Research Methods in Life Sciences Commons^™

Repair Of Lethal Damage Induced In A Hamster X Amphibian Hybrid Tissue Culture Cell Line By Ultraviolet Light And Gamma Ray, Susan Kulp, Ann Page, H. Gaston Griggs

Journal of the Arkansas Academy of Science

A hybrid cell line was formed by fusing cells from two established vertebrate tissue culture cell lines (V79B1 hamster and A84 Xenopus), which were known to differ substantially in resistance to some radiation-induced lesions and in associated repair potential. Ultraviolet and gamma ray dose-survival relations were then determined and analyzed for the three cell lines, primarily to compare the radiosensitivity (Do dose) of the hybrid line with that of each parental line for each radiation. Further experimentation was conducted to determine the extent to which observed differences in these Do doses could be attributed to interactions of the combined parental …