Medicine and Health Sciences Commons^™

Quantitation Of Ortho-Cresyl Phosphate Adducts To Butyrylcholinesterase In Human Serum By Immunomagnetic-Uhplc-Ms/Ms, Darryl Johnson, Melissa D. Carter, Brian S. Crow, Samantha L. Isenberg, Leigh Ann Graham, H. Akin Erol, Caroline M. Watson, Brooke G. Pantazides, Marcel J. Van Der Schans, Jan P. Langenberg, Daan Noort, Thomas A. Blake, Jerry D. Thomas, Rudolph C. Johnson

United States Public Health Resources

Tri-ortho-cresyl phosphate (ToCP) is an anti-wear, flame retardant additive used in industrial lubricants, hydraulic fluids and gasoline. The neurotoxic effects of ToCP arise fromthe liver-activated metabolite 2-(o-cresyl)-4H-1,3,2-benzodioxaphosphoran-2-one (cresyl saligenin phosphate or CBDP),which inhibits esterase enzymes including butyrylcholinesterase (BChE). Following BChE adduction, CBDP undergoes hydrolysis to form the aged adduct ortho-cresyl phosphoserine (oCP-BChE), thus providing a biomarker of CBDP exposure. Previous studies have identified ToCP in aircraft cabin and cockpit air, but assessing human exposure has been hampered by the lack of a laboratory assay to confirm exposure. This work presents the development of an immunomagnetic- UHPLC-MS/MS method for the quantitation …

Characterization Of Stard4 And Stard6 Proteins In Human Ovarian Tissue And Human Granulosa Cells And Cloning Of Human Stard4 Transcripts, Aisha Shaaban

Theses and Dissertations

Progesterone is essential hormone for pregnancy, which is produced by the human corpus luteum in early pregnancy until the placenta assumes this function. Transport of the cholesterol from the outer to the inner mitochondrial membrane is the rate limiting step for the de novo synthesis of pregnenolone (the precursor to progesterone), a process mediated by STARD1. STARD1 contains a C-terminal lipid binding domain which binds cholesterol and an N-terminal domain targeting STARD1 to the mitochondrial membrane. Unlike STARD1, STARD4 and STARD6 proteins lack a mitochondrial targeting sequence; however, they can bind cholesterol and increase steroidogenesis in model systems. In this …

Neural Correlates Of Episodic Memory Formation In Children And Adults, Lingfei Tang

Wayne State University Theses

The medial temporal lobe (MTL) and prefrontal cortex (PFC) are two key brain regions that support episodic memory formation in both children and adults, but the functional developmental of these regions remains unclear. In this study, we investigated the development of neural correlates of episodic memory formation using functional MRI with a subsequent memory paradigm, administered to a cross-sectional sample of 83 children and adults. We found that MTL subregions showed an age-related increase in activation supporting memory formation of complex scenes. In addition, a functionally defined scene-sensitive region in the posterior MTL also showed similar increase and predicted better …

Using The Symmetry Of False Matches To Solve The Correspondence Problem, Cherlyn J. Ng, Bart Farell

Biomedical and Chemical Engineering - All Scholarship

Veridical stereoscopic depth depends on matching corresponding image points. This requires solving the stereo correspondence problem: how are true matches distinguished from false ones? Conventional algorithms select true matches on the basis of feature detection [what do you mean by ‘feature detection’ here? Is there some more specific term?] and adherence to natural statistics. They reject false matches as noise. We propose here an alternative that uses the signals present in false matches to delineate the true solution. When visualized in a Keplerian array, binocular matches are symmetrically reflected about an axis that is a potential solution. Properties such as …

The Effects Of Curcumin On The Expression Of P53 And Bcl-2 Oncoproteins On The Human Breast Carcinoma Cell Line Mda-Mb 468, Angelin S. Shajan

Theses and Dissertations

We studied the effects of curcumin on the proliferation and viability of MDA-MB-468 breast cancer cells and its effects on the expression ofBcl-2 and p53 proteins in these cells. Based on previous studies, we predicted a dose-dependent increase in p53 (tumor suppressor) expression, and a decrease in Bcl-2 (anti-apoptotic oncoprotein) expression. Briefly, MDA-MB468 cells were seeded into 12-well plates at 2.0 * 104 cells/well and allowed to grow for 72 hrs. Cells were treated with curcumin at 0, 25, 50 and 100 pM in triplicate at time zero and cultured for another 24 hours. All data were obtained using a …

Autism Awareness: Debunking Misconceptions, Nicole Summers

Honors Projects

No abstract provided.

Design, Synthesis, Antiviral Activity, And Pre-Formulation Development Of Poly-Larginine- Fatty Acyl Derivatives Of Nucleoside Reverse Transcriptase Inhibitors, Bhanu P. Pemmaraju, Swapnil Malekar, Hitesh K. Agarwal, Rakesh Tiwari, Donghoon Oh, Gustavo F. Doncel, David R. Worthen, Keykavous Parang

Pharmacy Faculty Articles and Research

The objective of this work was to design conjugates of anti-HIV nucleosides conjugated with fatty acids and cell-penetrating poly-L-arginine (polyArg) peptides. Three conjugates of polyArg cell-penetrating peptides with fatty acyl derivatives of alovudine (FLT), lamivudine (3TC), and emtricitabine (FTC) were synthesized. In general, the compounds exhibited anti-HIV activity against X4 and R5 cell-free virus with EC50 values of 1.5–16.6 μM. FLT-CO-(CH2)12-CO-(Arg)7 exhibited EC50 values of 2.9 μM and 3.1 μM against X4 and R5 cell-free virus, respectively. The FLT conjugate was selected for further preformulation studies by determination of solution state degradation and lipid solubility. The compound was found to …

Appropriate Timing Of Fluoxetine And Statin Delivery Reduces The Risk Of Secondary Bleeding In Ischemic Stroke Rats, Maria Helen Harley Balch, Moner A. Ragas, Danny Wright, Kenny Reynolds, Bryce Kerr, Adrian M. Corbett

Neuroscience, Cell Biology & Physiology Faculty Publications

Background: Ongoing clinical trials are testing the effect of fluoxetine delivered post-stroke where a majority of patients are taking statins. This study determined the influence of the timing of administration of fluoxetine and statin on the final infarct volume and the risk of secondary bleeding in an animal model of ischemic stroke.

Methods and findings: Ischemic strokes were induced by endothelin-1 injection into two cortical sites of 10-12 month old female rats, targeting the forelimb motor cortex. Combined medications (5 mg/kg fluoxetine and 1 mg/kg simvastatin) were orally administered either beginning 6-12 hours or 20-26 hours after stroke induction and …

Epigenetics As A Cure For Cancer, Sara Rivka Margolis

The Science Journal of the Lander College of Arts and Sciences

Epigenetics is an emerging research topic that is being tested as a potential cure for cancer. Epigenetics is a non-genetic influence that shapes the phenotype. Epigenetics effects gene expression, but does not cause any changes in the DNA. DNA methylation patterns is one such epigenetic change in the cell that has huge potential for cancer treatment. Scientists have observed that many cancerous genes express signs of either hypermethylation or hypomethylation. The key for the treatment is that epigenetic changes are reversible, which opens the door to potential drugs to cure cancer and other diseases.

ความสัมพันธ์ระหว่างความหลากหลายทางพันธุกรรมบริเวณยีน Pnpla3 และยีน Ncan กับการเกิดมะเร็งตับ, มณีรัตน์ รักษายศ

Chulalongkorn University Theses and Dissertations (Chula ETD)

มะเร็งตับมีอุบัติการณ์เพิ่มขึ้นในประเทศไทย เป็นมะเร็งที่พบบ่อยที่สุดในผู้ชายไทย และเป็นอันดับสามในผู้หญิงไทย การสะสมไขมันในตับเป็นสาเหตุหนึ่งของการเกิดมะเร็งตับ และมีแนวโน้มพบผู้ป่วยจากไขมันสะสมในตับเพิ่มขึ้น ปัจจุบันเชื่อว่าความหลากหลายทางพันธุกรรมของยีนต่างๆ ส่งผลต่อการสะสมไขมันในตับ การวิจัยนี้จึงมีจุดประสงค์เพื่อศึกษาความสัมพันธ์ระหว่างความหลากหลายทางพันธุกรรมของยีน PNPLA3 ตำแหน่ง rs738409 และยีน NCAN ตำแหน่ง rs2228603 ในผู้ป่วยมะเร็งตับ กลุ่มตัวอย่างที่ศึกษาประกอบด้วยกลุ่มผู้ป่วยมะเร็งตับจากไวรัสตับอักเสบบี จำนวน 302 ราย กลุ่มผู้ป่วยมะเร็งตับจากไวรัสตับอักเสบซี จำนวน 153 ราย กลุ่มผู้ป่วยมะเร็งตับจาก ASH/NASH จำนวน 116 ราย และกลุ่มควบคุมสุขภาพดี จำนวน 200 ราย งานวิจัยนี้ใช้เทคนิค TaqMan probe real-time PCR หาความหลากหลายทางพันธุกรรมของยีน ผลการวิเคราะห์ข้อมูลพบว่า ความถี่จีโนไทป์ GG ของยีน PNPLA3 ในกลุ่มผู้ป่วยมะเร็งตับจากไวรัสตับอักเสบบีเป็น 32 (10.6%) กลุ่มผู้ป่วยมะเร็งตับจากไวรัสตับอักเสบซีเป็น 17 (11.1%) กลุ่มผู้ป่วยมะเร็งตับจาก ASH/NASH เป็น 23 (19.8%) และกลุ่มควบคุมสุขภาพดี เป็น 22 (11.0%) โดยจีโนไทป์ GG ในกลุ่มผู้ป่วยมะเร็งตับจาก ASH/NASH สูงกว่ากลุ่มควบคุมสุขภาพดี (OR (95%CI) = 2.24 (1.12-4.47), P=0.022) นอกจากนั้นจีโนไทป์ GG ในกลุ่มผู้ป่วยมะเร็งตับจาก ASH/NASH เมื่อเทียบกับกลุ่มผู้ป่วยมะเร็งตับจากไวรัสตับอักเสบบี และกลุ่มผู้ป่วยมะเร็งตับจากไวรัสตับอักเสบซี พบว่ามีค่าสูงกว่า (OR (95%CI) = 0.45(0.24-0.86) P= 0.015 และ OR (95%CI) = 0.43(0.21-0.9), P= 0.025 ตามลำดับ) ส่วนยีน NCAN ความถี่จีโนไทป์ CC, CT และ TT ในกลุ่มผู้ป่วยมะเร็งตับจากไวรัสตับอักเสบบีเป็น 282 …

Alk Alteration Is A Frequent Event In Aggressive Breast Cancers, Abdul K. Siraj, Shaham Beg, Zeenath Jehan, Sarita Prabhakaran, Maqbool Ahmed, Azhar Hussain, Fouad Al-Dayel, Asma Tulbah, Dahish Ajarim, Khawla S. Al-Kuraya

Centre for Regenerative Medicine & Stem Cell Research

Introduction: Breast cancer is the most common female malignancy worldwide and, despite improvements in treatment modalities, there are increased chances of recurrence and metastasis in a substantial number of cases and it remains one of the major causes of mortality among female cancer patients. Anaplastic lymphoma kinase (ALK) gene has been found to be altered in several solid and hematologic tumors. We aimed to comprehensively study the prevalence of ALK expression, and changes in copy number and translocation in a large cohort of breast cancer cases in a Middle Eastern population.
Methods: ALK protein expression was investigated by immunohistochemistry and …

Overexpression Of Human Cu/Zn Superoxide Dismutase In Mice; The Effect Of Increase Superoxide Scavenging On Autonomic Control Of The Heart., Jeffrey Hatcher

Electronic Theses and Dissertations

Dysregulation of the autonomic cardiovascular control is a complication of diseases including diabetes, hypertension, sleep apnea, and aging. A common factor in these conditions is an increase in reactive oxygen species (ROS) in neural, cardiac, and endothelial tissues. Cu/Zn superoxide dismutase (SOD1) is an intracellular anti-oxidant enzyme that catalyzes dismutation of the superoxide anion (O2.-) to hydrogen peroxide (H2O2). Expression and function of this enzyme are diminished in pathologies that impair cardiovascular autonomic control. This study employed mice overexpressing a transgene for human SOD1 (hSOD1) to determine if its overexpression would alter autonomic regulation of BP, HR, and BRS in …

The Effects Of Home-Based Pilates In Healthy College-Age Females, Betsy Donahoe-Fillmore, Mary Insana Fisher, C. Jayne Brahler

Physical Therapy Faculty Publications

Objectives: To quantify and determine the effects of Pilates on core endurance, hamstring flexibility, balance, body composition/mass and perceived stress level in healthy college age females.

Study Design: Randomized controlled trial design.

Background: Emerging research on the Pilates technique is inconclusive regarding benefits to core endurance, flexibility, balance, body mass, and perceived stress.

Methods and Measures: Female college students (n=57; 18-35 years old) were randomly assigned to a Pilates group, who exercised at home with a DVD, or a control group who did not engage in Pilates practice. Core endurance, hamstring flexibility, balance, body composition and stress measurements were taken …

High Definition Transcranial Direct Current Stimulation Als Mogelijke Hulp Bij Afasie-Herstel, Dirk B. Den Ouden

Communication Sciences and Disorders Faculty Articles and Research

Samenvatting

Nieuwe kennis over het plastische vermogen van het volwassen brein, ook na hersenschade, heeft geleid tot hernieuwde interesse in technieken om deze plasticiteit fysiek te sturen. Een van die technieken is transcranial Direct Current Stimulation (tDCS), waarmee de exciteerbaarheid van hersencellen beïnvloed wordt door een zwakke gelijkstroom door de hersenschors te leiden. Als met deze methode hersenplasticiteit bevorderd en gestuurd kan worden, is het wellicht mogelijk om de uitkomsten van afasietherapie te verbeteren, door het aanpakken van de fysieke bron van de stoornis. Dit artikel behandelt kort de geschiedenis en de werking van tDCS, alvorens een overzicht te geven …

Protective Effects Of Erdosteine, Vitamin E, And Vitamin C On Renal Injury Induced By The Ischemia-Reperfusion Of The Hind Limbs In Rats, Rana Sirmali, Abdullah Armağan, Faruk Öktem, Efkan Uz, Aynur Kirbaş, Soner Dönmez, Haci Ramazan Yilmaz, Mesrur Selçuk Silay, Mehmet Sirmali

Turkish Journal of Medical Sciences

To compare the protective efficacy of erdosteine and vitamins C and E against renal injury caused by hind limb ischemia-reperfusion (I/R). Materials and methods: Rats were split into 4 groups: group I as the control, group II as I/R, group III as I/R + erdosteine, and group IV as I/R + vitamins C and E. Superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) activities and malondialdehyde (MDA) tissue levels were determined. Results: MDA levels were found comparable with the control group in groups II and III. However, they were considerably decreased in group IV when compared to group II …

Effects On Quinolone Resistance Due To The Biofilm Formation Activity In Ureaplasma Urealyticum, Chunyan Feng, Ya Huang, Yunsong Yu, Guorong Duan, Yuying Dai, Ke Dong, Qingtian Li

Turkish Journal of Medical Sciences

To detect the effects on quinolone resistance due to biofilm formation activity in Ureaplasma urealyticum. Materials and methods: An improved checkerboard dilution susceptibility test was used to analyze the quinolone resistance of clinical Ureaplasma isolates and a postbiofilm formation susceptibility assay was used to compare the effects on the quinolone susceptibility between pre- and postbiofilm formations. Several important functional gene expressions were detected to evaluate their roles in the process of the quinolone resistance mechanism. Results: The quinolone-resistant isolates produced more biofilms than the sensitive isolates. In both the quinolone-resistant and -sensitive groups, the minimal inhibitory concentrations after biofilm formation …

Distribution Of Viral Genotypes And Extrahepatic Manifestations In Patients With Chronic Hepatitis C In Eastern Turkey, Si̇bel İba Yilmaz, Serpi̇l Erol, Ahmet Özbek, Mehmet Parlak

Turkish Journal of Medical Sciences

To investigate the distribution of viral genotypes, the extrahepatic manifestations, and the relationship between genotypes and extrahepatic manifestations in patients with chronic hepatitis C. Materials and methods: The study included 62 treatment-naive patients with chronic hepatitis C infection. Genotype determination was performed by DNA sequencing analysis. To investigate extrahepatic manifestations, the patients' data, recorded prospectively during the pretreatment period, were analyzed. Results: Genotype determination was successful in 74.2% of the cases. All patients were infected with hepatitis C virus (HCV) genotype 1b. At least 1 extrahepatic manifestation was identified in 37.1% of the patients. The most frequent clinical manifestations were …

A Molecular Epidemiological Investigation Of Multistate Outbreaks Of Salmonella Enteritidis From Clinical And Environmental Samples In Turkey, 2000-2010, Sümeyra Acar, Belkis Levent, Ekrem Atalan

Turkish Journal of Medical Sciences

To analyze interrelated Salmonella Enteritidis strains isolated in Turkey. Fifty-five S. Enteritidis surveillance strains were isolated from human feces and environmental samples from different regions in Turkey between 2000 and 2010. Materials and methods: Clinical isolates were selected from different outbreaks in the Turkish National Reference Laboratory. All isolates were submitted to antimicrobial susceptibility test, plasmid profile analysis, and XbaI-digested pulsed-field gel electrophoresis. Results: The strains were scanned against 20 antibiotics and for 3 of them (amikacin, ciprofloxacin, gentamicin), all strains were found to be sensitive. Five isolates had no plasmid. Most of test strains carried the 57-kb plasmid in …

Prognostic Value Of Hemostasis-Related Parameters For Prediction Of Organ Dysfunction And Mortality In Sepsis, Dunja Mihajlovic, Dajana Lendak, Gorana Mitic, Tatjana Cebovic, Biljana Draskovic, Aleksandra Novakov, Snezana Brkic

Turkish Journal of Medical Sciences

Clinical manifestations of sepsis are not caused directly by the invading pathogens, but rather mostly by systemic inflammation that leads to activation of the coagulation system. The aim of this study was to determine whether levels of hemostasis-related parameters measured in intensive care unit admissions are associated with mortality and severity in patients with sepsis. Materials and methods: Eighty-five patients who fulfilled criteria for a diagnosis of sepsis were included in our study. Platelet count, activated partial thromboplastin time (aPTT), prothrombin time (PT), thrombin time, D-dimer, and fibrinogen levels were determined within the first 24 h from sepsis onset. Differences …

Clinicopathological Importance Of Ki-67, P27, And P53 Expression In Gastric Cancer, Muhammet Çalik, Eli̇f Demi̇rci̇, Eren Altun, İlknur Çalik, Özge Beyza Gündoğdu, Nesri̇n Gürsan, Betül Gündoğdu, Mevlüt Albayrak

Turkish Journal of Medical Sciences

To assess the prognostic value of Ki-67, p27, and p53 immunoreactivity in human gastric cancer. Materials and methods: A total of 84 patients with gastric cancer participated in our study. We categorized tumors as intestinal and diffuse types, with reference to Lauren's classification. Ki-67, p27, and p53 immunoreactivity were correlated with patient's age, tumor type, grade, lymph node status, extent of invasion, tumor-node-metastasis (TNM) stage, and survival. Results: Decreased expression of p27 (50% positivity of cells) were determined in 41 (48.8%) and 29 (36.9%) tumor specimens, respectively, and were connected with both the TNM stage (P = 0.007 and P …

Evaluation Of Autoinflammatory Disease Genes In Nasal Polyposis, Sabri̇ Köseoğlu, Kürşat Murat Özcan, Aykut İki̇nci̇oğullari, Mehmet Ali̇ Çeti̇n, Tali̇h Özdaş, Si̇bel Özdaş, Serdar Ensari̇, Haci Hüseyi̇n Dere

Turkish Journal of Medical Sciences

To investigate cold-induced autoinflammatory syndrome 1 (CIAS1) gene polymorphisms that cause autoinflammatory diseases in patients with nasal polyposis (NP). Materials and methods: The study included 30 patients diagnosed with NP and 30 healthy age-matched individuals as a control group. CIAS1 polymorphisms were assessed by DNA sequence analysis. Patients with nasal polyps and the control group were compared in terms of gene polymorphisms. Each of the 8 polymorphisms of the CIAS1 gene was analyzed separately in the patient group. Results: The most frequently observed polymorphisms in the patient group were c.732G > A in 83%, c.663C > T in 23%, and c.1308C > A …

Stress-Responsive Factor Regulation In Patients Suffering From Type 2 Diabetes And Myocardial Infarction, Sobia Mushtaq, Tahir Ali, Farhana Altaf, Muhammad Abdullah, Iram Murtaza

Turkish Journal of Medical Sciences

Pro-free radical oxidative stresses, as well as regulatory factors, are believed to be the key players in the development of diabetes and heart-related disorders such as myocardial infarction. The aim of the present study was to highlight the role of oxidative stress-responsive factors (reactive oxygen species [ROS], super oxide dismutase [SOD], and calpain-1) in type 2 diabetes and myocardial infarction. Materials and methods: A total of 100 type 2 diabetes patients with myocardial infarction and 50 normal individuals were selected for this analysis. The levels of ROS and activities of SOD in the serum were determined. Serum calpain-1 expression was …

Hypo- And Hypervolemic Edema In Children With Steroid Sensitive Nephrotic Syndrome, Mehmet Aki̇f Büyükavci, Mahmut Çi̇vi̇li̇bal, Murat Elevli̇, Hati̇ce Ni̇lgün Selçuk Duru

Turkish Journal of Medical Sciences

The mechanism of edema formation in nephrotic syndrome is still poorly understood. We aimed to evaluate the volume status in children with steroid-sensitive nephrotic syndrome (SSNS) and to emphasize the importance of echocardiography in demonstrating of volume changes. Materials and methods: Thirty-two SSNS patients and 30 healthy children were enrolled in this study. The volume statuses of patients were evaluated by clinical and laboratory features, including fractional sodium excretion (FENa) and distal sodium/potassium exchange (UK/UNa+K ratio). Inferior vena cava collapsibility index (IVCCI), left atrial diameter (LAD), aortic diameter (AD), and left ventricular mass index (LVMI) were measured using conventional echocardiographic …

The Long-Term Changes In Pain-Related Symptomatology Of Inguinal Hernia Following 2 Different Herniorrhaphy Techniques, Sali̇h Erpulat Özi̇ş, Işin Ünal Çevi̇k, Hati̇m Yahya Uslu, Süleyman Özdemi̇r, Kami̇l Gülpinar, Hasan Turgut Aydin

Turkish Journal of Medical Sciences

To compare the changes in pain-related symptoms of inguinal hernias from initial admission to postoperative month 6 following 2 herniorrhaphy techniques. Materials and methods: Patients with unilateral inguinal hernias were scheduled for either Lichtenstein or self-gripping polypropylene mesh repair. Patients were preoperatively evaluated with a visual analog scale (VAS) and a Turkish version of the Douleur Neuropathique 4 (DN4) questionnaire and the complaints related to pain were noted. After surgery, patients were discharged without early complications. Patients were reassessed at postoperative month 6. The late-term complaints of pain as well as neurological findings were evaluated using the VAS and the …

Structural Chromosomal Abnormalities In Couples With Recurrent Abortion In Egypt, Nagwa E.A. Gaboon, Ahmed Ramy Mohamed, Solaf M. Elsayed, Osama K. Zaki, Mohamed A. Elsayed

Turkish Journal of Medical Sciences

To evaluate the incidence of chromosomal abnormalities in couples who experience recurrent abortion and identify additional factors that may be predictive of abortion, such as parental age and unfavorable obstetric or abnormal semen analysis. Materials and methods: The present study examined 125 couples who had experienced recurrent abortion. All subjects provided a detailed personal medical history and ancestral history and underwent a physical examination. Women in the study group underwent biochemical testing and pelvic ultrasound examinations, and men underwent a semen analysis. Results: Among the 125 couples tested, 8 couples (6.4%) displayed a balanced translocation, among which 7 (5.6%) showed …

Aberrant Expression Of Leukemia Inhibitory Factor Receptor (Lifr) And Leukemia Inhibitory Factor (Lif) Is Associated With Tubal Pregnancy Occurrence, Yong Li, Lizhou Sun, Denmei Zhao, Jun Ouyang, Mei Xiang

Turkish Journal of Medical Sciences

Tubal pregnancy is a major cause of maternal death in the first trimester and exploration of its underlying molecular mechanism is of great importance. This study aimed to explore the association of tubal pregnancy with leukemia inhibitory factor (LIF) and leukemia inhibitory factor receptor (LIFR) expression in oviduct tissues. Materials and methods: Immunohistochemistry was performed to probe the differential expression of LIF and LIFR in oviduct tissues among a control group (including NP group, n = 11; and IP group, n = 12), tubal pregnancy group (Ect-N group, n = 31; and Ect-A group, n = 40), and chronically inflamed …

The Effect Of Extracorporeal Shock Wave Lithotripsy On Distribution Of Interstitial Cells Of Cajal In Rabbit Renal Pelvis And Proximal Ureter, Özlem Boybeyi̇, Mi̇ne Fedakar Şenyücel, Ebru Şebnem Ayva, Tutku Soyer, Mustafa Kemal Aslan, Mehmet Murad Başar, Ahmet Murat Çakmak

Turkish Journal of Medical Sciences

An experimental study was performed to evaluate the effect of extracorporeal shock wave lithotripsy (ESWL) on the distribution of interstitial cells of Cajal (ICC) in rabbit renal pelvis and proximal ureter. Materials and methods: Six New Zealand rabbits were included. Right kidneys were exposed to a total of 3000 shock waves (14 kV) by using an electrohydraulic-type ESWL device. Right sides were allocated as the ESWL group (EG, n = 6) and left sides as the control group (CG, n = 6). Tissues were harvested on day 7. Tissues were examined histopathologically for the presence of edema, inflammation, congestion, hemorrhage, …

Comparison Of Perioperative Oxidative Stress In Endovascular And Open Repair Of Abdominal Aortic Aneurysm, Orhan Veli̇ Doğan, Ümi̇t Menteşe, Süheyla Doğan, Sefer Usta, İbrahi̇m Turan, Ergün Hali̇loğlu, Süleyman Caner Karahan

Turkish Journal of Medical Sciences

To investigate the differences in perioperative oxidative stress (OS) in abdominal aortic aneurysm (AAA) patients treated with either endovascular repair (EVAR) or open repair (OR). Materials and methods: Twenty patients (11 OR, 9 EVAR) treated for AAA with no known malignant or inflammatory disease and an aneurysm diameter of over 5 cm and no rupture were included in the study. Blood samples were obtained preoperatively, during aortic occlusion, and 1 h and 24 h after reperfusion. Total antioxidant status (TAS), total oxidative stress (TOS), and malondialdehyde (MDA) levels were measured and oxidative stress index (OSI) was calculated. Results: OSI at …

Effect Of Keratinocyte Growth Factor On Growth And Transdifferentiationof Primary Alveolar Epithelial Type Ii Cells, Xiuxiang Liu, Zhichun Feng, Chunmei Tian, Xiangyong Kong, Yumei Wu, Changjun Lv

Turkish Journal of Medical Sciences

Background/aim: To investigate the effects of keratinocyte growth factor (KGF) on the growth and transdifferentiation of primary alveolar epithelial type II cells (AECIIs). Materials and methods: The number of primary AECIIs, their viability, and cell cycle and apoptosis were studied under KGF treatment using a hemocytometer, trypan blue exclusion, and flow cytometry, respectively. Positive expressions of surfactant protein C (SP-C), aquaporin 5 (AQP5), and thyroid transcription factor 1(TTF-1) were examined with indirect immunofluorescence; mRNA levels of SP-C, AQP5, and TTF-1 were determined by polymerase chain reaction. Results: In response to KGF treatment, cell numbers were significantly increased, more cells were …

Protective Effect Of \Beta-Glucan On Acute Lung Injury Induced Bylipopolysaccharide In Rats, Meryem Iraz, Mustafa Iraz, Mukaddes Eşrefoğlu, Mehmet Şeri̇f Aydin

Turkish Journal of Medical Sciences

Background/aim: Lipopolysaccharide (LPS)-induced endotoxemia can cause serious organ damage such as acute lung injury and death by triggering the secretion of proinflammatory cytokines and acute-phase reactants. The goal of this study was to evaluate the effects of \beta-glucan on inflammatory mediator levels and histopathological changes in LPS-induced endotoxemia. Materials and methods: Forty-seven male Wistar albino rats were randomly allocated into four groups as follows: control group, LPS group (10 mg/kg LPS), LPS + \beta-glucan group (100 mg/kg \beta-glucan before LPS administration), and \beta-glucan group. Twelve hours after LPS administration, lung and serum samples were collected. Concentrations of IL-6, IL-8, C-reactive …