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Full-Text Articles in Medicine and Health Sciences
Seroprevalence Of Bovine Viral Diarrhoea Virus, Bovine Herpesvirus Type 1 And Bovineherpesvirus Type 4 Infections In Cattle In Ağrı Province, Eastern Anatolia Region, Turkey, Mustafa Si̇nan Aktaş, Hasan Çeli̇k
Seroprevalence Of Bovine Viral Diarrhoea Virus, Bovine Herpesvirus Type 1 And Bovineherpesvirus Type 4 Infections In Cattle In Ağrı Province, Eastern Anatolia Region, Turkey, Mustafa Si̇nan Aktaş, Hasan Çeli̇k
Turkish Journal of Veterinary & Animal Sciences
This research was carried out to determine the seroprevalence of Bovine Viral Diarrhoea Virus (BVDV), Bovine Herpes Virus 1 (BoHV-1), and Bovine Herpes Virus 4 (BoHV-4) in cattle (n = 188) in Ağrı province of Eastern Anatolia Region, Turkey. The presence of BVDV, BoHV-1, and BoHV-4 antibodies in sera samples were determined by an ELISA test kit. It was determined that the highest seropositivity for BoHV-1 in Tutak disrict (9.9%), in ≤ 3 years age groups (3.39%), and in Simmental cattle (14.28%); for BoHV-4 in Doğubeyazıt disrict (4.16%), %), in ≤ 3 years age groups (1.70%), and in Simmental cross …
Oocyte Recovery And In Vitro Embryo Production In Cows Treated With A Single Dose Offollicle-Stimulating Hormone, Thiago Marquez Fernandes, Paulo Roberto Adona, Samuel Guemra, Tiago Henrique Camara De Bem, Moyses Dos Santos Miranda
Oocyte Recovery And In Vitro Embryo Production In Cows Treated With A Single Dose Offollicle-Stimulating Hormone, Thiago Marquez Fernandes, Paulo Roberto Adona, Samuel Guemra, Tiago Henrique Camara De Bem, Moyses Dos Santos Miranda
Turkish Journal of Veterinary & Animal Sciences
Cows were treated with follicle-stimulating hormone (FSH) in a single dose and then subjected to ovum pick-up (OPU) for evaluation of oocyte recovery and in vitro fertilization (IVF). Cows underwent OPU followed by application of FSH (200 mg) on day 2 and new OPU on day 3. This methodology was repeated 3 times sequentially. In FSH-free the cows (control), OPU was performed at intervals of one week. The number of oocytes recovered was higher in the control cows (22.2 ± 1.5) compared to the treated cows (16.9 ± 1.4; P < 0.05). There was no difference between the control (13.2 ± 0.6) and the treated (11.2 ± 1.1) cows in the number of viable oocytes (P > 0.05). There was no difference between the control cows (47.1 …
Comparative Efficacy Of Antioxidant Retinol, Melatonin, And Zinc During In Vitro Maturation Of Bovine Oocytes Under Induced Heat Stress, Jafrin Ara Ahmed, Devojyoti Dutta, Nawab Nashiruddullah
Comparative Efficacy Of Antioxidant Retinol, Melatonin, And Zinc During In Vitro Maturation Of Bovine Oocytes Under Induced Heat Stress, Jafrin Ara Ahmed, Devojyoti Dutta, Nawab Nashiruddullah
Turkish Journal of Veterinary & Animal Sciences
Both A and B grade cumulus-oocyte complexes (COCs) aspirated from cattle ovaries at slaughter were matured in vitro under normal (38.5 °C) and elevated temperatures (41 °C). Maturation competence based on cumulus expansion, COC diameter, and nuclear maturation were compared with and without antioxidant supplementation incorporating 7.5 µM retinol, 1 nM melatonin, and 1.5 µg/mL zinc chloride in an oocyte maturation medium. Heat stress significantly reduced cumulus expansion by approximately 20%, while only retinol could bring a significant (P ≤ 0.05) increase. Heat stress also decreased expansion of A (approximately 50%) and B (approximately 40%) grade COC diameter. All antioxidants …
Vitrification Of In Vitro-Produced Bovine Embryos Matured Inmodified Tcm-199 Medium, Asi̇ye İzem Sandal, Özen Banu Özdaş
Vitrification Of In Vitro-Produced Bovine Embryos Matured Inmodified Tcm-199 Medium, Asi̇ye İzem Sandal, Özen Banu Özdaş
Turkish Journal of Veterinary & Animal Sciences
In vitro-produced bovine embryos matured in modified Tissue Culture Medium 199 (TCM-199) were vitrified at the 7th and 8th days of culture, and development at 48 h after warming was recorded. Ovaries were obtained from a local abattoir, and the oocytes were recovered by slicing method and divided into two main groups. The first group included cysteamine in the TCM-199 (Group A) and the second did not (Group B). They were incubated for 24 h at 38.8 °C in an atmosphere of 5% CO2 in humidified air. Matured oocytes were fertilized with 1-2 × 106/mL of frozen-thawed bull semen. At …
The Effect Of Macromolecule And Growth Factor Combinations On In Vitrodevelopment Of Bovine Embryos, Alper Koçyi̇ği̇t, Mesut Çevi̇k, Uğur Şen, Mehmet Kuran
The Effect Of Macromolecule And Growth Factor Combinations On In Vitrodevelopment Of Bovine Embryos, Alper Koçyi̇ği̇t, Mesut Çevi̇k, Uğur Şen, Mehmet Kuran
Turkish Journal of Veterinary & Animal Sciences
This study was conducted to determine the effects of different macromolecule sources added to synthetic oviduct fluid (SOF) culture medium supplemented with growth factors on the development of bovine embryos and blastocyst morphology. Zygotes were distributed into 5 treatment groups. Cleavage, morula, and blastocyst rates were evaluated under a stereomicroscope. Trophectoderm (TE) and inner cell mass (ICM) cells were determined by differential staining method. It was found that bovine serum albumin (BSA), either alone or in combination with growth factors, as compared to the control or polyvinyl-alcohol (PVA) resulted in higher embryo yield and faster development during early bovine embryo …
Ultrasonographic Evaluation Of Massive Abdominal Wall Swellings In Cattle And Buffaloes, Ashwani Kumar, Jitender Mohindroo, Vandana Sangwan, Shashi Kant Mahajan, Kiranjeet Singh, Arun Anand, Narinder Singh Saini
Ultrasonographic Evaluation Of Massive Abdominal Wall Swellings In Cattle And Buffaloes, Ashwani Kumar, Jitender Mohindroo, Vandana Sangwan, Shashi Kant Mahajan, Kiranjeet Singh, Arun Anand, Narinder Singh Saini
Turkish Journal of Veterinary & Animal Sciences
Eight cows and 7 buffaloes with massive abdominal wall swellings were examined. The aim of the ultrasonographic study was to learn the contents of the abdominal wall swellings. They were divided into 4 groups: Group I (prepubic tendon rupture or hernia), Group II (fibrino-cystic swelling), Group III (abscess), and Group IV (inflammatory swelling). Palpation of the swelling in semidorsal recumbency, needle aspiration, or surgery was used to confirm the diagnosis. In animals of Groups I, II, and III, in the ultrasound, the muscles appeared to separate from the skin at the margin of swelling, thus creating a gap. In this …
Study Of Insulin-Like Growth Factor 1 Effects On Bovine Type A Spermatogonia Proliferation And Viability, Babak Qasemi Panahi, Parviz Tajik, Mansoureh Movahedin, Gholamali Moghaddam, Mohammad Hossein Geranmayeh
Study Of Insulin-Like Growth Factor 1 Effects On Bovine Type A Spermatogonia Proliferation And Viability, Babak Qasemi Panahi, Parviz Tajik, Mansoureh Movahedin, Gholamali Moghaddam, Mohammad Hossein Geranmayeh
Turkish Journal of Veterinary & Animal Sciences
Spermatogonial stem cells (SSCs) are the best candidates for exploitation in genetic improvement of cattle herds. In fact, SSCs transmit genetic information to future generations. In vitro improvement in proliferation and frozen-thawed SSCs numbers is therefore needed. In the present study, bovine SSCs were cocultured with Sertoli cells, with or without insulin-like growth factor 1 (IGF-1). During 16 days of incubation, 100 ng/mL IGF-1 showed higher efficacy in increasing the numbers and diameters of SSCs colonies than 40 and 150 ng/mL concentrations. Furthermore, 100 ng/mL IGF-1 increased the frozen/thawed cells’ viability rate. Overall, these data demonstrate that usage of 100 …
Clinical Characteristics, Hematology, And Biochemical Analytes Of Primary Omasal Impaction In Bovines, Syed Aashiq Hussain, Sanjeev Kumar Uppal, Charanjit Randhawa, Naresh Kumar Sood, Shashi Kant Mahajan
Clinical Characteristics, Hematology, And Biochemical Analytes Of Primary Omasal Impaction In Bovines, Syed Aashiq Hussain, Sanjeev Kumar Uppal, Charanjit Randhawa, Naresh Kumar Sood, Shashi Kant Mahajan
Turkish Journal of Veterinary & Animal Sciences
This study was conducted on 11 animals (7 buffaloes and 4 cows) diagnosed with primary omasal impaction. In all the cases, laparorumenotomy was performed and omasal impaction was confirmed based on the consistency of the omasal contents along with an empty abomasum. Signalment, history, clinical signs, clinic-pathologic findings, and treatment were determined. Clinical examination was found to be helpful in subjective assessment of omasal impaction but could not aid in definitive diagnosis. Pain on palpation of the omasal area along with rectal findings could be a diagnostic feature. Neutrophilic leukocytosis was observed in a majority of animals. Total bilirubin, glucose, …
Immunodetection Of Insulin-Like Growth Factor Binding Proteins (Igfbps) In The Sera Of Different Animal Species, Romana Masnikosa, Ivona Baricevic, Dragana Lagundzin, Olgica Nedic
Immunodetection Of Insulin-Like Growth Factor Binding Proteins (Igfbps) In The Sera Of Different Animal Species, Romana Masnikosa, Ivona Baricevic, Dragana Lagundzin, Olgica Nedic
Turkish Journal of Veterinary & Animal Sciences
Knowledge regarding the insulin-like growth factor (IGF) system and the methods for the detection and quantitation of IGF binding proteins (IGFBPs) is greater for the human species than for other animal species. This is mainly because antibodies specifically raised against nonhuman IGFBPs are rarely commercially available. We aimed to investigate which of the antibodies directed against human IGFBPs could be used for the detection of IGFBPs present in other species. We used commercially available antibodies, raised against human IGFBP-1 through IGFBP-4, in order to recognize the corresponding IGFBPs in the sera of different animal species: human, bovine, equine, porcine, ovine, …
Determination Of Optimal Dose Of Egf For Bovine Oocyte Maturation And Subsequent In Vitro Fertilization And Culture In Two Media, Ayhan Baştan, Bülent Polat, Duygu Baki̇ Acar, Ömer Korkmaz, Armağan Çolak
Determination Of Optimal Dose Of Egf For Bovine Oocyte Maturation And Subsequent In Vitro Fertilization And Culture In Two Media, Ayhan Baştan, Bülent Polat, Duygu Baki̇ Acar, Ömer Korkmaz, Armağan Çolak
Turkish Journal of Veterinary & Animal Sciences
The aim of this study was to determine the optimal dose of EGF on in vitro maturation of bovine oocytes and to investigate the effect of different IVF and IVC media on development of bovine embryos matured with EGF. Oocytes collected from a slaughterhouse were randomly divided into 4 groups. Each group of oocytes was incubated with different doses of EGF (Control: 0 ng/mL, Group 1: 1 ng/mL, Group 2: 10 ng/mL, and Group 3: 100 ng/mL) for 24 h to determine the optimal dose. Matured oocytes were fertilized with frozen-thawed bull sperm by using the swim-up method. The oocytes …
Detection Of Bovine Tuberculosis By Tuberculin Test And Polymerase Chain Reaction In Van, Turkey, Hasan Solmaz, Zi̇ya İlhan, Abdulbaki̇ Aksakal, Ti̇mur Gülhan, İsmai̇l Hakki Eki̇n
Detection Of Bovine Tuberculosis By Tuberculin Test And Polymerase Chain Reaction In Van, Turkey, Hasan Solmaz, Zi̇ya İlhan, Abdulbaki̇ Aksakal, Ti̇mur Gülhan, İsmai̇l Hakki Eki̇n
Turkish Journal of Veterinary & Animal Sciences
The aim of this study was to identify the presence and prevalence of bovine tuberculosis in the eastern Anatolian border province of Van and its villages and to collect the preliminary data required for programs for the prevention and control of this zoonotic disease. The tuberculin test was performed on 210 cattle. Nasal swab and milk samples were obtained from the animals tested. Three cattle that had a positive tuberculin test also tested positive for the DNA target, a 580-bp fragment of IS6110 specific for members of the Mycobacterium tuberculosis complex. This fragment was also recovered from milk taken from …
Seroprevalence Of Brucellosis In Human, Cattle, And Buffalo Populations In Pakistan, Iftikhar Hussain, Muhammad Imran Arshad, Muhammad Shahid Mahmood, Masood Akhtar
Seroprevalence Of Brucellosis In Human, Cattle, And Buffalo Populations In Pakistan, Iftikhar Hussain, Muhammad Imran Arshad, Muhammad Shahid Mahmood, Masood Akhtar
Turkish Journal of Veterinary & Animal Sciences
A total of 1500 serum samples were collected from cattle, buffaloes, and humans. The Rose Bengal plate test (RBPT) and enzyme-linked immunosorbent assay (ELISA) detected Brucella antibodies in 56 (10.18%) and 44 (8%) of the cattle, respectively. In samples collected from buffaloes 9.38% and 6.92% were positive, whereas 14% and 11% of the samples from humans were found to be positive by RBPT and ELISA, respectively. Among village workers a higher prevalence of brucellosis was recorded in females than in males. Abattoir-associated personnel also had a higher incidence of brucellosis. Results of the present study revealed that RBPT and ELISA …
The Influence Of Epidermal Growth Factor On Maturation And Fertilisation Of Bovine Oocytes In Vitro, Bülent Polat, M. Rifat Salmanoğlu
The Influence Of Epidermal Growth Factor On Maturation And Fertilisation Of Bovine Oocytes In Vitro, Bülent Polat, M. Rifat Salmanoğlu
Turkish Journal of Veterinary & Animal Sciences
The aim of the present study was to evaluate the efficiency of different doses of epidermal growth factor (EGF) on maturation and fertilisation of bovine oocytes in vitro. A total of 1209 high quality oocytes (follicles 2-8 mm in diameter, with unexpanded cumulus cells) were obtained by aspiration from ovaries collected at a slaughterhouse. The oocytes were then assigned into 6 groups: 1, 10, 100, and 1000 ng/ml EGF groups, as well as positive (EGF replaced with hCG) and negative (TCM 199 only, no supplement) control groups. Maturation and fertilisation rates were calculated at 39 °C in an atmosphere of …
Plasmids And Protein Patterns Of Escherichia Coli Isolated From Bovine Mastitis In Konya, Turkey, Uçkun Sai̇t Uçan, Leyla Açik, Ayten Çelebi̇, Osman Ergani̇ş, Emi̇ne Arslan
Plasmids And Protein Patterns Of Escherichia Coli Isolated From Bovine Mastitis In Konya, Turkey, Uçkun Sai̇t Uçan, Leyla Açik, Ayten Çelebi̇, Osman Ergani̇ş, Emi̇ne Arslan
Turkish Journal of Veterinary & Animal Sciences
In this study, a total of 30 Escherichia coli isolates obtained from milk samples of dairy cows suffering from subclinical mastitis in Konya, Turkey were typed according to plasmids and protein patterns. Agarose gel electrophoresis and sodium doedecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) methods were used to identify plasmids and whole-cell protein profiles. Of these two methods, SDS-PAGE typing proved to be more discriminate for typing the isolates.
Meningitis Associated With Brucella Abortus In An Aborted Bovine Foetus, Cengi̇z Çeti̇n, İ. Taci̇ Cangül, Mehmet Akan, Ebru Özel, Ahmet Akkoç
Meningitis Associated With Brucella Abortus In An Aborted Bovine Foetus, Cengi̇z Çeti̇n, İ. Taci̇ Cangül, Mehmet Akan, Ebru Özel, Ahmet Akkoç
Turkish Journal of Veterinary & Animal Sciences
In this study, the brain and other organs from an aborted bovine foetus were examined histopathologically and microbiologically. In addition, a serum sample from the aborting cow was examined serologically. A granulomatous inflammation, characterised by the infiltration of lymphocytes, plasma cells with some neutrophils and macrophages was observed in the meninges of the brain, liver and kidney. Brucella abortus biovar 1 was isolated in pure culture from the brain and other organs. The serum sample was positive for brucellosis. These findings indicate that Brucella abortus is a pathogen capable of inducing meningitis in bovine foetuses.
Immunoperoxidase Studies On Leptospirosis In Bovine Abortion, Ahmet Temur, Yavuz Seli̇m Sağlam
Immunoperoxidase Studies On Leptospirosis In Bovine Abortion, Ahmet Temur, Yavuz Seli̇m Sağlam
Turkish Journal of Veterinary & Animal Sciences
This study was conducted to determine leptospirosis from aborted bovine fetuses. Tissue sections of the liver, lung, spleen and kidney were stained with avidin-biotin peroxidase complex, and antigen localization was examined. These examinations showed positive staining in eight (24.24%) out of 33 fetuses for the presence of leptospira antigens. In these eight cases, leptospira antigens were found in three lung (37.5%), two liver (25%), four kidney (50%) and four spleen (50%) samples. Microscopic studies demonstrated that leptospiral antigens were located in the interalveolar and interlobular septum of the lung; in the cytoplasm of hepatocytes and macrophages in the portal regions …
A Comparison Of The Effect Of Methyl-B-Cyclodextrin On The Osmotic Fragility Of Ovine, Bovine And Human Erythrocytes, Şevket Arikan
A Comparison Of The Effect Of Methyl-B-Cyclodextrin On The Osmotic Fragility Of Ovine, Bovine And Human Erythrocytes, Şevket Arikan
Turkish Journal of Veterinary & Animal Sciences
Cyclodextrins are used extensively in food, cosmetic, drug and chemical industries to increase aqueous solubility and stability, and reduce or eliminate the unpleasant taste and smell of many products. Experiments were conducted to examine the effect of a pre-haemolytic dose of methyl b-cyclodextrin (MBCD) on the osmotic fragility of erythrocytes collected from sheep, cattle and humans. Heparinised blood collected from these three species were analysed within 3 h of collection. Erythrocyte suspensions were mixed with MBCD to give concentrations of 2 mM, 4 mM or 6 mM cyclodextrin. The mixtures were incubated for 30 min at 37 °C and centrifuged. …
The Presence, Isolation And Characterisation Of Galectin-1 In Bovine Adrenal Gland, Kami̇l Seyrek
The Presence, Isolation And Characterisation Of Galectin-1 In Bovine Adrenal Gland, Kami̇l Seyrek
Turkish Journal of Veterinary & Animal Sciences
The mammalian adrenal gland in the developmental stage provides excellent system for studying the role(s) of lectins in the foetal period because the adrenal gland is composed of two separate endocrine organs which differ in embryologic origin, type of secretion, and function. There is no information on the presence of galectin-1 or galectin-1 reactive glycoconjugates in the development of the bovine adrenal gland. Therefore, the present study focused on this protein. For separation of galectin-1 dialysis, affinity chromatography and SDS-PAGE were used. To monitor the localisation of galectin-1 and its epitopes immunhistochemistry was used. A single 14-kD band comigrated with …
Transgenic Bovine Nuclear Transfer Embryos From Adult Somatic Cell Lines*, Sezen Arat, S. Jacek Rzucidlo, Steven L. Stice
Transgenic Bovine Nuclear Transfer Embryos From Adult Somatic Cell Lines*, Sezen Arat, S. Jacek Rzucidlo, Steven L. Stice
Turkish Journal of Veterinary & Animal Sciences
This study was performed to examine whether adult fibroblast cells can be manipulated genetically and whether clonal lines derived from those cells can support embryonic development. A primary adult cell line was established from the ear of an aged cow. Adult cells with a plasmid containing the enhancer green fluorescence protein (EGFP) gene, and non-transfected cells were used for cloning. Green fluorescence expression was observed in 35/49 (71.4%) adult clones. The developmental rates of embryos were significantly lower for cell lines expressing EGFP (11.4%) than for non-transfected cells (20.1%, P < 0.05). However, there was no decrease in nuclear transfer (NT) developmental rates (21.5%) when donor nuclei from EGFP transfected cell lines not expressing EGFP but retaining neomycin-resistance gene expression were used as donor nuclei. The NT embryos from transfected and non-transfected cell lines had similar morphology and cell numbers. The results indicated that adult cells can complete clonal propagation including transfection and selection and can be used to produce transgenic NT embryos.
Serological And Haematological Investigations On Bovine Leukaemia Virus Infections In Kars District, Sali̇h Otlu, Fuat Aydin, Oktay Genç, M. Ali̇ Güler, Gürbüz Gökçe
Serological And Haematological Investigations On Bovine Leukaemia Virus Infections In Kars District, Sali̇h Otlu, Fuat Aydin, Oktay Genç, M. Ali̇ Güler, Gürbüz Gökçe
Turkish Journal of Veterinary & Animal Sciences
This study was carried out in order to serologically and haematologically determine the prevalance of Bovine Leukaemia Virus infection in cattle raised in Kars and environs.For this purpose a total of 443 blood samples and 383 milk samples belonging to the various breeds (Zavot, Holstein, Brown-Swiss and Native), and sexes (383 female, 60 male)were examined serologically (Agar Gel Immunodiffusion (AGID)and Enzyme Linked Immunosorbent Assay (ELISA))and haematologically (lymphocyt counts, total leucocyt counts and haematocrit value). All of the 443 blood samples and 383 milk samples examined serologically by AGID and ELISA tests were found to be negative in EBL.As a result …
Changes In The Size Distribution Of Steroidogenic And Non-Steroidogenic Bovine Luteal Cells During The Oestrus Cycle, Arzu Yi̇ği̇t, Şevket Arikan
Changes In The Size Distribution Of Steroidogenic And Non-Steroidogenic Bovine Luteal Cells During The Oestrus Cycle, Arzu Yi̇ği̇t, Şevket Arikan
Turkish Journal of Veterinary & Animal Sciences
The aim of this study was to investigate the size distribution of steroidogenic and non-steroidogenic luteal cells throughout the oestrus cycle. Twelve corpora lutea collected from different stages of the oestrus cycle were used. Corpora lutea collected from nonpregnant cows were dissociated into single-cell suspensions by enzyme treatments. Cells were stained for 3\beta-hydroxysteroid dehydrogenase (3\beta-HSD) activity, a marker for steroidogenic cells. The sizes of 3b-HSD positive (steroidogenic) and negative cells (nonsteroidogenic) were measured with an ocular micrometer. Very small cells (
Effects Of Different Maturation Periods On In Vitro Maturation Of Bovine Oocytes, Sema Bi̇rler, Serhat Pabuççuoğlu, İ. Kamuran İleri̇, Serhat Alkan, Mithat Evecen
Effects Of Different Maturation Periods On In Vitro Maturation Of Bovine Oocytes, Sema Bi̇rler, Serhat Pabuççuoğlu, İ. Kamuran İleri̇, Serhat Alkan, Mithat Evecen
Turkish Journal of Veterinary & Animal Sciences
Primer oocytes (n=157) collected from ovaries of slaughtered cows were matured at 39°C with 95-100% humidity and under a gas mixture of 5% O 2 , 5% CO 2 and 90% N 2 for 22 (n=52), 24 (n=52) and 26 (n=53) hours. Modified Parker's Medium (MPM) supplemented with FSH and 20% ECS was used as maturation medium. At the end of these periods all oocytes were fixated in ethanol:acetic acid in the ratio of 3:1 for 24 hours and stained by %2 aceto-orcein to evaluate the maturational criteria. At the 22, 24 and 26-hours maturation groups, 23(%44.2), 27 (%51.9) and …