Research Methods in Life Sciences Commons^™

Bryophyta Sensu Stricto Presence And Function In The Epiphytic Ecosystems Of The Tropical Montane Cloud Forests Of El Valle De Anton, Teaghan Duff

Independent Study Project (ISP) Collection

Bryophyta sensu stricto, colloquially known as moss, is often found growing epiphytically in cloud forest ecosystems. Under these conditions, moss may fulfill a secondary foundational species (FS) role, supporting increased habitat for other organisms while remaining dependent on the primary FS host tree. Exploring the dual relationship of moss as both a dependent and supportive organism allows for better understanding of its connective role within ecosystems. Research in the cloud forests of El Valle de Anton focused on relative abundance, environmental surroundings, morphology, and microclimate of mosses to offer baseline data on these connections. Mosses along four different trails within …

Bryophyte (Sl) Growth And Environmental Factors Along An Altitudinal Gradient On Cerro Gaital, El Valle, Coclé, Panamá, Julian Grace-Martin

Independent Study Project (ISP) Collection

Research on bryophytes (SL) in the tropics has been lacking compared with the high number of species found there and the potential benefit of bryophytes (SL) as indicators of pollutants and other forms of human disturbance. This study investigated whether or not bryophyte (SL) growth patterns showed relation when compared with environmental factors on the mountain Cerro Gaital in El Valley, Panamá. Whether or not bryophytes (SL) are present, extent of the area covered by their growth, and height of their growth are all aspects of bryophyte (SL) growth that were used. In this study, these three aspects are compared …

Analyzing Human - Nonhuman Primate Conflict Mitigation Techniques In Mto Wa Mbu, Northern Tanzania, Lily Adams

Independent Study Project (ISP) Collection

Human – non-human primate conflict is particularly crucial due to primates’ high capacity to live among human populations. The study to analyze Human - Nonhuman Primate Conflict Mitigation Techniques, was carried in November 2023 at Mto wa Mbu, Northern Tanzania. To analyze techniques currently being used to mitigate human – non-human primate conflict, this study collected data through semi-structured interviews. Over 87% (n=35) of respondents used multiple mitigation techniques simultaneously. 80% of respondents (n=32) reported using projectiles to ward off foraging primates, 75% of respondents (n=30) reported using loud noises (made either by the voice/body or by manipulating noisemakers), 67.5% …

Abcds Of Triggerfish In Pemba And Misali Island: Abundance, Biodiversity, Behaviour, Cultural Significance And Distribution, Hannah Ng Shueh Yi

Independent Study Project (ISP) Collection

This study takes a socioecological approach to understanding triggerfish (Balistidae) in Pemba Island, using Misali Island reefs as a sample ecological site. Two reef sites on Misali Island and three of four districts in Pemba were observed during the study. The abundance of Balistipus undulatus at one of two study sites is close to the maximum carrying capacity of the reef. Overall observed biodiversity was low. Behavioural trends point to distinct species specific defence strategies and species aggression taking different forms. Triggerfishes cultural significance was primarily as a food fish, though it is not highly sought after. With polarised responses …

Understory Epiphyte Hydrology: Analyzing Water Storage Capacity Of Epiphytes Along An Elevational Gradient In Western Ecuadorian Cloud Forest, Angelina Dodge

Independent Study Project (ISP) Collection

Epiphytes are defined as plants that grow on a host, often another plant, and acquire their nutrients and water from the atmosphere. As such is true, epiphyte composition is largely dependent upon atmospheric and thus climactic conditions. Due to high levels of atmospheric water availability that results from the frequent presence of mist, clouds, and high humidity, epiphytic plants grow and thus store water within the above ground region of the Andean cloud forest at levels higher than in any other ecosystem found in Ecuador. This study was done in hopes of revealing any trends of epiphytic water storage capacity …

A Comparative Plague Study Of Cacao Fungal Disease In Cacao Pods Within Monocultures And Indigenous Agroforests In Ecuador’S Napo Province, Seamus Mccarthy

Independent Study Project (ISP) Collection

This study analyzes the composition of three major fungal diseases in Theobroma cacao fruits compared between monocultures and chakra agroforests in the Napo province of Ecuador with the goal of noting similarities and differences in the disease composition between the two systems, as well as investigate possible variation within this poorly understood category of agroforest to better structure future studies. Cacao pods on sampled trees were counted and fungal infections identified visually and by touch. Chakra systems were selected in the communities of Cinco de Enero and Seis de Marzo to the Southwest of Tena, Ecuador. Monoculture data was collected …

Understanding Invasive Predation: Detections Of Feral Cats (Felis Catus) On Kangaroo Island’S Western River Refuge, Leah Boget

Independent Study Project (ISP) Collection

Feral cats (Felis catus) are a priority-level threat for small to medium-sized native vertebrates across Australia and especially so in insular environments like that of Kangaroo Island, South Australia. The island is home to several endangered and geographically restricted species, including the Kangaroo Island Dunnart (Sminthopsis aitkeni), an endangered marsupial endemic to Kangaroo Island. When bushfires spread across the island and burned x percent of native vegetation in late 2019 to early 2020, these populations were critically impacted and post-fire feral cat predation on the island risked their extinctions, motivating the creation of a refuge with …

Mycorrhizal Fungi And Reforestation In An Eastern Lowland Rainforest Of Madagascar, Sophia Gibby

Independent Study Project (ISP) Collection

Most terrestrial plants worldwide make associations with mycorrhizal fungi, yet the fungal diversity of Madagascar is vastly unknown. This research project is a preliminary investigation into the mycorrhizal status of 19 tree species in and near Ranomafana National Park (RNP), a lowland tropical forest on the southeast coast of Madagascar. Arbuscular mycorrhizal (AM) colonization in these species was studied in root samples under the microscope in the laboratory of Centre ValBio (CVB). The degree of AM infection was assessed for three study areas: one directly adjacent to a protected area and two reforestation sites supported by CVB. For the 19 …

Orb-Weaver Diversity And Niche Partitioning In Ecuador’S Amazonian Foothills: What Spiders Can Reveal About Tree Fall Gaps, Streams, And Cultivated Areas, Riley Endries

Independent Study Project (ISP) Collection

As abundant predators at the top of arthropod food chains, spiders are excellent bioindicators. Araneae is a megadiverse and extremely understudied order, especially in the tropics. This study aims to investigate the diversity of orb-weaver spiders across a disturbance gradient and variety of habitat types as well as their microhabitat preferences and potential niche partitioning. Spider collection was performed on spiders of the families Araneidae, Tetragnathidae, and Theridiosomatidae in Sumak Kawsay in Situ biological reserve in the lower elevation cloud forest of Ecuador’s Andean foothills. Spiders and webs were observed and analyzed from primary and secondary forest tree fall gaps, …

Arthropod Assemblages At The Intersection Of Epiphyte And Soil Habitats: An Assessment Of Understory Level Nonvascular Epiphyte Communities And Their Connectivity Within A Tropical Montane Rainforest, David “Max” Jones

Independent Study Project (ISP) Collection

Canopy arthropods communities represent a disproportionate amount of global species richness (Stuntz 2001). Understanding arthropod composition and connectivity of lower epiphyte communities is important in understanding how canopy arthropods communities are formed and will respond to change (Floren and Linsenmair 1998). As such this study examined arthropod assemblages living within nonvascular epiphyte communities (epiphyte mats) living directly upon lower trunk regions of 24 trees in a tropical montane rain forest within the Santa Lucia Nature Reserve in Pichincha, Ecuador. Trees were assessed for size, epiphyte diversity, and epiphyte coverage before arthropod sampling within the epiphyte mats. Soil assessments were taken …

Tree To Tree: Phylogenetics Of Diverse Anura Using Portable Lab Equipment: Primer Optimization, Bioinformatic Pipeline, And Phylogenetic Analyses Reveal Potential New Species And Ability To Identify Evolutionary Relationships In A Hyper Diverse Anura Clade Using Nanopore Sequencing, Elinor Sterner

Independent Study Project (ISP) Collection

The Corredor Llanganates-Sangay is an extremely significant region for conservation, coined a “gift to the earth” by the World Wildlife Fund. Understanding amphibians species and populations is key due to their roles as hyper sensitive bioindicators. In recent years, portable sequencing equipment (Mini PCR thermocyclers, Oxford Nanopore Technology’s MinION sequencer, etc) make genetic data collection in remote areas possible, greatly increasing potential for conservation genetics and genomics. This study uses portable equipment to sequence two mitochondrial loci in 104 Anuran samples collected in four sites in the Corridor and presents phylogenies of each gene as well as a concatenated phylogeny. …

Observational Study Of Woolly Monkey Behavior And Vocalizations: Behavioral Time Allotment And Vocalization Habits Of A Small Population Of Poeppig’S Woolly Monkey (Lagothrix Lagotricha Poeppigii) In The Ecuadorian Amazon, Marina Smith-Hanke

Independent Study Project (ISP) Collection

Poeppig’s woolly monkey (Lagothrix lagotricha poeppigii), a species of primate native to the Amazonian regions of western Brazil, eastern Ecuador, and northeastern Peru, is classified as endangered by the IUCN. These large, frugivorous primates are threatened by habitat loss and climate change as a result of human interference. Deforestation, hunting, petroleum mining, and illegal trafficking are all huge threats to this species. As a result of these pressures, their populations have decreased by at least 30% over the past three generations. However, as keystone seed dispersers, they are essential for Amazonian ecosystem health; without their seed dispersal services, a loss …

The Nesting Behavior Of Russet-Backed Oropendola On Sumak Allpa, Ecuador: An Analysis Of Nest Construction And Interactions With Competing Species, Claire Molina

Independent Study Project (ISP) Collection

Nests are the literal place of birth of bird species; they are incredibly important in the survival and reproduction of populations. Therefore, nesting behavior is a gateway into the lives and success of the animals. With the degradation of natural landscapes due to human activity, there is more of a need to monitor these nests to ensure healthy inhabitants. The Russet-backed Oropendola (Psarocolius angustifrons) creates unique, pendulous nests that are subject to piracy, parasitism, and predation; as well as weather phenomenon’s such as wind and rainstorms. This study describes the nest construction behavior, nest materials and nest architecture by collecting …

Hummingbird Diversity, Abundance, And Feeding Interactions Across Three Floral Communities On Mount Totumas, Chiriquí, Panamá, Zachary Ginn

Independent Study Project (ISP) Collection

(Trochilidae) are a large Neotropical bird family of nectar-feeders that have evolved as pollinators of many Neotropical plants. Interactions between hummingbirds and plants form mutualistic networks that may change in structure over environmental and anthropogenic gradients. While the unique dynamics of hummingbird diversity and floral interactions have been studied throughout the Neotropics, differing drivers between locations emphasizes the need for further local research. This deficit is especially crucial in biodiverse and understudied locations like the Western Highlands of Panamá. In this study, I investigated how hummingbird diversity, abundance, and floral interactions differed between Cloud Forest, Garden, and Oak Forest on …

Good Cop/Bad Cop: A Comparative Analysis Of Affiliative And Aggressive Behaviors By Adult Female And Male Olive Baboons With Infants At Randilen Wildlife Management Area, Tanzania, Jesse Elop

Independent Study Project (ISP) Collection

This study investigated the infant-directed behaviors of adult male and female olive baboons in Randilen Wildlife Management Area in April of 2022. The objectives were 1) To determine the difference between olive baboon males and females in frequency and duration of listed behaviors towards infants; 2) To classify the interactions of adult male and adult female olive baboons with infants as either primarily affiliative or primarily aggressive; 3) To determine the frequency of selected behaviors at different reproductive phases (lactation, estrus, neutral, indeterminant) amongst female olive baboons towards infants. A single baboon troop in Randilen Wildlife Management Area was observed …

Assessing The Threat Of Coral Decline On Fish Diversity In Response To Temperature, Ryland Talmadge

Independent Study Project (ISP) Collection

Misali, a PECCA-protected island off the east coast of Pemba, Tanzania, is considered to be a marine biodiversity hotspot with a protected Non-Extractive Zone (NEZ) and remaining Extractive Zone (EZ). Upon protection, the island’s fringe reefs faced severe coral bleaching during the 1998 bleaching event, estimated to kill up to 70% of corals. Since the event, several others have occurred in addition to restructuring the management plan in 2006 to expand the region to 1000km. While the damage from the most recent coral bleaching event in 2020 has yet to be surveyed, this study investigates the current reef health in …

The Relationship Between Substrate Composition, Community Structure And Feeding Preferences Of Parrot Fishes (Scaridae) In Anmardub, Guna Yala Coastal Reefs, Caitlin Amman

Independent Study Project (ISP) Collection

Coral reefs are important marine ecosystems, as they support biodiversity and generate buffer zones, yet their abundance is incredibly limited globally. One of the main threats that coral reefs face is excessive macroalgal coverage, which limits sunlight from reaching corals, and thus, limits the energy that can reach them. Parrot fish (Scaridae) are keystone herbivores, as they consume macroalgae growing on corals, which aids in reef survivability. While there is previous research on the abundances, sizes, and feeding habits of Scaridae species in the Caribbean, there is no published research on how community structure and feeding preferences of parrot fishes …

A Quantitative Study Of Orchids And Their Proximate Environments Over An Elevational Gradient On The Northern Slope Of Montagne D’Ambre, Hazel Schrader

Independent Study Project (ISP) Collection

This research investigated change in orchid populations and their proximate environments on the Northern slope of Montagne d’Ambre, a subhumid forest in Northern Madagascar. Orchid density, way of life (epiphyte or terrestrial), phenology, and associations were recorded over 100m elevation gradients and between forest types (primary, secondary, dry, and humid forests at the same altitude). Additionally, orchid preferences for certain heights in the forest and DBH of host trees were analyzed. The establishment of 50X20m plots in 100m elevation gradients combined with ground survey yielded significant results demonstrating change along with elevation as well as forest types. Orchid density increased …

A Ladder Of Endemicity: A Pioneering Study Of Anuran Communities Along An Elevational Gradient In The Eastern Andean Cloud Forests, Ecuador, Ella Halbert

Independent Study Project (ISP) Collection

Per unit of area, Ecuador is home to the most herpetofauna of any region on Earth, yet many ecosystems are under researched. Positioned between the Eastern Andes and the Amazon rainforest, the Río Pastaza Basin of Ecuador has been the recent focus of anuran biodiversity surveys and is home to many endemic species. This study aimed to determine how the anuran community composition of a vertical transect in the Llanganates National Park varied between elevations. A 7-day expedition was conducted along a new trail up Abitagua Range spanning 1,050 m in elevation. Each night, visual encounter surveys were employed to …

A Biological Assessment Of Water Quality In El Placer, Ecuador: The Effect Of Agriculture On Stream Health And The Quality Of Historical Versus Current Drinking Water Sources, Danielle Kleinberg

Independent Study Project (ISP) Collection

Although fresh water is one of Ecuador’s most abundant resources, high quality drinking water for its inhabitants is scarce (Wingfield et al., 2021). The most prevalent sources of water pollution in Ecuador are domestic waste, silver and gold mining, oil production, and agricultural chemicals (Buckalew et al., 1997). El Placer, a village located in Tungurahua, Ecuador, is highly dependent on agriculture as a source of income. The first objective of this study was to determine the effect of agriculture on the El Placer’s Tía Anita Stream through comparing the water quality at three sites with varying agricultural influence. The second …

A Phenological Analysis Of Páramo Vegetation Along An Altitudinal Gradient: The Influence Of Camelids On Andean Flora In The Páramo Of Chimborazo, Ecuador, Tessa Seifried

Independent Study Project (ISP) Collection

This study hopes to reveal the mechanisms behind camelid-plant interactions in the Chimborazo province of Ecuador, which is known for having a large population of domesticated alpacas (Vicugna pacos), along with wild vicuñas (Vicugna vicugna) that were reintroduced into the area 30 years ago. These camelid species, especially vicuñas, are understudied within Ecuador in regards to their effects on plant composition, diversity, and spatial phenology. To gain a better insight into this topic, three sites were chosen: a site inhabited by only vicuña, a site inhabited by only alpaca, and a site inhabited by both species. The vicuña site sat …

Beach Suitability For Nesting Olive Ridley Sea Turtles (Lepidochelys Olivacea), Playa Malena, Azuero Peninsula, Panama, Soleil Foy

Independent Study Project (ISP) Collection

Sea turtle species all over the world are facing continuous declines in their populations partly due to diminished viable nesting habitat. Understanding sea turtle nesting preferences and the suitability of beaches for reproductive success is necessary in order to sustain their populations and ensure their conservation. Olive ridley sea turtles (Lepidochelys olivacea) are one of the species experiencing this trend, especially in the Eastern Pacific. This study examined the beach suitability of a site known as Playa Malena in the Azuero Peninsula in Panama for Olive ridley nesting. This site was compared to the suitability of 7 other …

An Eventful Past Predicting A Bizarre Future: Assessing The Influence Of Ocean Circulation On Key Fish Species In Icelandic Waters. An Analysis Of Past Circulation Records In Order To Predict The State Of Demersal Fish Stocks In Future Climate Scenarios, Matthew Engfer

Independent Study Project (ISP) Collection

Feedback-driven changes in North Atlantic Ocean circulation are affecting marine ecosystems off the coast of Iceland and are predicted to have differing outcomes by 2100. In this study, data reflecting Icelandic fish stock distributions was compiled on a map with oceanographic temperature and depth in order to observe patterns of population changes with changes in ocean currents. After evaluating past temperature effects on demersal fish stocks, future habitat predictions were estimated in weaker ocean circulation scenarios. A number of assumptions were made in order to pinpoint a correlation between ocean temperature and fish habitat. Iceland’s unique location and climate offers …

An Assessment Of Terrestrial Decapoda Diversity Across Three Ecological Zones In Mida Creek, Kenya, Reese Yount

Independent Study Project (ISP) Collection

Mangroves make up one of the most effective natural remedies at combating climate change today. They represent great commercial interest worldwide and yet, are being degraded at an unsustainable rate. If successful mangrove conservation plans are to be implemented for our posterity, mangrove ecosystems need to be better understood at the community level. Mangrove crabs make up the most diverse and populace mangrove inhabitants. They are classified as ecosystem engineers and their potential for being used as bioindicators makes them integral to assessing mangrove health. Yet, their diversity and distribution patterns are not well understood. The aim of this study …

Macroinvertebrate Community And Temperature Changes In A Michigan Stream, Nyika Campbell

Independent Study Project (ISP) Collection

As the climate warms, global biodiversity is plummeting and extinction rates are rising (Jenkins, 2003). Freshwater ecosystems specifically are experiencing huge losses in diversity (Jenkins, 2003; Ormerod et al., 2010; Collier et al., 2016). Conservation of invertebrates is particularly urgent because they are foundational to the trophic systems in streams and lakes, comprising 95% of all species on earth (Titley et al., 2017), and over 60% of biodiversity in freshwater ecosystems (Collier et al., 2016). Through analysis of both my own field measurements and historical data I seek to gain a preliminary understanding of temperature and macroinvertebrate community changes that …

Parásitos Gastrointestinales De Tierra Del Fuego, Argentina, Sarah Martínez

Independent Study Project (ISP) Collection

Este trabajo intentó encontrar helmintos comunes, especialmente compartidos, en cuatro carnívoros patagónicos del sur con distribuciones geográficas superpuestas y dos carnívoros patagónicos del norte, y esbozar el análisis fecal y los métodos de identificación morfológica para futuros investigadores. El estudiante encontró solo un parásito helminto compartido entre cualquier otra especie y la nutria nativa del sur del río, a pesar de una superposición en el hábitat con el visón americano invasivo, pero esto puede deberse a un bajo recuento de estudios regionales previos de helmintos en cualquiera de las especies. Se encontraron múltiples parásitos en las cuatro especies hospederas de …

Cambios En El Cronograma De Temporadas Reproductivas De Pingüinos De Magallanes (Spheniscus Magellanicus): Relaciones Entre Eventos Fenológicos Y Variables Climáticas, Grace Neumiller

Independent Study Project (ISP) Collection

Estudios a largo plazo han demostrado que hay fluctuaciones a lo largo de la fenología de la vida silvestre a causa de los cambios climáticos (Black, 2016). Los pingüinos de Magallanes (Spheniscus magellanicus) exhiben cambios en el cronograma de los eventos fenológicos de sus temporadas reproductivas a causa de variables oceanográficas y climáticas de su ambiente. A partir de fotos de cámaras trampas, este trabajo describe y analice algunos eventos en la fenología de los pingüinos de Magallanes en Isla Martillo, Canal Beagle, Tierra del Fuego, Argentina: el inicio de la temporada reproductiva, el periodo de muda de pichones, y …

The Influence Of Location, Positioning, And Seasonality On Feeding Behavior Of The Sydney Oyster (Saccostrea Glomerata) In New South Wales, Australia, Nathaniel Hess

Independent Study Project (ISP) Collection

Oysters are hard shelled bivalves that aggregate over time to create structures in estuarine systems, known as oyster reefs. These reefs are important for the promotion of estuarine biodiversity by supporting many species of fish, invertebrates, and plants. They also act as a key contributor to water health by using active suspension feeding mechanisms and selective feeding to remove nutrients and water borne pollutants from estuarine systems. They have been touted as possible bio-remediators. They also effect rates of sedimentation in estuaries.

Oyster reefs have historically been threatened by anthropogenic influences such as overharvesting, destructive fishing practices, water pollution, CO2 …

A Preliminary Study For The Future Translocation Of A Saimiri Sciureus Troop From Sumak Allpa To Yasuní National Park: Assessing The Habitat Use, Population, And Behavior Of A Common Squirrel Monkey Troop In Indillama, Kenia French

Independent Study Project (ISP) Collection

Sumak Allpa, an organization specializing in primate rehabilitation, plans to introduce a Common Squirrel Monkey Troop ( Saimiri sciureus ), the Yasuní Troop, to the Indillama region of Yasuní National Park. This study analyzes the habitat use, population, and behavior of the Saimiri sciureus troop, referred to as the Indillama Troop, already existing in the region, from April 14th to May 4th, 2019. Focal and scan observation techniques were used to observe the troop’s behavior, and EasyTrails on an iPhone 7 was used to record GPS data. EasyTrails was also used to map the trail system in the Indillama region. …

Mammals Of The Northern Andes: An Analysis Of Camera Trap Data And Observation In Angochagua, Ecuador, Risa Berman

Independent Study Project (ISP) Collection

The high Andes of Ecuador are home to a diverse community of mammals, many of which are threatened. These include the Andean bear, Andean fox, puma, and mountain tapir. This study used camera traps, direct observation, and indirect observation over three weeks to monitor the wildlife in the mountains of the Parroquia of Angochagua. The five species observed during this period were Andean bear, Andean fox, dog, mountain paca, and mouse. While this short study did not completely survey the mammalian community of the area, it confirmed the presence, diet, sociality, temporal habits, elevational range, and habitat preferences of the …