Medicine and Health Sciences Commons^™

Diagnosis Of Lymphoid Leukosis (Ll) By Elisa (Enzyme-Linked Immunosorbent Assay) And Marek's Disease (Md) By If (Immunofluorescence) Test In Chickens, Selda Özbi̇lgi̇n, Ayşi̇n Şen, Mi̇hri̇ban Ülgen, K. Tayfun Çarli, Gürsel Sönmez, Özlem Özmen

Turkish Journal of Veterinary & Animal Sciences

In this study, our aim was to diagnose lymphoid leukosis and Marek's disease, which are neoplastic diseases of chickens, by ELISA and Indirect Immunofluorescence Test (IIF), respectively. Samples (eggs and tissues) were selectively taken from the flocks suspected of having lymphoid leukosis and Marek's disease. The tissues were macroscopically and microscopically examined. Tissues belonging to some flocks showed typical lesions for lymphoid leukosis and Marek's disease. Seven broiler breede flocks and three commercial layer flocks were tested for avian leukosis virus (ALV) group specific antigen (p27) in the albumen. ALV p27 antigen was detected in five out of ten flocks. …

Multiple-Trait Analysis Using Bayesian Methods In Animal Breeding: A Gibbs Sampling Approach, Mehmet Zi̇ya Firat

Turkish Journal of Veterinary & Animal Sciences

Most animal breeding experiments are based on more than one economically important trait measured in each individual. In the statistical analysis of the resulting data, traits recorded in the same individuals are often considered one at a time. Usually we are interested, however, not only in the mode of inheritance of a particular trait but also in its relationships with other traits. Multivariate analyses are required to make inferences about genetic and phenotypic correlations between traits, but point estimates of such parameters can be poor even when breeding data on hundreds of animals are used. Bayesian methods exclude variance matrices …

The Effects Of Thyroid Disorders On Some Haematologic Parameters In Quail, Mukaddes Özcan, Murat Arslan, Erdal Matur, Ülker Çöteli̇oğlu, Eli̇f Ergül

Turkish Journal of Veterinary & Animal Sciences

In this study, the effects of experimentally induced hyperthyroidism and hypothyroidism on the erythrocyte, leucocyte and haematocrit values, the amount of haemoglobin and plasma glucose, cholesterol, triglyceride, creatinine and lactate dehydrogenase (LDH) levels were investigated. A total of 45 4-week-old female Japanese quail (Coturnix coturnix Japonica) were used. The quails were divided into 3 groups as hyperthyroid, hypothyroid and the control group, with 15 quails in each. The birds in the control group were fed with unsupplemented commercial quail diet for 5 weeks. At a rate of 0.4mg/100g, Levatroxin-sodium was added into the diet of the hyperthyroid group and 0.2 …

Cloning Of Bacillus Subtilis Rskk243 Bifunctional Xylanase Gene In E. Coli And B. Subtilis And Enzyme Characterization, Bahri̇ Devri̇m Özcan, Numan Özcan

Turkish Journal of Veterinary & Animal Sciences

A bifunctional gene xylanase of B. subtilis RSKK243 encoding detectable beta(1,4) glucanase (CMCase) activity in addition to a high level of main xylanase activity was cloned and expressed in Escherichia coli with pUC18 plasmid and in various Bacillus subtilis strains with pUB110 vector. For enzymatic analysis and plasmid isolation, the gene was also expressed in xylanase and beta glucanase negative B.subtilis YB886 strain with pUB110 vector. The pUB110 with bifunctional xylanase gene was transferred into B. subtilis RSKK243 for maximizing the enzyme yield, as well as into other RSKK strains to create novel strains with multiple enzyme capacities. Recombinant strains …

Canine Mast Cell Tumors: A Pathological Survey Of Nineteen Cases, Sevi̇l Atalay Vural, Yilmaz Aydin

Turkish Journal of Veterinary & Animal Sciences

In the survey study, a total of 19 canine mast cell tumors were examined with regard to sex, age, breed of dogs, tumor location, and pathological findings in the period of 1960-1999. The majority of tumors occurred in males (78%) and between the ages of 6 and 11 years (67%). The pointer was predisposed to development of tumors (35%). The hindquarters and hind legs were most commonly involved, followed by the extragenital inguinal area and trunk. The tumors clearly had a site preference for the posterior region of the body (79%). The only extracutaneous tumor was found in the nasal …

The Effects Of Enzyme And Probiotic Supplementation To Diets On Broiler Performance, Mustafa Mi̇di̇lli̇, Şaki̇r Doğan Tuncer

Turkish Journal of Veterinary & Animal Sciences

This study was carried out to determine the effect of the supplementation of enzyme and probiotic to broiler feeds based on barley and wheat on broiler performance, dressing percentage, the small intestinal length and weight, liver and abdominal fat weights, intestinal viscosity and the percentage of sticky droppings. In this experiment, 600 one - day - old Hubbard broiler chicks were used. This study was performed on four treatment groups, each containing 150 chicks. One of the groups was the control group and the others were the experimental groups. Each treatment group was divided into 6 replicates of 25 chicks. …

Use Of Sunflower Meal With Enzyme Mixture Substituted For Soybean Meal In Broiler Diets, Fi̇gen Kirkpinar, Hati̇ce Basmacioğlu

Turkish Journal of Veterinary & Animal Sciences

The study was carried out to investigate the effects of sunflower meal with an enzyme mixture containing \alpha-amylase, ß-glucanase, cellulase, lipase and protease substituted for soybean meal in broiler diets on performance, intestinal weight, viscosity and pH, as well as the frequency of sticky droppings and abdominal fat. In the trial, a total of 960 one-day-old Avian broiler chicks were used. The trial lasted 6 weeks. A total of 12 experimental diets were supplied ad libitum. Dietary treatments consisted of 15%, 25%, 35% soybean meal, or sunflower meal or sunflower meal with the enzyme mixture (0.05% and 0.10%). Enzyme supplementation …

Effects Of Copper Sulphate Administration To Pregnant Sheep On Some Mineral Levels In Blood Sera Of Sheep And Lambs, And Birth Weight Of Lambs, İbrahi̇m Çi̇mtay, Teki̇n Şahi̇n, Ali̇ Ölçücü, Gürbüz Aksoy

Turkish Journal of Veterinary & Animal Sciences

In this study, copper sulphate was administered during the last period of pregnancy to sheep in the Şanlıurfa region in which enzootic ataxia was encountered in the past. The objectives of this study were to search the effect of copper sulphate administration on some mineral levels (copper, zinc, iron, calcium and magnesium) in blood serum of pregnant sheep and their lambs, and birth weight of lambs. This study was performed on 35 pregnant sheep (control group: 15, experimental group: 20). In the experimental group, copper sulphate (2% solution) was administered orally at sixth and fourth weeks before birth at 2 …

Survey Of European Foulbrood Disease In Bee Farms Located In Elazığ And The Surrounding Provinces, Hali̇l Şi̇mşek, Canan Özcan

Turkish Journal of Veterinary & Animal Sciences

A total of 250 specimens from 110 bee farms located in Elazığ and the surrounding provinces were taken in 1999 and 2000. The specimens were examined for European Foulbrood Disease. Under macroscopic examination clinical symptoms were noted in 12 specimens, and Paenibacillus alvei was isolated and identified from 8 of these specimens. As a result of the antibiotic sensitivity test, the isolate was found sensitive to enrofloxacin, streptomycin and erythromycin 75%, 75%, and 62,5% of the time respectively. In this study, the presence of European Foulbrood Disease was determined in 3.2% of cases in Elazığ and the surrounding provinces.

Effects Of Copper Sulphate Administration On Body Weight Gain And Some Blood Parameters In Lambs, Teki̇n Şahi̇n, İbrahi̇m Çi̇mtay, Gürbüz Aksoy, Ali̇ Ölçücü

Turkish Journal of Veterinary & Animal Sciences

The objectives of this study were to determine the effects of copper sulphate administration on body weight gain and some biochemical, haematological parameters in lambs which had serum copper levels at subclinical values. This study was performed on 30 Ivesi male lambs (control group: 12, experimental group: 18), aged with 15-20 days. In the experimental group, 100 mg copper sulphate (1% solution) was administered orally four times (with periods of two weeks). Blood samples were collected on 0, 14, 28, 42, 56th days and body weight of lambs was determined. Copper administration caused significant increases in weight gain, haemoglobin and …

Vitamin A, Vitamin E And Selenium Levels In Blood And Milk Sera Of Cattle With Foot And Mouth Disease, Serdar Kizil, Ari̇f Altintaş

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to determine \beta-carotene, retinol (Vit A), selenium (Se) and \alpha-tocopherol (Vit E) levels in the sera of blood and milk from cattle with foot and mouth disease (FMD). A total of 120 blood sera and 22 milk sera were used for the analysis. \beta-carotene and Vit A, Se and Vit E levels were determined by spectrophotometer, fluorophotometer and HPLC respectively. Serum samples were obtained from 0-1 and 1-3-year-old cattle bred in public and state farms. Animals were grouped according to their sex, vaccination status and health or infection with FMD. Results of the biochemical …

The Effect Of Genotype On The Antioxidative Metabolism In Angora Goats, Ulvi̇ Reha Fi̇danci, Fügen Turgay, Seda Zengi̇n, Funda Kargin, Sefa Çeli̇k, Umut Taşdemi̇r

Turkish Journal of Veterinary & Animal Sciences

In this study, lipid peroxidation, intracellular, membrane and extracellular antioxidant activities in Turkish Angora goats (Genotype I) and American x Turkish crossbred Angora goats (Genotype II) were investigated, in order to show the effects of genotype on the antioxidative metabolism. Plasma malonlydialdehyde (MDA) levels of Angora goats in different genotype were analyzed to show the effects of free radicals. Glutathione peroxidase (GSH-Px), superoxide dismutase (SOD) and catalase (CAT) activities in hemolysates of erythrocytes were analyzed as intracellular antioxidants, \beta-carotene in serum as membrane antioxidants and ascorbic acid in plasma, albumin, total bilirubin, glucose, uric acid and ceruloplasmine in serum as …

Detection Of Antibodies Against Salmonella Enteritidis In Chicken Serum And Egg Yolk With Elisa, Gülay Altay, Hakan Yardimci

Turkish Journal of Veterinary & Animal Sciences

A total of 473 sera from hens of different age groups and different breeds and a total of 430 eggs were collected from 11 breeders and egg producers in the districts of Adapazarı, Afyon, Ankara and Konya. Lipopolysaccharide (LPS), heat-extract (HE) and flagella antigens were tested in the standardizing ELISA technique. Flagella antigen was found useful in the screening of field samples. Of the 48 (10.2%) rapid plate agglutination positive sera, 7 were negative in ELISA. One hundred and seventy-three (36.6%) of the 473 sera and 76 (17.7%) of the 430 egg yolks were found to be positive with ELISA. …

Photoinactivation Of Buffalo Liver And Kidney Tissue Arginases And Its Kinetic Properties, Necmi̇ Özdemi̇r, Mehtap Özçeli̇k

Turkish Journal of Veterinary & Animal Sciences

Exposure of buffalo liver and kidney tissue arginases to a 150 w light source from a distance of 8-10 cm, in the presence of 0.05mM methylene blue, caused significant inhibition of arginase activity. It was determined that this inhibition occurred differently in liver and kidney tissues. Arginase activity was spectrophotometrically measured using the Thiosemicarbazide diacetyl-monoxime urea (TDMU) method. Following preincubation with MnCl_2, exposure to a 150 w light source and the addition of methylene blue, the activity of arginase was found to be lower than that after preincubation with a 150 w light source and methylene blue and the addition …

Effects Of Administration Of Fertirelin Acetate And Gentamycin Sulphate After Artificial Insemination On Pregnancy Rate Of Cows, Ati̇lla Yildiz

Turkish Journal of Veterinary & Animal Sciences

Two studies were conducted to evaluate if Fertirelin acetate (an analogue of GnRH) and Gentamycin sulphate would improve pregnancy rate in healthy cows or in nonpregnant cows although they were inseminated three times or more. The first investigation was carried out in sixty-eight healthy cows. Cows were alloted randomly to one of two treatment groups or one of two control groups when found to be in oestrus. The first treatment group was treated with 100 micrograms of Fertirelin acetate, while the first control grup was given saline (2 ml) intramuscularly immediately after artificial insemination. The second treatment group was treated …

A Study On Some Production Traits And Egg Quality Characteristics Of Japanese Quail, Ahmet Nazligül, Kenan Türkyilmaz, Hüsnü Erbay Bardakçioğlu

Turkish Journal of Veterinary & Animal Sciences

This investigation was carried out to demonstrate some production traits and egg quality characteristics of Japanese quails (Coturnix coturnix japonica). One hundred female quails were used in the study. The experiment was carried out for six months. Egg production, egg weight, feed consumption and some egg quality characteristics were taken into consideration. Egg quality characteristics were measured at 8-24 weeks of age. Average egg production (% HD) at 8, 12, 16, 20 and 24 weeks of age was 20.5, 33.8, 63.7, 73.8 and 72.5 respectively. Average egg weight (g) and daily feed consumption (bird/feed/day) at 8, 16 and 24 weeks …

The Possibilities Of Using Sugar Beet Pulp Silage Produced By Different Methods In Lamb And Dairy Cow Rations 1 - Obtaining High Quality Sugar Beet Pulp Silage, Suphi̇ Deni̇z, Murat Demi̇rel, Şaki̇r Doğan Tuncer, Oktay Kaplan, Taylan Aksu

Turkish Journal of Veterinary & Animal Sciences

In this study, how to obtain high quality sugar beet pulp silage was investigated. Sugar beet pulp was mixed with wheat straw or grass hay in order to increase the level of dry matter to 20, 25 and 30%, and 5% molasses and different levels of urea were added (0, 1, 1.5 and 2% for wheat straw containing groups; 0, 0.5 and 1% for grass hay containing groups). The mixed materials were stored in containers of one kg for two months. At the end of the incubation period, the containers were opened and the silages were evaluated by measuring the …

Chemical Constituents Of Desmos Dumosus, Roxb., Mazdida Sulaiman

Student Works (2000-2009)

The chemical content of the 'leaves and bark of Des mos dumosus has been carried out in this study. The leaves yielded nine isoquinoline type of alkaloids and two flavones. The isoquinoline alkaloids isolated were pronuciferine 37, stepharine 39, nomuciferine 40, (-) - 3 - hydroxynomuciferine 41, norlirioferine 42, asimilobine 43, liriodenine 44, lysicamine 45 and O - methylmoschatoline 46. The flavones were 5 - hydroxy - 6,7 - dimethoxyflavone 47 and 5 - hydroxy - 7,8 - dimethoxyflavone 48. The same flavones, 47 and 48 and the alkaloids 45 and 46 were also present in the bark of this …

An Enzymatic Method For The Measurement Of Magnesium, Joseph Murphy

Theses

Magnesium is quantitatively the most important intracellular divalent cation (4), and has been found to serve in a wide range of enzymatic reactions (2). Studies have shown that up to ten percent of hospital patients may have hypomagnesaemia (6).

Atomic absorption spectrophotometry (AAS) under highly specified conditions is the de facto reference method for magnesium measurement but only about ten per cent of laboratories routinely use this method (4). This is because AAS is time-consuming, requires specialised equipment and a certain level of expertise, and may be hazardous due to the use of inflammable gases. Colourimetric methods e.g. calmagite are …

Hard Cases For Autonomy, Respect, And Professionalism In Medical Genetics, Roger B. Dworkin

Articles by Maurer Faculty

No abstract provided.

Impaired Fast-Spiking, Suppressed Cortical Inhibition, And Increased Susceptibility To Seizures In Mice Lacking Kv3.2 K+ Channel Proteins, David Lau, Eleazar Vega-Saenz De Miera, Diego Contreras, Alan Chow, Richard Paylor, Christopher S. Leonard, Bernardo Rudy

NYMC Faculty Publications

Voltage-gated K(+) channels of the Kv3 subfamily have unusual electrophysiological properties, including activation at very depolarized voltages (positive to -10 mV) and very fast deactivation rates, suggesting special roles in neuronal excitability. In the brain, Kv3 channels are prominently expressed in select neuronal populations, which include fast-spiking (FS) GABAergic interneurons of the neocortex, hippocampus, and caudate, as well as other high-frequency firing neurons. Although evidence points to a key role in high-frequency firing, a definitive understanding of the function of these channels has been hampered by a lack of selective pharmacological tools. We therefore generated mouse lines in which one …

Subcutaneous Adipose Tissue Thickness Changes Cooling Time During Cryotherapy, Jeffrey W. Otte

All-Inclusive List of Electronic Theses and Dissertations

Context: Cryotherapy is often applied using a standard treatment time. Use of a standard treatment time fails to consider the insulating effect subculaneous adipose tissue has during cooling of underlying muscle. If differences in adipose lissuc thickness alter I he effects of cryotherapy, then a standard application time may not produce a slamlard clTcct for all patients. Objective: The purpose of this study was to examine the effects of subcutaneous adipose tissue on cooling time during cryothcrapy. Design: A 1 x 4 factorial design was used in this study. The independent variable was skin-fold thickness, 0-10mm, 1 l-20mm, 2 l-30mm, …

Microbial Biofilms: From Ecology To Molecular Genetics, Mary Ellen Davey, George A. O'Toole

Dartmouth Scholarship

Biofilms are complex communities of microorganisms attached to surfaces or associated with interfaces. Despite the focus of modern microbiology research on pure culture, planktonic (free-swimming) bacteria, it is now widely recognized that most bacteria found in natural, clinical, and industrial settings persist in association with surfaces. Furthermore, these microbial communities are often composed of multiple species that interact with each other and their environment. The determination of biofilm architecture, particularly the spatial arrangement of microcolonies (clusters of cells) relative to one another, has profound implications for the function of these complex communities. Numerous new experimental approaches and methodologies have been …

Glucocorticoid Regulation Of Insulin Like Growth Factor Binding Protein-5 Gene Transcription In Human Osteoblasts, Xiaoying Wang

Loma Linda University Electronic Theses, Dissertations & Projects

Glucocorticoids (GCs) inhibit bone formation in vivo and inhibit osteoblast proliferation and collagen synthesis in vitro. These effects may be mediated by alterations in the insulin-like growth factor (IGF) system. In the present study of normal human osteoblast-like (HOB) cells, we tested the hypothesis that dexamethasone (Dex) inhibits the expression of IGF binding protein-5 (IGFBP-5). Dex decreased IGFBP-5 mRNA levels to 54% of control after 4 hr. Dex did not modify the decay of IGFBP-5 mRNA in transcriptionally arrested osteoblast cells. Dex decreased IGFBP-5 hnRNA levels to 67% of control after 2 hr, and the activity of the human IGFBP- …

Ryanodine Receptor Adaptation, Michael Fill, A. Zahradníková, Carlos A. Villalba-Galea, I. Zahradník, A. L. Escobar, S. Györke

School of Pharmacy Faculty Articles

In the heart, depolarization during the action potential activates voltage-dependent Ca²⁺ channels that mediate a small, localized Ca²⁺ influx (I_Ca). This small Ca²⁺ signal activates specialized Ca²⁺ release channels, the ryanodine receptors (RyRs), in the sarcoplasmic reticulum (SR). This process is called Ca²⁺-induced Ca²⁺ release (CICR). Intuitively, the CICR process should be self-regenerating because the Ca²⁺ released from the SR should feedback and activate further SR Ca²⁺ release. However, the CICR process is precisely controlled in the heart and, consequently, some sort of negative control mechanism(s) must exist to …

Arkansas Animal Science Department Report 2000, Zelpha B. Johnson, D. Wayne Kellogg

Arkansas Agricultural Experiment Station Research Series

Animal Science is very much devoted to youth education and development. During the past year, over 20,000 youth were involved in 4-H livestock projects. Two very successful activities that took place last year were the Mid-American Grassland Evaluation Contest and Livestock Judging Camps. The Grassland Contest is designed to teach students about grassland resource management for livestock and wildlife uses. The contest was held in Cape Girardeau, Missouri. Firstplace honors in the 4-H division went to White County, and second place honors went to Van Buren County. Two Livestock Judging Camps (Fayetteville and Hope) were conducted this past year. A …

Histological Pattern Of Ovarian Neoplasma., Zubair Ahmad, Naila Kayani, Sheema H. Hasan, Suhail Muzaffar, Muhammad Shafiq Gill

Department of Pathology and Laboratory Medicine

Abstract

Objective: To see the morphological pattern of benign and malignant ovarian neoplasms. Method: Retrospective study of all consecutive cases of ovarian neoplasms diagnosed at Aga Khan University Hospital between 1st January 1993 and 30th September 1998. Setting: The Section of Histopathology, AKUH, Karachi. Observation: Of 855 ovarian tumours 506 (59.18%) were benign and 349(40.81%) malignant. Surface epithelial - stromal tumours comprised 63.50% of all tumours. Benign cystic teratoma was the commonest benign tumour (35.17% of all benign tumours) and serous cystadenocarcinoma was the commonest malignant tumour (33.33% of all malignant tumours). Mucinous cystadenocarcinomas are more common in our population …

Granular Cell Tumor Of The Breast: An Uncommon Lesion That Mimics Carcinoma, S Muzafar, M S. Siddiqui, Naila Kayani, I K. Lodhi, Sheema H. Hasan

Department of Pathology and Laboratory Medicine

No abstract provided.

Vaccinia Virus-Mediated Glioma Gene Therapy Using P53, Il-2 And Il-12, Bing Chen

Loma Linda University Electronic Theses, Dissertations & Projects

Malignant brain tumors are generally lethal soon after their diagnosis, and the five-year survival rate for the patient is low in spite of neurosurgery, radiation therapy, and/or chemotherapy. Gene therapy has the potential to improve patient survival and quality of life by delivering specific combinations of therapeutic genes directly to the tumor for localized treatment.

We have based our cancer gene therapy for glioma on a highly attenuated variant of the Lister strain of vaccinia virus (VV) which has been proven to be a safe and effective vaccine vector. Other advantages include a wide host range, a strong promoter system, …

Chronic Hypoxia, Exercise Training, And Skeletal Muscle Capillarity: Angiogenic Regulation And Morphological Consequences, Ivan Mark Olfert

Loma Linda University Electronic Theses, Dissertations & Projects

Angiogenesis is important in health and disease. In particular, exercise training is known to increase skeletal muscle capillarity, providing there is sufficient training intensity. The stimulus for this may be intracellular hypoxia activating angiogenic growth factor gene expression. Acute hypoxia alone has been shown to increase the gene expression of several key angiogenic regulators, e.g. vascular endothelial growth factor (VEGF) and to a lesser degree transforming growth factor-β1 (TGF-β1), basic fibroblast growth factor (bFGF), and thrombospondin-1 (TSP-1), an endogenous negative angiogenic regulator. Paradoxically, however, chronic hypoxia is generally not found to increase mammalian skeletal muscle capillarity. Accordingly, we hypothesized that …