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Vitrification Of In Vitro-Produced Bovine Embryos Matured Inmodified Tcm-199 Medium, Asi̇ye İzem Sandal, Özen Banu Özdaş
Vitrification Of In Vitro-Produced Bovine Embryos Matured Inmodified Tcm-199 Medium, Asi̇ye İzem Sandal, Özen Banu Özdaş
Turkish Journal of Veterinary & Animal Sciences
In vitro-produced bovine embryos matured in modified Tissue Culture Medium 199 (TCM-199) were vitrified at the 7th and 8th days of culture, and development at 48 h after warming was recorded. Ovaries were obtained from a local abattoir, and the oocytes were recovered by slicing method and divided into two main groups. The first group included cysteamine in the TCM-199 (Group A) and the second did not (Group B). They were incubated for 24 h at 38.8 °C in an atmosphere of 5% CO2 in humidified air. Matured oocytes were fertilized with 1-2 × 106/mL of frozen-thawed bull semen. At …
The Effect Of Macromolecule And Growth Factor Combinations On In Vitrodevelopment Of Bovine Embryos, Alper Koçyi̇ği̇t, Mesut Çevi̇k, Uğur Şen, Mehmet Kuran
The Effect Of Macromolecule And Growth Factor Combinations On In Vitrodevelopment Of Bovine Embryos, Alper Koçyi̇ği̇t, Mesut Çevi̇k, Uğur Şen, Mehmet Kuran
Turkish Journal of Veterinary & Animal Sciences
This study was conducted to determine the effects of different macromolecule sources added to synthetic oviduct fluid (SOF) culture medium supplemented with growth factors on the development of bovine embryos and blastocyst morphology. Zygotes were distributed into 5 treatment groups. Cleavage, morula, and blastocyst rates were evaluated under a stereomicroscope. Trophectoderm (TE) and inner cell mass (ICM) cells were determined by differential staining method. It was found that bovine serum albumin (BSA), either alone or in combination with growth factors, as compared to the control or polyvinyl-alcohol (PVA) resulted in higher embryo yield and faster development during early bovine embryo …