Medicine and Health Sciences Commons^™

The Influence Of Fatigue Load To Various Adhesive Resin Luting Agents In A Zirconia-Reinforced Lithium Silicate Ceramic Bonded To Dentin, Ratikorn Watananiyom

Chulalongkorn University Theses and Dissertations (Chula ETD)

Purpose. To examine fatigue failure load value of etch-and-rinse and self-adhesive luting systems used to bond ZLS to dentin. Moreover, this study seeks to evaluate whether the application of unfilled resin on silanated ceramic intaglio surface could improve fatigue failure load value. Methods. Vita Suprinity (VS, Vita Zahnfabrik) blocks were sectioned into cylindrical shape (5 mm in diameter and 1.5 mm in height). All VS were crystallized, and bonded surfaces were treated as followed: Heliobond (HB, Ivoclar Vivadent) application after silanization and non-application of HB. Each VS was cemented to each flat occlusal dentin surface of extracted human molar, following …

The Stains Of Various Lipsticks On Maxillary Anterior Ceramic Veneers, Ratirat Chotipanvidhayakul

Chulalongkorn University Theses and Dissertations (Chula ETD)

This clinical study aims to determine differences in lipstick stains between ceramic veneers and natural teeth. Types of lipsticks affected lipstick stains on ceramic veneers and natural teeth were also investigated. A total of 30 patients were divided into two groups: ceramic-veneer (CV) group and natural-teeth (NT) group with 15 patients in each group. Seven lipstick types: Gloss & Balm, Gloss & Sheer, Cream, Liquid matte, Matte & Frost, Satin, and Matte were applied on patients’ lips. Six labial surfaces of maxillary anterior teeth were evaluated for the frequency of lipstick staining with each type of lipstick. Thus, 90 maxillary …

Prevalence Of Enterococcus Faecalis And Candida Albicans In Teeth Requiring Endodontic Retreatment, Sopida Thipung

Chulalongkorn University Theses and Dissertations (Chula ETD)

Endodontic failures are mainly caused by persistence of microorganisms in the root canal system. Enterococcus faecalis has been reported as the most commonly isolated species from root canals with endodontic treatment failure in many studies, but was not the dominant species in others. Recent studies showed that Candida albicans is found together with Enterococcus faecalis within infected root canals in a polymicrobial community and both species can form biofilm. Therefore, this study aims to investigate the prevalence of Enterococcus faecalis and/or Candida albicans in root canals requiring retreatment by using both culture-based and polymerase chain reaction (PCR) techniques, and examine …

Comparing Patient-Reported Outcome Measures Among 3 Methods Of Dental Implant Placement, Sunida Engkawong

Chulalongkorn University Theses and Dissertations (Chula ETD)

The purpose of this study was to compare patient-reported outcome measures including post-operative pain, swelling, patient's expectation and satisfaction among 3 techniques of dental implant placement including a) conventional freehand, b) dynamic, and c) static Computer-Aided Implant Surgery. Ninety patients were randomly assigned to receive dental implant placement with one of the 3 protocols. Participants were asked to fill in a series of self-administered questionnaires assessing 1) pre-operative expectations, 2) post-operative healing events during the first week after surgery, and 3) overall satisfaction with the procedures at two weeks. Eighty-eight patients completed the study, 2 patients in dynamic CAIS group …

Effect Of Endothelin-1 On Cyclooxygenase-2 Gene Expression And Prostaglandin E2 Synthesis In Human Periodontal Fibroblasts, Supachai Unmanatakoon

Chulalongkorn University Theses and Dissertations (Chula ETD)

The objective of this study is to clarify the effect of endothelin-1 on COX-2 mRNA expression and PGE2 production from human periodontal ligament fibroblasts. Effect of various concentration of ET-1 on cell viability of primary human periodontal ligament fibroblasts was determined by an MTT assay. Then, primary human periodontal ligament fibroblasts were treated with 10 pM ET-1 for 0.5, 1, 2, 4 hours to investigate the time-course effect. Furthermore, to investigate the dose-response effect, periodontal ligament fibroblasts were treated with 1, 10, 100 pM ET-1 for 1 hour. The untreated cells were set as control. RNA extraction followed by cDNA …

Assessing Treatment Outcomes From The Postgraduate Orthodontic Program, Chulalongkorn University, Tanyapak Kongboonvijit

Chulalongkorn University Theses and Dissertations (Chula ETD)

Objective: To evaluate the quality of orthodontic treatment outcomes by using Peer Assessment Rating index (PAR), American Board of Orthodontics Objective Grading System (ABO-OGS) and lateral cephalometric analysis in the cases completed from the postgraduate orthodontic clinic, Chulalongkorn University. And to determine whether any contributing factors correlate with the orthodontic treatment outcomes. Materials and Methods: 100 patients who had completed treatment since 2017 were included in this study. Inclusion criteria included patients with full upper and lower edgewise appliances and completed treatment records. Exclusion criteria included patients with craniofacial syndromes or debond before treatment completion. One calibrated examiner assessed DI, …

Retention Force And Wear Characteristic Of Ball And O-Ring Attachment In Chulalongkorn Mini-Implant Overdenture System., Thanakorn Thimkam

Chulalongkorn University Theses and Dissertations (Chula ETD)

The purpose of this experimental study was to evaluate wear characteristics and retention force of the RetenDent mini-implant overdenture system (Chulalongkorn product) after the insertion-removal fatigue test. One-piece mini-implants attachment system for overdenture, Osstem MS denture® type implant (OSSTEM, Germany GmbH), and RetenDent mini-implant were tested. All samples were subjected to repeated insertion and removal fatigue cycles by the universal testing machine (E1000, INSTRON Instrument, England). Subjected fatigue cycles were 5500 with 1.00Hz frequency to mimic a 5-year insertion and removal three times per day. The retention force was measured by separating the O-ring from the abutment and recorded with …

Influence Of Demineralized Dentin On Marginal Seal Using Primerless Wet Bonding Technique: In-Vivo Study, Thanyarat Lerttriphob

Chulalongkorn University Theses and Dissertations (Chula ETD)

The purpose of this study was to compare microleakage distance between Class V restorations and tooth interface when using either 4-META-MMA/TBB or MMA/TBB resin adhesives by using 1 % ferric chloride in 1% citric acid (1-1) for conditioning periods of 10s (1-1-10s) 30s (1-1-30s) and 60s (1-1-60s) for in-vivo study. Material and methods: A total of 60 Class V box cavities were divided into 6 groups of 10 specimens (n= 10) on the caries-free, vital, and hopeless periodontally-compromised teeth, scheduled for extraction. A Class V cavity (3mm × 3mm × 1.5mm) was prepared on the buccal or lingual surface located …

Microrna Profiles Associated With Bone Healing Of The Extraction Socket In Bisphosphonate Related Osteonecrosis Of The Jaw In Rat Model, Thapakorn Surajkulwatana

Chulalongkorn University Theses and Dissertations (Chula ETD)

Bisphoshonates (BPs) are widely used for treating osteoporosis, multiple myeloma, breast cancer, and bone metastasis cancer. Trauma or tooth extraction in patients treated with BPs can lead to MRONJ development because of its mechanism of action by which interferes bone homeostasis and angiogenesis. Once MRONJ has occurred, this hard-to-cure disease posed the risk of having poor quality of life to the patients. The application of miRNA for curing the diseases is now being of interest in medical field. Aiming at elucidating the role of miRNAs in the pathogenesis of MRONJ, a rat model was used in creating MRONJ and the …

The Overall Esthetic Assessment Of Anterior Single-Tooth Implant Restoration Using Esthetic Sustainable Criteria In 5-Year Clinical Cross-Sectional Study, Tharitsawan Sangprasertkul

Chulalongkorn University Theses and Dissertations (Chula ETD)

Objective: To compare the esthetic score (using the Esthetic Sustainable Criteria) among titanium, zirconia and gold alloy abutments of single tooth implant in 5 years follow up. Mateirals and Methods: 24 patients who were treated with single anterior implant at Faculty of Dentistry, Chulalongkorn University were recruited. There were 8 patients for each abutment material. Demographic data, biological parameters such as modified plaque index (mPI), modified sulcus bleeding index (mSBI), probing depth (PD), radiographic images (Periapical films and CBCT images) were collected. The esthetic score (gingival, prosthodontic and bone score) was evaluated according to the Esthetic Sustainable Criteria. The mean …

Comparative Gene Expression Analysis Of Human Dental Pulp Cells From Maxillary And Mandibular Teeth, Thira Faruangsaeng

Chulalongkorn University Theses and Dissertations (Chula ETD)

Objective : Given the anatomical and locational differences between the maxillary and mandibular teeth, it is possible that there are differences in gene expression patterns of dental pulp cells within those teeth. This study evaluates and compares the gene expression profiles of the maxillary and mandibular human teeth with the aim of finding differences in gene expression patterns of dental pulp cells by RNA-Sequencing technique. Methods : The upper and lower premolar and molar teeth obtained from the same participant were employed. Two pairs of opposing premolar teeth and two pairs of opposing molar teeth were isolated, cultured, and subjected …

Effect Of Ultrasonic Treatment On The Transverse Strength Of Relined Denture Base With Autopolymerizing Acrylic Hard Reline Material, Tithiporn Arunwichit

Chulalongkorn University Theses and Dissertations (Chula ETD)

This study evaluated the effect of ultrasonic treatment and surface treatments on transverse strength of denture bases which relined with auto-polymerizing acrylic resin. The study groups, ninety heat-polymerized acrylic resins (64×10×2 mm) were divided by three surface treatments: no surface treatment (N), applied MMA for 180 seconds (MM), applied MF-MA for 15 seconds (MF). They were relined with auto-polymerized acrylic resin to create 64×10×3.3 mm specimens. Then, they were divided further by ultrasonic which were no ultrasonic (X), ultrasonic in water (W), ultrasonic in 30% ethanol (E). As a result, all test groups were classified as NX, NW, NE, MMX, …

Bcl-Xl Expression In Osteoclasts Of Patients With Medication-Related Osteonecrosis Of The Jaw: A Comparison With Osteoradionecrosis And Osteomyelitis, Vy Tran Ngoc Thuy

Chulalongkorn University Theses and Dissertations (Chula ETD)

Objective: The pathological mechanism of medication-related osteonecrosis of the jaw (MRONJ) is still unknown now. Osteoclasts are cells directly influenced by MRONJ, which might be the key mediator of pathological mechanism. This study aimed to evaluate the histological features of MRONJ, investigate the morphology and quantity of osteoclasts in MRONJ as well as expression of Bcl-xl, and compare it with ORN, OM, and normal jaw bone. Methods: In this study, 57 subjects, including patients with MRONJ, osteoradionecrosis of the jaw (ORN), osteomyelitis of the jaw (OM), and normal jaw bone were studied. Hematoxylin and eosin-stained slides of these diagnosed cases …

Effect Of Desensitizing Toothpaste Containing Calcium Sodium Phosphosilicate On Microtensile Bond Strength Of Adhesive Systems, Warin Sittiwaitayaporn

Chulalongkorn University Theses and Dissertations (Chula ETD)

Purpose: To evaluate the effect of a desensitizing toothpaste, containing calcium sodium phosphosilicate, on microtensile bond strength of adhesive systems treated to dentine. Methods: Fifty-two human third molars were embedded into acrylic resin, and cut to expose flat dentin surface. The specimens were randomly divided into two groups, 1) no brushing, and 2) brushing with Sensodyne Repair&Protect (GSK, London, UK) for 10,000 cycles with a V-8 cross brushing machine (Sabri Dental Enterprise, Inc., USA). Subsequently, both groups were divided into three groups for resin composite build-up using different adhesive agents (OptiBond FL® (Kerr, Orange, CA, USA), Clearfil SE Bond® (Kuraray …

Exploring The Use Of Pulsed Erbium Lasers To Retrieve A Zirconia Crown From A Zirconia Implant Abutment, Ahmed Elkharashi

Theses and Dissertations

Removal of zirconia crowns, commonly used as cement-retained implant fixed restorations, can be challenging. Conventional methods of crown removal are time consuming and often leave irreparable damage to the crown, which can be costly to patients and practitioners. This research explored the use of two different types of pulsed erbium lasers, erbium-doped yttrium aluminum garnet laser (Er:YAG) and erbium, chromium-doped yttrium, scandium, gallium and garnet (Er,Cr:YSGG), as non-invasive tools to retrieve cemented zirconia crowns from zirconia implant abutments. Times needed to remove the crowns were recorded and analyzed using ANOVA (��=0.05). No statistical differences between the debond times of each …

Comparison Of Endodontic Treatment Planning Decisions Using Cone Beam Computed Tomography Versus Periapical Radiography In Previously Endodontically Treated Molars, Sarah Alkanderi

Theses and Dissertations

Objectives: The aim of this study was to determine if treatment plans differ when evaluating previously endodontically treated molars using periapical images (PA’s) alone versus PA’s and CBCT.

Methods: A retrospective cohort chart review was conducted including 67 previously treated molars that had a CBCT taken. Two calibrated board-certified endodontists evaluated standard PA’s. A data sheet questionnaire regarding radiographic findings and treatment plan decisions was completed. Two weeks later, the same steps were repeated but the examiners evaluated both PA’s and CBCT images and again arrived at a recommended treatment plan.

Results: Agreement between raters was assessed with Cohen’s Kappa …

Characteristics Of Alveolar Bone Marrow Cells From Patients Undergoing Dental Extractions Or Dental Implant Therapy, Meng H. Lee

Theses and Dissertations

Alveolar bone marrow stromal cells (aBMSCs) play important roles in craniofacial wound healing. To establish an easy, efficient and reliable method to harvest aBMSCs, we compared three different methods: extraction socket aspiration, osteotomy aspiration and bone core digestion. Samples of aBMSC were collected from two groups of subjects. Group 1 (dental extraction): after dental extraction, 22.5-gauge needles were used to collect 0.5-1cc marrow aspirate. Group 2 (dental implant): during implant surgeries, bone core and 0.5-1cc marrow aspirate were obtained from the osteotomy. Samples were cultured in petri dishes and attached cells were expanded. The population doubling time (PDT), surface markers, …

The Effect Of Print Angulation On The Accuracy And Precision Of 3d-Printed Orthodontic Retainers, Allison Williams

Theses and Dissertations

Purpose: The aims of this study were: 1) to compare the accuracy and precision of 3-

dimensional (3D) printed retainers at various angulations, 2) to evaluate the effect of angulation

on printing time and the amount of resin consumed.

Methods: Using a stereolithography (SLA) 3D printer, a total of 60 clear retainers were printed at five angulations (n=12, each): 15, 30, 45, 60, and 90 degrees. Six retainers were printed each cycle at a random order for all print angulations as print 1 and print 2. Digital images of the original and printed retainers were superimposed. Discrepancies on eight landmarks …

Achieving Dentin Conservation Using Dynamic Navigation Technology For Locating Calcified Canals, Madison W. Saunders

Theses and Dissertations

Purpose: Pulp canal obliteration (PCO) is a common sequela of dental trauma, caries, restorations and vital pulp therapy procedures. Despite the application of high magnification and CBCT imaging, access cavity preparation for such cases is prone to procedural errors that may lead to substantial loss of dentin structure thereby reducing the long-term prognosis. This study aimed to achieve dentin conservation using a novel computer assisted dynamic navigation system (Navident) in comparison to freehand access preparation for locating calcified canals.

Methods: Forty maxillary and mandibular central incisors (tooth #9 and tooth #25) were 3-D printed to simulate PCO. They were randomly …

Amixicile As A Novel Antimicrobial Treatment For Periodontitis: A Pilot Study In The Non-Human Primate, Macaca Mulatta, Denver J. Lyons

Theses and Dissertations

Periodontitis is an inflammatory disease with a bacterial etiology in a susceptible host. Given the bacterial etiology, a selective antimicrobial agent with minimal side effects could be a useful adjunct to traditional therapy. Amixicile is a novel antimicrobial that targets the pyruvate: ferredoxin oxidoreductase (PFOR) which is an enzyme that is critical for anaerobic bacterial metabolism. It has been found to have no effect on commensal, aerobic microbes and has little to no side-effects thus far in animal models. In this study, two nonhuman primates of the Macaca mulatta species with naturally occurring mild chronic periodontitis were studied before and …

Repeat Full Mouth Dental Rehabilitation Under General Anesthesia For Medicaid Eligible Healthy Children Who Are High Caries Risk, Lajoi E. Wiggins

Theses and Dissertations

Purpose: To evaluate the treatment of healthy children who are high caries risk and of low socioeconomic status and who require repeat full mouth dental rehabilitation (FMDR) under general anesthesia (GA).

Methods: A retrospective chart review was performed on healthy Medicaid eligible patients who were initially between 18 months - 6 years of age who underwent FMDR under GA in the OR more than once at the Virginia Commonwealth University (VCU) School of Dentistry, Department of Pediatric Dentistry from January 2008 to December 2018. Biographical information for each patient was gathered into a REDCap™ Survey. Statistical analysis, chi-squared test with …

A Comparison In Pediatric Dental Website Design From A Guardian And Pediatric Dentists’ Perspective., David M. Voth

Theses and Dissertations

Purpose: This project aims to determine which aspects of pediatric dental practice websites are preferable to the guardians of pediatric dental patients and which of these aspects are currently available to guardians on the websites of pediatric dental practices across the United States. The comparison will reveal if practice websites are meeting the needs of the guardians of pediatric dental patients. Our hope is to provide meaningful guidance to pediatric dentists designing new practice websites and to provide the guardians of pediatric dental patients with an appealing online experience during their use of the aforementioned websites. Methods: A survey with …

Using Motivational Interviewing And Technology To Increase Confidence In Nutritional Counseling Among Dental Hygienists: A Thesis, Heather Anderson

EWU Masters Thesis Collection

Purpose: Research shows definite connections between food choices and caries, periodontal disease, and oral cancer. Dental hygienists have the unique opportunity to educate patients on how nutrition can improve oral health. Dental hygiene students are introduced to these concepts but struggle to gain confidence to share this knowledge with patients. This study looked at an educational module on motivational interviewing (MI) and an electronic assessment tool to build student confidence with nutritional counseling.

Methods: Students participated in an educational module to review MI and introduce the electronic nutritional risk assessment and counseling tool. Prior to the module, participants completed a …

Accuracy Of Blx And Blt Guided Implants In The Edentulous Maxilla: An In Vivo Study, Benjamin Andrew Kordusky

Graduate Theses, Dissertations, and Problem Reports (ETD)

Objectives: To determine the accuracy of dental implants placed using a dual scan CBCT protocol with a SLA 3D printed mucosa supported surgical guide using a flapless surgical approach with regard to sleeve position, type of implant, and regional location in the maxillary arch.

Methods: Nine patients received 4 dental implants utilizing a fully guided, flapless approach with a mucosa supported SLA surgical guide with 3 fixation pins. Implants were immediately loaded using an attachment placed in an existing Maxillary complete denture. Accuracy of implant positions were evaluated by the Treatment Evaluation module of coDiagnostiX (DentalWings, Montreal, Canada). Statistical analysis …

Fracture Resistance Of Simulated External Cervical Resorption In Anterior Teeth Restored With And Without A Fiber Post., Michelle M. Romeo

Graduate Theses, Dissertations, and Problem Reports (ETD)

Introduction: External cervical resorption (ECR) is a destructive type of external resorption that can be difficult to treat. ECR usually begins subgingival at the cementoenamel junction of teeth and continues apically and circumferentially. This substantial loss of tooth structure may make these teeth more prone to fracture and should be addressed when treatment planning teeth with ECR. The aim of this in vitro study was to evaluate the fracture resistance of treated and non-treated teeth with simulated ECR using two different treatment modalities.

Materials and Methods: Forty-eight extracted human permanent mandibular central incisors were selected. Teeth were randomly divided into …

The Visual And Spectrophotometric Effect Of External Bleaching On Omnichroma Resin Composite And Natural Teeth, Michael Brian Evans

Graduate Theses, Dissertations, and Problem Reports (ETD)

Objective: To determine the visual and spectrophotometric effect external tooth bleaching has on natural tooth structure and OMNiCHROMA composite at various number of treatments. Variables were compared to determine if there is a detectable difference between the composite and the tooth before and after bleaching.

Methods: Preparation and restoration of 25 previously extracted teeth was completed. OMNiCHROMA composite was used. Each tooth was measured visually with a VITA shade guide and a colorimeter. Measurements were recorded before bleaching, after 5 and 10 treatments. Statistical analysis was completed and ΔE values between the composite and tooth were compared between the 3 …

Tobacco Cessation In A Dental School Setting, Kerri T. Simpson

Graduate Theses, Dissertations, and Problem Reports (ETD)

Introduction

Tobacco use remains the single largest preventable cause of death in the United States. Currently, the prevalence of tobacco use is 20.7% in adults (≥18 years old) and 31.2% among high school students. According to various studies, over 60% of Americans want to quit tobacco, but it takes a person several attempts to quit before they are successful. There is limited data in the literature that discusses the effectiveness of tobacco cessation programs in US dental schools. Therefore, information on the effectiveness of a tobacco cessation program in a dental school setting could benefit this state and the problem …

Comparison Of Skeletal And Dentoalveolar Changes Between Two Bone-Borne Maxillary Expanders, Mohamad Sarraj

Graduate Theses, Dissertations, and Problem Reports (ETD)

Introduction: Bone-borne maxillary expander (BBME) are designed to provide additional anchorage to widen the maxillary arch for patients who exhibit maxillary transverse deficiency. One form of BBME is the hybrid BBME which uses mini-implants (bone-borne) and posterior teeth (tooth-borne) as anchorage for maxillary expansion. The other form of BBME is absolute BBME which only uses mini-implants as anchorage. It is not clear in the literature if there is a difference in skeletal and dental changes with these two types of expanders. The objective of this study was to compare the skeletal, dentoalveolar, and periodontal changes between the hybrid and the …

The Pattern Of Odontogenic Referred Pain, James J. Poulos

Graduate Theses, Dissertations, and Problem Reports (ETD)

Background: Referred odontogenic pain (RP) is a phenomenon that is seen regularly in dentistry and it is not extensively understood. It can make formulating a proper diagnosis difficult and its presence can lead to inappropriate treatment.

Methods: 250 patients experiencing odontogenic pain within the dental clinics of West Virginia University were surveyed. The survey inquired about the patient’s biographical information, medical history, characteristics of the pain, the presence and location of RP, and the psychosocial effects of the pain. Additionally, the endodontic diagnosis of the offending tooth was determined by a member of the endodontic department. The collected …

Retrospective Evaluation Of The Changes In The Nasal And Pharyngeal Airway Volume After Miniscrew Assisted Rapid Palatal Expansion (Marpe) Appliance, Joanna Song

Graduate Theses, Dissertations, and Problem Reports (ETD)

Background and Objectives: A transverse deficiency of the maxilla is a pervasive skeletal problem that can affect almost a quarter of the primary dentition population. The interesting relationship between rapid maxillary expansion (RME) and its effects on the surrounding airway is not well described in the literature. For today’s contemporary orthodontist, there are a variety of rapid palatal expanders (RPE) and expansion protocols to prescribe for patient treatment. Of late, the miniscrew-assisted RPE (MARPE) has been receiving attention by clinicians because of its increased orthopedic effects when compared to conventional RPE while avoiding the surgical procedure needed with SARPE. …