Medicine and Health Sciences Commons^™

Unusual Lymphomas Developing In Chronic Lymphocytic Leukemia, Sundara B. K. Raman, Sheikh M. Saeed, Joseph P. Abraham

Henry Ford Hospital Medical Journal

We report three patients with chronic lymphocytic leukemia (CLL) who developed malignant lymphomas of unusual character and modes of presentation. Two of the patients had received low doses of chlorambucil for several years before they developed malignant lymphoma, diffuse, large cell type (LCL). In one of these patients LCL manifested as a grossly evident osteolytic lesion. In the second patient LCL developed initially as a localized lesion in the iliac bone. Both patients died within a few weeks after LCL was diagnosed. The third patient, who was found to have CLL during a routine examination, did not receive any therapy …

T-Cell Lymphoma In Renal Transplant Recipient, Koichi Maeda, Leslie Bricker, Chan K. Ma, Michael J. Deegan

Henry Ford Hospital Medical Journal

A 35-year-old woman, who had a renal transplant flve years ago, developed malignant lymphoma of the mediastinum. The lymphoma was of lymphoblastic type and had T-cell immunophenotype. Most transplant-related lymphomas are of B-cell type. T-cell lymphoma in this setting is extremely rare, and the mechanism of development may be different from that of B-cell lymphomas.

Obstructing Giant Pseudopolyposis Of The Colon In Ulcerative Colitis, Roberta E. Sonnino, Farouck N. Obeid

Henry Ford Hospital Medical Journal

Pseudopolyposis of the colon is a well-recognized local complication of ulcerative colitis. Giant pseudopolyposis is uncommon, and its presentation as a complete obstruction of the colon is a rare occurrence. We report such a case, where retrograde flow of barium on double-contrast barium enema was completely obstructed at midtransverse colon. After resection by total abdominal colectomy, pathology examination ofthe specimen confirmed massive pseudopolyposis without malignancy. A brief review of the pathology and current literature is presented.

Ocular Irrigating Solutions: A Comparison Between Balanced Salt Solution And L-410 (Po-Eis), Philip C. Hessburg, Paul M. Johnston

Henry Ford Hospital Medical Journal

The ability of two ocular irrigating solutions to reduce postoperative corneal edema after intracapsular cataract extraction was compared using ultrasonic pachymetry. All patients had anterior chamber intraocular lenses implanted. The solutions were balanced salt solution and solution L-410 (PO-EIS), an eye irrigation solution containing dextran 40 and bicarbonate. Corneas irrigated with either solution showed no significant difference in postoperative edema. The use of L-410 (as an alternative to balanced salt solution) may not be necessary in intracapsular cataract extraction.

Melvin A. Block, Md, Gary B. Talpos

Henry Ford Hospital Medical Journal

No abstract provided.

Book Review: Guides To Clinical Aspiration Biopsy: Thyroid, John E. Brunner

Henry Ford Hospital Medical Journal

No abstract provided.

Hyperosmotic Modification Of Brain Extracellular Space And Blood-Brain Barrier In The Rat As Observed By Electron Microscopy, Susan A. Meacham

Loma Linda University Electronic Theses, Dissertations & Projects

The highly selective permeability of cerebral blood vessels, termed the blood-brain barrier, is due to endothelial tight junctions (zonulae occludentes). Hyperosmotic solutions reversibly open the blood-brain barrier. The purpose of this study is to determine the immediate effect of hyperosmotic arabinose perfusion upon the cerebrovascular endothelium, the morphology of the cerebral cortex, and the cortical extracellular space.

Twelve adult male Sprague-Dawley rats were separated into three groups of four animals each: control, intermediate, and extreme. Controls were perfused with a Tyrode's solution prewash followed by trialdehyde fixative. The intermediate group received a prewash of 740 mmol/kg arabinose followed by the …

Time Delayed Protein Complementation, Diana L. Lakusta

Loma Linda University Electronic Theses, Dissertations & Projects

The time delayed complementation of plant proteins was tested in weanling Sprague-Dawley male rats by measuring growth rates as well as plasma and tissue free amino acids. Diets of pinto beans with rice, corn or wheat were pair-fed in combination or alternating 4 times a day at 6 and 11 am, and 4 and 9 pm for 4 weeks. Plasma, liver and muscle samples were obtained 2 hours postprandial, a time determined most suitable for sacrifice, and analyzed for free amino acids via ion-exchange chromatography. There were no significant differences in growth of rats fed combination or alternating diets.during week …

Santa Clara Magazine, Volume 30 Number 1, Fall 1987, Santa Clara University

Santa Clara Magazine

10 - CAN THERE BE DISSENT IN CATHOLIC UNIVERSITIES? SCU's president makes clear where the University stands on this important issue. By William J. Rewak, S.J.

17 - WHAT'S AHEAD IN THE WORLD OF HIGH TECH? Former Business ~ek reporter Jack Wilson looks to the future of Silicon Valley.

21 - GREEKS ON CAMPUS-HERE TO STAY? What makes sororities and fraternities appealing to SCU undergraduates?

28 - GLASS IMAGE Tom Kelly '72 unlocks memories of classmate Charlie Glass after Glass's abduction last June.

30 - THE AGE OF AQUARIUS REVISITED Six members of the class of '67 talk about their …

Isolation Of A Cdna Clone For The Human Laminin-B1 Chain And Its Gene Localization, Michael Jaye, William Modi, George A. Ricca, Robert Mudd, Inc-Ming Chiu, Stephen J. O'Brien, William N. Drohan

Biology Faculty Articles

A cDNA clone encoding the B1 chain of human laminin has been isolated from a human endothelial cell cDNA library. With use of this probe and a panel of rodent/human somatic-cell hybrids and in situ hybridization, the gene for the human laminin-B1 chain has been localized to chromosome 7, band q31.

Synthesis And Characterization Of N-Alkyl-Adenosine-5'-Diphosphates, Han-Ping Huang

Chemistry & Biochemistry Theses & Dissertations

n-Alkylphosphates and adenosine derivatives have been observed to be competitive inhibitors of coenzyme molecules in many NAD⁺ requiring enzymes. In earlier studies, adenosine derivatives such as AMP, ADP and ADP-ribose were found to be coenzyme competitive inhibitors of the Lactate Dehydrogenase catalyzed reaction. However, n-alkylphosphates, to the contrary, + were found to be noncompetitive inhibitors of NAD⁺.

Furthermore, it was observed previously in Dr. Yuan's laboratory that the effect of n-octylphosphate on the LDH catalyzed reaction was changed from noncompetitive to competitive in the presence of low concentration of AMP. Rossmann et al. reported that the binding …

Investigation Of Complex Formation By Oligomers Of Cytosine And Guanosine, Steven Roberts Davis

Chemistry & Biochemistry Theses & Dissertations

Duplex formation between oligo(C:G) _n where n=3 to 4 was shown not to occur under conditions favorable for duplex formation between poly G and poly C. Instead, a stable guano sine self-structure was found to form which a T_m of 50°C for (Gp)₃ and 80°C for (Gp)₄ at strand concentrations of 10^-5M in 1M NaCl. Neither a duplex nor a self-structure formed in the absence of salt.

Oligomers of guanosine and cytosine were obtained by basic hydrolysis and separated according to chain length using DEAE Sephadex column chromatography. Separation of cytosine oligomers with chain lengths …

Insulin And Glucagon Responses To Dietary Protein In Diabetic And Nondiabetic Men, Monica L. Blomstedt

Loma Linda University Electronic Theses, Dissertations & Projects

Five men with non-insulin-dependent diabetes mellitus and fourteen nondiabetic men, were given test meals with soy protein, casein or additional carbohydrate in place of protein. Blood levels of insulin, glucagon, glucose and lipids were measured at fasting, one-half hour and two hours postprandial. To all meals, the nondiabetic subjects responded with a quick rise of the insulin level which decreased at two hours. In contrast, the diabetics showed a significantly lower insulin response at one-half hour (p < 0.05), but a tendency to higher levels at two hours. The same pattern was observed in the insulin to glucagon ratio. No significant difference between the meals was found in regard to insulin or glucagon responses, suggests that a sustained, although delayed postprandial insulin response, may be a precursor to the higher cholesterol and increased risk for cardiovascular disease among diabetics.

Effect Of Disulfiram On The Urinary Excretion Of The Serotonin Metabolites 5-Hydroxytryptophol And 5-Hydroxyindoleacetic Acid In Problem Drinkers, Mark D. Harding

Loma Linda University Electronic Theses, Dissertations & Projects

Problem drinkers (N=7) were given 500mg disulfiram daily for one week. Urine was collected before and after disulfiram intake. 5-Hydroxytryptophol (5-HTOL) was determined by a modification of the method of Davis et al. and adapted to high-performance liquid chromatography (HPLC) with an electrochemical detector setting of 0.75V. 5-Hydroxy-indoleacetic acid (5- HIAA) was determined using the HPLC method of Shihabi et al. (1980). Urinary excretion of 5-HTOL in ug/gm creatinine before and after disulfiram was 25.4± 3.3 and 110.5± 27.1 respectively, p < 0.02. 5-HIAA in mg/gm creatinine before and after disulfiram was 4.9±0.9 and 3.6±0.8 respectively, p > 0.16. The data shows that disulfiram administration alters serotonin metabolism resulting in increased 5-HTOL which is known to increase …

The Correlation Of Physiological Responses To Verbal Behavior During Environmental Stimuli Exposure, Cynthia Lou Baker

Graduate Theses

The study was designed to investigate the hypothesis that various environmental stimuli would elicit specific individual responses, as reflected in autonomic patterns. It then became the aim of the study to see whether the stimuli which controlled the physiological pattern would also control the verbal response. Three female and two male students volunteered for the single-subject experiment, where they were exposed to a 15-slide sequence of environment stimuli. Results indicated that when allowed to verbally respond without rating restrictions, responses varied greatly, limiting the possibilities of comparison. In kind, the physiological responses exhibited great variability. Results also supported the position …

Distribution Of Vertebrates Of The Bighorn Canyon National Recreation Area, Stanley H. Anderson, Wayne A. Hubert, Craig Patterson, Alan J. Redder, David Duvall

Great Basin Naturalist

During a survey of the vertebrates in the Bighorn Canyon National Recreation Area, 46 mammal, 210 bird, 9 reptile, 5 amphibian, and 28 fish species were found. Habitat structure and moisture were two environmental variables associated with species numbers across this 1,820-ha area. Eight distinct habitats were evaluated. In coniferous forests, 27% of the birds and 54% of the mammals were observed. Sagebrush/grasslands and upland shrublands were very arid and had relatively few vertebrates. Five introduced game birds occurred in sagebrush habitat. Riparian and wetland habitats had the largest number of unique vertebrates, 38 and 77 respectively. The establishment of …

A Novel Form Of Human Polymorphism Involving The Hdhfr-Ψ1 Pseudogene Identifies Three Rflps, Nicholas P. Anagnou, S. E. Antonarakis, Stephen J. O'Brien, Arthur W. Nienhuis

Biology Faculty Articles

No abstract provided.

Thyroid C-Cell Hyperplasia And Micronodules In Close Relatives Of Men-2 A Patients: Pitfalls In Early Diagnosis And Reevaluation Of Criteria For Surgery, Cees J. M. Lips, John R. Leo, Marianne J. H. Berends, Werner H. Minder, A. P. Roeland Blok, Rolf A. Geerdink, Wil H. L. Hackeng, Jan M. M. Roelofs, Hans F. A. Vasen, Jan K. Vette

Henry Ford Hospital Medical Journal

In a large family with multiple endocrine neoplasia rype 2 A (MEN-2 A), 20 patients were identified by the diagnosis of medullary thyroid carcinoma (MTC) and/or pheochromocytomas. Another five subjects had neck surgery on the basis of slightly increased results of a C-cell provocative test. Retrospectively, however, although the immunohistochemical diagnosis of C-cell hyperplasia was confirmed, the diagnosis of MEN-2 was doubtful in these five subjects, and the C-cell hyperplasia observed was probably within the limits of normal variation. The occurrence of C-cell hyperplasia in a normal population was investigated by a C-cell provocative test, as well as in random …

Santa Clara Magazine, Volume 29 Number 4, Summer 1987, Santa Clara University

Santa Clara Magazine

10 - ALASKA DIARY From the journal Bill Regan kept on his 1931 expedition to Alaska with Father Hubbard. By William V. Regan. Edited by Peg Major

18 - THE U.S. AND THE VATICAN An excerpt from Eric Hanson's new book sets the stage for the pope's September visit. By Eric O. Hanson

21 - MR. ESPY GOES TO WASHINGTON Mike Espy '78 (JD) is Mississippi's first black congressman since Reconstruction. By Carla Hall

24 - BURIED TREASURE For every child God gave them, Kathy and Allen Hayes '66 promised to adopt another. By Allen and Kathy Triplett Hayes '66

Reactions Of Organic N-Chloramines In The Gastric Fluid Of The Rat, Kathryn E. Mazina

Chemistry & Biochemistry Theses & Dissertations

Using chlorine as a drinking water disinfectant may have potential health effects due to its reactivity with organic amino nitrogen compounds found in the stomach. Organic N-chloramines have been shown to form in the stomachs of laboratory rats. The possible reactions of N-chloramines in the stomach fluid were examined in this study using a model radiolabeled N-chloramine. ³⁶Cl-N-Chloropiperidine, was synthesized and purified to remove ³⁶Cl-chloride. Stomach fluid was obtained from Sprague-Dawley rats which had been first fasted for 24 or 48 hours and then administered 3 mL of deionized water. Different concentrations of radiolabeled chloramine were reacted with …

Plasma And Tumor Levels Of Somatostatin (Srif) And Somatostatin Immunochemistry In Medullary Thyroid Carcinoma: Apparently Discrepant Preliminary Results, B. Franc, J. A. Chayvialle, E. Modigliani, C. Calmettes, B. Caillou, N. Dutrieux-Berger, C. Houdent, M. Kujas

Henry Ford Hospital Medical Journal

Eighteen cases of medullary thyroid carcinoma (MTC) (11 hereditary) had parallel measurements of basal plasma levels of somatostatin (SRIF), levels of SRIF extracted from fresh tumor tissue (radioimmunoassay), and immunohistochemistry (peroxidase-antiperoxidase method) using an anti-SRIF antibody on paraffin sections. Immunohistochemistry disclosed some cell positivity in nine cases (50%). Plasma levels of SRIF were above normal (> 50 pg/mL expressed in terms of the 14 amino acid SRIF peptide) in only two of 12 patients. Extraction of fresh tumor tissue detected SRIF in 60% of our cases: two patients > l.200pg/mg (equivalents 14). with the other positive values ranging between 13 and …

Impact Of Prospective Screening For Multiple Endocrine Neoplasia Type 2, Robert F. Gagel, Armen H. Tashjian Jr., Tim Cummings, Nick Papathanasopoulos, Seymour Reichlin

Henry Ford Hospital Medical Journal

Prospective annual screening for hereditary medullary thyroid carcinoma (MTC) in the J-kindred, currently a 117-member family with multiple endocrine neoplasia type 2A, began in 1969. During the initial screening, 12 patients were found to have MTC. Subsequent screening has detected C-cell abnormalities (C-cell hyperplasia or microscopic MTC) in 22 of 23 addilional family members thyroidectomized for abnormal calcium- or pentagastrin-provocative calcitonin (CT) test results. Seven of the initial 12 patients thyroidectomized in 1970 to 1971 and 19 of 23 individuals thyroidectomized since 1971 remain disease-free by all criteria; three patients thyroidectomized since 1971 have had clearly abnormal serum CT measurements …

Subtotal Adrenalectomy In Multiple Endocrine Neoplasia Type 2, Bertil Hamberger, Margareta Telenius-Berg, Bjorn Cedermark, Staffan Grondal, Bengt-Goran Hansson, Sigbritt Werner

Henry Ford Hospital Medical Journal

We report two patients in whom pheochromocytoma was treated by subtotal adrenalectomy leaving a rim of vascularized cortical tissue in situ. Both patients are doing well without cortisol supplementation although they have subnormal cortisol responses to ACTH stimulation.

Application Of Minisatellite Dna Probes To Linkage In Men-2, Bruce A. J. Ponder, Alec J. Jeffreys, Nicola E. Hartley, Clare Carter, Douglas F. Easton, Hakan Telenius, Margareta Telenius-Berg

Henry Ford Hospital Medical Journal

We describe the potential benefits and the limitations of the use of highly polymorphic minisatellite DNA probes for genetic linkage analysis of multiple endocrine neoplasia type 2A (MEN-2 A). The advantage of these probes is that up to 34 loci can be examined in a single experiment, and since the loci are highly polymorphic, almost every individual in every family is informative. The disadvantage is that the DNA fragment lengths of the alleles at any given locus differ from one family to another, so that families cannot be combined, and large single sibships are needed to obtain significant linkage data. …

One Large Kindred Excludes A Locus For Multiple Endocrine Neoplasia Type 2a From About 25% Of The Human Autosomal Genetic Map, Andrew J. Pakstis, Judith R. Kidd, Carmela M. Castiglione, Beth A. Pletcher, Patricia D. Murphy, Lindsay A. Farrer, Myron Genel, Kenneth K. Kidd

Henry Ford Hospital Medical Journal

This report presents pairwise linkage results from our search for the locus of the gene (MEN2A) for the multiple endocrine neoplasia type 2A (MEN-2A) syndrome in one large kindred (the N kindred), clearly segregating for an autosomal dominant form. About 25% of the autosomal genome is excluded when these new results are combined with those we have published previously. The genetic markers employed are distributed across at least 19 of the 22 autosomes. Seven genetic markers whose chromosomal locations are not yet established have also been studied.

Immunohistochemistry In Medullary Thyroid Carcinoma: Prognosis And Distinction Between Hereditary And Sporadic Tumors, B. Franc, B. Caillou, A. M. Carrier, N. Dutrieux-Berger, J. Floquet, M. Houcke, E. Justrabo, F. Lange, A. Pages, C. Rigaud, A. Schwartz, M. Viennet, M. F. Lebodic

Henry Ford Hospital Medical Journal

In this retrospective study of 68 cases of medullary thyroid carcinoma (MTC), morphological features were studied, which could be helpful in prognosis and in the distinction between hereditary and sporadic forms of the disease. Necrosis within the tumors seemed to carry a poor prognosis. Bilateral MTC. unquestionable C-cell hyperplasia, glandular or follicular pattern, and thyroglobulin-positive cells seemed to be more prevalent in hereditary forms of the disease. A smaller proportion of calcitonin-immunoreactive cells were encountered in hereditary tumors than in sporadic tumors.

Different Mechanisms Of Calcitonin, Calcitonin Gene-Related Peptide, And Somatostatin Regulation By Glucocorticoids In A Cell Culture Of Human Medullary Thyroid Carcinoma, Gilbert J. Cote, Robert F. Gagel

Henry Ford Hospital Medical Journal

We have employed the TT cell line, a model for the human medullary thyroid carcinoma cell, lo study the regulation of peptide hormone production by glucocorticoids. Complementary DNA probes were used to measure the calcitonin (CT), CT gene-related peptide (CGRP), and somatostatin (SRIF) mRNA levels. Dose-response experiments in serum-free medium showed that dexamethasone (six-day treatment) lowered somatostatin (to 1% of basal) and CGRP mRNA (to 50% of basal) and stimulated CT mRNA (threefold to thirteenfold) with a half-maximal effective concentration of 10⁻⁸ M. Time course studies for cells continuously exposed to 10⁻⁶ M dexamethasone showed a rapid (within …

Front Matter

Henry Ford Hospital Medical Journal

No abstract provided.

The Second International Workshop On Multiple Endocrine Neoplasia Type 2 Syndromes, Bruce A. J. Ponder, Charles E. Jackson

Henry Ford Hospital Medical Journal

No abstract provided.

Screening In Medullary Thyroid Carcinoma, C. Calmettes

Henry Ford Hospital Medical Journal

The four-year experience of the French collaborative study on medullary thyroid carcinoma (MTC) has permitted improvement in family screening by a common discussion of data and a network of coordinators throughout France involved in the detection of new cases. Investigations have been directed at improving the discrimination between sporadic and hereditary forms of MTC and between individuals who have or have not inherited the disease within families.