscles after thyroid surgery …

Management Of Community-Acquired Acute Bacterial Cystitis In Turkey, Ömer Coşkun, Hakan Erdem, Ali̇ Avci

Turkish Journal of Medical Sciences

Community acquired acute bacterial cystitis (CA-ABC) is one of the most common infectious presentations in the community. This paper aims to provide a rational approach in the management of CA-ABC based on local Turkish data. Materials and methods: The publications evaluating the microbiological data obtained from CA-ABC cases were searched in both Turkish and international databases. Results: Fosfomycin and nitrofurantain appear to be baseline antibiotics while the empirical use of trimethoprim sulfamethoxazole, amoxicillin clavulanate, and first and second generation cephalosporins seems to be unreliable in Turkey. Moreover, quinolones seem to be at the edge with resistance rates up to 20%. …

The Short Term Effect Of The Law Prohibiting Smoking In Enclosed Areas Upon The Smoking Conditions Of Employees In Kayseri, Turkey, Ahmet Öztürk, Serpi̇l Poyrazoğlu, Şule Şarli

Turkish Journal of Medical Sciences

A law prohibiting smoking in the enclosed areas of public and private workplaces was put into effect in Turkey on 19 May 2008. The objective of this study was to evaluate the effect of this law upon the smoking habits of the employees in this short period of 3 months. Materials and methods: This study was performed in Kayseri, in 3 public and 3 private workplaces, comprising 868 employees. A questionnaire was filled out, face-to-face, in the second week of August. Results: The ratio of the people supporting this smoke-free law was 68.7%. Among the employees who were smoking when …

Are Gram Negative Bacilli A Threat For Prosthetic Joint Replacements? Experience Of A University Hospital, Emi̇ne Alp Meşe, Mehmet Halici, Emi̇ne Bi̇lge Kiran, Mahmut Argün, Mehmet Doğanay

Turkish Journal of Medical Sciences

No abstract provided.

Ex Vivo Produced Oral Mucosa Equivalent Preliminary Report: A Technical Note, Gürkan Raşi̇t Bayar

Turkish Journal of Medical Sciences

The aim of this study was to produce of ex vivo oral mucosal equivalent. The continuous stratified layer of human oral keratinocytes was grown on a cadaveric human dermal matrix in a defined culture medium without a feeder layer. Materials and methods: Human oral keratinocytes from a keratinized oral mucosa biopsy specimen were enzymatically dissociated and cultured in a serum-free defined culture medium, "EpiLife" (Cascade Biologics, Portland, OR, USA), containing a low calcium concentration of 0.06 mM. Oral keratinocytes were expanded one passage. Once a sufficient population of keratinocytes was reached, they were seeded onto the type IV collagen coated …

Evaluation Of Rubella, Toxoplasma Gondii, And Cytomegalovirus Seroprevalences Among Pregnant Women In Denizli Province, Aysun Karabulut, Yusuf Polat, Meral Türk, Yasemi̇n Işik Balci

Turkish Journal of Medical Sciences

There is no national screening program for rubella, Toxoplasma gondii, and cytomegalovirus (CMV) infections for pregnant women in Turkey. The purpose of this study was to determine the prevalence of these infections among pregnant women in Denizli province, and to review seroprevalences reported from different regions of Turkey. Materials and methods: Between April 2008 and April 2009, 1268 women were investigated for rubella, 1102 for toxoplasma, and 1000 women for CMV seroprevalences in the first trimester of pregnancy. Toxoplasma and rubella IgM and IgG antibodies were assayed by the automated Vitros ECiQ system based on an immunometric technique. Cytomegalovirus IgM …

A Comparison Of The Effects Of Intrathecal Ropivacaine And Bupivacaine During Cesarean Section*, Nuray Camgöz Eryilmaz, Berri̇n Günaydin

Turkish Journal of Medical Sciences

To compare the effects of intrathecal plain bupivacaine or ropivacaine with those of opioids on sensory and motor block characteristics during cesarean section (C/S). Materials and methods: Fifty-two ASA I or II women were randomly allocated into 2 groups to administer either 10 mg of 0.5% plain bupivacaine (Group B), or 15 mg of 0.75% plain ropivacaine (Group R) with 25 \mug fentanyl and 100 µg morphine for spinal anesthesia. Characteristics of the sensory and motor block were recorded. Results: The time to achieve sensory block at T_6 was significantly faster in Group B than in Group R (2.7 ± …

In Vitro Effects Of Some Drugs On Human Erythrocyte Glutathione Reductase, Ebru Akkemi̇k, Murat Şentürk, Fatma Betül Özgeri̇ş, Pinar Taşer, Mehmet Çi̇ftci̇

Turkish Journal of Medical Sciences

The effects of dacarbazine, thiocolchicoside, methotrexate, olanzapine, pantoprazole sodium, and 5-fluorouracil on the enzyme activity of glutathione reductase (GR) were studied using human erythrocyte GR enzymes in in vitro studies, respectively. Materials and methods: The enzyme was purified 3333-fold from human erythrocytes in a yield of 44.44% with 4.0 U/mg. The purification procedure involved the preparation of hemolysate, ammonium sulfate precipitation, 2', 5'-ADP Sepharose 4B affinity chromatography, and Sephadex G-200 gel filtration chromatography. SDS-PAGE showed a single band for enzyme. Purified enzyme was used in the in vitro studies. Results: In the in vitro studies, IC50 values and Ki constants …

Suicide Attempts With Amitriptyline In Adults: A Prospective, Demographic, Clinical Study, Şahi̇n Aslan, Mücahi̇t Emet, Zeynep Çakir, Ayhan Aköz, Sultan Tuna Akgöl Gür

Turkish Journal of Medical Sciences

There are several studies on tricyclic antidepressant (TCA) intoxication in the literature; however, there is no specific study on amitriptyline, a specific TCA, intoxication. Materials and methods: Forty adult patients who had been admitted to the emergency department due to suicide attempts with amitriptyline were included. Medical and social histories of the patients were obtained. The estimated amount of amitriptyline ingested was determined. The psychiatric support and psychiatric diagnosis were all recorded. All patients were contacted by phone 6 months later. Results: Main reason of suicidal attempt was family problems. The stated amount of ingested amitriptyline by the patient or …