Medicine and Health Sciences Commons^™

Acyclic Phosphonomethylether Nucleosideinhibitors Of Respiratory Viruses, Dale L. Barnard, N. Bischofberger, C. U. Kim, J. H. Huffman, R. W. Sidwell, J. P. Dougherty, W. Lew, M. A. Williams, W. Yang

Animal, Dairy, and Veterinary Science Faculty Publications

A series of acyclic phosphonomethylether nucleosides were synthesized and then evaluated for inhibitory activity against respiratory viruses of clinical significance using CPE inhibition, neutral red uptake and virus yield reduction assays. Of the 20 compounds synthesized, none significantly inhibited influenza A or B viruses or respiratory syncytial virus strains A2, Long or 18537; the selective indices (SI) were less than 10. A new compound, GS-2128 (2R, 5R-9-[2,5-dihydro-5-(phosphonomethoxy)-2-furanyl]adenine; D4API), selectively inhibited adenovirus 5 (SI>10) as did GS-0577 (9-(3-hydroxy-2-phosphonylmethoxypropyl)-adenine; HPMPA) and GS-0504 [(S)-1-[3-hydroxy-2-(phosphonylmethoxypropyl)]-cytosine; HPMPC]. The 50% effective concentrations (EC50) ranged from 8-100 μg mL-1 and 50% cell inhibitory concentrations (CC50) from …

Choline Acetyltransferase And Carnitine Acetyltransferase Activity In Human Spermatozoa During Capacitation, Lisa A. Eccles

Theses and Dissertations in Biomedical Sciences

The regional distribution of ChAT activity in human spermatozoa is altered during in vitro capacitation and it correlates with the fertilizing potential of sperm.

Regional immunoreactivity in human spermatozoa as assessed by fluorescent immunocytochemistry was compared with ChAT and CaAT activity determined by enzymatic methodologies. Increasing proportions of sperm exhibited ChAT immunoreactivity along the equatorial region with a concomitant decrease in ChAT reactivity in the midpiece. Also, competitive studies with unlabeled ChAT blocked the equatorial region labeling; the unlabeled CaAT blocked staining along the midpiece region of the tail, suggesting some cross-reactivity of the ChAT antiserum with the CaAT enzyme. …

Arterial Wall Hypertrophy And Pdgf-A Expression In One-Kidney, One-Clip Hypertension And Angiotensin Ii Infusion Are Mediated By Elevated Arterial Pressure, Sheri Blair Parker

Theses and Dissertations in Biomedical Sciences

These studies were designed to characterize the relative roles of angiotensin II (ANG II) and pressure in the structural alterations that occur in experimental hypertension. Two separate studies were performed in order to differentiate these two mechanisms. First, male Wistar rats were subjected to one-kidney, one-clip (1K1C) hypertension or sham operation. Losartan, an AT1 receptor antagonist, was administered chronically to half of each surgical group beginning one day before the operation and continued until the end of the study. In a second experiment, rats were implanted with osmopumps delivering either ANG II or saline. To maintain pressure in the normotensive …

Competitive Orientations And Motives Of Adult Sport And Exercise Participants, Diane L. Gill, Lavon Williams, Deborah A. Dowd, Christina M. Beaudoin, Jeffrey J. Martin

Kinesiology, Health and Sport Studies

Participants in four different adult sport and exercise programs (running club, exercise classes, cardiac rehabilitation program, senior games) completed measures of competitive orientation and participation motivation. Our samples were older and more diverse than samples of previous research, and their competitive orientations and motives were similarly diverse. Multivariate analyses revealed gender and sample differences. Males were higher than females on competitiveness and win orientation, and runners were less win-oriented than other groups, but overall, all groups were similar to previous college-age samples in competitive orientation. Groups varied on specific motives, with females rating fitness, flexibility, affiliation, and appearance higher than …

An Analysis Of Two Tests For Diesel Contamination In The Environment When Considering The Impact On Hydrocarbon Degrading Bacteria, Chris Wedding

Mahurin Honors College Capstone Experience/Thesis Projects

No abstract provided.

Gross And Histological Features Of A Myofascial Trigger Point In The Upper Trapezius, Kathryn E. Levee

Electronic Theses and Dissertations

The purpose of this study was to precisely locate, in living humans, a myofascial trigger point associated with the upper portion of the trapezius muscle (TrP1) that refers pain to the head and neck and to determine if this point is associated with anatomical structures. This study is descriptive and utilizes data from measurements of the location of TrP1 in relation to anatomical landmarks, of pressure sensitivity overlying the trigger point and electromyography recordings in localizing the trigger point. Information obtained from living humans was used to determine anatomical correlation to structures in cadavers. Results indicated there is little variability …

Helminths Of The Southwestern Toad, Bufo Microscaphus, Woodhouse's Toad, Bufo Woodhousii (Bufonidae), And Their Hybrids From Central Arizona, Stephen R. Goldberg, Charles R. Bursey, Keith B. Malmos, Brian K. Sullivan, Hay Cheam

Great Basin Naturalist

The gastrointestinal tracts, lungs, and urinary bladders from 77 Bufo microscaphus, 61 Bufo woodhousii, and 8 of their hybrids were examined for helminths. One species of trematode (Glypthelmins quieta), 1 species of cestode (Distoichometra bufonis), and 5 species of nematodes (Aplectana incerta, A. itzocanensis, Rhabdias americanus, Physaloptera sp., and Physocephalus sp.) were found. The greatest prevalence (41%) and mean intensity (231.7) were recorded for Aplectana incerta in Bufo woodhousii. It appears hybrids harbor fewer parasites than either parent species.

Cellular Basis Of Decreased Immune Responses To Pneumococcal Vaccines In Aged Mice, Manju Garg, Wei Luo, Alan M. Kaplan, Subbarao Bondada

Microbiology, Immunology, and Molecular Genetics Faculty Publications

Previously, model systems were developed in our laboratory to study murine immune responses to the 23-valent pneumococcal polysaccharide vaccine Pnu-Imune, both in vivo and in vitro (M. Garg and B. Subbarao, Infect. Immun. 60:2329-2336, 1992; M. Garg, A. M. Kaplan, and S. Bondada, J. Immunol. 152: 1589-1596, 1994). Using these systems, we found that aged mice did not respond to the vaccine in vivo or in vitro. Cell separation studies showed that the unresponsiveness of the aged spleen cells to the vaccine was not due to an intrinsic B-cell defect or to T-cell-mediated immunosuppression but resulted from an accessory cell …

Parotid Gland Lipoma: A Rare Entity, S Muzaffar, N Kayani, S H. Hasan

Department of Pathology and Laboratory Medicine

No abstract provided.

The Product Of The Saccharomyces Cerevisiae Rss1 Gene, Identified As A High-Copy Suppressor Of The Rat7-1 Temperature-Sensitive Allele Of The Rat7/Nup159 Nucleoporin, Is Required For Efficient Mrna Export, Veronica Del Priore, Christine A. Snay, Andre Bahr, Charles N. Cole

Dartmouth Scholarship

RAT7/NUP159 was identified previously in a screen for genes whose products are important for nucleocytoplasmic export of poly(A)+ RNA and encodes an essential nucleoporin. We report here the identification of RSS1 (Rat Seven Suppressor) as a high-copy extragenic suppressor of the rat7-1 temperature-sensitive allele. Rss1p encodes a novel essential protein of 538 amino acids, which contains an extended predicted coiled-coil domain and is located both at nuclear pore complexes (NPCs) and in the cytoplasm. RSS1 is the first reported high-copy extragenic suppressor of a mutant nucleoporin. Overexpression of Rss1p partially suppresses the defects in nucleocytoplasmic export of poly(A)+ RNA, rRNA …

Santa Clara Magazine, Volume 38 Number 4, Fall 1996, Santa Clara University

Santa Clara Magazine

4 - ENTER THE CLASS OF 2000 They're committed, they're ambitious, they're adventurous-and they're here! By Christine Courard '97 Photographs by Charles Barry

10 - A COMMUNITY OF SCHOLARS FOR THE COMMON GOOD Unique gifts of students, facu lty, staff, and alumni advance University efforts to integrate learning and living. By Paul Locatelli, S.J, '60

13 - HEADY DAYS, MYLANTA NIGHTS Riding the roller coaster of high tech investing. Coming out on top. By Therese Poletti '81

18 - THE TAO OF PERSONAL LEADERSHIP New book by English Department chair describes how ancient Chinese philosophy can help leaders bring a …

Activation Of The Human Thymidine Kinase (Tk) Promoter By Simian Virus 40 Large T Antigen Requires Both The T Antigen Prb Family-Binding Domain And Tk Promoter Sequences Resembling E2f-Binding Sites., Michelle M. Anderson, Jun Chen, Charles N. Cole, Susan E. Conrad

Dartmouth Scholarship

Infection of quiescent cells with the DNA tumor virus simian virus 40 induces expression of the cellular thymidine kinase (TK) gene a minimum of 10- to 20-fold, and this induction depends upon the viral protein large T antigen (T-Ag). To define both human TK promoter elements and T-Ag functional domains required for transcriptional induction, we have established a system in which stable Rat-1 transfectants harboring TK promoter-luciferase hybrid genes are infected with recombinant adenoviruses expressing either wild-type or mutant forms of T-Ag and luciferase expression is measured as an indicator of promoter activity. The results show that (i) a 135-bp …

Prevalence Of Antibodies To Feline Parvovirus, Calicivirus, Herpesvirus, Coronavirus, And Immunodeficiency Virus And Of Feline Leukemia Virus Antigen And The Interrelationship Of These Viral Infections In Free-Ranging Lions In East Africa, Regina Hofmann-Lehmann, Daniela Fehr, Markus Grob, Muhamed Elgizoli, Craig Packer, Janice S. Martenson, Stephen J. O'Brien, Hans Lutz

Biology Faculty Articles

While viral infections and their impact are well studied in domestic cats, only limited information is available on their occurrence in free-ranging lions. The goals of the present study were (i) to investigate the prevalence of antibodies to feline calicivirus (FCV), herpesvirus (FHV), coronavirus (FCoV), parvovirus (FPV), and immunodeficiency virus (FIV) and of feline leukemia virus (FeLV) antigen in 311 serum samples collected between 1984 and 1991 from lions inhabiting Tanzania’s national parks and (ii) to evaluate the possible biological importance and the interrelationship of these viral infections. Antibodies to FCV, never reported previously in free-ranging lions, were detected in …

Review Of: Science Advice To The President (William T. Golden, Ed. Aaas Press 2d Ed. 1993)., Timothy Van Dyke

RISK: Health, Safety & Environment (1990-2002)

Review of: Science Advice to the President (William T. Golden, ed. AAAS Press 2d Ed. 1993). Acknowledgments, index of names, introductions, notes, tables. ISBN 0-87168-509-4 [340 pp. $29.95 paper. American Association for the Advancement of Science, 1333 H St. NW, Washington DC 20701.]

Human Immunodeficiency Virus (Hiv)/Hepatitis B Virus (Hbv) Risk Perception Related To Needlestick Injuries At A Medical School In Norfolk, Virginia, Ronald B. Hartman

Community & Environmental Health Theses & Dissertations

A limited number of studies have been conducted to assess the perceptions of risk of medical students and residents for acquiring Human Immunodeficiency Virus (HIV) or Hepatitis B Virus (HBV) via percutaneous (needlestick) injuries while performing clinical training. The purpose of this study is to investigate these perceptions of risk, and to determine whether there is an association between a high perception of risk and a low incidence of needlestick injury. The method of investigation was a four-page survey questionnaire. The instrument was investigated for reliability by use of a pilot study with a small group of medical students and …

An Animal Model For Development Of Pulpal Inflammation, John W. Masters

Loma Linda University Electronic Theses, Dissertations & Projects

Several animal models have been proposed to develop pulpal inflammation. Procedures recommended in these models have many variables and have not produced consistent results. The purpose of this study was to create pulpal inflammation using the Nd:YAG laser.

Forty-five cuspids of 12-18 month old beagle dogs were lased at 5, 7.5, and 10 watts (W) for 20 seconds with a 600 micron contact probe. Five cuspids were not lased but were used as controls. Following laser application, the teeth were extracted at intervals of 3, 7, and 30 days. They were then fixed in 10% formalin, decalcified, sectioned, and stained …

Interleukin-10 Inhibits Tumor Metastasis Through An Nk-Cell Dependent Mechanism, Li-Mou Zheng, David M. Ojcius, F. Garaud, C. Roth, E. Maxwell, Z. Li, H. Rong, J. Chen, X. Y. Wang, J. J. Catino, I. King

All Dugoni School of Dentistry Faculty Articles

Interleukin-10 (IL-10) is a recently described pleiotropic cytokine secreted mainly by type 2 helper T cells. Previous studies have shown that IL-10 suppresses cytokine expression by natural killer (NK) and type 1 T cells, thus down-regulating cell-mediated immunity and stimulating humoral responses. We here report that injected IL-10 protein is an efficient inhibitor of tumor metastasis in experimental (B16-F10) and spontaneous (M27 and Lox human melanoma) metastasis models in vivo at doses that do not have toxic effects on normal or cancer cells. Histological characterization after IL-10 treatment confirmed the absence of CD8+ and CD4+ T cells and macrophages at …

Resistance Of Blastocystis Hominis Cysts To Chlorine, M Zaki, V Zaman, N A. Sheikh

Department of Pathology and Laboratory Medicine

No abstract provided.

Santa Clara Magazine, Volume 38 Number 3, Summer 1996, Santa Clara University

Santa Clara Magazine

6 - THE BEATEN TRACK SCU Psychologist Stephanie Etukudo and Senior Lecturer in English Jeffrey Zorn debate whether ability groupings serve students' best interests.

12 - WHAT WOULD SOLOMON SAY? Adoptions gone awry trigger controversy over how to safeguard the rights of parents while protecting the welfare of the child. By Elizabeth Fernandez '79

18 - A THIRST FOR HEALING A pilgrim to Lourdes finds that the cures offered by the blessed waters can be mostly invisible. By Dee Danna '68 (MBA '72)

24 - THE FLOWERING OF SANTA CLARA UNIVERSITY For more than a century, the Mission Gardens have …

Subcutaneous Phaeohyphomycosis: Infection With Pigmented Fungi, I N. Soomro, A Kennedy

Department of Pathology and Laboratory Medicine

No abstract provided.

Neuronal Toxicity Of Humoral Factors From Patients With Diabetic Neuropathy And Mechanism Of Neuronal Death On Cultured Adrenergic Neurons: Studies Of Autoimmune Pathogenesis In Diabetic Neuropathy, Dong Liu

Theses and Dissertations in Biomedical Sciences

Diabetic neuropathy is an extremely common medical problem because of the high frequency of diabetes and its complications. Diabetic neuropathy consists of not one, but many patterns of nerve injury, each potentially with its own discrete pathogenic mechanisms. Evidence is accumulating that indicates autoimmunity plays a role in diabetic peripheral neuropathy. However, the significance of immunopathogenesis of diabetic neuropathy is as yet unclear.

Using the N1E-115 mouse neuroblastoma cell line (NB) as a model of the adrenergic autonomic neuron, we found that sera of IDDM patients with neuropathy inhibit growth and differentiation of adrenergic neurons in culture (Pittenger et al, …

Lead Activation Of A Developmentally Regulated Calcium Channel In Rat Hippocampal Nerve Terminals, Troy E. Rhodes

Theses and Dissertations in Biomedical Sciences

Low level lead (Pb²⁺) exposure may produce lasting deficits in learning and memory by altering calcium (Ca²⁺) dependent processes. Isolated presynaptic nerve terminals from rat hippocampus were loaded with the intracellular (Ca²⁺) indicator Fura-2. The changes in cytoplasmic free calcium ([Ca²⁺]_i) were measured by stopped-flow fluorescence spectroscopy following depolarization with elevated potassium on a millisecond time scale (Lentzner et al., 1992). Depolarization promoted a rapid increase in Ca²⁺_i which occured in two kinetically distinguishable phases: a fast component, representing the activity of rapidly inactivating Ca²⁺ channels (τ …

Genome Research And Traditional Intellectual Property Protection -- A Bad Fit?, Kate H. Murashige

RISK: Health, Safety & Environment (1990-2002)

Dr. Murashige addresses the need for a patent system more closely tailored to the needs of biotechnology. For example, the obviousness requirement may interfere with using patents to recoup high costs of work when it could arguably be done by researchers of ordinary skill.

Impact Of The Human Genome Project At The Interface Between Patent And Fda Laws, Brian C. Cunningham

RISK: Health, Safety & Environment (1990-2002)

Mr. Cunningham stresses the broad scope of biotechnological innovations. Besides endorsing the need for a new oversight commission to deal with potential social issues, he suggests, for example, that some products should be treated like biologics rather than new drugs.

Development Of Vaccines To Meet Public Health Needs: Incentives And Obstacles, Phillip K. Russell

RISK: Health, Safety & Environment (1990-2002)

Dr. Russell explains how such matters as high 'costs of regulation, lack of an effective plan for delivery (particularly abroad) and politics can interfere with providing globally needed vaccines.

Anion Induced Blue To Purple Transition In Bacteriorhodopsin, Mrunalini Pattarkine, Anil K. Singh

Faculty Works

Purple membrane (PM, λ" role="presentation">λmax" role="presentation">max 570 nm) of H. halobium on treatment with sulphuric acid changes its colour to blue (λ" role="presentation">λmax" role="presentation">max 608 nm). The purple chromophore can be regenerated from the blue chromophore by exogeneous addition of anions such as CI−" role="presentation">− and HPO42−" role="presentation">2−4. Chloride ion is found to be more effective than the dibasic phosphate ion in regenerating the purple chromophore. Nevertheless, one thing common to the anion regeneration is that both CI−" role="presentation">− and HPO42−" role="presentation">2−4 show marked pH effect. At pH 1.0 the efficiency of …

Rust Diseases Of Apple, John R. Hartman

Agriculture and Natural Resources Publications

Three related rust diseases occur on apple trees in Kentucky: cedar-apple rust, cedar-hawthorn rust, and cedarquince rust. Crabapple, hawthorn, mountain ash, pear, and serviceberry are also susceptible to these diseases. All three rusts are caused by different species of the fungus Gymnosporangium, each of which must spend a phase of its life cycle as a parasite on Juniperus species such as native red cedars or ornamental junipers.

Although cedar rusts can cause unsightly growths on Juniperus, they do not usually cause serious damage to these plants. Rust diseases can cause serious losses on apples, however, as a result …

Metal-Resistance Genetically Engineered Bacteria, Sylvia Daunert, Donna Scott, Sridhar Ramanathan

KWRRI Research Reports

Bacterial-based electrochemical and optical sensing systems that respond in a highly selective and sensitive manner to antimonite and arsenite have been developed. This was accomplished by using genetically engineered bacteria bearing one of two plasmids constructed for our studies. The first plasmid, pBGD23, contains the operator/promoter region (O/P) and the gene of the ArsR protein from the ars operon upstream from the β-galactosidase gene. In the absence of antimonite/arsenite, ArsR binds to the 0/P site and prevents the transcription of the genes for ArsR and β-galactosidase, thus blocking expression of these proteins. When antimonite or arsenite is present in the …

Periodontal Repair In Dogs : Effect Of Recombinant Human Transforming Growth Factor Beta-1 On Guided Tissue Regeneration, Saghi Shayan Razi

Loma Linda University Electronic Theses, Dissertations & Projects

The objective of this study was to evaluate bone and cementum regeneration in the critical size, supraalveolar, periodontal defect model following surgical implantation of recombinant human transforming growth factor beta-1 (rhTGFß-1) in conjunction with guided tissue regeneration (GTR). Five male beagle dogs were used in a split-mouth design. Alternate jaw quadrants in consecutive animals received GTR with rhTGFß-1 in a resorbable calcium carbonate carrier. Contralateral jaw quadrants received GTR with carrier only (control). The healing interval was 4 weeks. Clinical healing was generally uneventful, however, minor membrane exposures were observed for both treatments with limited gingival inflammation. All defects with …

In Vitro Plaque Formation On Orthodontic Resin Sealants, Leland W. Pack

Loma Linda University Electronic Theses, Dissertations & Projects

The purpose of this study was to examine in vitro plaque formation on sealant coated enamel. The plaque growth on different types of sealants placed on enamel was compared to each other and to an unsealed enamel control. The antimicrobial properties of sealants were also studied.

Sealant coated bovine enamel disks were immersed in a S. mutans culture medium for four days. The amount of bacterial plaque formation was quantified by removing the deposit, suspending it in solution with subsequent serial dilution. Aliquots of the dilutions were then spread on blood agar plates and incubated for 48 hours. The number …