Medicine and Health Sciences Commons^™

Ua66/14/4 Kentucky Partnership Newsletter, Vol. Ii, No. 7, Kentucky Partnership For Farm Family Health & Safety

University Organizations

Newsletter created by and about the Kentucky Partnership for Farm Family Health & Safety.

A Novel Translational Regulation Function For The Simian Virus 40 Large-T Antigen Gene., Prithi Rajan, Sathyamagalam Swaminathan, Jiyue Zhu, Charles N. Cole

Dartmouth Scholarship

Cells use the interferon-induced, double-stranded-RNA-dependent protein kinase PKR as a defense against virus infections. Upon activation, PKR phosphorylates and thereby inactivates the protein synthesis initiation factor eIF-2, resulting in the cessation of protein synthesis. Viruses have evolved various strategies to counteract this cellular defense. In this paper, we show that simian virus 40 (SV40) large-T antigen can antagonize the translational inhibitory effect resulting from the activation of PKR in virus-infected cells. Unlike the situation with other virus-host cell interactions, SV40 large-T antigen does not block the activation of PKR, suggesting that SV40 counteracts the cellular antiviral response mediated by PKR …

Increased Stem Cell Factor Release By Hemangioma-Derived Endothelial Cells, C. J. Meininger, Shannon Brightman, K. A. Kelly, B. R. Zetter

Biology Faculty Publications

Background: Capillary hemangiomas, the most common tumors in young children, consist of proliferating capillary vessels and endothelial cells. These tumors also contain large numbers of mast cells, compared with the normal surrounding skin or tissue. We have recently shown that stem cell factor (SCF), the gene product of the murine steel locus, can act as a chemoattractant for mast cells. In this study, we investigated whether SCF might be involved in the recruitment and maintenance of mast cells in hemangiomas. Experimental Design: Cultured endothelial cells derived from a murine hemangioma were compared with normal vascular endothelial cells for the ability …

Biotransformation Of Bile Acids, Cholesterol And Steroids. Chapter 13 In: The Ecology And Physiology Of Gastrointestinal Microbes. Vol. 1, New York, Chapman And Hall., Stephen Baron, Phillip B. Hylemon

Biology Faculty Scholarship

No abstract provided.

Santa Clara Magazine, Volume 37 Number 1, Winter 1995, Santa Clara University

Santa Clara Magazine

6 - THE JESUIT CASE DECLASSIFIED Secret documents reveal what the United States knew.... and what it failed to do about the Jesuit murders in El Salvador. By Peter Kornbluh

10 - EASTERN ENLIGHTENMENT SCU's new religion chair draws from her latest book to discuss how Christians can derive a greater understanding of their own faith through an appreciation of Buddhism. By Denise Lardner Carmody

13 - WASTE NOT As part of SCU's Institute on Ecology, students, faculty, and staff assess how well the University is using its own environmental resources.

17 - THE DOMESTIC CHURCH MEETS THE PARISH CHURCH …

The Three Rs: The Way Forward, Michael Balls, Alan M. Goldberg, Julia H. Fentem, Caren L. Broadhead, Rex L. Burch, Michael F.W. Festing, John M. Frazier, Coenraad F.M. Hendriksen, Margaret Jennings, Margot D.O. Van Der Kamp, David B. Morton, Andrew N. Rowan, Claire Russell, William M.S. Russell, Horst Spielmann, Martin Stephens, William S. Stokes, Donald W. Straughan, James D. Yager, Joanne Zurlo, Bert F.M. Van Zutphen

Experimentation Collection

This is the report of the eleventh of a series of workshops organised by the European Centre for the Validation of Alternative Methods (ECVAM), which was established in 1991 by the European Commission. ECVAM's main goal, as defined in 1993 by its Scientific Advisory Committee, is to promote the scientific and regulatory acceptance of alternative methods which are of importance to the biosciences and which reduce, refine or replace the use of laboratory animals. One of the first priorities set by ECVAM was the implementation of procedures which would enable it to become well-informed about the state-of-the-art of non-animal test …

How The Rat Turned White, Kenneth J. Shapiro

Experimentation Collection

This is the first in a three-part series on the use of animals in psychological research. In it, I describe how animals got into laboratories in the first place, and their purpose and life there. In the second, I will describe animal model research, the strategy whereby psychologists' develop nonhuman animal models to study human psychopathology. In the concluding piece, I will present a critique of this enterprise, using original data I gathered. The three articles are based on a forthcoming book, Animal Models of Human Psychology: Science, Ethics, and Policy.

Sex And Temperament In Modern Society: A Darwinian View Of The “Glass Ceiling” And The “Gender Gap” In Compensation, Kingsley R. Browne

Law Faculty Research Publications

No abstract provided.

Sharing Information Benefits The Meat Industry, Michael Paton

Journal of the Department of Agriculture, Western Australia, Series 4

Cronic health problems of livestock, seldom detected on farms, reduce the efficiency of abattoirs and returns to producers. But a major project providing information to producers from abattoirs is indicating how everyone could benefit from feedback.

Pesticide Safety: A Photonovel, M. Susan Jones, The Kentucky Partnership For Farm Family Health & Safety

Nursing Faculty Publications

No abstract provided.

The Animal Research Controversy: Protest, Process & Public Policy, Andrew N. Rowan, Franklin M. Loew, Joan C. Weer

eBooks

The controversy today regarding the use of animals in research appears on the surface to be a strongly polarized struggle between the scientific community and the animal protection movement. However, there is a wide range of opinions and philosophies on both sides. Mistrust between the factions has blossomed while communication has withered. Through the 1960s, 1970s and early 1980s, the animal movement grew in numbers and financial resources, and developed much greater public recognition and political clout. The research community paid relatively little attention to the animal movement for much of this period but, alarmed by several public relations coups …

State Of Maine Handbook For Hazardous Waste Generators, Department Of Environmental Protection

Maine Collection

State of Maine Handbook for Hazardous Waste Generators

Department of Environmental Protection, Augusta, Maine 1995.

Contents: Introduction to Hazardous Waste / Purpose of This Handbook / Who Generates Hazardous Waste and How? / How Do I Determine If I Generate Hazardous Waste? / Am I a Small Quantity Generator (SQG), an SQG Plus, or a Generator? / Hazardous Waste Management Practices / Manifesting Hazardous Waste / In Summary / Appendix A - D

Information Theoretic Mask Analysis Of Rainfall Time Series Data, Martin Zwick, Hui Shu, Roy Koch

Complex Systems Faculty Publications and Presentations

This study explores an information-theoretic/log-linear approach to multivariate time series analysis. The method is applied to daily rainfall data(4 sites, 9 years), originally quantitative but here treated as dichotomous. The analysis ascertains which lagged variables are most predictive of future rainfall and how season can be optimally defined as an auxiliary predicting parameter. Call the rainfall variables at the four sites A...D, and collectively, Z, the lagged site variables at t-1, E,,,H, at t-2, I...L, etc. and the seasonal parameter, S. The best model, reducing the Shannon uncertainty, u(Z), by 22%. is HGFSJK Z, where the independent variables, H through …

Media Components Influence Viral Gene Expression Assays In Human Fetal Astrocyte Cultures, Micheline Mccarthy, Charles Wood, Larisa Fedoseyeva, Scott R. Whittemore

Nebraska Center for Virology: Faculty Publications

In vitro neurovirological studies of viral infectivity or viral gene expression may be confounded by the mulHple neural cell types and/or fibrob last contamination present in early passage cultures prepared from dissociated human central nervous system (eNS) tissue. We have developed highly enriched astrocyte cultures for neurovirological study by culturing in a serum-free defined medium, 816, supplemented with basic fibroblast growth factor (FGF-2). Subculture in this medium selects against fibroblast proliferation and favors sustained proliferation of a highly enriched glial fibrillary acidic protein (GFAP)-positive cell population. These astrocytes support productive replication of cytomegalovirus (CMV) and transient expression of transfected CMVand …

Seroprevalence Of Toxoplasma Gondii In Domestic Animals In Pakistan, M Zaki

Department of Pathology and Laboratory Medicine

The seroprevalence of Toxoplasma gondii infections among food animals in South-West Pakistan was measured by serological examination using a commercial latex agglutination test (LAT). In all 262 blood samples, from 100 cattle, 40 sheep, 58 goats and 64 chickens, were collected from the city abattoir. Titers considered diagnostically significant (> or = 1:64) were detected in 25% of cattle and 2.5% of sheep. None of the goats and chickens presented seropositivity for anti-toxoplasma antibodies. The study suggests the need for further investigations to determine the impact of these findings on the human population.

Cellular Mechanisms Underlying Myogenic Reactivity In Isolated Arterioles, Hui Zou

Theses and Dissertations in Biomedical Sciences

The myogenic reactivity provides one of the principal mechanisms for blood flow autoregulation. The aims of the performed studies described in this dissertation were to test the role of [Ca²⁺]_i and MLC phosphorylation in arteriolar myogenic reactivity and further examine the source(s) of activator Ca²⁺ required to initiate and maintain myogenic vasoconstriction. In addition, the possible underlying mechanism of contractile protein expression was also addressed.

These studies used male Sprague Dawley rats of 200 ~ 350 grams body weight. Experiments were carried out using rat cremaster first order arterioles and mesenteric vessels. Gel electrophoresis and immunoblotting …

Is There A Difference In Cortical Representation Between Dominant And Non-Dominant Arm Muscles Of Elite Badminton Players?, Dylan J. Edwards

Theses : Honours

Training for sport involves the development of skill and coordination. The physiological changes associated with skill acquisition are complex and at present poorly understood. One of the areas in the central nervous system thought to be involved with skill acquisition is the cerebral motor cortex where localised areas are responsible for controlling specific muscle groups. Learning or improving a motor skill may require reorganisation of the cortical areas controlling relevant muscles to accommodate the new skill. To test this idea we studied a group of elite badminton players that were highly skilled in their dominant playing arm. Transcranial magnetic stimulation …

Effect Of Exercise Induced Muscle Soreness On The Motor Control Properties Of The Biceps Brachii, Alan J. Pearce

Theses : Honours

The objective of this study was to note the time course changes for up to 28 days on the motor control properties of biceps brachii muscle following a bout of eccentric exercise. Eight subjects (5 male, 25-40 years of age) performed 35 maximal voluntary eccentric contractions with the non-preferred arm of the elbow flexors through 130° of extension of 90°s^-1. Voluntary electromyographic (EMG) activity and motor evoked potentials (MEPs) elicited by transcranial magnetic stimulation (TMS) were recorded via surface electrodes placed over the belly of the biceps brachii muscle. Maximal isometric strength was measured at 90° elbow flexion. …

Localisation Of The Gene For A Novel Form Of Charcot-Marie-Tooth Disease In An Isolated Population, Kaite Honeyman

Theses : Honours

Localising the gene for a previously undescribed autosomal recessive form of CMT involved the use of a relatively new approach to rapid genome screening based on the identification of segments which are inherited identical by descent (IBD) from common founding ancestors. It is most feasible for populations which have been founded relatively recently (say less than 25 generations) and which have remained relatively isolated either geographically or culturally. The method is not suitable for highly inbred populations, that is with first and second cousin matings, as many segments will be inherited by chance. It appears to be a suitable screening …

Certain Canine Weakly Β-Hemolytic Intestinal Spirochetes Are Phenotypically And Genotypically Related To Spirochetes Associated With Human And Porcine Intestinal Spirochetosis, Gerald E. Duhamel, Nagaraja Muniappa, Michelle R. Mathiesen, J. L. Johnson, J. Toth, R. O. Elder, A. R. Doster

School of Veterinary and Biomedical Sciences: Faculty Publications

Four canine weakly β-hemolytic intestinal spirochetes associated with intestinal spirochetosis (IS-associated WBHIS) were compared with IS-associated human and porcine WBHIS and the type species for Serpulina hyodysenteriae and S. innocens by using phenotypic and genotypic parameters. The IS-associated canine, human, and porcine WBHIS belonged to a phyletic group distinct from but related to previously described Serpulina type species.

Cluster Analysis Of The Coprolites From Antelope House: Implications For Anasazi Diet And Cuisine, Mark Q. Sutton, Karl Reinhard

Karl Reinhard Publications

This paper reports on a cluster analysis of 155 coprolites from Antelope House, a prehistoric Anasazi site in Canyon de Chelly, Arizona. The analysis revealed three primary clusters; whole kernel maize, milled maize, and nonmaize, which we believe to represent seasonal- and preference-related cuisine. Protein residue analysis on a subsample of the specimens added depth to the analysis.

The Relationship Among Isokinetic Knee Parameters And Three Functional Tests, Kristen Brinks, Ronda Delong, Tanya Stout

Masters Theses

The purpose of this study was to investigate the correlation between knee strength and endurance, as tested by the Biodex{dollar}\sp\circler{dollar} isokinetic system, and the lateral step-up, cross-over hop for distance, and triple hop for distance. Subjects included 20 males and 30 females aged 21-40 years with no history of low back, hip, knee, or ankle injury that was treated by a physician. Bilateral knee flexion and extension strength, power, and endurance was measured at 60, 180 and 300 degrees/second with the hip extended and flexed 115{dollar}\sp\circ{dollar}. Isokinetic data were normalized to body weight and correlated to hop distance which was …

The Effect Of Family Participation On The Outcome Of Patients With Traumatic Brain Injury In Rehabilitation, Grant Middleton, Brett Ransom

Masters Theses

The purpose of this study was to explore the relationship between physical therapists' perceptions and institutional policies relating to family participation in rehabilitation and rehabilitation outcome of patients with traumatic brain injury. Utilizing surveys, we collected information about institutional policy from institutions that were members of the Commission for Accreditation of Rehabilitation Facilities (CARF) in the Midwestern United States. Thirteen institutional surveys provided usable Functional Independence Measure (FIM) scores for patients rehabilitated in 1993, which were used as the outcome measure. The therapists' perceptions survey (n = 104) gathered data about the belief of physical therapists relating to family involvement …

Phase Ii Trial Of Murine Monoclonal Antibody D612 Combined With Recombinant Human Monocyte Colony-Stimulating Factor (Rhm-Csf) In Patients With Metastatic Gastrointestinal Cancer, Mansoor Saleh, Michael Khazaeli, Richard Wheeler, Pat Bucy, Tiepe Liu, Michael Everson, David Mun, Jeff Schlom

Haematology and Oncology, East Africa

In a Phase II study, 14 patients with metastatic gastrointestinal cancer received the mAb D612 (40 mg/m2, days 4, 7, and 11) in combination with recombinant human monocyte colony-stimulating factor [(rhM-CSF) 80 ug/kg/days 1-14]. The combined treatment was well tolerated and resulted in characteristic biological activity associated with each of the agents. Thus, 10 of 14 patients experienced D612-associated secretory diarrhea, which responded to the prostaglandin inhibitor Indomethacin in 5 of 7 patients. rhM-CSF therapy was associated with peripheral mono- cytosis (peak absolute monocyte count, 1444 ±394/mm3) and thrombocytopenia (nadir count, 78 ±10/nim '). Monocyte surface marker analysis revealed a …

Cd16+ Monocytes In Patients With Cancer: Spontaneous Elevation And Pharmacologic Induction By Recombinant Human Macrophage Colony- Stimulating Factor, Mansoor Saleh, Goldman Samuel, Lobuglio Albert, Beall Arthur, Sabio Hernan, Mccord Melissa, Minasian Lori, Alpaugh Katherine, Weiner Louis, Munn David

Haematology and Oncology, East Africa

The small subset of circulating monocytes that express the maturation- associated CD16 antigen has recently been reported to be elevated in patients with bacterial sepsis. We now show that this novel CD16+ monocyte population is also spontaneously expanded in patients with cancer. We studied 14 patients with metastatic gastrointestinal carcinoma enrolled in a clinical trial of recombinant human macrophage colony-stimulating factor (rhMCSF) plus monoclonal antibody D612. We found that before any cytokine treatment, 12 of 14 patients constitutively displayed significant elevations in both the percentage and the absolute number of CD16+ monocytes, as compared with both normal subjects and ill …

In Vitro Effects Of Two Silver Electrodes On Select Wound Pathogens, Linda Jean Laatsch-Lybeck, Poh Chye Ong, Luther C. Kloth

Physical Therapy Faculty Research and Publications

The use of electrical current to promote wound healing is well documented. However, little is understood about the effects of micro-amperage direct current (μADC) on growth of wound pathogens. The purpose of this project was to investigate the antibacterial effects of a silver wire/silver nylon electrode carrying μADC in vitro on several Gram positive and Gram negative bacteria. The current was delivered via silver wire and silver nylon electrodes at an amplitude of 100 μA for a 30-minute duration in an in vitro system. Results demonstrated that only silver wire carrying current inhibited bacterial growth around …

Normal Kinetic Patterns Of The Lower Extremities During Natural Walking In Children Aged Six To Ten, Thomas Ellexson, Christopher Nawrocki, Darrin Schober

Masters Theses

The purpose of this research was to initiate a normative pediatric three-dimensional lower extremity kinetic database specific to the Mary Free Bed Hospital/Grand Valley State University Center for Human Kinetic Studies. Twenty healthy children (eleven girls and nine boys) with a mean age of 7.9 years volunteered for this study. Lower extremity kinetic data were obtained using a computerized three-dimensional motion analysis system coupled with a force plate. Kinematic and kinetic data were pooled and mean/standard deviations at each one percent of the gait cycle were determined for the twenty normative children. Results of the pediatric kinetic data were similar …

The Effects Of Partial Unweighting On Hemiplegic Gait, Natasha A. Creaser, Michael W. Jones

Masters Theses

This study investigated the effects of 30% body weight support on gait in two individuals with hemiplegia secondary to stroke. Two subjects with right hemiplegia were tested. Each completed four trials, two full weight bearing and two unweighted 30%, in random order. Each trial consisted of walking six meters on a treadmill at the subjects' self-selected speed. Temporal distance data were collected via Stride Analyzer footswitches. Subjects were also videotaped from the side during all trials. We expected velocity, cadence, stride length, uninvolved limb swing time, and involved limb stance time to increase with unweighting. Subjects 1's results agreed only …

Daughter Cells Of Saccharomyces Cerevisiae From Old Mothers Display A Reduced Life Span, Nicanor Austriaco, Brian K. Kennedy, Leonard Guarente

Biology Faculty Publications

The yeast Saccharomyces cerevisiae typically divides asymmetrically to give a large mother cell and a smaller daughter cell. As mother cells become old, they enlarge and produce daughter cells that are larger than daughters derived from young mother cells. We found that occasional daughter cells were indistinguishable in size from their mothers, giving rise to a symmetric division. The frequency of symmetric divisions became greater as mother cells aged and reached a maximum occurrence of 30% in mothers undergoing their last cell division. Symmetric divisions occurred similarly in rad9 and ste12 mutants. Strikingly, daughters from old mothers, whether they arose …

Ua66/14/4 Kentucky Partnership Newsletter, Vol. Ii, No. 6, Kentucky Partnership For Farm Family Health & Safety

University Organizations

Newsletter created by and about the Kentucky Partnership for Farm Family Health & Safety.