Medicine and Health Sciences Commons^™

Effect Of Forskolin On Actin Polymerization In Cultured Vascular Smooth Muscle, Mary Janay Bailey

Theses, Dissertations and Capstones

Increases in intracellular cyclic AMP levels have been shown to affect vascular smooth muscle morphology. In the present study, effects of elevated cyclic AMP levels on actin polymerization were assessed to determine whether cyclic AMP induces specific changes in cytoskeletal proteins of vascular smooth muscle cells. Forskolin was used to increase intracellular cyclic AMP levels through direct activation of adenylate cyclase. Light and Electron microscopy was employed to appraise changes in the shape of vascular smooth muscle cells. Confocal microscopy was used to reveal cytoskeletal changes after exposure to M forskolin for increasing intervals of time. Differences in the expression …

Santa Clara Magazine, Volume 43 Number 3, Winter 2001, Santa Clara University

Santa Clara Magazine

4 - UNIVERSITY RESPONDS TO TRAGEDY By SCM Staff. The September terrorist attacks had many repercussions at SCU, including the loss of at least two members of the University family.

12 - BEERS AND DOGS By Adam Breen. English Professor Terry Beers and his wife, Melissa '88, MBA '95, spend many early mornings sledding with their eight huskies.

14 - CALIFORNIA DREAMERS By Susan Vogel. Three current SCU students were dreaming of college long before many people do, thanks to dedicated mentors from the I Have A Dream Foundation .

Bulletin 4474 - Exotic Diseases - Horses, Agriculture Western Australia

Bulletins 4000 -

An exotic disease is a disease that is not present in Australia.

If an exotic disease did occur in Australia it would cause production losses, stock deaths and stop exports of primary production. When export markets are lost they can only be re-established once it is proven the disease is eradicated.

Cationic Surfactant Mediated Hybridization And Hydrophobization Of Dna Molecules At The Liquid/Liquid Interface And Their Phase Transfer, Murali Sastry, Ashavani Kumar, Mrunalini Pattarkine, Vidya Ramakrishnan, Krishna N. Ganesh

Faculty Works

Hybridization of complementary oligonucleotides mediated by a cationic surfactant at the water/hexane interface leads to hydrophobic, double-helical DNA which may be readily phase transferred to the organic phase and cast into thin films on solid substrates.

Vertical Transmission Of Kaposi's Sarcoma-Associated Herpesvirus, Hamakwa Mantina, Chipepo Kankasa, Winslow Klaskala, Brad Brayfield, James Campbell, Quijiang Du, Ganapati Bhat, Francis Kasolo, Charles Mitchell, Charles Wood

Nebraska Center for Virology: Faculty Publications

Little is presently known about the specific routes of transmission of Kaposi’s sarcoma-associated herpesvirus (KSHV) or human herpesvirus-8 (HHV-8). To investigate whether this agent might be transmitted vertically from mother to infant, we conducted a study on 89 KSHV seropositive mothers and their newborn infants. Thirteen mothers (14.6%) had KSHV DNA detected in their peripheral blood mononuclear cells (PBMC). Two of 89 samples drawn at birth from infants born to KSHV seropositive mothers had KSHV DNA detectable within their PBMC. These findings suggest that KSHV can be transmitted perinatally, but infrequently. Other routes of transmission such as horizontal transmission remain …

Submerged And Solid-State Phytase Fermentation By Aspergillus Niger: Effects Of Agitation And Medium Viscosity On Phytase Production, Fungal Morphology And Inoculum Performance, Maria Papagianni, Sue E. Nokes, Keith Filer

Biosystems and Agricultural Engineering Faculty Publications

Qualitative relationships between agitation levels and medium viscosity, Aspergillus niger morphology and phytase production were investigated in submerged and solid-state fermentations. Overall phytase production increased with increasing shaker speeds from 150 to 300 rpm, although specific growth rates and phytase production rates were higher at 150 rpm for 72 h from inoculation. Fungal morphology was greatly influenced by agitation with the morphological forms of small pellets and entangled mycelia predominating at 150 rpm, while the free filamentous form was obtained at 300 rpm. Upon inoculation of SSF, increased productivities were obtained from inocula grown at 150 rpm. A shift towards …

Re-Thinking The Summer Drenching Program, Brown Besier

Journal of the Department of Agriculture, Western Australia, Series 4

The summer drenching program has provided highly effective sheep worm control in Western Australia for many years - but recent research challenges its long term sustainability.

Trial results suggest that in large parts of the State, summer drenching is the main factor leading to the development of drench resistant worms. Alternative programs less likely to lead to drench resistance will require greater monitoring of worm burdens and panning pasture moves.

Antioxidation Activity Of Dhea And Its Mechanisms, Sun Yang, Han Rui

Pharmacy Faculty Articles and Research

Objective: The aim of this study was to determine the anti-oxidative activity of a new chemopreventive agent--dehydroepiandrosterone (DHEA), and the mechanisms of action by which DHEA protect the thymocytes and DNA from oxidative damage. Methods: Agarose gel electrophoresis, flow cytometry, single cel! gel electrophoresis, chemiluminescence assay, triazolyl blue tetrazolumbromide (MTT) colorimetry, and three dimensional collagen gel assay were used. Results: In agarose gel electrophoresis, 10 nmol/L DHEA blocked the typical DNA degradation (DNA Ladder) induced by H₂O₂. DHEA 2. 5 nmol/L and 10 nmol/L both significantly decreased the percentage of characteristic apoptotic DNA …

Molecular Cloning Of An \Alpha-Amylase Gene From Bacillus Subtilis Rskk246 And Its Expression In Escherichia Coli And In Bacillus Subtilis, Numan Özcan, Adem Altinalan, Mehmet Sai̇t Eki̇nci̇

Turkish Journal of Veterinary & Animal Sciences

Bacillus subtilis RSKK246 was found to produce approximately a 65-kDa \alpha-amylase enzyme. A gene was isolated encoding \alpha-amylase activity that corresponded to this size and was inserted into pUC18 plasmid which was transferred to Escherichia coli. An approximately 1.7kbp fragment, which contains a whole \alpha-amylase gene, was excised and inserted into pUB110 and then transferred into the different B. subtilis strains including RSKK246, RSKK243, RSKK244, YB886 and ORBAM. The a-amylase gene was cloned into the plasmids and expressed with its own promoter, and this promoter sequence seemed to function in the E. coli and in all B. subtilis strains. Specific …

An Assessment Of Prothrombotic Tendency In Humans Using Functional And Genomic Determinants In The South Eastern Irish Population, Carmel Cullen

Theses

Venous thrombosis occurs in about 1 per 1,000 individuals per year. It is a serious disorder and accounts for a significant number of hospital admissions and deaths annually. Thrombophilia describes the familial or acquired disorders of the haemostatic mechanism that predispose to thrombosis. Inherited thrombophilia can be defined as a genetically determined tendency to venous thromboembolism Mutations in genes that code for proteins involved directly (or indirectly) In blood coagulation have been associated with prothrombotic status. Laboratory investigations for thrombophilia include screening for deficiencies of antithrombin, protein C or protein S and testing for activated protein C resistance. Together these …

Seroprevalance Of Babesia Ovis In Sheep In Malatya, Müni̇r Aktaş, Ali̇ Düzgün, Cahi̇t Babür

Turkish Journal of Veterinary & Animal Sciences

This study was carried out in Malatya and vicinity to determine the seroprevalance of Babesia ovis in sheep. A total of 220 sheep sera were tested against B. ovis using the Enzyme-Linked Immunosorbent Assay (ELISA). In addition, peripheral blood smears from these sheep were prepared and examined for B. ovis. The ELISA test showed that 55.9% of sheep were seropositive for B. ovis infection. The infection rate was 49.2% in sheep which were 1-12 months old and 59% in sheep which were older than one year. In the microscopic examination of blood smears, four out of 220 sheep were found …

A Study On The Fattening Performance Of Tushin Lambs Subjected To Intensive Feeding, Mevlüt Karaoğlu, Muhli̇s Maci̇t, Ayhan Aksoy

Turkish Journal of Veterinary & Animal Sciences

Some characteristics related with the fattening performance of Tushin lambs reared in the Research and Application Farm of Ataturk University and subjected to intensive fattening were determined. The inital fattening weight, final fattening weight, and total and daily weight gain during the fattening period were 19.71 kg, 38.07 kg, 18.35 kg and 203.48 g respectively. The feed conversion ratio (kg feed consumed/kg live weight gain) was 5.01. The effect of year on final fattening weight, total weight gain and average daily weight gain was found to be significant (p

Some Microbiological, Chemical And Physical Characteristics Of Pastırma Marketed In Erzurum, Muhammet İrfan Aksu, Mükerrem Kaya

Turkish Journal of Veterinary & Animal Sciences

In this study, 48 pastırma samples collected from 6 different retailers were examined. It is concluded that lactic acid bacteria, Micrococcus and Staphylococcus were found to be dominant in the flora. From the microbiologic analyses, Micrococcus/Staphylococcus, lactic acid bacteria, yeast and mould counts were determined as 4.00-7.45 log CFU/g, 3.75-7.89 logCFU/g,

Diagnosis And Treatment Of Intussusception In A Cow, Murat Dabak, Emi̇ne Ünsaldi, Yusuf Gül

Turkish Journal of Veterinary & Animal Sciences

An 8-year-old cow admitted to the Veterinary Faculty, Internal Medicine Clinic (University of Fırat, Elazığ/TURKEY), was diagnosed with intussusception, and the diagnosis was confirmed by experimental laparotomy. Intussusception was corrected by enterectomy and intestinal anastomosis. Defecation on the day following the operation indicated normal intestinal flow. The animal was kept under observation for ten days after the operation, and had a full recovery.

The Determination Of Ensiled Qualities Of Corn, Alfalfa And Sugar Beet Pulp And The Effects On Food Intake And Nutrient Digestibility When Fed To Lambs In Different Forms, Pinar Tatli, İ. Hali̇l Çerçi̇, Fuat Gürdoğan

Turkish Journal of Veterinary & Animal Sciences

This study was conducted in two stages to determine the ensiled qualities of corn, alfalfa and sugar beet pulp, and the effects on food intake and nutrient digestibility of corn and alfalfa silages used alone, together and with sugar beet pulp silage in lambs. In the first stage, corn, alfalfa and sugar beet pulp were ensiled and silos were opened after 45 days. Silage samples were taken for determination of silage quality and the samples were analysed. Dry matter (DM) levels of corn, alfalfa and sugar beet pulp silages were found to be 28.56 and 31.44, 16.74%, respectively. The pH, …

Seroprevalence Of Toxoplasmosis Gondii In Sheep In Yozgat, Turkey, Cahi̇t Babür, Berri̇n Esen, Gülay Vural

Turkish Journal of Veterinary & Animal Sciences

The sera collected from 152 sheep older than one year old sheep in various parts of Yozgat Province were tested for Toxoplasma gondii antibodies using the Sabin- Feldman dye test. Out of the 152 sera examined, 69 (45.4%) were seropositive at different dilutions (34 samples at 1/16, 21 at 1/64, 11 at 1/256 and 3 at 1/1024).

A Study On Direct And Indirect Effects Of Some Factors On Weaning Weight Of A Awassi Lambs, Mehmet Topal, Nuri̇ni̇sa Esenbuğa

Turkish Journal of Veterinary & Animal Sciences

The correlation coefficients divided into direct and indirect effects among ewe weight in the mating period and lambing, birth weight and weaning age of lamb, and weaning weight of Awassi lambs raised in Erzurum conditions were determined in this study. In path analysis, the direct and indirect impact of birth weight and weaning age of lamb on weaning weight were found to be highly significant. Partial correlation and standardized partial regression coefficients among weaning weight, birth weight and weaning age of lamb were also observed to be highly significant.

The Diagnosis Of Antibodies Against Newcastle Disease Virus By Hi And Elisa Tests In Vaccinated Chickens, Nejat Aydin, Hakan Yardimci, K. Semi̇h Gümüşsoy, Belgi̇n Erdem

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to determine serum antibody levels against the Newcastle disease virus in vaccinated chicken flocks in addition to experimental animals by hemagglutination inhibition (HI)test and enzyme linked immunosorbent assay (ELISA) and compare the results. Methanol-precipitated antigen prepared from the LaSota strain of Newcastle disease virus was used with peroxidase-labelled rabbit anti-chicken IgG as a conjugate and ortho-phenylenediamine as a substrate in ELISA. The negative threshold was found to be an optical density of 0.19 (OD)at a 490-nm wavelength.In addition to the 50 experimental animals, a total of 504 chicken sera (broiler,layer and breeder flocks)collected from …

The Presence And Prevalence Of Listeria Species In Milk And Herby Cheese In And Around Van, Emrullah Sağun, Yakup Can Sancak, Özgür İşleyi̇ci̇, Kami̇l Eki̇ci̇

Turkish Journal of Veterinary & Animal Sciences

In this study 250 raw milk and 254 herby cheese samples collected from Van city center and neighboring villages were investigated in terms of Listeria species. For Listeria isolation,the method recommended by the FDA was used.Of the raw milk samples,6 (2.40 %)were found to be positive with regard to Listeria; 3 (1.20 %)had L.monocytogenes, 1 (0.40 %)L.innocua and 1 (0.40 %)L.welshimeri. Of the herby cheese samples,13 (5.11 %)were found to be positive with regard to Listeria;10 (3.93 %)had L.monocytogenes, 1 (0.39 %)L.ivanovii,1 (0.39 %)L.innocua and 1 (0.39 %)L.welshimeri. For the serotype determination of 13 isolates defined as L.monocytogenes Difco Bacto …

Comparison Of Elisa With Other Serological Tests In The Diagnosis Of Ovine Brucellosis, Hasan Öngör, Adi̇le Muz, Burhan Çeti̇nkaya

Turkish Journal of Veterinary & Animal Sciences

In this study,antibodies against Brucella melitensis in aborted sheep were investigated with enzyme-linked immunosorbent assay (ELISA), standard tube agglutination test (SAT), rose bengal plate test (RBPT), complement fixation test (CFT), 2-mercaptoethanol microagglutination test (2-ME-MAT)and dithiothreitol microagglutination test (DTT-MAT). The sensitivity and specificity of the tests were calculated by using CFT results as the gold standard. A Kappa value was used to measure the agreements between ELISA and the other tests. Sonicated antigen prepared from Brucella melitensis 16M strain,the peroxidase labelled anti-sheep IgG conjugate and ortho-phenylenediamine substrate were used in ELISA. A total of 500 blood samples collected from 36 sheep …

Pathomorphologic Investigations On The Genital System Of Ewes Ii.Uterus, Cervix And Vagina, Erkan Karadaş, Necati̇ Ti̇murkan

Turkish Journal of Veterinary & Animal Sciences

This study was carried out to determine the pathomorphologic changes and the incidence of these changes in the uterus, cervix, vagina and related ligaments of ewes slaughtered at Elazığ abattoirs. For this purpose,the genital organs of 6100 ewes from different breeds and ages were examined.There were pathomorphologic changes in 274 cases (4.49%)in the uterus,70 cases (1.14%)in the cervix,and 61 cases (1.00%)in the vagina.Inflammatory changes were primarily lesions (2.68%)observed in the uterus.These changes were catarrhal endometritis (0.52%), purulent metritis (0.32%),necrotic endometritis (0.11%), chronic nonpurulent endometritis (0.95%),granulomatous endometritis (0.13%),pyometra (0.11 %)and parametritis (0.52%). In addition,endometrial hyperplasia (0.36%), hydrometra (0.34%), uterus unicornis (0.16%), …

The Incidence Of Coxiella Burnetii Infection In Ewes In Elazığ And Neighboring Provinces, Hakan Kalender

Turkish Journal of Veterinary & Animal Sciences

This study was carried out to determine the incidence of C.burnetii infection using the Indirect Fluorescent Antibody (IFA)Test in ewes in Elazığ and neighboring provinces.Seropositivity was detected in 38.59 %(71/184)of aborted ewes and in 11.01 %(25/227)of unaborted ewes.The seropositivity rates were 20 %in Elazığ,20% in Malatya, 27.95 %in Bingöl and 27.27% in Muş. Antibody titers ranged from 1/80 to 1/1280. Q fever should be considered an important cause of abortions in ewes in Elazığ and neighboring provinces.

Studies On The Effect Of Bovine Somatotropin Hormone (Rbst)On Milk Yield, Milk Composition And Health Of Dairy Cows, İsmai̇l Abaş, Haydar Özpinar

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to investigate the influence of growth hormone (rbST)on milk yield, milk composition, animal health and sera concentration of glucose, GOT, GPT, insulin, free fat acids and blood urea of Holstein dairy cows. In this study,20 primiparous Holstein dairy cows were treated with rbST or placebo (control)in weeks 10-18 and 20-28 of lactation. In the first treatment period, the ten cows of the treatment Group A were administrated 500 mg methionyl bovine growth hormone, and the other ten cows (Group B)the same quantity of placebo.The treatment with growth hormone or placebo was switched over in …

The Effects Of Amitraz On The Motility Of Guinea Pig Small Intestine, Vedat Sağmanligi̇l, İlksi̇n Pi̇şki̇n, Meltem Şi̇reli̇, Ayhan Fi̇lazi̇

Turkish Journal of Veterinary & Animal Sciences

In this study, 14 adult albino guinea pigs, weighing 300-350 g, were divided into two equal groups. The small intestinal segments (distal ileum)taken from the animals were kept in vitro in the isolated organ bath, and the isotonic contractions of the tissues produced by amitraz and acetylcholine were recorded on a polygraph. For the first group, tissues belonging to the seven animals, the cumulative concentration-response curves of amitraz were obtained and compared by applications at concentrations from 10^{-8} M to 10^{-3} M in three different conditions alone,in the presence of verapamil (3x10^{-5} M)and in Ca^{++} free solution. For the second …

Transferrin Polymorphism In Hair Goat Breeds Raised In The Bursa Region, Cengi̇z Elmaci, Mehmet Koyuncu, Erdoğan Tuncel

Turkish Journal of Veterinary & Animal Sciences

In this study transferrin polymorphism was investigated in hair goat breeds raised in the Bursa region by using 148 blood samples of hair goat. The separation of transferrin types was carried out using horizontal starch-gel electrophoresis. The frequencies of transferrin alleles were calculated to be 0.54 ±0.029 and 0.46 ±0.029 for Tf A and Tf B , respectively. Differences between the expected and observed number of transferrin genotypes were insignificant.

The Relationships Between Transferrin (Tf)Types And Some Wool Characteristics In Akkaraman And Anatolian Merino Ewes, Gürsel Dellal

Turkish Journal of Veterinary & Animal Sciences

In this research,the relationships between transferrin types and some wool characteristics in Akkaraman and Anatolian Merino ewes were investigated.The separation of transferrin types was carried out with horizontal starch-gel electrophoresis in a discontinuous system.There was no statistical relationship between transferrin types and wool characteristics.

In Vitro Estimation Of The Solubility Of Dry Matter And Crude Protein Of Wet Feed And Dry, Sulhatti̇n Yaşar, John Mike Forbes

Turkish Journal of Veterinary & Animal Sciences

Two experiments were conducted to determine the in vitro solubility of dry matter and crude protein of wet feed and dry feed of a commercial broiler diet. In the first experiment, to two g of feed samples of a commercial broiler pelleted diet was added 0-dry (80 g/kg water content) and 1.5 g water per g of feed (640 g/kg water content), and they were then incubated at one of two different levels of pepsin-HCl solutions, low (48 mg pepsin in 2 ml 0.1M HCl), or high (64 mg pepsin in 3 ml 0.1M HCl) for 0, 15, 30, 60, …

Prevalence Of Cryptosporidium Spp. Oocysts In Diarrhoeic Calves In Kars Province, Turkey, M. Özkan Arslan, Yunus Gicik, H. Meti̇n Erdoğan, Bariş Sari

Turkish Journal of Veterinary & Animal Sciences

This study was carried out to determine the prevalence of Cryptosporidium spp. oocysts in diarrhoeic calves in Kars province. The study was conducted between February 1999 and June 1999, involving 140 diarrhoeic calves from 8 different localities (Kars-Central district, Karakaş, Bulanık, Karakale, Hacıhalil, Boğazköy, Susuz-Central district, and Arpaçay). These localities were visited once during the study period, and fresh faecal samples were taken from the rectums of the calves up to 3 months old. Faecal samples were centrifuged and the resulting sediments were smeared on glass slides. The slides were stained by the modified acid-fast technique and examined under light …

Seasonal Variation In Bovine Luteal Concentrations Of Ss-Carotene, Şevket Arikan, Richard G. Rodway

Turkish Journal of Veterinary & Animal Sciences

ß-Carotene is present in extremely high concentrations in bovine corpus luteum. The aim of this study was to examine the extent of the seasonal variation in concentrations of luteal ß-carotene in cows. A total of 102 corpora lutea collected throughout all 12 months of the year were analysed by high performance liquid chromatography (HPLC). The mean ß-carotene concentration of the luteal tissues was 58.7 ± 4.5 µg/g. Bovine corpora lutea collected in the warmer months (May-Oct.) contained significantly (P

The Effects Of Aging Period And Muscle Type On The Textural Quality Characteristics Of Mutton, Mete Yanar, Hasan Yeti̇m

Turkish Journal of Veterinary & Animal Sciences

In this research, the effects of aging on some of the textural quality characteristics of Longissimus dorsi (LD) and Semimembranosus (SM) muscles from mutton carcasses were evaluated. The results showed that the aging process significantly (P