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Full-Text Articles in Systems Biology
Structure-Based Design Of Hepatitis C Virus Vaccines That Elicit Neutralizing Antibody Responses To A Conserved Epitope, Brian G. Pierce, Elisabeth N. Boucher, Kurt H. Piepenbrink, Ejemel Monir, Chelsea A. Rapp, William D. Thomas Jr., Eric J. Sundberg, Zhiping Weng, Yan Wang
Structure-Based Design Of Hepatitis C Virus Vaccines That Elicit Neutralizing Antibody Responses To A Conserved Epitope, Brian G. Pierce, Elisabeth N. Boucher, Kurt H. Piepenbrink, Ejemel Monir, Chelsea A. Rapp, William D. Thomas Jr., Eric J. Sundberg, Zhiping Weng, Yan Wang
Kurt Piepenbrink
Despite recent advances in therapeutic options, hepatitis C virus (HCV) remains a severe global disease burden, and a vaccine can substantially reduce its incidence. Due to its extremely high sequence variability, HCV can readily escape the immune response; thus, an effective vaccine must target conserved, functionally important epitopes. Using the structure of a broadly neutralizing antibody in complex with a conserved linear epitope from the HCV E2 envelope glycoprotein (residues 412 to 423; epitope I), we performed structure-based design of immunogens to induce antibody responses to this epitope. This resulted in epitope-based immunogens based on a cyclic defensin protein, as …
Strand-Specific Libraries For High Throughput Rna Sequencing (Rna-Seq) Prepared Without Poly(A) Selection, Zhao Zhang, William E. Theurkauf, Zhiping Weng, Phillip D. Zamore
Strand-Specific Libraries For High Throughput Rna Sequencing (Rna-Seq) Prepared Without Poly(A) Selection, Zhao Zhang, William E. Theurkauf, Zhiping Weng, Phillip D. Zamore
Zhao Zhang
BACKGROUND: High throughput DNA sequencing technology has enabled quantification of all the RNAs in a cell or tissue, a method widely known as RNA sequencing (RNA-Seq). However, non-coding RNAs such as rRNA are highly abundant and can consume >70% of sequencing reads. A common approach is to extract only polyadenylated mRNA; however, such approaches are blind to RNAs with short or no poly(A) tails, leading to an incomplete view of the transcriptome. Another challenge of preparing RNA-Seq libraries is to preserve the strand information of the RNAs. DESIGN: Here, we describe a procedure for preparing RNA-Seq libraries from 1 to …
Immunopurification Of Ago1 Mirnps Selects For A Distinct Class Of Microrna Targets, Xin Hong, Molly Hammell, Victor Ambros, Stephen Cohen
Immunopurification Of Ago1 Mirnps Selects For A Distinct Class Of Microrna Targets, Xin Hong, Molly Hammell, Victor Ambros, Stephen Cohen
Victor R. Ambros
microRNAs comprise a few percent of animal genes and have been recognized as important regulators of a diverse range of biological processes. Understanding the biological functions of miRNAs requires effective means to identify their targets. Combined efforts from computational prediction, miRNA over-expression or depletion, and biochemical purification have identified thousands of potential miRNA-target pairs in cells and organisms. Complementarity to the miRNA seed sequence appears to be a common principle in target recognition. Other features, including miRNA-target duplex stability, binding site accessibility, and local UTR structure might affect target recognition. Yet computational approaches using such contextual features have yielded largely …
List Of Thylakoid Zinc-Finger Proteins In Arabidopsis, Yan Lu
List Of Thylakoid Zinc-Finger Proteins In Arabidopsis, Yan Lu
Yan Lu
No abstract provided.
The Occurrence Of A Thylakoid-Localized Small Zinc Finger Protein In Land Plants, Yan Lu
The Occurrence Of A Thylakoid-Localized Small Zinc Finger Protein In Land Plants, Yan Lu
Yan Lu
No abstract provided.
A Small Zinc Finger Thylakoid Protein Plays A Role In Maintenance Of Photosystem Ii In Arabidopsis Thaliana, Yan Lu, David Hall, Robert Last
A Small Zinc Finger Thylakoid Protein Plays A Role In Maintenance Of Photosystem Ii In Arabidopsis Thaliana, Yan Lu, David Hall, Robert Last
Yan Lu
No abstract provided.
Chloroplast 2010: A Database For Large-Scale Phenotypic Screening Of Arabidopsis Mutants, Yan Lu, Linda Savage, Matthew Larson, Curtis Wilkerson, Robert Last
Chloroplast 2010: A Database For Large-Scale Phenotypic Screening Of Arabidopsis Mutants, Yan Lu, Linda Savage, Matthew Larson, Curtis Wilkerson, Robert Last
Yan Lu
No abstract provided.
Chloroplast Phenomics: Systematic Phenotypic Screening Of Chloroplast Protein Mutants In Arabidopsis, Yan Lu, Linda Savage, Robert Last
Chloroplast Phenomics: Systematic Phenotypic Screening Of Chloroplast Protein Mutants In Arabidopsis, Yan Lu, Linda Savage, Robert Last
Yan Lu
No abstract provided.
Large Scale Reverse Genetics In Arabidopsis: Case Studies From The Chloroplast 2010 Project, Imad Ajjawi, Yan Lu, Linda Savage, Shannon Bell, Robert Last
Large Scale Reverse Genetics In Arabidopsis: Case Studies From The Chloroplast 2010 Project, Imad Ajjawi, Yan Lu, Linda Savage, Shannon Bell, Robert Last
Yan Lu
No abstract provided.
Web-Based Arabidopsis Functional And Structural Genomics Resources, Yan Lu, Robert Last
Web-Based Arabidopsis Functional And Structural Genomics Resources, Yan Lu, Robert Last
Yan Lu
No abstract provided.
New Connections Across Pathways And Cellular Processes: Industrialized Mutant Screening Reveals Novel Associations Between Diverse Phenotypes In Arabidopsis, Yan Lu, Linda Savage, Imad Ajjawi, Kathreen Imre, David Yoder, Christoph Benning, Dean Dellapenna, John Ohlrogge, Katherine Osteryoung, Andreas Weber, Curtis Wilkerson, Robert Last
New Connections Across Pathways And Cellular Processes: Industrialized Mutant Screening Reveals Novel Associations Between Diverse Phenotypes In Arabidopsis, Yan Lu, Linda Savage, Imad Ajjawi, Kathreen Imre, David Yoder, Christoph Benning, Dean Dellapenna, John Ohlrogge, Katherine Osteryoung, Andreas Weber, Curtis Wilkerson, Robert Last
Yan Lu
No abstract provided.
The Importance Of Maltose In Transitory Starch Breakdown, Yan Lu, Thomas Sharkey
The Importance Of Maltose In Transitory Starch Breakdown, Yan Lu, Thomas Sharkey
Yan Lu
No abstract provided.
Cellular And Organ Level Localization Of Maltose In Maltose-Excess Arabidopsis Mutants, Yan Lu, Jon Steichen, Sean Weise, Thomas Sharkey
Cellular And Organ Level Localization Of Maltose In Maltose-Excess Arabidopsis Mutants, Yan Lu, Jon Steichen, Sean Weise, Thomas Sharkey
Yan Lu
No abstract provided.
The Role Of Cytosolic A-Glucan Phosphorylase In Maltose Metabolism And The Comparison Of Amylomaltose In Arabidopsis And E. Coli, Yan Lu, Jon Steichen, Jian Yao, Thomas Sharkey
The Role Of Cytosolic A-Glucan Phosphorylase In Maltose Metabolism And The Comparison Of Amylomaltose In Arabidopsis And E. Coli, Yan Lu, Jon Steichen, Jian Yao, Thomas Sharkey
Yan Lu
No abstract provided.
The Role Of Amylomaltase In Maltose Metabolism In The Cytosol Of Photosynthetic Cells, Yan Lu, Thomas Sharkey
The Role Of Amylomaltase In Maltose Metabolism In The Cytosol Of Photosynthetic Cells, Yan Lu, Thomas Sharkey
Yan Lu
No abstract provided.