Medicine and Health Sciences Commons^™

Golf Cart Use And Individuals With Disabilities - Will The Pga Tour Ask The Supreme Court?, Joseph H. Huber

Movement Arts, Health Promotion and Leisure Studies Faculty Publications

No abstract provided.

How Has The Supreme Court Affected The Americans With Disabilities Act? You Be The Judge, Joseph H. Huber

Movement Arts, Health Promotion and Leisure Studies Faculty Publications

No abstract provided.

The Difference In Pain Tolerance Between Ncaa Division 1-A Male And Female Athletes, Mollie K. Karabatsos

Theses, Dissertations and Capstones

How well an athlete tolerates pain may aid the athletic trainer in determining a proper rehabilitation protocol for the quickest recovery and return to play after an injury. The present investigation was conducted to determine if there is a difference in pain tolerance between NCAA Division I-A male and female athletes. The subjects consisted of nineteen healthy Division I-A male athletes and nineteen healthy Division I-A female athletes all between the ages of 18 and 23 (N=38). The subjects placed their hand, up to their styloid process, into a one - degree Celsius ice bath for one minute. After one …

Whole Animal And Cellular Strategies In The Regulation Of Bone Mass In The Rat, Weidong Geng

Theses, Dissertations and Capstones

In order to determine the strategies in regulation of bone mass in the rat and better understand the pathophysiological mechanism of the postmenopausal osteoporosis, a series of studies were conducted at the whole animal and cellular level. At the whole animal level, female Sprague-Dawley rats were ovariectomized (Ovx) or sham-operated (control) at 18 weeks and the entire skeleton obtained at 24 weeks (baseline) or after an additional 31 day (28 week) interval on a normal (1.0%) or deficient (0.02%) calcium diet. Ovx rats on the normal calcium diet showed a 42% increase in whole body bone resorption and a 70% …

N-Methyl-D-Aspartate Receptor Subunit Nr2b Is Widely Expressed Throughout The Rat Diencephalon: An Immunohistochemical Study, Arshad Khan, B. Stanley, Lisa Bozzetti, Christina Chin, Cyndi Stivers, Margarita Curras-Collazo

Selected Works Temporary Series

No abstract provided.

The Skn7 Response Regulator Of Saccharomyces Cerevisiae Interacts With Hsf1 In Vivo And Is Required For The Induction Of Heat Shock Genes By Oxidative Stress, Desmond C. Raitt, Anthony L. Johnson, Alexander M. Erkine, Kozo Makino, Brian Morgan, David S. Gross, Leland H. Johnston

Scholarship and Professional Work – COPHS

The Skn7 response regulator has previously been shown to play a role in the induction of stress-responsive genes in yeast, e.g., in the induction of the thioredoxin gene in response to hydrogen peroxide. The yeast Heat Shock Factor, Hsf1, is central to the induction of another set of stress-inducible genes, namely the heat shock genes. These two regulatory trans-activators, Hsf1 and Skn7, share certain structural homologies, particularly in their DNA-binding domains and the presence of adjacent regions of coiled-coil structure, which are known to mediate protein–protein interactions. Here, we provide evidence that Hsf1 and Skn7 interact in vitro and …

Cell Cycle-Dependent Binding Of Yeast Heat Shock Factor To Nucleosomes, Christina Bourgeois Venturi, Alexander M. Erkine, David S. Gross

Scholarship and Professional Work – COPHS

In the nucleus, transcription factors must contend with the presence of chromatin in order to gain access to their cognate regulatory sequences. As most nuclear DNA is assembled into nucleosomes, activators must either invade a stable, preassembled nucleosome or preempt the formation of nucleosomes on newly replicated DNA, which is transiently free of histones. We have investigated the mechanism by which heat shock factor (HSF) binds to target nucleosomal heat shock elements (HSEs), using as our model a dinucleosomal heat shock promoter (hsp82-ΔHSE1). We find that activated HSF cannot bind a stable, sequence-positioned nucleosome in G₁-arrested …

N-Methyl-D-Aspartate Receptor Subunit Nr2b Is Widely Expressed Throughout The Rat Diencephalon: An Immunohistochemical Study, Khan, B. Stanley, Lisa Bozzetti, Christina Chin, Cyndi Stivers, Margarita Curras-Collazo

Departmental Papers (Biology)

No abstract provided.

Lymphocyte Profiles In Blood And Tumors Of Arkansas Rous Sarcoma Regressor And Progressor Chickens, Brant Ward

Inquiry: The University of Arkansas Undergraduate Research Journal

The Arkansas Rous Sarcoma Regressor (AR) and Progressor (AP) lines of chickens represent an excellent model to study immune responses to tumors. Both lines of chickens initially develop tumors when injected with Rous sarcoma viros (RSV) or with DNA coding for the RSV oncogene v-src (v-src DNA). AR chickens will eventually regress the tumors whereas AP chickens will allow the tumors to progress to a terminal stage. By using both v-src DNA and RSV for tumor induction, we were able to compare the immune response to tumor antigens alone and tumor antigens in combination with viral components, respectively. To study …

Fish Health For Fish Farmers - 2nd Edition, Tina Thorne

Fisheries Published Reports

2nd edition.

Disease is a departure from the normal state of health of a fish or any other animal. Diseases have no doubt occurred among fishes since these animals have occupied a place on the earth. Scientific efforts to understand the causes of disease in fishes, and to attempt to control them however, have only been made during the last sixty years.

Fishes, crustaceans and molluscs, as with other animals are subject to a wide spectrum of diseases, that can be classified as infectious or non-infectious. The vast majority of disease problems in the intensive farming of aquatic animals can …

Response To Critique Of The Claim Of Cannibalism At Cowboy Wash, Patricia M. Lambert, Banks L. Leonard, Brian R. Billman, Richard A. Marlar, Margaret E. Newman, Karl J. Reinhard

Karl Reinhard Publications

The original authors of Billman et af. (2000) are joined by three other analysts from the Cowboy Wash research team to respond to the critique of this article by Dongoske et af. (2000). Dongoske and his coauthors state that Billman et af. (2000) failed to test alternative hypotheses or to consider alternative explanations for the findings at 5MTJOOJO and similar sites. The original authors point out that alternative hypotheses were examined and rejected, leaving a violent episode of cannibalism as the most plausible explanation for the remains found at 5MTJOOJO. Dongoske et af. also question many aspects of the osteological, …

Expression Of Biologically Active Human Butyrylcholinesterase In The Cabbage Looper (Trichoplusia Ni), Peter L. Platteborze, Clarence A. Broomfield

United States Army: Publications

This investigation examined the utility of three recombinant protein-expression systems (COS cells, insect cells and insect larvae) to cost-effectively produce biologically active human butyrylcholinesterase (BuChE). It was determined that baculovirus-infected insect cells (Sf9 and High 5) expressed 3.5- and 8.2-fold, respectively, more active enzyme than COS-7 cells. Baculovirus-infected cabbage looper (Trichoplusia ni) insect larvae produced over 26 times more than High 5 cells ; in fact, one baculovirus-infected insect larva provided more active protein than 100 ml of insect cell culture. Analysis of the larvally expressed proteins revealed that the vast majority of BuChE expressed was inactive due to …

History, Religion And Meaning: American Reflections On The Holocuast And Israel, Jules Simon

Departmental Papers (Philosophy)

No abstract provided.

A Simple Method For Generating Full Length Cdna From Low Abundance Partial Genomic Clones, Yongxin Wang, Joseph M. Fugaro, Fauzia Siddiq, Chandra Mouli V. Goparaju, Fulvio Lonardo, Anil Wali, John F. Lechner, Harvey I. Pass

Wayne State University Associated BioMed Central Scholarship

Abstract

Background

PCR amplification of target molecules involves sequence specific primers that flank the region to be amplified. While this technique is generally routine, its applicability may not be sufficient to generate a desired target molecule from two separate regions involving intron /exon boundaries. For these situations, the generation of full-length complementary DNAs from two partial genomic clones becomes necessary for the family of low abundance genes.

Results

The first approach we used for the isolation of full-length cDNA from two known genomic clones of Hox genes was based on fusion PCR. Here we describe a simple and efficient method …

Method Development And Validation For Drug Analysis By Hplc Gc-Ms, Yann Jen Goh

Student Works (2000-2009)

Enalapril and its metabolite Enalaprilat were determined by gas chromatography - mass spectrometry (GC - MS) and high - performance liquid chromatography (HPLC). For HPLC, the detection limit of enalapril and enalaprilat was 10n g/ml in methanol, whereas for the GC - MS, the detection limit was 6.25ng/ml in plasma. These methods were compared and GC - MS was selected for routine analysis. Enalapril and Enalaprilat in plasma were extracted and cleaned up by using Sep - Pak C 18. Enalapril and Enalaprilat were detected after reaction with diazomethane and were identified by gas chromatography - mass spectrometry as methyl …

Gas Chromatographic-Mass Spectrometric Analysis Of Captopril And Its Application In Pharmacokinetic Studies In Healthy Subjects, Zamri Chik

Student Works (2000-2009)

Captopril is a highly specific competitive inhibitor of the angiotensin-convening enzyme (ACE). This enzyme converts angiotensin I, a relatively inactive decapeptide, to angiotensin II , a potent endogenous vasoconstrictor substance. Captopril is widely used for the treatment of hypertension and congestive heart failure. This drug, which contains a sulphydryl group binds readily to albumin and to other plasma proteins. Captopril is unstable in blood and plasma ex vivo because of the reactivity of sulphydryl group which results in oxidation and rapid formation of disulphides. Therefore a fixative or a stabiliser must be added to each blood sample immediately following collection. …

Application Of Ai In Medicine, Tarmizi Amirul Hisyam

Student Works (2000-2009)

From the beginning of human civilization, mankind have been trying to fins solution on how to make life easier. They create, modify and adapt, as they would please their need. From the smallest things in our life to the biggest part of all technologies, has improved our life tremendously. Computer technology nowadays has an important role in education, communication, medicine, and many more. In the field of medicine, we have seen many support systems had been developed and use to help human in their daily lives. Application of AI in medicine was proposed in this reason in mind, as a …

Role Of KAtp Channels In Reduced Antinociceptive Effect Of Morphine In Streptozotocin-Induced Diabetic Mice, Vivek Sood, Ajay Sharma, Manjeet Singh

Pharmacy Faculty Articles and Research

The nociceptive effect was measured using withdrawal latency in tail flick test in mice rendered diabetic by administering streptozotocin (200 mg/kg, i.p.). The antinociceptive effect of morphine (4 and 8 mg/kg, s.c.) and cromakalim, a KATP channel opener, (0.3, 1 and 2 micrograms, i.c.v.) was significantly reduced in diabetic mice. Moreover, co-administration of cromakalim(0.3 microgram) did not alter the reduced antinociceptive effect of morphine(4 mg/kg) in diabetic mice. Spleenectomy in diabetic mice restored the decrease in antinociceptive effect of morphine and cromakalim. Multiple dose treatment with insulin to maintain euglycaemia for 3 days in diabetic mice prevented the decrease in …

Identification Of The Transactivation Domain Of The Transcription Factor Sox-2 And An Associated Co-Activator, Tamara K. Nowling, Lance R. Johnson, Matthew S. Wiebe, Angie Rizzino

Nebraska Center for Virology: Faculty Publications

The importance of interactions between Sox and POU transcription factors in the regulation of gene expression is becoming increasingly apparent. Recently, many examples of the involvement of Sox-POU partnerships in transcription have been discovered, including a partnership between Sox-2 and Oct-3. Little is known about the mechanisms by which these factors modulate transcription. To better understand the molecular interactions involved, we mapped the location of the transactivation do-main of Sox-2. This was done in the context of its interaction with Oct-3, as well as its ability to transactivate as a fusion protein linked to the DNA-binding domain of Gal4. Both …

Quantitative Image Analysis Of Simian Immunodeficiency Virus Replication In Macrophages Coinfected With Mycobacterium Avium Complex, Qingsheng Li, Keith G. Mansfield, Andrew Lackner, Ashley T. Haase

Qingsheng Li Publications

Mycobacterium avium is the most frequent cause of disseminated bacterial infection in patients with human immunodeficiency virus type 1 infection and in rhesus macaques with simian immunodeficiency virus (SIV) infection. This animal model of AIDS was used to test the hypothesis that this frequent association is the result of reciprocal enhancement of replication of both microorganisms. The replication of M. avium and SIV was analyzed in lymphatic tissues obtained from rhesus macaques experimentally inoculated with SIVmac who developed or remained free of overt M. avium infection. In situ hybridization, quantitative image analysis, and staining of M. avium and of macrophages …

Whole Genome Sequencing Of L.Monocytogenes And L.Innocua : A Basis For Comparative Genomics Of The Genus Listeria, Rachel Purcell

Theses

Listeria monocytogenes is a gram-positive, food-borne human and animal pathogen. L.innocua is a non-pathogenic species the most closely related to L. monocytogenes. Comparative genomics between a pathogenic and a closely related non-pathogenic species is a powerful approach for identifying genes or regions on the chromosome, which might be involved in virulence. Therefore whole genome sequencing of these two organisms was undertaken. The strategy used was based on a random approach combined with end-sequencing of a medium insert library for the finishing phase. Based on the available sequence data for these two species an in silico analysis for the presence of …

Modulation Of The Pharmacokinetics And Pharmacodynamics Of Proteins By Polyethylene Glycol Conjugation, Reza Mehvar

Pharmacy Faculty Articles and Research

With the rapid advances in the field of biotechnology during the last decade, many peptides and proteins have been produced and evaluated for therapy of various diseases, including cancer. However, rapid clearance and the possibility of immunogenicity after the in vivo administration of these biotechnology-driven products have impeded their marketing. To circumvent these problems, synthetic and natural polymers such as polyethylene glycol (PEG) and dextrans, respectively, have been covalently attached to proteins, and some of these protein-polymer conjugates have shown promising therapeutic results. The conjugation of proteins with polymers usually causes a reduction in the recognition of the protein by …

The Effects Of Narrowed Area And Additional Feeding On Some Physiological Characteristics Ofhoney Bee (Apis Mellifera L.) Colonies, Ahmet Güler

Turkish Journal of Veterinary & Animal Sciences

The study was conducted at Alata Horticultural Research institute between 1994 and 1996. The aim of the study was to obtain a strong colony by way of supersedure and empty frames, feeding with saccharose syrup and encouraging egg laying of queen bees at the end of summer. According to the results, there were significant differences among treatments in terms of the development of colony population, brood rearing activity and honey yield. Treatments had no significant effect on wax building activity and swarming tendency.

Fattening Performance And Carcass Characteristics Of Awassi Crosses (F1 And B1) With German Blackheaded Mutton And Hampshire Down Male Lambs, Ali̇ Akmaz, Emi̇n Teki̇n, Ramazan Kadak, Mehmet Gürkan

Turkish Journal of Veterinary & Animal Sciences

This study was carried out to investigate the fattening and carcass characteristics of German Blackheaded Mutton (GBM) x Awassi (A) F1, Hampshire Down (HD) x A (F1) and HD x (HD x A) B1 male lambs. Nine GBM x A (F1), eight (HD) x A (F1) and eight HD x (HD x A) B1 male lambs were used for fattening traits, and six of each breed for carcass traits. The lambs were fed, from the beginning at approximately 20 kg of live weights to the finish at 45 kg, alfalfa hay 100 g/head/day and concentrates ad libitum. For statistical analysis, …

Effects Of Vitamins A, E And Selenium On Reproductive Performance And Serum Levels Ofsome Biochemical Parameters In Ewes, And Birth Weight And Survival Rates In Their Lambs, Mehmet Avci, Zi̇ya Karakilçik, Rahmi̇ Kanat

Turkish Journal of Veterinary & Animal Sciences

It has been reported that vitamins A and E and Se affected the reproductive performance and some biochemical parameters in ewes, and the numbers, concentration and motility of spermatozoa in rams. In addition, Se and vitamin E have important functions in the etiology of WMD. Therefore, this study was designed to investigate the effects of vitamin A and E and Se supplementation on reproductive performance and some biochemical parameters in ewes, and birth weight and survival rates of lambs. Four groups were formed (A, B, C, D) and fed with a vitamin A, E and Se supplemented diet as follows. …

The Possibilities Of Developing Dam And Sire Lines Using Akkaraman, Sakız And Kıvırcıksheep Breeds For Lamb Productioni. Fertility In Akkaraman Sheep, Survival Rate And Growth Characteristics Of Sakız Xakkaraman F_1 And Kıvırcık X Akkaraman F_1 Lambs, Hali̇l Akçapinar, Ceyhan Özbeyaz, Necmetti̇n Ünal, Mustafa Avci

Turkish Journal of Veterinary & Animal Sciences

This study was conducted to investigate the possibilities of developing terminal male and female lines for lamb production by the crossing of native breeds, that is, Akkaraman, Sakız (Chios) and Kıvırcık breeds. The Akkaraman sheep is a fattailed breed raised in central Anatolia, while Sakız and Kıvırcık sheep aren non fattailed breeds raised in western and northern-western Anatolia. The Birth rate of Akkaraman x Akkaraman (AA), Sakız x Akkaraman (SA) and Kıvırcık x Akkaraman (KA) breeding groups were generally found as follows: 94.0, 78.6 and 74.0% in 1996; and 93.3, 90.8 and 85.0% in 1997, respectively. The survival rate of …

The Effect Of Amitraz Poisoning On Formation Of Methemoglobin And Hemolytic Anemia In Guinea-Pigs, Meltem Şi̇reli̇, Vedat Sağmanligi̇l, İlksi̇n Pi̇şki̇n, Ayhan Fi̇lazi̇

Turkish Journal of Veterinary & Animal Sciences

This investigation was carried out to determine the effect of amitraz poisoning on the the formation of methemoglobin and osmotic fragility of erytrocytes. in this study, 12 guinea-pigs, weighing 250-300 gr were used. They were equally divided into two groups, control and experimental. The animals in the experimental group were intraperitoneally injected with amitraz at a dose of 1 mg/kg body weight. One hour after amitraz injections, erytrocyte, hematocrit, hemoglobin levels and also percent hemolysis of erytrocytes were measured in the blood samples taken from the control and experimental groups. In the experimental group, the value of hemoglobin was lower …

Acute Phase Effect Of Trichloroethylene Ingestion On Some Biological Markers In Dogs, Recep Aslan, Mehmet Tütüncü, S. Oktay Aslan, Yilmaz Dündar

Turkish Journal of Veterinary & Animal Sciences

Trichloroethylene (TE) is an environmental toxic solvent hazardous to human and domestic animals and well known in the industrial sector. The purpose of this study was to determine whether oral TE plays a role in lipid oxidation and tissue damage. Fourteen dogs were treated with an oral toxic dose of 0.5 ml/kg T.E. The acute changes that occurred in creatine kimase (CK), malondialdehyte (MDA), superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) were investigated in blood samples taken before application and 8, 24, 48, 72 and 168 hours after treatment. SOD and GSH-Px activities decreased, protein, albumin and alcalen phosphate did …

Use Of Elisa, If A, And A Vidin-Biotin Staining For The Diagnosis Of Bovine Genital Campylobacteriosis, Seyyal Ak, Nuri̇ Turan, Kemal Ak, İ. Kamuran İleri̇, Ati̇lla Ilgaz

Turkish Journal of Veterinary & Animal Sciences

Acid glycine extract (AGE) and whole cell (WC) antibodies wer e used in ELISA testing for the detection of Campylobacter fetus subsp. venerealis and subsp. fetus antibodies in the blood sera of 150 female cattle and 100 bulls which wer e one year old or older . It was observed that AGE antibody was mor e specific than WC antibody . A cr oss r eaction was detected between C. fetus subsp. venerealis and subsp. fetus. Thus, it was concluded that for Campylobacter ELISA could only be used as a monitoring test. In dir ect diagnosis of C. fetus with …

Detection Of Leptospira Species By Polymerase Chain Reaction (Pcr) In Urine Of Cattle, Burhan Çeti̇nkaya, Hasan Basri̇ Ertaş, Hasan Öngör, Adi̇le Muz

Turkish Journal of Veterinary & Animal Sciences

This study was carried out to investigate the prevalance of leptospirosis in the urine of cattle slaughtered in three major abattoirs in the east of Turkey. A polymerase chain reaction (PCR) based on a pair of genus-specific primers was used to detect leptospiral DNA in the urine samples of 473 cattle, 284 of which were from Elazig, 112 from Malatya and 77 from Diyarbakir. The detection limit of the method was determined to be approximately five bacteria per ml of urine. In the examination of urine samples, 4.02% (19/473) (95% confidence intervals [Cl] 2.4-6.2) were found to be positive by …