Research Methods in Life Sciences Commons^™

Flow Cytometry For Identification Of Prdm1-Eyfp Transgenic Mice, Kayla M. Hufford, Olyvia D. Weaver, Nicole M. Young, Aspen E. King, Gregory K. Dekrey

Ursidae: The Undergraduate Research Journal at the University of Northern Colorado

The goal of this study was to determine if flow cytometry could be used to identify transgene carrier mice [B6.Cg-Tg(Prdm1-EYFP)1Mnz/J] by their expression of the enhanced yellow fluorescent protein (eYFP) in peripheral blood lymphocytes. In these mice, eYFP expression is under the control of the Prdm1 gene promoter. Littermates were identified as being either wild type or transgene carriers using a polymerase chain reaction (PCR) analysis of tail tissue. Peripheral blood leukocytes were isolated from each of the mice, and both the percent and mean fluorescence intensity (MFI) were determined for eYFP expression by lymphocytes. We found that blood lymphocytes …

A Qualitative Study On The Factors Affecting The Willingness Of 3rd Level Students To Register As Organ Donors In Ireland, Evelyn Hayes, Erika Horan, Cian Lally, Aisling Murphy

International Undergraduate Journal of Health Sciences

A wide range of factors contribute to an individual’s choice whether or not to register as an organ donor. The knowledge of the Irish population at large around the area of donation and transplantation is varied. A research survey was designed to be completed by third level students. The purpose of the research was to determine the most important factors that played a role in their decisions regarding opting-in to organ donation. A second aim of the survey was to determine the participants’ levels of knowledge and understanding on the ‘opt in’ donation system in place here in Ireland.

The …

Full Issue: The International Undergraduate Journal Of Health Sciences, Volume 1, Issue 1, June 2021

International Undergraduate Journal of Health Sciences

The full June 2021 issue (Volume 1, Issue 1) of the International Undergraduate Journal of Health Sciences

How A Low-Cost Method For Cumulative Water-Sampling Shows Need For Improvement Of Legal Public-Contact Standards In The United States, Samuel C. Kessler

Grawemeyer Colloquium Papers

Across the world, it is estimated that 4.5 billion people live near water sources “impaired” for use or contact. Standards for human-interaction are established by international organizations such as the WHO, and legislative bodies from national to local levels with jurisdiction over the quality of our waterways to ensure public & environmental health. Standards are often assessed from “grab-samples” taken from a waterbody at a certain time, with a minimum number analyzed. Water-quality standards in the United States are enforced under the Clean Water Act (CWA) via the Environmental Protection Agency (EPA), applying to “waters of the United States” (WOTUS). …

A Decision Tool To Identify Population Management Strategies For Common Ravens And Other Avian Predators, Andrea F. Currylow, Brenda J. Hanley, Kerry L. Holcomb, Timothy Shields, Stephen Boland, William I. Boarman, Mercy Vaughn

Human–Wildlife Interactions

Some avian species have developed the capacity to leverage resource subsidies associated with human manipulated landscapes to increase population densities in habitats with naturally low carrying capacities. Elevated corvid densities and new territory establishment have led to an unsustainable increase in depredation pressure on sympatric native wildlife prey populations as well as in crop damage. Yet, subsidized predator removal programs aimed at reducing densities are likely most effective longer-term when conducted in tandem with subsidy control, habitat management, and robust assessment monitoring programs. We developed decision support software that leverages stage structured Lefkovitch population matrices to compare and identify treatment …

A Zugunruhe Data Collection System Using Passive Infrared Sensors, Ryan Terry, Luis E. Ramirez, Carol Carrera, Abigail Kimmitt, Kira Delmore, Ana Goulart

Pursue: Undergraduate Research Journal

When engineers and biologists work together, there is a lot to learn on both sides. For instance, our work introduced us to zugunruhe, which is a German word that means “unrest”. It is used in the context of migratory birds, as they become restless at night, inside their cages, during their migratory period. When does zugunruhe start? It usually starts when the weather becomes cold and the days shorter, but it varies for different bird species. Moreover, global warming has caused changes in zungunruhe’s timing, which made it even harder to predict. Another question is about genetics: is …